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https://www.readbyqxmd.com/read/28823035/armored-dna-in-recombinant-baculoviruses-as-controls-in-molecular-genetic-assays
#1
Andrea Freystetter, Christian Paar, Herbert Stekel, Jörg Berg
The widespread use of molecular PCR-based assays in analytical and clinical laboratories brings about the need for test-specific, stable, and reliable external controls (EC) as well as standards and internal amplification controls (IC), in order to arrive at consistent test results. In addition, there is also a growing need to produce and provide stable, well-characterized molecular controls for quality assurance programs. In this study, we describe a novel approach to generate armored double-stranded DNA controls, which are encapsulated in baculovirus (BV) particles of the species Autographa californica multiple nucleopolyhedrovirus...
August 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28822996/examining-bias-in-studies-of-statin-treatment-and-survival-in-patients-with-cancer
#2
Louise Emilsson, Xabier García-Albéniz, Roger W Logan, Ellen C Caniglia, Mette Kalager, Miguel A Hernán
Importance: Patients with cancer who use statins appear to have a substantially better survival than nonusers in observational studies. However, this inverse association between statin use and mortality may be due to selection bias and immortal-time bias. Objective: To emulate a randomized trial of statin therapy initiation that is free of selection bias and immortal-time bias. Design, Setting, and Participants: We used observational data on 17 372 patients with cancer from the Surveillance, Epidemiology, and End Results (SEER)-Medicare database (2007-2009) with complete follow-up until 2011...
August 20, 2017: JAMA Oncology
https://www.readbyqxmd.com/read/28822995/determination-of-norflurazon-concentration-in-wheat-leaves-using-a-modified-quechers-method
#3
Kamil F Trzebuniak, Beata Mysliwa-Kurdziel
The aim of this analytical study was to develop and validate an easy-to-use method for measuring the actual level of norflurazon that accumulates in leaves. We amended the QuEChERS method, i.e. Quick, Easy, Cheap, Effective, Rugged, and Safe, which is widely used for pesticide and herbicide analysis in food, and usually combined with HPLC-MS detection. We adapted this method for the detection of norflurazon in leaves or leaf fragments and proposed a useful modification using of HPLC-UV detection. Reproducible retention times of 3...
August 19, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28822982/evaluation-of-aortic-regurgitation-with-cardiac-magnetic-resonance-imaging-a-systematic-review
#4
REVIEW
James C Lee, Kelley R Branch, Christian Hamilton-Craig, Eric V Krieger
This review summaries the utility, application and data supporting use of cardiac magnetic resonance imaging (CMR) to evaluate and quantitate aortic regurgitation. We systematically searched Medline and PubMed for original research articles published since 2000 that provided data on the quantitation of aortic regurgitation by CMR and identified 11 articles for review. Direct aortic measurements using phase contrast allow quantitation of volumetric flow across the aortic valve and are highly reproducible and accurate compared with echocardiography...
August 19, 2017: Heart: Official Journal of the British Cardiac Society
https://www.readbyqxmd.com/read/28822875/a-consortium-driven-framework-to-guide-the-implementation-of-ich-m7-option-4-control-strategies
#5
Chris Barber, Vincent Antonucci, Jens-Christoph Baumann, Roland Brown, Elizabeth Covey-Crump, David Elder, Eric Elliott, Jared W Fennell, Fabrice Gallou, Nathan D Ide, Guido Jordine, Jeffrey M Kallemeyn, Dirk Lauwers, Adam R Looker, Lucie E Lovelle, Mark McLaughlin, Robert Molzahn, Martin Ott, Didier Schils, Rolf Schulte Oestrich, Neil Stevenson, Pere Talavera, Andrew Teasdale, Michael W Urquhart, David L Varie, Dennie Welch
The ICH M7 Option 4 control of (potentially) mutagenic impurities is based on the use of scientific principles in lieu of routine analytical testing. This approach can reduce the burden of analytical testing without compromising patient safety, provided a scientifically rigorous approach is taken which is backed up by sufficient theoretical and/or analytical data. This paper introduces a consortium-led initiative and offers a proposal on the supporting evidence that could be presented in regulatory submissions...
August 16, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28822801/microarray-gene-expression-study-in-fibroblast-and-lymphoblastoid-cell-lines-from-antipsychotic-na%C3%A3-ve-first-episode-schizophrenia-patients
#6
Patricia Gassó, Sergi Mas, Natalia Rodríguez, Daniel Boloc, Susana García-Cerro, Miquel Bernardo, Amalia Lafuente, Eduard Parellada
Schizophrenia (SZ) is a chronic psychiatric disorder whose onset of symptoms occurs in late adolescence and early adulthood. The etiology is complex and involves important gene-environment interactions. Microarray gene-expression studies on SZ have identified alterations in several biological processes. The heterogeneity in the results can be attributed to the use of different sample types and other important confounding factors including age, illness chronicity and antipsychotic exposure. The aim of the present microarray study was to analyze, for the first time to our knowledge, differences in gene expression profiles in 18 fibroblast (FCLs) and 14 lymphoblastoid cell lines (LCLs) from antipsychotic-naïve first-episode schizophrenia (FES) patients and healthy controls...
August 5, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28822794/lipidomics-informatics-for-life-science
#7
D Schwudke, A Shevchenko, N Hoffmann, R Ahrends
Lipidomics encompasses analytical approaches that aim to identify and quantify the complete set of lipids, defined as lipidome in a given cell, tissue or organism as well as their interactions with other molecules. The majority of lipidomics workflows is based on mass spectrometry and has been proven as a powerful tool in system biology in concert with other Omics disciplines. Unfortunately, bioinformatics infrastructures for this relatively young discipline are limited only to some specialists. Search engines, quantification algorithms, visualization tools and databases developed by the 'Lipidomics Informatics for Life-Science' (LIFS) partners will be restructured and standardized to provide broad access to these specialized bioinformatics pipelines...
August 16, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28822772/differentiation-of-candida-albicans-candida-glabrata-and-candida-krusei-by-ft-ir-and-chemometrics-by-chromagar%C3%A2-candida
#8
Denise Wohlmeister, Débora Renz Barreto Vianna, Virginia Etges Helfer, Luciane Noal Calil, Andréia Buffon, Alexandre Meneghello Fuentefria, Valeriano Antonio Corbellini, Diogo André Pilger
Pathogenic Candida species are detected in clinical infections. CHROMagar™ is a phenotypical method used to identify Candida species, although it has limitations, which indicates the need for more sensitive and specific techniques. Infrared Spectroscopy (FT-IR) is an analytical vibrational technique used to identify patterns of metabolic fingerprint of biological matrixes, particularly whole microbial cell systems as Candida sp. in association of classificatory chemometrics algorithms. On the other hand, Soft Independent Modeling by Class Analogy (SIMCA) is one of the typical algorithms still little employed in microbiological classification...
August 16, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28822313/a-selective-glucose-sensor-based-on-direct-oxidation-on-a-bimetal-catalyst-with-a-molecular-imprinted-polymer
#9
Seong Je Cho, Hui-Bog Noh, Mi-Sook Won, Chul-Ho Cho, Kwang Bok Kim, Yoon-Bo Shim
A selective nonenzymatic glucose sensor was developed based on the direct oxidation of glucose on hierarchical CuCo bimetal-coated with a glucose-imprinted polymer (GIP). Glucose was introduced into the GIP composed of Nafion and polyurethane along with aminophenyl boronic acid (APBA), which was formed on the bimetal electrode formed on a screen-printed electrode. The extraction of glucose from the GIP allowed for the selective permeation of glucose into the bimetal electrode surface for oxidation. The GIP-coated bimetal sensor probe was characterized using electrochemical and surface analytical methods...
August 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28822228/computerized-neurocognitive-interventions-in-the-context-of-the-brain-training-controversy
#10
Rebeca Isabel García-Betances, María Fernanda Cabrera-Umpiérrez, María T Arredondo
This article presents, in the form of an analytic narrative review, a complete picture of the state-of-the-art, challenges, and perspectives in the field of information and communication technology (ICT)-based neurocognitive interventions for older adults. The narrative particularly focuses on applications aimed at mild cognitive impairment and similar age-related cognitive deficits, which are analyzed in the context of the brain training controversy. Clarifying considerations are provided about the nature and present extent of the brain training debate, regarding the possible influence it has on the support received by research and development initiatives dealing with innovative computerized neurocognitive interventions...
August 19, 2017: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/28821937/natural-hazard-susceptibility-assessment-for-road-planning-using-spatial-multi-criteria-analysis
#11
Caroline S J Karlsson, Zahra Kalantari, Ulla Mörtberg, Bo Olofsson, Steve W Lyon
Inadequate infrastructural networks can be detrimental to society if transport between locations becomes hindered or delayed, especially due to natural hazards which are difficult to control. Thus determining natural hazard susceptible areas and incorporating them in the initial planning process, may reduce infrastructural damages in the long run. The objective of this study was to evaluate the usefulness of expert judgments for assessing natural hazard susceptibility through a spatial multi-criteria analysis approach using hydrological, geological, and land use factors...
August 18, 2017: Environmental Management
https://www.readbyqxmd.com/read/28821802/dispersive-regimes-of-the-dicke-model
#12
Diego Barberena, Lucas Lamata, Enrique Solano
We study two dispersive regimes of the Dicke model in the dynamics of N two-level atoms interacting with a bosonic mode for long interaction times. Firstly, we analyze the model for the regime in which the qubit frequencies are equal and smaller than the mode frequency, and for values of the coupling strength similar or larger than the mode frequency, namely, the deep strong coupling regime. Secondly, we address an interaction that is dependent on the photon number, where the coupling strength is comparable to the geometric mean of the qubit and mode frequencies...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821776/mineralization-and-preservation-of-an-extremotolerant-bacterium-isolated-from-an-early-mars-analog-environment
#13
F Gaboyer, C Le Milbeau, M Bohmeier, P Schwendner, P Vannier, K Beblo-Vranesevic, E Rabbow, F Foucher, P Gautret, R Guégan, A Richard, A Sauldubois, P Richmann, A K Perras, C Moissl-Eichinger, C S Cockell, P Rettberg, Marteinsson, E Monaghan, P Ehrenfreund, L Garcia-Descalzo, F Gomez, M Malki, R Amils, P Cabezas, N Walter, F Westall
The artificial mineralization of a polyresistant bacterial strain isolated from an acidic, oligotrophic lake was carried out to better understand microbial (i) early mineralization and (ii) potential for further fossilisation. Mineralization was conducted in mineral matrixes commonly found on Mars and Early-Earth, silica and gypsum, for 6 months. Samples were analyzed using microbiological (survival rates), morphological (electron microscopy), biochemical (GC-MS, Microarray immunoassay, Rock-Eval) and spectroscopic (EDX, FTIR, RAMAN spectroscopy) methods...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821614/multiple-circadian-transcriptional-elements-cooperatively-regulate-cell-autonomous-transcriptional-oscillation-of-period3-a-mammalian-clock-gene
#14
Ritsuko Matsumura, Makoto Akashi
Cell-autonomous oscillation in clock gene expression drives circadian rhythms. The development of comprehensive analytical techniques, such as bioinformatics and ChIP-sequencing, has enabled the genome-wide identification of potential circadian transcriptional elements that regulate the transcriptional oscillation of clock genes. However, detailed analyses using traditional biochemical and molecular-biological approaches, such as binding and reporter assays, are still necessary to determine whether these potential circadian transcriptional elements are actually functional and how significantly they contribute to driving transcriptional oscillation...
August 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28821603/a-simple-light-isotope-metabolic-labeling-slim-labeling-strategy-a-powerful-tool-to-address-the-dynamics-of-proteome-variations-in-vivo
#15
Thibaut Léger, Camille Garcia, Laetitia Collomb, Jean-Michel Camadro
Many quantitative proteomics strategies rely on in vivo metabolic incorporation of amino acids with modified stable isotope profiles into proteins. These methods give rise to multiple ions for each peptide, with possible distortion of the isotopolog distribution, making the overall analytical process complex. We validated an alternative strategy, simple light isotope metabolic labeling (SLIM-labeling), which alleviates many of these problems. SLIM-labeling is based on the in vivo reduction of the isotopic composition of proteins using metabolic precursors with a unique light isotope composition to label all amino acids...
August 18, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28821484/measurement-and-mathematical-characterization-of-cell-level-pharmacokinetics-of-antibody-drug-conjugates-a-case-study-with-trastuzumab-vc-mmae
#16
Aman P Singh, Dhaval K Shah
The main objective of this work was to understand and mathematically characterize the cellular disposition of a tool ADC, trastuzumab-vc-MMAE (T-vc-MMAE). Towards this goal, three different analytical methods were developed to measure the concentrations of different ADC related analytes in the media and cell lysate. An LC-MS/MS method was developed to quantify unconjugated drug (i.e. MMAE) concentrations, a forced deconjugation method was developed to quantify total drug concentrations, and an ELISA method was developed to quantify total antibody (i...
August 18, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28821459/clinical-multi-omics-strategies-for-the-effective-cancer-management
#17
REVIEW
Byong Chul Yoo, Kyung-Hee Kim, Sang Myung Woo, Jae Kyung Myung
Cancer is a global health issue as a multi-factorial complex disease, and early detection and novel therapeutic strategies are required for more effective cancer management. With the development of systemic analytical -omics strategies, the therapeutic approach and study of the molecular mechanisms of carcinogenesis and cancer progression have moved from hypothesis-driven targeted investigations to data-driven untargeted investigations focusing on the integrated diagnosis, treatment, and prevention of cancer in individual patients...
August 15, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28821348/viscous-dynamics-of-drops-and-bubbles-in-hele-shaw-cells-drainage-drag-friction-coalescence-and-bursting
#18
REVIEW
Ko Okumura
In this review article, we discuss recent studies on drops and bubbles in Hele-Shaw cells, focusing on how scaling laws exhibit crossovers from the three-dimensional counterparts and focusing on topics in which viscosity plays an important role. By virtue of progresses in analytical theory and high-speed imaging, dynamics of drops and bubbles have actively been studied with the aid of scaling arguments. However, compared with three-dimensional problems, studies on the corresponding problems in Hele-Shaw cells are still limited...
July 22, 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28821306/48243
#19
Sharon F Webb
A single-laboratory validation method for the simultaneous determination of nutrient and non-nutrient metals in commercial fertilizers was reviewed by the AOAC Expert Review Panel (ERP) and stakeholder panel for fertilizers at the 2017 midyear AOAC INTERNATIONAL meeting. In addition to using 15 of the original 30 fertilizer materials from the previous Method 2006.03 collaborative study, NIST Standard Certified Reference Material 695 and Magruder check sample 2009-06 were also incorporated as accuracy, recovery, and repeatability materials...
August 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28821304/determination-of-selenium-and-arsenic-ions-in-edible-mushroom-samples-by-novel-chloride-oxalic-acid-deep-eutectic-solvent-extraction-using-graphite-furnace-atomic-absorption-spectrometry
#20
Rizwan Ali Zounr, Mustafa Tuzen, Muhammad Yar Khuhawar
In present study, we proposed the application of a deep eutectic solvent (DES) made up of choline chloride (ChCl) and oxalic acid (Ox) for the dissolution of different edible mushroom samples for the determination of selenium (Se) and arsenic (As) ions. Therefore, an innovative, green, novel, and inexpensive method based on ChCl–Ox as the DES was developed for the determination of Se and As ions in mushroom species by graphite furnace-atomic absorption spectrometry. The important analytical parameters were also optimized...
August 18, 2017: Journal of AOAC International
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