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https://www.readbyqxmd.com/read/28092639/lithographic-patterning-of-antibodies-by-direct-lift-off-and-improved-surface-adhesion
#1
Sankalp Verma, Vivek Verma
The inherent property of antibodies binding to their antigen with high specificity makes them a strong candidate for sensing and detection applications. Microscale patterning of antibodies is desired for the miniaturization of sensors and fundamental cell biology studies. However, existing methodologies to pattern antibodies at the microscale are multi-step. In this work, we demonstrate microscale patterning of antibodies on a glass coverslip in a single step photolithography process. The microscale features of the photoresist were generated on the coverslip using photolithography, and the antibody solution was incubated...
January 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28091936/task-specific-motor-rehabilitation-therapy-after-stroke-improves-performance-in-a-different-motor-task-translational-evidence
#2
M El Amki, P Baumgartner, O Bracko, A R Luft, S Wegener
While the stroke survivor with a motor deficit strives for recovery of all aspects of daily life movements, neurorehabilitation training is often task specific and does not generalize to movements other than the ones trained. In rodent models of post-stroke recovery, this problem is poorly investigated as the training task is often the same as the one that measures motor function. The present study investigated whether motor training by pellet reaching translates into enhancement of different motor functions in rats after stroke...
January 14, 2017: Translational Stroke Research
https://www.readbyqxmd.com/read/28086977/people-living-with-hiv-on-art-have-accurate-perception-of-lipodystrophy-signs-a-cross-sectional-study
#3
Paulo R Alencastro, Nemora T Barcellos, Fernando H Wolff, Maria Letícia R Ikeda, Fabiana Schuelter-Trevisol, Ajácio B M Brandão, Sandra C Fuchs
BACKGROUND: The prevalence of lipodystrophy ranges from 31 to 65%, depending on the criteria adopted for diagnosis. The usual methods applied in the diagnosis vary from self-perception, medical examination, skinfolds measurements, or even imaging assessment for confirmation of fat distribution changes. Although several methods have been developed, there is no gold standard for characterization of LA and LH, or mixed forms. This study aimed to compare self-reported signs of lipodystrophy with objective measures by skinfolds and circumferences, and to evaluate the prevalence of lipoatrophy (LA) and lipohypertrophy (LH) among subjects living with HIV/AIDS on ART...
January 13, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28079363/fluorescence-development-of-latent-fingerprint-with-conjugated-polymer-nanoparticles-in-aqueous-colloidal-solution
#4
Hong Chen, Rong-Liang Ma, Yun Chen, Li-Juan Fan
Poly(p-phenylene vinylene) (PPV) nanoparticles in aqueous colloidal solution have been prepared via a modified Wessling method, with the addition of surfactant. The fluorescent colloidal solution was used as the developing solution to develop the fingerprints on different substrates. The developing process was accomplished simply by immersing the substrates into developing solution and then taking out, followed by rinsing with deionized water. The initial study about the fingerprints on the adhesive tapes showed that the developing solution is very effective in fluorescence developing on both fresh and aged visible fingerprints; and such effect was negligibly affected by treating the fingerprints with water or other organic solvents, whether before developing or after...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28076206/processing-older-persons-as-clients-in-elderly-care-a-study-of-the-micro-processes-of-care-management-practice
#5
Anna Olaison
Elder care has undergone a marketization in recent years in which various models for care management have been introduced with the aim of making assessments efficient. This article investigates the effects the care management model has on resource allocation for home care when handling the requests of older persons in the needs assessment process. Sixteen tape-recorded assessment conversations with associated case-file texts were analyzed through discourse analysis. The results show that a managerialist thinking has had a partial impact on the assessment process where the documentation requirements have entailed bureaucratization in terms of the transfer that occurs from talk to text...
January 11, 2017: Social Work in Health Care
https://www.readbyqxmd.com/read/28074570/development-of-the-sbirt-checklist-for-observation-in-real-time-score
#6
Janice A Vendetti, Bonnie G McRee, Frances K Del Boca
BACKGROUND AND AIMS: Screening, Brief Intervention and Referral to Treatment (SBIRT) programs have been implemented widely in medical settings, with little attention focused on how well providers adhere to evidence-based service delivery in everyday practice. The purposes of this paper were to: (1) introduce a flexible, relatively simple methodology, the SBIRT Checklist for Observation in Real-time (SCORe), to assess adherence to evidence-based practice and provide preliminary evidence supporting its criterion validity; and (2) illustrate the feasibility and potential utility of the SCORe by analyzing observations of providers within four large-scale SBIRT programs in the United States...
February 2017: Addiction
https://www.readbyqxmd.com/read/28073620/electrochemical-monitoring-of-native-catalase-activity-in-skin-using-skin-covered-oxygen-electrode
#7
Sarah Nocchi, Sebastian Björklund, Birgitta Svensson, Johan Engblom, Tautgirdas Ruzgas
A skin covered oxygen electrode, SCOE, was constructed with the aim to study the enzyme catalase, which is part of the biological antioxidative system present in skin. The electrode was exposed to different concentrations of H2O2 and the amperometric current response was recorded. The observed current is due to H2O2 penetration through the outermost skin barrier (referred to as the stratum corneum, SC) and subsequent catalytic generation of O2 by catalase present in the underlying viable epidermis and dermis...
January 3, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28073351/exploring-neurodevelopmental-outcome-measures-used-in-children-with-cerebral-malaria-the-perspectives-of-caregivers-and-health-workers-in-malawi
#8
Emmie W Mbale, Terrie Taylor, Bernard Brabin, Macpherson Mallewa, Melissa Gladstone
BACKGROUND: Progress has been made in tackling malaria however there are still over 207 million cases worldwide, the majority in children. As survival rates improve, numbers of children with long-term neurodisabling sequelae are likely to increase. Most outcome studies in cerebral malaria (CM) have focused only on body function and structure and less on outcomes within the broader framework of the International Classification of Functioning and Disability (ICF). The aim of this study was to utilise qualitative methods to identify relevant clinical outcomes in CM to support formulation of a core outcome set relevant to CM and other acquired brain injuries for use in future clinical trials...
January 10, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28072901/achilles-tendon-repair-using-the-internalbrace%C3%A2-principle
#9
Graeme P Hopper, Gordon M Mackay
Despite extensive research, the management of acute ruptures of the Achilles tendon remains an area of controversy, and there is no consensus regarding the optimal treatment. An InternalBrace™ (Arthrex, Inc., Naples, Florida) is a ligament augmentation repair using high strength sutures/tape and knotless bone anchors which encourages healing and allows early mobilization. This article describes, with video illustration, a knotless Achilles tendon repair technique using the InternalBrace™ principle.
January 10, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/28072735/localized-type-volkmann-s-contracture-treated-with-tendon-transfer-and-tension-reduced-early-mobilization-a-case-report
#10
Yoshio Kaji, Osamu Nakamura, Konosuke Yamaguchi, Sachiko Tobiume, Tetsuji Yamamoto
RATIONALE: For localized type Volkmann's contracture, in which degeneration of the flexor digitorum profundus (FDP) muscle to one or two fingers and restriction of finger extension occur, dissection or excision of the affected muscle is usually recommended. However, these surgical procedures need relatively wide exposure of the muscle, because the FDP muscle is in the deep portion of the forearm. PATIENT CONCERNS: In this report, the case of a 35-year-old woman with localized type Volkmann's contracture is presented...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28071868/development-and-validation-of-two-equations-based-on-anthropometry-estimating-body-fat-for-the-greek-adult-population
#11
Spyridon Kanellakis, Efstathios Skoufas, Vladlena Khudokonenko, Eftychia Apostolidou, Loukia Gerakiti, Maria-Chrysi Andrioti, Evangelia Bountouvi, Yannis Manios
OBJECTIVE: To validate anthropometric equations in the current literature predicting body fat percentage (%BF) in the Greek population, to develop and validate two anthropometric equations estimating %BF, and to compare them with the retrieved equations. METHODS: Anthropometric data from 642 Greek adults were incorporated. Dual-energy X-ray absorptiometry was used as reference method. The comparison with other equations was made using Bland-Altman analysis, intraclass correlation coefficient, and Lin's concordance correlation coefficient...
January 10, 2017: Obesity
https://www.readbyqxmd.com/read/28070641/influence-of-temporary-nasal-occlusion-tno-on-epistaxis-frequency-in-patients-with-hereditary-hemorrhagic-telangiectasia-hht
#12
Kornelia E C Wirsching, Frank Haubner, Thomas S Kühnel
The objective of the study was to evaluate the influence of temporary nasal occlusion (tNO) with hypoallergenic tape on the frequency and severity of epistaxis in patients with hereditary hemorrhagic telangiectasia (HHT). This prospective observational study included 20 HHT patients who were undergoing Nd:YAG laser therapy at regular intervals. Over a 3-month period, laser therapy was supplemented by tNO with hypoallergenic tape for 5 h/day on average. On a 0-10 numeric rating scale, the patients reported significantly greater satisfaction in epistaxis terms after tNO treatment, with mean scores of 5 before and 7 after 3-month tNO (p = 0...
January 9, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28070457/the-concurrent-validity-and-reliability-of-the-leg-motion-system-for-measuring-ankle-dorsiflexion-range-of-motion-in-older-adults
#13
Carlos Romero Morales, César Calvo Lobo, David Rodríguez Sanz, Irene Sanz Corbalán, Beatriz Ruiz Ruiz, Daniel López López
BACKGROUND: New reliable devices for range of motion (ROM) measures in older adults are necessary to improve knowledge about the functional capability in this population. Dorsiflexion ROM limitation is associated with ankle injuries, foot pain, lower limb disorders, loss of balance, gait control disorders and fall risk in older adults. The aim of the present study was to assess the validity and reliability of the Leg Motion device for measuring ankle dorsiflexion ROM in older adults. METHODS: Adescriptive repeated-measures study was designed to test the reliability of Leg Motion in thirty-three healthy elderly patients older than 65 years...
2017: PeerJ
https://www.readbyqxmd.com/read/28069155/communicating-with-patients-from-minority-backgrounds-individual-challenges-experienced-by-oncology-health-professionals
#14
Kaaren J Watts, Bettina Meiser, Elvira Zilliacus, Rajneesh Kaur, Mona Taouk, Afaf Girgis, Phyllis Butow, David Goldstein, Sandra Hale, Astrid Perry, Sanchia K Aranda, David W Kissane
PURPOSE: Oncology health professionals (HPs) are increasingly required to care for patients from minority backgrounds. Yet many HPs have not had formal training in how to communicate effectively in culturally diverse settings. More information is needed about the challenges that oncology HPs face in communicating with minority patients to inform the content of formal training programs. This qualitative study aimed to identify oncology nurses' and oncologists' individual experiences and challenges in communicating with patients from minority backgrounds...
February 2017: European Journal of Oncology Nursing: the Official Journal of European Oncology Nursing Society
https://www.readbyqxmd.com/read/28068108/transferability-and-adhesion-of-sol-gel-derived-crystalline-tio2-thin-films-to-different-types-of-plastic-substrates
#15
Natsumi Amano, Mitsuru Takahashi, Hiroaki Uchiyama, Hiromitsu Kozuka
Anatase thin films were prepared on various plastic substrates by our recently developed sol-gel transfer technique. The plastic substrates employed were polycarbonate (PC), poly(methyl methacrylate) (PMMA), polyethylene terephthalate (PET), polyethylene (PE), polypropylene (PP), polyether ether ketone (PEEK), and polyvinylidene chloride (PVDC). An Si(100) substrate was first coated with a polyimide (PI)/polyvinylpyrrolidone (PVP) mixture layer, and an alkoxide-derived titania gel film was deposited on it by spin-coating...
January 9, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28067487/investigation-into-pseudo-capacitance-behavior-of-glycoside-containing-hydrogels
#16
Nachiket R Raravikar, Andrew Dobos, Eshwaran Narayanan, Taraka Sai Pavan Grandhi, Saurabh Mishra, Kaushal Rege, Michael Goryll
Electrochemical pseudo-capacitors are an attractive choice for energy storage applications because they offer higher energy densities than electrostatic or electric double layer capacitors. They also offer higher power densities in shorter durations of time, as compared to batteries. Recent efforts on pseudo-capacitors include biocompatible hydrogel electrolytes and transition metal electrodes for implantable energy storage applications. Pseudo-capacitive behavior in these devices has been attributed to the redox reactions that occur within the electric double layer, which is formed at the electrode-electrolyte interface...
January 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28063035/monitoring-skin-dendritic-cells-in-steady-state-and-inflammation-by-immunofluorescence-microscopy-and-flow-cytometry
#17
Julia L Ober-Blöbaum, Daniela Ortner, Bernhard Haid, Anna Brand, Christoph Tripp, Björn E Clausen, Patrizia Stoitzner
Skin dendritic cells (DC) are strategically positioned at the body's second largest epithelial border to the environment. Hence they are the first antigen presenting cells that encounter invading pathogens and environmental antigens, including contact sensitizers and carcinogens penetrating the skin. Moreover, DC have the unique ability to induce immunity or tolerance and thus take center stage in regulating innate and adaptive immune responses. Skin DC can be divided into several phenotypically and functionally distinct subtypes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28062928/evaluation-of-paraxial-forward-scattering-from-intraocular-lens-with-increased-surface-light-scattering-using-goniophotometry-and-hartmann-shack-wavefront-aberrometry
#18
Keiichiro Minami, Yoko Maruyama, Toshifumi Mihashi, Kazunori Miyata, Tetsuro Oshika
PURPOSE: To evaluate the influence of increases in light scattering on intraocular lens (IOL) surfaces on paraxial forward scattering using goniophotometry and Hartmann-Shack wavefront aberrometry. METHODS: Surface light scattering was reproduced experimentally by acceleratedly aging 4 intraocular lenses by 0, 3, 5, and 10 years each. Light scattering from both IOL surfaces was measured using Scheimpflug photography. The paraxial forward scattering from the aged IOLs was measured using a goniophotometer with a halogen light source (wavelength: 350-850 nm) and telecentric optics, and changes in the maximum intensity and full width at 10% of maximum intensity (FW10%) were evaluated...
January 6, 2017: Japanese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28062470/effectiveness-of-an-improvement-programme-to-prevent-interruptions-during-medication-administration-in-a-paediatric-hospital-a-preintervention-postintervention-study
#19
Immacolata Dall'Oglio, Martina Fiori, Vincenzo Di Ciommo, Emanuela Tiozzo, Rachele Mascolo, Natalia Bianchi, Marta Luisa Ciofi Degli Atti, Antonella Ferracci, Orsola Gawronski, Manuel Pomponi, Massimiliano Raponi
OBJECTIVE: To assess the effectiveness of an improvement programme to reduce the number of interruptions during the medication administration process in a paediatric hospital. DESIGN AND METHODS: A prestudy-post study design was used to monitor nursing interruptions during medication cycles in a paediatric hospital. Interruptions were reported on an observation sheet (MADOS-P) adapted to the paediatric context. SETTING: A 600-bed tertiary paediatric research hospital in Italy...
January 6, 2017: BMJ Open
https://www.readbyqxmd.com/read/28061818/perspectives-preferences-and-needs-regarding-early-prediction-of-preeclampsia-in-dutch-pregnant-women-a-qualitative-study
#20
Neeltje M T H Crombag, Marije Lamain-de Ruiter, Anneke Kwee, Peter C J I Schielen, Jozien M Bensing, Gerard H A Visser, Arie Franx, Maria P H Koster
BACKGROUND: To improve early risk-identification in pregnancy, research on prediction models for common pregnancy complications is ongoing. Therefore, it was the aim of this study to explore pregnant women's perceptions, preferences and needs regarding prediction models for first trimester screening for common pregnancy complications, such as preeclampsia, to support future implementation. METHOD: Ten focus groups (of which five with primiparous and five with multiparous women) were conducted (n = 45)...
January 7, 2017: BMC Pregnancy and Childbirth
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