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https://www.readbyqxmd.com/read/28109007/retinoic-acid-inducible-gene-i-rig-i-like-receptors-rlrs-in-fish-current-knowledge-and-future-perspectives
#1
REVIEW
Shan Nan Chen, Peng Fei Zou, Pin Nie
Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) are found conservatively present in teleost fish. All three members, RIG-I, MDA5 and LGP2 together with the downstream molecules such as MITA, TRAF3 and TBK1 have been identified in a range of fish species. However, it is a bit unexpected that RIG-I has not been reported in fish of Acanthopterygii, and it would be important to clarify the presence and role of RIG-I gene in a broad range of taxa in Teleostei. RLRs in fish can be induced in vivo and in vitro by viral pathogens as well as synthetic double-stranded RNA (dsRNA), poly(I:C), leading to the production of type I IFNs and the expression of IFN stimulated genes (ISGs)...
January 20, 2017: Immunology
https://www.readbyqxmd.com/read/28090671/lgp2-plays-an-essential-role-in-hcv-infection-induced-interferon-responses
#2
Lei Hei, Jin Zhong
: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) are cytosolic pattern recognition receptors (PRR) that detect non-self RNA and activate downstream interferon (IFN) signaling. One of the RLRs, Laboratory of genetics and physiology 2 (LGP2) was originally thought to be a negative feedback regulator in RIG-I signaling pathway, but growing evidence indicates LGP2 is one co-factor of Melanoma Differentiation-Associated protein 5 (MDA5) in MDA5-mediated IFN signaling activation...
January 16, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28089977/antimelanoma-differentiation-associated-gene-5-antibody-expanding-the-clinical-spectrum-in-north-american-patients-with-dermatomyositis
#3
Siamak Moghadam-Kia, Chester V Oddis, Shinji Sato, Masataka Kuwana, Rohit Aggarwal
OBJECTIVE: To determine the clinical features associated with the antimelanoma differentiation-associated gene 5 antibody (anti-MDA5) in US patients with clinically amyopathic dermatomyositis (CADM) and classic DM. METHODS: Patients with CADM were consecutively selected from the University of Pittsburgh Myositis Database from 1985 to 2013. CADM was defined by a typical DM rash without objective muscle weakness and no or minimal abnormalities of muscle enzymes, electromyography, or muscle biopsy...
January 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/28063952/transcriptome-profiling-of-spleen-provides-insights-into-the-antiviral-mechanism-in-schizothorax-prenanti-after-poly-i-c-challenge
#4
Xiaogang Du, Yunkun Li, Dong Li, Fangliang Lian, Shiyong Yang, Jiayun Wu, Hanmei Liu, Guixian Bu, Fengyan Meng, Xiaohan Cao, Xianyin Zeng, Huaiyu Zhang, Zhiyu Chen
Schizothorax prenanti (S. prenanti) is an important economical cold-water fish species in southwestern China, but it is susceptible to various pathogens infection. In order to clearly elucidate the antiviral mechanism, in this study, we have analyzed the transcriptome of S. prenanti spleen after challenge with the virus mimic, poly (I:C) (pIC), using next generation sequencing technology (RNA-seq). A total of 313 differential expressed genes (DEGs) in spleen at 12 h were obtained after pIC treatment, including 268 significantly up-regulated unigenes (fold change > 2) and 45 significantly down-regulated unigenes (fold change > 2)...
January 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28040421/anti-mda5-dermatomyositis-mimicking-psoriatic-arthritis
#5
Iván Cabezas-Rodríguez, Isla Morante-Bolado, Anahy Brandy-García, Rubén Queiro-Silva, Lourdes Mozo, Francisco Javier Ballina-García
Dermatomyositis causes inflammation and damage of muscle and skin, and sometimes involves internal organs, especially lung parenchyma. Patients with dermatomyositis still represent a diagnostic challenge because of the rarity of this disease and the lack of specificity of some of its cutaneous manifestations. Herein, we describe the case of a patient with dermatomyositis, initially diagnosed as psoriatic arthritis, in which the performance of anti-melanoma differentiation-associated gene 5 (MDA5) antibodies was decisive to establish a definitive diagnosis...
December 28, 2016: Reumatología Clinica
https://www.readbyqxmd.com/read/28031478/trim65-catalized-ubiquitination-is-essential-for-mda5-mediated-antiviral-innate-immunity
#6
Xueting Lang, Tiantian Tang, Tengchuan Jin, Chen Ding, Rongbin Zhou, Wei Jiang
MDA5 plays a critical role in antiviral innate immunity by functioning as a cytoplasmic double-stranded RNA sensor that can activate type I interferon signaling pathways, but the mechanism for the activation of MDA5 is poorly understood. Here, we show that TRIM65 specifically interacts with MDA5 and promotes K63-linked ubiquitination of MDA5 at lysine 743, which is critical for MDA5 oligomerization and activation. Trim65 deficiency abolishes MDA5 agonist or encephalomyocarditis virus (EMCV)-induced interferon regulatory factor 3 (IRF3) activation and type I interferon production but has no effect on retinoic acid-inducible I (RIG-I), Toll-like receptor 3 (TLR3), or cyclic GMP-AMP synthase signaling pathways...
December 28, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28012490/association-of-anti-aminoacyl-transfer-rna-synthetase-antibody-and-anti-melanoma-differentiation-associated-gene-5-antibody-with-the-therapeutic-response-of-polymyositis-dermatomyositis-associated-interstitial-lung-disease
#7
Naomi Yoshida, Masaki Okamoto, Shinjiro Kaieda, Kiminori Fujimoto, Tomohiro Ebata, Morihiro Tajiri, Masayuki Nakamura, Masaki Tominaga, Daisuke Wakasugi, Tomotaka Kawayama, Masataka Kuwana, Tsuneyo Mimori, Hiroaki Ida, Tomoaki Hoshino
BACKGROUND: We attempted to clarify whether the presence of anti-aminoacyl-transfer RNA synthetase antibody (anti-ARS Ab) or anti-melanoma differentiation-associated gene 5 antibody (anti-MDA5 Ab) is associated with the therapeutic response of polymyositis/dermatomyositis-associated interstitial lung disease (PM/DM-ILD). METHODS: We retrospectively investigated 22 patients with PM/DM-ILD (10 positive for anti-ARS Ab and nine positive for anti-MDA5 Ab) for whom antibody analysis of conserved serum was possible...
January 2017: Respiratory Investigation
https://www.readbyqxmd.com/read/28000722/an-ifih1-gene-polymorphism-associated-with-risk-for-autoimmunity-regulates-canonical-antiviral-defence-pathways-in-coxsackievirus-infected-human-pancreatic-islets
#8
Erna Domsgen, Katharina Lind, Lingjia Kong, Michael H Hühn, Omid Rasool, Frank van Kuppeveld, Olle Korsgren, Riitta Lahesmaa, Malin Flodström-Tullberg
The IFIH1 gene encodes the pattern recognition receptor MDA5. A common polymorphism in IFIH1 (rs1990760, A946T) confers increased risk for autoimmune disease, including type 1-diabetes (T1D). Coxsackievirus infections are linked to T1D and cause beta-cell damage in vitro. Here we demonstrate that the rs1990760 polymorphism regulates the interferon (IFN) signature expressed by human pancreatic islets following Coxsackievirus infection. A strong IFN signature was associated with high expression of IFNλ1 and IFNλ2, linking rs1990760 to the expression of type III IFNs...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27928683/specific-autoantibodies-in-dermatomyositis-a-helpful-tool-to-classify-different-clinical-subsets
#9
Giulia Merlo, Andrea Clapasson, Emanuele Cozzani, Luigi Sanna, Giampaola Pesce, Marcello Bagnasco, Martina Burlando, Aurora Parodi
Autoantibodies are important in the diagnosis of dermatomyositis. They can be divided in two different groups: myositis-associated autoantibodies (MAA) prevailing in overlap syndromes, and myositis-specific autoantibodies (MSA), with diagnostic specificity exceeding 90%. Our purpose was to detect retrospectively the prevalence of the most common MSAs in a group of 19 adult DM patients (13 women, 6 men). A severe DM (SDM), with extensive cutaneous and muscular manifestations, dysphagia, and sometimes pneumopathy, was detected in ten cases...
December 7, 2016: Archives of Dermatological Research
https://www.readbyqxmd.com/read/27919567/splicing-factor-proline-glutamine-rich-is-a-novel-autoantigen-of-dermatomyositis-and-associated-with-anti-melanoma-differentiation-associated-gene-5-antibody
#10
Yuji Hosono, Ran Nakashima, Satoshi Serada, Kosaku Murakami, Yoshitaka Imura, Hajime Yoshifuji, Koichiro Ohmura, Tetsuji Naka, Tsuneyo Mimori
OBJECTIVE: Anti-MDA5 antibody positive dermatomyositis (DM) and clinically amyopathic DM (CADM) often develop into rapidly progressive interstitial lung disease, but their pathogenesis remains unclear. We observed that sera from DM/CADM patients immunoprecipitated a common 110 kDa polypeptide. We investigated this autoantigen and its clinical significance. METHODS: Autoantibodies were screened in 333 patients with various connective tissue diseases (CTDs) and 20 healthy controls (HCs) by immunoprecipitation with [(35)S]methionine-labeled HeLa cells...
December 2, 2016: Journal of Autoimmunity
https://www.readbyqxmd.com/read/27914220/modulation-of-the-innate-immune-related-genes-expression-in-h9n2-avian-influenza-virus-infected-chicken-macrophage-like-cells-hd11-in-response-to-escherichia-coli-lps-stimulation
#11
Xuefeng Qi, Caihong Liu, Ruiqiao Li, Huizhu Zhang, Xingang Xu, Jingyu Wang
Macrophages play important roles in mediating virus-induced innate immune responses and are thought to be involved in the pathogenesis of bacterial superinfections. The innate immune response initiated by both low pathogenicity AIV and bacterial superinfection in their avian host is not fully understood. We therefore determine the transcripts of innate immune-related genes following avian H9N2 AIV virus infection and E. coli LPS co-stimulation of avian macrophage-like cell line HD11 cells. More pronounced expression of pro-inflammatory cytokines (IL-6 and IL-1β) as well as the inflammatory chemokines (CXCLi1 and CXCLi2) was observed in virus infected plus LPS treated HD11 cells compared to H9N2 virus solely infected control...
November 17, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27894672/microrna-145-regulates-the-rlr-signaling-pathway-in-miiuy-croaker-after-poly-i-c-stimulation-via-targeting-mda5
#12
Jingjing Han, Yuena Sun, Weihua Song, Tianjun Xu
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that participate in diverse biological processes via degrading the target mRNAs or repressing translation. In this study, the regulation of miRNA to the RLR (RIG-I-like receptor) signaling pathway by degrading the target mRNAs was researched in miiuy croaker. MDA5, a microRNA-145-5p (miR-145-5p) putative target gene, was predicted by bioinformatics, and the target sites from the 3'untranslated region of MDA5 transcripts were confirmed using luciferase reporter assays...
March 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27888997/comprehensive-assessment-of-myositis-specific-autoantibodies-in-polymyositis-dermatomyositis-associated-interstitial-lung-disease
#13
Hironao Hozumi, Tomoyuki Fujisawa, Ran Nakashima, Takeshi Johkoh, Hiromitsu Sumikawa, Akihiro Murakami, Noriyuki Enomoto, Naoki Inui, Yutaro Nakamura, Yuji Hosono, Yoshitaka Imura, Tsuneyo Mimori, Takafumi Suda
OBJECTIVES: Myositis-specific autoantibodies (MSAs) are associated with clinical phenotypes in polymyositis/dermatomyositis (PM/DM). No study has investigated the clinical features based on comprehensive MSA assessment in PM/DM-associated interstitial lung disease (ILD). We aimed to determine the practical significance of MSAs in PM/DM-ILD. METHODS: Sixty consecutive PM/DM-ILD patients were retrospectively analysed. Serum MSAs were comprehensively measured using immunoprecipitation assay...
December 2016: Respiratory Medicine
https://www.readbyqxmd.com/read/27851860/dermatopulmonary-syndrome-associated-with-anti-mda5-antibodies-after-allogeneic-hematopoietic-stem-cell-transplantation
#14
Clémence Lepelletier, Djaouida Bengoufa, Zeltni Lyes, Adèle de Masson, François Chasset, Marie Jachiet, David Michonneau, Marie Robin, Régis Peffault de Latour, Flore Sicre de Fontbrune, Yacine Tandjaoui-Lambiotte, Armand Bensussan, Michel Rybojad, Abdellatif Tazi, Martine Bagot, Gérard Socié, Anne Bergeron, Jean-David Bouaziz
Importance: Chronic graft-vs-host-disease (cGVHD) after allogeneic stem cell transplantation (AHSCT) may resemble autoimmune diseases. Anti-MDA5 (melanoma differentiation-associated gene 5) dermatopulmonary syndrome is a subset of dermatomyositis defined by specific clinical features and detection of anti-MDA5-antibodies in the serum. Objective: To characterize the clinical features of patients who underwent AHSCT and screened positively for anti-MDA5 antibodies...
November 16, 2016: JAMA Dermatology
https://www.readbyqxmd.com/read/27847343/fish-trim32-functions-as-a-critical-antiviral-molecule-against-iridovirus-and-nodavirus
#15
Yepin Yu, Xiaohong Huang, Jiaxin Liu, Jingcheng Zhang, Yin Hu, Ying Yang, Youhua Huang, Qiwei Qin
Tripartite motif-containing 32 (TRIM32) has been demonstrated to pay vital roles in cancer, genetic disorders and antiviral immunity. However, the molecular functions of fish TRIM32 still remained largely unknown. Here, a novel TRIM32 gene from orange spotted grouper (EcTRIM32) was cloned and characterized. EcTRIM32 encoded a 685-aa protein which showed 93%, and 60% identity to large yellow croaker (Larimichthys crocea) and human (Homo sapiens), respectively. Amino acid alignment showed that EcTRIM32 contained a conserved RING-finger domain, a BBOX domain and NHL domain...
November 12, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27846843/strong-inflammatory-responses-and-apoptosis-in-the-oviducts-of-egg-laying-hens-caused-by-genotype-viid-newcastle-disease-virus
#16
Ruiqiao Li, Kangkang Guo, Caihong Liu, Jing Wang, Dan Tan, Xueying Han, Chao Tang, Yanming Zhang, Jingyu Wang
BACKGROUND: Newcastle disease virus (NDV) can cause serious damage to the reproductive tracts of egg-laying hens and leads to egg production and quality reduction. However, the mechanism of severe pathological damage in the oviducts of egg-laying hens after NDV infection has not been fully elucidated. In this study, the correlation between the primary pathological lesions and viral load in the oviducts of egg-laying hens infected with the velogenic genotype VIId NDV strain was evaluated by pathological observation and virus detection...
November 15, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27825821/constitutive-overexpressed-type-i-interferon-induced-downregulation-of-antiviral-activity-in-medaka-fish-oryzias-latipes
#17
Shun Maekawa, Takashi Aoki, Han-Ching Wang
In fish, as well as vertebrates, type I interferons (IFNs) are important cytokines that help to provide innate, antiviral immunity. Although low amounts of IFN are constitutively secreted under normal physiological conditions, long-term and excessive IFN stimulation leads to reduced sensitivity to the IFN signal. This provides a negative feedback mechanism that prevents inappropriate responses and autoimmunity. At present, however, neither IFN desensitization nor the normal physiological role of constitutive IFN are well characterized in fish...
March 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27815200/fish-trim16l-exerts-negative-regulation-on-antiviral-immune-response-against-grouper-iridoviruses
#18
Yepin Yu, Xiaohong Huang, Jingcheng Zhang, Jiaxi Liu, Yin Hu, Ying Yang, Jia Cai, Youhua Huang, Qiwei Qin
Tripartite motif 16 (TRIM16), has been demonstrated to act as a tumor suppressor through affecting cell proliferation and migration or tumorigenicity in carcigenesis. However, the roles of TRIM16 in immune response were unknown up to now. Here, we cloned a TRIM16-like gene (TRIM16L) from orange spotted grouper (EcTRIM16L) and investigated its roles in response to virus infection. EcTRIM16L encoded a 478 amino acid peptide which showed 72% and 29% identity to large yellow croaker (Larimichthys crocea) and human (Homo sapiens), respectively...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27793594/cross-regulation-of-two-type-i-interferon-signaling-pathways-in-plasmacytoid-dendritic-cells-controls-anti-malaria-immunity-and-host-mortality
#19
Xiao Yu, Baowei Cai, Mingjun Wang, Peng Tan, Xilai Ding, Jian Wu, Jian Li, Qingtian Li, Pinghua Liu, Changsheng Xing, Helen Y Wang, Xin-Zhuan Su, Rong-Fu Wang
Type I interferon (IFN) is critical for controlling pathogen infection; however, its regulatory mechanisms in plasmacytoid cells (pDCs) still remain unclear. Here, we have shown that nucleic acid sensors cGAS-, STING-, MDA5-, MAVS-, or transcription factor IRF3-deficient mice produced high amounts of type I IFN-α and IFN-β (IFN-α/β) in the serum and were resistant to lethal plasmodium yoelii YM infection. Robust IFN-α/β production was abolished when gene encoding nucleic acid sensor TLR7, signaling adaptor MyD88, or transcription factor IRF7 was ablated or pDCs were depleted...
November 15, 2016: Immunity
https://www.readbyqxmd.com/read/27761751/myositis-specific-autoantibodies-and-their-association-with-malignancy-in-italian-patients-with-polymyositis-and-dermatomyositis
#20
Angela Ceribelli, Natasa Isailovic, Maria De Santis, Elena Generali, Micaela Fredi, Ilaria Cavazzana, Franco Franceschini, Luca Cantarini, Minoru Satoh, Carlo Selmi
This study aims to characterize myositis-specific antibodies in a well-defined cohort of patients with idiopathic inflammatory myopathy and to determine their association with cancer. Sera from 40 patients with polymyositis, dermatomyositis, and controls were tested by protein and RNA immunoprecipitation to detect autoantibodies, and immunoprecipitation-Western blot was used for anti-MJ/NXP-2, anti-MDA5, and anti-TIF1γ/α identification. Medical records were re-evaluated with specific focus on cancer. Anti-MJ/NXP-2 and anti-TIF1γ/α were the most common antibodies in dermatomyositis...
October 20, 2016: Clinical Rheumatology
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