keyword
MENU ▼
Read by QxMD icon Read
search

CTGF

keyword
https://www.readbyqxmd.com/read/28092445/mir-29b-promotes-skin-wound-healing-and-reduces-excessive-scar-formation-by-inhibition-of-the-tgf-%C3%AE-1-smad-ctgf-signaling-pathway
#1
Jingdong Guo, Quan Lin, Ying Shao, Li Rong, Duo Zhang
The hypertrophic scar is a medical difficulty of humans, which has caused great pain to patients. Here, we investigated the inhibitory effect of miR-29b on scar formation. The scalded model was established in mice and miR-29b mimics or a negative control was subcutaneously injected into the injury skin. Then various molecular biological experiments were performed to assess the effect of miR-29b on scar formation. According to our present study, first, the results demonstrated that miR-29b was down-regulated in thermal injury tissue and miR-29b treatment could promote wound healing, inhibit scar formation, and alleviate histopathological morphologic alteration in scald tissues...
November 24, 2016: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28089819/lentivirus-mediated-silencing-of-the-ctgf-gene-suppresses-the-formation-of-glial-scar-tissue-in-a-rat-model-of-spinal-cord-injury
#2
Yuan Wang, Qin-Jie Kong, Jing-Chuan Sun, Yong Yang, Hai-Bo Wang, Qiang Zhang, Jian-Gang Shi
BACKGROUND CONTEXT: - One of the many reactive changes following an Spinal cord injury (SCI) is the formation of a glial scar, a reactive cellular process whereby glial cells accumulate and surround central nervous system (CNS) injury sites to seal in the wound. Thus the inhibition of glial scar is of great importance for SCI recovery. PURPOSE: - This study aimed to explore the effect of lentivirus-mediated silencing of the CTGF gene on the formation of glial scar tissue in a rat model of spinal cord injury (SCI)...
January 9, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28079129/circrna_000203-enhances-the-expression-of-fibrosis-associated-genes-by-derepressing-targets-of-mir-26b-5p-col1a2-and-ctgf-in-cardiac-fibroblasts
#3
Chun-Mei Tang, Ming Zhang, Lei Huang, Zhi-Qin Hu, Jie-Ning Zhu, Zhen Xiao, Zhuo Zhang, Qiu-Xiong Lin, Xi-Long Zheng, Min -Yang, Shu-Lin Wu, Jian-Ding Cheng, Zhi-Xin Shan
Circular RNAs (circRNAs) participate in regulating gene expression in diverse biological and pathological processes. The present study aimed to investigate the mechanism underlying the modulation of circRNA_000203 on expressions of fibrosis-associated genes in cardiac fibroblasts. CircRNA_000203 was shown upregulated in the diabetic mouse myocardium and in Ang-II-induced mouse cardiac fibroblasts. Enforced-expression of circRNA_000203 could increase expressions of Col1a2, Col3a1 and α-SMA in mouse cardiac fibroblasts...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28076452/angiotensin-1-7-relieved-renal-injury-induced-by-chronic-intermittent-hypoxia-in-rats-by-reducing-inflammation-oxidative-stress-and-fibrosis
#4
W Lu, J Kang, K Hu, S Tang, X Zhou, S Yu, L Xu
We aimed to study the renal injury and hypertension induced by chronic intermittent hypoxia (CIH) and the protective effects mediated by angiotensin 1-7 [Ang(1-7)]. We randomly assigned 32 male Sprague-Dawley rats (body weight 180-200 g) to normoxia control, CIH, Ang(1-7)-treated normoxia, and Ang(1-7)-treated CIH groups. Systolic blood pressure (SBP) was monitored at the start and end of each week. Renal sympathetic nerve activity (RSNA) was recorded. CTGF and TGF-β were detected by immunohistochemistry and western blotting...
January 9, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28067893/vemurafenib-and-trametinib-reduce-expression-of-ctgf-and-il-8-in-v600e-braf-melanoma-cells
#5
Mariusz L Hartman, Michal Rozanski, Marta Osrodek, Izabela Zalesna, Malgorzata Czyz
Clinical evidence has revealed that while RAS/RAF/MEK/ERK pathway is a crucial component of melanomagenesis, other signaling pathways can also contribute to the malignant growth and development of resistance to targeted therapies. We explored the response of (V600E)BRAF melanoma cells derived from surgical specimens and grown in stem cell medium to vemurafenib and trametinib, drugs targeting the activity of (V600E)BRAF and MEK1/2, respectively. Cell growth and apoptosis were monitored by real-time imaging system, immunophenotype and cell cycle by flow cytometry, gene expression by quantitative real-time PCR, immunoblotting and enzyme-linked immunosorbent assay...
January 9, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28039692/biological-characteristics-of-ccn-proteins-in-tumor-development
#6
Stephany C Barreto, Amitabha Ray, Elena G Geromichalou, Page Ag Edgar
The extracellular membrane (ECM) is no longer regarded as inert, rather it has multiple versatile physiologic functions. Its diverse composition is implicated in each step of cancer progression including inflammation, angiogenesis, tumor invasion and metastasis. In addition to structural proteins, the ECM also contains a family of non-structural proteins called matricellular proteins. The six secreted CCN proteins, which belong to the matricellular protein family, include the following: Cyr61 (CCN1), CTGF (CCN2), Nov (CCN3), WISP- 1 (CCN4), WISP-2 (CCN5) and WISP-3(CCN6)...
November 2016: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28026993/connective-tissue-growth-factor-promotes-pulmonary-epithelial-cell-senescence-and-is-associated-with-copd-severity
#7
Jun-Ho Jang, Hitendra S Chand, Shannon Bruse, Melanie Doyle-Eisele, Christopher Royer, Jacob McDonald, Clifford Qualls, Aloysius J Klingelhutz, Yong Lin, Rama Mallampalli, Yohannes Tesfaigzi, Toru Nyunoya
The purpose of this study was to determine whether expression of connective tissue growth factor (CTGF) protein in chronic obstructive pulmonary disease (COPD) is consistent in humans and animal models of COPD and to investigate the role of this protein in lung epithelial cells. CTGF in lung epithelial cells of ex-smokers with COPD was compared with ex-smokers without COPD by immunofluorescence. A total of twenty C57Bl/6 mice and sixteen non-human primates (NHPs) were exposed to cigarette smoke (CS) for 4 weeks...
December 27, 2016: COPD
https://www.readbyqxmd.com/read/28011198/cell-autonomous-antxr1-mediated-regulation-of-extracellular-matrix-components-in-primary-fibroblasts
#8
Kai Hu, Bjorn R Olsen, Tatiana Y Besschetnova
Our previous studies of Antxr1 knockout mice suggested that fibrotic skin abnormalities in these mice are associated with increased VEGF signaling. Here, based on studies of primary fibroblasts isolated from skin of Antx1+/+ and Antxr1-/- mice at embryonic stage E17.5 and postnatal day P49, we conclude that increased Col1a1 and Fn1 expression in Antxr1-deficient fibroblasts is partly mediated by a cell-autonomous ANTXR1-dependent mechanism. In turn, this may act in parallel with VEGF-dependent regulation of collagen type I and fibronectin production...
December 20, 2016: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28005267/human-gingiva-transcriptome-during-wound-healing
#9
Yun Wang, Dimitris N Tatakis
OBJECTIVES: To investigate the gene expression profile of human gingiva following surgical wounding. METHODS: Ten volunteers had one side of the palate wounded. Five days later, biopsies were harvested from both wounded (healing gingiva) and contralateral site (normal gingiva). Tissue samples were processed for gene expression (RNA-Seq, real-time PCR) and immuno-histochemistry. Gene-set enrichment/pathway analysis was also performed. RESULTS: Seven hundred genes were significantly differentially expressed in healing gingiva...
December 22, 2016: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/27999189/mesenchymal-marker-expression-is-elevated-in-m%C3%A3-ller-cells-exposed-to-high-glucose-and-in-animal-models-of-diabetic-retinopathy
#10
Ti Zhou, Di Che, Yuqing Lan, Zhenzhen Fang, Jinye Xie, HaiJun Gong, ChaoYang Li, Juan Feng, Honghai Hong, Weiwei Qi, Caiqi Ma, Zhonghan Yang, WeiBin Cai, Jun Zhong, Jianxing Ma, Xia Yang, Guoquan Gao
Müller cells are retinal glial cells and exhibit a fibroblast-like phenotype and ability to migrate in diabetic retinopathy (DR). However, expression of mesenchymal markers, which promote fibrosis in various organs, has not been characterized in the diabetic retina. We examined changes in the expression of these markers in Müller cells exposed to high glucose and in animal models of diabetic retinopathy. High glucose conditions increased mesenchymal maker expression and migration in Müller cells. Snail, N-cadherin, Vimentin, β-catenin, and α-smooth muscle actin (α-SMA) levels were all dramatically increased in retinas from humans with diabetic retinopathy (DR) and from DR mouse models...
December 15, 2016: Oncotarget
https://www.readbyqxmd.com/read/27992438/simultaneous-administration-of-adscs-based-therapy-and-gene-therapy-using-ad-hupa-reduces-experimental-liver-fibrosis
#11
Alejandra Meza-Ríos, Leonel García-Benavides, Jesus García-Bañuelos, Adriana Salazar-Montes, Juan Armendáriz-Borunda, Ana Sandoval-Rodríguez
BACKGROUND AND AIMS: hADSCs transplantation in cirrhosis models improves liver function and reduces fibrosis. In addition, Ad-huPA gene therapy diminished fibrosis and increased hepatocyte regeneration. In this study, we evaluate the combination of these therapies in an advanced liver fibrosis experimental model. METHODS: hADSCs were expanded and characterized before transplantation. Ad-huPA was simultaneously administrated via the ileac vein. Animals were immunosuppressed by CsA 24 h before treatment and until sacrifice at 10 days post-treatment...
2016: PloS One
https://www.readbyqxmd.com/read/27987320/association-between-axl-hippo-transducers-and-survival-outcomes-in-male-breast-cancer
#12
Anna Di Benedetto, Marcella Mottolese, Francesca Sperati, Cristiana Ercolani, Luigi Di Lauro, Laura Pizzuti, Patrizia Vici, Irene Terrenato, Abeer M Shaaban, Matthew P Humphries, Sreekumar Sundara-Rajan, Maddalena Barba, Valerie Speirs, Ruggero De Maria, Marcello Maugeri-Saccà
Male breast cancer (MBC) is an uncommon malignancy. We have previously reported that the expression of the Hippo transducers TAZ/YAP and their target CTGF was associated with inferior survival in MBC patients. Preclinical evidence demonstrated that Axl is a transcriptional target of TAZ/YAP. Thus, we herein assessed AXL expression to further investigate the significance of active TAZ/YAP-driven transcription in MBC. For this study, 255 MBC samples represented in tissue microarrays were screened for AXL expression, and 116 patients were included...
December 17, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27983601/transcriptome-of-cultured-lung-fibroblasts-in-idiopathic-pulmonary-fibrosis-meta-analysis-of-publically-available-microarray-datasets-reveals-repression-of-inflammation-and-immunity-pathways
#13
Laurent Plantier, Hélène Renaud, Renaud Respaud, Sylvain Marchand-Adam, Bruno Crestani
Heritable profibrotic differentiation of lung fibroblasts is a key mechanism of idiopathic pulmonary fibrosis (IPF). Its mechanisms are yet to be fully understood. In this study, individual data from four independent microarray studies comparing the transcriptome of fibroblasts cultured in vitro from normal (total n = 20) and IPF (total n = 20) human lung were compiled for meta-analysis following normalization to z-scores. One hundred and thirteen transcripts were upregulated and 115 were downregulated in IPF fibroblasts using the Significance Analysis of Microrrays algorithm with a false discovery rate of 5%...
December 13, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27965087/ship-a-novel-factor-to-ameliorate-extracellular-matrix-accumulation-via-suppressing-pi3k-akt-ctgf-signaling-in-diabetic-kidney-disease
#14
Fan Li, Lisha Li, Meijuan Cheng, Xiumin Wang, Jun Hao, Shuxia Liu, Huijun Duan
Tubular interstitial extracellular matrix accumulation, which plays a key role in the pathogenesis and progression of diabetic kidney disease (DKD), is believed to be mediated by activation of PI3K/Akt signal pathway. However, it is still not clear whether SH2 domain-containing inositol 5'-phosphatase (SHIP), known as a negative regulator of PI3K/Akt pathway is also involved in extracellular matrix metabolism of diabetic kidney. In the present study, decreased SHIP and increased phospho-Akt (Ser 473, Thr 308) were found in renal tubular cells of diabetic mice accompanied by overexpression of connective tissue growth factor (CTGF) and extracellular matrix deposition versus normal mice...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27942222/ctgf-enhances-resistance-to-5-fu-mediating-cell-apoptosis-through-fak-mek-erk-signal-pathway-in-colorectal-cancer
#15
Kai Yang, Kai Gao, Gui Hu, Yanguang Wen, Changwei Lin, Xiaorong Li
Colorectal cancer (CRC) is one of the most commonly diagnosed cancers among both males and females; the chemotherapy drug 5-fluorouracil (5-FU) is one of a doctors' first lines of defense against CRC. However, therapeutic failures are common because of the emergence of drug resistance. Connective tissue growth factor (CTGF) is a secreted protein that binds to integrins, and regulates the invasiveness and metastasis of certain carcinoma cells. Here, we found that CTGF was upregulated in drug-resistant phenotype of human CRC cells...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27941938/effects-of-exendin-4-on-human-adipose-tissue-inflammation-and-ecm-remodelling
#16
E Pastel, S Joshi, B Knight, N Liversedge, R Ward, K Kos
BACKGROUND/OBJECTIVES: Subjects with type-2 diabetes are typically obese with dysfunctional adipose tissue (AT). Glucagon-like peptide-1 (GLP-1) analogues are routinely used to improve glycaemia. Although, they also aid weight loss that improves AT function, their direct effect on AT function is unclear. To explore GLP-1 analogues' influence on human AT's cytokine and extracellular matrix (ECM) regulation, we therefore obtained and treated omental (OMAT) and subcutaneous (SCAT) AT samples with Exendin-4, an agonist of the GLP-1 receptor (GLP-1R)...
December 12, 2016: Nutrition & Diabetes
https://www.readbyqxmd.com/read/27941433/kidney-fibrosis-origins-and-interventions
#17
Thomas Vanhove, Roel Goldschmeding, Dirk Kuypers
All causes of renal allograft injury, when severe and/or sustained, can result in chronic histological damage of which interstitial fibrosis and tubular atrophy (IF/TA) are dominant features. Unless a specific disease process can be identified, what drives IF/TA progression in individual patients is often unclear. In general, clinicopathological factors known to predict and drive allograft fibrosis include graft quality, inflammation (whether 'nonspecific' or related to a specific diagnosis), infections such as polyomavirus-associated nephropathy, calcineurin inhibitors and genetic factors...
December 8, 2016: Transplantation
https://www.readbyqxmd.com/read/27923814/mir-483-targeting-of-ctgf-suppresses-endothelial-to-mesenchymal-transition-therapeutic-implications-in-kawasaki-disease
#18
Ming He, Zhen Chen, Marcy Martin, Jin Zhang, Panjamaporn Sangwung, Brian Woo, Adriana Tremoulet, Chisato Shimizu, Mukesh K Jain, Jane C Burns, John Shyy
RATIONALE: Endothelial-to-mesenchymal transition (EndoMT) is implicated in myofibroblast-like cell-mediated damage to the coronary arterial wall in acute Kawasaki disease (KD) patients, as evidenced by positive staining for connective tissue growth factor (CTGF) and EndoMT markers in KD autopsy tissues. However, little is known about the molecular basis of EndoMT involved in KD. OBJECTIVE: We investigated the microRNA (miRNA) regulation of CTGF and the consequent EndoMT in KD pathogenesis...
December 6, 2016: Circulation Research
https://www.readbyqxmd.com/read/27902771/piceatannol-attenuates-renal-fibrosis-induced-by-unilateral-ureteral-obstruction-via-downregulation-of-histone-deacetylase-4-5-or-p38-mapk-signaling
#19
Sin Young Choi, Zhe Hao Piao, Li Jin, Jung Ha Kim, Gwi Ran Kim, Yuhee Ryu, Ming Quan Lin, Hyung-Seok Kim, Hae Jin Kee, Myung Ho Jeong
Piceatannol, a resveratrol metabolite, is a phenolic compound found in red wine and grapes. We investigated the effect of piceatannol on renal fibrosis and histone deacetylase (HDAC) expression in a mouse model of unilateral ureteral obstruction (UUO). Fibrosis was established by UUO and piceatannol was intraperitoneally injected for 2 weeks. Piceatannol suppressed extracellular matrix (ECM) protein deposition including collagen type I and fibronectin as well as connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in UUO kidneys...
2016: PloS One
https://www.readbyqxmd.com/read/27894619/expression-of-pro-fibrotic-and-anti-fibrotic-molecules-in-dimethylnitrosamine-induced-hepatic-fibrosis
#20
Roberta Sferra, Antonella Vetuschi, Simona Pompili, Eugenio Gaudio, Silvia Speca, Giovanni Latella
BACKGROUND: Hepatic fibrosis is characterized by a progressive accumulation of fibrillar extracellular matrix (ECM) proteins, produced by activated myofibroblasts which are modulated by both profibrotic and antifibrotic factors. OBJECTIVE: To evaluate in vivo the expression of pro-fibrotic molecules like avβ6 integrin, transforming growth factor-β (TGF-β), Smad3, connective tissue growth factor (CTGF) and mammalian target of Rapamycin (mTOR), as well as anti-fibrotic peroxisome proliferator-activated receptor-γ (PPARγ) in an experimental model of chronic hepatitis-associated fibrosis induced by intraperitoneal administration of dimethylnitrosamine (DMN) in mice...
January 2017: Pathology, Research and Practice
keyword
keyword
30925
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"