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https://www.readbyqxmd.com/read/28435073/evaluation-of-a-rapid-detection-for-coxsackievirus-b3-using-one-step-reverse-transcription-loop-mediated-isothermal-amplification-rt-lamp
#1
A Monazah, M Zeinoddini, A R Saeeidinia
Coxsackievirus B3 (CVB3) is a member of the genus Enterovirus within the family Picornaviridae and is an important pathogen of viral myocarditis, which accounts for more than 50% viral myocarditis cases. VP1 is major capsid protein that this region has a low homology in both amino acid and nucleotide sequences among Enteroviruses. Therefore we have chosen this region for designed a set of RT-LAMP primers for CVB3 detection. For this the total RNA was extracted from 24-h post infected-HeLa cells with complete cytopathic effect (CPE), and applied to a one-step reverse transcription loop-mediated isothermal amplification reaction (RT-LAMP) using CVB3-specific primers...
April 20, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28435072/development-of-a-pseudovirus-based-assay-for-measuring-neutralizing-antibodies-against-coxsackievirus-b5
#2
Pan Chen, Xing Wu, Yao Su, Xiaotian Hao, Qunying Mao, Zhenglun Liang
Coxsackievirus B5 (CV-B5), an important Coxsackie B virus from genus Enteroviruse within the family Picornaviridae, has also been isolated from Hand, Foot, and Mouth Disease (HFMD) patients, and often associated with neurological manifestations. In this study, we found out that Coxsackievirus B3 (CV-B3) replicon RNA could be encapsidated with CV-B5 capsid to assemble infectious CV-B5 pseudovirus. We then utilized this single round infection system of CV-B5 to develop a neutralizing antibody quantification assay...
April 20, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28427827/antiviral-screen-identifies-ev71-inhibitors-and-reveals-camptothecin-target-dna-topoisomerase-1-as-a-novel-ev71-host-factor
#3
Kan Xing Wu, Justin Jang-Hann Chu
Enterovirus 71 (EV71) is one of the causative agents of hand, foot and mouth disease (HFMD) associated with severe neurological disease. EV71's pathogenesis remains poorly understood and the lack of approved antiviral has led to its emergence as a clinically important neurotropic virus. The goals of this study were to: (i) identify novel anti-EV71 compounds that may serve as lead molecules for therapeutics; and (ii) investigate their targets in downstream studies. We screened a 502-compound library of highly purified natural products for anti-EV71 activities in a cell-based immunofluorescence assay that were then confirmed in viral plaque reduction assays...
April 17, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28424289/disruption-of-mda5-mediated-innate-immune-responses-by-the-3c-proteins-of-coxsackievirus-a16-coxsackievirus-a6-and-enterovirus-d68
#4
Yajuan Rui, Jiaming Su, Hong Wang, Junliang Chang, Shaohua Wang, Wenwen Zheng, Yong Cai, Wei Wei, James T Gordy, Richard Markham, Wei Kong, Wenyan Zhang, Xiao-Fang Yu
Coxsackievirus A16 (CV-A16), A6 (CV-A6), and enterovirus D68 (EV-D68) belong to the Picornaviridae family and are major causes of hand, foot, and mouth disease (HFMD) and pediatric respiratory disease worldwide. The biological characteristics of these viruses, especially their interplay with the host innate immune system, have not been well investigated. In this study, we discovered that the 3C(pro) proteins from CV-A16, CV-A6, and EV-D68 bind MDA5 and inhibit its interaction with MAVS. Consequently, MDA5-triggered type I IFN signaling in the RLR pathway was blocked by CV-A16, CV-A6, and EV-D68 3C(pro) Furthermore, CV-A16, CV-A6, and EV-D68 3C(pro) all cleave TAK1, resulting in inhibition of NF-κB activation, a host response also critical for toll-like receptor (TLR) mediated signaling...
April 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28424287/protective-efficacy-of-formaldehyde-inactivated-whole-virus-vaccine-and-antivirals-in-a-murine-model-of-coxsackievirus-a10-infection
#5
Zhenjie Zhang, Zhaopeng Dong, Juan Li, Michael J Carr, Dongming Zhuang, Jianxing Wang, Yawei Zhang, Shujun Ding, Yigang Tong, Dong Li, Weifeng Shi
Coxsackievirus A10 (CVA10) is one of the major pathogens associated with hand, foot and mouth disease (HFMD). CVA10 infection can cause herpangina and viral pneumonia, which can be complicated by severe neurological sequelae. Morbidity and mortality of CVA10-associated HFMD has been increasing in recent years, particularly in the pan-Pacific region. There are limited studies however on the pathogenesis and immunology of CVA10-associated HFMD infections, and few antiviral drugs or vaccines have been reported...
April 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28413820/coxsackievirus-b5-associated-with-hand-foot-mouth-disease-in-a-healthy-adult
#6
Anthony R Andreoni, Andrea S Colton
No abstract text is available yet for this article.
March 2017: JAAD Case Reports
https://www.readbyqxmd.com/read/28411509/direct-acting-antivirals-and-host-targeting-strategies-to-combat-enterovirus-infections
#7
REVIEW
Lisa Bauer, Heyrhyoung Lyoo, Hilde M van der Schaar, Jeroen Rpm Strating, Frank Jm van Kuppeveld
Enteroviruses (e.g., poliovirus, enterovirus-A71, coxsackievirus, enterovirus-D68, rhinovirus) include many human pathogens causative of various mild and more severe diseases, especially in young children. Unfortunately, antiviral drugs to treat enterovirus infections have not been approved yet. Over the past decades, several direct-acting inhibitors have been developed, including capsid binders, which block virus entry, and inhibitors of viral enzymes required for genome replication. Capsid binders and protease inhibitors have been clinically evaluated, but failed due to limited efficacy or toxicity issues...
April 12, 2017: Current Opinion in Virology
https://www.readbyqxmd.com/read/28408118/a-novel-combined-vaccine-based-on-monochimeric-vlp-co-displaying-multiple-conserved-epitopes-against-enterovirus-71-and-varicella-zoster-virus
#8
Yangtao Wu, Rui Zhu, Longfa Xu, Yongchao Li, Shuxuan Li, Hai Yu, Shaowei Li, Hua Zhu, Tong Cheng, Ningshao Xia
Chicken pox and hand, foot and mouth disease (HFMD) are two major infectious diseases that mainly affect infants and children, causing significant morbidity annually. Varicella-zoster virus (VZV) and enterovirus 71 (EV71), respectively, are the principal epidemic pathogens causing these two diseases. To investigate the possibility of developing a novel combined vaccine to prevent chicken pox and HFMD, we constructed three chimeric virus-like particles (VLPs) (termed HBc-V/1/2, HBc-2/V/1 and HBc-1/2/V) based on the hepatitis B core antigen (HBc) carrier that display epitopes derived from VZV-gE, EV71-VP1, and EV71-VP2 in a varied tandem manner...
May 9, 2017: Vaccine
https://www.readbyqxmd.com/read/28407709/synergistic-antitumor-effect-of-ing4-pten-double-tumor-suppressors-mediated-by-adenovirus-modified-with-rgd-on-glioma
#9
Yaodong Zhao, Jiarong Wang, Jicheng Yang, Jingcheng Miao
BACKGROUND: Gene therapy is regarded as a new and promising therapeutic modality for cancers, and adenovirus is one of the most frequently used vectors. However, because of low or absent coxsackievirus and adenovirus receptor levels on the surface of many kinds of tumor cells, the efficiency of adenovirus infection of target tumor cells may be low. Meanwhile, gene therapy by a single vector carrying two or more anti-oncogenes can improve treatment effects and reduce side effects from vectors...
April 12, 2017: Journal of Neurosurgical Sciences
https://www.readbyqxmd.com/read/28384999/pathogenesis-of-coxsackievirus-b2-in-mice-characterisation-of-clinical-isolates-of-the-coxsackievirus-b2-from-patients-with-myocarditis-and-aseptic-meningitis-in-korea
#10
JiYoung Hong, BungHak Kang, SangGu Yeo, Youngmee Jee, Jae-Hak Park
Group B coxsackieviruses (CVBs) are a group of common human pathogens causing various clinical symptoms. Although the virology of CVB is well known, there is limited information on viral pathogenesis and the relationship between clinical symptoms and viral phenotype, particularly for CVB type 2 (CVB2). In this study, two CVB2 strains were isolated: CB2/04/279 from a stool of an acute myocarditis patient with heart failure; and CB2/04/243 from an aseptic meningitis patient in 2004 in Korea. A high degree of homology was observed between the CB2/04/279 and CB2/04/243 full genome sequences...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28381577/understanding-the-mechanism-of-the-broad-spectrum-antiviral-favipiravir-t-705-key-role-of-the-f1-motif-of-the-viral-polymerase
#11
Rana Abdelnabi, Ana Theresa Silveira de Morais, Pieter Leyssen, Isabelle Imbert, Stéphanie Beaucourt, Hervé Blanc, Mathy Froeyen, Marco Vignuzzi, Bruno Canard, Johan Neyts, Leen Delang
Favipiravir (T-705) is a broad-spectrum antiviral agent that has been approved in Japan for treatment of influenza virus infections. T-705 also inhibits the replication of various RNA viruses including chikungunya virus (CHIKV). We demonstrated earlier that the K291R mutation in the F1 motif of the RNA-dependent RNA polymerase (RdRp) of CHIKV is responsible for low-level resistance to T-705. Interestingly, this lysine is highly conserved in the RdRp of positive-sense single-stranded RNA [+ssRNA] viruses. To obtain insights into the unique broad-spectrum antiviral activity of T-705, we explored the role of this lysine using another +ssRNA virus, namely Coxsackievirus B3 (CVB3)...
April 5, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28381405/coxsackievirus-a21-synergizes-with-checkpoint-inhibitors
#12
(no author information available yet)
Treatment with a combination of a proprietary formulation of coxsackievirus and either an anti-CTLA-4 or anti-PD-1 checkpoint inhibitor yielded a higher response rate in phase I testing for melanoma than any of these drugs given on their own. Because the viral therapy adds little toxicity, it might prove an effective part of a dual regimen, according to interim trial data presented at the American Association for Cancer Research Annual Meeting 2017.
April 5, 2017: Cancer Discovery
https://www.readbyqxmd.com/read/28368491/viral-pathogen-detection-by-metagenomics-and-pan-viral-group-pcr-in-children-with-pneumonia-lacking-identifiable-etiology
#13
Robert Schlaberg, Krista Queen, Keith Simmon, Keith Tardif, Chris Stockmann, Steven Flygare, Brett Kennedy, Karl Voelkerding, Anna Bramley, Jing Zhang, Karen Eilbeck, Mark Yandell, Seema Jain, Andrew T Pavia, Suxiang Tong, Krow Ampofo
Background: Community-acquired pneumonia (CAP) is a leading cause of pediatric hospitalization. Pathogen identification fails in ~20% of children but is critical for optimal treatment and prevention of hospital-acquired infections. We used two broad-spectrum detection strategies to identify pathogens in test-negative children with CAP and asymptomatic controls. Methods: Nasopharyngeal/oropharyngeal (NP/OP) swabs from 70 children <5 years with CAP of unknown etiology and 90 asymptomatic controls were tested by next-generation sequencing (RNA-seq) and pan viral group (PVG) PCR for 19 viral families...
March 25, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28367766/epidemics-and-aetiology-of-hand-foot-and-mouth-disease-in-xiamen-china-from-2008-to-2015
#14
S Z He, M Y Chen, X R Xu, Q Yan, J J Niu, W H Wu, X S Su, S X Ge, S Y Zhang, N S Xia
Over the past 8 years, human enteroviruses (HEVs) have caused 27 227 cases of hand, foot and mouth disease (HFMD) in Xiamen, including 99 severe cases and six deaths. We aimed to explore the molecular epidemiology of HFMD in Xiamen to inform the development of diagnostic assays, vaccines and other interventions. From January 2009 to September 2015, 5866 samples from sentinel hospitals were tested using nested reverse transcription PCR that targeted the HEV 5' untranslated region and viral protein 1 region. Of these samples, 4290 were tested positive for HEV and the amplicons were sequenced and genotyped...
April 3, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28361043/cleavage-of-grb2-associated-binding-protein-2-by-viral-proteinase-2a-during-coxsackievirus-infection
#15
Haoyu Deng, Gabriel Fung, Ye Qiu, Chen Wang, Jingchun Zhang, Zheng-Gen Jin, Honglin Luo
Coxsackievirus type B3 (CV-B3), an enterovirus associated with the pathogenesis of several human diseases, subverts, or employs the host intracellular signaling pathways to support effective viral infection. We have previously demonstrated that Grb2-associated binding protein 1 (GAB1), a signaling adaptor protein that serves as a platform for intracellular signaling assembly and transduction, is cleaved upon CV-B3 infection, resulting in a gain-of-pro-viral-function via the modification of GAB1-mediated ERK1/2 pathway...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28352641/cardiac-function-remains-impaired-despite-reversible-cardiac-remodeling-after-acute-experimental-viral-myocarditis
#16
Peter Moritz Becher, Frauke Gotzhein, Karin Klingel, Felicitas Escher, Stefan Blankenberg, Dirk Westermann, Diana Lindner
Background. Infection with Coxsackievirus B3 induces myocarditis. We aimed to compare the acute and chronic phases of viral myocarditis to identify the immediate effects of cardiac inflammation as well as the long-term effects after resolved inflammation on cardiac fibrosis and consequently on cardiac function. Material and Methods. We infected C57BL/6J mice with Coxsackievirus B3 and determined the hemodynamic function 7 as well as 28 days after infection. Subsequently, we analyzed viral burden and viral replication in the cardiac tissue as well as the expression of cytokines and matrix proteins...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28343845/curcumin-inhibits-zika-and-chikungunya-virus-infection-by-inhibiting-cell-binding
#17
Bryan C Mounce, Teresa Cesaro, Lucia Carrau, Thomas Vallet, Marco Vignuzzi
Several compounds extracted from spices and herbs exhibit antiviral effects in vitro, suggesting potential pharmacological uses. Curcumin, a component of turmeric, has been used as a food additive and herbal supplement due to its potential medicinal properties. Previously, curcumin exhibited antiviral properties against several viruses, including dengue virus and hepatitis C virus, among others. Here, we describe the antiviral effect of curcumin on Zika and chikungunya viruses, two mosquito-borne outbreak viruses...
June 2017: Antiviral Research
https://www.readbyqxmd.com/read/28341822/berberine-restricts-coxsackievirus-b-type-3-replication-via-inhibition-of-c-jun-n-terminal-kinase-jnk-and-p38-mapk-activation-in-vitro
#18
Qian Dai, Di Zhang, Hua Yu, Wei Xie, Rong Xin, Lei Wang, Xiaohui Xu, Xiaomei He, Junzhi Xiong, Halei Sheng, Le Zhang, Kebin Zhang, Xiaomei Hu
BACKGROUND At present, the treatment of coxsackievirus-induced myocarditis remains difficult. Berberine (BBR), an isoquinoline alkaloid isolated from traditional medicine herbs, exhibits significant anti-viral efficacy against various viruses. However, the underlying mechanism by which BBR controls CVB3 infection has not yet been reported. The purpose of this study was to investigate the anti-viral efficacy of BBR against CVB3 infection and its mechanism. MATERIAL AND METHODS In our experiments, the protein levels of VP1 and MAPKs signal pathway were measured by Western blot...
March 25, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28332617/molecular-epidemiology-of-acute-hemorrhagic-conjunctivitis-caused-by-coxsackie-a-type-24-variant-in-china-2004-2014
#19
Li Zhang, Na Zhao, Xiaodan Huang, Xiuming Jin, Xingyi Geng, Ta-Chien Chan, Shelan Liu
To understand control interventions, the molecular epidemiology of acute hemorrhagic conjunctivitis (AHC) was investigated from 2004 to 2014.A total of 613,485 AHC cases (annualized cases 55,771) with two deaths were included. Our findings showed that AHC was reported in all provinces, predominantly in Southern and Eastern China. The incidence rates were highest in 2007 (5.65/100,000) and 2010 (21.78/100,000) respectively. A clear seasonal pattern was identified with a peak from August to October. AHC cases occurred in all age groups; however, five to 14 years was the predominant group [23...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28280379/amylmetacresol-2-4-dichlorobenzyl-alcohol-hexylresorcinol-or-carrageenan-lozenges-as-active-treatments-for-sore-throat
#20
Martina Morokutti-Kurz, Christine Graf, Eva Prieschl-Grassauer
Up to 80% of sore throats are caused by viruses. Several over the counter products are available which provide symptomatic, not causal relief. For such lozenges, containing the antiseptics and local anesthetics amylmetacresol (AMC) and 2,4-dichlorobenzyl alcohol (DCBA) or hexylresorcinol (HR), recently an additional virucidal effect was published. Therefore, we tested a set of Strepsils(®) lozenges, containing either HR (Max [#2]) or AMC/DCBA (Original [#3], Extra Strong [#4], Warm [#5], Orange and Vitamin C [#6], Sugar free Lemon [#7], Children/Strawberry [#8] and Soothing Honey and Lemon [#9]) for their antiviral efficiency against representatives of respiratory viruses known to cause sore throat: human rhinovirus (HRV) 1a, HRV8, influenza virus A H1N1n, Coxsackievirus A10, and human coronavirus (hCoV) OC43...
2017: International Journal of General Medicine
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