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Embryo quality

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https://www.readbyqxmd.com/read/28528417/sea-urchin-embryogenesis-as-bioindicators-of-marine-pollution-in-impact-areas-of-the-sea-of-japan-east-sea-and-the-sea-of-okhotsk
#1
Olga N Lukyanova, Elena V Zhuravel, Denis N Chulchekov, Andrey A Mazur
The embryogenesis of the sea urchin sand dollar Scaphechinus mirabilis was used as bioindicators of seawater quality from the impact areas of the Sea of Japan/East Sea (Peter the Great Bay) and the Sea of Okhotsk (northwestern shelf of Sakhalin Island and western shelf of Kamchatka Peninsula). Fertilization membrane formation, first cleavage, blastula formation, gastrulation, and 2-armed and 4-armed pluteus formation have been analyzed and a number of abnormalities were calculated. Number of embryogenesis anomalies in sand dollar larvae exposed to sea water from different stations in Peter the Great Bay corresponds to pollution level at each area...
May 20, 2017: Archives of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28528388/fertility-sparing-for-young-patients-with-gynecologic-cancer-how-mri-can-guide-patient-selection-prior-to-conservative-management
#2
REVIEW
Sinead H McEvoy, Stephanie Nougaret, Nadeem R Abu-Rustum, Hebert Alberto Vargas, Elizabeth A Sadowski, Christine O Menias, Fuki Shitano, Shinya Fujii, Ramon E Sosa, Joanna G Escalon, Evis Sala, Yulia Lakhman
Historically, cancer treatment has emphasized measures for the "cure" regardless of the long-term consequences. Advances in cancer detection and treatment have resulted in improved outcomes bringing to the fore various quality of life considerations including future fertility. For many young cancer patients, fertility preservation is now an integral component of clinical decision-making and treatment design. Optimal fertility-sparing options for young patients with gynecologic cancer are influenced by patient age, primary cancer, treatment regimens, and patient preferences...
May 20, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28526407/sirtuin-inhibition-leads-to-autophagy-and-apoptosis-in-porcine-preimplantation-blastocysts
#3
Min Gyeong Kim, Duk Hyoun Kim, Hye Ran Lee, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
Sirtuins are nicotinamide adenine dinucleotide dependent class III histone deacetylase proteins that play a crucial role in several cellular processes, including DNA repair, apoptosis, and lifespan. Previous studies have shown that sirtuin inhibition leads to embryonic developmental arrest and oxidative stress in porcine and murine. However, sirtuin-mediated mechanisms have not been examined in porcine preimplantation blastocysts. We therefore investigated the relationship between sirtuins and autophagy. Embryos were cultured with 100 μM sirtinol (SIRT1/2 inhibitor) in NCSU-23 media after in vitro fertilization...
May 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28523429/kir-lilrb-and-their-ligands-genes-as-potential-biomarkers-in-recurrent-implantation-failure
#4
REVIEW
Izabela Nowak, Karolina Wilczyńska, Jacek R Wilczyński, Andrzej Malinowski, Paweł Radwan, Michał Radwan, Piotr Kuśnierczyk
Reproductive failure in humans is a very important social and economic problem, because nowadays women decide to conceive later in life and delay motherhood. Unfortunately, with increasing age they have less chance for natural fertilization and maintenance of pregnancy. Many of them need assisted reproductive technology. Approximately 10% of women after in vitro fertilization-embryo transfers experience recurrent implantation failure (RIF). Multiple factors may contribute to RIF, including oocyte and sperm quality, parental chromosomal anomalies, genetic or metabolic abnormalities of the embryo, poor uterine receptivity, immunological disturbances in the implantation site, and some gynecologic pathologies such as endometriosis, uterine fibroids, hydrosalpinx and endometrial polyps...
May 18, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28520897/alpha-linolenic-acid-protects-the-developmental-capacity-of-bovine-cumulus-oocyte-complexes-matured-under-lipotoxic-conditions-in-vitro
#5
W F A Marei, J De Bie, O Mohey-Elsaeed, E Wydooghe, P E J Bols, J L M R Leroy
Elevated concentrations of free fatty acids (FFAs), predominantly palmitic, stearic and oleic acids (PSO), exert detrimental effects on oocyte developmental competence. This study examined the effects of omega-3 alpha-linolenic acid (ALA) during in vitro oocyte maturation (IVM) in the presence of PSO on subsequent embryo development and quality, and the cellular mechanisms that might be involved. Bovine cumulus oocyte complexes (COCs) were supplemented during IVM with ALA (50 μM), PSO (425 μM), or PSO+ALA...
May 18, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28520729/top-quality-blastocyst-formation-rates-in-relation-to-progesterone-levels-on-the-day-of-oocyte-maturation-in-gnrh-antagonist-ivf-icsi-cycles
#6
V S Vanni, E Somigliana, M Reschini, L Pagliardini, E Marotta, S Faulisi, A Paffoni, P Vigano', W Vegetti, M Candiani, E Papaleo
Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a "freeze-all" strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5-6...
2017: PloS One
https://www.readbyqxmd.com/read/28518097/light-sheet-based-fluorescence-microscopy-of-living-or-fixed-and-stained-tribolium-castaneum-embryos
#7
Frederic Strobl, Selina Klees, Ernst H K Stelzer
The red flour beetle Tribolium castaneum has become an important insect model organism in developmental genetics and evolutionary developmental biology. The observation of Tribolium embryos with light sheet-based fluorescence microscopy has multiple advantages over conventional widefield and confocal fluorescence microscopy. Due to the unique properties of a light sheet-based microscope, three dimensional images of living specimens can be recorded with high signal-to-noise ratios and significantly reduced photo-bleaching as well as photo-toxicity along multiple directions over periods that last several days...
April 28, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28513911/sperm-capacitation-pretreatment-positively-impacts-bovine-intracytoplasmic-sperm-injection
#8
L Águila, F Zambrano, M E Arias, Felmer R
The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is low compared to other species due in part to inadequate egg activation and sperm nucleus decondensation after injection. We hypothesized that this low efficiency is due to the lack of complete sperm capacitation, so we evaluated the effects of isobutylmethylxanthine (IBMX) and methyl-β-cyclodextrin (MβCD) on bovine sperm capacitation and on the preimplantation developmental potential of bovine embryos generated by ICSI. Treatment with IBMX and MβCD decreased sperm viability (between 13 to 30%); nevertheless, 0...
May 17, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28511712/what-we-learned-from-extended-culture-of-rejected-day-3-cleavage-stage-embryos-a-prospective-cohort-study
#9
Anat Hershko Klement, Michal Ovadia, Amir Wiser, Arie Berkovitz, Tal Shavit, Luba Nemerovsky, Yehudith Ghetler, Ilan Cohen, Adrian Shulman
BACKGROUND: To test whether poor quality day-3 embryos can undergo successful blastulation and implantation. METHODS: A prospective cohort study was conducted. Whether or not a good quality embryo was transferred on day-3, poor quality (rejected) embryos were further cultured and followed. The clinical outcome of each embryo was assessed. RESULTS: A total of 694 rejected embryos (from 205 patients) were included, with a blastulation rate of 21...
May 16, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28504820/using-propensity-score-matching-to-evaluate-the-effect-of-complementary-medicine-on-clinical-and-embryologic-outcomes-of-in-vitro-fertilization
#10
Anat Porat-Katz, Talia Eldar-Geva, Arik Kahane, Ora Paltiel
OBJECTIVE: To evaluate associations between using complementary medical therapies (CMTs) and embryologic and clinical outcomes of in vitro fertilization (IVF). METHODS: The present prospective questionnaire-based cohort study enrolled women aged 18-44 years undergoing their first, second, or third IVF cycle using their own oocytes at a large IVF clinic in Israel between February 1, 2013, and July 31, 2015. Clinical and embryologic data were obtained from patient records and patients completed a self-administered questionnaire that included details of any CMTs used to treat infertility...
May 15, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28498817/epigenetic-dynamics-and-interplay-during-spermatogenesis-and-embryogenesis-implications-for-male-fertility-and-offspring-health
#11
REVIEW
Shao-Qin Ge, Sheng-Li Lin, Zheng-Hui Zhao, Qing-Yuan Sun
Mapping epigenetic modifications and identifying their roles in the regulation of spermatogenesis and embryogenesis are essential for gaining fundamental medical understandings and for clinical applications. More and more evidence has shown that specific epigenetic modifications are established during spermatogenesis, which will be transferred into oocyte via fertilisation, and play an important role in the early embryo development. Defects in epigenetic patterns may increase the risk of abnormal spermatogenesis, fertilisation failure, early embryogenesis abnormality and several other complications during pregnancy...
April 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28485996/impact-of-hydrosalpinx-fluid-on-early-human-embryos
#12
Hongchu Bao, Qinglan Qu, Xin Huang, Meimei Wang, Xinrong Wang, Cuifang Hao
This study aimed to investigate the impact of hydrosalpinx fluid (HF) on early human embryonic development. A total of 33 patients who underwent laparoscopic surgery for hydrosalpinx were selected, and the HF specimens obtained from these patients were subjected to bacterial culture, Chlamydia antigen detection, biochemical analysis, and cytokine detection. Meanwhile, human embryos derived from three pronuclei (3PN) were cultured in various HF concentrations. There was no significant difference in the chemical components and physical characteristics between colorless and colored HF specimens, apart from the glucose concentration which was significantly higher in colorless HF...
May 9, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28479756/relationship-between-morphology-euploidy-and-implantation-potential-of-cleavage-and-blastocyst-stage-embryos
#13
Gaurav Majumdar, Abha Majumdar, Ishwar C Verma, Kailash C Upadhyaya
AIM: The aim of this study was to investigate the relationship between the morphology, euploidy and implantation rate of cleavage stage and blastocyst stage embryos. SETTING: Institution-based, tertiary care in-vitro fertilization centre. STUDY DESIGN: This study included a retrospective data analysis of 306 embryos: 154 cleavage stage embryos and 152 blastocysts that underwent biopsy on day 3 and day 5/6, respectively, which were subsequently screened for aneuploidy by array comparative genomic hybridization analysis...
January 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28479754/effect-of-body-mass-index-on-the-outcome-of-in-vitro-fertilization-intracytoplasmic-sperm-injection-in-women
#14
Manish Banker, Dipesh Sorathiya, Sandeep Shah
BACKGROUND: Obesity, a known epidemic, is a leading cause of various reproductive disorders. Association of body mass index (BMI) with pregnancy outcomes, either ovarian or endometrial, is controversial and least elucidated. AIM: This study aimed to analyze the effect of BMI on in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) outcome in women using self-oocytes, embryos prepared from donor oocytes (DE), or vitrified/frozen embryos (VE) obtained from both the SE and DE groups...
January 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28479126/low-serum-concentration-in-bovine-embryo-culture-enhances-early-blastocyst-rates-on-day-6-with-quality-traits-in-the-expanded-blastocyst-stage-similar-to-bsa-cultured-embryos
#15
A Murillo, M Muñoz, D Martín-González, S Carrocera, A Martínez-Nistal, E Gómez
In bovine, single in vitro embryo culture in protein-free medium from Day-6 to Day-7 leads to expanded blastocyst (XB) with improved pregnancy and birth rates after cryopreservation. Under these conditions, early blastocysts (EB) progress to the XB stage at higher rates than morulae (M). However, embryo production with BSA in culture prior to Day-6 leads to low EB rates. We investigated whether a very low FCS concentration (0.1%) in culture from Day-1 to Day-6 would improve EB rates and, subsequently, increase XB rates on Day-7 after single culture in protein-free medium...
May 4, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28477298/telomere-length-is-short-in-pcos-and-oral-contraceptive-does-not-affect-the-telomerase-activity-in-granulosa-cells-of-patients-with-pcos
#16
Ying Li, Bingbing Deng, Nengyong Ouyang, Ping Yuan, Lingyan Zheng, Wenjun Wang
PURPOSE: Our study aimed to investigate the association of telomerase activity (TA) and telomere length (TL) in granulosa cells (GCs) with IVF outcomes of polycystic ovary syndrome (PCOS) patients, and the effects of oral contraceptive pill (OCP) pretreatment on these two parameters. METHODS: One hundred sixty-three infertile women were enrolled and divided into a PCOS group (n = 65) and a non-PCOS group (n = 98). The PCOS group was further divided into an OCP pretreatment group (n = 35) and a non-OCP pretreatment group (n = 30), a TA <0...
May 6, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28476647/tctp-regulates-spindle-assembly-during-postovulatory-aging-and-prevents-deterioration-in-mouse-oocyte-quality
#17
Hyuk-Joon Jeon, Xiang-Shun Cui, Jing Guo, Jae Man Lee, Jae-Sung Kim, Jeong Su Oh
If no fertilization occurs for a prolonged time following ovulation, oocytes experience a time-dependent deterioration in quality both in vivo and in vitro due to processes called postovulatory aging. Because the postovulatory aging of oocytes has marked detrimental effects on embryo development and offspring, many efforts have been made to unveil the underlying mechanisms. Here we showed that translationally controlled tumor protein (TCTP) regulates spindle assembly during postovulatory aging and prevents deterioration in mouse oocyte quality...
May 2, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28476487/a-randomized-controlled-trial-comparing-the-efficacy-and-safety-of-two-hmg-preparations-gaining-their-lh-bioactivity-from-different-hcg-sources
#18
Gillian Lockwood, Barbara Cometti, Jeanette Bogstad, Karin Erb, Christian De Geyter, Janos Urbancsek, Silvia Trevisan, Khaled Pocate-Cheriet, Dominique de Ziegler
In this prospective, controlled, randomized, multicentre, non-inferiority study, efficacy and safety of two HMG preparations (Menopur(®)- Ferring and Meriofert®- IBSA Institut Biochimique SA) for ovarian stimulation were compared (270 women undergoing IVF aged between 18 and 39 years; BMI 30 kg/m(2) or less; less than three prior completed assisted reproduction technique cycles). A standard long down-regulation with gonadotrophin-releasing hormone agonist protocol, with HCG triggering was used; primary end-point was total number of oocytes retrieved; attention was paid toovarian hyperstimulation syndrome (OHSS)...
April 20, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28475432/obesity-and-ultrasound-estimated-visceral-fat-deposits-in-women-undergoing-assisted-reproductive-technology-art-procedures
#19
Andrea Ciavattini, Nina Montik, Nicolò Clemente, Fabrizia Santoni, Lorenzo Moriconi, Matteo Serri, Pamela Barbadoro, Jacopo Sabbatinelli, Arianna Vignini
The aim of this study was to evaluate the influence of body mass index (BMI) and ultrasound-estimated visceral adipose tissue deposits on oocyte quality and pregnancy rate in women undergoing Assisted Reproductive Technology (ART) procedures. The study included 58 women who underwent ART procedures. According to their BMI, the women were divided into normal weight and overweight/obese; an ultrasound evaluation of preperitoneal fat thickness (PFT) was also performed for each patient. The oocyte quality was then assessed, and samples of follicular fluid were collected from each woman, in order to evaluate the intrafollicular concentration of reactive oxygen species (ROS) as markers of oxidative stress and pro-inflammatory cytokines (IL-1β and IL-6) as markers of chronic inflammation...
May 5, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28475125/melatonin-promotes-the-in-vitro-development-of-microinjected-pronuclear-mouse-embryos-via-its-anti-oxidative-and-anti-apoptotic-effects
#20
Xiuzhi Tian, Feng Wang, Lu Zhang, Pengyun Ji, Jing Wang, Dongying Lv, Guangdong Li, Menglong Chai, Zhengxing Lian, Guoshi Liu
CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) combined with pronuclear microinjection has become the most effective method for producing transgenic animals. However, the relatively low embryo developmental rate limits its application. In the current study, it was observed that 10(-7) M melatonin is considered an optimum concentration and significantly promoted the in vitro development of murine microinjected pronuclear embryos, as indicated by the increased blastocyst rate, hatching blastocyst rate and blastocyst cell number...
May 5, 2017: International Journal of Molecular Sciences
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