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https://www.readbyqxmd.com/read/29240250/microrna-139-5p-inhibits-epithelial-mesenchymal-transition-and-fibrosis-in-post-menopausal-women-with-interstitial-cystitis-by-targeting-lpar4-via-the-pi3k-akt-signaling-pathway
#1
Chen Jiang, Zhen Tong, Wei-Lin Fang, Qi-Bo Fu, Yin-Jun Gu, Ting-Ting Lv, Dong-Ming Liu, Wei Xue, Jian-Wei Lv
The study explores whether miR-139-5p targeting LPAR4 affects epithelial-mesenchymal transition (EMT) and fibrosis in post-menopausal women with interstitial cystitis (IC) via the PI3K/Akt signaling pathway. Bladder tissues of IC and normal bladder tissues were collected. The pathology of bladder tissues was observed by HE, Masson and Picrosirius red staining. LPAR4 positive expression rate were determined by IHC. ELISA was performed to detect the levels of IL-6, IL-8, IL-10 and TNF-α. Rat IC models were randomized into seven different groups...
December 14, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29232016/characterization-of-h3-3-and-hira-expression-and-function-in-bovine-early-embryos
#2
Kun Zhang, Han Wang, Sandeep K Rajput, Joseph K Folger, George W Smith
Histone variant H3.3 is encoded by two distinct genes, H3F3A and H3F3B, that are closely associated with actively transcribed genes. H3.3 replacement is continuous and essential for maintaining correct chromatin structure during mouse oogenesis. Upon fertilization, H3.3 is incorporated to parental chromatin, and is required for blastocyst formation in mice. The H3.3 exchange process is facilitated by the chaperone HIRA, particularly during zygote development. We previously demonstrated that H3.3 is required for bovine early embryonic development; here, we explored the mechanisms of its functional requirement...
December 12, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29228554/systemic-analysis-of-gene-expression-profiles-in-porcine-granulosa-cells-during-aging
#3
Li Hui, Guo Shuangshuang, Yu Jianning, Shi Zhendan
Current studies have revealed that aging is a negative factor that suppresses granulosa cell functions and causes low fertility in women. However, the difference in gene expression between normal and aging granulosa cells remains undefined. Therefore, the aim of this study was to investigate the gene expression profiles of granulosa cells during aging. Granulosa cells from young healthy porcine ovaries were aged in vitro by prolonging the culture time (for 48h). First, the extracellular ultrastructure was observed by scanning electron microscopy followed by RNA-seq and KEGG pathway analysis...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29223655/in%C3%A2-vitro-cultured-bovine-endometrial-cells-recognize-embryonic-sex
#4
E Gómez, M J Sánchez-Calabuig, D Martin, S Carrocera, A Murillo, E Correia-Alvarez, P Herrero, N Canela, A Gutiérrez-Adán, S Ulbrich, M Muñoz
Endometrial cell co-culture (ECC) with single embryo may reflect endometrium responses in vivo. Bovine Day-6 in vitro-produced morulae were cultured until Day-8 in modified synthetic oviductal fluid (mSOF), or on the epithelial side of ECC. Expression of epithelial- and stromal-cell transcripts was analyzed by RT-PCR in ECC with one male (ME) or female embryo (FE). Concentrations of ARTEMIN (ARTN) and total protein were determined in epithelial cell-conditioned medium. ECCs yielded embryos with more cells in the inner cell mass than embryos cultured in mSOF...
December 5, 2017: Theriogenology
https://www.readbyqxmd.com/read/29219182/the-emerging-role-of-hippo-signaling-pathway-in-regulating-osteoclast-formation
#5
REVIEW
Wanlei Yang, Weiqi Han, An Qin, Ziyi Wang, Jiake Xu, Yu Qian
A delicate balance between osteoblastic bone formation and osteoclastic bone resorption is crucial for bone homeostasis. This process is regulated by the Hippo signaling pathway including key regulatory molecules RASSF2, NF2, MST1/2, SAV1, LATS1/2, MOB1, YAP and TAZ. It is well established that the Hippo signaling pathway plays an important part in regulating osteoblast differentiation, but its role in osteoclast formation and activation remains poorly understood. In this review, we discuss the emerging role of Hippo-signaling pathway in osteoclast formation and bone homeostasis...
December 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29215705/activation-of-fibroblasts-by-nicotine-promotes-the-epithelial-mesenchymal-transition-and-motility-of-breast-cancer-cells
#6
Pin-Cyuan Chen, Wen-Ying Lee, Hsiang-Hsi Ling, Chia-Hsiung Cheng, Ku-Chung Chen, Cheng-Wei Lin
The tumor microenvironment plays an important role in tumor initiation and progression. It is well documented that nicotine participates in cigarette smoking-related malignancies. Previous studies focused on the effects of nicotine on tumor cells; however, the role of the microenvironment in nicotine-mediated tumorigenesis is poorly understood. Herein, we investigated the effect and molecular mechanism of nicotine on fibroblasts and its contribution to breast cancer. We found that nicotine induced the epithelial-mesenchymal transition (EMT) of breast cancer cells and promoted activation of fibroblasts...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29214184/protective-effects-of-moringa-oleifera-on-hbv-genotypes-c-and-h-transiently-transfected-huh7-cells
#7
Sina Feustel, Fabiola Ayón-Pérez, Ana Sandoval-Rodriguez, Roberto Rodríguez-Echevarría, Homero Contreras-Salinas, Juan Armendáriz-Borunda, L V Sánchez-Orozco
Chronic hepatitis B infection treatment implicates a long-lasting treatment. M. oleifera extracts contain compounds with antiviral, antioxidant, and antifibrotic properties. In this study, the effect of M. oleifera was evaluated in Huh7 cells expressing either HBV genotypes C or H for the antiviral, antifibrotic, anti-inflammatory, and antioxidative responses. Huh7 cells were treated with an aqueous extract of M. oleifera (leaves) at doses of 0, 30, 45, or 60 μg/mL. The replicative virus and TGF-β1, CTGF, CAT, IFN-β1, and pgRNA expressions were measured by real time...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/29207016/microrna-21-promotes-the-progression-of-peritoneal-fibrosis-through-the-activation-of-the-tgf-%C3%AE-smad-signaling-pathway-an-in-vitro-and-in-vivo-study
#8
Ya-Li Ma, Fang Chen, Su-Xia Yang, Bao-Ping Chen, Jun Shi
The present study aimed to explore the roles of microRNA-21 (miR‑21) and the transforming growth factor-β (TGF-β)/Smad signaling pathway in the development of peritoneal fibrosis (PF). First, dialysis effluents from 30 patients with PF were collected, and after the establishment of a mouse model of PF, hematoxylin and eosin (H&E) and Masson's staining were used to observe peritoneal tissues, inflammatory cells and blood vessels. High glucose was used to stimulate human peritoneal mesothelial cell lines and these stimulated cells were then transfected with miR‑21 inhibitor...
November 17, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29198711/effects-of-connective-tissue-growth-factor-ctgf-ccn2-on-condylar-chondrocyte-proliferation-migration-maturation-differentiation-and-signalling-pathway
#9
Xin Xing, Zhi Li, Zili Yu, Gu Cheng, Dianqi Li, Zubing Li
CCN2, also known as connective tissue growth factor (CTGF), is a 38 kDa cysteine-rich extracellular matrix protein that regulates a sequence of cellular functions and participates in multiple complex biological processes, such as chondrogenesis and osteogenesis. In the present study, we provided the first evidence describing the physiological role of CCN2 in condylar chondrocyte proliferation, migration, maturation and differentiation. CCN2 was widely expressed throughout the whole layers of condylar cartilage and predominantly distributed in the proliferative zone...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29190236/apoptosis-and-angiofibrosis-in-diabetic-tractional-membranes-after-vascular-endothelial-growth-factor-inhibition-results-of-a-prospective-trial-report-no-2
#10
Chunhua Jiao, Dean Eliott, Christine Spee, Shikun He, Kai Wang, Robert F Mullins, David R Hinton, Elliott H Sohn
PURPOSE: We sought to characterize the angiofibrotic and apoptotic effects of vascular endothelial growth factor (VEGF)-inhibition on fibrovascular epiretinal membranes in eyes with traction retinal detachment because of proliferative diabetic retinopathy. METHODS: Membranes were excised from 20 eyes of 19 patients (10 randomized to intravitreal bevacizumab, 10 controls) at vitrectomy. Membranes were stained with antibodies targeting connective tissue growth factor (CTGF) or VEGF and colabeled with antibodies directed against endothelial cells (CD31), myofibroblasts, or retinal pigment epithelium markers...
November 22, 2017: Retina
https://www.readbyqxmd.com/read/29187583/mir-375-attenuates-injury-of-cerebral-ischemia-reperfusion-via-targeting-ctgf
#11
Jianying Ou, Li Kou, Lingyan Liang, Chaogang Tang
Ischemic stroke is the leading cause of disability and death worldwide. MicroRNAs have been shown to play an important role in development and pathogenesis of the nervous system. However, the precise function and mechanism of miRNAs are not fully understood in the brain injury induced by ischemia/reperfusion (I/R). Herein, our study showed that miR-375 expression was significantly down-regulated in the rat I/R brain. With the in vivo and in vitro I/R stroke models we found that miR-375 mimic provides significant protection from injury of cerebral I/R, which is reflected by reduced infarct volumes and cell apoptosis, increased proliferation and migration of PC12 cells...
November 29, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29184496/andrographolide-protects-against-aortic-banding-induced-experimental-cardiac-hypertrophy-by-inhibiting-mapks-signaling
#12
Qing Q Wu, Jian Ni, Ning Zhang, Hai H Liao, Qi Z Tang, Wei Deng
Despite therapeutic advances, heart failure-related mortality rates remain high. Therefore, understanding the pathophysiological mechanisms involved in the remodeling process is crucial for the development of new therapeutic strategies. Andrographolide (Andr), a botanical compound, has potent cardio-protective effects due to its ability to inhibit mitogen-activated protein kinases (MAPKs). Andr has also been shown to inhibit inflammation and apoptosis, which are factors related to cardiac hypertrophy. Our aim was to evaluate the effects of Andr on cardiac hypertrophy and MAPKs activation...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29179208/klf-15-works-as-an-early-anti-fibrotic-transcriptional-regulator-in-ang-ii-induced-renal-fibrosis-via-down-regulation-of-ctgf-expression
#13
Xiangchen Gu, Dechao Xu, Lili Fu, Yi Wang, Changlin Mei, Xiang Gao
BACKGROUND/AIMS: Angiotensin II (Ang II) has been regarded as an important profibrogenic cytokine in renal fibrosis. Krüppel-like factor 15 (KLF15) has been identified as an important negative transcription factor in renal fibrosis. However, little is known about the role of KLF15 in Ang II-induced renal fibrosis. METHODS: In this study, we randomized mice into a control group, Ang II group or Ang II plus losartan group. KLF15 expression was examined with real-time PCR and immunofluorescence in these groups...
November 27, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29179163/-the-role-of-osteocytes-in-bone-remodeling
#14
Takashi Nishida, Satoshi Kubota, Masaharu Takigawa
Bone remodeling has important roles in the functions of bone tissues, such as supporting the body and mineral storage. Osteocytes, which are the most abundant cells in bone tissues, detect the mechanical loading and regulate both bone formation by osteoblasts and bone resorption by osteoclasts. However, its mechanism is still unknown. In this review, we summarize how osteocytes detect mechanical stress and discuss the potential role of CCN2/CTGF as a novel bone remodeling factor produced by osteocytes.
2017: Clinical Calcium
https://www.readbyqxmd.com/read/29177025/acute-exposure-to-cigarette-smoking-followed-by-myocardial-infarction-aggravates-renal-damage-in-an-in-vivo-mouse-model
#15
Firas Kobeissy, Abdullah Shaito, Abdullah Kaplan, Lama Baki, Hassan Hayek, Carole Dagher-Hamalian, Ali Nehme, Rana Ghali, Emna Abidi, Ahmad Husari, Asad Zeidan, Fouad A Zouein, Kazem Zibara
Cigarette smoking (S) is a risk factor for progressive chronic kidney disease, renal dysfunction, and renal failure. In this study, the effect of smoking on kidney function was investigated in a mouse model of myocardial infarction (MI) using 4 groups: control (C), smoking (S), MI, and S+MI. Histological analysis of S+MI group showed alterations in kidney structure including swelling of the proximal convoluted tubules (PCTs), thinning of the epithelial lining, focal loss of the brush border of PCTs, and patchy glomerular retraction...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29175126/immunolocalization-of-connective-tissue-growth-factor-transforming-growth-factor-beta1-and-phosphorylated-smad2-3-during-the-postnatal-tooth-development-and-formation-of-junctional-epithelium
#16
Shubo Li, Yihuai Pan
Connective tissue growth factor (CTGF) is a downstream mediator of transforming growth factor-beta 1 (TGF-β1) and TGF-β1-induced CTGF expression is regulated through SMAD pathway. However, there is no literature showing the expression of TGF-β1-SMAD2/3-CTGF signaling pathway during postnatal tooth development and the formation of junctional epithelium (JE). Hence, we aimed to analyze the localization of TGF-β1, CTGF and phosphorylated SMAD2/3 (p-SMAD2/3) in the developing postnatal rat molars. Wistar rats were killed at postnatal (PN) 0...
November 21, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29174694/synergistic-promoting-effects-of-bone-morphogenetic-protein-12-connective-tissue-growth-factor-on-functional-differentiation-of-tendon-derived-stem-cells-and-patellar-tendon-window-defect-regeneration
#17
Kang Xu, Yanjun Sun, Mohanad Kh Al-Ani, Chunli Wang, Yongqiang Sha, Kl Paul Sung, Nianguo Dong, Xuefeng Qiu, Li Yang
Current study investigated bone morphogenetic protein 12 (BMP12) and connective tissue growth factor (CTGF) activate tendon derived stem cells (TDSCs) tenogenic differentiation, and promotion of injured tendon regeneration. TDSCs were transfected with BMP12 and CTGF via recombinant adenovirus (Ad) infection. Gene transfection efficiency, cell viability and cytotoxicity, tenogenic gene expression, collagen I/III synthesis were evaluated in vitro. For the in vivo study, the transfected cells were transplanted into the rat patellar tendon window defect...
November 7, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29166915/serum-connective-tissue-growth-factor-is-a-highly-discriminatory-biomarker-for-the-diagnosis-of-rheumatoid-arthritis
#18
Xinyu Yang, Ke Lin, Shanmin Ni, Jianmin Wang, Qingqing Tian, Huaijun Chen, Matthew A Brown, Kaidi Zheng, Weitao Zhai, Li Sun, Shengwei Jin, Jianguang Wang
BACKGROUND: Our previous proteomic study indicated that connective tissue growth factor (CTGF) may be a potential biomarker for rheumatoid arthritis (RA) diagnosis. The aim was to assess the performance of CTGF as a biomarker of RA. METHOD: Serum and synovial fluid CTGF was detected using a direct high sensitivity sandwich ELISA kit. Serum CTGF levels were tested for discriminatory capacity and optimal assay cutoffs determined in a training cohort of 98 cases of RA with 103 healthy controls...
November 22, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/29165602/deletion-of-connective-tissue-growth-factor-ameliorates-peritoneal-fibrosis-by-inhibiting-angiogenesis-and-inflammation
#19
Naohiro Toda, Kiyoshi Mori, Masato Kasahara, Kenichi Koga, Akira Ishii, Keita P Mori, Keisuke Osaki, Masashi Mukoyama, Motoko Yanagita, Hideki Yokoi
Background: Connective tissue growth factor (CTGF/CCN2) regulates the signalling of other growth factors and promotes fibrosis. CTGF is increased in mice and humans with peritoneal fibrosis. Inhibition of CTGF has not been examined as a potential therapeutic target for peritoneal fibrosis because systemic CTGF knockout mice die at the perinatal stage. Methods: To study the role of CTGF in peritoneal fibrosis of adult mice, we generated CTGF conditional knockout (cKO) mice by crossing CTGF floxed mice with RosaCreERT2 mice...
November 17, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29160908/connective-tissue-growth-factor-induces-renal-fibrosis-via-epidermal-growth-factor-receptor-activation
#20
Sandra Rayego-Mateos, José Luis Morgado-Pascual, Raúl R Rodrigues-Diez, Raquel Rodrigues-Diez, Lucas Falke, Sergio Mezzano, Alberto Ortiz, Jesús Egido, Roel Goldschmeding, Marta Ruiz-Ortega
Connective tissue growth factor (CCN2/CTGF) is a matricellular protein overexpressed in progressive human renal diseases, mainly in fibrotic areas. In vitro studies have demonstrated that CCN2 regulates production of extracellular matrix (ECM) proteins and epithelial mesenchymal transition (EMT), and therefore could contribute to renal fibrosis. CCN2 blockade ameliorates experimental renal damage, including diminution of ECM accumulation. We have described that CCN2 and its C-terminal degradation product CCN2(IV), bind to epidermal growth factor receptor (EGFR) to modulate renal inflammation...
November 21, 2017: Journal of Pathology
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