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https://www.readbyqxmd.com/read/29245117/gold-nanoflowers-synthesized-using-acanthopanacis-cortex-extract-inhibit-inflammatory-mediators-in-lps-induced-raw264-7-macrophages-via-nf-%C3%AE%C2%BAb-and-ap-1-pathways
#1
Sungeun Ahn, Priyanka Singh, Mi Jang, Yu-Jin Kim, Verónica Castro-Aceituno, Shakina Yesmin Simu, Yeon Ju Kim, Deok-Chun Yang
We reported the rapid synthesis (<8s) of gold nanoparticles at room temperature using Acanthopanacis cortex extract (A-AuNPs). We characterized the A-AuNPs using several analytical techniques and found that nano-flower type A-AuNPs, which are known to possess a coarse surface with a high surface to volume ratio, conferring these particles with high binding capacity for various biological molecules. After confirming the stability of the nanoparticles, we investigated the anti-inflammatory effect of A-AuNPs in LPS-stimulated RAW264...
November 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29245088/-ghost-peak-of-clofazimine-a-solution-degradation-product-of-clofazimine-via-nucleophilic-substitution-by-nitrite-leaching-from-certain-glass-hplc-vials
#2
Jinsheng Lin, Zhenghuai Lin, Dan Li, Mengxue Feng, Qin Yang, Wenping Hu, Yang Han, Wenquan Zhu, Min Li, LuShan Yu
Analytical solutions of clofazimine drug substance stored in glass HPLC vials were found to undergo degradation at room temperature occasionally. At the time of each sample preparation, it was unpredictable if a particular solution would undergo such solution degradation. Once the degradation peak was observed in a particular vial, typically within 24h, it would keep growing until reaching a total yield of approximately 2%. By using a strategy that combines LC-PDA/UV-MSn with mechanism-based stress studies, followed by preparative HPLC separation and subsequent structure characterization by 1D and 2D NMR, the unknown peak was identified as a clofazimine nitrite ester...
November 26, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29245084/application-of-analytical-quality-by-design-principles-for-the-determination-of-alkyl-p-toluenesulfonates-impurities-in-aprepitant-by-hplc-validation-using-total-error-concept
#3
Constantinos K Zacharis, Elli Vastardi
In the research presented we report the development of a simple and robust liquid chromatographic method for the quantification of two genotoxic alkyl sulphonate impurities (namely methyl p-toluenesulfonate and isopropyl p-toluenesulfonate) in Aprepitant API substances using the Analytical Quality by Design (AQbD) approach. Following the steps of AQbD protocol, the selected critical method attributes (CMAs) were the separation criterions between the critical peak pairs, the analysis time and the peak efficiencies of the analytes...
December 7, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29244974/the-use-of-an-antioxidant-dressing-on-hard-to-heal-wounds-a-multicentre-prospective-case-series
#4
B Castro, F D Bastida, T Segovia, P López Casanova, J J Soldevilla, J Verdú-Soriano
OBJECTIVE: Oxidative stress can contribute to impaired wound healing and chronic wounds. Our objective was to test the results of a new antioxidant dressing that could help stop the oxidative stress of cells in the wound bed. METHOD: A multicentre, prospective case study series was conducted in three Spanish hospitals. The RESVECH 2.0 index was used for healing assessment. Data from each patient was collected by the attending clinical researchers. Data analysis was performed using the statistical concept intention-to-treat (ITT)...
December 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/29244947/a-new-strategy-for-in-vitro-determination-of-carbonic-anhydrase-activity-from-analysis-of-oxygen-18-isotopes-of-co2
#5
Chiranjit Ghosh, Santanu Mandal, Mithun Pal, Manik Pradhan
The oxygen-18 isotopic (18O) composition in CO2 provides important insight into the variation of rate in isotopic fractionation reaction regulated by carbonic anydrase (CA) metalloenzyme. This work aims to employ 18O-isotope ratio-based analytical method for quantitative estimation of CA activity in erythrocytes for clinical testing purposes. Here, a new method has been developed that contains the measurements of 18O/16Oisotope ratios during oxygen-18 isotopic exchange between 12C16O16O and H218O of in-vitro biochemical reaction controlled by erythrocytes CA and estimation of enzymatic activity of CA from the isotopic composition of CO2...
December 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29244880/comparative-study-of-qualitative-and-quantitative-methods-to-determine-toxicity-level-of-aspergillus-flavus-isolates-in-maize
#6
Meena Shekhar, Nirupma Singh, Ram Dutta, Shrvan Kumar, Vinay Mahajan
An attempt was made to compare between easy and inexpensive qualitative method (ammonia vapour test) and analytical methods (thin layer chromatography and enzyme-linked immunosorbent assay) for identification of aflatoxigenic isolates of Aspergillus flavus in maize. In this comparative study the toxicity level of A. flavus isolates exhibited 100% agreement among ammonia vapour test, ELISA and TLC for highly toxigenic (>2000 ppb) and toxigenic (501-2000 ppb) isolates while 88.5% agreement observed for least toxic (<20 ppb) isolates...
2017: PloS One
https://www.readbyqxmd.com/read/29244790/lentivirus-mediated-silencing-of-the-ptc1-and-ptc2-genes-promotes-recovery-from-spinal-cord-injury-by-activating-the-hedgehog-signaling-pathway-in-a-rat-model
#7
Ya-Dong Zhang, Zhong-Sheng Zhu, Dong Zhang, Zhen Zhang, Bin Ma, Shi-Chang Zhao, Feng Xue
This study aimed to investigate the effect of Patched-1 (PTC1) and PTC2 silencing in a rat model, on Hedgehog (Hh) pathway-mediated recovery from spinal cord injury (SCI). An analytical emphasis on the relationship between the sonic hedgehog (Shh) pathway and nerve regeneration was explored. A total of 126 rats were divided into normal, sham, SCI, negative control (NC), PTC1-RNAi, PTC2-RNAi and PTC1/PTC2-RNAi groups. The Basso, Beattie and Bresnahan (BBB) scale was employed to assess hind limb motor function...
December 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29244784/factors-associated-to-inpatient-mortality-rates-in-type-2-diabetic-patients-a-cross-sectional-analytical-study-in-three-peruvian-hospitals
#8
Noé Atamari-Anahui, Franklin W Martinez-Ninanqui, Liz Paucar-Tito, Luz Morales-Concha, Alejandra Miranda-Chirau, Marco Antonio Gamarra-Contreras, Carlos Antonio Zea-Nuñez, Christian R Mejia
INTRODUCTION: Diabetes mortality has increased in recent years. In Peru, there are few studies on in-hospital mortality due to type 2 diabetes in the provinces. OBJECTIVE: To determine factors associated to hospital mortality in patients with diabetes mellitus type 2 in three hospitals from Cusco-Peru. METHODS: An analytical cross-sectional study was performed. All patients with diabetes mellitus type 2 hospitalized in the city of Cusco during the 2016 were included...
December 5, 2017: Medwave
https://www.readbyqxmd.com/read/29244765/new-insights-into-solid-form-stability-and-hydrate-formation-o-phenanthroline-hcl-and-neocuproine-hcl
#9
Doris E Braun, Katharina Raabe, Anna Schneeberger, Volker Kahlenberg, Ulrich J Griesser
The moisture- and temperature dependent stabilities and interrelation pathways of the practically relevant solid forms of o-phenanthroline HCl (1) and neocuproine HCl (2) were investigated using thermal analytical techniques (HSM, DSC and TGA) and gravimetric moisture sorption/desorption studies. The experimental stability data were correlated with the structural changes observed upon dehydration and the pairwise interaction and lattice energies calculated. For 1 the monohydrate was identified as the only stable form under conditions of RH typically found during production and storage, but at RH values >80% deliquescence occurs...
December 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29244756/recent-progress-in-biosensors-for-environmental-monitoring-a-review
#10
REVIEW
Celine I L Justino, Armando C Duarte, Teresa A P Rocha-Santos
The environmental monitoring has been one of the priorities at the European and global scale due to the close relationship between the environmental pollution and the human health/socioeconomic development. In this field, the biosensors have been widely employed as cost-effective, fast, in situ, and real-time analytical techniques. The need of portable, rapid, and smart biosensing devices explains the recent development of biosensors with new transduction materials, obtained from nanotechnology, and for multiplexed pollutant detection, involving multidisciplinary experts...
December 15, 2017: Sensors
https://www.readbyqxmd.com/read/29244511/molecular-rotors-for-universal-quantitation-of-nanoscale-hydrophobic-interfaces-in-microplate-format
#11
Paul William Bisso, Michelle Tai, Hari Katepalli, Nicolas Bertrand, Daniel Blankschtein, Robert Langer
Hydrophobic self-assembly pairs diverse chemical precursors and simple formulation processes to access a vast array of functional colloids. Exploration of this design space, however, is stymied by lack of broadly general, high-throughput colloid characterization tools. Here, we show that a narrow structural subset of fluorescent, zwitterionic molecular rotors - dialkylaminostilbazolium sulfonates [DASS] with intermediate-length alkyl tails - fills this major analytical void by quantitatively sensing hydrophobic interfaces in microplate format...
December 15, 2017: Nano Letters
https://www.readbyqxmd.com/read/29244215/development-of-a-validated-liquid-chromatographic-method-for-quantification-of-sorafenib-tosylate-in-the-presence-of-stress-induced-degradation-products-and-in-biological-matrix-employing-analytical-quality-by-design-aqbd-approach
#12
Teenu Sharma, Rajneet Kaur Khurana, Atul Jain, O P Katare, Bhupinder Singh
The current research work envisages AQbD-enabled development of a simple, rapid, sensitive, specific, robust and cost-effective stability indicating reversed phase high performance liquid chromatographic (RP-HPLC) method for determining stress-induced forced degradation products of sorafenib tosylate (SFN). An Ishikawa fish bone diagram was constructed to embark upon analytical target profile (ATP) and critical analytical attributes (CAAs) i.e., peak area, theoretical plates, retention time (Rt ) and peak tailing...
December 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29244082/quantitation-of-cocaine-and-metabolites-phencyclidine-butalbital-and-phenobarbital-in-meconium-by-liquid-chromatography-tandem-mass-spectrometry
#13
Triniti L Scroggin, Gwendolyn A McMillin
In this study, a quantitative polarity switching liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS) method was developed to detect and quantify cocaine and metabolites (cocaethylene, benzoylecgonine and meta-hydroxybenzoylecgonine), phencyclidine (PCP) and barbiturates (phenobarbital and butalbital) in meconium. Accuracy and precision samples at 0.0125% and 75% of the upper limit of quantitation (ULOQ) were analyzed in triplicate over 5 days with accuracy above 84% and average %CV values below 11%...
December 13, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/29244080/quantification-of-methadone-and-main-metabolites-in-nails
#14
Teresa Patrícia Magalhães, Sara Cravo, Diana Dias da Silva, Ricardo Jorge Dinis-Oliveira, Carlos Afonso, Maria de Lourdes Bastos, Helena Carmo
The quantification of drugs of abuse in keratinized matrices is becoming of special relevance for monitoring consumption and for post-mortem investigations. We aimed to implement an analytical method for the simultaneous detection of morphine (MORF), 6-monoacetylmorphine (6-MAM), methadone (MET), 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenylpyrrolidine (EMDP) in nails. After decontamination, the nail samples (30 mg) were submitted to an alkaline digestion followed by a two-step liquid-liquid and SPE extraction using mixed-mode cation exchange cartridges...
December 13, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/29244076/reference-brain-blood-concentrations-of-citalopram-duloxetine-mirtazapine-and-sertraline
#15
Michael Nedahl, Sys Stybe Johansen, Kristian Linnet
Postmortem blood samples may not accurately reflect antemortem drug concentrations, as the levels of some drugs increase due to postmortem redistribution (PMR). The brain has been suggested as an alternative sampling site. The anatomically secluded site of the brain limits redistribution and prolongs the detection window, thereby enabling sampling from deceased individuals where blood is no longer suitable for analysis. We report concentrations in brain tissue and blood from 91 cases for the four antidepressants citalopram, duloxetine, mirtazapine and sertraline...
December 13, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/29244051/towards-microwave-imaging-of-cells
#16
Mehmet Kelleci, Hande Aydogmus, Levent Aslanbas, Selcuk Oguz Erbil, M Selim Hanay
Integrated detection techniques that can characterize the morphological properties of cells are needed for the widespread use of lab-on-a-chip technology. Herein, we establish a theoretical and experimental framework to use resonant microwave sensors in their higher order modes so that the morphological properties of analytes inside a microfluidic channel can be obtained electronically. We built a phase-locked loop system that can track the first two modes of a microstrip line resonator to detect the size and location of microdroplets and cells passing through embedded microfluidic channels...
December 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29244019/prevalence-healthcare-resource-utilization-and-overall-burden-of-fungal-meningitis-in-the-united-states
#17
Lefko T Charalambous, Alykhan Premji, Caroline Tybout, Anastasia Hunt, Drew Cutshaw, Aladine A Elsamadicy, Siyun Yang, Jichun Xie, Charles Giamberardino, Promila Pagadala, John R Perfect, Shivanand P Lad
PURPOSE: Previous epidemiological and cost studies of fungal meningitis have largely focused on single pathogens, leading to a poor understanding of the disease in general. We studied the largest and most diverse group of fungal meningitis patients to date, over the longest follow-up period, to examine the broad impact on resource utilization within the United States. METHODOLOGY: The Truven Health Analytics MarketScan database was used to identify patients with a fungal meningitis diagnosis in the United States between 2000 and 2012...
December 15, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29244018/detection-of-novel-betapapillomaviruses-and-gammapapillomaviruses-in-eyebrow-hair-follicles-using-a-single-tube-hanging-droplet-pcr-assay-with-modified-pan-pv-codehop-primers
#18
Diego Chouhy, Boštjan J Kocjan, Jeannette P Staheli, Elisa M Bolatti, Lea Hošnjak, Martin Sagadin, Adriana A Giri, Timothy M Rose, Mario Poljak
A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60 % (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types...
December 15, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29243755/efficient-skyrmion-transport-mediated-by-a-voltage-controlled-magnetic-anisotropy-gradient
#19
Xuan Wang, W L Gan, J C Martinez, F N Tan, M B A Jalil, W S Lew
Despite the inefficiencies associated with current-induced spin torques, they remain the predominant mode of skyrmion propulsion. In this work, we demonstrate numerically that skyrmions can be transported much more efficiently with a voltage-controlled magnetic anisotropy (VCMA) gradient. An analytical model was developed to understand the underlying skyrmion dynamics on a track under the VCMA conditions. Our calculations reveal that the repulsive skyrmion-edge interaction not only prevents the skyrmion from annihilating but also generates most of the skyrmion propulsion...
December 15, 2017: Nanoscale
https://www.readbyqxmd.com/read/29243668/chemotactic-droplet-swimmers-in-complex-geometries
#20
Chenyu Jin, Babak Vajdi Hokmabad, Kyle Anthony Baldwin, Corinna Charlotte Maass
Chemotaxis and auto-chemotaxis are key mechanisms in the dynamics of micro-organisms, e.g. in the acquisition of nutrients and in the communication between individuals, influencing the collective behaviour. However, chemical signalling and the natural environment of biological swimmers are generally complex, making them hard to access analytically. We present a well-controlled, tunable artificial model to study chemotaxis and autochemotaxis in complex geometries, using microfluidic assays of self-propelling oil droplets in an aqueous surfactant solution...
December 15, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
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