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https://www.readbyqxmd.com/read/28650694/investigation-of-the-developmental-potential-and-developmental-kinetics-of-bovine-parthenogenetic-and-somatic-cell-nuclear-transfer-embryos-using-a-time-lapse-monitoring-system
#1
Min-Jee Park, Eun-Young Kim, Man-Jong Kang, Jun-Beom Lee, Chang-Jin Jeong, Se-Pill Park
A time-lapse monitoring system has predictive value for selecting good-quality embryos with the highest implantation potential. Using this new tool, we investigated the developmental potential and developmental kinetics of bovine parthenogenetic (PA) and two types of somatic cell nuclear transfer (NT) embryos. Bovine non-transgenic ear cells (bECs) or transgenic cells (bTGCs) were used as donor cells. The cleavage and blastocyst development rates did not significantly differ among the PA, NT-bEC, and NT-bTGC groups, and first cleavage occurred an average of 19...
June 26, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28648997/effects-tolerance-mechanisms-and-management-of-salt-stress-in-grain-legumes
#2
REVIEW
Muhammad Farooq, Nirmali Gogoi, Mubshar Hussain, Sharmistha Barthakur, Sreyashi Paul, Nandita Bharadwaj, Hussein M Migdadi, Salem S Alghamdi, Kadambot H M Siddique
Salt stress is an ever-present threat to crop yields, especially in countries with irrigated agriculture. Efforts to improve salt tolerance in crop plants are vital for sustainable crop production on marginal lands to ensure future food supplies. Grain legumes are a fascinating group of plants due to their high grain protein contents and ability to fix biological nitrogen. However, the accumulation of excessive salts in soil and the use of saline groundwater are threatening legume production worldwide. Salt stress disturbs photosynthesis and hormonal regulation and causes nutritional imbalance, specific ion toxicity and osmotic effects in legumes to reduce grain yield and quality...
June 16, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28646146/optical-coherence-microscopy-as-a-novel-non-invasive-method-for-the-4d-live-imaging-of-early-mammalian-embryos
#3
Karol Karnowski, Anna Ajduk, Bartosz Wieloch, Szymon Tamborski, Krzysztof Krawiec, Maciej Wojtkowski, Maciej Szkulmowski
Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers still limits researchers' ability to visualize the cellular architecture without causing short- and long-term photodamage. Optical coherence microscopy (OCM) is a promising alternative that circumvents the technical limitations of fluorescence imaging techniques and provides unique access to fundamental aspects of early embryonic development, without the requirement for sample pre-processing or labeling...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28645286/the-myths-surrounding-mild-stimulation-in-vitro-fertilization-ivf
#4
REVIEW
Raoul Orvieto, Valeria Stella Vanni, Norbert Gleicher
So-called mild controlled ovarian hyperstimulation (mCOH) has in recent years increased in popularity, claiming to be safer and more patient-friendly, while also improving in vitro fertilization (IVF) outcomes. We here challenge the International Society for Mild Approaches in Assisted Reproduction (ISMAAR) definition of mild stimulation, and especially address four fundamental issues, where our review found conventional COH (cCOH) advantageous over mCOH. They are: prevalence of severe ovarian hyperstimulation syndrome (OHSS), oocyte/embryo quality, pregnancy/live birth rates, and cost...
June 24, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28644873/circular-rna-expression-profiling-of-human-granulosa-cells-during-maternal-aging-reveals-novel-transcripts-associated-with-assisted-reproductive-technology-outcomes
#5
Jing Cheng, Jia Huang, Suzhen Yuan, Su Zhou, Wei Yan, Wei Shen, Yun Chen, Xi Xia, Aiyue Luo, Da Zhu, Shixuan Wang
Circular RNAs (circRNAs) are a unique class of endogenous RNAs which could be used as potential diagnostic and prognostic biomarkers of many diseases. Our study aimed to investigate circRNA profiles in human granulosa cells (GCs) during maternal aging and to uncover age-related circRNA variations that potentially reflect decreased oocyte competence. CircRNAs in GCs from in vitro fertilization (IVF) patients with young age (YA, ≤ 30 years) and advanced age (AA, ≥ 38 years) were profiled by microarray, and validated in 20 paired samples...
2017: PloS One
https://www.readbyqxmd.com/read/28643503/the-use-of-morphokinetic-as-a-predictor-of-implantation
#6
Alberto Tejera, Belén Aparicio-Ruiz, Marcos Meseguer
In recent years the increased efforts intended for improving future outcomes in the laboratory have focused mostly on the search of additional markers of embryo quality to add up present embryo selection criteria. Time-lapse system involves an alternative tool in assisted reproduction techniques, being able to improve the embryo selection from a dynamic and interactive approach while standard embryo assessment implies a subjective and static morphology evaluation and consequently reducing the information gained for embryo selection, time-lapse technology adds several morphokinetic parameters, providing additional input for embryo evaluation...
June 22, 2017: Minerva Ginecologica
https://www.readbyqxmd.com/read/28638275/the-application-of-dehydroepiandrosterone-on-improving-mitochondrial-function-and-reducing-apoptosis-of-cumulus-cells-in-poor-ovarian-responders
#7
Li-Te Lin, Peng-Hui Wang, Zhi-Hong Wen, Chia-Jung Li, San-Nung Chen, Eing-Mei Tsai, Jiin-Tsuey Cheng, Kuan-Hao Tsui
Poor ovarian responders (PORs) pose a great challenge for in vitro fertilization (IVF). Previous studies have suggested that dehydroepiandrosterone (DHEA) may improve IVF outcomes in PORs. The current study attempted to investigate the clinical benefits of DHEA in PORs and the possible mechanisms of DHEA on cumulus cells (CCs). This was a prospective study performed at one tertiary center from January 2015 to March 2016. A total of 131 women who underwent IVF treatment participated, including 59 normal ovarian responders (NORs) and 72 PORs...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28637468/chorioallantoic-membranes-of-embryonated-chicken-eggs-as-an-alternative-system-for-isolation-of-equine-influenza-virus
#8
Ilona Marcelina Gora, Malgorzata Kwasnik, Jan Franciszek Zmudzinski, Wojciech Rozek
BACKGROUND: Influenza virus isolation in embryonated chicken eggs (ECEs) is not applicable for rapid diagnosis, however it allows the recovery and propagation of the viable virus. A low number of infectious virus particles in the swabs, poor quality of samples or individual strain properties can lead to difficulties during the virus isolation process. We propose to utilize chorioallantoic membranes (CAM) of ECEs with the assistance of real-time RT PCR to facilitate equine influenza virus isolation...
June 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28635583/cigarette-smoke-is-associated-with-altered-expression-of-antioxidant-enzymes-in-granulosa-cells-from-women-undergoing-in-vitro-fertilization
#9
Maria Cristina Budani, Erminia Carletti, Gian Mario Tiboni
This study was undertaken to evaluate whether cigarette smoke is associated with changes in the expression of antioxidant enzymes in granulosa cells of women undergoing IVF treatments. For this aim, the expression of three antioxidant enzymes (SOD1, SOD2 and catalase) in non-smokers (n = 20) and smokers (n = 20) was analyzed. There was a statistically significant overexpression of SOD2 and catalase mRNA levels in smokers in comparison with non-smokers. Cigarette smoking was associated with a lower fertilization rate, implantation rate and pregnancy rate in comparison with non-smokers...
June 21, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28632990/shortening-gametes-co-incubation-time-improves-live-birth-rate-for-couples-with-a-history-of-fragmented-embryos
#10
Anne Le Bras, Laetitia Hesters, Vanessa Gallot, Cathie Tallet, Gerard Tachdjian, Nelly Frydman
Short gamete co-incubation (SGCO) consists in decreasing the duration of contact between oocytes and sperm from the standard overnight insemination (SOI) toward 2 hours. However, the effectiveness of this technique to improve in vitro fertilization and embryo transfer (IVF-ET) outcomes remains controversial. Our study was designed to evaluate the efficiency of SGCO in a poor prognosis population with a history of fragmented embryos defined by the presence of at least 50% of the embryos with more than 25% of cytoplasmic fragments...
June 20, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28631934/breast-milk-from-smokers-contains-less-cholesterol-and-protein-and-smaller-size-of-apolipoprotein-a-i-resulting-in-lower-zebrafish-embryo-survivability
#11
Seong-Min Kim, Suk-Jeong Kim, Jae-Yong Kim, Jae-Ryong Kim, Kyung-Hyun Cho
BACKGROUND: To determine the quality of breast milk (BM), we compared the functions of BM from ex-smokers and nonsmokers. SUBJECTS AND METHODS: We analyzed the contents of lipids, glucose, and protein in BM from ex-smokers (10 cigarettes/day for 13 ± 3 years) as well as infant formula. RESULTS: Nonsmokers' BM showed 2.4- and 1.4-fold higher cholesterol and protein contents, respectively, than BM from smokers. Infant formula contained almost no cholesterol, but did show remarkably higher glucose and triglyceride levels than BM...
June 20, 2017: Breastfeeding Medicine: the Official Journal of the Academy of Breastfeeding Medicine
https://www.readbyqxmd.com/read/28630101/oviduct-extracellular-vesicles-protein-content-and-their-role-during-oviduct-embryo-cross-talk
#12
Carmen Alminana, Emilie Corbin, Guillaume Tsikis, Agostinho S Alcantara-Neto, Valérie Labas, Karine Reynaud, Laurent Galio, Rustem Uzbekov, Anastasiia S Garanina, Xavier Druart, Pascal Mermillod
Successful pregnancy requires an appropriate communication between the mother and the embryo. Recently, exosomes and microvesicles, both membrane-bound extracellular vesicles (EVs) present in the oviduct fluid have been proposed as key modulators of this unique cross-talk. However, little is known about their content and their role during oviduct-embryo dialogue. Given the known differences in secretions by in vivo and in vitro oviduct epithelial cells (OEC), we aimed at deciphering the oviduct EVs protein content from both sources...
June 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28629355/biologically-constrained-optimization-based-cell-membrane-segmentation-in-c-elegans-embryos
#13
Yusuke Azuma, Shuichi Onami
BACKGROUND: Recent advances in bioimaging and automated analysis methods have enabled the large-scale systematic analysis of cellular dynamics during the embryonic development of Caenorhabditis elegans. Most of these analyses have focused on cell lineage tracing rather than cell shape dynamics. Cell shape analysis requires cell membrane segmentation, which is challenging because of insufficient resolution and image quality. This problem is currently solved by complicated segmentation methods requiring laborious and time consuming parameter adjustments...
June 19, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28627828/the-relationship-between-the-mthfr-c677t-genotypes-to-serum-anti-m%C3%A3-llerian-hormone-concentrations-and-in-vitro-fertilization-intracytoplasmic-sperm-injection-outcome
#14
Seyedeh Z Shahrokhi, Faranak Kazerouni, Firouzeh Ghaffari, Ali Rahimipour, Mir D Omrani, Arezoo Arabipoor, Ramin Lak, Elaheh T Ghane
BACKGROUND: The expression of the 5,10-methylentetrahydrofolate reductase (MTHFR) gene in human oocytes and preimplantation embryos suggests that the MTHFR gene is involved in folliculogenesis and female reproduction. Considering the importance of the MTHFR gene on female reproduction, the aim of this study was to evalu- ate the influence of MTHFR C677T polymorphism on ovarian marker reserve, particularly serum anti-Müllerian hormone (AMH) levels, and ovarian response as well as clinical pregnancy rates after in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI)...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28625274/beneficial-effects-of-melatonin-on-in-vitro-embryo-production-from-juvenile-goat-oocytes
#15
Sandra Soto-Heras, Montserrat Roura, Maria G Catalá, Irene Menéndez-Blanco, Dolors Izquierdo, Ali A Fouladi-Nashta, Maria T Paramio
Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57--1.07×10-9 M, increasing with follicular diameter. Oocytes were matured, fertilised and cultured under conventional conditions. Blastocyst development, embryo quality and levels of reactive oxygen species (ROS) and reduced glutathione were assessed...
June 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28623529/ameliorative-effect-of-omega-3-on-spermatogenesis-testicular-antioxidant-status-and-preimplantation-embryo-development-in-streptozotocin-induced-diabetes-in-rats
#16
Mehdi Khavarimehr, Vahid Nejati, Mazdak Razi, Gholamreza Najafi
PURPOSE: The present study was done to determine the ameliorative effect of polyunsaturated fatty acid omega-3 against experimental diabetes-induced damages on testicular tissue, sperm parameters and preimplantation embryo development in rat model. METHODS: Thirty-two mature male rats were divided into two control and test groups. The experimental diabetes (50 mg kg(-1) streptozotocin, ip) was induced in test group and subdivided into non-treated diabetic, 300 and 600 mg kg(-1) omega-3-treated (orally by gavage) groups...
June 16, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28622373/a-versatile-and-low-cost-open-source-pipetting-robot-for-automation-of-toxicological-and-ecotoxicological-bioassays
#17
Sebastian Steffens, Leonie Nüßer, Thomas-Benjamin Seiler, Nadine Ruchter, Mark Schumann, Ricarda Döring, Catrina Cofalla, Avi Ostfeld, Elad Salomons, Holger Schüttrumpf, Henner Hollert, Markus Brinkmann
In the past decades, bioassays and whole-organism bioassay have become important tools not only in compliance testing of industrial chemicals and plant protection products, but also in the monitoring of environmental quality. With few exceptions, such test systems are discontinuous. They require exposure of the biological test material in small units, such as multiwell plates, during prolonged incubation periods, and do not allow online read-outs. It is mostly due to these shortcomings that applications in continuous monitoring of, e...
2017: PloS One
https://www.readbyqxmd.com/read/28616498/xenotransplantation-a-special-case-of-one-health
#18
REVIEW
Joachim Denner
The chronic shortage of human transplants to treat tissue and organ failure has led to the development of xenotransplantation, the transplantation of cells, tissues and organs from another species to human recipients. For a number of reasons, pigs are best suited as donor animals. Successful, routine xenotransplantation would have an enormous impact on the health of the human population, including the young, who sometimes require a replacement organ or islet cells, but especially the elderly, who more often suffer the consequences of organ failure...
June 2017: One Health
https://www.readbyqxmd.com/read/28610639/impact-of-the-ivf-laboratory-environment-on-human-preimplantation-embryo-phenotype
#19
D K Gardner, R L Kelley
The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its morphology, developmental kinetics, physiology and metabolism, can be affected by numerous components of the laboratory and embryo culture system (which comprise the laboratory environment). The culture media formulation is important in determining embryo phenotype, but this exists within a culture system that includes oxygen, temperature, pH and whether an embryo is cultured individually or in a group, all of which can influence embryo development...
June 14, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28609268/key-performance-indicators-score-kpis-score-based-on-clinical-and-laboratorial-parameters-can-establish-benchmarks-for-internal-quality-control-in-an-art-program
#20
José G Franco, Claudia G Petersen, Ana L Mauri, Laura D Vagnini, Adriana Renzi, Bruna Petersen, M C Mattila, Vanessa A Comar, Juliana Ricci, Felipe Dieamant, João Batista A Oliveira, Ricardo L R Baruffi
OBJECTIVE: KPIs have been employed for internal quality control (IQC) in ART. However, clinical KPIs (C-KPIs) such as age, AMH and number of oocytes collected are never added to laboratory KPIs (L-KPIs), such as fertilization rate and morphological quality of the embryos for analysis, even though the final endpoint is the evaluation of clinical pregnancy rates. This paper analyzed if a KPIs-score strategy with clinical and laboratorial parameters could be used to establish benchmarks for IQC in ART cycles...
June 1, 2017: JBRA Assisted Reproduction
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