Matthew E H White, Ludwig R Sinn, D Marc Jones, Joost de Folter, Simran Kaur Aulakh, Ziyue Wang, Helen R Flynn, Lynn Krüger, Pinkus Tober-Lau, Vadim Demichev, Florian Kurth, Michael Mülleder, Véronique Blanchard, Christoph B Messner, Markus Ralser
Protein glycosylation, a complex and heterogeneous post-translational modification that is frequently dysregulated in disease, has been difficult to analyse at scale. Here we report a data-independent acquisition technique for the large-scale mass-spectrometric quantification of glycopeptides in plasma samples. The technique, which we named 'OxoScan-MS', identifies oxonium ions as glycopeptide fragments and exploits a sliding-quadrupole dimension to generate comprehensive and untargeted oxonium ion maps of precursor masses assigned to fragment ions from non-enriched plasma samples...
July 20, 2023: Nature Biomedical Engineering