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https://www.readbyqxmd.com/read/29164070/generation-of-an-attenuated-tiantan-vaccinia-virus-strain-by-deletion-of-multiple-genes
#1
Yiquan Li, Yilong Zhu, Shuang Chen, Wenjie Li, Xunzhe Yin, Shanzhi Li, Pengpeng Xiao, Jicheng Han, Xiao Li, Lili Sun, Ningyi Jin
An attenuated vaccinia virus-MVTTEAB-was constructed by deletion of non-essential gene segments related to the immunomodulatory and virulence functions of the vaccinia virus Tiantan strain (VVTT). The shuttle plasmids pTC-EGFP, pTE-EGFP, pTA35-EGFP, pTB-EGFP, and pTA66-EGFP were constructed and combined with the early and late strong promoter pE/L and EGFP as an exogenous selectable marker. Then, through the homologous recombination technology and Cre/loxP system, the following gene fragments were gradually knocked out one by one: TC7L-TK2L, TE3L, TA35R, TB13R, and TA66R...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29161410/autonomy-affinity-and-the-assessment-of-damages-acb-v-thomson-medical-pte-ltd-2017-sgca-20-and-shaw-v-kovak-2017-ewca-civ-1028
#2
Craig Purshouse
In ACB v Thomson Medical Pte Ltd [2017] SGCA 20 and Shaw v Kovak [2017] EWCA Civ 1028, the idea that 'lost autonomy' should be recognised as a new form of actionable damage in the tort of negligence was rejected in Singapore and England, respectively. This, it will be argued, was the correct outcome. Protecting an interest in autonomy via the tort of negligence would undermine the coherence of that tort. In ACB, however, a new, different, form of damage was recognised: loss of 'genetic affinity'. This commentary will discuss some problems that protecting an interest in 'genetic affinity' raises before critiquing the approach to assessing damages in ACB...
November 17, 2017: Medical Law Review
https://www.readbyqxmd.com/read/29157517/balloon-pulmonary-angioplasty-for-chronic-thromboembolic-pulmonary-hypertension
#3
REVIEW
Ehtisham Mahmud, Omid Behnamfar, Lawrence Ang, Mitul P Patel, David Poch, Nick H Kim
Chronic thromboembolic pulmonary hypertension (CTEPH) is associated with several risk factors but is most frequently seen as a rare consequence of an acute pulmonary embolism. Surgical pulmonary thromboendarterectomy (PTE) is potentially curative for CTEPH with the best outcomes seen for the treatment of primarily proximal, accessible lobar or segmental disease. For surgically inoperable patients, percutaneous balloon pulmonary angioplasty (BPA) is feasible and has good short- to mid-term efficacy outcomes...
January 2018: Interventional cardiology clinics
https://www.readbyqxmd.com/read/29155389/effect-of-the-polyelectrolyte-coating-on-the-photothermal-efficiency-of-gold-nanorods-and-the-photothermal-induced-cancer-cell-damage
#4
Rashmi Shrivastava, Alok Dube
Coating gold nanorods (GNRs) with polyelectrolytes is an effective approach to make them biocompatible for potential use in photothermal treatment (PTT) of cancer. The authors report the effect of coating of the GNRs with polystyrene sulphonate (PSS-GNRs) and PSS plus poly di-allyl di-methyl ammonium chloride (PDDAC-GNRs) on its photothermal conversion efficiency (PTE), cellular uptake and subsequently the photothermal induced cytotoxicity in human oral cancer cells (NT8e). Coating of GNRs with PSS led to decrease in PTE by ∼30% and further coating it with PDDAC led to its increase to similar level, with respect to as- prepared GNRs...
December 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29144431/bioabsorption-and-bioaccumulation-of-cadmium-in-the-straw-and-grain-of-maize-zea-mays-l-in-growing-soils-contaminated-with-cadmium-in-different-environment
#5
Jorge Retamal-Salgado, Juan Hirzel, Ingrid Walter, Iván Matus
There is a worldwide increase of heavy metal or potentially toxic element (PTE), contamination in agricultural soils caused mainly by human and industrial action, which leads to food contamination in crops such as in maize. Cadmium (Cd) is a PTE often found in soils and it is ingested through food. It is necessary to determine the bioabsorption, distribution, and accumulation levels in maize to reduce or prevent food chain contamination. Cadmium absorption and accumulation in three maize cultivars were evaluated in three agricultural environments in Chile by increasing CdCl₂ rates (0, 1, and 2 mg·kg(-1))...
November 16, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29128858/relation-between-lifespan-polytrauma-typologies-and-post-trauma-mental-health
#6
Ateka A Contractor, Lily A Brown, Nicole H Weiss
BACKGROUND: Most individuals experience more than one trauma. Hence, it is important to consider the count and types of traumas (polytraumatization) in relation to post-trauma mental health. METHOD: The current study examined the relation of polytraumatization patterns to PTSD clusters (intrusions, avoidance, negative alterations in cognitions and mood [NACM], and alterations in arousal and reactivity [AAR]), depression, and impulsivity facets (lack of perseverance, lack of premeditation, negative urgency, sensation seeking) using a web-based sample of 346 participants...
October 16, 2017: Comprehensive Psychiatry
https://www.readbyqxmd.com/read/29107365/controlled-burn-and-immediate-mobilization-of-potentially-toxic-elements-in-soil-from-a-legacy-mine-site-in-central-victoria-australia
#7
Joji Abraham, Kim Dowling, Singarayer Florentine
Conducting controlled burns in fire prone areas is an efficient and economic method for forest management, and provides relief from the incidence of high severity wild fires and the consequent damage to human property and ecosystems. However, similar to wild fires, controlled burns also affect many of the physical and biogeochemical properties of the forest soil and may facilitate remobilization of potentially toxic elements (PTEs) sequestered in vegetation and soil organic matter. The objective of the current study is to investigate the mobilization of PTEs, in Central Victorian forest soils in Australia after a controlled burn...
October 26, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29101855/comparative-effects-of-biochar-nanosheets-and-conventional-organic-amendments-on-health-risks-abatement-of-potentially-toxic-elements-via-consumption-of-wheat-grown-on-industrially-contaminated-soil
#8
Balal Yousaf, Guijian Liu, Qumber Abbas, Habib Ullah, Ruwei Wang, Muhammad Zia-Ur-Rehman, Amina, Zhiyuan Niu
Potentially toxic elements (PTEs) discharge to the soil environment through increased anthropogenic activities is a global threat. These PTEs can have harmful and chronic-persistent health effects on exposed populations through food consumption grown on contaminated soils. Efforts to investigate the transformation mechanism and accumulation behavior of PTEs in soil-plant system and their adverse health-effects have focused extensively in previous studies. However, limited studies address biochar nanosheets (BCNs) as a potential soil amendment to reduced humans health risks through dietary intake of food-crop grown on PTE-contaminated soil...
October 26, 2017: Chemosphere
https://www.readbyqxmd.com/read/29069044/diagnostic-value-of-the-corrected-qt-difference-between-leads-v1-and-v6-in-patients-with-acute-pulmonary-thromboembolism
#9
Seong Jun Park, Chang Hee Kwon, Byeong Joo Bae, Bum Sung Kim, Sung Hea Kim, Hyun-Joong Kim, Hweung Kon Hwang, Sang-Man Chung
In acute pulmonary thromboembolism (PTE), right ventricular pressure overload impairs right-sided cardiac conduction and repolarization. We hypothesized that if heterogeneity of repolarization between right and left ventricles occurs in acute PTE, there would be the difference of repolarization between them. Therefore, we aimed to evaluate the diagnostic value of corrected QT interval (QTc) difference between leads V1 and V6 (V1 - V6) in patients with acute PTE.A total of 89 patients with suspected acute PTE who underwent computed tomographic angiography (CTA) were enrolled from January to December 2015...
October 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29058280/bootstrapping-data-envelopment-analysis-of-efficiency-and-productivity-of-county-public-hospitals-in-eastern-central-and-western-china-after-the-public-hospital-reform
#10
Man-Li Wang, Hai-Qing Fang, Hong-Bing Tao, Zhao-Hui Cheng, Xiao-Jun Lin, Miao Cai, Chang Xu, Shuai Jiang
China implemented the public hospital reform in 2012. This study utilized bootstrapping data envelopment analysis (DEA) to evaluate the technical efficiency (TE) and productivity of county public hospitals in Eastern, Central, and Western China after the 2012 public hospital reform. Data from 127 county public hospitals (39, 45, and 43 in Eastern, Central, and Western China, respectively) were collected during 2012-2015. Changes of TE and productivity over time were estimated by bootstrapping DEA and bootstrapping Malmquist...
October 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/29056891/polygala-tenuifolia-extract-inhibits-lipid-accumulation-in-3t3-l1-adipocytes-and-high-fat-diet-induced-obese-mouse-model-and-affects-hepatic-transcriptome-and-gut-microbiota-profiles
#11
Chun-Chung Wang, Jui-Hung Yen, Yi-Cheng Cheng, Chia-Yu Lin, Cheng-Ta Hsieh, Rung-Jiun Gau, Shu-Jiau Chiou, Hwan-You Chang
Obesity, the excessive accumulation of lipids in the body, is closely associated with many prevalent human disorders. Continued efforts to identify plant extracts that exhibit anti-obesity effects have drawn much attention. This study investigated whether a Polygala tenuifolia extract (PTE) possesses anti-obesity activity and how PTE may affect liver gene expression and gut microbiota. We used 3T3-L1 adipocytes and a high-fat diet-induced obese mouse model to determine the effects of PTE on lipid accumulation...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/29049053/treatment-options-for-posttraumatic-epilepsy
#12
Lara L Zimmermann, Ryan M Martin, Fady Girgis
PURPOSE OF REVIEW: Posttraumatic seizures (PTS) and posttraumatic epilepsy (PTE) are common and debilitating consequences of traumatic brain injury (TBI). Early PTS result in secondary brain injury by raising intracranial pressure and worsening cerebral edema and metabolic crisis. PTE is a localization-related epilepsy strongly associated with TBI severity, but risk factors for PTE and epileptogenesis are incompletely understood and are active areas of research. Medical management of PTS in adults and children is reviewed...
December 2017: Current Opinion in Neurology
https://www.readbyqxmd.com/read/29044967/comparison-of-long-term-outcomes-of-50-mg-and-100-mg-rt-pa-in-the-management-of-acute-pulmonary-thromboembolism
#13
Elif Yilmazel Ucar, Omer Araz, Bugra Kerget, Nafiye Yilmaz, Metin Akgun, Leyla Saglam
BACKGROUND: Pulmonary thromboembolism (PTE) is a life-threatening disease. In this study, we aimed to evaluate long-term outcomes of the use of 50 mg recombinant tissue-type plasminogen activator (rt-PA) in the management of PTE in terms of relapse, pulmonary hypertension (PH), mortality and hemorrhage, and to compare with the use of 100 mg rt-PA. METHODS: The study was designed as a retrospective cohort. Patients who were diagnosed as acute PTE and received either 50 mg or 100 mg rt-PA in a tertiary care hospital between 2010 and 2015 were included in the study...
October 17, 2017: Clinical Respiratory Journal
https://www.readbyqxmd.com/read/29026416/right-ventricular-thrombosis-as-a-manifestation-of-beh%C3%A3-et-s-syndrome
#14
Payam Ebrahimifar, Javad Shahabi
BACKGROUND: Behçet's disease (BD) is a rare condition with a classic triad of oral and genital ulceration and eye disease. Cardiovascular complication is a rare finding in BD. CASE REPORT: In this report, we present a seventeen years old patient with a history of fever for 20 days, who developed a clot in right ventricle (RV). Cardiac magnetic resonance imaging (MRI) and echocardiography demonstrated a thrombosis in RV and a thoracic multi detector computed tomographic image showed pulmonary thromboembolism (PTE) in patient...
March 2017: ARYA Atherosclerosis
https://www.readbyqxmd.com/read/28992539/cross-lagged-associations-between-posttraumatic-stress-symptoms-and-coping-self-efficacy-in-long-term-recovery-a-four-wave-comparative-study
#15
Mark W G Bosmans, Peter G van der Velden
RATIONALE: Trauma-related coping self-efficacy (CSE) - in brief, the perceived ability to cope with the aftermath of a traumatic event - has been shown to affect psychological outcomes. A previous study showed that CSE affects subsequent PTSS levels, but not vice versa among a sample exposed 0-6 months ago. OBJECTIVE: In this four-wave follow-up study we examined the cross-lagged relationships between CSE and posttraumatic stress symptoms (PTSS) among those exposed to PTEs (potentially traumatic events) 1-2 years before T1 in order to examine direction of influence...
November 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/28981586/gabapentin-prevents-progressive-increases-in-excitatory-connectivity-and-epileptogenesis-following-neocortical-trauma
#16
D K Takahashi, Sha Jin, D A Prince
Neocortical injury initiates a cascade of events, some of which result in maladaptive epileptogenic reorganization of surviving neural circuits. Research focused on molecular and organizational changes that occur following trauma may reveal processes that underlie human post-traumatic epilepsy (PTE), a common and unfortunate consequence of traumatic brain injury. The latency between injury and development of PTE provides an opportunity for prophylactic intervention, once the key underlying mechanisms are understood...
June 26, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28965282/factors-affecting-the-quality-of-life-before-and-after-surgery-in-patients-with-chronic-thromboembolic-pulmonary-hypertension
#17
Oksana Kamenskaya, Asya Klinkova, Irina Loginova, Alexander Chernyavskiy, Vladimir V Lomivorotov, Alexander Karaskov
PURPOSE: To test the hypothesis that quality of life (QoL) parameters before surgery in patients with chronic thromboembolic pulmonary hypertension (CTEPH) are influenced by clinical determinants related to the underlying disease and to examine QoL parameters affected in the long-term after the operation by complications presenting in the early postoperative period. METHODS: This prospective cohort study included 128 patients who presented with CTEPH before and after pulmonary thromboendarterectomy (PTE; 1-year follow-up)...
September 30, 2017: Quality of Life Research
https://www.readbyqxmd.com/read/28938141/amendment-damages-the-function-of-continuous-flooding-in-decreasing-cd-and-pb-uptake-by-rice-in-acid-paddy-soil
#18
Xinxin Ye, Hongying Li, Ligan Zhang, Rushan Chai, Renfeng Tu, Hongjian Gao
Combinations of remediation technologies are needed to solve the problem of soil contamination in paddy rice, due to multiple potential toxic elements (PTEs). Two potential mitigation methods, water management and in-situ remediation by soil amendment, have been widely used in treatment of PTE-polluted paddy soil. However, the interactive relationship between soil amendment and water management, and its influence on the accumulation of PTEs in rice are poorly understood. Greenhouse pot experiments were conducted to examine the effects of phosphate amendment on Cd and Pb availability in soil and their influence on Cd and Pb uptake into rice, on Fe and P availability in soil, and on the alteration of Fe amount and compartment on root surface among different water management strategies...
January 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28936586/role-of-structured-individual-patient-education-in-the-prevention-of-vascular-complications-in-newly-diagnosed-type-2-diabetes-the-individual-therapeutic-education-in-newly-diagnosed-type-2-diabetes-intend-randomized-controlled-trial
#19
Adriana Coppola, Livio Luzi, Tiziana Montalcini, Andrea Giustina, Carmine Gazzaruso
OBJECTIVE: Recent studies showed that a structured patient therapeutic education (PTE) may decrease both mortality and the development of diabetes complications. Nevertheless, no data are available in the literature on the impact of individual PTE on the complications in newly diagnosed type 2 diabetic patients. Aim of the present randomized controlled trial is to evaluate the impact of individual PTE on the occurrence of macrovascular complications in newly diagnosed type 2 diabetic patients when compared to usual care (UC) and group PTE...
September 21, 2017: Endocrine
https://www.readbyqxmd.com/read/28934492/concerted-action-of-two-3-cap-independent-translation-enhancers-increases-the-competitive-strength-of-translated-viral-genomes
#20
Zhiyou Du, Olga M Alekhina, Konstantin S Vassilenko, Anne E Simon
Several families of plant viruses evolved cap-independent translation enhancers (3'CITE) in the 3' untranslated regions of their genomic (g)RNAs to compete with ongoing cap-dependent translation of cellular mRNAs. Umbravirus Pea enation mosaic virus (PEMV)2 is the only example where three 3'CITEs enhance translation: the eIF4E-binding Panicum mosaic virus-like translational enhancer (PTE) and ribosome-binding 3' T-shaped structure (TSS) have been found in viruses of different genera, while the ribosome-binding kl-TSS that provides a long-distance interaction with the 5' end is unique...
September 19, 2017: Nucleic Acids Research
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