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Ivana Jamborova, Monika Dolejska, Ludek Zurek, Andrea K Townsend, Anne B Clark, Julie C Ellis, Ivo Papousek, Alois Cizek, Ivan Literak
American crow (Corvus brachyrhynchos) feces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 fecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality. Transferability of resistance genes was studied using conjugation and transformation bioassays...
March 9, 2017: Environmental Microbiology
Sima Tokajian, Tamara Salloum, Jonathan A Eisen, Guillaume Jospin, Anna Farra, Jacques E Mokhbat, David A Coil
AIM: Extended-spectrum β-lactamase-producing (ESBL) Escherichia coli are a public threat worldwide. This study aimed at analyzing the genomic and functional attributes of nine ESBLs taken from rectal swabs. MATERIALS & METHODS: Samples were isolated from patients admitted for gastrointestinal and urological procedures at the University Medical Center-Rizk Hospital (UMCRH) in Lebanon. Illumina paired-end libraries were prepared and sequenced. RESULTS: The isolates were distributed into five lineages: ST131, ST648, ST405, ST73 and ST38, and harbored bla OXA-1, bla TEM-1B, bla TEM-1C and aac(6')Ib-cr...
March 2017: Future Microbiology
Carla Andrea Alonso, Myriam Zarazaga, Rym Ben Sallem, Ahlem Jouini, Karim Ben Slama, Carmen Torres
In the last years different surveillances have been published in Africa, especially in Northern countries, regarding antimicrobial resistance among husbandry animals. Information is still scarce, but the available data shows a worrying picture. Although the highest resistance rates have been described against tetracycline, penicillins and sulphonamides, prevalence of plasmid mediated quinolone resistance genes and extended-spectrum β-lactamase (ESBL) are being increasingly reported. Among ESBLs, the CTX-M-1 group was dominant in most African surveys, being CTX-M-15 the main variant both in animals and humans, except in Tunisia where CTX-M-1 was more frequently detected among E...
February 16, 2017: Letters in Applied Microbiology
Zengmin Miao, Song Li, Lei Wang, Wengang Song, Yufa Zhou
This study aimed to investigate antimicrobial resistance and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) isolated from outpatients in town hospitals of Shandong province, China. Antimicrobial susceptibility of ESBL-producing E. coli was tested using the disk diffusion and resistance genes encoding for β-lactamases (blaTEM, blaCTXM, and blaSHV) were detected by polymerase chain reaction (PCR). Multilocus sequence typing (ST) of ESBL-producing E. coli was analyzed in this study...
2017: Frontiers in Microbiology
Lydia Yaici, Marisa Haenni, Véronique Métayer, Estelle Saras, Ferielle Mesbah Zekar, Meriem Ayad, Abdelaziz Touati, Jean-Yves Madec
The spread of Extended-Spectrum β-Lactamases (ESBLs) or AmpC β-Lactamases (AmpC) encoding genes in healthy human populations is of major concern. The role of the food chain has been questioned since numerous studies reported surface contamination of retail meat or crude vegetables with ESBL/AmpC-producing Enterobacteriaceae (ESBL/AmpC-E). Nonetheless, these food products are intended to be cooked or washed before consumption so that the risk of human transfer might be low. Here, the presence of ESBL/AmpC-E was investigated in ready-to-eat (RTE) sandwiches purchased in the street in the city of Bejaia, Algeria...
March 20, 2017: International Journal of Food Microbiology
Taous Bachiri, Sofiane Bakour, Rabia Ladjouzi, Leangapichart Thongpan, Jean Marc Rolain, Abdelaziz Touati
OBJECTIVES: The present study aimed to screen for the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in wild boars and Barbary macaques in Béjaïa and Jijel, Algeria. METHODS: A total of 216 faecal samples collected between September 2014 and August 2015 were cultured on MacConkey agar supplemented with 1μg/mL ceftazidime. Isolates were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS)...
December 10, 2016: Journal of Global Antimicrobial Resistance
Amit Ranjan, Sabiha Shaik, Agnismita Mondal, Nishant Nandanwar, Arif Hussain, Torsten Semmler, Narender Kumar, Sumeet K Tiwari, Savita Jadhav, Lothar H Wieler, Niyaz Ahmed
The global dissemination and increasing incidence of carbapenem-resistant, Gram-negative organisms have resulted in acute public health concerns. Here, we present a retrospective multicenter study on molecular characterization of metallo-β-lactamase (MBL)-producing clinical Escherichia coli isolates recovered from extraintestinal infections in two hospitals in Pune, India. We screened a large sample size of 510 E. coli isolates for MBL production wherein we profiled their molecular determinants, antimicrobial resistance phenotypes, functional virulence properties, genomic features, and transmission dynamics...
November 2016: Antimicrobial Agents and Chemotherapy
Inés Bado, Claudia Gutiérrez, Virginia García-Fulgueiras, Nicolás F Cordeiro, Lucía Araújo Pirez, Verónica Seija, Cristina Bazet, Gloria Rieppi, Rafael Vignoli
The objectives of this study were (i) to determine the extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EcKp) clones circulating in an intensive care unit (ICU) in Uruguay between August 2010 and July 2011, (ii) to characterise the ESBL and plasmid-mediated quinolone resistance (PMQR) genes of the studied isolates and (iii) to determine the virulotype of the clinical isolates. Clinical and gut-colonising ESBL-EcKp from ICU patients were studied. Bacterial identification and antibiotic susceptibility determination were performed using a VITEK(®)2 system...
September 2016: Journal of Global Antimicrobial Resistance
Lynda Anssour, Yamina Messai, Vanesa Estepa, Carmen Torres, Rabah Bakour
INTRODUCTION: Hospital effluents are a source of environmental pollution by drugs, antibiotic-resistant bacteria, and resistance genes. Quinolones, particularly ciprofloxacin, are commonly detected in these effluents, contributing to the emergence of antimicrobial resistance. The objective of this study was to characterize ciprofloxacin-resistant Enterobacteriaceae in hospital effluents. METHODOLOGY: Isolates were selected on Tergitol-7 agar supplemented with ciprofloxacin and genotyped by ERIC-PCR...
August 2, 2016: Journal of Infection in Developing Countries
Xiaoqiang Liu, Kamoltip Thungrat, Dawn M Boothe
OBJECTIVE: The aim of this study was to explore the occurrence and molecular characterization of extended-spectrum β-lactamases (ESBL), plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemases among ESBL-producing multidrug resistant (MDR) Escherichia coli from dogs and cats in the United States. METHODS: Of 2443 E.coli isolated from dogs and cats collected between August 2009 and January 2013, 68 isolates were confirmed as ESBL-producing MDR ones. PCR and sequencing were performed to identify β-lactamases and plasmid-mediated quinolone resistance (PMQR) genes, and shed light on the virulence gene profiles, phylogenetic groups and ST types...
2016: Frontiers in Microbiology
Semi Kim, Ji Youn Sung, Hye Hyun Cho, Kye Chul Kwon, Sun Hoe Koo
The aims of this study were to characterize the molecular epidemiological profiles of CTX-M-producing uropathogenic Escherichia coli isolates from a tertiary hospital in Daejeon, Korea, and to investigate the genetic diversity and compare the prevalence of sequence types (STs) in different areas. Extended spectrum β-lactamase-producing E. coli strains isolated from urine were analyzed for CTX-M, integrons, and insertion sequence common regions (ISCRs) by PCR and sequencing. Multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), phylogenetic analysis, and rep-PCR were also used for molecular typing of the isolates...
September 28, 2016: Journal of Microbiology and Biotechnology
Elaa Maamar, Samia Hammami, Carla Andrea Alonso, Nouha Dakhli, Mohamed Salah Abbassi, Sana Ferjani, Zaineb Hamzaoui, Mabrouka Saidani, Carmen Torres, Ilhem Boutiba-Ben Boubaker
This study was conducted to detect extended spectrum beta-lactamases (ESBLs) and plasmidic AmpC beta-lactamase (pAmpC-BL)-producing Escherichia coli isolates in industrial poultry samples were collected from healthy chickens of the three farms. Samples were inoculated onto desoxycholate-lactose-agar plates supplemented with cefotaxime (2mg/L). E. coli was identified by biochemical and molecular methods and antibiotic susceptibility testing by the disk diffusion method. Genes encoding ESBLs and pAmpC-BL were detected by PCR and sequencing...
August 16, 2016: International Journal of Food Microbiology
Olufemi E Ojo, Stefan Schwarz, Geovana B Michael
A total of 405 Escherichia coli from the chicken production chains in Nigeria were investigated for ESBL-production and 4 isolates were identified as ESBL producers. They were characterized by XbaI-PFGE, multilocus sequence typing (MLST), phylotyping, sequencing of porin and regulatory genes and of the regulatory region of chromosomal ampC genes. Transformed ESBL gene-carrying plasmids were characterized by S1-nuclease, replicon typing, conjugation, digestion and PCRs for detection of the genetic environment of ESBL genes...
October 15, 2016: Veterinary Microbiology
Jesús Machuca, Lorena López-Cerero, Felipe Fernández-Cuenca, Irene Gracia-Ahufinger, Guillermo Ruiz-Carrascoso, Fernando Rodríguez-López, Álvaro Pascual
No abstract text is available yet for this article.
August 2016: Journal of Antimicrobial Chemotherapy
Leila Ben Said, Ahlem Jouini, Carla Andrea Alonso, Naouel Klibi, Raoudha Dziri, Abdellatif Boudabous, Karim Ben Slama, Carmen Torres
The presence of extended-spectrum beta-lactamase and plasmid-mediated AmpC beta-lactamase producing Enterobacteriaceae (ESBL-Eb and pAmpC-Eb, respectively) was analyzed in 57 wastewater and 57 surface-water samples in Tunisia. Twenty-four of the 57 wastewater samples (42.1%) and one of the 57 surface-water samples (1.7%, a river that received effluents of a wastewater-treatment-plant) contained ESBL-Eb or pAmpC-Eb; one ESBL/pAmpC-Eb per positive sample was further characterized. Beta-lactamase genes detected were as follows: blaCTX-M-1 (10 Escherichia coli),blaCTX-M-15 (eight E...
April 15, 2016: Science of the Total Environment
Rafael Vignoli, Virginia García-Fulgueiras, Nicolás F Cordeiro, Inés Bado, Verónica Seija, Paula Aguerrebere, Gabriel Laguna, Lucía Araújo, Cristina Bazet, Gabriel Gutkind, José Chabalgoity
INTRODUCTION: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals. METHODOLOGY: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates...
January 31, 2016: Journal of Infection in Developing Countries
Su Wang, Sheng-Yuan Zhao, Shu-Zhen Xiao, Fei-Fei Gu, Qing-Zhong Liu, Jin Tang, Xiao-Kui Guo, Yu-Xing Ni, Li-Zhong Han
Escherichia coli (E. coli) is one of the most frequent and lethal causes of bloodstream infections (BSIs). We carried out a retrospective multicenter study on antimicrobial resistance and phylogenetic background of clinical E. coli isolates recovered from bloodstream in three hospitals in Shanghai. E. coli isolates causing BSIs were consecutively collected between Sept 2013 and Sept 2014. Ninety isolates randomly selected (30 from each hospital) were enrolled in the study. Antimicrobial susceptibility testing was performed by disk diffusion...
2016: PloS One
María Pérez-Vázquez, Jesús Oteo, Silvia García-Cobos, Belén Aracil, Simon R Harris, Adriana Ortega, Dionisia Fontanals, Juan Manuel Hernández, Sonia Solís, José Campos, Gordon Dougan, Robert A Kingsley
OBJECTIVES: The global emergence of OXA-48-producing Klebsiella pneumoniae clones is a significant threat to public health. We used WGS and phylogenetic analysis of Spanish isolates to investigate the population structure of blaOXA-48-like-expressing K. pneumoniae ST11 and ST405 and to determine the distribution of resistance genes and plasmids encoding blaOXA-48-like carbapenemases. METHODS: SNPs identified in whole-genome sequences were used to reconstruct phylogenetic trees, identify resistance determinants and de novo assemble the genomes of 105 blaOXA-48-like-expressing K...
April 2016: Journal of Antimicrobial Chemotherapy
Mariateresa Del Franco, Laura Paone, Roberto Novati, Claudio G Giacomazzi, Maria Bagattini, Chiara Galotto, Pier Giorgio Montanera, Maria Triassi, Raffaele Zarrilli
BACKGROUND: The spread of carbapenem resistant Enterobacteriaceae (CRE) is an emerging clinical problem, of great relevance in Europe and worldwide. The aim of this study was the molecular epidemiology of CRE isolates in Valle d'Aosta region, Italy, and the mechanism of carbapenem resistance. RESULTS: Sixty consecutive CRE samples were isolated from 52 hospital inpatients and/or outpatients from November 2013 to August 2014. Genotyping of microbial isolates was done by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST), carbapenemases were identified by PCR and sequencing...
2015: BMC Microbiology
Zaira R Palacios-Baena, Jesús Oteo, Carmen Conejo, M Nieves Larrosa, Germán Bou, Marta Fernández-Martínez, Juan José González-López, Vicente Pintado, Luis Martínez-Martínez, María Merino, Virginia Pomar, Marta Mora-Rillo, María Alba Rivera, Antonio Oliver, Guillermo Ruiz-Carrascoso, Patricia Ruiz-Garbajosa, Laura Zamorano, Verónica Bautista, Adriana Ortega, Isabel Morales, Álvaro Pascual, José Campos, Jesús Rodríguez-Baño
BACKGROUND: Most available information on carbapenemase-producing Enterobacteriaceae (CPE) is usually associated with specific types of infection or patient or with descriptions of outbreaks. The aim of this study was to comprehensively analyse the clinical epidemiology, clinical features and outcomes of colonisation and infections due to CPE in Spain. METHODS: A multicentre prospective cohort study was carried out in 34 Spanish hospitals from February to May 2013...
February 2016: Journal of Infection
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