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https://www.readbyqxmd.com/read/28549336/evaluation-of-a-new-random-access-hbv-dna-molecular-assay-the-veris-hbv-assay
#1
Slim Fourati, Dominique Challine, Jean-Dominique Poveda, Syria Laperche, Sandrine Rallier, Jean-Michel Pawlotsky, Stéphane Chevaliez
BACKGROUND: Detection and quantification of HBV DNA are essential to diagnose chronic HBV infection, monitor the virological response to treatment and the possible selection of resistant viruses in order to tailor therapy. The VERIS/MDx System HBV Assay is a random-access system that quantifies HBV DNA in clinical samples using unique single sample and reagent access during the workflow process without the need to reload other tests and delivers results within 1.2h following sampling...
May 17, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28549294/chiral-recognition-of-naproxen-enantiomers-based-on-fluorescence-quenching-of-bovine-serum-albumin-stabilized-gold-nanoclusters
#2
Marzieh Jafari, Javad Tashkhourian, Ghodratollah Absalan
A simple, fast and green method for chiral recognition of S- and R-naproxen has been introduced. The method was based on quenching of the fluorescence intensity of bovine serum albumin-stabilized gold nanoclusters in the presence of naproxen enantiomers. The quenching intensity in the presence of S-naproxen was higher than R-naproxen when phosphate buffer solution at pH7.0 was used. The chiral recognition occurred due to steric effect between bovine serum albumin conformation and naproxen enantiomers. Two linear determination range were established as 7...
May 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28549280/protocols-for-the-analytical-characterization-of-therapeutic-monoclonal-antibodies-i-non-denaturing-chromatographic-techniques
#3
Alexandre Goyon, Valentina D'Atri, Balazs Bobaly, Elsa Wagner-Rousset, Alain Beck, Szabolcs Fekete, Davy Guillarme
Size-, charge- and hydrophobicity-related variants of a biopharmaceutical product have to be deeply characterized for batch consistency and for the assessment of immunogenicity and safety effects. Size exclusion chromatography (SEC) and ion exchange chromatography (IEX) are considered as the gold standard for the analysis of high molecular weight species (HMWS) and charge-related variants, respectively. Hydrophobic interaction chromatography (HIC) has drawn renewed attention to monitor the small drug payload distribution in the cysteine-linked antibody-drug conjugates (ADC)...
May 11, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28549039/paired-comparison-of-pd-l1-expression-on-cytologic-and-histologic-specimens-from-malignancies-in-the-lung-assessed-with-pd-l1-ihc-28-8pharmdx-and-pd-l1-ihc-22c3pharmdx
#4
Birgit G Skov, Torsten Skov
BACKGROUND: Programmed cell death ligand-1 (PD-L1) expression is a predictive biomarker for anti-PD-1 immunotherapy in non-small cell lung cancer. Different immunohistochemistry (IHC) assays have been developed on histologic material with different cutoffs for positivity. More than one third of the patients are diagnosed on cytology alone. We hypothesized that cytologic cell block material is suitable for PD-L1 analysis. MATERIALS AND METHODS: Eighty-six paired samples of malignancies from the lung where cytologic cell block and histologic material were available from the same lesion were stained with PD-L1 IHC 28-8pharmDx and PD-L1 IHC 22C3pharmDx...
May 25, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28548845/a-new-analytical-framework-for-verification-of-biomarkers-of-exposure-to-chemicals-combining-human-biomonitoring-and-water-fingerprinting
#5
Luigi Lopardo, Andrew Cummins, Axel Rydevik, Barbara Kasprzyk-Hordern
Molecular epidemiology approaches in human biomonitoring are powerful tools that allow for verification of public exposure to chemical substances. Unfortunately, due to logistical difficulties and high cost, they tend to evaluate small study groups and as a result might not provide comprehensive large scale community-wide exposure data. Urban water fingerprinting provides a timely alternative to traditional approaches. It can revolutionise the human exposure studies as urban water represents collective community-wide exposure...
May 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28548572/analytical-validation-of-a-reference-laboratory-elisa-for-the-detection-of-feline-leukemia-virus-p27-antigen
#6
Jesse S Buch, Genevieve H Clark, Roberta Cahill, Brendon Thatcher, Peter Smith, Ramaswamy Chandrashekar, Christian M Leutenegger, Thomas P O'Connor, Melissa J Beall
Feline leukemia virus (FeLV) is an oncogenic retrovirus of cats. Immunoassays for the p27 core protein of FeLV aid in the detection of FeLV infections. Commercial microtiter-plate ELISAs have rapid protocols and visual result interpretation, limiting their usefulness in high-throughput situations. The purpose of our study was to validate the PetChek FeLV 15 ELISA, which is designed for the reference laboratory, and incorporates sequential, orthogonal screening and confirmatory protocols. A cutoff for the screening assay was established with 100% accuracy using 309 feline samples (244 negative, 65 positive) defined by the combined results of FeLV PCR and an independent reference p27 antigen ELISA...
May 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28548528/typical-relaxation-of-isolated-many-body-systems-which-do-not-thermalize
#7
Ben N Balz, Peter Reimann
We consider isolated many-body quantum systems which do not thermalize; i.e., expectation values approach an (approximately) steady longtime limit which disagrees with the microcanonical prediction of equilibrium statistical mechanics. A general analytical theory is worked out for the typical temporal relaxation behavior in such cases. The main prerequisites are initial conditions which appreciably populate many energy levels and do not give rise to significant spatial inhomogeneities on macroscopic scales...
May 12, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28548523/strength-and-viscosity-effects-on-perturbed-shock-front-stability-in-metals
#8
S Opie, E Loomis, P Peralta, T Shimada, R P Johnson
Computational modeling and experimental measurements on metal samples subject to a laser-driven, ablative Richtmyer-Meshkov instability showed differences between viscosity and strength effects. In particular, numerical and analytical solutions, coupled with measurements of fed-through perturbations, generated by perturbed shock fronts onto initially flat surfaces, show promise as a validation method for models of deviatoric response in the postshocked material. Analysis shows that measurements of shock perturbation amplitudes at low sample thickness-to-wavelength ratios are not enough to differentiate between strength and viscosity effects, but that surface displacement data of the fed-through perturbations appears to resolve the ambiguity...
May 12, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28548516/relativistic-electron-streaming-instabilities-modulate-proton-beams-accelerated-in-laser-plasma-interactions
#9
S Göde, C Rödel, K Zeil, R Mishra, M Gauthier, F-E Brack, T Kluge, M J MacDonald, J Metzkes, L Obst, M Rehwald, C Ruyer, H-P Schlenvoigt, W Schumaker, P Sommer, T E Cowan, U Schramm, S Glenzer, F Fiuza
We report experimental evidence that multi-MeV protons accelerated in relativistic laser-plasma interactions are modulated by strong filamentary electromagnetic fields. Modulations are observed when a preplasma is developed on the rear side of a μm-scale solid-density hydrogen target. Under such conditions, electromagnetic fields are amplified by the relativistic electron Weibel instability and are maximized at the critical density region of the target. The analysis of the spatial profile of the protons indicates the generation of B>10  MG and E>0...
May 12, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28548282/estimating-the-effect-of-immortal-time-bias-in-urologic-research-a-case-example-of-testosterone-replacement-therapy
#10
Christopher J D Wallis, Refik Saskin, Steven A Narod, Calvin Law, Girish S Kulkarni, Arun Seth, Robert K Nam
OBJECTIVE: To quantify the effect of immortal-time bias in an observational study examining the effect of cumulative testosterone exposure on mortality. SUBJECTS AND METHODS: We used a population-based matched cohort study of men aged 66 and older newly treated with testosterone replacement therapy and matched-controls from 2007-2012 in Ontario, Canada to quantify the effects of immortal-time bias. We used generalized estimating equations to determine the association between cumulative testosterone replacement therapy exposure and mortality...
May 26, 2017: BJU International
https://www.readbyqxmd.com/read/28548205/reflections-on-klein-s-radical-notion-of-phantasy-and-its-implications-for-analytic-practice
#11
Rachel B Blass
Analysts may incorporate many of Melanie Klein's important contributions (e.g., on preoedipal dynamics, envy, and projective identification) without transforming their basic analytic approach. In this paper I argue that adopting the Kleinian notion of unconscious phantasy is transformative. While it is grounded in Freud's thinking and draws out something essential to his work, this notion of phantasy introduces a radical change that defines Kleinian thinking and practice and significantly impacts the analyst's basic clinical approach...
May 26, 2017: International Journal of Psycho-analysis
https://www.readbyqxmd.com/read/28548178/stereospecific-control-of-the-metal-centred-chirality-of-rhodium-iii-and-iridium-iii-complexes-bearing-tetradentate-cnn-p-ligands
#12
María Carmona, Ricardo Rodríguez, Isabel Méndez, Vincenzo Passarelli, Fernando J Lahoz, Pilar García-Orduña, Daniel Carmona
Ligands LH1-LH3 have been prepared by two successive condensation/reduction steps. These ligands react with MCl3 (M = Rh, Ir) rendering the trichlorido complexes [MCl3(κ(3)N,N',P-LH)] (M = Rh, LH = LH1 (1), LH2 (2), LH3 (3); M = Ir, LH = LH1, (4)) as racemic mixtures of fac and mer isomers. Only one of the two possible fac isomers was detected. The mer isomer of the rhodium compounds 1-3 quantitatively isomerizes to the more stable fac isomer, whereas the mer isomer of the iridium complex 4 does not. DFT calculations indicate a dissociative pathway for this isomerization...
May 26, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28547995/adsorption-induced-deformation-of-hierarchically-structured-mesoporous-silica-effect-of-pore-level-anisotropy
#13
Christian Balzer, Anna M Waag, Stefan Gehret, Gudrun Reichenauer, Florian Putz, Nicola Hüsing, Oskar Paris, Noam Bernstein, Gennady Y Gor, Alexander V Neimark
The goal of this work is to understand adsorption-induced deformation of hierarchically structured porous silica exhibiting well-defined cylindrical mesopores. For this purpose, we performed an in situ dilatometry measurement on a calcined and sintered monolithic silica sample during the adsorption of N2 at 77 K. To analyze the experimental data, we extended the adsorption stress model to account for the anisotropy of cylindrical mesopores, i.e., we explicitly derived the adsorption stress tensor components in the axial and radial direction of the pore...
May 26, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28547842/a-rapid-analytical-method-of-major-milk-proteins-by%C3%A2-reversed-phase-high-performance-liquid-chromatography
#14
Lu Ma, Yongxin Yang, Jingting Chen, Jiaqi Wang, Dengpan Bu
A reversed-phase high-performance liquid chromatography (RP-HPLC) method with rapid and automated analysis, good separation, high resolution, high accuracy and reproducible results was successfully developed and used to separate and quantify the major cow milk proteins within 30 min analytical time. Standard solutions of single purified cow milk proteins were used to develop calibration equations. The RP-HPLC method was validated with respect to intra-day repeatability, inter-day precision, linearity, accuracy and limit of detection (LOD)...
May 26, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28547821/effects-of-sleep-on-substance-use-in-adolescents-a-longitudinal-perspective
#15
Tam T Nguyen-Louie, Ty Brumback, Matthew J Worley, Ian M Colrain, Georg E Matt, Lindsay M Squeglia, Susan F Tapert
Substance use (SU) and sleep problems appear interrelated, but few studies have examined the influence of adolescent sleep patterns on development of SU disorders. This study prospectively examined the influence of sleep habits on subsequent SU in youth who later transitioned into heavy drinking. At time 1 (T1), participants (n = 95) were substance-naive 12- to 14-year-olds. Path-analytic models examined whether the effects of T1 risk factors (familial SU disorder, inhibition control, and externalizing and internalizing traits) on time 3 (M = 19...
May 26, 2017: Addiction Biology
https://www.readbyqxmd.com/read/28547211/sheldon-spectrum-and-the-plankton-paradox-two-sides-of-the-same-coin-a-trait-based-plankton-size-spectrum-model
#16
José A Cuesta, Gustav W Delius, Richard Law
The Sheldon spectrum describes a remarkable regularity in aquatic ecosystems: the biomass density as a function of logarithmic body mass is approximately constant over many orders of magnitude. While size-spectrum models have explained this phenomenon for assemblages of multicellular organisms, this paper introduces a species-resolved size-spectrum model to explain the phenomenon in unicellular plankton. A Sheldon spectrum spanning the cell-size range of unicellular plankton necessarily consists of a large number of coexisting species covering a wide range of characteristic sizes...
May 25, 2017: Journal of Mathematical Biology
https://www.readbyqxmd.com/read/28547184/combination-of-magnetic-dispersive-micro-solid-phase-extraction-and-supramolecular-solvent-based-microextraction-followed-by-high-performance-liquid-chromatography-for-determination-of-trace-amounts-of-cholesterol-lowering-drugs-in-complicated-matrices
#17
Somayeh Arghavani-Beydokhti, Maryam Rajabi, Alireza Asghari
A novel, efficient, rapid, simple, sensitive, selective, and environmentally friendly method termed magnetic dispersive micro solid-phase extraction combined with supramolecular solvent-based microextraction (Mdμ-SPE-SSME) followed by high-performance liquid chromatography (HPLC) with UV detection is introduced for the simultaneous microextraction of cholesterol-lowering drugs in complicated matrices. In the first microextraction procedure, using layered double hydroxide (LDH)-coated Fe3O4 magnetic nanoparticles, an efficient sample cleanup is simply and rapidly provided without the need for time-consuming centrifugation and elution steps...
May 26, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28546981/nurses-commitment-to-respecting-patient-dignity
#18
Zahra Raee, Heidarali Abedi, Mohsen Shahriari
BACKGROUND: Although respecting human dignity is a cornerstone of all nursing practices, industrialization has gradually decreased the attention paid to this subject in nursing care. Therefore, the present study aimed to investigate nurses' commitment to respecting patient dignity in hospitals of Isfahan, Iran. METHODS: This descriptive-analytical study was conducted in hospitals of Isfahan. Overall, 401 inpatients were selected by cluster sampling and then selected simple random sampling from different wards...
2017: Journal of Education and Health Promotion
https://www.readbyqxmd.com/read/28546968/assessment-of-nonscholastic-abilities-and-its-associated-factors-among-medical-students-an-exploratory-study
#19
S Ganesh Kumar, Sonali Sarkar
BACKGROUND: Nonscholastic abilities among medical students are an important area of concern for the health professionals. Very few studies had been conducted in the past with regard to it. OBJECTIVE: This study was an exploratory study aimed to assess the nonscholastic abilities among medical students in a medical institution in coastal South India. MATERIALS AND METHODS: This study assessed three broad domains of nonscholastic abilities namely personal qualities, interpersonal activities, and communication skills among 106 medical students using a structured questionnaire (27 questions with a total score of 27)...
2017: Journal of Education and Health Promotion
https://www.readbyqxmd.com/read/28546891/scaling-law-to-determine-peak-forces-in-tapping-mode-afm-experiments-on-finite-elastic-soft-matter-systems
#20
Horacio V Guzman
Analytical equations to estimate the peak force will facilitate the interpretation and the planning of amplitude-modulation force microscopy (tapping mode) experiments. A closed-form analytical equation to estimate the tip-sample peak forces while imaging soft materials in liquid environment and within an elastic deformation regime has been deduced. We have combined a multivariate regression method with input from the virial-dissipation equations and Tatara's bidimensional deformation contact mechanics model...
2017: Beilstein Journal of Nanotechnology
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