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https://www.readbyqxmd.com/read/27924413/suppression-of-cell-wall-degrading-enzymes-and-their-encoding-genes-in-button-mushrooms-agaricus-bisporus-by-cacl2-and-citric-acid
#1
Zia Ullah Khan, Li Jiayin, Nasir Mehmood Khan, Wangshu Mou, Dongdong Li, Yansheng Wang, Simin Feng, Zisheng Luo, Linchun Mao, Tiejin Ying
Fresh button mushrooms (Agaricus bisporus) were harvested and treated with a solution of 1.5% CaCl2 + 0.5% citric acid and stored for 16 days at 12 °C. The effects of this treatment on firmness, weight, color, cell wall compositions (cellulose and chitin) and cell wall degrading enzymes (cel1ulase, beta-1, 3 glucanase, chitinase and phenylalanine ammonialyase) were investigated during post-harvest storage. The expressions of major genes (Cel1, Glu1, Chi1 and PAL1) involved in cell wall degradation during post-harvest storage were also monitored...
December 6, 2016: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/27922131/different-responses-of-alpine-plants-to-nitrogen-addition-effects-on-plant-plant-interactions
#2
Jun Wang, Peng Luo, Hao Yang, Chengxiang Mou, Li Mo
The different responses of plant species to resource stress are keys to understand the dynamics of plant community in a changing environment. To test the hypothesis that nitrogen (N) increase would benefit N competitive species, rather than N stress-tolerant species, to compete with neighbours, we conducted an experiment with neighbour removal, N addition and soil moisture as treatments in an alpine grassland on the southeastern Tibetan Plateau. Both growths and competitive-response abilities (CRA, the ability to tolerate the inhibitory effects of neighbors) of Kobresia macrantha, Polygonum viviparum and Potentilla anserine in wet site were facilitated by N addition, conversely, both growths and CRA of Taraxacum mongolicum and Ligularia virgaurea were suppressed by N addition, indicating that the responses of CRA of target species under N addition were consistent with the N utilization strategies of them...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27922000/membrane-pyrophosphatases-from-thermotoga-maritima-and-vigna-radiata-suggest-a-conserved-coupling-mechanism
#3
Kun-Mou Li, Craig Wilkinson, Juho Kellosalo, Jia-Yin Tsai, Tommi Kajander, Lars J C Jeuken, Yuh-Ju Sun, Adrian Goldman
Membrane-bound pyrophosphatases (M-PPases), which couple proton/sodium ion transport to pyrophosphate synthesis/hydrolysis, are important in abiotic stress resistance and in the infectivity of protozoan parasites. Here, three M-PPase structures in different catalytic states show that closure of the substrate-binding pocket by helices 5-6 affects helix 13 in the dimer interface and causes helix 12 to move down. This springs a 'molecular mousetrap', repositioning a conserved aspartate and activating the nucleophilic water...
December 6, 2016: Nature Communications
https://www.readbyqxmd.com/read/27920126/postnatal-activation-of-tlr4-in-astrocytes-promotes-excitatory-synaptogenesis-in-hippocampal-neurons
#4
Yi Shen, Huaping Qin, Juan Chen, Lingyan Mou, Yang He, Yixiu Yan, Hang Zhou, Ya Lv, Zhong Chen, Junlu Wang, Yu-Dong Zhou
Astrocytes are critical in synapse development, and their dysfunction in crucial developmental stages leads to serious neurodevelopmental diseases, including seizures and epilepsy. Immune challenges not only affect brain development, but also promote seizure generation and epileptogenesis, implying immune activation is one of the key factors linking seizures and epilepsy to abnormal brain development. In this study, we report that activating astrocytes by systemic lipopolysaccharide (LPS) challenges in the second postnatal week promotes excitatory synapse development, leading to enhanced seizure susceptibility in mice...
December 5, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27917657/two-new-phenylpropanoids-from-swertia-atroviolacea-and-their-anti-5%C3%AE-reductase-activity
#5
Hai-Yan Wu, Xue-Qing Kan, Yu Sheng, Lin-Yun Mou, Qing-Song Yang, Qiu-Feng Hu, Gan-Peng Li
Two new phenylpropanoids (1-2), together with two known lignans (3-4), were isolated from whole herb of Swertia atroviolacea. The structures of the new metabolites were established on the basis of detailed spectroscopic analysis. Compounds 1 and 2 were evaluated for their anti-5α-reductase activity. The results revealed that 1 and 2 showed weak activity with reductase inhibitions of 45.6 ± 2.8% and 38.4 ± 2.5%, respectively.
December 5, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27916229/corrigendum-to-long-term-leptin-treatment-exerts-a-pro-apoptotic-effect-on-renal-tubular-cells-via-prostaglandin-e2-augmentation-eur-j-pharmacol-689-2012-65-71
#6
Yung-Ho Hsu, Chung-Yi Cheng, Yen-Cheng Chen, Tso-Hsiao Chen, Yuh-Mou Sue, Wei-Lun Tsai, Cheng-Hsien Chen
No abstract text is available yet for this article.
December 5, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27904014/male-infertility-related-molecules-involved-in-sperm-oocyte-fusion
#7
Lisha Mou, Ni Xie
Male infertility has become a very serious problem in the human reproduction system, but the molecular mechanism of infertility remains largely unknown. Fertilization is the phenomenon in which a sperm and oocyte find each other, interact, and fuse. Sperm-oocyte fusion-related factors on the sperm side play crucial roles in male infertility. For example, IZUMO1 is well-known as a sperm protein essential for fusion of a sperm and oocyte, but its dysfunction or mutation can result in male infertility. Recent studies showed a novel sperm protein named Bactericidal/permeability-increasing protein (BPI), which takes part in the sperm-oocyte fusion process...
December 1, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27901487/hoxb7-overexpression-promotes-cell-proliferation-and-correlates-with-poor-prognosis-in-gastric-cancer-patients-by-inducing-expression-of-both-akt-and-marks
#8
Xujun He, Zhengchuang Liu, Yingjie Xia, Ji Xu, Guocai Lv, Lu Wang, Tonghui Ma, Liping Jiang, Yiping Mou, Xiaoting Jiang, Jie Ma, Zhongkuo Zhao, Haibin Ni, Wenjuan Xu, Guoqing Ru, Dongsheng Huang, Houquan Tao
Increased expression of HOXB7 has been reported to correlate with the progression in many cancers. However, the specific mechanism by which it promotes the evolution of gastric cancer (GC) is poorly understood.In this study, we sought to investigate the role of HOXB7 in GC by assessing HOXB7 expression in patient tissue and its correlation to clinical characteristics. We found that GC tissues showed increased expression of HOXB7 and that the HOXB7 expression was significantly associated with Lauren classification, invasion depth, lymphatic metastasis and poor prognosis, and could serve as an independent prognostic factor...
November 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/27899331/comparison-of-7t-and-3t-mri-in-patients-with-moyamoya-disease
#9
Byeong Ho Oh, Hyeong Cheol Moon, Hyeon Man Baek, Youn Joo Lee, Sang Woo Kim, Young Jai Jeon, Gun Seok Lee, Hong Rae Kim, Jai Ho Choi, Kyung Soo Min, Mou Seop Lee, Young Gyu Kim, Dong Ho Kim, Won Seop Kim, Young Seok Park
Magnetic resonance imaging and magnetic resonance angiography (MRI/MRA) are widely used for evaluating the moyamoya disease (MMD). This study compared the diagnostic accuracy of 7Tesla (T) and 3T MRI/MRA in MMD. In this case control study, 12 patients [median age: 34years; range (10-66years)] with MMD and 12 healthy controls [median age: 25years; range (22-59years)] underwent both 7T and 3T MRI/MRA. To evaluate the accuracy of MRI/MRA in MMD, five criteria were compared between imaging systems of 7T and 3T: Suzuki grading system, internal carotid artery (ICA) diameter, ivy sign, flow void of the basal ganglia on T2-weighted images, and high signal intensity areas of the basal ganglia on time-of-flight (TOF) source images...
November 27, 2016: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/27898345/quercetin-loading-cdse-zns-nanoparticles-as-efficient-antibacterial-and-anticancer-materials
#10
Xiaofang Yang, Weiwei Zhang, Zhiwei Zhao, Nuan Li, Zhipeng Mou, Dongdong Sun, Yongping Cai, Weiyun Wang, Yi Lin
Quercetin (Qe) plays an important role in inflammation, antibacterial, anticancer, and aging. However, Qe has extremely low water solubility, which is a major challenge in drug absorption. In this study, we described a simple method for synthesis of Qe/CdSe/ZnS nanoparticles (QCZ NPs). The QCZ NPs had an average diameter of 10nm and prominent yellow emission under UV irradiation. We investigated the antibacterial activity of QCZ NPs against drug-resistant Escherichia coli (E. coli) and Bacillus subtilis (B...
November 17, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27896150/early-reoperations-after-primary-repair-of-jejunoileal-atresia-in-newborns
#11
Fanny Yeung, Yuk Him Tam, Yuen Shan Wong, Siu Yan Tsui, Hei Yi Wong, Kristine Kit Yi Pang, Christopher H Houben, Jennifer Wai Cheung Mou, Kin Wai Chan, Kim Hung Lee
Aim: To review nine-year experience in managing jejuno-ileal atresia (JIA) by primary resection and anastomosis and identify factors associated with reoperations. Methods: From April 2006 to May 2015, all consecutive neonates who underwent bowel resection and primary anastomosis for JIA were analyzed retrospectively. Patients with temporary enterostomy were excluded. Patient demographics, types of atresia, surgical techniques, need for reoperations, and long-term outcomes were investigated. Results: A total of forty-three neonates were included, in which nineteen (44...
October 2016: Journal of Neonatal Surgery
https://www.readbyqxmd.com/read/27895790/corosolic-acid-inhibits-the-proliferation-of-osteosarcoma-cells-by-inducing-apoptosis
#12
Yong Jia, Hua Yuan, Shouqin Shan, Gang Xu, Jie Yu, Chenguang Zhao, Xiang Mou
Corosolic acid (CRA), a pentacyclic triterpene isolated from medicinal herbs, has been reported to exhibit anticancer properties in several cancers. However, the anticancer activity of CRA in osteosarcoma cells is still unclear. In the present study, the inhibitory effect of CRA in osteosarcoma MG-63 cells was investigated, and the results revealed that CRA significantly inhibited the viability of MG-63 cells in a dose- and time-dependent manner. A typical apoptotic hallmark such as DNA ladder was detected by agarose gel electrophoresis following treatment with CRA...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895402/effect-of-airplane-transport-of-donor-livers-on-post-liver-transplantation-survival
#13
Yi Huang, Gerry MacQuillan, Leon A Adams, George Garas, Megan Collins, Albert Nwaba, Linjun Mou, Max K Bulsara, Luc Delriviere, Gary P Jeffrey
AIM: To evaluate the effect of long haul airplane transport of donor livers on post-transplant outcomes. METHODS: A retrospective cohort study of patients who received a liver transplantation was performed in Perth, Australia from 1992 to 2012. Donor and recipient characteristics information were extracted from Western Australian liver transplantation service database. Patients were followed up for a mean of six years. Patient and graft survival were evaluated and compared between patients who received a local donor liver and those who received an airplane transported donor liver...
November 7, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27889952/effects-of-different-doses-of-2-3-dimercaptosuccinic-acid-modified-fe2-o3-nanoparticles-on-intercalated-discs-in-engineered-cardiac-tissues
#14
Yongchao Mou, Shuanghong Lv, Fei Xiong, Yao Han, Yuwei Zhao, Junjie Li, Ning Gu, Jin Zhou
Although iron oxide nanoparticles (IRONs) were applied in clinical magnetic resonance imaging in vivo and magnetic tissue engineering in vitro widely, the underlying effects of IRONs on the development of cardiomyocytes especially the intercellular junctions, intercalated discs (IDs), remain an unknown issue. Given the critical role of three-dimensional (3D) engineered cardiac tissues (ECTs) in evaluation of nanoparticles toxicology, it remained necessary to understand the effects of IRONs on IDs assembly of cardiomyocytes in 3D environment...
November 27, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27885609/methods-for-genome-wide-methylome-profiling-of-campylobacter-jejuni
#15
Kathy T Mou, Tyson A Clark, Usha K Muppirala, Andrew J Severin, Paul J Plummer
Methylation has a profound role in the regulation of numerous biological processes in bacteria including virulence. The study of methylation in bacteria has greatly advanced thanks to next-generation sequencing technologies. These technologies have expedited the process of uncovering unique features of many bacterial methylomes such as characterizing previously uncharacterized methyltransferases, cataloging genome-wide DNA methylations in bacteria, identifying the frequency of methylation at particular genomic loci, and revealing regulatory roles of methylation in the biology of various bacterial species...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27881207/perceived-stress-and-social-adaptation-of-the-primary-caregivers-of-children-with-intellectual-disabilities
#16
Mohd Ashik Shahrier, Md Nurul Islam, Mou-Mita Debroy
The study aimed to explore whether McCubbin and Patterson's Double ABCX model of stress and adaptation can promote a better understanding of the impacts of social support, definition of the stressor and coping strategies on stress and adaptation of the primary caregivers of Intellectually Disabled (ID) children above and beyond the effects of other factors. Structured Interview Schedule, Perceived Stress Scale (Keya, 2006) and Social Adjustment Scale (Mahmuda & Parvin, 2005) were administered on 130 caregivers of ID children and 140 caregivers of typically developing children recruited conveniently...
November 24, 2016: Spanish Journal of Psychology
https://www.readbyqxmd.com/read/27868335/the-elongator-complex-associated-protein-drl1-plays-a-positive-role-in-immune-responses-against-necrotrophic-fungal-pathogens-in-arabidopsis
#17
Chenggang Wang, Xudong Zhang, Jian-Liang Li, Yanping Zhang, Zhonglin Mou
DEFORMED ROOT AND LEAVES1 (DRL1) is an Arabidopsis homologue of the yeast TOXIN TARGET4 (TOT4)/KILLER TOXIN-INSENSITIVE12 (KTI12) protein that is physically associated with the RNA polymerase II interacting protein complex named Elongator. Mutations in DRL1 and Elongator lead to similar morphological and molecular phenotypes, suggesting that DRL1 and Elongator may functionally overlap in Arabidopsis. We have previously shown that Elongator plays an important role in both salicylic acid (SA)- and jasmonic acid (JA)/ethylene (ET)-mediated defense responses...
November 21, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27867120/transforaminal-resection-of-cervical-dumbbell-schwannomas-in-patients-with-additional-tumors
#18
Ji Zhang, Xiang-Heng Zhang, Zi-Feng Wang, You-Ping Li, Zheng-Quan Zhu, Ji-Cheng Sun, Zheng-He Chen, Shao-Yong Wu, Ke Sai, Jian Wang, Yong-Gao Mou, Zhong-Ping Chen
BACKGROUND: It is rare for two primary tumors to occur simultaneously in a patient. Management of cervical dumbbell schwannomas (CDSs) with concurrent tumors (CTs) requires a specific neurosurgical strategy. The primary objective of this study is to investigate surgical strategies for CDSs with CTs while preserving as much of the mechanically relevant bone structures as possible. MATERIAL AND METHODS: Twelve patients with concurrent CTs and CDSs were identified from three medical centers...
November 17, 2016: World Neurosurgery
https://www.readbyqxmd.com/read/27865455/analysis-of-ustiloxins-in-rice-using-polymer-cation-exchange-cleanup-followed-by-liquid-chromatography-tandem-mass-spectrometry
#19
Zhao-Yun Cao, Li-Hua Sun, Ren-Xiang Mou, Xiao-Yan Lin, Rong Zhou, You-Ning Ma, Ming-Xue Chen
Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Ustilaginoidea virens of rice false smut. Quantification of ustiloxins is essential to assess the food safety of rice infected by rice false smut disease. This paper describes a sensitive method for the simultaneous quantification of ustiloxins A, B, C, D and F in rice grains using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Since notable matrix enhancement effects (21%-78%) occurred for all of the target analytes (except for ustiloxin A), several solid phase extraction materials were tested for their ability to retain ustiloxins from aqueous solutions prior to the LC-MS/MS analysis, including C18 sorbents, polymer anion exchange sorbents resin (PAX), and polymer cation exchange resin (PCX)...
December 9, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27857926/intracerebral-hemorrhagic-infarction-after-cranioplasty-in-a-patient-with-sinking-skin-flap-syndrome
#20
Jai-Hyuck Han, Mou-Seop Lee
The sinking skin flap syndrome (SSFS) is a rare complication after a large craniectomy. Hemorrhage infarction after a cranioplasty is a very rare complication with only 4 cases to date. We report a case of the patient who underwent an autologous cranioplasty to treat SSFS that developed intracerebral hemorrhage infarction. A 20-year-old male was admitted to our emergency department with stuporous mentality. Emergent decompressive craniectomy (DC) have done. He had suffered from SSFS and fever of unknown origin (FUO) since DC...
October 2016: Korean Journal of Neurotrauma
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