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https://www.readbyqxmd.com/read/28239980/rational-design-of-mini-size-carbon-nitride-nanosheets-with-double-excitation-and-ph-dependent-fluorescence-behaviors-for-two-photon-cell-imaging
#1
Liu Jinhua, DuoDuo Zhao, Lin Li, Minrui Weng, Chengwu Zhang, Shiyu Zhang, Jixin Zhu, Yong Feng, Kaimin Shih, Wei Huang
Synthesis of mini-size carbon nitride nanosheets (CNNSs) by using traditional ultrasonic method still remains a great challenge. Herein, Size-tunable and uniform mini-size CNNSs are synthesized by hydrothermal carbonization of the single polyethyleneimine (PEI) precursor.The as-obtained mini-size CNNSs possesses uniform size, good hydrophilicity, abundant nitrogen active sites, and not only exhibit double excitation- and pH-depending fluorescence behaviors, but two-photon exitation fluorescence. The resulting CNNSs display relatively low toxicity and can be efficiently delivered into live cells for two-photon fluorescence image, further confirm that the obtained CNNSs can potentially be used as fluorescence probes in biochemical applications...
February 26, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28239886/fibrocartilaginous-mesenchymoma-of-bone-a-single-institution-experience-with-molecular-investigations-and-a-review-of-the-literature
#2
M Gambarotti, A Righi, D Vanel, S Cocchi, S Benini, F M Elli, G Mantovani, P Ruggieri, S Boriani, D Donati, M Sbaraglia, A P Dei Tos, P Picci
AIMS: Fibrocartilaginous mesenchymoma is a rare intraosseous lesion with a total of 25 cases described in the literature. This study describes the clinical, radiological, and histological features of eight new cases of fibrocartilaginous mesenchymoma collected at a single institution between 1982 and 2016. The presence of GNAS and IDH1/2 mutations and MDM2 amplification was explored to evaluate possible links between fibrocartilaginous mesenchymoma, fibrous dysplasia, dedifferentiated chondrosarcoma, and low-grade osteosarcoma...
February 26, 2017: Histopathology
https://www.readbyqxmd.com/read/28239705/chemical-communication-and-dynamics-of-droplet-emulsions-in-networks-of-belousov-zhabotinsky-micro-oscillators-produced-by-microfluidics
#3
Kristian Torbensen, Federico Rossi, Sandra Ristori, Ali Abou-Hassan
Chemical communication leading to synchronization and collective behaviour of dynamic elements, such as cell colonies, is a widespread phenomenon with biological, physical and chemical importance. Such synchronization between elements proceeds via chemical communication by emmision, interdiffusion and reception of specific messenger molecules. On a lab scale, these phenomena can be modeled by encapsulating an oscillating chemical reaction, which serves as a signal (information) sender/receiver element, inside microcompartments such as droplet emulsions, liposomes and polymersomes...
February 27, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28239692/high-throughput-screening-of-microchip-synthesized-genes-in-programmable-double-emulsion-droplets
#4
H F Chan, S Ma, J Tian, K W Leong
The rapid advances in synthetic biology and biotechnology are increasingly demanding high-throughput screening technology, such as screening of the functionalities of synthetic genes for optimization of protein expression. Compartmentalization of single cells in water-in-oil (W/O) emulsion droplets allows screening of a vast number of individualized assays, and recent advances in automated microfluidic devices further help realize the potential of droplet technology for high-throughput screening. However these single-emulsion droplets are incompatible with aqueous phase analysis and the inner droplet environment cannot easily communicate with the external phase...
February 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28239691/wood-combustion-particles-induce-adverse-effects-to-normal-and-diseased-airway-epithelia
#5
Manuel Krapf, Lisa Künzi, Sandrine Allenbach, Emily A Bruns, Ilaria Gavarini, Imad El-Haddad, Jay G Slowik, André S H Prévôt, Luka Drinovec, Griša Močnik, Lutz Dümbgen, Matthias Salathe, Nathalie Baumlin, Constantinos Sioutas, Urs Baltensperger, Josef Dommen, Marianne Geiser
Residential wood burning is a major source of poorly characterized, deleterious particulate matter, whose composition and toxicity may vary with wood type, burning condition and photochemical age. The causative link between ambient wood particle constituents and observed adverse health effects is currently lacking. Here we investigate the relationship between chemical properties of primary and atmospherically aged wood combustion particles and acute toxicity in human airway epithelial cells. Emissions from a log wood burner were diluted and injected into a smog chamber for photochemical aging...
February 27, 2017: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/28239644/hla-dq-%C3%AE-1-alleles-associated-with-epstein-barr-virus-ebv-infectivity-and-ebv-gp42-binding-to-cells
#6
Qingxue Li, Wei Bu, Erin Gabriel, Fiona Aguilar, Yo Hoshino, Hiroko Miyadera, Christoph Hess, Ronald L Hornung, Amitava Roy, Jeffrey I Cohen
Epstein-Barr virus (EBV) infects B cells and ~95% of adults are infected. EBV glycoprotein gp42 is essential for entry of virus into B cells. EBV gp42 binds to the β1 chain of HLA-DQ, -DR, and -DP on B cells, and uses these molecules for infection. To investigate if certain HLA-DQ alleles are associated with EBV seronegativity, we recruited ~3,300 healthy adult blood donors, identified 106 EBV-seronegative individuals, and randomly selected a control group of EBV-seropositive donors from the donor pool. A larger than expected proportion of EBV-seronegative subjects were HLA-DQ β1 *04/*05 and *06/*06, and to a lesser extent, *02/*03, compared with the control group, while a larger than expected portion of EBV-seropositive persons were HLA-DQ β1 *02/*02...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28239620/analysis-of-phagosomal-antigen-degradation-by-flow-organellocytometry
#7
Eik Hoffmann, Anne-Marie Pauwels, Andrés Alloatti, Fiorella Kotsias, Sebastian Amigorena
Professional phagocytes internalize self and non-self particles by phagocytosis to initiate innate immune responses. After internalization, the formed phagosome matures through fusion and fission events with endosomes and lysosomes to obtain a more acidic, oxidative and hydrolytic environment for the degradation of its cargo. Interestingly, phagosome maturation kinetics differ between cell types and cell activation states. This protocol allows to quantify phagosome maturation kinetics on a single organelle level in different types of phagocytes using flow cytometry...
November 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28239471/gene-expression-markers-of-tumor-infiltrating-leukocytes
#8
Patrick Danaher, Sarah Warren, Lucas Dennis, Leonard D'Amico, Andrew White, Mary L Disis, Melissa A Geller, Kunle Odunsi, Joseph Beechem, Steven P Fling
BACKGROUND: Assays of the abundance of immune cell populations in the tumor microenvironment promise to inform immune oncology research and the choice of immunotherapy for individual patients. We propose to measure the intratumoral abundance of various immune cell populations with gene expression. In contrast to IHC and flow cytometry, gene expression assays yield high information content from a clinically practical workflow. Previous studies of gene expression in purified immune cells have reported hundreds of genes showing enrichment in a single cell type, but the utility of these genes in tumor samples is unknown...
2017: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/28239416/biodegradation-of-alkaline-lignin-by-bacillus-ligniniphilus-l1
#9
Daochen Zhu, Peipei Zhang, Changxiao Xie, Weimin Zhang, Jianzhong Sun, Wei-Jun Qian, Bin Yang
BACKGROUND: Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorganisms able to decompose it or degrade it. Fungi are recognized as the most widely used microbes for lignin degradation. However, bacteria have also been known to be able to utilize lignin as a carbon or energy source. Bacillus ligniniphilus L1 was selected in this study due to its capability to utilize alkaline lignin as a single carbon or energy source and its excellent ability to survive in extreme environments...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28239411/reliable-measurement-of-e-coli-single-cell-fluorescence-distribution-using-a-standard-microscope-set-up
#10
Marilisa Cortesi, Lucia Bandiera, Alice Pasini, Alessandro Bevilacqua, Alessandro Gherardi, Simone Furini, Emanuele Giordano
BACKGROUND: Quantifying gene expression at single cell level is fundamental for the complete characterization of synthetic gene circuits, due to the significant impact of noise and inter-cellular variability on the system's functionality. Commercial set-ups that allow the acquisition of fluorescent signal at single cell level (flow cytometers or quantitative microscopes) are expensive apparatuses that are hardly affordable by small laboratories. METHODS: A protocol that makes a standard optical microscope able to acquire quantitative, single cell, fluorescent data from a bacterial population transformed with synthetic gene circuitry is presented...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28238916/antiresorptive-and-anti-angiogenetic-octacalcium-phosphate-functionalized-with-bisphosphonates-an-in-vitro-tri-culture-study
#11
Lucia Forte, Paola Torricelli, Elisa Boanini, Massimo Gazzano, Milena Fini, Adriana Bigi
: Development of new materials for the local administration of bisphosphonates (BPs) is aimed to avoid the negative side effects of prolonged systemic use of these potent drugs. In this work, we synthesized octacalcium phosphate (OCP) in the presence of two potent BPs and obtained a single crystalline phase up to a zoledronate and alendronate content of 3.5 wt% and 5.2 wt%, respectively. Both BPs provoke minor structural modifications and a reduction of the crystal dimensions of OCP, which suggests a preferential interaction of the BPs with the structure of the calcium phosphate...
February 23, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28238890/time-lapse-imaging-of-p65-and-i%C3%AE%C2%BAb%C3%AE-translocation-kinetics-following-ca-2-induced-neuronal-injury-reveals-biphasic-translocation-kinetics-in-surviving-neurons
#12
Robert Schwamborn, Heiko Düssmann, Hans-Georg König, Jochen H M Prehn
The transcription factor nuclear factor-κB (NF-κB) regulates neuronal differentiation, plasticity and survival. It is well established that excitatory neurotransmitters such as glutamate control NF-κB activity. Glutamate receptor overactivation is also involved in ischemic- and seizure-induced neuronal injury and neurodegeneration. However, little is known at the single cell-level how NF-κB signaling relates to neuronal survival during excitotoxic injury. We found that silencing of p65/NF-κB delayed N-methyl-d-aspartate (NMDA)-induced excitotoxic injury in hippocampal neurons, suggesting a functional role of p65 in excitotoxicity...
February 23, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28238813/genomewide-bisulfite-sequencing-reveals-the-origin-and-time-dependent-fragmentation-of-urinary-cfdna
#13
Timothy H T Cheng, Peiyong Jiang, Jacqueline C W Tam, Xiao Sun, Wing-Shan Lee, Stephanie C Y Yu, Jeremy Y C Teoh, Peter K F Chiu, Chi-Fai Ng, Kai-Ming Chow, Cheuk-Chun Szeto, K C Allen Chan, Rossa W K Chiu, Y M Dennis Lo
Urinary cell-free (cf) DNA holds great potential as a completely noninvasive form of liquid biopsy. Knowledge of the composition of cfDNA by tissue of origin is useful for guiding its clinical uses. We conducted a global survey of urinary cfDNA composition using genomewide bisulfite sequencing. While previous studies focused on detecting cfDNA from a single source at a time, genomewide tissue specific methylation signatures allow us to simultaneously deduce the proportional contribution from each contributing tissue...
February 23, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28238623/salmonella-enterica-remodels-the-host-cell-endosomal-system-for-efficient-intravacuolar-nutrition
#14
Viktoria Liss, A Leoni Swart, Alexander Kehl, Natascha Hermanns, Yuying Zhang, Deepak Chikkaballi, Nathalie Böhles, Jörg Deiwick, Michael Hensel
Salmonella enterica is a facultative intracellular pathogen that survives and proliferates in the Salmonella-containing vacuole (SCV), yet how these vacuolar bacteria acquire nutrition remains to be determined. Intracellular Salmonella convert the host endosomal system into an extensive network of interconnected tubular vesicles, of which Salmonella-induced filaments (SIFs) are the most prominent. We found that membranes and lumen of SIFs and SCVs form a continuum, giving vacuolar Salmonella access to various types of endocytosed material...
February 21, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28238563/the-development-and-use-of-vascular-targeted-therapy-in-ovarian-cancer
#15
REVIEW
Dana M Chase, David J Chaplin, Bradley J Monk
Combination vascular-targeted therapies (VTTs) represent a promising approach for patients with platinum-resistant recurrent ovarian cancer (OC). VTTs include two mechanistically distinct classes of agents: anti-angiogenic agents (AAs) and vascular-disrupting agents (VDAs). AAs suppress growth of new tumor vasculature through inhibition of vascular endothelial growth factor (VEGF) and other pro-angiogenic molecules. Bevacizumab, a monoclonal antibody that binds to VEGF, has improved progression-free survival (PFS) when given with chemotherapy in patients with advanced OC...
February 23, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28238546/regulation-of-thalamic-and-cortical-network-synchrony-by-scn8a
#16
Christopher D Makinson, Brian S Tanaka, Jordan M Sorokin, Jennifer C Wong, Catherine A Christian, Alan L Goldin, Andrew Escayg, John R Huguenard
Voltage-gated sodium channel (VGSC) mutations cause severe epilepsies marked by intermittent, pathological hypersynchronous brain states. Here we present two mechanisms that help to explain how mutations in one VGSC gene, Scn8a, contribute to two distinct seizure phenotypes: (1) hypoexcitation of cortical circuits leading to convulsive seizure resistance, and (2) hyperexcitation of thalamocortical circuits leading to non-convulsive absence epilepsy. We found that loss of Scn8a leads to altered RT cell intrinsic excitability and a failure in recurrent RT synaptic inhibition...
February 18, 2017: Neuron
https://www.readbyqxmd.com/read/28238490/composition-of-single-step-media-used-for-human-embryo-culture
#17
Dean E Morbeck, Nikola A Baumann, Devin Oglesbee
OBJECTIVE: To determine compositions of commercial single-step culture media and test with a murine model whether differences in composition are biologically relevant. DESIGN: Experimental laboratory study. SETTING: University-based laboratory. ANIMAL(S): Inbred female mice were superovulated and mated with outbred male mice. INTERVENTION(S): Amino acid, organic acid, and ions content were determined for single-step culture media: CSC, Global, G-TL, and 1-Step...
February 23, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28238414/histological-features-of-bone-marrow-in-paediatric-patients-during-the-asymptomatic-phase-of-early-stage-black-african-sickle-cell-anaemia
#18
Alessandro Mauriello, Erica Giacobbi, Andrea Saggini, Antonella Isgrò, Simone Facchetti, Lucia Anemona
Bone marrow histological features of sickle cell anaemia (SCA) patients during early stages and in the asymptomatic phase of the disease appear an interesting area of study, representing early-stage consequences of SCA with a close relation to its pathophysiology. Unfortunately, this field of research has never been specifically addressed before. Bone marrow biopsies from 26 consecutive Black African SCA patients (M:F=1.6:1; age 2-17 years), free of clinical signs of chronic bone marrow damage, with no recent history of symptomatic vaso-occlusive episodes, and waiting for haematopoietic stem cell transplantation (HSCT), underwent morphological, immunohistochemical and electron microscopy evaluation...
February 23, 2017: Pathology
https://www.readbyqxmd.com/read/28238159/analyses-of-pea-necrotic-yellow-dwarf-virus-encoded-proteins
#19
Björn Krenz, Ingrid Schießl, Eva Greiner, Susanna Krapp
Pea necrotic yellow dwarf virus (PNYDV) is a multipartite, circular, single-stranded DNA plant virus. PNYDV encodes eight proteins and the function of three of which remains unknown-U1, U2, and U4. PNYDV proteins cellular localization was analyzed by GFP tagging and bimolecular fluorescence complementation (BiFC) studies. The interactions of all eight PNYDV proteins were tested pairwise in planta (36 combinations in total). Seven interactions were identified and two (M-Rep with CP and MP with U4) were characterized further...
February 25, 2017: Virus Genes
https://www.readbyqxmd.com/read/28238136/antibody-conjugated-single-quantum-dot-tracking-of-membrane-neurotransmitter-transporters-in-primary-neuronal-cultures
#20
Danielle M Bailey, Oleg Kovtun, Sandra J Rosenthal
Single particle tracking (SPT) experiments have provided the scientific community with invaluable single-molecule information about the dynamic regulation of individual receptors, transporters, kinases, lipids, and molecular motors. SPT is an alternative to ensemble averaging approaches, where heterogeneous modes of motion might be lost. Quantum dots (QDs) are excellent probes for SPT experiments due to their photostability, high brightness, and size-dependent, narrow emission spectra. In a typical QD-based SPT experiment, QDs are bound to the target of interest and imaged for seconds to minutes via fluorescence video microscopy...
2017: Methods in Molecular Biology
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