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https://www.readbyqxmd.com/read/29352716/-echographic-diagnosis-of-missed-early-miscarriage-assessment-of-image-quality
#1
C Barthes, C Mezan De Malartic, C Baumann, H Rousseaux, O Morel
OBJECTIVE: Ultrasound examination plays a central role in case of suspected non-viable pregnancy. A wrong diagnosis might have major consequence in terms of inadequate care, especially in cases of false positive non-viable pregnancy diagnosis. Ultrasound criterions are today well defined. Our objective was to evaluate the feasibility and reproducibility of a novel image-quoting method of first-trimester non-viable pregnancy. METHODS: Thirty images of non-viable pregnancy were twice evaluated with blinded proofreading...
January 15, 2018: Gynecologie, Obstetrique, Fertilite & Senologie
https://www.readbyqxmd.com/read/29345445/the-impact-of-high-progesterone-levels-on-the-day-of-hcg-administration-in-assisted-human-reproduction-treatments
#2
Michelli S Tanada, Ivan H Yoshida, Monise Santos, Caroline Z Berton, Elen Souto, Waldemar P de Carvalho, Emerson B Cordts, Caio P Barbosa
OBJECTIVE: Progesterone is a steroid hormone that acts on the endometrium. It is known for producing physical and mood-related side effects. Few studies have looked into how progesterone levels affect embryo development and quality. This study aimed to find a cutoff level for serum progesterone on the day of HCG administration from which embryo quality is impaired. METHODS: The study included 145 cycles, from which 885 oocytes and 613 embryos were obtained. All patients had their serum progesterone levels measured on the day of HCG administration...
January 18, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29345310/a-potential-role-of-knockout-serum-replacement-as-a-porcine-follicular-fluid-substitute-for-in-vitro-maturation-lipid-metabolism-approach
#3
Jun-Xue Jin, Sanghoon Lee, Erif Maha Nugraha Setyawan, Anukul Taweechaipaisankul, Geon A Kim, Ho Jae Han, Curie Ahn, Byeong Chun Lee
The use of supplements, such as porcine follicular fluid (pFF), fetal bovine serum and human serum albumin are widely used during in vitro maturation (IVM) in different species but these supplements contain undefined components that cause technical difficulties in standardization and influence the efficiency of IVM. Knockout serum replacement (KSR) is a synthetic protein source, without any undefined growth factors or differentiation-promoting factors. Therefore, it is feasible to use KSR as a defined component for avoiding effects of unknown molecules in an IVM system...
January 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29339017/reliability-and-agreement-on-embryo-assessment-5-years-of-an-external-quality-control-programme
#4
Luis Martínez-Granados, María Serrano, Antonio González-Utor, Nereyda Ortiz, Vicente Badajoz, María Luisa López-Regalado, Montserrat Boada, Jose A Castilla
An external quality-control programme for morphology-based embryo quality assessment, incorporating a standardized embryo grading scheme, was evaluated over a period of 5 years to determine levels of inter-observer reliability and agreement between practising clinical embryologists at IVF centres and the opinions of a panel of experts. Following Guidelines for Reporting Reliability and Agreement Studies, the Gwet index and proportion of positive (Ppos) and negative agreement were calculated. For embryo morphology assessment, a substantial degree of reliability was measured between the centres and the panel of experts (Gwet index: 0...
December 29, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29337117/xist-derepression-in-active-x-chromosome-hinders-pig-somatic-cell-nuclear-transfer
#5
Degong Ruan, Jiangyun Peng, Xiaoshan Wang, Zhen Ouyang, Qingjian Zou, Yi Yang, Fangbing Chen, Weikai Ge, Han Wu, Zhaoming Liu, Yu Zhao, Bentian Zhao, Quanjun Zhang, Chengdan Lai, Nana Fan, Zhiwei Zhou, Qishuai Liu, Nan Li, Qin Jin, Hui Shi, Jingke Xie, Hong Song, Xiaoyu Yang, Jiekai Chen, Kepin Wang, Xiaoping Li, Liangxue Lai
Pig cloning by somatic cell nuclear transfer (SCNT) remains extremely inefficient, and many cloned embryos undergo abnormal development. Here, by profiling transcriptome expression, we observed dysregulated chromosome-wide gene expression in every chromosome and identified a considerable number of genes that are aberrantly expressed in the abnormal cloned embryos. In particular, XIST, a long non-coding RNA gene, showed high ectopic expression in abnormal embryos. We also proved that nullification of the XIST gene in donor cells can normalize aberrant gene expression in cloned embryos and enhance long-term development capacity of the embryos...
January 10, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29335812/sperm-telomere-length-in-donor-samples-is-not-related-to-icsi-outcome
#6
Marc Torra-Massana, Montserrat Barragán, Emanuela Bellu, Rafael Oliva, Amelia Rodríguez, Rita Vassena
PURPOSE: Variations in sperm telomere length (STL) have been associated with altered sperm parameters, poor embryo quality, and lower pregnancy rates, but for normozoospermic men, STL relevance in IVF/ICSI is still uncertain. Moreover, in all studies reported so far, each man's STL was linked to the corresponding female partner characteristics. Here, we study STL in sperm donor samples, each used for up to 12 women, in order to isolate and determine the relationship between STL and reproductive outcomes...
January 16, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29334199/comparison-of-cabergoline-and-quinagolide-in-prevention-of-severe-ovarian-hyperstimulation-syndrome-among-patients-undergoing-intracytoplasmic-sperm-injection
#7
Robabeh Taheripanah, Mahshid Vasef, Marzieh Zamaniyan, Anahita Taheripanah
Background: The aim of the current study is to compare quinagolide with cabergoline in prevention of ovarian hyperstimulation syndrome (OHSS) among high risk women undergoing intracytoplasmic sperm injection (ICSI). MATERIALS AND METHODS: This randomized clinical trial study was performed from March 2015 to February 2017. One hundred and twenty six women undergoing ICSI who were at high risk of developing OHSS (having over 20 follicles of >12 mm), were randomized into two groups...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29334036/short-term-storage-of-the-oocytes-affects-the-ploidy-status-in-the-yellowtail-tetra-astyanax-altiparanae
#8
Matheus Pereira Dos Santos, Nivaldo Ferreira do Nascimento, George Shigueki Yasui, Nycolas Levy Pereira, Takafumi Fujimoto, José Augusto Senhorini, Laura Satiko Okada Nakaghi
In fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae. Oocytes were stored in Dulbecco's phosphate-buffered saline (PBS) at 26°C, then aliquots were fertilized immediately after extrusion (control) and also after 60, 120, 180, and 240 min of storage...
January 15, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29333568/comparison-of-effects-of-thawing-entire-donor-sperm-vial-vs-partial-thawing-shaving-on-sperm-quality
#9
Micha Baum, Raoul Orvieto, Sapir Kon, Ronit Machtinger, Gil M Yerushalmi, Ariel Hourvitz
PURPOSE: Partial thawing of a vial of cryopreserved sperm (shaving) is sometimes applied as a measure to preserve sperm for further use, particularly in cases of very restricted sperm quantity. However, mechanical violence may disrupt the sperm-wall and lead to impaired in-vitro fertilization (IVF) outcomes. MATERIAL AND METHODS: In a retrospective case-control study at a tertiary, university-affiliated medical center, we compared the IVF/intracytoplasmic sperm injection (ICSI) outcomes of patients who used donor sperm following partial thawing (shaving) of the vial of cryopreserved sperm (n = 99) to a control group consisting of patients for whom the vial of sperm was completely thawed before use (n = 99)...
January 15, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29332610/improvements-in-the-conception-rate-milk-composition-and-embryo-quality-of-rabbit-does-after-dietary-enrichment-with-n-3-polyunsaturated-fatty-acids
#10
M Rodríguez, R M García-García, M Arias-Álvarez, P Millán, N Febrel, N Formoso-Rafferty, J López-Tello, P L Lorenzo, P G Rebollar
This work attempts to confirm the effect of an enriched diet with n-3 polyunsaturated fatty acids (PUFA) trying to mitigate the reproductive performances issues such as low conception rate of primiparous rabbits. A total of 127 does were fed ad libitum throughout their two first cycles with two diets with different fat sources: mixed fat in the control and salmon oil in the enriched one, with 3.19 g/100 g (n=63 does) and 28.77 g/100 g (n=64 does) of n-3 of the total fatty acid, respectively. Feed intake was similar between groups (P>0...
January 15, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29329387/patients-with-acephalic-spermatozoa-syndrome-linked-to-sun5-mutations-have-a-favorable-pregnancy-outcome-from-icsi
#11
Jianzheng Fang, Jingjing Zhang, Fuxi Zhu, Xiaoyu Yang, Yugui Cui, Jiayin Liu
STUDY QUESTION: Are Sad1 and UNC84 domain containing 5 (SUN5) mutations associated with the outcomes of ICSI in patients with acephalic spermatozoa syndrome (ASS)? SUMMARY ANSWER: Despite highly abnormal sperm morphology, ASS patients with SUN5 mutations have a favorable pregnancy outcome following ICSI. WHAT IS KNOWN ALREADY: ASS is a rare cause of infertility characterized by the production of a majority of headless spermatozoa, along with a small proportion of intact spermatozoa with an abnormal head-tail junction...
January 10, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29317175/premature-progesterone-rise-in-art-cycles
#12
REVIEW
B Lawrenz, L Melado, H M Fatemi
Premature rise of progesterone during the late follicular phase in stimulated IVF cycles is a frequent event and its effect on the endometrial receptivity and on the ART (Assisted Reproductive Technique) - outcome has become a matter of intense debate and research. An emerging body of evidence demonstrates that premature progesterone rise does have a negative impact on the outcome of the ART-success. Until now, the exact cause of progesterone elevation is not fully clear, however lately published studies points to the fact, that premature progesterone elevation might be caused by enhanced FSH stimulation...
January 6, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29315421/development-of-a-new-comprehensive-and-reliable-endometrial-receptivity-map-er-map-er-grade-based-on-rt-qpcr-gene-expression-analysis
#13
M Enciso, J P Carrascosa, J Sarasa, P A Martínez-Ortiz, S Munné, J A Horcajadas, J Aizpurua
STUDY QUESTION: Is it possible to determine the receptivity status of an endometrium by combined quantitative reverse transcription PCR (RT-qPCR) expression analysis of genes involved in endometrial proliferation and immunity? SUMMARY ANSWER: The new ER Map®/ER Grade® test can predict endometrial receptivity status by RT-qPCR using a new panel of genes involved in endometrial proliferation and the maternal immune response associated to embryonic implantation. WHAT IS KNOWN ALREADY: The human endometrium reaches a receptive status adequate for embryonic implantation around Days 19-21 of the menstrual cycle...
January 5, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29310072/in-situ-embryo-toxicity-test-with-sea-urchin-development-of-exposure-chamber-for-test-execution
#14
Lorenzo Morroni, Silvia Giuliani, David Pellegrini, Davide Sartori
In situ toxicity tests represent a technique rarely performed owing to the lack of standard methodologies or to technical or economic problems. Nevertheless, its application would allow for a more realistic interpretation of pollution effects than those obtained by standard laboratory tests. The goal of this study is to develop and validate a specific exposure chamber for in situ exposition of Paracentrotus lividus embryos to obtain a defined methodology to perform the sea urchin embryo toxicity test in field conditions...
December 27, 2017: Chemosphere
https://www.readbyqxmd.com/read/29308626/evaluating-the-effect-of-melatonin-on-has2-and-pgr-expression-as-well-as-cumulus-expansion-and-fertility-potential-in-mice
#15
Maryam Ezzati, Leila Roshangar, Jafar Soleimani Rad, Nahid Karimian
OBJECTIVES: Infertility is a worldwide health problem which affects approximately 15% of sexually active couples. One of the factors influencing the fertility is melatonin. Also, protection of oocytes and embryos from oxidative stress inducing chemicals in the culture medium is important. The aim of the present study was to investigate if melatonin could regulate hyaluronan synthase-2 (HAS2) and Progesterone receptor (PGR) expressions in the cumulus cells of mice oocytes and provide an in vitro fertilization (IVF) approach...
April 2018: Cell Journal
https://www.readbyqxmd.com/read/29308624/the-effects-of-in-vitro-maturation-technique-on-the-expression-of-genes-involved-in-embryonic-genome-activation-of-human-embryos
#16
Parvin Dorfeshan, Marefat Ghaffari Novin, Mohammad Salehi, Reza Masteri Farahani, Fatemeh Fadaei-Fathabadi, Ronak Sehatti
OBJECTIVES: In vitro maturation technique (IVM) is shown to have an effect on full maturation of immature oocytes and the subsequent embryo development. Embryonic genome activation (EGA) is considered as a crucial and the first process after fertilization. EGA failure leads to embryo arrest and possible implantation failure. This study aimed to determine the role of IVM in EGA-related genes expression in human embryo originated from immature oocytes and recovered from women receiving gonadotrophin treatment for assisted reproduction...
April 2018: Cell Journal
https://www.readbyqxmd.com/read/29307391/variables-associated-with-mitochondrial-copy-number-in-human-blastocysts-what-can-we-learn-from-trophectoderm-biopsies
#17
Maria José de Los Santos, Antonio Diez Juan, Amparo Mifsud, Amparo Mercader, Marcos Meseguer, Carmen Rubio, Antonio Pellicer
OBJECTIVE: To study the potential variables that affect the mitochondrial DNA (mtDNA) content of trophectoderm (TE) cells in blastocysts that have undergone TE biopsy. DESIGN: Observational retrospective single-center analysis. SETTING: University-affiliated private in vitro fertilization center. PATIENT(S): A total of 465 consecutive preimplantation genetic screening (PGS) cycles of 402 women undergoing preimplantation genetic testing...
January 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29305844/progesterone-rise-on-hcg-day-is-negatively-correlated-with-ivf-et-outcomes-in-natural-cycles
#18
Xiadi Wu, Yundong Mao, Yan Gao, Xiaoqiao Qian, Wei Wang, Wei Ding, Shi-Wen Jiang, Jiayin Liu
OBJECTIVE: To investigate the effect of progesterone rise on hCG day on the laboratory and clinical outcomes in natural cycles and to explore the possible factors related to the occurrence of progesterone elevation. PATIENTS AND STUDY METHODS: Retrospective analysis was performed in 1157 infertile women with decreased ovarian reserve. Eligible infertile women undergoing IVF in natural cycles were assigned to four groups according to serum progesterone levels on the day of hCG administration: group 1, P<2nmol/L; group 2, 2<P<2...
January 3, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29303234/platelet-rich-plasma-as-an-adjuvant-in-the-endometrial-preparation-of-patients-with-refractory-endometrium
#19
Aixa Molina, Jose Sánchez, William Sánchez, Vanessa Vielma
OBJECTIVE: To improve endometrial quality and implantation rates after the administration of platelet-rich plasma in patients with refractory endometrium. METHODS: 19 patients undergoing in vitro fertilization, aged between 33 and 45 years with a history of refractory endometrium, to whom platelet rich plasma was given by infusion with a catheter into the uterine cavity on the tenth day of the hormone replacement therapy, and then 72 hours after the first administration...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29303233/high-percentages-of-embryos-with-21-18-or-13-trisomy-are-related-to-advanced-paternal-age-in-donor-egg-cycles
#20
Javier García-Ferreyra, Roly Hilario, Julio Dueñas
OBJECTIVE: Advanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes. METHODS: A total of 378 embryos, obtained from 52 IVF/ICSI cycles with donated oocytes in conjunction with PGD, were allocated according to paternal age in three groups: Group A: ≤39 years (n=115 embryos), Group B: 40-49 years (n=157 embryos) and Group C: ≥50 year (n=106 embryos)...
January 5, 2018: JBRA Assisted Reproduction
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