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Tony Hunter

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https://www.readbyqxmd.com/read/29118429/mesenchymal-stem-cells-internalize-mycobacterium-tuberculosis-through-scavenger-receptors-and-restrict-bacterial-growth-through-autophagy
#1
Arshad Khan, Lovepreet Mann, Ramesha Papanna, Mi-Ae Lyu, Christopher R Singh, Scott Olson, N Tony Eissa, Jeffrey Cirillo, Gobardhan Das, Robert L Hunter, Chinnaswamy Jagannath
Human mesenchymal stem cells (MSCs) express scavenger receptors that internalize lipids, including oxidized low-density lipoprotein (oxLDL). We report that MSCs phagocytose Mycobacterium tuberculosis (Mtb) through two types of scavenger receptors (SRs; MARCO and SR-B1), as blockade of the receptors with antibodies or siRNA knockdown decreased the uptake of Mtb. MSCs also expressed mannose receptor (MR) that was found to endocytose rhodamine-labeled mannosylated BSA (rMBSA), though the receptor was not involved in the uptake of Mtb...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29104525/acoustic-separation-of-nanoparticles-in-continuous-flow
#2
Mengxi Wu, Zhangming Mao, Kejie Chen, Hunter Bachman, Yuchao Chen, Joseph Rufo, Liqiang Ren, Peng Li, Lin Wang, Tony Jun Huang
The separation of nanoscale particles based on their differences in size is an essential technique to the nanoscience and nanotechnology community. Here, nanoparticles are successfully separated in a continuous flow by using tilted-angle standing surface acoustic waves. The acoustic field deflects nanoparticles based on volume, and the fractionation of nanoparticles is optimized by tuning the cutoff parameters. The continuous separation of nanoparticlesis demonstrated with a ≈90% recovery rate. The acoustic nanoparticle separation method is versatile, non-invasive, and simple...
April 11, 2017: Advanced Functional Materials
https://www.readbyqxmd.com/read/29058706/histidine-kinases-and-the-missing-phosphoproteome-from-prokaryotes-to-eukaryotes
#3
Kevin Adam, Tony Hunter
Protein phosphorylation is the most common type of post-translational modification in eukaryotes. The phosphoproteome is defined as the complete set of experimentally detectable phosphorylation sites present in a cell's proteome under various conditions. However, we are still far from identifying all the phosphorylation sites in a cell mainly due to the lack of information about phosphorylation events involving residues other than Ser, Thr and Tyr. Four types of phosphate-protein linkage exist and these generate nine different phosphoresidues-pSer, pThr, pTyr, pHis, pLys, pArg, pAsp, pGlu and pCys...
October 23, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28981724/dna2-initiates-resection-at-clean-dna-double-strand-breaks
#4
Sharad C Paudyal, Shan Li, Hong Yan, Tony Hunter, Zhongsheng You
Nucleolytic resection of DNA double-strand breaks (DSBs) is essential for both checkpoint activation and homology-mediated repair; however, the precise mechanism of resection, especially the initiation step, remains incompletely understood. Resection of blocked ends with protein or chemical adducts is believed to be initiated by the MRN complex in conjunction with CtIP through internal cleavage of the 5' strand DNA. However, it is not clear whether resection of clean DSBs with free ends is also initiated by the same mechanism...
November 16, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28887939/the-radiation-environment-on-the-surface-of-mars-summary-of-model-calculations-and-comparison-to-rad-data
#5
Daniel Matthiä, Donald M Hassler, Wouter de Wet, Bent Ehresmann, Ana Firan, John Flores-McLaughlin, Jingnan Guo, Lawrence H Heilbronn, Kerry Lee, Hunter Ratliff, Ryan R Rios, Tony C Slaba, Michael Smith, Nicholas N Stoffle, Lawrence W Townsend, Thomas Berger, Günther Reitz, Robert F Wimmer-Schweingruber, Cary Zeitlin
The radiation environment at the Martian surface is, apart from occasional solar energetic particle events, dominated by galactic cosmic radiation, secondary particles produced in their interaction with the Martian atmosphere and albedo particles from the Martian regolith. The highly energetic primary cosmic radiation consists mainly of fully ionized nuclei creating a complex radiation field at the Martian surface. This complex field, its formation and its potential health risk posed to astronauts on future manned missions to Mars can only be fully understood using a combination of measurements and model calculations...
August 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/28798539/acoustofluidic-bacteria-separation
#6
Sixing Li, Fen Ma, Hunter Bachman, Craig E Cameron, Xiangqun Zeng, Tony Jun Huang
Bacterial separation from human blood samples can help with the identification of pathogenic bacteria for sepsis diagnosis. In this work, we report an acoustofluidic device for label-free bacterial separation from human blood samples. In particular, we exploit the acoustic radiation force generated from a tilted-angle standing surface acoustic wave (taSSAW) field to separate E. coli from human blood cells based on their size difference. Flow cytometry analysis of the E. coli separated from red blood cells (RBCs) shows a purity of more than 96%...
January 1, 2017: Journal of Micromechanics and Microengineering: Structures, Devices, and Systems
https://www.readbyqxmd.com/read/28646879/discovery-of-a-single-male-aedes-aegypti-l-in-merseyside-england
#7
Thom Dallimore, Tony Hunter, Jolyon M Medlock, Alexander G C Vaux, Ralph E Harbach, Clare Strode
BACKGROUND: The mosquito Aedes aegypti (L.) is found in tropical and sub-tropical regions where it is the major vector of dengue fever, yellow fever, chikungunya and more recently Zika virus. Given its importance as a vector of arboviruses and its propensity to be transported to new regions, the European Centre for Disease Prevention and Control (ECDC) has placed Ae. aegypti on a list of potentially invasive mosquito species. It was previously reported in the United Kingdom (UK) in 1865 and 1919 but did not establish on either occasion...
June 24, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28630309/qnas-with-tony-hunter-and-james-allison
#8
Prashant Nair
No abstract text is available yet for this article.
June 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28604697/site-specific-incorporation-of-phosphotyrosine-using-an-expanded-genetic-code
#9
Christian Hoppmann, Allison Wong, Bing Yang, Shuwei Li, Tony Hunter, Kevan M Shokat, Lei Wang
Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination...
August 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28485115/serum-igm-level-as-predictor-of-symptomatic-hyperviscosity-in-patients-with-waldenstr%C3%A3-m-macroglobulinaemia
#10
Joshua N Gustine, Kirsten Meid, Toni Dubeau, Zachary R Hunter, Lian Xu, Guang Yang, Irene M Ghobrial, Steven P Treon, Jorge J Castillo
Symptomatic hyperviscosity is a common clinical manifestation in patients with Waldenström macroglobulinaemia (WM) and high serum IgM levels. Prompt intervention is required to prevent catastrophic events, such as retinal or central nervous system bleeding. Identifying patients at high risk of symptomatic hyperviscosity might support the decision to treat asymptomatic patients before irreversible damage occurs. We carried out a large retrospective study in 825 newly diagnosed WM patients, of who 113 (14%) developed symptomatic hyperviscosity...
June 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28461335/mechanism-of-ubiquitin-chain-synthesis-employed-by-a-hect-domain-ubiquitin-ligase
#11
Michael E French, Julian L Klosowiak, Aaron Aslanian, Steven I Reed, John R Yates, Tony Hunter
Homologous to E6AP C-terminal (HECT) ubiquitin (Ub) ligases (E3s) are a large class of enzymes that bind to their substrates and catalyze ubiquitination through the formation of a Ub thioester intermediate. The mechanisms by which these E3s assemble polyubiquitin chains on their substrates remain poorly defined. We report here that the Nedd4 family HECT E3, WWP1, assembles substrate-linked Ub chains containing Lys-63, Lys-48, and Lys-11 linkages (Lys-63 > Lys-48 > Lys-11). Our results demonstrate that WWP1 catalyzes the formation of Ub chains through a sequential addition mechanism, in which Ub monomers are transferred in a successive fashion to the substrate, and that ubiquitination by WWP1 requires the presence of a low-affinity, noncovalent Ub-binding site within the HECT domain...
June 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28432725/synthesis-and-biological-potency-of-anilino-triazine-insecticides
#12
Timothy C Johnson, Annette V Brown, Kristy K Bryan, Katherine K Guenthenspberger, Ricky Hunter, Timothy P Martin, Noormohamed Niyaz, Gene F Tisdell, Tony Trullinger
BACKGROUND: An insecticide screening effort identified N-(4-bromophenyl)-4,6-bis(2,2,2-trifluoroethoxy)-1,3,5-triazine-2-amine as having weak potency against two lepidopteran species, Helicoverpa zea and Spodoptera exigua. A structure-activity relationship study about the trifluoroethoxy substituents and the aniline of this compound was carried out in an effort to improve insecticidal potency. RESULTS: Initially, a series of analogs bearing various substituents on the aniline were prepared, and the insecticidal potency was evaluated against H...
April 22, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28235842/acquired-mutations-associated-with-ibrutinib-resistance-in-waldenstr%C3%A3-m-macroglobulinemia
#13
Lian Xu, Nicholas Tsakmaklis, Guang Yang, Jiaji G Chen, Xia Liu, Maria Demos, Amanda Kofides, Christopher J Patterson, Kirsten Meid, Joshua Gustine, Toni Dubeau, M Lia Palomba, Ranjana Advani, Jorge J Castillo, Richard R Furman, Zachary R Hunter, Steven P Treon
Ibrutinib produces high response rates and durable remissions in Waldenström macroglobulinemia (WM) that are impacted by MYD88 and CXCR4(WHIM) mutations. Disease progression can develop on ibrutinib, although the molecular basis remains to be clarified. We sequenced sorted CD19(+) lymphoplasmacytic cells from 6 WM patients who progressed after achieving major responses on ibrutinib using Sanger, TA cloning and sequencing, and highly sensitive and allele-specific polymerase chain reaction (AS-PCR) assays that we developed for Bruton tyrosine kinase (BTK) mutations...
May 4, 2017: Blood
https://www.readbyqxmd.com/read/28129587/phisphorylation-the-emergence-of-histidine-phosphorylation-as-a-reversible-regulatory-modification
#14
REVIEW
Stephen Rush Fuhs, Tony Hunter
Histidine phosphorylation is crucial for prokaryotic signal transduction and as an intermediate for several metabolic enzymes, yet its role in mammalian cells remains largely uncharted. This is primarily caused by difficulties in studying histidine phosphorylation because of the relative instability of phosphohistidine (pHis) and lack of specific antibodies and methods to preserve and detect it. The recent synthesis of stable pHis analogs has enabled development of pHis-specific antibodies and their use has started to shed light onto this important, yet enigmatic posttranslational modification...
April 2017: Current Opinion in Cell Biology
https://www.readbyqxmd.com/read/28068078/enriching-nanoparticles-via-acoustofluidics
#15
Zhangming Mao, Peng Li, Mengxi Wu, Hunter Bachman, Nicolas Mesyngier, Xiasheng Guo, Sheng Liu, Francesco Costanzo, Tony Jun Huang
Focusing and enriching submicrometer and nanometer scale objects is of great importance for many applications in biology, chemistry, engineering, and medicine. Here, we present an acoustofluidic chip that can generate single vortex acoustic streaming inside a glass capillary through using low-power acoustic waves (only 5 V is required). The single vortex acoustic streaming that is generated, in conjunction with the acoustic radiation force, is able to enrich submicrometer- and nanometer-sized particles in a small volume...
January 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/27909058/secreted-glioblastoma-nanovesicles-contain-intracellular-signaling-proteins-and-active-ras-incorporated-in-a-farnesylation-dependent-manner
#16
Natalie Luhtala, Aaron Aslanian, John R Yates, Tony Hunter
Glioblastomas (GBMs) are malignant brain tumors with a median survival of less than 18 months. Redundancy of signaling pathways represented within GBMs contributes to their therapeutic resistance. Exosomes are extracellular nanovesicles released from cells and present in human biofluids that represent a possible biomarker of tumor signaling state that could aid in personalized treatment. Herein, we demonstrate that mouse GBM cell-derived extracellular nanovesicles resembling exosomes from an H-RasV12 myr-Akt mouse model for GBM are enriched for intracellular signaling cascade proteins (GO: 0007242) and Ras protein signal transduction (GO: 0007265), and contain active Ras...
January 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27899157/disorders-of-sex-development-insights-from-targeted-gene-sequencing-of-a-large-international-patient-cohort
#17
Stefanie Eggers, Simon Sadedin, Jocelyn A van den Bergen, Gorjana Robevska, Thomas Ohnesorg, Jacqueline Hewitt, Luke Lambeth, Aurore Bouty, Ingrid M Knarston, Tiong Yang Tan, Fergus Cameron, George Werther, John Hutson, Michele O'Connell, Sonia R Grover, Yves Heloury, Margaret Zacharin, Philip Bergman, Chris Kimber, Justin Brown, Nathalie Webb, Matthew F Hunter, Shubha Srinivasan, Angela Titmuss, Charles F Verge, David Mowat, Grahame Smith, Janine Smith, Lisa Ewans, Carolyn Shalhoub, Patricia Crock, Chris Cowell, Gary M Leong, Makato Ono, Antony R Lafferty, Tony Huynh, Uma Visser, Catherine S Choong, Fiona McKenzie, Nicholas Pachter, Elizabeth M Thompson, Jennifer Couper, Anne Baxendale, Jozef Gecz, Benjamin J Wheeler, Craig Jefferies, Karen MacKenzie, Paul Hofman, Philippa Carter, Richard I King, Csilla Krausz, Conny M A van Ravenswaaij-Arts, Leendert Looijenga, Sten Drop, Stefan Riedl, Martine Cools, Angelika Dawson, Achmad Zulfa Juniarto, Vaman Khadilkar, Anuradha Khadilkar, Vijayalakshmi Bhatia, Vũ Chí Dũng, Irum Atta, Jamal Raza, Nguyen Thi Diem Chi, Tran Kiem Hao, Vincent Harley, Peter Koopman, Garry Warne, Sultana Faradz, Alicia Oshlack, Katie L Ayers, Andrew H Sinclair
BACKGROUND: Disorders of sex development (DSD) are congenital conditions in which chromosomal, gonadal, or phenotypic sex is atypical. Clinical management of DSD is often difficult and currently only 13% of patients receive an accurate clinical genetic diagnosis. To address this we have developed a massively parallel sequencing targeted DSD gene panel which allows us to sequence all 64 known diagnostic DSD genes and candidate genes simultaneously. RESULTS: We analyzed DNA from the largest reported international cohort of patients with DSD (278 patients with 46,XY DSD and 48 with 46,XX DSD)...
November 29, 2016: Genome Biology
https://www.readbyqxmd.com/read/27798325/dynact-angiography-for-the-diagnosis-of-bilateral-bow-hunter-s-syndrome
#18
Tony Lu, Ponraj Chinnadurai, Javier E Anaya-Ayala, Orlando M Diaz
We herein present a rare case of bilateral positional vertebrobasilar insufficiency secondary to mechanical obstruction of the V2 segment of the vertebral artery in a 71-year-old patient presenting with vertigo, occipital headache, tremors and respiratory irregularities. Two-dimensional digital subtraction angiography is the traditional diagnostic imaging standard but does not capture any peri-vascular bony or soft tissue abnormalities that are important to understand the three-dimensional pathophysiology. Intra-procedural cone-beam computed tomography (CT) is an increasingly used diagnostic adjunct, available in most modern angiographic suites that allows for the three-dimensional visualization of the vasculature as well as CT-like soft tissue visualization of its surrounding anatomy...
February 2017: Interventional Neuroradiology
https://www.readbyqxmd.com/read/27754503/acoustofluidic-coating-of-particles-and-cells
#19
Bugra Ayan, Adem Ozcelik, Hunter Bachman, Shi-Yang Tang, Yuliang Xie, Mengxi Wu, Peng Li, Tony Jun Huang
On-chip microparticle and cell coating technologies enable a myriad of applications in chemistry, engineering, and medicine. Current microfluidic coating technologies often rely on magnetic labeling and concurrent deflection of particles across laminar streams of chemicals. Herein, we introduce an acoustofluidic approach for microparticle and cell coating by implementing tilted-angle standing surface acoustic waves (taSSAWs) into microchannels with multiple inlets. The primary acoustic radiation force generated by the taSSAW field was exploited in order to migrate the particles across the microchannel through multiple laminar streams, which contained the buffer and coating chemicals...
November 1, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27562445/idelalisib-in-waldenstr%C3%A3-m-macroglobulinemia-high-incidence-of-hepatotoxicity
#20
Jorge J Castillo, Joshua N Gustine, Kirsten Meid, Toni Dubeau, Guang Yang, Lian Xu, Zachary R Hunter, Steven P Treon
No abstract text is available yet for this article.
August 26, 2016: Leukemia & Lymphoma
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