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plasmacytoid dendritic cells

Raphael Teipel, Uta Oelschlägel, Katrin Wetzko, Maria Schmiedgen, Michael Kramer, Elke Rücker-Braun, Kristina Hölig, Malte von Bonin, Katharina Heidrich, Anke Fuchs, Rainer Ordemann, Frank Kroschinsky, Martin Bornhäuser, Gero Hütter, Helmuth Schmidt, Gerhard Ehninger, Johannes Schetelig, Falk Heidenreich
This study was conducted to characterize and compare peripheral blood stem cell grafts from healthy donors, who underwent G-CSF-mobilization and subsequently received one dose of plerixafor, after insufficient stem cell yields were achieved at the first apheresis. Aliquots from 35 donors were collected from the first apheresis after mobilization with G-CSF alone, and from the second apheresis after additional plerixafor administration. Samples were freshly analyzed for cellular subsets by 8-color flow-cytometry...
June 20, 2018: Biology of Blood and Marrow Transplantation
Anna Hammer, Anne Waschbisch, Kristina Kuhbandner, Antonios Bayas, De-Hyung Lee, Alexander Duscha, Aiden Haghikia, Ralf Gold, Ralf A Linker
Objective: Immunological studies have demonstrated a plethora of beneficial effects of dimethyl fumarate (DMF) on various cell types. However, the cellular and molecular targets are incompletely understood and response markers are scarce. Here, we focus on the relation between nuclear factor (erythroid-derived 2)-like 2 (NRF2) pathway induction under DMF therapy and the composition of the blood immune cell compartment and clinical efficacy in relapsing-remitting multiple sclerosis (MS) patients...
June 2018: Annals of Clinical and Translational Neurology
Keiko Aota, Tomoko Yamanoi, Koichi Kani, Koh-Ichi Nakashiro, Naozumi Ishimaru, Masayuki Azuma
BACKGROUND: Mechanisms underlying immune cells' recruitment and activation into the inflammatory lesions of lip salivary glands (LSGs) from primary Sjögren's syndrome (pSS) patients are incompletely understood. Chemokines play pivotal roles in these processes, so we investigated the clinical significance of chemokine receptor CXCR3 and its ligands in the autoimmune lesions of pSS. METHODS: We histologically determined the grade of LSG samples from 22 pSS patients and subjected the samples to immunofluorescence analysis to determine the expressions of CXCR3 and its ligands: CXCL9, CXCL10, and CXCL11...
June 21, 2018: Journal of Oral Pathology & Medicine
Patrick Fernandes Rodrigues, Llucia Alberti-Servera, Anna Eremin, Gary E Grajales-Reyes, Robert Ivanek, Roxane Tussiwand
Plasmacytoid dendritic cells (pDCs) are an immune subset devoted to the production of high amounts of type 1 interferons in response to viral infections. Whereas conventional dendritic cells (cDCs) originate mostly from a common dendritic cell progenitor (CDP), pDCs have been shown to develop from both CDPs and common lymphoid progenitors. Here, we found that pDCs developed predominantly from IL-7R+ lymphoid progenitor cells. Expression of SiglecH and Ly6D defined pDC lineage commitment along the lymphoid branch...
June 20, 2018: Nature Immunology
Markus G Manz
No abstract text is available yet for this article.
June 20, 2018: Nature Immunology
Sayaka Kimura, Tomoh Matsumiya, Yuko Shiba, Michi Nakanishi, Ryo Hayakari, Shogo Kawaguchi, Hidemi Yoshida, Tadaatsu Imaizumi
The recognition of nonself dsRNA by retinoic acid-inducible gene-I (RIG-I) leads to the engagement of RIG-I-like receptor signaling. In addition, nonself dsRNA triggers a robust latent RNase (RNase L) activation and leads to the degradation of ribosomal structures and cell death. In contrast, nonself ssRNA is known to be recognized by TLR 7/8 in immune cells such as plasmacytoid dendritic cells and B cells, but little is known regarding the involvement of nonself ssRNA in antiviral signaling in nonimmune cells, including epithelial cells...
June 20, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
S V Shirshev, E G Orlova, O A Loginova, I V Nekrasova, O L Gorbunova, I L Maslennikova
We studied the effect of hormones estriol, ghrelin, kisspeptin, and chorionic gonadotropin in concentrations corresponding to their content in the peripheral blood in each trimester of pregnancy on the expression of membrane molecules on myeloid and plasmacytoid dendritic cells of the thymus. It was found that thymic myeloid dendritic cells are sensitive to the action of estriol and kisspeptin. Estriol in a concentration of the first trimester of pregnancy reduces the number of myeloid dendritic cells expressing receptor for thymic stromal lymphopoietin (CD11c+TSLP-R+ ) and inhibitory molecule B7-H3 (CD11c+ CD276+ )...
June 19, 2018: Bulletin of Experimental Biology and Medicine
Brian Webster, Scott W Werneke, Biljana Zafirova, Sébastien This, Séverin Coléon, Elodie Décembre, Helena Paidassi, Isabelle Bouvier, Pierre-Emmanuel Joubert, Darragh Duffy, Thierry Walzer, Matthew L Albert, Marlène Dreux
Type I interferon (IFN-I) responses are critical for the control of RNA virus infections, however, many viruses, including Dengue (DENV) and Chikungunya (CHIKV) virus, do not directly activate plasmacytoid dendritic cells (pDCs), robust IFN-I producing cells. Herein, we demonstrated that DENV and CHIKV infected cells are sensed by pDCs, indirectly, resulting in selective IRF7 activation and IFN-I production, in the absence of other inflammatory cytokine responses. To elucidate pDC immunomodulatory functions, we developed a mouse model in which IRF7 signaling is restricted to pDC...
June 19, 2018: ELife
Nannan Han, Zun Zhang, Houyu Jv, Jingzhou Hu, Min Ruan, Chenping Zhang
OBJECTIVES: The aim of the present study was to investigate whether tumor-derived supernatants down-regulate the immune function of plasmacytoid dendritic cells (pDCs) in oral cancer and the potential molecular mechanisms of this effect. MATERIALS AND METHODS: Immunohistochemistry (IHC) and flow cytometry were used to detect tumor-infiltrating and peripheral blood pDCs. MTS and flow cytometry were employed to evaluate the immune response of CD4+ T cells. Real-time PCR and ELISA assays were used to identify TLR-7 and TLR-9 expression, IFN-α production and tumor-secreted soluble cytokines...
June 5, 2018: Archives of Oral Biology
Erica K Low, Egil Brudvik, Bradford Mitchell Kuhlman, Paul F Wilson, Graca M Almeida-Porada, Christopher D Porada
Astronauts on missions beyond low-Earth orbit are exposed to a hostile environment in which they are continually bombarded with unique high-energy species of radiation, while in conditions of microgravity (G), which can alter radiation response and immunity. In the present studies, we examined the impact exposing human hematopoietic stem/progenitor cells (HSC) to μG had upon their capacity to repair DNA damage and their ability to generate immune cells critical for mounting an effective anti-tumor response...
June 14, 2018: Stem Cells and Development
Eita Sasaki, Haruka Momose, Yuki Hiradate, Keiko Furuhata, Takuo Mizukami, Isao Hamaguchi
Safety evaluation of a human vaccine is critical for vaccine development and for preventing an unexpected adverse reaction in humans. Nonetheless, to date, very few systems have been described for preclinical studies of human adverse reactions in vivo . Previously, we have identified biomarker genes expressed in the lungs for evaluation of influenza vaccine safety, and their usefulness in rodent models and for adjuvant-containing vaccines has already been reported. Here, our purpose was to develop a novel humanized mouse model retaining human innate-immunity-related cells to assess the safety of influenza vaccines using the previously identified biomarker genes...
May 25, 2018: Oncotarget
Enping Hong, Justin Halman, Ankit Shah, Emil F Khisamutdinov, Marina A Dobrovolskaia, Kirill A Afonin
Nucleic acid nanoparticles (NANPs) have evolved as a new class of therapeutics with the potential to detect and treat diseases. Despite tremendous advancements in NANPs' development, their immunotoxicity, one of the major impediments in clinical translation of traditional therapeutic nucleic acids (TNAs), has never been fully characterized. Here, we describe the first systematically studied immunological recognition of 25 representative RNA and DNA NANPs selected to have different design principles and physicochemical properties...
June 12, 2018: Nano Letters
Ryutaro Iwabuchi, Shota Ikeno, Mie Kobayashi-Ishihara, Haruko Takeyama, Manabu Ato, Yasuko Tsunetsugu-Yokota, Kazutaka Terahara
Two cytokines, fms-related tyrosine kinase 3 ligand (Flt3-L) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are considered to be the essential regulators of dendritic cell (DC) development in vivo . However, the combined effect of Flt3-L and GM-CSF on human DCs has not been evaluated in vivo . In this study, we, therefore, aimed at evaluating this using a humanized mouse model. Humanized non-obese diabetic/SCID/Jak3null (hNOJ) mice were constructed by transplanting hematopoietic stem cells from human umbilical cord blood into newborn NOJ mice, and in vivo transfection (IVT) was performed by hydrodynamic injection-mediated gene delivery using plasmids encoding human Flt3-L and GM-CSF...
2018: Frontiers in Immunology
Renaud Felten, Elida Dervovic, François Chasset, Jacques-Eric Gottenberg, Jean Sibilia, Florence Scher, Laurent Arnaud
Currently, Systemic Lupus Erythematosus (SLE) therapies range from antimalarials to glucocorticoids, in addition to immunosupressive agents or biologics such as rituximab or belimumab, when needed. Several unmet needs remain in the treatment SLE and more targeted drugs with improved safety profiles are expected. Based on recent advances in the understanding of the complex pathogenesis of SLE, several targeted treatments are currently assessed in clinical trials. In this study, we performed a systematic review of all targeted therapies under clinical development in SLE in 17 online registries of clinical trials...
June 6, 2018: Autoimmunity Reviews
Lhara Sumarriva Lezama, Karen M Chisholm, Eugene Carneal, Alexandra Nagy, Michael J Cascio, Jie Yan, Chung-Che Chang, Athena Cherry, Tracy I George, Robert S Ohgami
AIM: Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is an aggressive neoplasm with leukemic features and frequent skin involvement. Translocations involving the MYC locus have been recently identified as a recurrent cytogenetic abnormality in this entity. Our aim is to assess the clinicopathologic, immunophenotypic, and genetic features in MYC rearranged BPDCN cases. METHODS: Pathology archives from 6 major institutes were queried for cases of BPDCN with 8q24 MYC translocations, identifying two cases...
June 8, 2018: Histopathology
Marine Oberkampf, Camille Guillerey, Juliette Mouriès, Pierre Rosenbaum, Catherine Fayolle, Alexandre Bobard, Ariel Savina, Eric Ogier-Denis, Jost Enninga, Sebastian Amigorena, Claude Leclerc, Gilles Dadaglio
Cross-presentation allows exogenous antigen presentation in association with major histocompatibility complex class I molecules, a process crucial for the priming of CD8+ T-cell responses against viruses and tumors. By contrast to conventional dendritic cells (cDC), which cross-present antigens in the steady state, plasmacytoid dendritic cells (pDC) acquire this ability only after stimulation by Toll-like receptor (TLR) ligands. The intracellular pathways accounting for this functional difference are still unknown...
June 8, 2018: Nature Communications
Fang Yu, Ke Sun, Zhaoming Wang
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare disease with an aggressive clinical course resulting in median survival times of 12 to 14 months.1 It represents approximately 0.8% of primary cutaneous lymphomas.2 According to the World Health Organization Classification of Tumors of Hematopoietic and Lymphoid Tissue, published in 2008, BPDCN is defined as an acute myeloid leukemia related precursor neoplasm, derived from precursors of the plasmacytoid dendritic cell. It is characterized microscopically by its dense monomorphous infiltrates of medium-sized blastoid morphology and expression of CD4 and CD56, as well as the absence of any common myeloid, T-lymphoid, B-lymphoid, and natural killer-lymphoid lineage markers...
May 3, 2018: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Mats W Johansson, Elizabeth A Kelly, Christopher L Nguyen, Nizar N Jarjour, Bruce S Bochner
BACKGROUND: Siglec-8 is present at a high level on human blood eosinophils and low level on blood basophils. Engagement of Siglec-8 on blood eosinophils causes its internalization and results in death. Siglec-8 is a potential therapeutic target in eosinophilic asthma. OBJECTIVES: The aim of this study was to determine Siglec-8 levels on eosinophils and basophils recruited during lung inflammation. METHOD: We analyzed surface Siglec-8 by flow cytometry on cells obtained by bronchoalveolar lavage (BAL) 48 h after segmental lung allergen challenge of human subjects with mild allergic asthma and used confocal microscopy to compare Siglec-8 distribution on BAL and blood eosinophils...
June 7, 2018: International Archives of Allergy and Immunology
Daniela Bosisio, Roberto Ronca, Valentina Salvi, Marco Presta, Silvano Sozzani
Lymph node (LN) expansion during inflammation is essential to establish immune responses and relies on the development of blood and lymph vessels. Human dendritic cells (DCs), subdivided into two main subsets, namely conventional DCs (cDCs) and plasmacytoid DCs (pDCs), are professional antigen presenting cells endowed with the capability to produce soluble mediators regulating inflammation and tissue repair. cDCs support angiogenesis in secondary LNs both directly and indirectly through the secretion of vascular endothelial growth factor-A (VEGF)-A and VEGF-C and the production of several other mediators endowed with angiogenic properties...
June 4, 2018: Current Opinion in Immunology
M Kinoshita-Ise, R Uchida, M Shioiri, Y Ohmori, N Takayama, M Ohyama
No abstract text is available yet for this article.
June 6, 2018: Clinical and Experimental Dermatology
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