Read by QxMD icon Read

Semen Analysis

Chetna Gangwar, Atul Saxena, Akhil Patel, S P Singh, Sarvajeet Yadav, Ravindra Kumar, Vijay Singh
The experiment was conducted to study cryopreservation induced sperm cryoinjuries and the protective effect of reduced Glutathione supplementation in Murrah bull semen. A total of 20 semen ejaculates were split into two parts after initial examination and were extended in glycerolated egg yolk TRIS diluter (Control group) and glycerolated egg yolk TRIS diluter + 0.5 mM reduced Glutathione (Treatment Group). The diluted semen samples were loaded into 0.25 ml French mini straw and sealing of straws were done...
March 9, 2018: Animal Reproduction Science
Mathew J Tomlinson, Asad Naeem
CASA has been used in reproductive medicine and pathology laboratories for over 25 years, yet the 'fertility industry' generally remains sceptical and has avoided automation, despite clear weaknesses in manual semen analysis. Early implementers had difficulty in validating CASA-Mot instruments against recommended manual methods (haemocytometer) due to the interference of seminal debris and non-sperm cells, which also affects the accuracy of grading motility. Both the inability to provide accurate sperm counts and a lack of consensus as to the value of sperm kinematic parameters appear to have continued to have a negative effect on CASA-Mot's reputation...
March 21, 2018: Reproduction, Fertility, and Development
Yanan Du, Yan Wang, Liqing Du, Chang Xu, Kaihua Ji, Jinhan Wang, Qiang Liu
In this data article, 146 villagers (exposed group) were randomly selected from the workers who involved in the e-wastes recycling directly as a daily job in Tianjin. Control group, including 121 villagers, came from another town without e-waste disposal sites. Chromosomal aberrations (CA) and cytokinesis blocking micronucleus (CBMN) were performed to detect the cytogenetic effect for each subject. DNA damage was detected using comet assay; the DNA percentage in the comet tail (TDNA%), tail moment (TM), and Olive tail moment (OTM) were recorded to describe DNA damage to lymphocytes and spermatozoa...
April 2018: Data in Brief
Zufferey Fanny, Rahban Rita, Garcia Arnaud, Gagnebin Yoric, Boccard Julien, Tonoli David, Jeanneret Fabienne, Stettler Eric, Senn Alfred, Nef Serge, Rudaz Serge, F Rossier Michel
Steroids play an important role in sperm production and quality. These hormones have been extensively studied in blood, but poorly investigated in semen. The purpose of our study was to evaluate the relationship between sperm quality and steroid profiles in blood and semen in a small cohort of young Swiss men. Another objective was to determine whether the presence of xenobiotics or drugs could influence these profiles. Semen analysis was performed according to WHO guidelines, and steroid profiles in blood serum and seminal plasma were determined by two complementary approaches: a targeted investigation involving the quantification of a limited number of relevant steroids for testing putative correlations with sperm parameters and a global "steroidomic" analysis highlighting their complex metabolic relationship...
March 16, 2018: Clinical Biochemistry
Anita Kuriya, Chioma Agbo, Michael H Dahan
OBJECTIVE: To evaluate the relationship of one or a combination of semen analysis parameter results on insemination outcomes. MATERIAL AND METHODS: A retrospective analysis was performed to evaluate the effect on pregnancy rates in relation to one or more abnormal semen analysis parameters based on the 2010 World Health Organization semen analysis guidelines. RESULTS: Nine hundred and eighty one couples underwent 2231 intrauterine insemination cycles at the Stanford Fertility and Reproductive Medicine Center...
March 19, 2018: Journal of the Turkish German Gynecological Association
Ross E Anderson, Heidi A Hanson, Diana Thai, Chong Zhang, Angela P Presson, Kenneth I Aston, Douglas T Carrell, Ken R Smith, James M Hotaling
PURPOSE: To study the role of individual semen parameters on the offspring birth weight and body mass index (BMI) from a population of men evaluated in an assisted reproduction technology (ART) clinic compared to fertile controls. METHODS: We performed a retrospective study using a cohort with fertile, age-matched controls of men evaluated with semen analysis at the University of Utah Andrology Clinic from 1996 to 2011 and Intermountain Healthcare from 2002 to 2011...
March 17, 2018: Journal of Assisted Reproduction and Genetics
Kristel Ehala-Aleksejev, Margus Punab
This study aimed to determine the effect of metabolic syndrome (MS) on the reproductive function in fertile (FM) and male partners of infertile couples (MPIC). We performed a cross-sectional study formatting two study groups: partners of pregnant women (n = 238; mean age 32.0) as FM and male partners of infertile couples (n = 2642; mean age 32.6) as MPIC. A standard semen analysis was performed and clinical, laboratory and lifestyle data were analysed. The adapted NCEP-ATPIII criteria were used to define MS...
2018: PloS One
Jin-Chun Lu, Jun Jing, Li Chen, Yi-Feng Ge, Rui-Xiang Feng, Yuan-Jiao Liang, Bing Yao
BACKGROUND: Many factors may lead to sperm DNA damage. However, it is little known that the correlations of sperm DNA damage with obesity-associated markers, and reproductive hormones and lipids levels in serum and seminal plasma. METHODS: In our prospective study, a total of 1 010 subfertile men, aged from 18 to 50 years old, were enrolled from August 2012 through June 2015. Their obesity-associated markers, semen parameters, sperm acrosomal enzyme activity, seminal plasma biochemical markers, and reproductive hormones and lipids levels in serum and seminal plasma were detected...
March 14, 2018: Reproductive Biology and Endocrinology: RB&E
A Mostek, M Slowinska, S Judycka, H Karol, A Ciereszko, M A Dietrich
During semen cryopreservation, spermatozoa are exposed to physical and chemical stressors that result in their functional and structural damage. Growing evidence suggests that most cryoinjuries result from oxidative stress accompanying sperm cryopreservation. Elevated amounts of reactive oxygen species (ROS) generated during cryopreservation can react with sperm macromolecules, including proteins. The goal of this study was to investigate the oxidative modifications (measured as carbonylation level changes) of carp spermatozoa proteins triggered by the cryopreservation process...
March 9, 2018: Journal of Animal Science
Luna Samanta, Ashok Agarwal, Nirlipta Swain, Rakesh Sharma, Banu Gopalan, Sandro C Esteves, Damayanthi Durairajanayagam, Edmund Sabanegh
PURPOSE: Varicocele may disrupt testicular microcirculation and induce hypoxia-ischemia related degenerative changes in testicular cells and spermatozoa. Superoxide production at low oxygen concentrations exacerbates oxidative stress in men with varicocele. Therefore, the present study was designed to study the role of mitochondrial redox regulation and its possible involvement in sperm dysfunction in varicocele-associated infertility. MATERIALS AND METHODS: Differentially expressed mitochondrial proteins were identified in 50 infertile men with varicocele and ten fertile controls by secondary LC-MS/MS data-driven in silico analysis...
March 9, 2018: Journal of Urology
Frank Tüttelmann, Christian Ruckert, Albrecht Röpke
Infertility is a common condition estimated to affect 10-15% of couples. The clinical causes are attributed in equal parts to the male and female partners. Diagnosing male infertility mostly relies on semen (and hormone) analysis, which results in classification into the two major phenotypes of oligo- and azoospermia. The clinical routine analyses have not changed over the last 20 years and comprise screening for chromosomal aberrations and Y‑chromosomal azoospermia factor deletions. These tests establish a causal genetic diagnosis in about 4% of unselected men in infertile couples and 20% of azoospermic men...
2018: Medizinische Genetik: Mitteilungsblatt des Berufsverbandes Medizinische Genetik E.V
Patricia P Albani, Rachel Fleming
In forensic investigations, the identification of the cellular or body fluid source of biological evidence can provide crucial probative information for the court. Messenger RNA (mRNA) profiling has become a valuable tool for body fluid and cell type identification due to its high sensitivity and compatibility with DNA analysis. However, using a single marker to determine the somatic origin of a sample can lead to misinterpretation as a result of cross-reactions. While false positives may be avoided through the simultaneous detection of multiple markers per body fluid, this approach is currently limited by the small number of known differentially expressed mRNAs...
March 2018: Science & Justice: Journal of the Forensic Science Society
Rulin Dai, Yuan Pan, Yan Fu, Qian Liu, Weifeng Han, Ruizhi Liu
OBJECTIVE: This study aimed to investigate the roles of male genetic factors, including Y chromosome microdeletions and chromosomal heteromorphism, in recurrent pregnancy loss (RPL) in Northeast China. STUDY DESIGN: We evaluated 1072 male patients from Northeast China whose wives had a history of two or more consecutive miscarriages. We also selected 971 infertile and 200 fertile men as control groups. Semen analysis was carried out by computer-assisted sperm analysis...
March 1, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Ivona Žura Žaja, Silvijo Vince, Suzana Milinković-Tur, Nina Poljičak Milas, Marko Samardžija, Hrvoje Valpotić, Velimir Berta, Marinko Vilić, Kristina Rakić
The aim of this study was to establish subpopulations of spermatozoa in bucks using the principal component (PC) and cluster analysis according to morphometric head and tail variables, and to determine differences in proportions of subpopulations between exogenous melatonin-treated and control bucks. The bucks (n = 12) were assigned to two groups comprising six bucks each. By the end of March, four melatonin implants were inserted in the bucks in the experimental group. Semen was collected weekly using an artificial vagina from March to May (the non-breeding season)...
March 5, 2018: Animal Reproduction Science
Vera Haapala, Tarja Pohjanvirta, Nella Vähänikkilä, Jani Halkilahti, Henri Simonen, Sinikka Pelkonen, Timo Soveri, Heli Simojoki, Tiina Autio
Mycoplasma bovis infections are responsible for substantial economic losses in the cattle industry, have significant welfare effects and increase antibiotic use. The pathogen is often introduced into naive herds through healthy carrier animals. In countries with a low prevalence of M. bovis, transmission from less common sources can be better explored as the pathogen has limited circulation compared to high prevalence populations. In this study, we describe how M. bovis was introduced into two closed and adequately biosecure dairy herds through the use of contaminated semen during artificial insemination (AI), leading to mastitis outbreak in both herds...
March 2018: Veterinary Microbiology
J L Yániz, M A Silvestre, P Santolaria, C Soler
Sperm motility is one of the most widely used parameters of sperm quality. Computer-aided sperm motility analysis (CASA-Mot) systems were developed to reduce the subjectivity of sperm motility assessment, and have had broad scientific and practical acceptance. In this review, the sources of variation and current applications of this technology and its relationships with other sperm quality tests are described in detail. Despite remarkable advances in the technique, there is still great need for standardisation in many species, and the numerous factors that affect the results make it difficult to provide universally accepted criteria for classifying semen samples based on sperm motility characteristics...
March 8, 2018: Reproduction, Fertility, and Development
I David, P Kohnke, J Fehrenbach, A R Lopes Simoes, E Debreuve, X Descombes, F Plouraboue, P Degond, X Druart
In sheep, wave motion in semen is currently used by AI centres to select ejaculates for insemination. Despite its low cost, convenience and established ability to predict fertility, the subjectivity of this assessment is a limiting factor for its applicability. The aims of the present study were to establish an objective method for the analysis of wave motion and to assess the associations of objective parameters with fertility after cervical insemination. Collective sperm motion in undiluted semen was observed by phase contrast microscopy at low magnification in a 100-µm deep glass chamber...
March 8, 2018: Reproduction, Fertility, and Development
Katerina Chatzimeletiou, Nikiforos Galanis, Alexandros Karagiannidis, Antonia Sioga, George Pados, Dimitrios Goulis, Antonis Kalpatsanidis, Basil C Tarlatzis
Ankylosing spondylitis affects 0.1%-0.5% of the adult population. The aim was to investigate the possible effects of both the disease and its treatment on semen quality by performing a highly detailed analysis in a man with ankylosing spondylitis, presenting for infertility. Sperm characteristics were evaluated by light microscopy, morphology by electron microscopy (transmission electron microscopy), DNA fragmentation by terminal deoxynucleotidyl transferase dUTP nick end labeling using fluorescence microscopy and chromosomal abnormalities by fluorescence in situ hybridisation using probes for chromosomes 13,15,16,18,21,22,X and Y...
2018: SAGE Open Medical Case Reports
Molinaro Francesco, Cobellis Giovanni, Cerchia Elisa, Ferrara Francesco, Tallarico Rosella, Sica Marina, Noviello Carmine, Martino Ascanio, Angotti Rossella, Messina Mario
Background: The varicocele is the most commonly diagnosed pre-pubertal andrological condition with an incidence of 10%-15% between adolescents. The aim of this study was to evaluate the efficacy of retroperitoneoscopic varicocelectomy (RV) in two Italian centres with particular attention to post-operative testicular volume and semen analysis after 18 years of life. Materials and Methods: From 1999 to 2010, 286 adolescents underwent retroperitoneoscopic left varicocelectomy...
April 2017: African Journal of Paediatric Surgery: AJPS
Nischay Mishra, Adrian Caciula, Adam Price, Riddhi Thakkar, James Ng, Lokendra V Chauhan, Komal Jain, Xiaoyu Che, Diego A Espinosa, Magelda Montoya Cruz, Angel Balmaseda, Eric H Sullivan, Jigar J Patel, Richard G Jarman, Jennifer L Rakeman, Christina T Egan, Chantal B E M Reusken, Marion P G Koopmans, Eva Harris, Rafal Tokarz, Thomas Briese, W Ian Lipkin
Zika virus (ZIKV) is implicated in fetal stillbirth, microcephaly, intracranial calcifications, and ocular anomalies following vertical transmission from infected mothers. In adults, infection may trigger autoimmune inflammatory polyneuropathy. Transmission most commonly follows the bite of infected Aedes mosquitoes but may also occur through sexual intercourse or receipt of blood products. Definitive diagnosis through detection of viral RNA is possible in serum or plasma within 10 days of disease onset, in whole blood within 3 weeks of onset, and in semen for up to 3 months...
March 6, 2018: MBio
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"