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Semen Analysis

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https://www.readbyqxmd.com/read/29144025/detection-of-atypical-porcine-pestivirus-in-semen-from-commercial-boar-studs-in-the-united-states
#1
I R H Gatto, P H Arruda, C A Visek, J G Victoria, A R Patterson, A C Krull, K J Schwartz, L G de Oliveira, B L Arruda
Atypical porcine pestivirus (APPV) has recently been identified as a cause of congenital tremor (CT) in pigs and has been detected in semen and preputial swabs from boars that were known to be clinically affected with CT. Accordingly, the objectives of this study were to 1) detect the presence of APPV in semen, preputial fluids and preputial swabs from adult boars by quantitative reverse transcription PCR (qRT-PCR) and 2) genetically characterize a subset of positive samples to better understand the ecology of APPV in commercial boar studs and the potential risk of transmission of APPV via semen...
November 16, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29143943/the-impact-of-ejaculatory-abstinence-on-semen-analysis-parameters-a-systematic-review
#2
REVIEW
Brent M Hanson, Kenneth I Aston, Tim G Jenkins, Douglas T Carrell, James M Hotaling
OBJECTIVE: The aim of this study was to evaluate recent publications and determine the impact of ejaculatory abstinence on semen analysis parameters as well as fertility outcomes. METHODS: This was a systematic review of 28 recent publications. The focus of this study was the impact of abstinence on semen parameters and fertility outcomes in papers published since the year 2000. The specific parameters evaluated were volume, sperm count, motility, morphology, pH, DNA fragmentation rate, viability, and pregnancy or fertilization rates following assisted reproduction...
November 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29143242/comparing-reactive-oxygen-species-and-dna-fragmentation-in-semen-samples-of-unexplained-infertile-and-healthy-fertile-men
#3
Zahra Zandieh, Akram Vatannejad, Mahmood Doosti, Sara Zabihzadeh, Mahnaz Haddadi, Leila Bajelan, Batool Rashidi, Saeid Amanpour
BACKGROUND: Male factor infertility has increased to more than 40% during the last decade. About 30% of these couples are diagnosed with unexplained infertility. In fact, reactive oxygen species (ROS), especially superoxide anion (O2(-)·) and hydrogen peroxide (H2O2), play a crucial role in regulation of physiological and pathological processes in spermatozoa. Moreover, since the diagnosis of unexplained infertility just through semen analysis is a matter of much controversy; we aimed to evaluate the levels of ROS and sperm DNA fragmentation in the semen samples of unexplained infertile and fertile control couples...
November 15, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29136143/time-varying-cycle-average-and-daily-variation-in-ambient-air-pollution-and-fecundability
#4
Carrie J Nobles, Enrique F Schisterman, Sandie Ha, Germaine M Buck Louis, Seth Sherman, Pauline Mendola
STUDY QUESTION: Does ambient air pollution affect fecundability? SUMMARY ANSWER: While cycle-average air pollution exposure was not associated with fecundability, we observed some associations for acute exposure around ovulation and implantation with fecundability. WHAT IS KNOWN ALREADY: Ambient air pollution exposure has been associated with adverse pregnancy outcomes and decrements in semen quality. STUDY DESIGN, SIZE, DURATION: The LIFE study (2005-2009), a prospective time-to-pregnancy study, enrolled 501 couples who were followed for up to one year of attempting pregnancy...
November 10, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29127940/deciphering-the-chemical-origin-of-the-semen-like-floral-scents-in-three-angiosperm-plants
#5
Xiaoping Zhang, Konstantin Chingin, Dacai Zhong, Liping Luo, Vladimir Frankevich, Huanwen Chen
The chemical origin and biological role of distinct semen-like odor occasionally found in some flowers are very curious but remain scarcely studied. Here, we used direct ambient corona discharge ionization mass spectrometry (MS) to study the volatile chemical composition behind the semen-like odor emitted by the fresh flowers of Photinia serrulata, Castanopsis sclerophylla and Stemona japonica without any chemical pretreatment. Chemical identification was performed using high-resolution MS analysis in combination with tandem MS analysis and whenever possible was confirmed by the analysis of standard reference compounds...
November 8, 2017: Phytochemistry
https://www.readbyqxmd.com/read/29126865/vitrification-of-dog-spermatozoa-effects-of-two-cryoprotectants-sucrose-or-trehalose-and-two-warming-procedures
#6
E Caturla-Sánchez, M J Sánchez-Calabuig, J F Pérez-Gutiérrez, J Cerdeira, C Castaño, J Santiago-Moreno
Sperm vitrification is a low cost and simple technique that does not require special equipment and may represent an attractive alternative to the costly and time consuming conventional dog spermatozoa cryopreservation techniques. The objective of this study was to evaluate different cryoprotectants and warming temperatures on the vitrification of dog spermatozoa. Pooled semen samples from 10 beagle dogs were vitrified with four extenders, based on Tris, citric acid and glucose, 20% egg yolk (TCG-20% EY) and different combinations of sucrose and/or trehalose: 250 mM sucrose; 250 mM trehalose; 125 mM sucrose + 125 mM trehalose; 250 mM sucrose + 250 mM trehalose...
November 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/29124782/the-effect-of-cigarette-smoking-on-human-seminal-parameters-sperm-chromatin-structure-and-condensation
#7
R M Mostafa, Y S Nasrallah, M M Hassan, A F Farrag, A Majzoub, A Agarwal
Considerable debate still exists regarding the effects of cigarette smoking on male fertility. This work aimed to explore effects of cigarette smoking on semen parameters and DNA fragmentation on 95 infertile patients who were divided into infertile male nonsmokers (45) and infertile male smokers (50). Smokers were subdivided according to a number of cigarettes smoked per day into mild (≤10), moderate (11-20) and heavy smokers (≥21). Semen analysis, sperm chromatin condensation integrity with aniline blue staining and sperm viability were compared between the study groups...
November 9, 2017: Andrologia
https://www.readbyqxmd.com/read/29121862/human-papillomavirus-in-semen-and-the-risk-for-male-infertility-a-systematic-review-and-meta-analysis
#8
Zhangyan Lyu, Xiaoshuang Feng, Ni Li, Wei Zhao, Luopei Wei, Yuheng Chen, Wenjing Yang, Hongxia Ma, Bing Yao, Kai Zhang, Zhibin Hu, Hongbing Shen, Dong Hang, Min Dai
BACKGROUND: Human papillomavirus (HPV) is one of the most prevalent sexually transmitted viruses. Despite the increasing evidence of HPV prevalence in semen, the worldwide distribution of HPV types in semen and risk for male infertility remain inconclusive. METHODS: Four electronic databases were searched for English language studies conducted between January 1990 and December 2016 that reported HPV DNA prevalence in semen. Based on the PRISMA guidelines, HPV prevalence was estimated among general population and fertility clinic attendees, respectively, and heterogeneity testing was performed using Cochran's Q and I (2) statistics...
November 9, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29119858/eimeria-taggarti-n-sp-a-novel-coccidian-apicomplexa-eimeriorina-in-the-prostate-of-an-antechinus-flavipes
#9
Jemima Amery-Gale, Joanne Maree Devlin, Liliana Tatarczuch, David Augustine Taggart, David J Schultz, Jenny A Charles, Ian Beveridge
A novel coccidian species was discovered in the prostate of an Antechinus flavipes (yellow-footed antechinus) in South Australia, during the period of post-mating male antechinus immunosuppression and mortality. This novel coccidian is unusual because it develops extra-intestinally and sporulates endogenously within the prostate gland of its mammalian host. Histological examination of prostatic tissue revealed dense aggregations of spherical and thin-walled tetrasporocystic, dizoic sporulated coccidian oocysts within tubular lumina, with unsporulated oocysts and gamogonic stages within the cytoplasm of glandular epithelial cells...
November 10, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/29117433/inter-and-intra-laboratory-standardization-of-tunel-assay-for-assessment-of-sperm-dna-fragmentation
#10
Sajal Gupta, Rakesh Sharma, Ashok Agarwal
The functional aspects of sperm activity such as sperm chromatin integrity and ability to fertilize cannot be characterized by routine semen parameters. Men with unexplained infertility and idiopathic infertility, as well as men with normozoospermic semen profiles, show high DNA fragmentation. Molecular anomalies in the sperm can be detected by a sperm DNA fragmentation (SDF) assay which can be used in adjunct to conventional semen analysis. While the sperm chromatin structure assay (SCSA) remains the "gold standard," the TUNEL assay using flow cytometry is becoming popular among the different tests that are currently available to measure sperm DNA fragmentation...
November 8, 2017: Current Protocols in Toxicology
https://www.readbyqxmd.com/read/29115996/serological-evidence-of-equine-arteritis-virus-infection-and-phylogenetic-analysis-of-viral-isolates-in-semen-of-stallions-from-serbia
#11
Sava Lazić, Diana Lupulović, Delphine Gaudaire, Tamas Petrovic, Gospava Lazić, Aymeric Hans
BACKGROUND: Equine arteritis virus (EAV) is responsible for infections in equids. It can spread easily within the horse population and has a major impact on the horse breeding industry. No EAV outbreak has ever been reported in Serbia. To determine whether EAV is nonetheless circulating there, especially in the Vojvodina region, 340 horse serum samples were subjected to serology testing to detect EAV antibodies. In parallel, semen samples from three seropositive stallions were collected to evaluate their EAV status, using RT-qPCR and virus isolation on cell culture...
November 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29110319/effects-of-chlamydia-trachomatis-infection-on-sperm-chromatin-condensation-and-dna-integrity
#12
L Dehghan Marvast, A R Talebi, J Ghasemzadeh, A Hosseini, A A Pacey
The present study was performed to investigate the relation of Chlamydia trachomatis infection to sperm chromatin/DNA integrity in a population of infertile men (male partner of infertile couples) from Iran. Blood, semen and first-void urine samples were obtained from 250 infertile men. Data were analysed with regard to the results of (i) serological analysis for specific antibodies to C. trachomatis in serum; (ii) the presence of C. trachomatis and DNA in first-void urine; and (iii) in the semen sample of the male partner, in addition to sperm analysis, four different tests (aniline blue, chromomycin A3, acridine orange and TUNEL) were used to detect sperm chromatin and DNA abnormalities...
November 6, 2017: Andrologia
https://www.readbyqxmd.com/read/29108863/alterations-in-sperm-dna-methylation-patterns-of-oligospermic-males
#13
Mohammed Laqqan, Sascha Tierling, Yaser Alkhaled, Christina LoPorto, Mohamad Eid Hammadeh
Aberrant in sperm DNA methylation patterns and histone modification play a role in male fecundity decline. This study was prepared to determine whether sperm DNA methylation at CpG dinucleotides is different in oligospermic males compared to proven fertile males and then to evaluate the correlation between the changes in sperm DNA methylation patterns and semen parameters of oligospermic males. A total of 165 males (64 proven fertile males "controls" and 101 oligospermic males "cases") were included in the study...
November 3, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29108030/genetic-parameters-for-semen-quality-and-quantity-traits-in-five-pig-lines
#14
D B D Marques, M S Lopes, M L W J Broekhuijse, S E F Guimarães, E F Knol, J W M Bastiaansen, F F Silva, P S Lopes
We aimed to estimate genetic parameters for semen quality and quantity traits as well as for within-boar variation of these traits to evaluate their inclusion in breeding goals. Genetic parameters were estimated within line using a multiple-trait (4 × 4) repeatability animal model fitted for 5 pig lines, considering 4 semen traits: sperm motility (MOT), sperm progressive motility (PROMOT), log-transformed number of sperm cells per ejaculate (lnN), and total morphological abnormalities (ABN). The within-boar variation of these traits was analyzed based on a multiple-trait (2 × 2) approach for SD and average (AVG) and a single-trait analysis for CV...
October 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/29102416/hair-mercury-hg-levels-fish-consumption-and-semen-parameters-among-men-attending-a-fertility-center
#15
Lidia Mínguez-Alarcón, Myriam C Afeiche, Paige L Williams, Mariel Arvizu, Cigdem Tanrikut, Chitra J Amarasiriwardena, Jennifer B Ford, Russ Hauser, Jorge E Chavarro
General population exposure to methylmercury (MeHg), the most common organic mercury compound found in the environment, occurs primarily through the consumption of contaminated fish and shellfish. Due to limited studies and lack of consideration of effect modification by fish consumption, it remains uncertain if exposure to mercury affects semen parameters. Thus, we investigated whether hair Hg levels, a biomarker of mercury exposure, were associated with semen parameters among men attending an academic fertility center, and whether this relationship was modified by intake of fish...
October 28, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29102407/physical-and-kinematic-properties-of-cryopreserved-camel-sperm-after-elimination-of-semen-viscosity-by-different-techniques
#16
Khalid El-Bahrawy, Sherif Rateb, Marwa Khalifa, Davide Monaco, Giovanni Lacalandra
This investigation aimed to determine the influence of using different techniques for liquefaction of semen on post-thaw physical and dynamic characteristics of camel spermatozoa. A total of 144 ejaculates were collected from 3 adult camels, Camelus dromedarius, twice-weekly over 3 consecutive breeding seasons. A raw aliquot of each ejaculate was evaluated for physical and morphological properties, whereas the remaining portion was diluted (1:3) with glycerolated Tris lactose egg yolk extender, and was further subjected to one of the following liquefaction treatments: control (untreated), 5μl/ml α-amylase, 0...
October 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29100112/a-dictionary-learning-approach-for-human-sperm-heads-classification
#17
Fariba Shaker, S Amirhassan Monadjemi, Javad Alirezaie, Ahmad Reza Naghsh-Nilchi
BACKGROUND AND OBJECTIVE: To diagnose infertility in men, semen analysis is conducted in which sperm morphology is one of the factors that are evaluated. Since manual assessment of sperm morphology is time-consuming and subjective, automatic classification methods are being developed. Automatic classification of sperm heads is a complicated task due to the intra-class differences and inter-class similarities of class objects. In this research, a Dictionary Learning (DL) technique is utilized to construct a dictionary of sperm head shapes...
October 10, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29089604/microfluidics-for-sperm-analysis-and-selection
#18
REVIEW
Reza Nosrati, Percival J Graham, Biao Zhang, Jason Riordon, Alexander Lagunov, Thomas G Hannam, Carlos Escobedo, Keith Jarvi, David Sinton
Infertility is a growing global health issue with far-reaching socioeconomic implications. A downward trend in male fertility highlights the acute need for affordable and accessible diagnosis and treatment. Assisted reproductive technologies are effective in treating male infertility, but their success rate has plateaued at ∼33% per cycle. Many emerging opportunities exist for microfluidics - a mature technology in other biomedical areas - in male infertility diagnosis and treatment, and promising microfluidic approaches are under investigation for addressing male infertility...
October 31, 2017: Nature Reviews. Urology
https://www.readbyqxmd.com/read/29082953/despite-limitations-sperm-dna-fragmentation-testing-provides-unique-information-complementary-to-but-distinct-from-semen-analysis-results
#19
EDITORIAL
Sandro C Esteves, Ahmad Majzoub, Ashok Agarwal
No abstract text is available yet for this article.
September 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/29082368/impact-of-age-on-semen-parameters-in-male-partners-of-infertile-couples-in-a-rural-tertiary-care-center-of-central-india-a-cross-sectional-study
#20
Naina Kumar, Amit K Singh, Ajay R Choudhari
BACKGROUND: High rates of sub-fertility and adverse pregnancy outcomes were seen after age 40. In contrast to oogenesis, spermatogenesis continues in elderly men. OBJECTIVE: To retrospectively study the impact of aging on semen parameters in male partners of infertile couples in the rural area of developing country over 10 years and to find out whether aging affects male factor fertility and various semen parameters in this part of developing country. MATERIALS AND METHODS: In this cross sectional study, the laboratory semen analysis records of 1219 male partners of infertile couples of a rural tertiary care center of Central India in a 10-year period from January 2005 to December 2014 were evaluated into 5 groups based on men age: Group 1: 21-28 yr (n=57); group 2: 29-35 yr (n=450); group 3: 36-42 yr (n=532); group 4: 43-49 yr (n=165), and group 5: 50-60 yr (n=15)...
August 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
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