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Semen Analysis

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https://www.readbyqxmd.com/read/28213237/revealing-the-location-of-semen-vaginal-fluid-and-urine-in-stained-evidence-through-near-infrared-chemical-imaging
#1
Félix Zapata, Fernando E Ortega-Ojeda, Carmen García-Ruiz
Crime scene investigation (CSI) requires the ultimate available technology for a rapid, non-destructive, and accurate detection of a wide variety of evidence including invisible stains of bodily fluids. Particularly crucial is the discrimination of semen in stained evidence from sexual abuse cases. This is because those evidence have high odds of containing the DNA from the aggressor. To this aim, we demonstrated the potential of near infrared hyperspectral imaging (NIR-HSI) to make visible stains of semen, vaginal fluid, and urine on fabrics, which lays the bases to face the challenging visualization and discrimination of semen within bodily fluids mixtures...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28187743/the-influence-of-macro-and-microelements-in-seminal-plasma-on-diluted-boar-sperm-quality
#2
Maja Zakošek Pipan, Janko Mrkun, Breda Jakovac Strajn, Katarina Pavšič Vrtač, Janko Kos, Anja Pišlar, Petra Zrimšek
BACKGROUND: Growing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality parameters after 3 days of liquid storage was assessed. Twenty ejaculates from eight boars were collected, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability, mitochondrial membrane potential and DNA fragmentation were determined on the day of collection (day 0) and day 3 (72 h) of storage at 15-17 °C...
February 10, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28187518/male-caffeine-and-alcohol-intake-in-relation-to-semen-parameters-and-in%C3%A2-vitro-fertilization-outcomes-among-fertility-patients
#3
A E Karmon, T L Toth, Y-H Chiu, A J Gaskins, C Tanrikut, D L Wright, R Hauser, J E Chavarro
Much of the literature on the impact of male caffeine and alcohol intake on reproductive outcomes has utilized semen quality as a proxy for male fertility, although semen parameters have a limited predictive value for spontaneous pregnancy. The objective of this study was to investigate whether male caffeine and alcohol intakes are associated with semen parameters and assisted reproductive technology outcome. The Environment and Reproductive Health Study, an ongoing prospective cohort study, enrolls subfertile couples presenting for treatment at an academic fertility center (2007-2012)...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28187507/diagnostic-genetic-screening-for-assisted-reproductive-technologies-patients-with-macrozoospermia
#4
V Carmignac, J-M Dupont, R C Fierro, J Barberet, C Bruno, N Lieury, E Dulioust, J Auger, P Fauque
Macrozoospermia is characterized by a high proportion of abnormal spermatozoa with enlarged heads. So far, it has been associated with mutations only in the Aurora Kinase C gene (AURKC) in some cases. Although many publications have reported failure to conceive in couples with macrozoospermia, a few others have described successful pregnancies, thus raising questions as to whether ICSI and AURKC genetic screening should be recommended in all patients with macrozoospermia. First, we report on two monozygotic twins presenting macrozoospermia for whom the genetic status was explored (Aurora Kinase C sequencing) and whole semen and gradient-selected spermatozoa were analyzed, using Fluorescent In Situ Hybridization (FISH), Electron Microscopy and flow cytometry...
February 10, 2017: Andrology
https://www.readbyqxmd.com/read/28185841/varicocele-embolization-with-glue-and-coils-a-single-center-experience
#5
C Bilreiro, P Donato, J F Costa, A Agostinho, V Carvalheiro, F Caseiro-Alves
PURPOSE: The purpose of this study was to compare metallic coils and glue (n-butyl-2 cyanoacrylate) for varicocele embolization, regarding immediate technical and clinical success, procedure time, complications and recurrence rates. MATERIALS AND METHODS: A retrospective analysis of a consecutive series of varicocele embolization procedures performed between July 2012 and July 2015 was undertaken. A total of 129 procedures were performed, 26 using glue (20.2%; 26 men with a mean age of 32...
February 6, 2017: Diagnostic and Interventional Imaging
https://www.readbyqxmd.com/read/28166982/dietary-polyunsaturated-fatty-acid-supplementation-of-young-post-pubertal-dairy-bulls-alters-the-fatty-acid-composition-of-seminal-plasma-and-spermatozoa-but-has-no-effect-on-semen-volume-or-sperm-quality
#6
C J Byrne, S Fair, A M English, S A Holden, J R Dick, P Lonergan, D A Kenny
The aim of this study was to examine the effects of dietary supplementation with rumen protected n-6 or n-3 polyunsaturated fatty acids (PUFA) on the quantity and quality of semen from young post-pubertal dairy bulls. Pubertal Holstein-Friesian (n = 43) and Jersey (n = 7) bulls with a mean ± s.e.m. age and bodyweight of 420.1 ± 5.86 days and 382 ± 8.94 kg, respectively, were blocked on breed, weight, age and semen quality (based on the outcomes of two pre-trial ejaculates) and randomly assigned to one of three treatments: (i) a non-supplemented control (CTL, n = 15), (ii) rumen-protected safflower (SO, n = 15), (iii) rumen-protected n-3 PUFA-enriched fish oil (FO, n = 20)...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166964/toxoplasma-gondii-transmission-by-artificial-insemination-in-sheep-with-experimentally-contaminated-frozen-semen
#7
Angélica Consalter, Andressa F Silva, Edwards Frazão-Teixeira, Luis F Matos, Francisco C R de Oliveira, Juliana S Leite, Franciele B F Silva, Ana M R Ferreira
Toxoplasma gondii is a parasite considered one of the major causes of reproductive problems in sheep. Furthermore, the presence of the agent in ram semen urges the possibility of sexual transmission in this species. The aim of this study was to evaluate if ram's frozen semen spiked with T. gondii tachyzoites would be able to cause infection in sheep by laparoscopic artificial insemination (AI). Nine ewes tested seronegative to anti-T. gondii antibodies by the modified agglutination test (MAT) were superovulated and inseminated to collect embryos...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166956/impact-of-induced-levels-of-specific-free-radicals-and-malondialdehyde-on-chicken-semen-quality-and-fertility
#8
Bruno R Rui, Fábio Y Shibuya, Allison J T Kawaoku, João D A Losano, Daniel S R Angrimani, Andressa Dalmazzo, Marcilio Nichi, Ricardo J G Pereira
Over the past decades, scientists endeavored to comprehend oxidative stress in poultry spermatozoa and its relationship with fertilizing ability, lipid peroxidation (LPO), free-radical scavenging systems, and antioxidant therapy. Although considerable progress has been made, further improvement is needed in understanding how specific reactive oxygen species (ROS) and malondialdehyde (MDA, a toxic byproduct of LPO) disrupt organelles in avian spermatozoon. Hence, this study examined functional changes in chicken spermatozoa after incubation with different ROS, and their implications for the fertility...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28163813/hematospermia-in-a-returned-traveler
#9
Raynell Lang, Jessica Minion, Alexander Wong
Hematospermia is a common complaint among patients seen in outpatient urology clinics. The differential diagnosis is broad and includes inflammatory, infectious, neoplastic, structural, systemic, and traumatic causes. The most common infectious causes are uropathogens and sexually transmitted infections. However, with increasing global travel, physicians must maintain a high clinical suspicion for pathogens not endemic to their region, including Echinococcus, Mycobacterium tuberculosis, and Schistosoma.1 We present a case of hematospermia in a traveler returning from Eastern Africa with exposure to Lake Malawi...
January 2017: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/28161937/gonadal-and-sexual-dysfunction-in-childhood-cancer-survivors
#10
Ju Young Yoon, Hyeon Jin Park, Hee Young Ju, Jong Hyung Yoon, Jin Soo Chung, Sang Hyun Hwang, Dong Ock Lee, Hye Young Shim, Byung-Kiu Park
Purpose: Few studies have addressed gonadal and sexual dysfunctions in childhood cancer survivors. We evaluated the prevalence rates and risk factors for gonadal failure among adolescent/young adult childhood cancer survivors and their sexual function. Materials and Methods: Subjects were childhood cancer survivors aged 15-29 years who had completed therapy more than two years ago. Demographic and medical characteristics were obtained from the patients' medical records...
January 25, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/28161861/aryl-hydrocarbon-receptor-ahr-rs2066853-gene-polymorphism-association-with-infertile-oligoasthenoteratozoospermic-men-and-seminal-oxidative-stress
#11
Taymour Mostafa, Hanan Fouad, Nashaat Nabil, Laila Rashed, Dina Sabry, Khadiga Abougabal, Bolis S Gendy
This study aimed to assess the association between aryl hydrocarbon receptor (AhR) rs2066853 gene polymorphism with infertile oligoasthenoteratozoospermic (OAT) men and seminal oxidative stress (OS). A total of 170 Egyptian men were allocated according to their semen analysis into fertile normozoospermic controls (n = 50) and infertile OAT men (n = 120). They were subjected to history taking, clinical examination, semen analysis, estimation of seminal glutathione peroxidase (GPx), and malondialdehyde (MDA)...
February 4, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28159940/the-impact-of-bmi-on-sperm-parameters-and-the-metabolite-changes-of-seminal-plasma-concomitantly
#12
Dan Guo, Wei Wu, Qiuqin Tang, Shanlei Qiao, Yiqiu Chen, Minjian Chen, Mengying Teng, Chuncheng Lu, Hongjuan Ding, Yankai Xia, Lingqing Hu, Daozhen Chen, Jiahao Sha, Xinru Wang
The development of male infertility increased rapidly worldwide, which coinciding with the epidemic of obesity. However, the impact of weight abnormalities on sperm quality is still contestable. To assess the correlation between BMI and sperm parameters, we searched relevant articles in PubMed, Embase, Web of science, and Wanfang database published until June 2015 without language restriction. Otherwise, we also recruited some participants who attended fertility clinic as well as some general populations in this report...
January 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28158219/tissue-specific-features-of-the-x-chromosome-and-nucleolus-spatial-dynamics-in-a-malaria-mosquito-anopheles-atroparvus
#13
Semen M Bondarenko, Gleb N Artemov, Igor V Sharakhov, Vladimir N Stegniy
Spatial organization of chromosome territories is important for maintenance of genomic stability and regulation of gene expression. Recent studies have shown tissue-specific features of chromosome attachments to the nuclear envelope in various organisms including malaria mosquitoes. However, other spatial characteristics of nucleus organization, like volume and shape of chromosome territories, have not been studied in Anopheles. We conducted a thorough analysis of tissue-specific features of the X chromosome and nucleolus volume and shape in follicular epithelium and nurse cells of the Anopheles atroparvus ovaries using a modern open-source software...
2017: PloS One
https://www.readbyqxmd.com/read/28148467/comparison-of-fertility-of-liquid-or-frozen-semen-when-varying-the-interval-from-cidr-removal-to-insemination
#14
Brittany N Richardson, Erin L Larimore, Julie A Walker, Matthew D Utt, J Mel DeJarnette, George A Perry
Cryopreservation allows for long-term storage of semen; however, it leads to damage of sperm that may result in complete loss of viability or changes that possibly decrease sperm functionality. Liquid semen is not exposed to these stressors and may result in a longer lifespan in the female reproductive tract, thus increasing the range in timing of insemination without affecting fertility. The objective of this study was to compare fertility of liquid and frozen semen when varying the interval from CIDR removal to AI using the 7-day CO-Synch+CIDR protocol for synchronization of time of estrus...
January 24, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28138186/relationship-of-seminal-free-l-carnitine-with-functional-spermatozoal-characteristics-results-from-an-observational-study-conducted-in-a-tertiary-care-hospital-of-karachi-pakistan
#15
Syed Danish Haseen Ahmed, Shahid Ahsan, Tehseen Iqbal, Syed Intesar Ahmed Burney
OBJECTIVE: To investigate the relationship of seminal free L-carnitine with functional spermatozoal characteristics. METHODS: This observational study was conducted at the Jinnah Postgraduate Medical Centre, Karachi, from August 2009 to June 2013, and comprised fertile and infertile subjects. Semen analysis was performed and reported for its volume, sperm count, motility and morphology according to the World Health Organisation's guidelines. Seminal free L-carnitine was estimated by high-performance liquid chromatography...
February 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28133770/in-vitro-antioxidant-effect-of-curcumin-on-human-sperm-quality-in-leucocytospermia
#16
L Zhang, R Y Diao, Y G Duan, T H Yi, Z M Cai
Decreased sperm quality was caused by oxidative stress in semen from patients with leucocytospermia. Curcumin is a traditional Chinese herbal monomer extracted from Zingiberaceae turmeric and zedoary turmeric and has antioxidative and anti-inflammatory effects. This study aimed to investigate the effects and specific molecular mechanisms of curcumin on sperm quality in patients diagnosed with leucocytospermia. Forty cases of semen samples were collected from patients with leucocytospermia and 35 cases from normal fertile male...
January 30, 2017: Andrologia
https://www.readbyqxmd.com/read/28131504/childhood-cancer-risk-in-the-siblings-and-cousins-of-men-with-poor-semen-quality
#17
Ross E Anderson, Heidi A Hanson, William T Lowrance, Jeffrey Redshaw, Siam Oottamasathien, Anthony Schaeffer, Erica Johnstone, Kenneth I Aston, Douglas T Carrell, Patrick Cartwright, Ken R Smith, James M Hotaling
PURPOSE: Poor semen quality is associated with reduced somatic health and increased cancer risk. Infertility and cancer are increasingly being linked by epidemiologists and basic scientists. We sought to identify semen parameters associated with an increased childhood cancer risk in the family members of subfertile men. MATERIALS AND METHODS: We performed a retrospective cohort study in men from the SHARE (Subfertility Heath and Assisted Reproduction) study who underwent semen analysis between 1994 and 2011...
January 25, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28130636/molecular-epidemiology-of-pseudorabies-virus-in-yunnan-and-the-sequence-analysis-of-its-gd-gene
#18
Chunlian Song, Libo Gao, Weibing Bai, Xingqin Zha, Gefen Yin, Xianghua Shu
Outbreaks of pseudorabies (PRs) have occurred in Yunnan, China, which caused significant economic loss. To determine the prevalence and origin of PR in Yunnan, especially among vaccinated pigs, overall 791 samples of blood, tissue, semen, and sera were analyzed by serological methods, PCR, and sequence analysis of gD gene. Detection with viral gI antibody or PCR showed that the yearly positive rates of PR virus (PRV) in Yunnan from 2010 to 2014 were 48.15, 21.26, 2.17, 5.22, and 0.35%, respectively, with an average of 15...
January 28, 2017: Virus Genes
https://www.readbyqxmd.com/read/28129552/the-pharmacokinetics-and-tissue-distribution-of-coumaroylspinosin-in-rat-a-novel-flavone-c-glycoside-derived-from-zizyphi-spinosi-semen
#19
Xiaotong Zhao, Junjun Liu, Zhiyou Wen, Yanqing Zhang, Mingxin Yu, Bingcheng Pan, Jun Zeng, Junbo Xie
Zizyphi Spinosi Semen (ZSS) has a long history of sedative-hypnotic use in China. As a novel flavone C-glycoside, coumaroylspinosin is a main flavonoid only found in ZSS. Up to now, its pharmacokinetic information and tissue distribution in vivo are not available yet. With a simple, rapid and sensitive HPLC-MS/MS method, the pharmacokinetics and tissue distribution of coumaroylspinosin were investigated in Sprague-Dawley (SD) rats after its intravenous administration. Puerarin was used as the internal standard (IS)...
January 22, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28128993/environmental-factors-contributed-to-circannual-rhythm-of-semen-quality
#20
Huan Mao, Lei Feng, Wan-Xi Yang
We investigated whether human semen parameters present circannual rhythm or not, and whether environmental factors exert on semen quality. This retrospective study used data of patients mainly from Reproductive Medicine Center and Urology and Andrology Clinic of a general hospital in China. Sperm concentration and motility were measured by computer aided sperm analysis (CASA). Sperm morphology was scored based on the strict criteria (WHO, 2010). The Kruskal-Wallis rank test was used to investigate the relationship between semen parameters and season/month...
January 27, 2017: Chronobiology International
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