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Semen Analysis

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https://www.readbyqxmd.com/read/28938737/complex-catsper-dependent-and-independent-ca2-i-signalling-in-human-spermatozoa-induced-by-follicular-fluid
#1
Sean G Brown, Sarah Costello, Mark C Kelly, Mythili Ramalingam, Ellen Drew, Stephen J Publicover, Christopher L R Barratt, Sarah Martins Da Silva
STUDY QUESTION: Does progesterone in human follicular fluid (hFF) activate CatSper and do other components of hFF modulate this effect and/or contribute separately to hFF-induced Ca2+ signaling? SUMMARY ANSWER: hFF potently stimulates CatSper and increases [Ca2+]i, primarily due to high concentrations of progesterone, however, other components of hFF also contribute to [Ca2+]i signaling, including modulation of CatSper channel activity and inhibition of [Ca2+]i oscillations...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28933115/-rancidness-of-armeniacae-semen-amarum-involving-bianzhuang-lunzhi
#2
Jian-Ting Gong, Li-Ying Zhao, Bauer Rudolf, Wen-Juan Mi, Yang Li, Jia-Hui Li, Zhi-Yu Ren, Dong Xu, Ting Zhao, Yong-Hong Yan
This article aims to compare the qualities of Armeniacae Semen Amarum before and after rancidness, in order to study the rancidness of Armeniacae Semen Amarum. In the experiment, content of fatty oil, acid value and peroxide value were determined before and after rancidness,respectively. Meanwhile, HPLC, GC-MS were utilized to analyze laetrile and fatty acid components. Besides, colorimeter and e-nose were introduced to quantify and compare "color and odor". A correlation analysis was conducted on the above results...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28929253/positive-rheotaxis-extended-drop-a-one-step-procedure-to-select-and-recover-sperm-with-mature-chromatin-for-intracytoplasmic-sperm-injection
#3
Hamilton De Martin, Marcello S Cocuzza, Bruno C Tiseo, Guilherme J A Wood, Eduardo P Miranda, Pedro A A Monteleone, José Maria Soares, Paulo C Serafini, Miguel Srougi, Edmund C Baracat
PURPOSE: The purpose of this study was to develop a novel one-step ICSI approach to select sperm with better chromatin maturity than the conventional method. METHODS: This was a pilot diagnostic study, which prospectively recruited men during a 6-month period in a University-affiliated infertility centre. Forty consecutive semen samples were provided for analysis. The positive rheotaxis extended drop (PRED) was set up creating a pressure and viscosity gradient. Each semen sample was divided into four aliquots: one aliquot for density gradient centrifugation (DGC), two aliquots for PRED (fresh semen (PRED-FS) and processed semen (PRED-DGC)), and one aliquot as the control (FS)...
September 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28920796/successful-laparoscopic-oviductal-artificial-insemination-in-the-clouded-leopard-neofelis-nebulosa-in-thailand
#4
Wanlaya Tipkantha, Paweena Thuwanut, Umaporn Maikeaw, Ampika Thongphakdee, Saifon Yapila, Sumate Kamolnorranath, Boripat Siriaroonrat, Pierre Comizzoli, Kaywalee Chatdarong
Captive breeding of clouded leopards (Neofelis nebulosa) is challenging because of mating incompatibility, high incidence of teratospermia in males, and inconsistent ovulation patterns in females. Assisted reproductive techniques, therefore, are necessary to overcome these issues and maintain the genetic diversity in the captive population. The objective was to use laparoscopic oviductal artificial insemination (AI) to breed genetically valuable females (n = 4; aged 4.5-5 yr) that were unsuccessfully paired...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28916146/chinese-sacbrood-virus-infection-in-asian-honey-bees-apis-cerana-cerana-and-host-immune-responses-to-the-virus-infection
#5
Liu Shan, Wang Liuhao, Guo Jun, Tang Yujie, Chen Yanping, Wu Jie, Li Jilian
Chinese Sacbrood virus (CSBV) is a positive-stranded RNAvirus that infects both the European honey bee (Apis mellifera) and the Asian honey bee (A. cerana). However, CSBV has much more devastating effects on Asian honey bees than on European honey bees, posing a serious threat to the agricultural and natural ecosystems that rely on A. cerana for pollination service. Using quantitative RT-PCR method, we conducted studies to examine the CSBV infection in Asian honey bee colonies and immune responses of individual bees in response to CSBV infection...
September 12, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28911211/are-there-intracellular-ca2-oscillations-correlated-with-flagellar-beating-in-human-sperm-a-three-vs-two-dimensional-analysis
#6
G Corkidi, F Montoya, P Hernández-Herrera, W A Ríos-Herrera, M F Müller, C L Treviño, A Darszon
STUDY QUESTION: Are there intracellular Ca2+ ([Ca2+]i) oscillations correlated with flagellar beating in human sperm? SUMMARY ANSWER: The results reveal statistically significant [Ca2+]i oscillations that are correlated with the human sperm flagellar beating frequency, when measured in three-dimensions (3D). WHAT IS KNOWN ALREADY: Fast [Ca2+]i oscillations that are correlated to the beating flagellar frequency of cells swimming in a restricted volume have been detected in hamster sperm...
September 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28892594/male-infertility-the-other-side-of-the-equation
#7
Darren J Katz, Patrick Teloken, Ohad Shoshany
BACKGROUND: A male factor contributes to infertility in approximately 50% of couples who fail to conceive, causing significant psychosocial and marital stress. OBJECTIVE: This article reviews the general practitioner's (GP's) evaluation of male infertility and indications for referral to a male infertility specialist, and gives an overview of the specialist management of male infertility. DISCUSSION: Male infertility can result from anatomical or genetic abnormalities, systemic or neurological diseases, infections, trauma, iatrogenic injury, gonadotoxins and development of sperm antibodies...
September 2017: Australian Family Physician
https://www.readbyqxmd.com/read/28891798/-effect-of-the-cellular-and-humoral-immune-response-in-infertile-couples-and-its-relationship-with-infections
#8
Adriana Brufman, Laura Colombo, Esteban Streiger, Rocío Pusillico
OBJECTIVE: The purpose of this study was to determine the prevalence of different microorganisms isolated from the urogenital tract of infertile men and assess whether there are differences in semen parameters according to the presence or absence of genital infections. METHODS: 280 semen samples from infertile men were studied retrospectively. Semen parameters were analyzed according to the World Health Organization criteria (WHO 2010). Microbiological analysis was performed using the modified method of Stamey and Meares proposed by Santoianni et al...
September 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28891229/effect-of-different-mini-volume-colloid-centrifugation-configurations-on-flow-cytometrically-sorted-sperm-recovery-efficiency-and-quality-using-a-computer-assisted-semen-analyzer
#9
L B Ferré, J L Chitwood, C Fresno, H H Ortega, M E Kjelland, P J Ross
Straws of sex-sorted sperm are usually packaged at a low concentration (e.g., ~2.1 × 10(6)  sperm/ml) and cost significantly more than unsorted conventional semen from the same sire. In order to maximize the efficiency of using sex-sorted sperm under in vitro fertilization conditions, the selection of an appropriate sperm separation technique is essential. In this study, the effect of using different silane-coated silica colloid dilutions and layering configurations during centrifugation of sex-sorted sperm was examined over an extended period of incubation time...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28891125/effect-of-cryoprotectants-and-thawing-temperatures-on-chicken-sperm-quality
#10
M Miranda, B Kulíková, J Vašíček, L Olexiková, N Iaffaldano, P Chrenek
There is need for standardization of freezing-thawing protocol for rooster semen to minimize variability among results. Therefore, we aimed to compare effect of four different permeating cryoprotectants and two thawing temperatures (37 vs. 5°C) on sperm post-thaw motility and to analyse combined effect of the best permeating cryoprotectant (P-CPA) with one of four non-permeating cryoprotectants (N-CPA) on post-thaw quality of rooster semen evaluated in vitro. Pooled semen from Ross PM3 rooster heavy line was diluted in Kobidil extender and frozen in cryoprotectant solution containing 6% dimethylacetamide, 7...
September 11, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28890972/nanomechanical-sensors-for-direct-and-rapid-characterization-of-sperm-motility-based-on-nanoscale-vibrations
#11
Shangquan Wu, Zhiguo Zhang, Xiarong Zhou, Hong Liu, Changguo Xue, Gang Zhao, Yunxia Cao, Qingchuan Zhang, Xiaoping Wu
Infertility, whether of male or female origin, is a critical challenge facing the low birth rate and aging population throughout the world, and semen analysis is a cornerstone of the diagnostic evaluation of the male contribution to infertility. This means that tools which can characterize sperm properties in an effective manner are very much needed. The conventional approaches are essentially image-based, which have a limited value for analyzing sperm properties. Here, we show that an assay using nanomechanical sensors can detect sperm motility based on nanomotion...
September 11, 2017: Nanoscale
https://www.readbyqxmd.com/read/28886318/new-insights-into-the-expression-profile-of-microrna-34c-and-p53-in-infertile-men-spermatozoa-and-testicular-tissue
#12
S Rahbar, M G Novin, E Alizadeh, V Shahnazi, F Pashaei-Asl, Y A AsrBadr, L Farzadi, E Ebrahimie, M Pashaiasl
Spermatogenesis is proliferation and differentiation processes of stem spermatogonia into mature spermatozoa controlled by the genes responsible for transcription and post transcription levels. MicroRNAs (miRNA) are  the key factors during gene expression in RNA silencing and post-transcriptional regulation. They play main roles in regulation of early and late spermatogenesis, and reproduction. In this study, we investigate the role of miRNAs in infertile males.The patients were assigned to five groups based on semen analysis (n=55), including normozoospermic (N), moderate oligoasthenoteratozoospermic (MOAT), severe oligoasthenoteratozoospermic (SOAT), obstructive azoospermia (OA) and non-obstructive azoospermia (NOA)...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28869111/enhancement-of-sperm-motility-and-viability-by-turmeric-by-product-dietary-supplementation-in-roosters
#13
Wenjing Yan, Chihiro Kanno, Eiki Oshima, Yukiko Kuzuma, Sung Woo Kim, Hanako Bai, Masashi Takahashi, Yojiro Yanagawa, Masashi Nagano, Jun-Ichi Wakamatsu, Manabu Kawahara
Improving sperm motility and viability are major goals to improve efficiency in the poultry industry. In this study, the effects of supplemental dietary turmeric by-product (TBP) from commercial turmeric production on sperm motility, viability, and antioxidative status were examined in domestic fowl. Mature Rhode Island Red roosters were divided into two groups - controls (groupC) without TBP administration and test subjects (groupT) fed a basal diet supplemented with 0.8g of TBP/day in a temperature-controlled rearing facility (Experiment 1) and 1...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28868837/physically-active-men-show-better-semen-parameters-than-their-sedentary-counterparts
#14
Paula C Lalinde-Acevedo, B Jose Manuel Mayorga-Torres, Ashok Agarwal, Stefan S du Plessis, Gulfam Ahmad, Ángela P Cadavid, Walter D Cardona Maya
Background: The quality of semen depends upon several factors such as environment, life style, physical activity, age, and occupation. The aim of this study was to analyze and compare the conventional and functional semen parameters in men practicing vigorous physical activity to those of sedentary men. MATERIALS AND METHODS: In this descriptive cross-sectional study, semen samples of 17 physically active men and 15 sedentary men were collected for analysis. Semen analysis was performed according to the World Health Organization (WHO) guidelines, while functional parameters were evaluated by flow cytometry...
October 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28867410/boar-variability-in-sperm-cryo-tolerance-after-cooling-of-semen-in-different-long-term-extenders-at-various-temperatures
#15
K Wasilewska, L Fraser
This study investigated individual boar variability in the quality of pre-freeze (PF) and post-thaw (PT) semen cooled in different long-term (LT) extenders and for different holding times (HT). Sperm rich fractions were diluted with Androhep(®) Plus (AHP), Androstar(®) Plus (ASP), Safecell(®) Plus (SCP) and TRIXcell(®) Plus (TCP) extenders, stored for 2h at 17°C (HT 1) and additionally for 24h at 10°C (HT 2) and the samples were subsequently evaluated and frozen. Besides the analysis of CASA sperm variables, mitochondrial membrane potential (MMP), plasma membrane integrity (PMI), normal apical ridge (NAR) acrosome integrity, and viability (YO-PRO-1(-)/PI(-)) of sperm were assessed in the PF and PT semen...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28865553/motivations-and-attitudes-of-candidate-sperm-donors-in-belgium
#16
Annelies Thijssen, Veerle Provoost, Eva Vandormael, Nathalie Dhont, Guido Pennings, Willem Ombelet
OBJECTIVE: To study the motivation and attitudes of a candidate sperm donor population in Belgium. DESIGN: Anonymous survey. SETTING: Tertiary referral infertility center. PATIENT(S): One hundred candidate sperm donors applying to the center for a first semen analysis between April 2013 and March 2016. INTERVENTION(S): Invitation to complete an anonymous questionnaire. MAIN OUTCOME MEASURE(S): Demographic characteristics, recruitment methods, motivations and attitudes toward payment, donor anonymity, disclosure to offspring, donation to lesbian couples and single women, views on the donor children and social aspects of sperm donation...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28865546/reproductive-outcomes-of-testicular-versus-ejaculated-sperm-for-intracytoplasmic-sperm-injection-among-men-with-high-levels-of-dna-fragmentation-in-semen-systematic-review-and-meta-analysis
#17
Sandro C Esteves, Matheus Roque, Cara K Bradley, Nicolás Garrido
OBJECTIVE: To compare sperm DNA fragmentation (SDF) levels between testicular and ejaculated sperm and to evaluate outcomes of intracytoplasmic sperm injection (ICSI) with the use of testicular (Testi-ICSI) versus ejaculated (Ejac-ICSI) sperm in nonazoospermic men with high SDF. DESIGN: Systematic review and meta-analysis. SETTING: Not applicable. PATIENT(S): Normo- and oligozoospermic men with high levels of SDF in semen subjected to Testi-ICSI or Ejac-ICSI...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28861318/digeorge-syndrome-associated-with-azoospermia-first-case-in-the-literature
#18
Ayşegül Özcan, Yavuz Şahin
DiGeorge syndrome (DGS) is one of the most frequently seen chromosomal abnormalities. The major genetic cause of DGS is a microdeletion on chromosome 22q11.2. Majority of the cases are diagnosed during their childhood. DGS is rarely considered and diagnosed in adulthood. Herein, we report the first case of a patient with DGS and azoospermia in the literature. Our patient was a 35-year-old male with mild dysmorphic features, hypernasal voice, mental retardation, and azoospermia. His laboratory tests and echocardiographic assessments were normal...
September 2017: Turkish Journal of Urology
https://www.readbyqxmd.com/read/28859153/clonality-and-distribution-of-clinical-ureaplasma-isolates-recovered-from-male-patients-and-infertile-couples-in-china
#19
Zhi Ruan, Ting Yang, Xinyan Shi, Yingying Kong, Xinyou Xie, Jun Zhang
Ureaplasma spp. have gained increasing recognition as pathogens in both adult and neonatal patients with multiple clinical presentations. However, the clonality of this organism in the male population and infertile couples in China is largely unknown. In this study, 96 (53 U. parvum and 43 U. urealyticum) of 103 Ureaplasma spp. strains recovered from genital specimens from male patients and 15 pairs of infertile couples were analyzed using multilocus sequence typing (MLST)/expanded multilocus sequence typing (eMLST) schemes...
2017: PloS One
https://www.readbyqxmd.com/read/28856695/association-between-body-mass-index-and-sperm-quality-and-sperm-dna-integrity-a-large-population-study
#20
J B A Oliveira, C G Petersen, A L Mauri, L D Vagnini, A Renzi, B Petersen, M Mattila, F Dieamant, R L R Baruffi, J G Franco
This study aimed to analyse whether the functional quality of spermatozoa is associated with body mass index (BMI). Semen samples were obtained from 1824 men undergoing fertility evaluation/treatment. Semen analysis was performed using World Health Organization (WHO) criteria, and morphology was evaluated with the motile sperm organelle morphology examination (MSOME). The percentages of sperm DNA fragmentation (using TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick-end labelling (TUNEL) assays), sperm chromatin packaging/underprotamination (using chromomycin A3/CMA3 ), mitochondrial damage (using MitoTracker Green) and apoptosis (using annexin V) were also assessed...
August 30, 2017: Andrologia
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