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https://www.readbyqxmd.com/read/29331832/investigations-on-a-cryopreservation-protocol-for-long-term-storage-of-psittacine-spermatozoa-using-cockatiel-semen-as-an-example
#1
H Schneider, D Fischer, K Failing, C Ehling, S Meinecke-Tillmann, A Wehrend, M Lierz
The aim of the present study was the establishment of an effective protocol for cryopreservation of psittacine semen. Therefore, pooled semen samples of 30 cockatiels (Nymphicus hollandicus) were diluted with modified Lake diluent (1:4), partitioned into four equal parts. Three portions were mixed with three cryoprotectants (dimethylacetamide, dimethyl sulfoxide, glycerol) in 4%, 8% and 12% final concentration, respectively, whereas the 4rth part served as control. Altogether, 96 incremental diluted semen samples were obtained for investigation...
December 16, 2017: Theriogenology
https://www.readbyqxmd.com/read/29329840/determination-of-colistin-in-animal-tissues-egg-milk-and-feed-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#2
Qin Fu, Xiaowei Li, Kangni Zheng, Yuebin Ke, Yingyu Wang, Lina Wang, Fugen Yu, Xi Xia
A confirmatory method for the determination of colistin in animal tissues, egg, milk, and feed was developed and validated. Colistin A and colistin B were extracted from samples with the mixture of 10% trichloroacetic acid-acetonitrile and isolated with mixed-mode weak cation exchange cartridge. Analytes were separated from matrix components using ultra-high performance liquid chromatography, and detected with electrospray ionization on a triple quadrupole mass spectrometer. Mean recoveries ranged from 78.0% to 115...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329626/applicability-of-flotac%C3%A2-technique-in-recovering-equine-strongyle-larvae-in-the-pasture-a-comparison-study
#3
Ingrid Carla do Nascimento Ramos, Rafael Antonio do Nascimento Ramos, Victor Fernando de Santana Lima, Alessio Giannelli, Irma Yaneth Torres López, Leucio Câmara Alves
The FLOTAC® technique represents a highly sensitive method for the isolation of oocysts, eggs, and larvae of parasites in faeces. This assay could be used for detecting free-living stages of nematodes in the pasture but no attempt has been assessed so far. Therefore, the performance of FLOTAC® technique for isolating infective larvae of nematodes in the environment was investigated and compared with the spontaneous sedimentation (SST) and centrifugal sedimentation (CST) techniques. The study was conducted in a horse farm located in northeastern Brazil, where the occurrence of strongyle larvae had been previously reported...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29325101/fauna-ecological-characteristics-and-checklist-of-the-mosquitoes-in-mazandaran-province-northern-iran
#4
Seyed Hassan Nikookar, Mahmoud Fazeli-Dinan, Shahyad Azari-Hamidian, Seyed Nouraddin Mousavi Nasab, Mohsen Aarabi, Seyyed Payman Ziapour, Ahmadali Enayati, Janet Hemingway
Mosquitoes are important vectors of human and animal diseases. This study updates current knowledge on fauna, dominance, and distribution of mosquitoes in Mazandaran Province, Northern Iran, to inform disease control effort. Larval collections, using standard dippers or droppers, and adult collections, using total catches, shelter pits, CDC light traps, and human landing catches, were performed monthly in 30 villages across 16 counties, from May to December 2014. Ovitraps, baited with hay infusion as oviposition attractants or stimulants for Aedes (Stegomyia) mosquitoes, were installed in each village and inspected weekly for eggs...
January 6, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29324834/experimental-transmission-of-west-nile-virus-and-rift-valley-fever-virus-by-culex-pipiens-from-lebanon
#5
Renée Zakhia, Laurence Mousson, Marie Vazeille, Nabil Haddad, Anna-Bella Failloux
West Nile virus (WNV) and Rift Valley fever virus (RVFV) are two emerging arboviruses transmitted by Culex pipiens species that includes two biotypes: pipiens and molestus. In Lebanon, human cases caused by WNV and RVFV have never been reported. However, the introduction of these viruses in the country is likely to occur through the migratory birds and animal trades. In this study, we evaluated the ability of Cx. pipiens, a predominant mosquito species in urban and rural regions in Lebanon, to transmit WNV and RVFV...
January 11, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29324085/hyperparasitism-of-an-avian-ectoparasitic-hippoboscid-fly-ornithomya-anchineuria-by-the-mite-myialges-cf-borealis-in-alberta-canada
#6
Cameron P Goater, Jayden Dyck, Heather Proctor, Kevin Floate
Hippoboscid flies (Diptera: Hippoboscidae) include species that are ectoparasites of birds in the Northern Hemisphere, but little is known regarding their taxonomy, parasites, avian host associations, or geographical distribution in North America. In late August of 2013 and 2014, we collected hippoboscid flies from live birds trapped in mist nets as part of a banding study in Cypress Hills Interprovincial Park in southeastern Alberta, Canada. A total of 113 birds comprising 9 species was examined in 2013. Of these, 18 individuals were infested with 1-3 Ornithomya anchineuria Speiser (n = 22 flies; prevalence = 15...
January 11, 2018: Journal of Parasitology
https://www.readbyqxmd.com/read/29321939/isolation-of-rickettsia-amblyommatis-in-huvec-line
#7
S Santibáñez, A Portillo, A M Palomar, J A Oteo
Rickettsia amblyommatis, formerly named Rickettsia amblyommii and 'Candidatus Rickettsia amblyommii' is an intracellular bacterium belonging to the spotted fever group Rickettsia. It is highly prevalent in Amblyomma americanum and in other Amblyomma spp. throughout the Western Hemisphere. R. amblyommatis has been cultivated in chicken fibroblast, primary embryonated chicken eggs, Vero cells and arthropod-derived cells. Because of the affinity of rickettsiae to invade vascular endothelial cells, we tried to isolate R...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29321913/egg-size-and-emergence-timing-affect-morphology-and-behavior-in-juvenile-chinook-salmon-oncorhynchus-tshawytscha
#8
Karen M Cogliati, Julia R Unrein, Heather A Stewart, Carl B Schreck, David L G Noakes
Variation in early life history traits often leads to differentially expressed morphological and behavioral phenotypes. We investigated whether variation in egg size and emergence timing influence subsequent morphology associated with migration timing in juvenile spring Chinook Salmon, Oncorhynchus tshawytscha. Based on evidence for a positive relationship between growth rate and migration timing, we predicted that fish from small eggs and fish that emerged earlier would have similar morphology to fall migrants, while fish from large eggs and individuals that emerged later would be more similar to older spring yearling migrants...
January 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29318631/early-life-gut-microbiome-and-egg-allergy
#9
Mina Fazlollahi, Yoojin Chun, Alexander Grishin, Robert A Wood, A Wesley Burks, Peter Dawson, Stacie M Jones, Donald Y M Leung, Hugh A Sampson, Scott H Sicherer, Supinda Bunyavanich
BACKGROUND: Gut microbiota may play a role in egg allergy. We sought to examine the association between early-life gut microbiota and egg allergy. METHODS: We studied 141 children with egg allergy and controls from the multi-center Consortium of Food Allergy Research study. At enrollment (age 3 to 16 months), fecal samples were collected and clinical evaluation, egg specific IgE measurement, and egg skin prick test were performed. Gut microbiome was profiled by 16S rRNA sequencing...
January 10, 2018: Allergy
https://www.readbyqxmd.com/read/29316983/urogenital-schistosomiasis-in-cabo-delgado-northern-mozambique-baseline-findings-from-the-score-study
#10
Anna E Phillips, Pedro H Gazzinelli-Guimarães, Herminio O Aurelio, Neerav Dhanani, Josefo Ferro, Rassul Nala, Arminder Deol, Alan Fenwick
BACKGROUND: The results presented here are part of a five-year cluster-randomised intervention trial that was implemented to understand how best to gain and sustain control of schistosomiasis through different preventive chemotherapy strategies. This paper presents baseline data that were collected in ten districts of Cabo Delgado province, northern Mozambique, before treatment. METHODS: A cross-sectional study of 19,039 individuals was sampled from 144 villages from May to September 2011...
January 10, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29315919/prevalence-and-longitudinal-trends-of-food-allergy-during-childhood-and-adolescence-results-of-the-isle-of-wight-birth-cohort-study
#11
D Venkataraman, M Erlewyn-Lajeunesse, R J Kurukulaaratchy, S Potter, G Roberts, S Matthews, S Hasan Arshad
BACKGROUND: The prevalence and time trends of food allergy change during childhood depending on the age of the child and the type of food. OBJECTIVE: To study prevalence and longitudinal trends in food allergy from birth to 18 years in an unselected birth cohort in the Isle of Wight. METHOD: Information on food allergy was collected at ages 1, 2, 4, 10 and 18 years from the Isle of Wight Birth Cohort (n = 1456). Skin prick testing (SPT) was performed at the age of 1 and 2 years in symptomatic children...
January 8, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29313753/compared-to-an-oatmeal-breakfast-two-eggs-day-increased-plasma-carotenoids-and-choline-without-increasing-trimethyl-amine-n-oxide-concentrations
#12
Amanda Missimer, Maria Luz Fernandez, Diana M DiMarco, Gregory H Norris, Christopher N Blesso, Ana Gabriela Murillo, Marcela Vergara-Jimenez, Bruno S Lemos, Isabel Medina-Vera, Olga V Malysheva, Marie A Caudill
BACKGROUND: Habitual consumption of eggs has been hypothesized to positively modify biomarkers of cardiovascular disease risk through proposed antioxidant properties. OBJECTIVES: To examine this relationship, 50 young, healthy men and women were enrolled into a randomized crossover clinical intervention. METHODS: Participants consumed either 2 eggs per day or one packet of oatmeal a day for 4 weeks, followed by a 3-week wash-out and crossed over to the alternate breakfast...
January 9, 2018: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/29307631/physicochemical-properties-of-anti-vibrio-harveyi-egg-yolk-antibody-igy-and-its-immunological-influence-in-indian-white-shrimp-fenneropenaeus-indicus
#13
Thankamani Kumaran, Eswaramoorthy Thirumalaikumar, Chinnadurai Lelin, Pandi Palanikumar, Mariavincent Michaelbabu, Thavasimuthu Citarasu
Edible antibodies specific to host pathogens is an attractive approach to establish protective immunity, especially against gastrointestinal pathogens both in humans and animals. The edible antibody of anti-Vibrio harveyi IgY (anti-V. h IgY) was produced by antigen mixed with immunoadjuvant Asparagus racemosus and Glycine max. Hens were immunized and eggs were collected five weeks after the immunization. Anti-V. harveyi IgY stability in different digestive enzymes such as trypsin and chymotrypsin were evaluated to determine its ability to withstand in the gastrointestinal tract of F...
January 4, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29290303/serological-evidence-for-eight-globally-important-poultry-viruses-in-trinidad-tobago
#14
Arianne Brown Jordan, Jamie Sookhoo, Lemar Blake, Paul Crooks, Zul Mohammed, Judy Molawatti-Bisnath, Christine V F Carrington, Christopher A L Oura
Viruses affecting poultry cause significant levels of disease leading to severe economic losses among poultry farmers worldwide. The Americas region continues to be vulnerable to the spread of poultry viruses across the continents and Caribbean island chains. In Trinidad and Tobago (T&T) there is limited information on the viruses circulating in poultry. Many flock are vulnerable to infection and there are occasional outbreaks of disease resulting in high levels of morbidity and mortality. This study aims to identify important viruses of poultry circulating in T&T through a broad-based surveillance approach...
January 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29289775/meltwater-from-snow-contaminated-by-oil-sands-emissions-is-toxic-to-larval-fish-but-not-spring-river-water
#15
J L Parrott, J R Marentette, L M Hewitt, M E McMaster, P L Gillis, W P Norwood, J L Kirk, K M Peru, J V Headley, Z Wang, C Yang, R A Frank
To assess the toxicity of winter-time atmospheric deposition in the oil sands mining area of Northern Alberta, embryo-larval fathead minnow (Pimephales promelas) were exposed to snowmelt samples. Snow was collected in 2011-2014 near (<7km) oil sands open pit mining operations in the Athabasca River watershed and at sites far from (>25km) oil sands mining. Snow was shipped frozen back to the laboratory, melted, and amended with essential ions prior to testing. Fertilized fathead minnow eggs were exposed (<24h post-fertilization to 7-16days post-hatch) to a range of 25%-100% snowmelt...
December 28, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29287895/a-two-step-dilution-tris-egg-yolk-extender-containing-equex-stm-significantly-improves-sperm-cryopreservation-in-the-african-wild-dog-lycaon-pictus
#16
Femke Van den Berghe, Monique Christina Johanna Paris, Michael Brent Briggs, Wenche Kristin Farstad, Damien Boyd Bertrand Paul Paris
Conservation management of endangered African wild dogs (AWD; Lycaon pictus) can benefit greatly from development of sperm freezing and artificial insemination. Previous freezing attempts yielded nearly 0% motile sperm within 2 h of thawing. In this study, two canine freezing protocols were tested: Protocol 1: a one-step dilution in TRIS-20% egg yolk containing 8% glycerol; and Protocol 2: a two-step dilution in TRIS-20% egg yolk containing a final extender concentration of 5% glycerol and 0.5% Equex STM, coupled with a TRIS-citrate-fructose thawing solution...
December 26, 2017: Cryobiology
https://www.readbyqxmd.com/read/29285561/latitudinal-variation-in-responses-of-a-forest-herbivore-and-its-egg-parasitoids-to-experimental-warming
#17
Mariana Abarca, John T Lill, Pablo Frank-Bolton
Disrupted biotic interactions are a predicted consequence of anthropogenic climate change when interactants differ in the magnitude or direction of phenological responses. Here, we examined the responses to artificial warming of northern, southern and central populations of the eastern tent caterpillar and its hymenopteran egg parasitoids. We subjected egg masses from each region to the typical conditions they experience in their source locality or to a warmer temperature regime, to quantify the effects of simulated warming on their relative phenology, survival and neonate starvation endurance...
December 28, 2017: Oecologia
https://www.readbyqxmd.com/read/29281642/transmission-of-rift-valley-fever-virus-from-european-breed-lambs-to-culex-pipiens-mosquitoes
#18
Rianka P M Vloet, Chantal B F Vogels, Constantianus J M Koenraadt, Gorben P Pijlman, Martin Eiden, Jose L Gonzales, Lucien J M van Keulen, Paul J Wichgers Schreur, Jeroen Kortekaas
BACKGROUND: Rift Valley fever virus (RVFV) is a mosquito-borne bunyavirus of the genus Phlebovirus that is highly pathogenic to ruminants and humans. The disease is currently confined to Africa and the Arabian Peninsula, but globalization and climate change may facilitate introductions of the virus into currently unaffected areas via infected animals or mosquitoes. The consequences of such an introduction will depend on environmental factors, the availability of susceptible ruminants and the capacity of local mosquitoes to transmit the virus...
December 27, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29281089/seasonal-reproductive-biology-of-drosophila-suzukii-diptera-drosophilidae-in-temperate-climates
#19
Alberto Grassi, Angela Gottardello, Daniel T Dalton, Gabriella Tait, Dalila Rendon, Claudio Ioriatti, David Gibeaut, M Valerio Rossi Stacconi, Vaughn M Walton
Drosophila suzukii (Matsumura; Diptera: Drosophilidae) is a key pest of sweet cherry and small fruits worldwide. The present studies were designed to describe the reproductive physiology in both sexes, through dissections of their reproductive organs. We extensively dissected female D. suzukii throughout the season from 2013 to 2016 and classified the reproductive status flies based on five recognizable ovarian maturation stages: 1) no ovaries; 2) unripe ovaries 3) ripening eggs in ovarioles; 4) mature eggs in ovarioles; and 5) mature eggs in the abdomen...
December 21, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/29279092/weight-gain-and-resistance-to-gastrointestinal-nematode-infections-in-two-genetically-diverse-groups-of-cattle
#20
Johan Höglund, Anna Hessle, Konstantinos Zaralis, Katarina Arvidsson-Segerkvist, Spiridoula Athanasiadou
Body weight gain (BWG) and gastrointestinal nematode challenge (GIN) were investigated in two genetically diverse groups of cattle. Thirty-two dairy calves (D=Swedish Red/Holstein) and 31 dairy×beef crosses (C=Swedish Red/Holstein×Charolais) pairwise matched by dam breed and birth dates, were monitored for ≈20 weeks on a pasture grazed by cattle in the previous year. At turn-out, animals (between 6 and 12 months age) from each genotype were either infected with 5000 third stage (L3) Ostertagia ostertagi (50%) and Cooperia oncophora (50%) larvae (H, high-exposure); or treated monthly with 0...
January 15, 2018: Veterinary Parasitology
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