keyword
MENU ▼
Read by QxMD icon Read
search

carbenoxolone

keyword
https://www.readbyqxmd.com/read/29754959/human-induced-pluripotent-stem-cell-derived-cardiac-cell-sheets-expressing-genetically-encoded-voltage-indicator-for-pharmacological-and-arrhythmia-studies
#1
Naim Shaheen, Assad Shiti, Irit Huber, Rami Shinnawi, Gil Arbel, Amira Gepstein, Noga Setter, Idit Goldfracht, Amit Gruber, Snizhanna V Chorna, Lior Gepstein
Fulfilling the potential of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes for studying conduction and arrhythmogenesis requires development of multicellular models and methods for long-term repeated tissue phenotyping. We generated confluent hiPSC-derived cardiac cell sheets (hiPSC-CCSs), expressing the genetically encoded voltage indicator ArcLight. ArcLight-based optical mapping allowed generation of activation and action-potential duration (APD) maps, which were validated by mapping the same hiPSC-CCSs with the voltage-sensitive dye, Di-4-ANBDQBS...
May 7, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29745255/pannexin-1-channels-on-endothelial-cells-mediate-vascular-inflammation-during-lung-ischemia-reperfusion-injury
#2
Ashish K Sharma, Eric J Charles, Yunge Zhao, Adishesh K Narahari, Pranav K Baderdinni, Miranda E Good, Ulrike M Lorenz, Irving L Kron, Douglas A Bayliss, Kodi S Ravichandran, Brant E Isakson, Victor E Laubach
Ischemia-reperfusion injury (IRI), which involves inflammation, vascular permeability, and edema, remains a major challenge after lung transplantation. Pannexin 1 (Panx1) channels modulate cellular ATP release during inflammation. This study tests the hypothesis that endothelial Panx1 is a key mediator of vascular inflammation and edema after IR and that IRI can be blocked by Panx1 antagonism. A murine hilar ligation model of IRI was utilized whereby left lungs underwent 1 hour of ischemia and 2 hours of reperfusion...
May 10, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29729730/dual-functional-extracellular-recording-using-a-light-addressable-potentiometric-sensor-for-bitter-signal-transduction
#3
Liping Du, Jian Wang, Wei Chen, Luhang Zhao, Chunsheng Wu, Ping Wang
This paper presents a dual functional extracellular recording biosensor based on a light-addressable potentiometric sensor (LAPS). The design and fabrication of this biosensor make it possible to record both extracellular membrane potential changes and ATP release from a single taste bud cell for the first time. For detecting ATP release, LAPS chip was functionalized with ATP-sensitive DNA aptamer by covalent immobilization. Taste bud cells isolated from rat were cultured on LAPS surface. When the desired single taste bud cell was illuminated by modulated light, ATP release from single taste bud cells can be measured by recording the shifts of bias voltage-photocurrent curves (I-V curves) when the LAPS chip is working in discrete mode...
August 31, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29676177/acetylcholine-induced-ciliary-beat-of-the-human-nasal-mucosa-is-regulated-by-the-pannexin-1-channel-and-purinergic-p2x-receptor
#4
Ba H Do, Toyoaki Ohbuchi, Tetsuro Wakasugi, Hiroki Koizumi, Mitsuru Yokoyama, Nobusuke Hohchi, Hideaki Suzuki
Background Airway mucociliary transport is an important function for the clearance of inhaled foreign particulates in the respiratory tract. The present study aimed at investigating the regulatory mechanism of acetylcholine (Ach)-induced ciliary beat of the human nasal mucosa in ex vivo. Methods The inferior turbinate mucosa was collected from patients with chronic hypertrophic rhinitis during endoscopic surgery. The mucosa was cut into thin strips, and ciliary movement was observed under a phase-contrast light microscope with a high-speed digital video camera...
January 1, 2018: American Journal of Rhinology & Allergy
https://www.readbyqxmd.com/read/29660499/modulating-membrane-fluidity-corrects-batten-disease-phenotypes-in-vitro-and-in-vivo
#5
Mark L Schultz, Luis Tecedor, Elena Lysenko, Shyam Ramachandran, Colleen S Stein, Beverly L Davidson
The neuronal ceroid lipofuscinoses are a class of inherited neurodegenerative diseases characterized by the accumulation of autofluorescent storage material. The most common neuronal ceroid lipofuscinosis has juvenile onset with rapid onset blindness and progressive degeneration of cognitive processes. The juvenile form is caused by mutations in the CLN3 gene, which encodes the protein CLN3. While mouse models of Cln3 deficiency show mild disease phenotypes, it is apparent from patient tissue- and cell-based studies that its loss impacts many cellular processes...
April 13, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29626652/participation-of-satellite-glial-cells-of-the-dorsal-root-ganglia-in-acute-nociception
#6
Júlia Borges Paes Lemes, Tais de Campos Lima, Débora Oliveira Santos, Amanda Ferreira Neves, Fernando Silva de Oliveira, Carlos Almicar Parada, Celina Monteiro da Cruz Lotufo
At dorsal root ganglia, neurons and satellite glial cells (SGC) can communicate through ATP release and P2X7 receptor activation. SGCs are also interconnected by gap junctions and have been previously implicated in modulating inflammatory and chronic pain.We now present evidence that SGCs are also involved in processing acute nociception in rat dorsal root ganglia. Using primary dorsal root ganglia cultures we observed that calcium transients induced in neurons by capsaicin administration were followed by satellite glial cells activation...
April 4, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29615749/blocking-distinct-interactions-between-glioblastoma-cells-and-their-tissue-microenvironment-a-novel-multi-targeted-therapeutic-approach
#7
Melanie Mettang, Viola Meyer-Pannwitt, Georg Karpel-Massler, Shaoxia Zhou, Neil O Carragher, Karl Josef Föhr, Bernd Baumann, Lisa Nonnenmacher, Stefanie Enzenmüller, Meike Dahlhaus, Markus D Siegelin, Sebastien Stroh, Daniel Mertens, Pamela Fischer-Posovszky, E Marion Schneider, Marc-Eric Halatsch, Klaus-Michael Debatin, Mike-Andrew Westhoff
Due to the highly invasive nature of Glioblastoma (GB), complete surgical resection is not feasible, while motile tumour cells are often associated with several specific brain structures that enhance treatment-resistance. Here, we investigate the therapeutic potential of Disulfiram and Carbenoxolone, that inhibit two distinct interactions between GB and the brain tissue microenvironment: stress-induced cell-matrix adhesion and gap junction mediated cell-cell communication, respectively. Increase in cell numbers of tumour-initiating cells, which are cultured in suspension as cell clusters, and adherent differentiated cells can be blocked to a similar extent by Carbenoxolone, as both cell populations form gap junctions, but the adherent differentiated cells are much more sensitive to Disulfiram treatment, which - via modulation of NF-κB signalling - interferes with cell-substrate adhesion...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29592783/synergistic-activation-of-erk1-2-between-a-fiber-neurons-and-glial-cells-in-the-drg-contributes-to-pain-hypersensitivity-after-tissue-injury
#8
Shunsuke Yamakita, Yasuhiko Horii, Hitomi Takemura, Yutaka Matsuoka, Ayahiro Yamashita, Yosuke Yamaguchi, Megumi Matsuda, Teiji Sawa, Fumimasa Amaya
Background Intense nociceptive signaling arising from ongoing injury activates primary afferent nociceptive systems to generate peripheral sensitization. ERK1/2 phosphorylation in dorsal root ganglion can be used to visualize intracellular signal activity immediately after noxious stimulation. The aim of this study was to investigate spatiotemporal characteristics of ERK1/2 phosphorylation against tissue injury in the primary afferent neurons. Methods Plantar incisions were made in the hind paws of Sprague-Dawley rats (n =150)...
January 2018: Molecular Pain
https://www.readbyqxmd.com/read/29587860/roles-of-astrocytic-connexin-43-hemichannels-and-gap-junctions-in-oxygen-glucose-deprivation-reperfusion-injury-induced-neuroinflammation-and-the-possible-regulatory-mechanisms-of-salvianolic-acid-b-and-carbenoxolone
#9
Xiang Yin, Liangshu Feng, Di Ma, Ping Yin, Xinyu Wang, Shuai Hou, Yulei Hao, Jingdian Zhang, Meiying Xin, Jiachun Feng
BACKGROUND: Glia-mediated neuroinflammation is related to brain injury exacerbation after cerebral ischemia/reperfusion (I/R) injury. Astrocytic hemichannels or gap junctions, which were mainly formed by connexin-43, have been implicated in I/R damage. However, the exact roles of astrocytic hemichannels and gap junction in neuroinflammatory responses induced by I/R injury remain unknown. METHODS: Primary cultured astrocytes were subjected to OGD/R injury, an in vitro model of I/R injury...
March 27, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29587265/transforming-growth-factor-beta-1-drives-a-switch-in-connexin-mediated-cell-to-cell-communication-in-tubular-cells-of-the-diabetic-kidney
#10
Claire Hills, Gareth William Price, Mark John Wall, Timothy John Kaufmann, Sidney Chi-Wai Tang, Wai Han Yiu, Paul Edward Squires
BACKGROUND/AIMS: Changes in cell-to-cell communication have been linked to several secondary complications of diabetes, but the mechanism by which connexins affect disease progression in the kidney is poorly understood. This study examines a role for glucose-evoked changes in the beta1 isoform of transforming growth factor (TGFβ1), on connexin expression, gap-junction mediated intercellular communication (GJIC) and hemi-channel ATP release from tubular epithelial cells of the proximal renal nephron...
March 26, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29559915/circadian-atp-release-in-organotypic-cultures-of-the-rat-suprachiasmatic-nucleus-is-dependent-on-p2x7-and-p2y-receptors
#11
Irena Svobodova, Anirban Bhattaracharya, Milorad Ivetic, Zdenka Bendova, Hana Zemkova
The circadian rhythms in physiological and behavioral functions are driven by a pacemaker located in the suprachiasmatic nucleus (SCN). The rhythms continue in constant darkness and depend on cell-cell communication between neurons and glia. The SCN astrocytes generate also a circadian rhythm in extracellular adenosine 5'-triphosphate (ATP) accumulation, but molecular mechanisms that regulate ATP release are poorly understood. Here, we tested the hypothesis that ATP is released via the plasma membrane purinergic P2X7 receptors (P2X7Rs) and P2Y receptors (P2YRs) which have been previously shown to be expressed in the SCN tissue at transcriptional level...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29534516/tat-gap19-and-carbenoxolone-alleviate-liver-fibrosis-in-mice
#12
Sara Crespo Yanguas, Tereza C da Silva, Isabel V A Pereira, Joost Willebrords, Michaël Maes, Marina Sayuri Nogueira, Inar Alves de Castro, Isabelle Leclercq, Guilherme R Romualdo, Luís F Barbisan, Luc Leybaert, Bruno Cogliati, Mathieu Vinken
Although a plethora of signaling pathways are known to drive the activation of hepatic stellate cells in liver fibrosis, the involvement of connexin-based communication in this process remains elusive. Connexin43 expression is enhanced in activated hepatic stellate cells and constitutes the molecular building stone of hemichannels and gap junctions. While gap junctions support intercellular communication, and hence the maintenance of liver homeostasis, hemichannels provide a circuit for extracellular communication and are typically opened by pathological stimuli, such as oxidative stress and inflammation...
March 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29521811/carbenoxolone-as-a-novel-therapy-for-attenuation-of-cancer-induced-bone-pain
#13
Sarah Falk
Pain is a major complication for patients with cancer significantly compromising their quality of life. Current treatment is far from optimal and particularly bone-related cancer pain poses an increasing clinical and socioeconomical problem. Connexins, key proteins in cell-cell communication, have the potential to affect cancer-induced bone pain at multiple levels, including nociceptive signaling and bone degradation. This study tested the analgesic potential of carbenoxolone, a broad-acting connexin blocker, in a mouse model of cancer-induced bone pain...
June 2018: Pain
https://www.readbyqxmd.com/read/29484398/pannexin-1-is-involved-in-neuronal-apoptosis-and-degeneration-in-experimental-intracerebral-hemorrhage-in-rats
#14
Linqiang Zhou, Chenglin Liu, Zhong Wang, Haitao Shen, Zunjia Wen, Dongdong Chen, Qing Sun, Gang Chen
Pannexins serve an important role in the regulation of extracellular neuronal regenerative currents and cellular signal transduction of glial cells; however, the effects of pannexins in various cerebrovascular diseases have not been reported. The present study focused on the expression and influence of pannexins in a rat model of intracerebral hemorrhage (ICH), and confirmed that pannexins (including Pannexin‑1, Pannexin‑2 and Pannexin‑3) are expressed in rat brain tissues. However, only the expression of Pannexin‑1 was significantly increased and peaked 48 h post‑ICH...
April 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29458137/bisphenol-a-alters-oocyte-maturation-by-prematurely-closing-gap-junctions-in-the-cumulus-cell-oocyte-complex
#15
Deyanira Guadalupe Acuña-Hernández, Laura Arreola-Mendoza, Ramsés Santacruz-Márquez, Sihomara Patricia García-Zepeda, Lyda Yuliana Parra-Forero, Jesús Alberto Olivares-Reyes, Isabel Hernández-Ochoa
In ovarian follicles, cumulus cells communicate with the oocyte through gap junction intercellular communication (GJIC), to nurture the oocyte and control its meiosis arrest and division. Bisphenol A (BPA) is a monomer found in polycarbonate-made containers that can induce functional alterations, including impaired oocyte meiotic division and reduced molecule transfer in GJIC. However, how BPA alters oocyte meiotic division is unclear. We investigated whether BPA effects on oocyte meiotic division were correlated with reduced transfer in GJIC...
April 1, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29456683/carbenoxolone-inhibits-mechanical-stress-induced-osteogenic-differentiation-of-mesenchymal-stem-cells-by-regulating-p38-mapk-phosphorylation
#16
Shenglong Li, Jing Wang, Yudi Han, Xiaoteng Li, Changjian Liu, Zhengshuai Lv, Xiuhui Wang, Xin Tang, Zhe Wang
The aim of the present study was to explore the effects of pannexin1 (Px1) protein channels on osteogenic differentiation of mesenchymal stem cells (MSCs) under mechanical stress stimulation. MSCs were isolated from Sprague Dawley rats (3 weeks old, weighing 100-120 g) and cultured in vitro . A safe concentration of carbenoxolone was determined (CBX, an inhibitor of Px1 channels; 100 µM) on MSCs using the Cell Counting Kit-8 (CCK8) method. MSCs were divided into 6 groups: Control, stress (4,000 µ strain), and stress following 3, 6, 12, and 24 h pretreatment with CBX...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29418022/acute-anti-obesity-effects-of-intracerebroventricular-11%C3%AE-hsd1-inhibitor-administration-in-diet-induced-obese-mice
#17
M Seo, S A Islam, S-S Moon
The hypothalamus is the regulatory centre of both appetite and energy balance and endoplasmic reticulum (ER) stress in the hypothalamus is involved in the pathogenesis of obesity. Recently, inhibition of 11 β hydroxysteroid dehydrogenase type1 (11β-HSD1) was reported to have an anti-obesity effect by reducing fat mass. However, the link between the role of 11β-HSD1 in the hypothalamus and obesity has yet to be determined. In the present study, embryonal primary hypothalamic neurones and high-fat diet (HFD) fed mice were used to investigate the anorexigenic effects of 11β-HSD1 inhibitors both in vitro and in vivo...
March 2018: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/29393654/trpv4-stimulation-releases-atp-via-pannexin-channels-in-human-pulmonary-fibroblasts
#18
Mozibur Rahman, Rui Sun, Subhendu Mukherjee, Bernd Nilius, Luke J Janssen
We previously described several ionic conductances in human pulmonary fibroblasts (HPFs), including one activated by two structurally distinct TRPV4-channel agonists: 4α-phorbol-12,13-didecanoate (4αPDD) and GSK1016790A. However, the TRPV4-activated current exhibited peculiar properties: it developed slowly over many minutes, exhibited reversal potentials that could vary by tens of millivolts even within a given cell, and was not easily reversed by subsequent addition of two distinct TRPV4-selective blockers (RN-1734 or HC-067047)...
February 2, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29366904/expression-and-function-of-connexin-43-protein-in-mouse-and-human-retinal-pigment-epithelial-cells-as-hemichannels-and-gap-junction-proteins
#19
Shin-Ichi Akanuma, Hideyuki Higashi, Souhei Maruyama, Koji Murakami, Masanori Tachikawa, Yoshiyuki Kubo, Ken-Ichi Hosoya
The changes in the transport function of the outer blood-retinal barrier (BRB), formed by retinal pigment epithelial (RPE) cells, under pathological conditions need to be understood to normalize the retinal homeostasis in retinal diseases. Connexin 43 (Cx43) is known to be one of the basic units of gap junctions and hemichannels, which are opened by changes in extracellular conditions. The purpose of this study was to clarify the expression of Cx43 in RPE cells of the retina and Cx43 contribution to compound transport functions in RPE cells...
March 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29298877/molecular-basis-for-potentiation-of-cx36-gap-junction-channel-conductance-by-n-alcohols-and-general-anesthetics
#20
Vytautas Raškevičius, Vaidas Jotautis, Lina Rimkutė, Alina Marandykina, Mintautė Kazokaitė, Visvaldas Kairys, Vytenis Arvydas Skeberdis
In our recent study, we have demonstrated that short carbon chain n -alcohols (up to octanol) stimulated while long carbon chain n -alcohols inhibited the conductance of connexin (Cx) 36 (Cx36) gap junction (GJ) channels. In contrast, GJ channels composed of other types of Cxs all were inhibited by n -alcohols independent of their carbon chain length. To identify the putative structural domains of Cx36, responsible for the dual effect of n -alcohols, we performed structural modeling of Cx36 protein docking with hexanol and isoflurane that stimulated as well as nonanol and carbenoxolone that inhibited the conductance of Cx36 GJs and revealed their multiple common docking sites and a single pocket accessible only to hexanol and isoflurane...
February 28, 2018: Bioscience Reports
keyword
keyword
30865
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"