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https://www.readbyqxmd.com/read/29784938/characterization-of-the-rat-cerebrospinal-fluid-proteome-following-acute-cerebral-ischemia-using-an-aptamer-based-proteomic-technology
#1
Alba Simats, Teresa García-Berrocoso, Laura Ramiro, Dolors Giralt, Natalia Gill, Anna Penalba, Alejandro Bustamante, Anna Rosell, Joan Montaner
The limited accessibility to the brain has turned the cerebrospinal fluid (CSF) into a valuable source that may contribute to the complete understanding of the stroke pathophysiology. Here we have described the CSF proteome in the hyper-acute phase of cerebral ischemia by performing an aptamer-based proteomic assay (SOMAscan) in CSF samples collected before and 30 min after male Wistar rats had undergone a 90 min Middle Cerebral Artery Occlusion (MCAO) or sham-surgery. Proteomic results indicated that cerebral ischemia acutely increased the CSF levels of 716 proteins, mostly overrepresented in leukocyte chemotaxis and neuronal death processes...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784083/a-homozygous-loss-of-function-camk2a-mutation-causes-growth-delay-frequent-seizures-and-severe-intellectual-disability
#2
Poh Hui Chia, Franklin Lei Zhong, Shinsuke Niwa, Carine Bonnard, Kagistia Hana Utami, Ruizhu Zeng, Hane Lee, Ascia Eskin, Stanley F Nelson, William H Xie, Samah Al-Tawalbeh, Mohammad El-Khateeb, Mohammad Shboul, Mahmoud A Pouladi, Mohammed Al-Raqad, Bruno Reversade
Calcium/calmodulin-dependent protein kinase II (CAMK2) plays fundamental roles in synaptic plasticity that underlies learning and memory. Here, we describe a new recessive neurodevelopmental syndrome with global developmental delay, seizures and intellectual disability. Using linkage analysis and exome sequencing, we found that this disease maps to chromosome 5q31.1-q34 and is caused by a biallelic germline mutation in CAMK2A . The missense mutation, p.His477Tyr is located in the CAMK2A association domain that is critical for its function and localization...
May 22, 2018: ELife
https://www.readbyqxmd.com/read/29773102/-over-expression-of-bdnf-inhibits-angiotensin-ii-induced-apoptosis-of-cardiomyocytes-in-sd-rats
#3
Jingli Cao, Yingfeng Wu, Geming Liu, Zhenlong Li
Objective To investigate the role and molecular mechanism of brain-derived neurotrophic factor (BDNF) against the process of cardiomyocyte hypertrophy and apoptosis. Methods Cardiomyocyte hypertrophy were estabolished by angiotensin II (Ang II) in neonatal cardiomyocytes in vitro and incomplete ligature of abdominal aorta of SD rats in vivo. BDNF over-expressing recombinant vector pcDNA5-BDNF was transfected into cardiomyocytes by liposomes. Immunofluorescence staining was used to detect the effect of BDNF transfection on the surface area of myocardial cells...
March 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29250091/exogenous-calcium-enhances-the-photosystem-ii-photochemistry-response-in-salt-stressed-tall-fescue
#4
Guangyang Wang, Aoyue Bi, Erick Amombo, Huiying Li, Liang Zhang, Cheng Cheng, Tao Hu, Jinmin Fu
Calcium enhances turfgrass response to salt stress. However, little is known about PSII photochemical changes when exogenous calcium was applied in salinity-stressed turfgrass. Here, we probe into the rearrangements of PSII electron transport and endogenous ion accumulation in tall fescue ( Festuca arundinacea Schreber) treated with exogenous calcium under salt stress. Three-month-old seedlings of genotype "TF133" were subjected to the control (CK), salinity (S), salinity + calcium nitrate (SC), and salinity + ethylene glycol tetraacetic acid (SE)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29233185/phosphoproteomics-reveals-network-rewiring-to-a-pro-adhesion-state-in-annexin-1-deficient-mammary-epithelial-cells
#5
Asfa Alli-Shaik, Sheena Wee, Lina H K Lim, Jayantha Gunaratne
BACKGROUND: Annexin-1 (ANXA1) plays pivotal roles in regulating various physiological processes including inflammation, proliferation and apoptosis, and deregulation of ANXA1 functions has been associated with tumorigenesis and metastasis events in several types of cancer. Though ANXA1 levels correlate with breast cancer disease status and outcome, its distinct functional involvement in breast cancer initiation and progression remains unclear. We hypothesized that ANXA1-responsive kinase signaling alteration and associated phosphorylation signaling underlie early events in breast cancer initiation events and hence profiled ANXA1-dependent phosphorylation changes in mammary gland epithelial cells...
December 12, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29100089/de-novo-mutations-in-protein-kinase-genes-camk2a-and-camk2b-cause-intellectual-disability
#6
Sébastien Küry, Geeske M van Woerden, Thomas Besnard, Martina Proietti Onori, Xénia Latypova, Meghan C Towne, Megan T Cho, Trine E Prescott, Melissa A Ploeg, Stephan Sanders, Holly A F Stessman, Aurora Pujol, Ben Distel, Laurie A Robak, Jonathan A Bernstein, Anne-Sophie Denommé-Pichon, Gaëtan Lesca, Elizabeth A Sellars, Jonathan Berg, Wilfrid Carré, Øyvind Løvold Busk, Bregje W M van Bon, Jeff L Waugh, Matthew Deardorff, George E Hoganson, Katherine B Bosanko, Diana S Johnson, Tabib Dabir, Øystein Lunde Holla, Ajoy Sarkar, Kristian Tveten, Julitta de Bellescize, Geir J Braathen, Paulien A Terhal, Dorothy K Grange, Arie van Haeringen, Christina Lam, Ghayda Mirzaa, Jennifer Burton, Elizabeth J Bhoj, Jessica Douglas, Avni B Santani, Addie I Nesbitt, Katherine L Helbig, Marisa V Andrews, Amber Begtrup, Sha Tang, Koen L I van Gassen, Jane Juusola, Kimberly Foss, Gregory M Enns, Ute Moog, Katrin Hinderhofer, Nagarajan Paramasivam, Sharyn Lincoln, Brandon H Kusako, Pierre Lindenbaum, Eric Charpentier, Catherine B Nowak, Elouan Cherot, Thomas Simonet, Claudia A L Ruivenkamp, Sihoun Hahn, Catherine A Brownstein, Fan Xia, Sébastien Schmitt, Wallid Deb, Dominique Bonneau, Mathilde Nizon, Delphine Quinquis, Jamel Chelly, Gabrielle Rudolf, Damien Sanlaville, Philippe Parent, Brigitte Gilbert-Dussardier, Annick Toutain, Vernon R Sutton, Jenny Thies, Lisenka E L M Peart-Vissers, Pierre Boisseau, Marie Vincent, Andreas M Grabrucker, Christèle Dubourg, Wen-Hann Tan, Nienke E Verbeek, Martin Granzow, Gijs W E Santen, Jay Shendure, Bertrand Isidor, Laurent Pasquier, Richard Redon, Yaping Yang, Matthew W State, Tjitske Kleefstra, Benjamin Cogné, Slavé Petrovski, Kyle Retterer, Evan E Eichler, Jill A Rosenfeld, Pankaj B Agrawal, Stéphane Bézieau, Sylvie Odent, Ype Elgersma, Sandra Mercier
Calcium/calmodulin-dependent protein kinase II (CAMK2) is one of the first proteins shown to be essential for normal learning and synaptic plasticity in mice, but its requirement for human brain development has not yet been established. Through a multi-center collaborative study based on a whole-exome sequencing approach, we identified 19 exceedingly rare de novo CAMK2A or CAMK2B variants in 24 unrelated individuals with intellectual disability. Variants were assessed for their effect on CAMK2 function and on neuronal migration...
November 2, 2017: American Journal of Human Genetics
https://www.readbyqxmd.com/read/29054677/expression-of-the-ppm1f-gene-is-regulated-by-stress-and-associated-with-anxiety-and-depression
#7
Aliza P Wingo, Eric R Velasco, Antonio Florido, Adriana Lori, Dennis C Choi, Tanja Jovanovic, Kerry J Ressler, Raül Andero
BACKGROUND: Molecular mechanisms underlying psychological sequelae of exposure to stressful experiences, such as posttraumatic stress disorder (PTSD) and depression, are not well understood. METHODS: Using convergent evidence from animal and human transcriptomic and genomic studies, we aimed to identify genetic mechanisms underlying depression and anxiety after traumatic experiences. RESULTS: From a transcriptome-wide analysis in mice, we found the Ppm1f gene to be differentially expressed in the amygdala and medial prefrontal cortex (mPFC) a week after immobilization stress...
February 1, 2018: Biological Psychiatry
https://www.readbyqxmd.com/read/28615319/retinoschisin-is-linked-to-retinal-na-k-atpase-signaling-and-localization
#8
Karolina Plössl, Melanie Royer, Sarah Bernklau, Neslihan N Tavraz, Thomas Friedrich, Jens Wild, Bernhard H F Weber, Ulrike Friedrich
Mutations in the RS1 gene cause X-linked juvenile retinoschisis (XLRS), a hereditary retinal dystrophy. We recently showed that retinoschisin, the protein encoded by RS1 , regulates ERK signaling and apoptosis in retinal cells. In this study, we explored an influence of retinoschisin on the functionality of the Na/K-ATPase, its interaction partner at retinal plasma membranes. We show that retinoschisin binding requires the β2-subunit of the Na/K-ATPase, whereas the α-subunit is exchangeable. Our investigations revealed no effect of retinoschisin on Na/K-ATPase-mediated ATP hydrolysis and ion transport...
August 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28181390/tris-1-3-dichloro-2-propyl-phosphate-induces-toxicity-by-stimulating-camk2-in-pc12-cells
#9
Chaonan Li, Li Li, Bencheng Lin, Yanjun Fang, Honglian Yang, Huanliang Liu, Zhuge Xi
Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is one of the widely used organophosphorus flame retardants (OPFRs), which are regarded as suitable substitutes for brominated flame retardants (BFRs). Previously, we have validated the toxicity of TDCIPP in PC12 cells owing to the induced alterations in GAP43, NF-H, CaMK2a/2b, and tubulin α/β proteins; however, limited information is currently available on the toxicity and mechanism of TDCIPP. In the present study, cytotoxicity effects were evaluated by exposing PC12 cells to different concentrations of TDCIPP (0-50 μM) for 4 days...
June 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/27605043/phosphorylation-of-calcium-calmodulin-stimulated-protein-kinase-ii-at-t286-enhances-invasion-and-migration-of-human-breast-cancer-cells
#10
Mengna Chi, Hamish Evans, Jackson Gilchrist, Jack Mayhew, Alexander Hoffman, Elizabeth Ann Pearsall, Helen Jankowski, Joshua Stephen Brzozowski, Kathryn Anne Skelding
Calcium/calmodulin-stimulated protein kinase II (CaMKII) is a multi-functional kinase that controls a range of cellular functions, including proliferation, differentiation and apoptosis. The biological properties of CaMKII are regulated by multi-site phosphorylation. However, the role that CaMKII phosphorylation plays in cancer cell metastasis has not been examined. We demonstrate herein that CaMKII expression and phosphorylation at T286 is increased in breast cancer when compared to normal breast tissue, and that increased CAMK2 mRNA is associated with poor breast cancer patient prognosis (worse overall and distant metastasis free survival)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27278803/post-exercise-cold-water-immersion-does-not-alter-high-intensity-interval-training-induced-exercise-performance-and-hsp72-responses-but-enhances-mitochondrial-markers
#11
Paula Fernandes Aguiar, Sílvia Mourão Magalhães, Ivana Alice Teixeira Fonseca, Vanessa Batista da Costa Santos, Mariana Aguiar de Matos, Marco Fabrício Dias Peixoto, Fábio Yuzo Nakamura, Craig Crandall, Hygor Nunes Araújo, Leonardo Reis Silveira, Etel Rocha-Vieira, Flávio de Castro Magalhães, Fabiano Trigueiro Amorim
This study aims to evaluate the effect of regular post-exercise cold water immersion (CWI) on intramuscular markers of cellular stress response and signaling molecules related to mitochondria biogenesis and exercise performance after 4 weeks of high intensity interval training (HIIT). Seventeen healthy subjects were allocated into two groups: control (CON, n = 9) or CWI (n = 8). Each HIIT session consisted of 8-12 cycling exercise stimuli (90-110 % of peak power) for 60 s followed by 75 s of active recovery three times per week, for 4 weeks (12 HIIT sessions)...
2016: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/27245989/mechanistic-study-of-trpm2-ca-2-camk2-becn1-signaling-in-oxidative-stress-induced-autophagy-inhibition
#12
Qian Wang, Wenjing Guo, Baixia Hao, Xianli Shi, Yingying Lu, Connie W M Wong, Victor W S Ma, Timothy T C Yip, Joseph S K Au, Quan Hao, King-Ho Cheung, Wutian Wu, Gui-Rong Li, Jianbo Yue
Reactive oxygen species (ROS) have been commonly accepted as inducers of autophagy, and autophagy in turn is activated to relieve oxidative stress. Yet, whether and how oxidative stress, generated in various human pathologies, regulates autophagy remains unknown. Here, we mechanistically studied the role of TRPM2 (transient receptor potential cation channel subfamily M member 2)-mediated Ca(2+) influx in oxidative stress-mediated autophagy regulation. On the one hand, we demonstrated that oxidative stress triggered TRPM2-dependent Ca(2+) influx to inhibit the induction of early autophagy, which renders cells more susceptible to death...
August 2, 2016: Autophagy
https://www.readbyqxmd.com/read/27058532/genome-wide-mapping-of-growth-related-quantitative-trait-loci-in-orange-spotted-grouper-epinephelus-coioides-using-double-digest-restriction-site-associated-dna-sequencing-ddradseq
#13
Hui Yu, Xinxin You, Jia Li, Hankui Liu, Zining Meng, Ling Xiao, Haifa Zhang, Hao-Ran Lin, Yong Zhang, Qiong Shi
Mapping of quantitative trait loci (QTL) is essential for the discovery of genetic structures that related to complex quantitative traits. In this study, we identified 264,072 raw SNPs (single-nucleotide polymorphisms) by double digest restriction site associated DNA sequencing (ddRADseq), and utilized 3029 of these SNPs to construct a genetic linkage map in orange-spotted grouper (Epinephelus coioides) using a regression mapping algorithm. The genetic map contained 24 linkage groups (LGs) spanning a total genetic distance of 1231...
April 6, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26829591/a-new-view-of-transcriptome-complexity-and-regulation-through-the-lens-of-local-splicing-variations
#14
Jorge Vaquero-Garcia, Alejandro Barrera, Matthew R Gazzara, Juan González-Vallinas, Nicholas F Lahens, John B Hogenesch, Kristen W Lynch, Yoseph Barash
Alternative splicing (AS) can critically affect gene function and disease, yet mapping splicing variations remains a challenge. Here, we propose a new approach to define and quantify mRNA splicing in units of local splicing variations (LSVs). LSVs capture previously defined types of alternative splicing as well as more complex transcript variations. Building the first genome wide map of LSVs from twelve mouse tissues, we find complex LSVs constitute over 30% of tissue dependent transcript variations and affect specific protein families...
February 1, 2016: ELife
https://www.readbyqxmd.com/read/26160508/exploring-g-protein-coupled-receptor-signaling-networks-using-silac-based-phosphoproteomics
#15
Grace R Williams, Jennifer R Bethard, Mary N Berkaw, Alexis K Nagel, Louis M Luttrell, Lauren E Ball
The type 1 parathyroid hormone receptor (PTH1R) is a key regulator of calcium homeostasis and bone turnover. Here, we employed SILAC-based quantitative mass spectrometry and bioinformatic pathways analysis to examine global changes in protein phosphorylation following short-term stimulation of endogenously expressed PTH1R in osteoblastic cells in vitro. Following 5min exposure to the conventional agonist, PTH(1-34), we detected significant changes in the phosphorylation of 224 distinct proteins. Kinase substrate motif enrichment demonstrated that consensus motifs for PKA and CAMK2 were the most heavily upregulated within the phosphoproteome, while consensus motifs for mitogen-activated protein kinases were strongly downregulated...
January 1, 2016: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/25995477/opposing-role-for-egr3-in-nucleus-accumbens-cell-subtypes-in-cocaine-action
#16
Ramesh Chandra, T Chase Francis, Prasad Konkalmatt, Ariunzaya Amgalan, Amy M Gancarz, David M Dietz, Mary Kay Lobo
An imbalance in molecular signaling cascades and transcriptional regulation in nucleus accumbens (NAc) medium spiny neuron (MSN) subtypes, those enriched in dopamine D1 versus D2 receptors, is implicated in the behavioral responses to psychostimulants. To provide further insight into the molecular mechanisms occurring in MSN subtypes by cocaine, we examined the transcription factor early growth response 3 (Egr3). We evaluated Egr3 because it is a target of critical cocaine-mediated signaling pathways and because Egr3-binding sites are found on promoters of key cocaine-associated molecules...
May 20, 2015: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/25865065/the-acetyltransferase-hat1-moderates-the-nf-%C3%AE%C2%BAb-response-by-regulating-the-transcription-factor-plzf
#17
Anthony J Sadler, Bandar A Suliman, Liang Yu, Xiangliang Yuan, Die Wang, Aaron T Irving, Soroush T Sarvestani, Ashish Banerjee, Ashley S Mansell, Jun-Ping Liu, Steve Gerondakis, Bryan R G Williams, Dakang Xu
To date, the activities of protein kinases have formed the core of our understanding of cell signal transduction. Comprehension of the extent of protein acetylation has raised expectations that this alternate post-transcriptional modification will be shown to rival phosphorylation in its importance in mediating cellular responses. However, limited instances have been identified. Here we show that signalling from Toll-like or TNF-α receptors triggers the calcium/calmodulin-dependent protein kinase (CaMK2) to activate histone acetyltransferase-1 (HAT1), which then acetylates the transcriptional regulator PLZF...
2015: Nature Communications
https://www.readbyqxmd.com/read/25834039/the-knockdown-of-%C3%AE-kap-alters-the-postsynaptic-apparatus-of-neuromuscular-junctions-in-living-mice
#18
Isabel Martinez-Pena Y Valenzuela, Mohamed Aittaleb, Po-Ju Chen, Mohammed Akaaboune
A muscle-specific nonkinase anchoring protein (αkap), encoded within the calcium/calmodulin kinase II (camk2) α gene, was recently found to control the stability of acetylcholine receptor (AChR) clusters on the surface of cultured myotubes. However, it remains unknown whether this protein has any effect on receptor stability and the maintenance of the structural integrity of neuromuscular synapses in vivo. By knocking down the endogenous expression of αkap in mouse sternomastoid muscles with shRNA, we found that the postsynaptic receptor density was dramatically reduced, the turnover rate of receptors at synaptic sites was significantly increased, and the insertion rates of both newly synthesized and recycled receptors into the postsynaptic membrane were depressed...
April 1, 2015: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/25733872/transcription-factor-hes1-modulates-osteoarthritis-development-in-cooperation-with-calcium-calmodulin-dependent-protein-kinase-2
#19
Shurei Sugita, Yoko Hosaka, Keita Okada, Daisuke Mori, Fumiko Yano, Hiroshi Kobayashi, Yuki Taniguchi, Yoshifumi Mori, Tomotake Okuma, Song Ho Chang, Manabu Kawata, Shuji Taketomi, Hirotaka Chikuda, Haruhiko Akiyama, Ryoichiro Kageyama, Ung-Il Chung, Sakae Tanaka, Hiroshi Kawaguchi, Shinsuke Ohba, Taku Saito
Notch signaling modulates skeletal formation and pathogenesis of osteoarthritis (OA) through induction of catabolic factors. Here we examined roles of Hes1, a transcription factor and important target of Notch signaling, in these processes. SRY-box containing gene 9 (Sox9)-Cre mice were mated with Hes1(fl/fl) mice to generate tissue-specific deletion of Hes1 from chondroprogenitor cells; this deletion caused no obvious abnormality in the perinatal period. Notably, OA development was suppressed when Hes1 was deleted from articular cartilage after skeletal growth in type II collagen (Col2a1)-Cre(ERT);Hes1(fl/fl) mice...
March 10, 2015: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/25730670/mitochondrial-control-by-drp1-in-brain-tumor-initiating-cells
#20
Qi Xie, Qiulian Wu, Craig M Horbinski, William A Flavahan, Kailin Yang, Wenchao Zhou, Stephen M Dombrowski, Zhi Huang, Xiaoguang Fang, Yu Shi, Ashley N Ferguson, David F Kashatus, Shideng Bao, Jeremy N Rich
Brain tumor initiating cells (BTICs) co-opt the neuronal high affinity glucose transporter, GLUT3, to withstand metabolic stress. We investigated another mechanism critical to brain metabolism, mitochondrial morphology, in BTICs. BTIC mitochondria were fragmented relative to non-BTIC tumor cell mitochondria, suggesting that BTICs increase mitochondrial fission. The essential mediator of mitochondrial fission, dynamin-related protein 1 (DRP1), showed activating phosphorylation in BTICs and inhibitory phosphorylation in non-BTIC tumor cells...
April 2015: Nature Neuroscience
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