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Semen Analysis

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https://www.readbyqxmd.com/read/28102433/advanced-forensic-validation-for-human-spermatozoa-identification-using-sperm-hy-liter%C3%A2-express-with-quantitative-image-analysis
#1
Ayari Takamura, Ken Watanabe, Tomoko Akutsu
Identification of human semen is indispensable for the investigation of sexual assaults. Fluorescence staining methods using commercial kits, such as the series of SPERM HY-LITER™ kits, have been useful to detect human sperm via strong fluorescence. These kits have been examined from various forensic aspects. However, because of a lack of evaluation methods, these studies did not provide objective, or quantitative, descriptions of the results nor clear criteria for the decisions reached. In addition, the variety of validations was considerably limited...
January 19, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28099575/evaluation-of-sperm-quality-snakes-erythrolamprus-poecilogyrus-sublineatus-cope-1860-serpentes-dipsadidae
#2
A C Silva, A S Varela, T F Cardoso, E F Silva, D Loebmann, C D Corcini
Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution...
January 12, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28090458/efficient-isolation-of-sperm-with-high-dna-integrity-and-stable-chromatin-packaging-by-a-combination-of-density-gradient-centrifugation-and-magnetic-activated-cell-sorting
#3
Hee-Jun Chi, Su-Jin Kwak, Seok-Gi Kim, Youn-Young Kim, Ji-Young Park, Chang-Seok Yoo, Il-Hae Park, Hong-Gil Sun, Jae-Won Kim, Kyeong-Ho Lee
OBJECTIVE: This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS: Semen analysis and a sperm DNA fragmentation assay were performed to assess the correlations between semen parameters and the DFI in 458 semen samples...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28088929/morphologic-analysis-of-sperm-from-two-neotropical-primate-species-comparisons-between-the-squirrel-monkeys-saimiri-collinsi-and-saimiri-vanzolinii
#4
Wlaisa V Sampaio, Karol G Oliveira, Danuza L Leão, Maria C Caldas-Bussiere, Helder L Queiroz, Fernanda P Paim, Regiane R Santos, Sheyla F S Domingues
Sperm morphometry can be applied to identify different animal groups and species and to evaluate sperm quality. Furthermore, knowledge on species-specific differences will help to enhance biological information, as well as to develop efficient reproductive technologies. The aims in the present study were to describe sperm morphometry from the recently characterized species S. collinsi and S. vanzolinii, to verify if the morphometric sperm patterns are similar or different between both species, and to determine if the sperm morphometry is affected by the levels of sperm defects using the S...
January 16, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28088090/degradation-in-forensic-trace-dna-samples-explored-by-massively-parallel-sequencing
#5
Eirik Nataas Hanssen, Robert Lyle, Thore Egeland, Peter Gill
Routine forensic analysis using STRs will fail if the DNA is too degraded. The DNA degradation process in biological stain material is not well understood. In this study we sequenced old semen and blood stains by massively parallel sequencing. The sequence data coverage was used to measure degradation across the genome. The results supported the contention that degradation is uniform across the genome, showing no evidence of regions with increased or decreased resistance towards degradation. Thus the lack of genetic regions robust to degradation removes the possibility of using such regions to further optimize analysis performance for degraded DNA...
January 3, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28087298/the-utility-of-sex-hormone-binding-globulin-in-hypogonadism-and-infertile-males
#6
Joshua Ring, Charles Welliver, Mike Parenteau, Stephen Markwell, Robert E Brannigan, Tobias S Köhler
PURPOSE: We sought to determine what role SHBG played with male infertility patients. METHODS: Retrospective review of 168 males seen in a fertility clinic from 2012-2014, to investigate the accuracy of TT in the biochemical diagnosis of hypogonadism using cBT as the reference value. We used a multivariable analysis to assess SHBG as an independent predictor of infertility. RESULTS: Computations using cBT as a standard in the measurement of definitive biochemical hypogonadism (<156 ng/dL) revealed a sensitivity, specificity, PPV, and NPV respectively of 81%, 83%, 81%, and 82% in diagnosing hypogonadism with TT alone...
January 10, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28078721/use-of-the-fluorescent-dye-tetramethylrhodamine-methyl-ester-perchlorate-for-mitochondrial-membrane-potential-assessment-in-human-spermatozoa
#7
P Uribe, J V Villegas, R Boguen, F Treulen, R Sánchez, P Mallmann, V Isachenko, G Rahimi, E Isachenko
Mitochondrial membrane potential (ΔΨm) is an indicator of sperm quality and its evaluation complements the standard semen analysis. The fluorescent dye JC-1 has been widely used to assess sperm ΔΨm; however, some problems have been detected under certain experimental conditions. Another fluorescent compound, tetramethylrhodamine methyl ester perchlorate (TMRM), has been used in somatic cells and bovine spermatozoa but not in human spermatozoa. TMRM accumulates in hyperpolarised mitochondria and the fluorescence intensity of this compound correlates with ΔΨm...
January 11, 2017: Andrologia
https://www.readbyqxmd.com/read/28078226/clinical-utility-of-sperm-dna-fragmentation-testing-practice-recommendations-based-on-clinical-scenarios
#8
REVIEW
Ashok Agarwal, Ahmad Majzoub, Sandro C Esteves, Edmund Ko, Ranjith Ramasamy, Armand Zini
Sperm DNA fragmentation (SDF) has been generally acknowledged as a valuable tool for male fertility evaluation. While its detrimental implications on sperm function were extensively investigated, little is known about the actual indications for performing SDF analysis. This review delivers practice based recommendations on commonly encountered scenarios in the clinic. An illustrative description of the different SDF measurement techniques is presented. SDF testing is recommended in patients with clinical varicocele and borderline to normal semen parameters as it can better select varicocelectomy candidates...
December 2016: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28073193/analysis-of-myo-inositol-effect-on-spermatozoa-motility-in-hyper-viscous-ejaculates-and-in-patients-with-grades-ii-and-iii-varicocele
#9
Filomena Scarselli, Anna Maria Lobascio, Mario Terribile, Valentina Casciani, Pierfrancesco Greco, Giorgio Franco, Maria Giulia Minasi, Ermanno Greco
The goal of this study is to evaluate MYOInositol effects on spermatozoa motility, in patients' ejaculates with severe varicocele or hyper viscosity. The study included normal viscosity ejaculate from 30 patients affected by varicocele and hyper viscosity ejaculate from 33 patients without any testicular pathologies. All selected samples showed sperm concentration > 2 million/ml and progressive motility < 32%. In both groups, the pellet obtained after centrifugation in buffered medium, was divided in two aliquots, both incubated for 15 minutes at 37°C: one with MYO-Inositol and the other one, as control, only in phosphate buffered saline (PBS)...
December 30, 2016: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/28069178/outcomes-of-anastrozole-in-oligozoospermic-hypoandrogenic-subfertile-men
#10
Ohad Shoshany, Nikita Abhyankar, Naem Mufarreh, Garvey Daniel, Craig Niederberger
OBJECTIVE: To determine whether the change in sperm parameters in subfertile hypoandrogenic men treated with anastrozole is correlated to the magnitude of increase in testosterone (T) to estrogen ratio in men responding to treatment. DESIGN: Retrospective study. SETTING: Male fertility clinic. PATIENT(S): The study group consisted of 86 subfertile hypoandrogenic men with low T/estradiol (E2) ratio (n = 78) or a prior aversive reaction to clomiphene citrate (n = 8)...
January 6, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28063626/effect-of-chamber-characteristics-loading-and-analysis-time-on-motility-and-kinetic-variables-analysed-with-the-casa-mot-system-in-goat-sperm
#11
R Del Gallego, S Sadeghi, E Blasco, C Soler, J L Yániz, M A Silvestre
Several factors unrelated to the semen samples could be influencing in the sperm motility analysis. The aim of the present research was to study the effect of four chambers with different characteristics, namely; slide-coverslip, Spermtrack, ISAS D4C10, and ISAS D4C20 on the sperm motility. The filling procedure (drop or capillarity) and analysis time (0, 120 and 240s), depth of chamber (10 or 20μm) and field on motility variables were analysed by use of the CASA-mot system in goat sperm. Use of the drop-filling chambers resulted in greater values than capillarity-filling chambers for all sperm motility and kinetic variables, except for LIN (64...
December 26, 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/28060836/diagnosis-of-partial-retrograde-ejaculation-in-non-azoospermic-infertile-men-with-low-semen-volume
#12
Roger Mieusset, Marie Walschaerts, François Isus, Thierry Almont, Myriam Daudin, Safouane M Hamdi
In non-azoospermic patients with low semen volume (LSV), looking for partial retrograde ejaculation (PRE) by searching sperm in the postejaculatory urine (PEU) is required. The use of a retro-ejaculatory index (R-ratio) was suggested to define PRE, but none of the studies indicated a specific threshold above which PRE must be considered. Our objective was to propose a threshold value for the R-ratio as indicative of PRE in patients with LSV selected to be devoid of any known causes or risk factors for retrograde ejaculation or LSV...
2017: PloS One
https://www.readbyqxmd.com/read/28058714/a-phytotherapic-approach-to-reduce-sperm-dna-fragmentation-in-patients-with-male-infertility
#13
Marco Capece, Giuseppe Romeo, Antonio Ruffo, Leo Romis, Salvatore Mordente, Giovanni Di Lauro
INTRODUCTION: Infertility affects 50 to 80 million (between 8 and 12% of couples). Male factor is a cause of infertility in almost half of the cases, mainly due to oligoasthenoteratozoospermia. DNA fragmentation is now considered an important factor in the aetiology of male infertility. We studied the effects on semen analysis and on DNA fragmentation of in vivo admnistration of Myo-Inositol and Tribulus Terrestris plus Alga Ecklonia plus Biovis (Tradafertil; Tradapharma Sagl, Swizerland) in men with previously diagnosed male infertility...
December 28, 2016: Urologia
https://www.readbyqxmd.com/read/28046165/proteomic-analysis-of-seminal-plasma-and-sperm-cells-and-their-associations-with-semen-freezability-in-guzerat-bulls
#14
J P A Rego, J M Martins, C A Wolf, M van Tilburg, F Moreno, A C Monteiro-Moreira, R A Moreira, D O Santos, A A Moura
The objective of the present study was to describe the relationship of seminal plasma and total sperm cell proteins with the semen freezability parameters of Guzerat bulls. Thirteen bulls were subjected to breeding soundness evaluation. Semen samples were collected, cryopreserved, and then post-thawing sperm kinetics were assessed, where high ( = 7) and low ( = 6) freezability groups were defined. Seminal plasma and total sperm proteins from the 2 groups were separated by 2-dimensional SDS-PAGE, and spots were identified by mass spectrometry...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28043372/relationships-among-stallion-fertility-and-semen-traits-using-estimated-breeding-values-of-german-warmblood-stallions
#15
Maren Gottschalk, Harald Sieme, Gunilla Martinsson, Ottmar Distl
A high quality of stallion semen is of particular importance for maximum reproductive efficiency. In the present study, we estimated the relationships among estimated breeding values (EBVs) of semen traits and EBVs for the paternal component of the pregnancy rate per estrus cycle (EBV-PAT) for 100 German Warmblood stallions using correlation and general linear model analyses. The most highly correlated sperm quality trait was total number of progressively motile sperm (r = 0.36). EBV-PAT was considered in three classes with stallions 1 SD below (<80), around (80-120), and above (>120) the population mean of 100...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043354/effect-of-sex-sorting-and-cryopreservation-on-the-post-thaw-sperm-quality-of-iberian-red-deer-spermatozoa
#16
L Anel-López, O García-Álvarez, I Parrilla, D Del Olmo, A Maroto-Morales, M R Fernandez-Santos, J A Ortiz, A J Soler, E M Martínez, J M Vazquez, J J Garde
This study investigated the effect of sex-sorting and cryopreservation on post-thaw characteristics and fertility of red deer (Cervus elaphus) sperm for the first time. Semen was collected by electroejaculation from 10 mature stags during the breeding season, and each ejaculate split into four experimental groups: Bulk sorted spermatozoa, sorted but not sexed (BSS); sorted high purity X-spermatozoa (XSS); sorted high purity Y-spermatozoa (YSS); and, control non-sorted spermatozoa (NS). Following, all samples were frozen over liquid nitrogen...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043348/the-value-of-trans-scrotal-ultrasonography-at-bull-breeding-soundness-evaluation-bbse-the-relationship-between-testicular-parenchymal-pixel-intensity-and-semen-quality
#17
Martin Tomlinson, Amy Jennings, Alastair Macrae, Isabelle Truyers
Bull breeding soundness evaluation (BBSE) is commonly undertaken to identify bulls that are potentially unfit for use as breeding sires. Various studies worldwide have found that approximately 20% of the bulls fail their routine prebreeding BBSE and are therefore considered subfertile. Multiple articles describe the use of testicular ultrasound as a noninvasive aid in the identification of specific testicular and epididymal lesions. Two previous studies have hypothesized a correlation between ultrasonographic testicular parenchymal pixel intensity (PI) and semen quality; however to date, no published studies have specifically examined this link...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043341/effect-of-seminal-plasma-removal-before-cryopreservation-of-bovine-semen-obtained-by-electroejaculation-on-semen-quality-and-in%C3%A2-vitro-fertility
#18
Suzane Peres Campanholi, Fabio Morato Monteiro, Erika Aline Ribeiro Dias, Maria Eugênia Zerlotti Mercadante, Claudia Cristina Paro de Paz, José Antonio Dell'Aqua Junior, Frederico Ozanam Papa, Camila de Paula Freitas Dell'Aqua, Roberta Vantini, Joaquim Mansano Garcia
Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28042049/a-novel-approach-of-microscopic-subinguinal-varicocelectomy-with-a-pulling-strategy
#19
Wu Kerong, Yan Guang, Yin Weiqi, Chen Xueqin, Wang Guoyao, Cheng Yue
OBJECTIVES: To investigate the safety and efficacy of a novel approach of microscopic subinguinal varicocelectomy with a "pulling" strategy. METHODS: Fifty-two male patients with varicocele were enrolled in this study. Twenty-six patients underwent conventional microscopic subinguinal varicocelectomy, whereas the remaining 26 patients accepted our novel approach of treatment. In this method, with a subinguinal incision, we dissociated the spermatic cord from the external ring...
December 29, 2016: Urology
https://www.readbyqxmd.com/read/28041731/analysis-of-bull-bos-taurus-seminal-vesicle-fluid-proteome-in-relation-to-seminal-plasma-proteome
#20
B Westfalewicz, M A Dietrich, A Mostek, A Partyka, W Bielas, W Niżański, A Ciereszko
The existing knowledge on the bull seminal vesicle proteome, a major seminal plasma constituent, and its relationship with seminal plasma is limited. This knowledge is prerequisite for a better understanding of seminal plasma variability, which is linked to semen quality. The objective of this study was to characterize the proteomes of seminal vesicle fluid and seminal plasma and to compare them to better understand the origin of seminal plasma proteins. We collected ejaculates and seminal vesicle fluid postmortem from 6 mature Holstein Friesian bulls...
December 29, 2016: Journal of Dairy Science
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