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https://www.readbyqxmd.com/read/27600040/molecular-epidemiology-and-genome-dynamics-of-new-delhi-metallo-beta-lactamase-ndm-producing-extraintestinal-pathogenic-e-coli-expec-strains-from-india
#1
Amit Ranjan, Sabiha Shaik, Agnismita Mondal, Nishant Nandanwar, Arif Hussain, Torsten Semmler, Narender Kumar, Sumeet Tiwari, Savita Jadhav, Lothar H Wieler, Niyaz Ahmed
The global dissemination amidst increasing incidence of carbapenem-resistant, Gram-negative organisms has resulted in acute public health concerns. Here, we present a retrospective multicenter study on molecular characterization of metallo-beta-lactamase (MBL) producing clinical E. coli isolates recovered from extraintestinal infections in two hospitals in Pune, India. We screened a large sample size of 510 E. coli isolates for MBL production wherein we profiled their molecular determinants, antimicrobial resistance phenotypes, functional virulence properties, genomic features and transmission dynamics...
September 6, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27530831/ctx-m-15-in-combination-with-aac-6-ib-cr-is-the-most-prevalent-mechanism-of-resistance-both-in-escherichia-coli-and-klebsiella-pneumoniae-including-k-pneumoniae-st258-in-an-icu-in-uruguay
#2
Inés Bado, Claudia Gutiérrez, Virginia García-Fulgueiras, Nicolás F Cordeiro, Lucía Araújo Pirez, Verónica Seija, Cristina Bazet, Gloria Rieppi, Rafael Vignoli
The objectives of this study were (i) to determine the extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EcKp) clones circulating in an intensive care unit (ICU) in Uruguay between August 2010 and July 2011, (ii) to characterise the ESBL and plasmid-mediated quinolone resistance (PMQR) genes of the studied isolates and (iii) to determine the virulotype of the clinical isolates. Clinical and gut-colonising ESBL-EcKp from ICU patients were studied. Bacterial identification and antibiotic susceptibility determination were performed using a VITEK(®)2 system...
September 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27482804/characteristics-of-ciprofloxacin-resistant-enterobacteriaceae-isolates-recovered-from-wastewater-of-an-algerian-hospital
#3
Lynda Anssour, Yamina Messai, Vanesa Estepa, Carmen Torres, Rabah Bakour
INTRODUCTION: Hospital effluents are a source of environmental pollution by drugs, antibiotic-resistant bacteria, and resistance genes. Quinolones, particularly ciprofloxacin, are commonly detected in these effluents, contributing to the emergence of antimicrobial resistance. The objective of this study was to characterize ciprofloxacin-resistant Enterobacteriaceae in hospital effluents. METHODOLOGY: Isolates were selected on Tergitol-7 agar supplemented with ciprofloxacin and genotyped by ERIC-PCR...
2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/27462301/occurrence-of-oxa-48-carbapenemase-and-other-%C3%AE-lactamase-genes-in-esbl-producing-multidrug-resistant-escherichia-coli-from-dogs-and-cats-in-the-united-states-2009-2013
#4
Xiaoqiang Liu, Kamoltip Thungrat, Dawn M Boothe
OBJECTIVE: The aim of this study was to explore the occurrence and molecular characterization of extended-spectrum β-lactamases (ESBL), plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemases among ESBL-producing multidrug resistant (MDR) Escherichia coli from dogs and cats in the United States. METHODS: Of 2443 E.coli isolated from dogs and cats collected between August 2009 and January 2013, 68 isolates were confirmed as ESBL-producing MDR ones. PCR and sequencing were performed to identify β-lactamases and plasmid-mediated quinolone resistance (PMQR) genes, and shed light on the virulence gene profiles, phylogenetic groups and ST types...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27363476/characteristics-of-the-molecular-epidemiology-of-ctx-m-producing-escherichia-coli-isolated-from-a-tertiary-hospital-in-daejeon-korea
#5
Semi Kim, Ji Youn Sung, Hye Hyun Cho, Kye Chul Kwon, Sun Hoe Koo
The aims of this study were to characterize the molecular epidemiological profiles of CTX-M-producing uropathogenic Escherichia coli isolates from a tertiary hospital in Daejeon, Korea, and to investigate the genetic diversity and compare the prevalence of sequence types (STs) in different areas. Extended spectrum β-lactamase-producing E. coli strains isolated from urine were analyzed for CTX-M, integrons, and insertion sequence common regions (ISCRs) by PCR and sequencing. Multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), phylogenetic analysis, and rep-PCR were also used for molecular typing of the isolates...
September 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27220012/high-prevalence-of-extended-spectrum-and-plasmidic-ampc-beta-lactamase-producing-escherichia-coli-from-poultry-in-tunisia
#6
Elaa Maamar, Samia Hammami, Carla Andrea Alonso, Nouha Dakhli, Mohamed Salah Abbassi, Sana Ferjani, Zaineb Hamzaoui, Mabrouka Saidani, Carmen Torres, Ilhem Boutiba-Ben Boubaker
This study was conducted to detect extended spectrum beta-lactamases (ESBLs) and plasmidic AmpC beta-lactamase (pAmpC-BL)-producing Escherichia coli isolates in industrial poultry samples were collected from healthy chickens of the three farms. Samples were inoculated onto desoxycholate-lactose-agar plates supplemented with cefotaxime (2mg/L). E. coli was identified by biochemical and molecular methods and antibiotic susceptibility testing by the disk diffusion method. Genes encoding ESBLs and pAmpC-BL were detected by PCR and sequencing...
August 16, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27157499/detection-and-characterization-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-from-chicken-production-chains-in-nigeria
#7
Olufemi E Ojo, Stefan Schwarz, Geovana B Michael
A total of 405 Escherichia coli from the chicken production chains in Nigeria were investigated for ESBL-production and 4 isolates were identified as ESBL producers. They were characterized by XbaI-PFGE, multilocus sequence typing (MLST), phylotyping, sequencing of porin and regulatory genes and of the regulatory region of chromosomal ampC genes. Transformed ESBL gene-carrying plasmids were characterized by S1-nuclease, replicon typing, conjugation, digestion and PCRs for detection of the genetic environment of ESBL genes...
April 27, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27118773/characterization-of-an-outbreak-due-to-ctx-m-15-producing-klebsiella-pneumoniae-lacking-the-blaoxa-48-gene-belonging-to-clone-st405-in-a-neonatal-unit-in-southern-spain
#8
LETTER
Jesús Machuca, Lorena López-Cerero, Felipe Fernández-Cuenca, Irene Gracia-Ahufinger, Guillermo Ruiz-Carrascoso, Fernando Rodríguez-López, Álvaro Pascual
No abstract text is available yet for this article.
August 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/26871556/characteristics-of-extended-spectrum-%C3%AE-lactamase-esbl-and-pampc-beta-lactamase-producing-enterobacteriaceae-of-water-samples-in-tunisia
#9
Leila Ben Said, Ahlem Jouini, Carla Andrea Alonso, Naouel Klibi, Raoudha Dziri, Abdellatif Boudabous, Karim Ben Slama, Carmen Torres
The presence of extended-spectrum beta-lactamase and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae (ESBL-Eb and pAmpC-Eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in Tunisia. Twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained ESBL-Eb or pAmpC-Eb; one ESBL/pAmpC-Eb per positive sample was further characterized. Beta-lactamase genes detected were as follows: blaCTX-M-1 (10 Escherichia coli),blaCTX-M-15 (eight E...
April 15, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/26829536/extended-spectrum-%C3%AE-lactamases-transferable-quinolone-resistance-and-virulotyping-in-extra-intestinal-e-coli-in-uruguay
#10
Rafael Vignoli, Virginia García-Fulgueiras, Nicolás F Cordeiro, Inés Bado, Verónica Seija, Paula Aguerrebere, Gabriel Laguna, Lucía Araújo, Cristina Bazet, Gabriel Gutkind, José Chabalgoity
INTRODUCTION: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. METHODOLOGY: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates...
January 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/26824702/antimicrobial-resistance-and-molecular-epidemiology-of-escherichia-coli-causing-bloodstream-infections-in-three-hospitals-in-shanghai-china
#11
MULTICENTER STUDY
Su Wang, Sheng-Yuan Zhao, Shu-Zhen Xiao, Fei-Fei Gu, Qing-Zhong Liu, Jin Tang, Xiao-Kui Guo, Yu-Xing Ni, Li-Zhong Han
Escherichia coli (E. coli) is one of the most frequent and lethal causes of bloodstream infections (BSIs). We carried out a retrospective multicenter study on antimicrobial resistance and phylogenetic background of clinical E. coli isolates recovered from bloodstream in three hospitals in Shanghai. E. coli isolates causing BSIs were consecutively collected between Sept 2013 and Sept 2014. Ninety isolates randomly selected (30 from each hospital) were enrolled in the study. Antimicrobial susceptibility testing was performed by disk diffusion...
2016: PloS One
https://www.readbyqxmd.com/read/26769896/phylogeny-resistome-and-mobile-genetic-elements-of-emergent-oxa-48-and-oxa-245-klebsiella-pneumoniae-clones-circulating-in-spain
#12
María Pérez-Vázquez, Jesús Oteo, Silvia García-Cobos, Belén Aracil, Simon R Harris, Adriana Ortega, Dionisia Fontanals, Juan Manuel Hernández, Sonia Solís, José Campos, Gordon Dougan, Robert A Kingsley
OBJECTIVES: The global emergence of OXA-48-producing Klebsiella pneumoniae clones is a significant threat to public health. We used WGS and phylogenetic analysis of Spanish isolates to investigate the population structure of blaOXA-48-like-expressing K. pneumoniae ST11 and ST405 and to determine the distribution of resistance genes and plasmids encoding blaOXA-48-like carbapenemases. METHODS: SNPs identified in whole-genome sequences were used to reconstruct phylogenetic trees, identify resistance determinants and de novo assemble the genomes of 105 blaOXA-48-like-expressing K...
April 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/26552763/molecular-epidemiology-of-carbapenem-resistant-enterobacteriaceae-in-valle-d-aosta-region-italy-shows-the-emergence-of-kpc-2-producing-klebsiella-pneumoniae-clonal-complex-101-st101-and-st1789
#13
Mariateresa Del Franco, Laura Paone, Roberto Novati, Claudio G Giacomazzi, Maria Bagattini, Chiara Galotto, Pier Giorgio Montanera, Maria Triassi, Raffaele Zarrilli
BACKGROUND: The spread of carbapenem resistant Enterobacteriaceae (CRE) is an emerging clinical problem, of great relevance in Europe and worldwide. The aim of this study was the molecular epidemiology of CRE isolates in Valle d'Aosta region, Italy, and the mechanism of carbapenem resistance. RESULTS: Sixty consecutive CRE samples were isolated from 52 hospital inpatients and/or outpatients from November 2013 to August 2014. Genotyping of microbial isolates was done by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), carbapenemases were identified by PCR and sequencing...
2015: BMC Microbiology
https://www.readbyqxmd.com/read/26546855/comprehensive-clinical-and-epidemiological-assessment-of-colonisation-and-infection-due-to-carbapenemase-producing-enterobacteriaceae-in-spain
#14
MULTICENTER STUDY
Zaira R Palacios-Baena, Jesús Oteo, Carmen Conejo, M Nieves Larrosa, Germán Bou, Marta Fernández-Martínez, Juan José González-López, Vicente Pintado, Luis Martínez-Martínez, María Merino, Virginia Pomar, Marta Mora-Rillo, María Alba Rivera, Antonio Oliver, Guillermo Ruiz-Carrascoso, Patricia Ruiz-Garbajosa, Laura Zamorano, Verónica Bautista, Adriana Ortega, Isabel Morales, Álvaro Pascual, José Campos, Jesús Rodríguez-Baño
BACKGROUND: Most available information on carbapenemase-producing Enterobacteriaceae (CPE) is usually associated with specific types of infection or patient or with descriptions of outbreaks. The aim of this study was to comprehensively analyse the clinical epidemiology, clinical features and outcomes of colonisation and infections due to CPE in Spain. METHODS: A multicentre prospective cohort study was carried out in 34 Spanish hospitals from February to May 2013...
February 2016: Journal of Infection
https://www.readbyqxmd.com/read/26437344/distribution-of-virulence-factors-in-esbl-producing-escherichia-coli-isolated-from-the-environment-livestock-food-and-humans
#15
Andrea Müller, Roger Stephan, Magdalena Nüesch-Inderbinen
In this study, extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli isolates recovered from the following sources were characterized with regard to the occurrence and distribution of uropathogenic and enteric pathogenic virulence factors: surface waters (rivers and lakes, n=60), the intestines of freshwater fish (n=33), fresh vegetables (n=26), retail poultry meat (n=13) and the fecal samples of livestock (n=28), healthy humans (n=34) and primary care patients (n=13). Among the 207 isolates, 82% tested positive by PCR for one or more of the virulence factors (VF) that predict uropathogenicity, TraT, fyuA, chuA, PAI, yfcv or vat...
January 15, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/26183183/antibiotic-resistant-st38-st131-and-st405-strains-are-the-leading-uropathogenic-escherichia-coli-clones-in-riyadh-saudi-arabia
#16
Majed F Alghoribi, Tarek M Gibreel, Garry Farnham, Sameera M Al Johani, Hanan H Balkhy, Mathew Upton
OBJECTIVES: We investigated the molecular epidemiology of uropathogenic Escherichia coli (UPEC) from a tertiary care hospital in Riyadh, Saudi Arabia, revealing, for the first time, the population structure of UPEC in the region. METHODS: A total of 202 UPEC isolates were recovered from hospital and community patients with urinary tract infection in December 2012 and January 2013. Strains were characterized by MLST, antibiotic susceptibility determination and virulence gene detection...
October 2015: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/26098354/characterization-of-esbl-and-ampc-producing-enterobacteriaceae-from-diseased-companion-animals-in-europe
#17
Pierre Bogaerts, Te-Din Huang, Warda Bouchahrouf, Caroline Bauraing, Catherine Berhin, Farid El Garch, Youri Glupczynski
The study aimed to characterize beta-lactam resistance mechanisms of Enterobacteriaceae isolates recovered from diseased dogs and cats between 2008 and 2010 in a European surveillance program (ComPath I) for the antibiotic susceptibility of bacterial pathogens. A total of 608 non-duplicated Enterobacteriaceae isolates were obtained prior antibiotic treatment from diseased dogs (n=464) and cats (n=144). Among the 608 Enterobacteriaceae isolates, 22 presented a minimal inhibitory concentration against cefotaxime above EUCAST breakpoints of susceptibility...
December 2015: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/26030904/multidrug-resistant-and-extended-spectrum-beta-lactamase-producing-escherichia-coli-in-dutch-surface-water-and-wastewater
#18
Hetty Blaak, Gretta Lynch, Ronald Italiaander, Raditijo A Hamidjaja, Franciska M Schets, Ana Maria de Roda Husman
OBJECTIVE: The goal of the current study was to gain insight into the prevalence and concentrations of antimicrobial resistant (AMR) Escherichia coli in Dutch surface water, and to explore the role of wastewater as AMR contamination source. METHODS: The prevalence of AMR E. coli was determined in 113 surface water samples obtained from 30 different water bodies, and in 33 wastewater samples obtained at five health care institutions (HCIs), seven municipal wastewater treatment plants (mWWTPs), and an airport WWTP...
2015: PloS One
https://www.readbyqxmd.com/read/25969564/gut-colonization-of-healthy-children-and-their-mothers-with-pathogenic-ciprofloxacin-resistant-escherichia-coli
#19
Emily A Gurnee, I Malick Ndao, James R Johnson, Brian D Johnston, Mark D Gonzalez, Carey-Ann D Burnham, Carla M Hall-Moore, Jessica E McGhee, Alexander Mellmann, Barbara B Warner, Phillip I Tarr
BACKGROUND: The reservoir of pathogenic ciprofloxacin-resistant Escherichia coli remains unknown. METHODS: We conducted a prospective cohort study of 80 healthy twins and their mothers to determine the frequency of excretion of ciprofloxacin-resistant, potentially pathogenic E. coli. Stool specimens were cultured selectively for ciprofloxacin-resistant gram-negative bacteria. Isolates were categorized on the basis of additional resistance and virulence profiles...
December 15, 2015: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/25824224/prospective-multicenter-study-of-carbapenemase-producing-enterobacteriaceae-from-83-hospitals-in-spain-reveals-high-in-vitro-susceptibility-to-colistin-and-meropenem
#20
Jesús Oteo, Adriana Ortega, Rosa Bartolomé, Germán Bou, Carmen Conejo, Marta Fernández-Martínez, Juan José González-López, Laura Martínez-García, Luis Martínez-Martínez, María Merino, Elisenda Miró, Marta Mora, Ferran Navarro, Antonio Oliver, Álvaro Pascual, Jesús Rodríguez-Baño, Guillermo Ruiz-Carrascoso, Patricia Ruiz-Garbajosa, Laura Zamorano, Verónica Bautista, María Pérez-Vázquez, José Campos
The aim of this study was to determine the impact of carbapenemase-producing Enterobacteriaceae (CPE) in Spain in 2013 by describing the prevalence, dissemination, and geographic distribution of CPE clones, and their population structure and antibiotic susceptibility. From February 2013 to May 2013, 83 hospitals (about 40,000 hospital beds) prospectively collected nonduplicate Enterobacteriaceae using the screening cutoff recommended by EUCAST. Carbapenemase characterization was performed by phenotypic methods and confirmed by PCR and sequencing...
2015: Antimicrobial Agents and Chemotherapy
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