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https://www.readbyqxmd.com/read/28108933/modelling-genome-wide-topological-associating-domains-in-mouse-embryonic-stem-cells
#1
REVIEW
Y Zhan, L Giorgetti, G Tiana
Chromosome conformation capture (3C)-based techniques such as chromosome conformation capture carbon copy (5C) and Hi-C revealed that the folding of mammalian chromosomes is highly hierarchical. A fundamental structural unit in the hierarchy is represented by topologically associating domains (TADs), sub-megabase regions of the genome within which the chromatin fibre preferentially interacts. 3C-based methods provide the mean contact probabilities between chromosomal loci, averaged over a large number of cells, and do not give immediate access to the single-cell conformations of the chromatin fibre...
January 20, 2017: Chromosome Research
https://www.readbyqxmd.com/read/28108557/low-level-mhc-class-ii-expression-leads-to-suboptimal-th-cell-response-increased-autoaggression-and-heightened-cytokine-inducibility
#2
Yi-Ting Chen, Yu-Chia Su, Mei-Ling Chang, Pi-Fang Tsai, John T Kung
The development and activation of MHC class II (MHC-II)-restricted CD4(+) T cells are distinct immunological processes that are strictly MHC-II-dependent. To address their relative dependence on MHC-II, we established a novel ENU-induced mutant mouse on the C57BL/6 background, named I-A(12%), with ∼8-fold reduced I-A expression on the surface of B cells, dendritic cells, cortical thymic epithelial cells, and medullary thymic epithelial cells. I-A(100%) and I-A(12%) mice are highly similar with respect to the numbers of double-positive thymocytes, CD4(+)CD8(-) T cells, regulatory T cells, CD4(+) T cell marker expression, lifespan, and Th/regulatory T cell function...
January 20, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28108258/downregulation-of-iduna-is-associated-with-aif-nuclear-translocation-in-neonatal-brain-after-hypoxia-ischemia
#3
Xiaoxia Jianhua Yang Cheng, Yubo Juan Gao Ding, Xinli Ni
In adult stroke models, the neuroprotective protein, Iduna, inhibits poly (ADP-ribose) polymerase-1 (PARP-1)-dependent cell death by decreasing apoptosis-inducing factor (AIF) nuclear translocation. Because the PARP1-dependent pathway and Iduna, which promotes AIF degradation, contribute to hypoxic-ischemic (HI) brain damage in the immature brain, we examined the relationship between Iduna expression and AIF nuclear translocation in the cerebral cortex of postnatal day 7 rats after HI. Ninety rats were divided into three groups: sham, 1-h hypoxia and 2-h hypoxia...
January 17, 2017: Neuroscience
https://www.readbyqxmd.com/read/28108183/evaluation-of-multiplex-assay-platforms-for-detection-of-influenza-hemagglutinin-subtype-specific-antibody-responses-influenza-ha-subtype-specific-antibody-detection-platform
#4
Zhu-Nan Li, Kimberly M Weber, Rebecca A Limmer, Bobbi J Horne, James Stevens, Joy Schwerzmann, Jens Wrammert, Megan McCausland, Andrew J Phipps, Kathy Hancock, Daniel B Jernigan, Min Levine, Jacqueline M Katz, Joseph D Miller
Influenza hemagglutination inhibition (HI) and virus microneutralization assays (MN) are widely used for seroprevalence studies. However, these assays have limited field portability and are difficult to fully automate for high throughput laboratory testing. To address these issues, three multiplex influenza subtype-specific antibody detection assays were developed using recombinant hemagglutinin antigens in combination with Chembio, Luminex(®), and ForteBio(®) platforms. Assay sensitivity, specificity, and subtype cross-reactivity were evaluated using a panel of well characterized human sera...
January 17, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28107558/predictors-of-summertime-heat-index-levels-in-new-york-city-apartments
#5
Ashlinn Quinn, Patrick Kinney, Jeffrey Shaman
BACKGROUND: During heat waves, fatal overexposure to heat most often occurs at home. It is not known how factors such as building size, floor level, and different types of air conditioning (AC) contribute to excess indoor heat. METHODS: We monitored indoor temperature and humidity in 36 apartments in New York City during summers 2014 and 2015, and used these values to calculate the indoor heat index (HI). We investigated the role of AC type and building-level factors on indoor HI using multilevel regression models...
January 20, 2017: Indoor Air
https://www.readbyqxmd.com/read/28107166/a-new-strategy-for-calculating-the-risk-of-ovarian-malignancy-algorithm-roma
#6
Tae-Dong Jeong, Eun-Jung Cho, Dae-Hyun Ko, Woochang Lee, Sail Chun, Hi Jeong Kwon, Ki-Sook Hong, Yong-Man Kim, Won-Ki Min
BACKGROUND: Reliable quantitative measurements of HE4 and CA125 levels are required to calculate the risk of ovarian malignancy algorithm (ROMA) value. We suggest a new reporting strategy for interpreting ROMA values based on analytical measurement range (AMR) and qualified-intervals of the HE4 and CA125 results. METHODS: HE4 and CA125 assays from Abbott and Roche were used. The AMRs and the qualified-intervals were as follows: Architect HE4 assay, 20-1500 and 17...
January 20, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28106253/massive-plasmablast-response-elicited-in-the-acute-phase-of-hantavirus-pulmonary-syndrome
#7
Marina García, Ayelén Iglesias, Verónica I Landoni, Carla Bellomo, Agostina Bruno, María Teresa Córdoba, Luciana Balboa, Gabriela C Fernández, María Del Carmen Sasiain, Valeria P Martínez, Pablo Schierloh
Beside its key diagnostic value, the humoral immune response is thought to play a protective role in Hantavirus Pulmonary Syndrome (HPS). However, little is known about the cell source of these antibodies during ongoing human infection. Herein we characterized B cell subsets circulating in Andes virus-infected patients. A notable potent plasmablast (PB) response that increased 100-fold over the baseline levels was observed around one week after the onset of symptoms. These PB present a CD3(neg) CD19(low) CD20(neg) CD38(hi) CD27(hi) CD138(+/-) IgA(+/-) surface phenotype together with the presence of cytoplasmic functional immunoglobulins...
January 20, 2017: Immunology
https://www.readbyqxmd.com/read/28105895/prefrontal-cortex-dysfunction-in-hypoxic-ischaemic-encephalopathy-contributes-to-executive-function-impairments-in-rats-potential-contribution-for-attention-deficit-hyperactivity-disorder
#8
Patrícia Maidana Miguel, Bruna Ferrary Deniz, Iohanna Deckmann, Heloísa Deola Confortim, Ramiro Diaz, Daniela Pereira Laureano, Patrícia Pelufo Silveira, Lenir Orlandi Pereira
OBJECTIVES: The attention-deficit/hyperactivity disorder (ADHD) compromises the quality of life of individuals including adaptation to the social environment. ADHD aetiology includes perinatal conditions such as hypoxic-ischaemic events; preclinical studies have demonstrated attentional deficits and impulsive-hyperactive outcomes after neonatal hypoxic and/or ischaemic intervention, but data are missing to understand this relationship. Thus, the aim of this study was to evaluate executive function (EF) and impulsivity, and tissue integrity and dopaminergic function in the prefrontal cortex (PFC) of rats submitted to hypoxia-ischaemia (HI)...
January 20, 2017: World Journal of Biological Psychiatry
https://www.readbyqxmd.com/read/28105887/light-microscopy-with-a-differential-staining-technique-for-the-characterization-and-discrimination-of-insects-versus-marine-arthropods-in-processed-animal-proteins
#9
Matteo Ottoboni, Marco Tretola, Federica Cheli, Daniela Marchis, Pascal Veys, Vincent Baeten, Luciano Pinotti
The aim of this study was to evaluate the use of light microscopy with a differential staining technique for the discrimination of insect material from marine arthropods - classified as fishmeal. Specifically, three samples of single species insect material, Hermetia illucens (HI), Bombyx mori (BM) and Tenebrio molitor (TM) and two samples of marine arthropods, shrimp material and krill, have been analysed and compared after staining by two reagents to enhance fragment identification. Alizarin Red (AR) and Chlorazol black (CB), which react respectively with calcium salts and chitin, were tested for their potential efficacy in distinguishing between insect and marine materials...
January 20, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28104487/comparison-of-a-special-designed-high-intensity-coil-to-a-standard-round-coil-tms-setting
#10
Andrea Szelényi, Julia Wölfle, Igor Fischer, Hans-Jakob Steiger, Michael Sabel
BACKGROUND: Routine diagnostic Transcranial Magnetic Stimulation (TMS) is performed with a round coil (RC) for cranial and spinal root stimulation, being less successful for motor evoked potentials (MEP) of lower limb muscles. MEP elicited with a special configured flat figure-of-eight coil designed for high intensity stimulation (HI-coil) were compared to RC with regard to handling, efficiency, and physiological properties of MEP. NEW METHODS: MEP elicited with HI-coil and 9-cm diameter RC for cortical, spinal and peripheral stimulation (PES) were compared for Motor threshold (MT), latencies and amplitudes of bilateral Abductor pollicis brevis (APB) and Abductor hallucis muscles (AH)...
January 16, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28103833/prevalence-of-attention-deficit-hyperactivity-disorder-among-children-and-adolescents-in-china-a-systematic-review-and-meta-analysis
#11
Tingting Wang, Kaihua Liu, Zhanzhan Li, Yang Xu, Yuan Liu, Wenpei Shi, Lizhang Chen
BACKGROUND: Attention deficit/hyperactivity disorder (ADHD), the most common childhood neurobehavioural disorder, can produce a series of negative effects on children, adolescents, and even adults as well as place a serious economic burden on families and society. However, the prevalence of ADHD is not well understood in China. The goal of this study was to estimate the pooled prevalence of ADHD among children and adolescents in China using a systematic review and meta-analysis. METHODS: A systematic literature search was conducted in PubMed, Web of Science, MEDLINE, CNKI, Wanfang, Weipu and CBM databases, and relevant articles published from inception to March 1, 2016, that provided the prevalence of ADHD among children and adolescents in China were reviewed...
January 19, 2017: BMC Psychiatry
https://www.readbyqxmd.com/read/28101531/osteopontin-is-a-blood-biomarker-for-microglial-activation-and-brain-injury-in-experimental-hypoxic-ischemic-encephalopathy
#12
Yikun Li, Eric B Dammer, Xiaohui Zhang-Brotzge, Scott Chen, Duc M Duong, Nicholas T Seyfried, Chia-Yi Kuan, Yu-Yo Sun
Clinical management of neonatal hypoxic-ischemic encephalopathy (HIE) suffers from the lack of reliable surrogate marker tests. Proteomic analysis may identify such biomarkers in blood, but there has been no proof-of-principle evidence to support this approach. Here we performed in-gel trypsin digestion of plasma proteins from four groups of 10-d-old mice [untouched and 24 h after low-dose lipopolysaccharide (LPS) exposure, hypoxia-ischemia (HI), or LPS/HI injury; n = 3 in each group) followed by liquid chromatography-tandem mass spectrometry and bioinformatics analysis to search for HI- and LPS/HI-associated brain injury biomarkers...
January 2017: ENeuro
https://www.readbyqxmd.com/read/28100631/association-of-triiodothyronine-levels-with-future-development-of-metabolic-syndrome-in-euthyroid-middle-aged-subjects-a-6-year-retrospective-longitudinal-study
#13
Hye Jeong Kim, Ji Cheol Bae, Hyeong Kyu Park, Dong Won Byun, Kyo Il Seo, Myung Hi Yoo, Jae Hwan Jee, Jae Hyeon Kim, Yong-Ki Min, Sun Wook Kim, Jae Hoon Chung
Background Several cross-sectional studies have reported that thyroid hormone levels are associated with cardiovascular risk markers and metabolic syndrome (MetS) even in euthyroid subjects. However, the prognostic role of serum thyroid hormone levels in the risk of incident MetS has not been elucidated. Aim We aimed to investigate the associations of baseline serum thyroid hormone levels with the development of MetS in healthy subjects. Methods This 6-year, cross-sectional, longitudinal and follow-up study was conducted in 12,037 euthyroid middle-aged subjects without MetS subjected to comprehensive health examinations...
January 18, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28100621/recombinant-adenoviruses-displaying-matrix-2-ectodomain-epitopes-m2e-on-their-fiber-proteins-as-universal-influenza-vaccines
#14
Xinying Tang, Yong Yang, Xiaoli Xia, Chao Zhang, Xi Yang, Yufeng Song, Xinyi Dai, Min Wang, Dongming Zhou
: Influenza is a zoonotic disease that poses severe threats to public health and the global economy. Re-emerging influenza pandemics highlight the demand for universal influenza vaccines. We developed a novel virus platform, AdC68-F3M2e, by introducing three conserved M2e epitopes into the HI loop of the chimpanzee adenovirus (AdV) fiber protein. The M2e epitopes were expressed sufficiently on the AdV virion surface without affecting fiber trimerization. Additionally, one recombinant adenovirus, AdC68-F3M2e(H1-H5-H7), induced robust M2e-specific antibody responses in BALB/c mice after two sequential vaccinations and conferred efficient protection against homologous and heterologous IV challenges...
January 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28100496/preexisting-immunity-not-frailty-phenotype-predicts-influenza-post-vaccination-titers-among-older-veterans
#15
Puja Van Epps, Terrence Tumpey, Melissa B Pearce, Hana Golding, Patricia Higgins, Thomas Hornick, Christopher Burant, Brigid M Wilson, Richard Banks, Stefan Gravenstein, David H Canaday
Both preexisting immunity to influenza and age have been shown to be a correlate of influenza vaccine response. Frailty, an indicator of functional impairment in older adults, has also been shown in one study to predict lower influenza vaccine responses among non-veterans. In the current study we aimed to determine the association between frailty, preexisting immunity and immune response to influenza vaccine in older veterans. We analyzed 117 subjects (age range 62-95 years; median 81) divided into 3 cohorts based on the Fried frailty test: non-frail (NF: N=23; median age 68), pre-frail (PF: N=50; median age 80) and frail (F:N=44; median age 82) during the 2010-2011 and 11-12 influenza seasons...
January 18, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28100232/equitable-health-services-for-the-young-a-decomposition-of-income-related-inequalities-in-young-adults-utilization-of-health-care-in-northern-sweden
#16
Paola A Mosquera, Anna-Karin Waenerlund, Isabel Goicolea, Per E Gustafsson
BACKGROUND: Despite the goal of the Swedish health system to offer health care according to the principle of horizontal equity, little is known about the equality in access to health care use among young people. To explore this issue, the present study aimed i) to assess horizontal inequity in health care utilization among young people in Northern Sweden; and ii) to explore the contribution of different factors to explain the observed inequalities. METHODS: Participants (N = 3016 youths aged 16-25 years) came from the "Health on Equal terms" survey conducted in 2014 in the four northernmost counties in Sweden...
January 18, 2017: International Journal for Equity in Health
https://www.readbyqxmd.com/read/28099999/high-level-intrathymic-thyrotropin-receptor-expression-in-thyroiditis-prone-mice-protects-against-the-spontaneous-generation-of-pathogenic-thyrotropin-receptor-autoantibodies
#17
Sandra M McLachlan, Holly A Aliesky, Bianca Banuelos, Sylvie Lesage, Roxanne Collin, Basil Rapoport B
The thyrotropin receptor (TSHR) A-subunit is the autoantigen targeted by pathogenic autoantibodies that cause Graves' hyperthyroidism, a common autoimmune disease in humans. Previously, we reported that pathogenic TSHR antibodies develop spontaneously in thyroiditis-susceptible NOD.H2(h4) mice bearing a human TSHR A-subunit transgene which is expressed at low levels in both the thyroid and thymus (Lo-expressor transgene). The present study tested recent evidence that high intrathymic TSHR expression protects against the development of pathogenic TSHR antibodies in humans...
January 18, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28098800/characterization-of-the-transcriptome-and-gene-expression-of-brain-tissue-in-sevenband-grouper-hyporthodus-septemfasciatus-in-response-to-nnv-infection
#18
Jong-Oh Kim, Jae-Ok Kim, Wi-Sik Kim, Myung-Joo Oh
Grouper is one of the favorite sea food resources in Southeast Asia. However, the outbreaks of the viral nervous necrosis (VNN) disease due to nervous necrosis virus (NNV) infection have caused mass mortality of grouper larvae. Many aqua-farms have suffered substantial financial loss due to the occurrence of VNN. To better understand the infection mechanism of NNV, we performed the transcriptome analysis of sevenband grouper brain tissue, the main target of NNV infection. After artificial NNV challenge, transcriptome of brain tissues of sevenband grouper was subjected to next generation sequencing (NGS) using an Illumina Hi-seq 2500 system...
January 13, 2017: Genes
https://www.readbyqxmd.com/read/28098157/antibody-responses-to-trivalent-inactivated-influenza-vaccine-in-health-care-personnel-previously-vaccinated-and-vaccinated-for-the-first-time
#19
Kuan-Ying A Huang, Shih-Cheng Chang, Yhu-Chering Huang, Cheng-Hsun Chiu, Tzou-Yien Lin
Inactivated influenza vaccination induces a hemagglutinin-specific antibody response to the strain used for immunization. Annual vaccination is strongly recommended for health care personnel. However, it is debatable if repeated vaccination would affect the antibody response to inactivated influenza vaccine through the time. We enrolled health care personnel who had repeated and first trivalent inactivated influenza vaccination in 2005-2008. Serological antibody responses were measured by hemagglutination-inhibition (HI) test...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28095321/cellulose-to-depolymerize%C3%A2-or-not-to
#20
REVIEW
Sergiu Coseri
Oxidation of the primary OH groups in cellulose is a pivotal reaction both at lab and industrial scale, leading to the value-added products, i.e. oxidized cellulose which have tremendous applications in medicine, pharmacy and hi-tech industry. Moreover, the introduction of carboxyl moieties creates prerequisites for further cellulose functionalization through covalent attachment or electrostatic interactions, being an essential achievement designed to boost the area of cellulose-based nanomaterials fabrication...
January 14, 2017: Biotechnology Advances
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