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https://www.readbyqxmd.com/read/27923447/amelioration-of-non-alcoholic-fatty-liver-disease-with-npc1l1-targeted-igy-or-n-3-polyunsaturated-fatty-acids-in-mice
#1
Jin-Sik Bae, Jong-Min Park, Junghoon Lee, Byung-Chul Oh, Sang-Ho Jang, Yun Bin Lee, Young-Min Han, Chan-Young Ock, Ji-Young Cha, Ki-Baik Hahm
Patients with non-alcoholic fatty liver disease (NAFLD) have an increased risk for progression to hepatocellular carcinoma in addition to comorbidities such as cardiovascular and serious metabolic diseases; however, the current therapeutic options are limited. Based on our previous report that omega-3 polyunsaturated fatty acids (n-3 PUFAs) can significantly ameliorate high fat diet (HFD)-induced NAFLD, we explored the therapeutic efficacy of n-3 PUFAs and N-IgY, which is a chicken egg yolk-derived IgY specific for the Niemann-Pick C1-Like 1 (NPC1L1) cholesterol transporter, on NAFLD in mice...
January 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/27920191/effect-of-in-ovo-injection-of-raffinose-on-growth-performance-and-gut-health-parameters-of-broiler-chicken
#2
J D Berrocoso, R Kida, A K Singh, Y S Kim, R Jha
The effects of in ovo injection of raffinose (RFO) as a prebiotic on growth performance, relative weight of proventriculus, gizzard, drumstick and breast muscles, and ileum mucosa morphology were examined in Cobb 500 broilers. A total of 240 fertilized eggs were divided into 4 groups: a non-injected with intact shell and 3 levels of RFO solution (1.5, 3.0, and 4.5 mg in 0.2 mL of an aqueous diluents). The RFO solution was injected into the air sac on d 12 of incubation. In total 144 birds were fed a standard diet and management and sacrificed at d 21 post hatch for collection of samples...
December 5, 2016: Poultry Science
https://www.readbyqxmd.com/read/27920190/evaluation-of-chlorine-dioxide-based-product-as-a-hatchery-sanitizer
#3
P Maharjan, S Cox, U Gadde, F D Clark, K Bramwell, S E Watkins
Formaldehyde is commonly used to overcome contaminants introduced by hatching eggs or water supply in the hatcher cabinets. However, health risks associated with its use make economical alternatives important. This project evaluated a chlorine dioxide based product (CDBP) (0.3% concentrate) as a hatchery sanitizer in decontaminating microbial populations on the shell surface of hatching eggs (>18 d old), as well as its impact on hatchability and chick performance. Hatchers (0.20 m(2)) designed to hold approximately 50 eggs and equipped with circulation fans, heaters, and thermostats were used for the evaluation...
December 5, 2016: Poultry Science
https://www.readbyqxmd.com/read/27915245/the-yeast-tum1-affects-production-of-hydrogen-sulfide-from-cysteine-treatment-during-fermentation
#4
Chien-Wei Huang, Michelle Walker, Bruno Fedrizzi, Miguel Roncoroni, Richard Gardner, Vladimir Jiranek
The undesirable rotten-egg odour of hydrogen sulfide (H2S) produced by yeast shortly after yeast inoculation of grape musts, might be an important source of desirable varietal thiols, which contribute to tropical aromas in varieties such as Sauvignon Blanc. In this study, we observed that Saccharomyces cerevisiae strains produce an early burst of H2S from cysteine. Both Δmet2 and Δmet17 strains produce a larger burst, likely because they are unable to utilise the H2S in the sulfate assimilation pathway. For the first time, we show that TUM1 is partly responsible for the early production of H2S from cysteine...
December 2, 2016: FEMS Yeast Research
https://www.readbyqxmd.com/read/27914210/randomized-controlled-trial-of-oral-immunotherapy-for-egg-allergy-in-japanese-patients
#5
Masayuki Akashi, Hiroki Yasudo, Masami Narita, Ichiro Nomura, Akira Akasawa, Motohiro Ebisawa, Takao Takahashi, Yukihiro Ohya
BACKGROUND: Egg allergy is one of the most common food allergies in young child. While oral immunotherapy (OIT) is not routinely recommended in current guidelines, it has been considered as a potential alternative treatment strategy. Studies on OIT for food allergies have been explored; however, no studies with control group have been reported in Japan. METHODS: The first oral food challenge test (OFC) was performed on participants before treatment to ensure the diagnosis and evaluate the threshold dose for egg by the way of double-blind, placebo-controlled food challenge (DBPCFC)...
December 3, 2016: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/27911903/transfected-babesia-bovis-expressing-a-tick-gst-as-a-live-vector-vaccine
#6
Daiane P Oldiges, Jacob M Laughery, Nelson Junior Tagliari, Ronaldo Viana Leite Filho, William C Davis, Itabajara da Silva Vaz, Carlos Termignoni, Donald P Knowles, Carlos E Suarez
The Rhipicephalus microplus tick is a notorious blood-feeding ectoparasite of livestock, especially cattle, responsible for massive losses in animal production. It is the main vector for transmission of pathogenic bacteria and parasites, including Babesia bovis, an intraerythrocytic apicomplexan protozoan parasite responsible for bovine Babesiosis. This study describes the development and testing of a live B. bovis vaccine expressing the protective tick antigen glutathione-S-transferase from Haemaphysalis longicornis (HlGST)...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27906670/stag3-regulates-microtubule-stability-to-maintain-euploidy-during-mouse-oocyte-meiotic-maturation
#7
Mianqun Zhang, Xiaoxin Dai, Yalu Sun, Yajuan Lu, Changyin Zhou, Yilong Miao, Ying Wang, Bo Xiong
Stag3, a meiosis-specific subunit of cohesin complex, has been demonstrated to function in both male and female reproductive systems in mammals. However, its roles during oocyte meiotic maturation have not been fully defined. In the present study, we report that Stag3 uniquely accumulates on the spindle apparatus and colocalizes with microtubule fibers during mouse oocyte meiotic maturation. Depletion of Stag3 by gene-targeting morpholino disrupts normal spindle assembly and chromosome alignment in oocytes...
November 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27905284/parasites-determined-by-fecal-examination-in-horses-in-erzurum
#8
Hamza Avcıoğlu, Esin Güven, İbrahim Balkaya, Şevki Yavuz, Uğur Abay, Muzaffer Akyüz, Ömer Eltas
OBJECTIVE: This study aimed to determine the parasites present in horses belonging to the Erzurum Province. METHODS: Fecal samples were collected from 76 horses of different ages, genders and breeds in Erzurum. Individual fecal samples were collected and examined by flotation and sedimentation methods. RESULTS: The following species were detected: strongylid egg (57.89%), Parascaris equorum (10.52%), Dicrocoelium dendriticum (2.63%), Fasciola spp...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27903459/true-rifampicin-resistance-missed-by-the-mgit-prevalence-of-this-pheno-genotype-in-the-uk-and-ireland-after-18-month-surveillance
#9
Ximena Gonzalo, Pauline Claxton, Tim Brown, Lorraine Montgomery, Margaret Fitzgibbon, Ian Laurenson, Francis Drobniewski
OBJECTIVES: To characterise rifampicin resistant strains missed by the MGIT 960 system but not by egg-based media in the UK and Ireland and to ascertain their prevalence. METHODS: All strains sent for second line susceptibility testing were prospectively collected. Drug Susceptibility Testing was performed by Resistance Ratio (RR), Proportion Method (PM), MGIT 960 and MIC determination by microdilution. Rifampicin resistance conferring mutations were detected with line probe assays and sequencing...
November 26, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27903284/gp50-as-a-promising-early-diagnostic-antigen-for-taenia-multiceps-infection-in-goats-by-indirect-elisa
#10
Xing Huang, Jing Xu, Yu Wang, Cheng Guo, Lin Chen, Xiaobin Gu, Weimin Lai, Xuerong Peng, Guangyou Yang
BACKGROUND: Coenurosis is caused by coenurus, the metacestode of Taenia multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early coenurus infection. METHODS: In this study, we identified a full-length cDNA that encodes GP50 (TmGP50) from the transcriptome of T. multiceps, and then cloned and expressed in E. coli. The native proteins in adult stage and coenurus were located via immunofluorescence assays, while the potential of recombinant TmGP50 protein (rTmGP50) for indirect ELISA-based serodiagnostics was assessed using native goat sera...
December 1, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27899156/echinococcus-multilocularis-in-foxes-and-raccoon-dogs-an-increasing-concern-for-baltic-countries
#11
Guna Bagrade, Gunita Deksne, Zanda Ozoliņa, Samantha Jane Howlett, Maria Interisano, Adriano Casulli, Edoardo Pozio
BACKGROUND: In Europe, the life-cycle of Echinococcus multilocularis is predominantly sylvatic, involving red foxes (Vulpes vulpes) as the main definitive hosts and rodents such as muskrats and arvicolids as intermediate hosts. The parasite is the etiological agent of human alveolar echinococcosis, a malignant zoonotic disease caused by the accidental ingestion of eggs shed by definitive hosts in their faeces. The aims of this study were to investigate the prevalence of E. multilocularis in red foxes and raccoon dogs (Nyctereutes procyonoides) and to study the environmental factors favouring the perpetuation of the parasite in Latvia...
November 29, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27899131/support-for-targeted-sampling-of-red-fox-vulpes-vulpes-feces-in-sweden-a-method-to-improve-the-probability-of-finding-echinococcus-multilocularis
#12
Andrea L Miller, Gert E Olsson, Sofia Sollenberg, Moa Skarin, Helene Wahlström, Johan Höglund
BACKGROUND: Localized concentrations of Echinococcus multilocularis eggs from feces of infected red fox (Vulpes vulpes) can create areas of higher transmission risk for rodent hosts and possibly also for humans; therefore, identification of these areas is important. However, in a low prevalence environment, such as Sweden, these areas could be easily overlooked. As part of a project investigating the role of different rodents in the epidemiology of E. multilocularis in Sweden, fox feces were collected seasonally from rodent trapping sites in two regions with known parasite status and in two regions with unknown parasite status, 2013-2015...
November 29, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27894885/metabolic-rates-are-elevated-and-influenced-by-maternal-identity-during-the-early-yolk-dependent-post-hatching-period-in-an-estuarine-turtle-the-diamondback-terrapin-malaclemys-terrapin
#13
Christopher L Rowe, Ryan J Woodland, Sarah A Funck
Non-genetic maternal effects, operating through a female's physiology or behavior, can influence offspring traits and performance. Here we examined potential maternal influences on metabolic rates (MR) of offspring in an estuarine turtle, the diamondback terrapin (Malaclemys terrapin). Females and their eggs were collected from the field and the eggs incubated in the laboratory for subsequent measurement of MR of females, late-stage embryos, newly-hatched individuals that were nutritionally dependent on yolk, and older hatchlings that had depleted their yolk reserves and thus were independent of energetic contributions from the female...
November 25, 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27890084/comparison-of-molecular-and-conventional-methods-for-the-diagnosis-of-fasciola-hepatica-infection-in-the-field
#14
Maria Immaculata Arifin, Johan Höglund, Adam Novobilský
The liver fluke, Fasciola hepatica, is one of the major parasite threats to livestock industries world-wide. In sheep and cattle, F. hepatica infection is commonly diagnosed using a range of methods. Aside from conventional coprological and serological diagnostic methods, there are also several molecular methods available based on the detection of liver fluke DNA in faeces. In this study, the outcomes of faecal egg count (FEC), serology and coproantigen ELISA (cELISA) were compared with the performance of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) in diagnosis of F...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27887877/a-discrete-stage-structured-model-of-california-newt-population-dynamics-during-a-period-of-drought
#15
Marjorie T Jones, William R Milligan, Lee B Kats, Thomas L Vandergon, Rodney L Honeycutt, Robert N Fisher, Courtney L Davis, Timothy A Lucas
We introduce a mathematical model for studying the population dynamics under drought of the California newt (Taricha torosa), a species of special concern in the state of California. Since 2012, California has experienced a record-setting drought, and multiple studies predict drought conditions currently underway will persist and even increase in severity. Recent declines and local extinctions of California newt populations in Santa Monica Mountain streams motivate our study of the impact of drought on newt population sizes...
November 22, 2016: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/27886036/prevalence-assessment-and-antimicrobial-resistance-patterns-of-salmonella-from-raw-chicken-eggs-in-haramaya-ethiopia
#16
Jelalu Kemal, Berhanu Sibhat, Sissay Menkir, Desta Beyene
INTRODUCTION: The presence of antimicrobial-resistant Salmonella in poultry and poultry products, including eggs, is a global public health concern. This study aimed to estimate the levels and patterns of antimicrobial resistance of Salmonella from chicken eggs and assess consumers' raw egg consumption and farmers' handling practices. METHODOLOGY: A total of 300 egg samples were collected from Haramaya open market (n = 150) and Haramaya University poultry farm (n = 150) in Ethiopia...
November 24, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27885744/glutathione-in-combination-with-trehalose-has-supplementary-beneficial-effects-on-cryopreserved-red-deer-cervus-elaphus-sperm
#17
Yan Wang, Shude Dong
OBJECTIVES: In this study, we evaluated the effects of glutathione in combination with trehalose addition to semen extenders on the quality parameters of frozen-thawed red deer (cervus elaphus) spermatozoa. METHOD OF STUDY: The semen samples collected from six mature red deer once a week were diluted with Tris-egg yolk-based extenders. The diluted semen samples were supplemented with glutathione (8 mmol L(-1) ) and or trehalose (5%, w/v), cryopreserved, thawed and then subjected to sperm quality parameter evaluation...
November 25, 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27885693/association-of-single-nucleotide-polymorphism-in-melatonin-receptor-1a-gene-with-egg-production-traits-in-yangzhou-geese
#18
M A Alsiddig, S G Yu, Z X Pan, H Widaa, T M Badri, J Chen, H L Liu
In the present study the melatonin receptor 1A gene (MTNR1A) was proposed to be a candidate gene for egg production in Yangzhou geese. A total of 210 goose blood samples were collected to investigate the association of the MTNR1A gene with the number of eggs produced. Using a direct sequencing method, a single nucleotide polymorphism (SNP; g.177G>C) was detected in the 5' regulatory region of the MTNR1A gene (Genbank ss1985399687). Two alleles (G and C) and three genotypes were identified. Association analysis results showed that the g...
November 24, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27884471/polybrominated-diphenyl-ethers-pbdes-and-hexa-brominated-biphenyls-hexa-bbs-in-fresh-foods-ingested-in-taiwan
#19
Jung-Wei Chang, Chung-Feng Hung, Ya-Chen Hsu, Yi-Ting Kao, Ching-Chang Lee
Polybrominated diphenyl ethers (PBDEs) and hexa-brominated biphenyls (Hexa-BBs) are bioaccumulative and aggregate in the food chain. Therefore, background monitoring and risk assessment for dietary intake are necessary. In present study, a systematic sampling method was first used to collect the high fat content foodstuff such as poultry, livestock, eggs, fish, other seafood, dairy products, and the infant foods and then foodstuff with high consumption in seven categories of 600 food samples. After integrating four years of background surveys of PBDE levels (2010-2013) and one year of that of Hexa-BBs (2013), the highest estimated daily intake (EDI) of PBDEs for Taiwanese food consumption was found in 0- to 3-year-olds (mean = 9...
January 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27884138/household-dietary-diversity-and-animal-source-food-consumption-in-ethiopia-evidence-from-the-2011-welfare-monitoring-survey
#20
Abdulhalik Workicho, Tefera Belachew, Garumma Tolu Feyissa, Beyene Wondafrash, Carl Lachat, Roosmarijn Verstraeten, Patrick Kolsteren
BACKGROUND: It is imperative to track dietary quality and progress in nutritional outcomes in a population to develop timely interventions. Dietary diversity is a commonly used proxy to assess dietary quality in low-income countries. This study identified predictors of household dietary diversity in Ethiopia and pattern of consumption of animal source food (ASF) among households. METHODS: Secondary data were analyzed from the 2011 Ethiopian Welfare Monitoring Survey (WMS)...
November 25, 2016: BMC Public Health
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