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Christel Dagher, Tamara Salloum, Sahar Alousi, Harout Arabaghian, George F Araj, Sima Tokajian
The emergence of carbapenem resistant Escherichia coli represents a serious public health concern. This study investigated the resistome, virulence, plasmids content and clonality of 27 carbapenem resistant E. coli isolated from 27 hospitalized patients at the American University of Beirut Medical Center (AUBMC) in Lebanon between 2012 and 2016. Whole-genome sequencing (WGS) data were used to identify resistance determinants. Multilocus sequence typing (MLST), pulsed field gel electrophoresis (PFGE), phylogenetic grouping and PCR-based replicon typing (PBRT) were also performed...
2018: PloS One
Xiaoqiang Liu, Haixia Liu, Le Wang, Qian Peng, Yinqian Li, Hongchao Zhou, Qinfan Li
Objectives: The aim of the present study was to explore the prevalence and molecular characterization of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli collected from pig farms in Northwest China. Methods: Between May 2015 and June 2017, a total of 456 E. coli isolates were collected from fecal samples of healthy and diarrheal pigs in Northwest China to screen the ESBL producers. The β-lactamases, plasmid-mediated quinolone resistance (PMQR) genes and virulence genes among ESBL producers were corroborated by PCR and sequencing...
2018: Frontiers in Microbiology
Elena López-Camacho, José Ramón Paño-Pardo, Guillermo Ruiz-Carrascoso, Jan-Jaap Wesselink, Silvia Lusa-Bernal, Ricardo Ramos-Ruiz, Susana Ovalle, Rosa Gómez-Gil, Verónica Pérez-Blanco, María Pérez-Vázquez, Paulino Gómez-Puertas, Jesús Mingorance
OBJECTIVES: The aim of this study was to investigate the structure of a broad and sustained hospital outbreak of OXA-48-producing Klebsiella pneumoniae (KpO48) belonging to sequence type 405 (ST405). METHODS: Whole-genome sequencing and comparison of ten ST405 KpO48 isolates obtained from clinical samples in our hospital was performed. Using stringent criteria, 36 single nucleotide polymorphisms (SNPs) were detected (range 0-21 in pairwise comparisons), and allele-specific PCR was used to call the SNPs among a larger set of isolates...
June 22, 2018: Journal of Global Antimicrobial Resistance
Jeremiah Seni, Giselle Peirano, Kenneth Okwong Okon, Yusuf Bara Jibrin, Alkali Mohammed, Stephen E Mshana, Rebekah DeVinney, Johann D D Pitout
Limited information is available regarding the population structure of extra-intestinal pathogenic Escherichia coli (ExPEC) in Africa. Antimicrobial resistance profiles, sequence types (STs) and fimH types were determined on 60 clinical ExPEC from Nigeria using a 7-single nucleotide polymorphism quantitative PCR and sequencing of certain genes. Different ST131 clades were identified with a multiplex PCR. The isolates were mostly obtained from urines (58.3%). Not-susceptibility rates were as follows: trimethoprim-sulfamethoxazole (98%), cefotaxime (68%), gentamicin (55%), ciprofloxacin (62%) and piperacillin-tazobactam (2%)...
September 2018: Diagnostic Microbiology and Infectious Disease
Christian Dohle, Gerit Korr, Michael Friedrichs, Volker Kullmann, Mei-Lin Tung, Martin Kaase, Holger Rüssmann, Dagmar Sissolak, Dirk Werber, Laura Becker, Stephan Fuchs, Yvonne Pfeifer, Torsten Semmler, Gudrun Widders, Tim Eckmanns, Guido Werner, Edith Zill, Sebastian Haller
BACKGROUND: In addition to acute care hospitals, rehabilitation centres are increasingly confronted with multi-resistant pathogens. Long durations of stay and intensive treatments impose special hygienic challenges. MATERIAL AND METHODS: We investigated an extended spectrum beta-lactamase-Klebsiella pneumoniae (ESBL-K. pneumoniae) outbreak in a neurorehabilitation centre. We defined confirmed cases as patients who stayed in the centre during the outbreak period and from whom ESBL-K...
May 2018: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
Marc Argente, Elisenda Miró, Carmina Martí, Ana Vilamala, Carles Alonso-Tarrés, Frederic Ballester, Ana Calderón, Carmen Gallés, Asunción Gasós, Beatriz Mirelis, Montserrat Morta, Montserrat Olsina, Goretti Sauca, Montserrat Sierra, Alba Rivera, Ferran Navarro
INTRODUCTION: To characterize OXA-48 carbapenemase-producing Klebsiella pneumoniae strains isolated after an increase in carbapenem resistance in Catalonia. METHODOLOGY: K. pneumoniae identification, antimicrobial susceptibility studies, the Modified Hodge Test method, amplification of antimicrobial resistance genes (against β-lactamases, quinolones and aminoglycosides), molecular typing (by PFGE and MLST), conjugation assays, plasmid characterization (PBRT-PCR and Southern blot), a description of mobile genetic elements and statistical analysis were done...
April 6, 2018: Enfermedades Infecciosas y Microbiología Clínica
Meiji Soe Aung, Nilar San, Win Win Maw, Thida San, Noriko Urushibara, Mitsuyo Kawaguchiya, Ayako Sumi, Nobumichi Kobayashi
The increasing trend of Escherichia coli producing extended-spectrum beta-lactamases (ESBLs) and carbapenemases is a global public health concern. In this study, prevalence and molecular characteristics of E. coli harboring ESBL and carbapenemase genes were investigated for 426 isolates derived from various clinical specimens in a teaching hospital in Yangon, Myanmar, for the 1-year period beginning January 2016. A total of 157 isolates (36.9%) were ESBL producers and harbored CTX-M-1 group genes (146 isolates; blaCTX-M-15 , blaCTX-M55 ) or CTX-M-9 group genes (11 isolates; blaCTX-M-14 , blaCTX-M-27 )...
March 22, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Laura Becker, Stephan Fuchs, Yvonne Pfeifer, Torsten Semmler, Tim Eckmanns, Gerit Korr, Dagmar Sissolak, Michael Friedrichs, Edith Zill, Mei-Lin Tung, Christian Dohle, Martin Kaase, Sören Gatermann, Holger Rüssmann, Matthias Steglich, Sebastian Haller, Guido Werner
Extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae pose an important threat of infection with increased morbidity and mortality, especially for immunocompromised patients. Here, we use the rise of multidrug-resistant K. pneumoniae in a German neurorehabilitation center from April 2015 to April 2016 to dissect the benefit of whole genome sequencing (WGS) for outbreak analyses. In total, 53 isolates were obtained from 52 patients and examined using WGS. Two independent analysis strategies (reference-based and -free) revealed the same distinct clusters of two CTX-M-15 producing K...
2018: Frontiers in Microbiology
Ana Carolina C Campos, Nathália L Andrade, Mithila Ferdous, Monika A Chlebowicz, Carla C Santos, Julio C D Correal, Jerome R Lo Ten Foe, Ana Cláudia P Rosa, Paulo V Damasco, Alex W Friedrich, John W A Rossen
Urinary tract infections (UTIs) are often caused by Escherichia coli . Their increasing resistance to broad-spectrum antibiotics challenges the treatment of UTIs. Whereas, E. coli ST131 is often multidrug resistant (MDR), ST69 remains susceptible to antibiotics such as cephalosporins. Both STs are commonly linked to community and nosocomial infections. E. coli phylogenetic groups B2 and D are associated with virulence and resistance profiles making them more pathogenic. Little is known about the population structure of E...
2018: Frontiers in Microbiology
V Pérez-Blanco, L Redondo-Bravo, G Ruíz-Carrascoso, J R Paño-Pardo, R Gómez-Gil, A Robustillo-Rodela, J García-Rodríguez, J Mingorance, R Herruzo
The main objective of our study was to describe the epidemiological and microbiological features of an oligoclonal hospital-wide outbreak caused by OXA-48-producing Enterobacteriaceae (OXA-48-PE). OXA-48 is a carbapenemase belonging to Ambler class D beta-lactamases, identified frequently in the Mediterranean and Southern European countries, and associated with several Enterobacteriaceae species. An outbreak of OXA-48-PE with a complex epidemic pattern was detected in January 2011. Initial control measures included contact precautions and the reinforcement of infection control practices, but despite all efforts made, the epidemiological situation hardly changed and new measures were implemented during 2013...
April 2018: Epidemiology and Infection
Xiaoxia Zhang, Yu Feng, Weilong Zhou, Alan McNally, Zhiyong Zong
Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying bla NDM-4, an uncommon bla NDM variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to bla NDM-4 , the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene bla CTX-M-15 ...
January 10, 2018: Scientific Reports
Taous Bachiri, Rym Lalaoui, Sofiane Bakour, Meriem Allouache, Nadia Belkebla, Jean Marc Rolain, Abdelaziz Touati
BACKGROUND: The aim of the present study was to screen for the presence of mcr-1 gene in Enterobacteriaceae isolated from Barbary macaques (Macaca sylvanus) in Algeria. MATERIALS AND METHODS: From January to April 2016, a total of 86 fresh stool samples from Barbary macaques were collected in the Toudja forest (Bejaia, Algeria). After isolation, the isolates were identified by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. Antibiotic susceptibility was determined by disk diffusion method, and minimum inhibitory concentration (MIC) of colistin was determined by E-test...
September 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Sabiha Shaik, Amit Ranjan, Sumeet K Tiwari, Arif Hussain, Nishant Nandanwar, Narender Kumar, Savita Jadhav, Torsten Semmler, Ramani Baddam, Mohammed Aminul Islam, Munirul Alam, Lothar H Wieler, Haruo Watanabe, Niyaz Ahmed
Escherichia coli sequence type 131 (ST131), a pandemic clone responsible for the high incidence of extraintestinal pathogenic E. coli (ExPEC) infections, has been known widely for its contribution to the worldwide dissemination of multidrug resistance. Although other ExPEC-associated and extended-spectrum-β-lactamase (ESBL)-producing E. coli clones, such as ST38, ST405, and ST648 have been studied widely, no comparative genomic data with respect to other genotypes exist for ST131. In this study, comparative genomic analysis was performed for 99 ST131 E...
October 24, 2017: MBio
Karima Zenati, Farida Sahli, Vincent Garcia, Sofiane Bakour, Djellali Belhadi, Jean Marc Rolain, Abdelaziz Touati
PURPOSE: The aim of this study is to characterize the molecular support of antibiotic resistance in MDR Klebsiella pneumoniae recovered from inanimate surfaces between March 2012 to February 2014 in three teaching hospitals (Setif, Bejaia and Constantine) in Algeria. RESULTS: Forty-four K. pneumoniae producing ESBL were detected and blaCTX-M-15 and blaCTX-M-3 were detected respectively in 41 and 3 isolates. These K. pneumoniae isolates producing ESBL were also resistant to gentamicin (87%), tobramicin (87%), ciprofloxacin (66%) and ofloxacin (62%)...
September 2017: Journal of Global Antimicrobial Resistance
Yanping Luo, Rong Luo, Hong Ding, Xiu Ren, Haipeng Luo, Ying Zhang, Liyan Ye, Shenghui Cui
Limited studies have characterized multiple carbapenem-resistant Escherichia coli isolates and compared their genetic characteristics through whole-genome sequencing (WGS) because of the scarcity of these isolates. In this study, we determined the prevalence of carbapenem resistance in 2,993 E. coli isolates collected from three Chinese hospitals. The carbapenem-resistant isolates were further characterized by WGS, and the molecular epidemiological characters and resistance mechanisms were explored through the publicly available platforms from the Center for Genomic Epidemiology...
March 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Ivana Jamborova, Monika Dolejska, Ludek Zurek, Andrea K Townsend, Anne B Clark, Julie C Ellis, Ivo Papousek, Alois Cizek, Ivan Literak
American crow (Corvus brachyrhynchos) faeces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 faecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality. Transferability of resistance genes was studied using conjugation and transformation bioassays...
May 2017: Environmental Microbiology
Sima Tokajian, Tamara Salloum, Jonathan A Eisen, Guillaume Jospin, Anna Farra, Jacques E Mokhbat, David A Coil
AIM: Extended-spectrum β-lactamase-producing (ESBL) Escherichia coli are a public threat worldwide. This study aimed at analyzing the genomic and functional attributes of nine ESBLs taken from rectal swabs. MATERIALS & METHODS: Samples were isolated from patients admitted for gastrointestinal and urological procedures at the University Medical Center-Rizk Hospital (UMCRH) in Lebanon. Illumina paired-end libraries were prepared and sequenced. RESULTS: The isolates were distributed into five lineages: ST131, ST648, ST405, ST73 and ST38, and harbored bla OXA-1 , bla TEM-1B , bla TEM-1C and aac(6')Ib-cr...
March 2017: Future Microbiology
C A Alonso, M Zarazaga, R Ben Sallem, A Jouini, K Ben Slama, C Torres
In the last few years, different surveillances have been published in Africa, especially in northern countries, regarding antimicrobial resistance among husbandry animals. Information is still scarce, but the available data show a worrying picture. Although the highest resistance rates have been described against tetracycline, penicillins and sulphonamides, prevalence of plasmid-mediated quinolone resistance genes and extended spectrum β-lactamase (ESBL) are being increasingly reported. Among ESBLs, the CTX-M-1 group was dominant in most African surveys...
May 2017: Letters in Applied Microbiology
Zengmin Miao, Song Li, Lei Wang, Wengang Song, Yufa Zhou
This study aimed to investigate antimicrobial resistance and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) isolated from outpatients in town hospitals of Shandong province, China. Antimicrobial susceptibility of ESBL-producing E. coli was tested using the disk diffusion and resistance genes encoding for β-lactamases (blaTEM, blaCTXM, and blaSHV) were detected by polymerase chain reaction (PCR). Multilocus sequence typing (ST) of ESBL-producing E. coli was analyzed in this study...
2017: Frontiers in Microbiology
Lydia Yaici, Marisa Haenni, Véronique Métayer, Estelle Saras, Ferielle Mesbah Zekar, Meriem Ayad, Abdelaziz Touati, Jean-Yves Madec
The spread of Extended-Spectrum β-Lactamases (ESBLs) or AmpC β-Lactamases (AmpC) encoding genes in healthy human populations is of major concern. The role of the food chain has been questioned since numerous studies reported surface contamination of retail meat or crude vegetables with ESBL/AmpC-producing Enterobacteriaceae (ESBL/AmpC-E). Nonetheless, these food products are intended to be cooked or washed before consumption so that the risk of human transfer might be low. Here, the presence of ESBL/AmpC-E was investigated in ready-to-eat (RTE) sandwiches purchased in the street in the city of Bejaia, Algeria...
March 20, 2017: International Journal of Food Microbiology
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