keyword
MENU ▼
Read by QxMD icon Read
search

microRna single cell

keyword
https://www.readbyqxmd.com/read/29161231/epigenetic-regulation-mechanisms-of-microrna-expression
#1
Sara Morales, Mariano Monzo, Alfons Navarro
MicroRNAs (miRNAs) are single-stranded RNAs of 18-25 nucleotides that regulate gene expression at the post-transcriptional level. They are involved in many physiological and pathological processes, including cell proliferation, apoptosis, development and carcinogenesis. Because of the central role of miRNAs in the regulation of gene expression, their expression needs to be tightly controlled. Here, we summarize the different mechanisms of epigenetic regulation of miRNAs, with a particular focus on DNA methylation and histone modification...
November 21, 2017: Biomolecular Concepts
https://www.readbyqxmd.com/read/29156756/mir-221-regulated-kit-level-by-wild-type-or-leukemia-mutant-runx1-a-determinant-of-single-myeloblast-fate-decisions-that-collectively-drives-or-hinders-granulopoiesis
#2
Stefano Rossetti, Michael J Anauo, Nicoletta Sacchi
RUNX1, a master transcription factor of hematopoiesis, was shown to orchestrate both cell proliferation and differentiation during granulopoiesis by regulating microRNAs (miRs). In this study, taking advantage of the miR-ON reporter system, we monitored first, how the granulocyte colony stimulation factor (GCSF) temporally modulates the concomitant level variation of miR-221 and one of its prototypic targets, the stem cell factor receptor KIT, in single 32D(miR-ON-221) myeloblasts expressing wild type RUNX1...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29152147/microrna-370-inhibits-the-growth-and-metastasis-of-lung-cancer-by-down-regulating-epidermal-growth-factor-receptor-expression
#3
Xin Liu, You-Guang Huang, Cong-Guo Jin, Yong-Chun Zhou, Xiao-Qun Chen, Jia Li, Yan Chen, Mei Li, Qian Yao, Ke Li, Min Lan, Jia-Gui Ye, Xi-Cai Wang
Abnormal microRNA-370 (miR-370) expression has been frequently reported in several types of cancers, including lung cancer. However, the role and molecular mechanisms of miR-370 in regulating the growth and metastasis of lung cancer have not been clarified. Here, we show higher levels of epidermal growth factor receptor (EGFR), but lower levels of miR-370 expression in most human lung cancer cells and non-tumor cells. Induction of miR-370 over-expression significantly reduced the levels of EGFR expression and the EGFR 3'untranslated region (UTR)-regulated luciferase activity in XWLC-05 and H157 cells, suggesting that miR-370 may bind to the 3'UTR of EGFR mRNA...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29142608/upregulation-of-microrna-18b-induces-phosphatase-and-tensin-homolog-to-accelerate-the-migration-and-invasion-abilities-of-ovarian-cancer
#4
Xiaofang Han, Yan Zhang, Dong Wang, Xinyun Fu, Meiqing Li, Aiming Wang
Ovarian cancer (OC) is the most common cause of mortality from malignant gynecological cancers. Its lethality is mainly a result of tumors that are difficult to detect at the early stage and a lack of effective systemic therapy for advanced status cancer. MicroRNAs (miRNAs/miRs) are a category of single-stranded non-coding small RNAs that bind to their target mRNAs, and aberrant expression levels of miRNAs may serve key roles in regulating cell migration and invasion of various types of human cancer. Previous studies have demonstrated that miR-18b may function as an oncogene in numerous types of tumors, but its role and molecular mechanism in OC remained unclear...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29136645/a-4-mirna-signature-to-predict-survival-in-glioblastomas
#5
Simon K Hermansen, Mia D Sørensen, Anker Hansen, Steen Knudsen, Alvaro G Alvarado, Justin D Lathia, Bjarne W Kristensen
Glioblastomas are among the most lethal cancers; however, recent advances in survival have increased the need for better prognostic markers. microRNAs (miRNAs) hold great prognostic potential being deregulated in glioblastomas and highly stable in stored tissue specimens. Moreover, miRNAs control multiple genes representing an additional level of gene regulation possibly more prognostically powerful than a single gene. The aim of the study was to identify a novel miRNA signature with the ability to separate patients into prognostic subgroups...
2017: PloS One
https://www.readbyqxmd.com/read/29136142/the-forkhead-transcription-factor-foxo3-negatively-regulates-natural-killer-cell-function-and-viral-clearance-in-myocarditis
#6
Madlen Loebel, Luise Holzhauser, Jelka A Hartwig, Praphulla C Shukla, Konstantinos Savvatis, Alexander Jenke, Martina Gast, Felicitas Escher, Sonya C Becker, Sandra Bauer, Andrea Stroux, Antje Beling, Meike Kespohl, Sandra Pinkert, Henry Fechner, Uwe Kuehl, Dirk Lassner, Wolfgang Poller, Heinz-Peter Schultheiss, Tanja Zeller, Stefan Blankenberg, Anna-Pia Papageorgiou, Stephane Heymans, Ulf Landmesser, Carmen Scheibenbogen, Carsten Skurk
Aims: Foxo3 is a transcription factor involved in cell metabolism, survival, and inflammatory disease. However, mechanistic insight in Foxo3 effects is still limited. Here, we investigated the role of Foxo3 on natural killer (NK) cell responses and its effects in viral myocarditis. Methods and results: Effects of Foxo3 on viral load and immune responses were investigated in a model of coxsackie virus B3 myocarditis in wild-type (WT) and Foxo3 deficient mice. Reduced immune cell infiltration, viral titres, and pro-inflammatory cytokines in cardiac tissue were observed in Foxo3-/- mice 7 days post-infection (p...
November 9, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29122960/mir-335-overexpression-impairs-insulin-secretion-through-defective-priming-of-insulin-vesicles
#7
Vishal A Salunkhe, Jones K Ofori, Nikhil R Gandasi, Sofia A Salö, Sofia Hansson, Markus E Andersson, Anna Wendt, Sebastian Barg, Jonathan L S Esguerra, Lena Eliasson
MicroRNAs contribute to the maintenance of optimal cellular functions by fine-tuning protein expression levels. In the pancreatic β-cells, imbalances in the exocytotic machinery components lead to impaired insulin secretion and type 2 diabetes (T2D). We hypothesize that dysregulated miRNA expression exacerbates β-cell dysfunction, and have earlier shown that islets from the diabetic GK-rat model have increased expression of miRNAs, including miR-335-5p (miR-335). Here, we aim to determine the specific role of miR-335 during development of T2D, and the influence of this miRNA on glucose-stimulated insulin secretion and Ca(2+)-dependent exocytosis...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29121557/a-ratiometric-electrochemical-biosensor-for-the-exosomal-micrornas-detection-based-on-bipedal-dna-walkers-propelled-by-locked-nucleic-acid-modified-toehold-mediate-strand-displacement-reaction
#8
Jing Zhang, Liang-Liang Wang, Mei-Feng Hou, Yao-Kun Xia, Wen-Hui He, An Yan, Yun-Ping Weng, Lu-Peng Zeng, Jing-Hua Chen
Sensitive and selective detection of microRNAs (miRNAs) in cancer cells derived exosomes have attracted rapidly growing interest owing to their potential in diagnostic and prognostic applications. Here, we design a ratiometric electrochemical biosensor based on bipedal DNA walkers for the attomolar detection of exosomal miR-21. In the presence of miR-21, DNA walkers are activated to walk continuously along DNA tracks, resulting in conformational changes as well as considerable increases of the signal ratio produced by target-respond and target-independent reporters...
November 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29120349/magnetic-beads-based-sensor-with-tailored-sensitivity-for-rapid-and-single-step-amperometric-determination-of-mirnas
#9
Eva Vargas, Rebeca M Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Eloy Povedano, María Pedrero, Juan J Montoya, Susana Campuzano, José M Pingarrón
This work describes a sensitive amperometric magneto-biosensor for single-step and rapid determination of microRNAs (miRNAs). The developed strategy involves the use of direct hybridization of the target miRNA (miRNA-21) with a specific biotinylated DNA probe immobilized on streptavidin-modified magnetic beads (MBs), and labeling of the resulting heteroduplexes with a specific DNA-RNA antibody and the bacterial protein A (ProtA) conjugated with an horseradish peroxidase (HRP) homopolymer (Poly-HRP40) as an enzymatic label for signal amplification...
November 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29118466/associations-of-functional-microrna-binding-site-polymorphisms-in-il23-th17-inflammatory-pathway-genes-with-gastric-cancer-risk
#10
Kaiyan Dong, Yajuan Xu, Qian Yang, Jiachen Shi, Jicheng Jiang, Yi Chen, Chunhua Song, Kaijuan Wang
IL23/Th17 axis acts as an inflammatory pathway in gastric carcinogenesis. MicroRNA- (miRNA-) binding site single-nucleotide polymorphisms (SNPs) of inflammatory genes may alter gastric cancer (GC) susceptibility. In this study, four miRNA binding site SNPs (rs3748067 of IL17A, rs887796, rs1468488 of IL17RA, and rs10889677 of IL23R) were genotyped from 500 patients and 500 controls. Unconditional logistic regression analyses and multifactor dimensionality reduction software were used to evaluate the relationships of SNPs with GC and gene-environment interactions, respectively...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/29109067/the-insertion-in-the-double-stranded-rna-binding-domain-of-human-drosha-is-important-for-its-function
#11
Xiaoxiao Zhang, Peng Li, Jian Lin, Haochu Huang, Bin Yin, Yan Zeng
microRNAs (miRNAs) are first transcribed as long, primary transcripts, which are then processed by multiple enzymes and proteins to generate the single-stranded, approximately 22-nucleotide (nt)-long mature miRNAs. A critical step in animal miRNA biogenesis is the cleavage of primary miRNA transcripts (pri-miRNAs) to produce precursor miRNAs (pre-miRNAs) by the enzyme Drosha. How Drosha recognizes its substrates remains incompletely understood. In this study we constructed a series of human Drosha mutants and examined their enzymatic activities and interaction with RNAs...
November 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29103762/mirna-genetic-variants-as-potential-diagnostic-biomarkers-for-oral-cancer
#12
Divya Tandon, Jayant Dewangan, Sonal Srivastava, Vivek Kumar Garg, Srikanta Kumar Rath
MicroRNAs (miRNAs) comprise a novel class of small, non-coding endogenous RNAs that have a role in the plethora of regulatory activities by directing their target mRNAs for degradation or translational repression. Single nucleotide polymorphisms (SNPs) in miRNA genes can lead to alteration in mRNA expression, resulting in serious consequences. Detection of miRNA-polymorphisms can potentially improve diagnosis, treatment, prognosis in patients and has extreme implications in the fields of pharmacogenomics and personalization of medicine...
October 10, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29100439/genetic-polymorphisms-in-pre-mirnas-predict-the-survival-of-non-small-cell-lung-cancer-in-chinese-population-a-cohort-study-and-a-meta-analysis
#13
Lingzi Xia, Zhihua Yin, Xuelian Li, Yangwu Ren, Haibo Zhang, Yuxia Zhao, Baosen Zhou
Background: To explore the association of genetic polymorphisms in pre-miRNA 30c-1 rs928508 and pre-miRNA 27a rs895819 with non-small-cell lung cancer prognosis. Materials and Methods: 480 patients from five hospitals were enrolled in this prospective cohort study. They were followed up for five years. The association between genotypes and overall survival was assessed by Cox proportional hazards regression models. A meta-analysis was conducted to provide evidence for the effect of microRNA 27a rs895819 on cancer survival...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100431/impact-of-microrna-34a-and-polymorphism-of-its-target-gene-ca9-on-susceptibility-to-uterine-cervical-cancer
#14
Shun-Fa Yang, Yu-Fan Liu, Chao-Wen Cheng, Wei-En Yang, Wea-Lung Lin, Jiunn-Liang Ko, Po-Hui Wang
The purposes of this study were to associate the genetic polymorphisms in carbonic anhydrase (CA) 9 with uterine cervical cancer and identify the clinical implications. Three single-nucleotide polymorphisms (SNPs), rs2071676 (+201, G/A), rs3829078 (+1081, A/G), and rs1048638 (+1584, C/A), and an 18-base-pair deletion/insertion (376del393) in CA9 were examined. We used the Boyden chamber assay to evaluate the influence of CA9 on the migration of cervical cancers. Tissue microarrays were used to evaluate CAIX immunoreactivity and determine its clinical significance...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29099138/multiplexed-detection-of-micrornas-by-a-competitive-dna-microarray-based-resonance-light-scattering-assay
#15
Xia Liu, Rongrong Tian, Jiaxue Gao, Dianjun Liu, Zhenxin Wang
The reliable detection and monitoring of microRNAs (miRNAs) are of great significance for gaining a better understanding of the functions of miRNAs in a wide range of biological processes. In this work, a competitive DNA microarray-based resonance light scattering (RLS) assay has been developed for the multiplexed detection of miRNAs with relatively high sensitivity and selectivity. After one-step competition hybridization reactions of miRNAs and multiple single strand DNA (ssDNA) conjugated gold nanoparticles (ssDNAsn@GNPs) with immobilized ssDNA probes on the dendrimer-modified slide, the captured ssDNAsn@GNPs are further enlarged through silver deposition...
November 3, 2017: Analyst
https://www.readbyqxmd.com/read/29098857/an-ultrasensitive-electrochemiluminescence-biosensing-platform-for-detection-of-multiple-types-of-biomarkers-toward-identical-cancer-on-a-single-interface
#16
Yamin Nie, Pu Zhang, Haijun Wang, Ying Zhuo, Ya-Qin Chai, Ruo Yuan
Electrochemiluminescence (ECL) with high sensitivity and excellent controllability provides a promising approach for ultrasensitive detection of multiple biomarkers. However, the detection for multiple types of biomarkers on a single interface remains considerable challenge owing to the functional differentiation of different types of biomarkers. Herein, we utilized "on-off-on" switching, target-induced cleavage of peptide, and TdT (terminal deoxynucleoside transferase)-mediated extension successfully constructing a novel ECL biosensor for the ultrasensitive detection of microRNA-141 (miRNA-141) and matrix metalloproteinase-2 (MMP-2)...
November 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29095552/mirna-expression-profile-of-mucoepidermoid-carcinoma
#17
Nada O Binmadi, John R Basile, Paola Perez, Alessia Gallo, Mayank Tandon, Wael Elias, Shyh-Ing Jang, Ilias Alevizos
OBJECTIVES: MicroRNAs (miRNAs) are single-stranded RNAs that have been implicated in cancer initiation and progression and act as tumour suppressors or oncogenes. In this study, miRNA profiling was conducted on the most frequent malignancy of salivary glands, mucoepidermoid carcinoma (MEC), with comparison to normal tissues. MATERIALS AND METHODS: The TaqMan Human miRNA Cards Array was used for the miRNA profiling of MEC and normal tissues. To validate the differentially expressed miRNAs in MEC, we used real-time PCR (qRT-PCR)...
November 2, 2017: Oral Diseases
https://www.readbyqxmd.com/read/29095457/attomolar-detection-of-extracellular-micrornas-released-from-living-prostate-cancer-cells-by-a-plasmonic-nanowire-interstice-sensor
#18
Siyeong Yang, Hongki Kim, Kyung Jin Lee, Seul Gee Hwang, Eun-Kyung Lim, Juyeon Jung, Tae Jae Lee, Hee-Sung Park, Taejoon Kang, Bongsoo Kim
Prostate cancer (PC) is the second leading cause of cancer death for men worldwide. The serum prostate-specific antigen level test has been widely used to screen for PC. This method, however, exhibits a high false-positive rate, leading to over-diagnosis and over-treatment of PC patients. Extracellular microRNAs (miRNAs) recently provided valuable information including the site and the status of the cancers and thus emerged as new biomarkers for several cancers. Among them, miR141 and miR375 are the most pronounced biomarkers for the diagnosis of high-risk PC...
November 16, 2017: Nanoscale
https://www.readbyqxmd.com/read/29078295/intercellular-mrna-trafficking-via-membrane-nanotube-like-extensions-in-mammalian-cells
#19
Gal Haimovich, Christopher M Ecker, Margaret C Dunagin, Elliott Eggan, Arjun Raj, Jeffrey E Gerst, Robert H Singer
RNAs have been shown to undergo transfer between mammalian cells, although the mechanism behind this phenomenon and its overall importance to cell physiology is not well understood. Numerous publications have suggested that RNAs (microRNAs and incomplete mRNAs) undergo transfer via extracellular vesicles (e.g., exosomes). However, in contrast to a diffusion-based transfer mechanism, we find that full-length mRNAs undergo direct cell-cell transfer via cytoplasmic extensions characteristic of membrane nanotubes (mNTs), which connect donor and acceptor cells...
November 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29061957/negative-correlation-between-hepatitis-c-virus-hcv-and-let-7-microrna-family-in-transplanted-livers-the-role-of-rs868-single-nucleotide-polymorphism
#20
Emir Ahmed Sajjad, Marek Radkowski, Agnieszka Perkowska-Ptasińska, Marek Pacholczyk, Magdalena Durlik, Mikołaj Fedorowicz, Renata Pietrzak, Bogna Ziarkiewicz-Wróblewska, Paweł Włodarski, Jacek Malejczyk
BACKGROUND Genetic alterations of TGF-β pathway members, including its transmembrane receptor, TGFBR1, may influence the course of HCV infection. Rs868 is a single-nucleotide polymorphism of the 3'UTR region of TGFBR1, located in a binding site for the conserved let-7/miR98 microRNA family. Previously, we demonstrated a favorable course of hepatitis C recurrence after liver transplantation in rs868 AG genotype of the transplanted liver when compared to rs868 AA. The aim of the present study was to confirm the biological effect of rs868...
October 24, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
keyword
keyword
30886
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"