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Semen Analysis

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https://www.readbyqxmd.com/read/28444612/wbp2nl-pawp-mrna-and-protein-expression-in-sperm-cells-are-not-related-to-semen-parameters-fertilization-rate-or-reproductive-outcome
#1
T Freour, M Barragan, A Ferrer-Vaquer, A Rodríguez, Rita Vassena
PURPOSE: WBP2NL/PAWP, a protein found in the post-acrosomal region of mammalian spermatozoa, has been proposed as a sperm-borne oocyte-activating factor (SOAF) contributing to Ca(2+) release within the oocyte and subsequent fertilization and embryo development. However, its relevance as either a diagnostic or a prognostic marker of fertilization failure has been questioned in the recent literature. We analyzed WBP2NL/PAWP gene and protein expression level and localization in patients without previous intracytoplasmic sperm injection (ICSI) cycles in order to assess its association with both sperm characteristics and ability to fertilize...
March 21, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28444255/effect-of-cranial-irradiation-on-sperm-concentration-of-adult-survivors-of-childhood-acute-lymphoblastic-leukemia-a-report-from-the-st-jude-lifetime-cohort-study%C3%A2
#2
Daniel M Green, Liang Zhu, Mingjuan Wang, Wassim Chemaitilly, DeoKumar Srivastava, William H Kutteh, Raymond W Ke, Charles A Sklar, Ching-Hon Pui, Larry E Kun, Raul C Ribeiro, Leslie L Robison, Melissa M Hudson
STUDY QUESTION: Does lower dose (<26 Gy) cranial radiation therapy (CRT) used for central nervous system prophylaxis in acute lymphoblastic leukemia (ALL) adversely affect sperm concentration or morphology? SUMMARY ANSWER: CRT doses <26 Gy had no demonstrable adverse effect on sperm concentration or morphology. WHAT IS KNOWN ALREADY: Treatment with alkylating agents produces oligospermia and azoospermia in some patients. No prior study has been large enough to evaluate the independent effects of alkylating agents and lower dose (<26 Gy) CRT on sperm concentration or morphology...
April 21, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28443587/role-of-trace-elements-for-oxidative-status-and-quality-of-human-sperm
#3
Galina Nenkova, Lubomir Petrov, Albena Alexandrova
BACKGROUND: Oxidative stress affects negatively the sperm quality. For maintaining the pro/antioxidative balance, some metal ions (e.g. copper, zinc, iron, selenium), which are co-factors of the antioxidant enzymes, are essential. However, iron and copper could act as prooxidants inducing oxidative damage of spermatozoa. AIMS: This study aims to reveal a possible correlation between the concentrations of some metal ions (iron, copper, zinc, and selenium) in human seminal plasma, oxidative stress, assessed by malondialdehyde (MDA) and total glutathione (tGSH) levels, and semen quality, assessed by the parameters count, motility, and morphology...
April 6, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28441854/-correlation-between-anatomical-factors-of-spermatic-vessels-and-varicocele
#4
L Y Zhao, R H Tian, Y H Huang, H X Chen, P Li, Z Wan, C C Yao, C Yang, E L Zhi, Z Li
Objective: To analyze the correlation between anatomy of spermatic vessels and varicocele, providing reference for the preoperative assessment and treatment of varicocele. Methods: A total of 156 patients who underwent microsurgical left subinguinal varicocelectomy at Shanghai General Hospital between May 2015 and July 2016 were included in this study. The severity of varicocele and number of spermatic vessels detected in operations were recorded. According to the number of internal spermatic arteries (ISAs), the patients were divided into three groups: single-ISA group (55 cases), double-ISAs group (63 cases) and multi-ISAs group (38 cases), to analyze the correlation among spermatic vessels and to compare varicocele grade, the volume of testes, the parameter of semen analysis, serum reproductive hormone, surgery time, and hospital stay among the three groups...
April 25, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28441547/phthalate-exposure-even-below-us-epa-reference-doses-was-associated-with-semen-quality-and-reproductive-hormones-prospective-marhcs-study-in-general-population
#5
Qing Chen, Huan Yang, Niya Zhou, Lei Sun, Huaqiong Bao, Lu Tan, Hongqiang Chen, Xi Ling, Guowei Zhang, Linping Huang, Lianbing Li, Mingfu Ma, Hao Yang, Xiaogang Wang, Peng Zou, Kaige Peng, Taixiu Liu, Xiefei Shi, Dejian Feng, Ziyuan Zhou, Lin Ao, Zhihong Cui, Jia Cao
BACKGROUND: Environment-Protection-Agency Reference Doses (EPA RfDs) for phthalate intakes are based on limited evidence, especially regarding low-dose male-reproductive toxicity. This study investigates the association between phthalate exposure and semen parameters and reproductive hormones in a general population with low phthalate exposure compared to the EPA RfDs. METHODS: The MARHCS (Male-Reproductive-Health-in-Chongqing-College-Students) cohort recruited 796 male students, who experienced a relocation of campuses and shifting environmental exposure...
April 22, 2017: Environment International
https://www.readbyqxmd.com/read/28440264/use-of-testicular-sperm-for-intracytoplasmic-sperm-injection-in-men-with-high-sperm-dna-fragmentation-a-swot-analysis
#6
Sandro C Esteves, Matheus Roque, Nicolás Garrido
Spermatozoa retrieved from the testis of men with high levels of sperm DNA fragmentation (SDF) in the neat semen tend to have better DNA quality. Given the negative impact of SDF on the outcomes of Assisted Reproductive Technology (ART), an increased interest has emerged about the use of testicular sperm for intracytoplasmic sperm injection (Testi-ICSI). In this article, we used a SWOT (strengths, weaknesses, opportunities, and threats) analysis to summarize the advantages and drawbacks of this intervention...
April 18, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28439698/synergistic-effects-of-cigarette-smoking-and-varicocele-on-semen-parameters-in-715-patients
#7
Badereddin Mohamad Al-Ali, Klaus Eredics
OBJECTIVE: To evaluate whether there is a synergistic effect of varicocele and smoking status on semen parameters. METHODS: A retrospective evaluation of 715 men attending an infertility clinic between 2001 and 2009 was carried out. The presence of a varicocele was determined by clinical examination and cigarette smoking habits were documented allowing patients to be divided into 4 groups: non-smokers (n = 369), mild smokers (n = 186, 1-10 cigarettes/day), moderate smokers (n = 129, 10-20 cigarettes/day) and heavy smokers (n = 31, over 20 cigarettes/day)...
April 24, 2017: Wiener Klinische Wochenschrift
https://www.readbyqxmd.com/read/28434653/a-revised-prediction-model-for-natural-conception
#8
Alexandra J Bensdorp, Jan Willem van der Steeg, Pieternel Steures, J Dik F Habbema, Peter G A Hompes, Patrick M M Bossuyt, Fulco van der Veen, Ben W J Mol, Marinus J C Eijkemans
One of the aims in reproductive medicine is to differentiate between couples that have favourable chances of conceiving naturally and those that do not. Since the development of the prediction model of Hunault, characteristics of the subfertile population have changed. The objective of this analysis was to assess whether additional predictors can refine the Hunault model and extend its applicability. Consecutive subfertile couples with unexplained and mild male subfertility presenting in fertility clinics were asked to participate in a prospective cohort study...
March 28, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28431864/toward-an-integrative-and-predictive-sperm-quality-analysis-in-bos-taurus
#9
J L Yániz, C Soler, C Alquézar-Baeta, P Santolaria
There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS(®)3Fun kit, were performed...
March 31, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28431403/association-of-the-vdac3-gene-polymorphism-with-sperm-count-in-han-chinese-population-with-idiopathic-male-infertility
#10
Lianjun Pan, Qingzhen Liu, Jingyun Li, Wei Wu, Xinru Wang, Dan Zhao, Jiehua Ma
Voltage-dependent anion channel (VDAC) is a multifunctional channel protein across the outer mitochondrial membrane of somatic cells and participates in many physiological and pathophysiological processes. Up to now, only a few studies, including our previous studies, showed that VDAC exists in mammalian spermatozoa and is involved in spermatogenesis and sperm functions. There is no report about VDAC genetic variants in germinal tissues or cells. To investigate the possible association between VDAC genetic variants and human sperm quality, we performed semen analysis and variant Genotyping of VDAC3 subtype (rs7004637, rs16891278 and rs6773) of 523 Han-Chinese males with idiopathic infertility respectively by computer assisted semen analysis (CASA) and single nucleotide polymorphism (SNP) Genotyping assay...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28431025/1h-magnetic-resonance-spectroscopy-of-live-human-sperm
#11
S Reynolds, S J Calvert, M N Paley, A A Pacey
STUDY QUESTION: Can 1H Magnetic Resonance Spectroscopy (MRS) be used to obtain information about the molecules and metabolites in live human spermatozoa? SUMMARY ANSWER: Percoll-based density gradient centrifugation (DGC) followed by a further two washing steps, yielded enough sperm with minimal contamination (<0.01%) from seminal fluid to permit effective MRS which detected significant differences (p < 0.05) in the choline/glycerophosphocholine, lipid and lactate regions of the 1H MRS spectrum between sperm in the pellet and those from the 40/80% interface...
April 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28426717/adjusting-cryodiluent-composition-for-improved-post-thaw-quality-of-rabbit-spermatozoa
#12
Sally E Hall, Cameron Negus, Danielle Johinke, Roslyn Bathgate
Improved fertility following artificial insemination with frozen-thawed spermatozoa would offer rabbit producers faster genetic improvement. Previous work investigating cryoprotectants for rabbit spermatozoa have reported inconsistent results. Semen was collected from three rabbit bucks by artificial vagina and frozen using a standard procedure with varied cryodiluent components. Post-thaw analysis encompassed motility, sperm kinematic parameters and acrosome and membrane integrity. Spermatozoa were evaluated at 0, 2 and 4 h after thawing...
2017: PloS One
https://www.readbyqxmd.com/read/28425485/red-light-improves-spermatozoa-motility-and-does-not-induce-oxidative-dna-damage
#13
Daryl Preece, Kay W Chow, Veronica Gomez-Godinez, Kyle Gustafson, Selin Esener, Nicole Ravida, Barbara Durrant, Michael W Berns
The ability to successfully fertilize ova relies upon the swimming ability of spermatozoa. Both in humans and in animals, sperm motility has been used as a metric for the viability of semen samples. Recently, several studies have examined the efficacy of low dosage red light exposure for cellular repair and increasing sperm motility. Of prime importance to the practical application of this technique is the absence of DNA damage caused by radiation exposure. In this study, we examine the effect of 633 nm coherent, red laser light on sperm motility using a novel wavelet-based algorithm that allows for direct measurement of curvilinear velocity under red light illumination...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421619/molecular-detection-of-chlamydia-trachomatis-and-semen-quality-of-sexual-partners-of-infertile-women
#14
M López-Hurtado, M Velazco-Fernández, M J E Pedraza-Sánchez, V R Flores-Salazar, R Villagrana Zesati, F M Guerra-Infante
Chlamydia trachomatis is considered as the bacterium that is more sexually transmitted as cause of male urethritis, epididymitis, orchitis and infertility. A total of 116 semen samples of men whose couples are infertile women were analysed. The quality of the semen was measured by standard procedures recommended by WHO while C. trachomatis was detected by the PCR assay. Thirty-seven semen samples were positive for C. trachomatis (31.9%). Regarding semen analysis, no different values were observed between positive and negative samples to C...
April 19, 2017: Andrologia
https://www.readbyqxmd.com/read/28418610/localization-patterns-of-the-ganglioside-gm1-in-human-sperm-are-indicative-of-male-fertility-and-independent-of-traditional-semen-measures
#15
Cristina Cardona, Queenie V Neri, Alana J Simpson, Melissa A Moody, G Charles Ostermeier, Eric K Seaman, Theodore Paniza, Zev Rosenwaks, Gianpiero D Palermo, Alexander J Travis
Semen analysis lacks a functional component and best identifies extreme cases of infertility. The ganglioside GM1 is known to have functional roles during capacitation and acrosome exocytosis. Here, we assessed whether GM1 localization patterns (Cap-Score™) correspond with male fertility in different settings: Study 1 involved couples pursuing assisted reproduction in a tertiary care fertility clinic, while Study 2 involved men with known fertility versus those questioning their fertility at a local urology center...
April 18, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28414145/real-time-analysis-of-tenofovir-release-kinetics-using-quantitative-phosphorus-31-p-nuclear-magnetic-resonance-spectroscopy
#16
Vivek Agrahari, Jianing Meng, Sudhaunshu S Purohit, Nathan A Oyler, Bi-Botti C Youan
The dialysis method is classically used for drug separation before analysis, but, does not provide direct and real-time drug quantification and has limitations affecting the dialysis rate. In this study, a phosphorus nuclear magnetic resonance ((31)P-qNMR) method is developed for the real-time quantification of therapeutic molecules in vitro. The release kinetics of model drug tenofovir (TFV: anti-HIV microbicide) was analyzed in vaginal fluid simulant (VFS), semen fluid simulant (SFS), and human plasma (HP) from chitosan nanofibers (size ∼100-200nm) using the NMR (direct) method and compared with dialysis/UV-Vis (indirect) method...
April 13, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28410062/role-of-imaging-in-the-evaluation-of-male-infertility
#17
Pardeep K Mittal, Brent Little, Peter A Harri, Frank H Miller, Lauren F Alexander, Bobby Kalb, Juan C Camacho, Viraj Master, Matthew Hartman, Courtney C Moreno
Infertility is defined herein as the inability to achieve pregnancy after frequently engaging in unprotected sexual intercourse for 1 year. Among infertile couples, the cause of infertility involves the male partner in approximately 50% of cases. Male infertility is usually caused by conditions affecting sperm production, sperm function, or both, or blockages that prevent the delivery of sperm. Chronic health problems, injuries, lifestyle choices, anatomic problems, hormonal imbalances, and genetic defects can have a role in male infertility...
April 14, 2017: Radiographics: a Review Publication of the Radiological Society of North America, Inc
https://www.readbyqxmd.com/read/28408942/identification-of-tengfu-jiangya-tablet-target-biomarkers-with-quantitative-proteomic-technique
#18
Jingwen Xu, Yunlun Li, Shijun Zhang, Haiqiang Jiang, Nan Wang, Haiqing Lin
Tengfu Jiangya Tablet (TJT) is a well accepted antihypertension drug in China and its major active components were Uncaria total alkaloids and Semen Raphani soluble alkaloid. To further explore treatment effects mechanism of TJT on essential hypertension, a serum proteomic study was performed. Potential biomarkers were quantified in serum of hypertension individuals before and after taking TJT with isobaric tags for relative and absolute quantitation (iTRAQ) coupled two-dimensional liquid chromatography followed electrospray ionization-tandem mass spectrometry (2D LC-MS/MS) proteomics technique...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28407330/diffusion-weighted-and-magnetization-transfer-imaging-in-testicular-spermatogenic-function-evaluation-preliminary-results
#19
Huanjun Wang, Jian Guan, Jinhua Lin, Zhongwei Zhang, Shurong Li, Yan Guo, Huasong Cai
PURPOSE: To assess the use of functional magnetic resonance imaging (MRI), including diffusion-weighted MRI (DWI) and magnetization transfer MRI (MTI) in evaluating male infertility. MATERIALS AND METHODS: Sixteen men with testicular spermatogenesis hypofunction confirmed by percutaneous testis biopsy and 31 volunteers (control group B, age range: 20-40 years) with normal semen analysis including younger (By, n = 15, age range: 20-30 years) and older (Bo, n = 16, age range: 31-40 years) men underwent pelvic 3T MRI, including DWI and MTI...
April 13, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28400736/nicotine-effects-and-receptor-expression-on-human-spermatozoa-possible-neuroendocrine-mechanism
#20
Rosita A Condorelli, Sandro La Vignera, Filippo Giacone, Linda Iacoviello, Laura M Mongioì, Giovanni Li Volti, Ignazio Barbagallo, Roberto Avola, Aldo E Calogero
The aim of this experimental study was to investigate the mechanism by which nicotine (NIC) alters spermatozoa and to evaluate the expression of nicotinic receptors (nAChR) subunits in human spermatozoa. We analyzed 30 healthy normozoospermic men. Spermatozoa were incubated with NIC 100 ng/ml and the nAChR antagonist, hexamethonium (HEX) (0, 100, 1,000, 10,000 ng/ml) for 3 and 24 h. The following sperm parameters evaluated: (a) progressive motility; (b) mitochondrial membrane potential (MMP); (c) chromatin compactness; (d) externalization of phosphatidylserine (PS); (e) late apoptosis; (f) viability; (g) DNA fragmentation; (h) degree of lipid peroxidation (LP) by flow cytometry; (i) nAChR subunits expression by quantitative Real Time PCR and (j) protein expression evaluation by Western blot analysis...
2017: Frontiers in Physiology
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