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https://www.readbyqxmd.com/read/29980701/comparative-and-phylogenetic-analysis-of-a-novel-family-of-enterobacteriaceae-associated-genomic-islands-that-share-a-conserved-excision-integration-module
#1
Alejandro Piña-Iturbe, Diego Ulloa-Allendes, Catalina Pardo-Roa, Irenice Coronado-Arrázola, Francisco J Salazar-Echegarai, Bianca Sclavi, Pablo A González, Susan M Bueno
Genomic Islands (GIs) are DNA regions acquired through horizontal gene transfer that encode advantageous traits for bacteria. Many GIs harbor genes that encode the molecular machinery required for their excision from the bacterial chromosome. Notably, the excision/integration dynamics of GIs may modulate the virulence of some pathogens. Here, we report a novel family of GIs found in plant and animal Enterobacteriaceae pathogens that share genes with those found in ROD21, a pathogenicity island whose excision is involved in the virulence of Salmonella enterica serovar Enteritidis...
July 6, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29946315/-acinetobacter-baumannii-epidemiological-and-beta-lactamase-data-from-two-tertiary-academic-hospitals-in-tshwane-south-africa
#2
Michelle Lowe, Marthie M Ehlers, Farzana Ismail, Gisele Peirano, Piet J Becker, Johann D D Pitout, Marleen M Kock
Acinetobacter baumannii is an opportunistic pathogen that is increasingly responsible for hospital-acquired infections. The increasing prevalence of carbapenem resistant A. baumannii has left clinicians with limited treatment options. Last line antimicrobials (i.e., polymyxins and glycylcyclines) are often used as treatment options. The aim of this study was to determine the prevalence of selected β-lactamase genes from A. baumannii isolates obtained from patients with hospital-acquired infections and to determine the genetic relationship and epidemiological profiles among clinical A...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29925644/multiplex-pcr-analysis-for-the-rapid-detection-of-klebsiella-pneumoniae-carbapenem-resistant-st258-11-and-hypervirulent-st23-65-86-375-strains
#3
Fangyou Yu, Jingnan Lv, Siqiang Niu, Hong Du, Yi-Wei Tang, Johann D D Pitout, Robert A Bonomo, Barry N Kreiswirth, Liang Chen
Carbapenem-resistant and hypervirulent Klebsiella pneumoniae have emerged recently. These strains are both hypervirulent and multidrug resistant, and may also be highly transmissible and able to cause severe infections in both the hospital and in the community. Clinical and public health need is required for a rapid and comprehensive molecular detection assay to identify and track their spread and provide timely infection control information. Here we develop a rapid multiplex PCR assay capable of distinguishing K...
June 20, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29891602/clonal-background-resistance-gene-profile-and-porin-gene-mutations-modulate-in-vitro-susceptibility-to-imipenem-relebactam-in-diverse-enterobacteriaceae
#4
Angela Gomez-Simmonds, Stephania Stump, Marla J Giddins, Medini K Annavajhala, Anne-Catrin Uhlemann
Treatment options for carbapenem-resistant Enterobacteriaceae (CRE) are limited. While Klebsiella pneumoniae harboring bla KPC account for most CRE, recent evidence points to increasing diversification of CRE. We determined whether CRE species and antibiotic resistance genotype influence response to relebactam (REL), a novel beta-lactamase inhibitor with class A/C activity, combined with imipenem(IMI)/cilastatin. We carried out broth microdilution testing to IMI alone or in the presence of 4 μg/mL REL in 154 clinical isolates collected at a New York City hospital with high prevalence of bla KPC including Enterobacter spp...
June 11, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29764953/fighting-antibiotic-resistant-klebsiella-pneumoniae-with-sweet-immune-targets
#5
Roberto Adamo, Immaculada Margarit
Antibiotics and vaccines have greatly impacted human health in the last century by dramatically reducing the morbidity and mortality associated with infectious diseases. The recent challenge posed by the emergence of multidrug-resistant bacteria could possibly be addressed by novel immune prophylactic and therapeutic approaches. Among the newly threatening pathogens, Klebsiella pneumoniae is particularly worrisome in the nosocomial setting, and its surface polysaccharides are regarded as promising antigen candidates...
May 15, 2018: MBio
https://www.readbyqxmd.com/read/29744043/genome-based-analysis-of-carbapenemase-producing-klebsiella-pneumoniae-isolates-from-german-hospital-patients-2008-2014
#6
Laura Becker, Martin Kaase, Yvonne Pfeifer, Stephan Fuchs, Annicka Reuss, Anja von Laer, Muna Abu Sin, Miriam Korte-Berwanger, Sören Gatermann, Guido Werner
Background: By using whole genome sequence data we aimed at describing a population snapshot of carbapenemase-producing K. pneumoniae isolated from hospitalized patients in Germany between 2008 and 2014. Methods: We selected a representative subset of 107 carbapenemase-producing K. pneumoniae clinical isolates possessing the four most prevalent carbapenemase types in Germany (KPC-2, KPC-3, OXA-48, NDM-1). Isolates were processed via illumina NGS. Data were analysed using different SNP-based mapping and de-novo assembly approaches...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29688339/4-5-years-within-patient-evolution-of-a-colistin-resistant-kpc-producing-klebsiella-pneumoniae-st258
#7
Agnès B Jousset, Rémy A Bonnin, Isabelle Rosinski-Chupin, Delphine Girlich, Gaëlle Cuzon, Nicolas Cabanel, Hélène Frech, Eric Farfour, Laurent Dortet, Philippe Glaser, Thierry Naas
Background: KPC-producing Klebsiella pneumoniae (KPC-Kp) have emerged globally over the last decade as a major nosocomial pathogen that threatens patient's care. These highly resistant bacteria are mostly associated with a single Kp clonal group (CG) CG258, but the reasons for its host and hospital adaptation remain largely unknown. Methods: We analyzed the in vivo evolution of a colistin-resistant KPC-Kp-CG258 strain that contaminated a patient following an endoscopy, and was responsible for a fatal bacteraemia 4...
April 21, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29669884/iron-acquisition-and-siderophore-release-by-carbapenem-resistant-sequence-type-258-klebsiella-pneumoniae
#8
Victoria I Holden, Meredith S Wright, Sébastien Houle, Abigail Collingwood, Charles M Dozois, Mark D Adams, Michael A Bachman
Klebsiella pneumoniae is rapidly acquiring resistance to all known antibiotics, including carbapenems. Multilocus sequence type ST258 (sequence type 258), carrying a gene encoding the K. pneumoniae carbapenemase ( bla KPC ) on a transmissible plasmid, is the most prevalent carbapenem-resistant Enterobacteriaceae (CRE) in the United States and has disseminated worldwide. Previously, whole-genome sequencing identified core genome single nucleotide variants that divide ST258 into two distinct clades, ST258a and ST258b...
April 25, 2018: MSphere
https://www.readbyqxmd.com/read/29615497/novel-broadly-reactive-anticapsular-antibodies-against-carbapenem-resistant-klebsiella-pneumoniae-protect-from-infection
#9
Elizabeth Diago-Navarro, Michael P Motley, Gonzalo Ruiz-Peréz, Winnie Yu, Julianne Austin, Bruna M S Seco, Guozhi Xiao, Aniska Chikhalya, Peter H Seeberger, Bettina C Fries
Carbapenem-resistant (CR) sequence type 258 (ST258) Klebsiella pneumoniae has become an urgent health care threat, causing an increasing number of high-mortality infections. Its resistance to numerous antibiotics and threat to immunocompromised patients necessitate finding new therapies to combat these infections. Previous successes in the laboratory, as well as the conservation of capsular polysaccharide (CPS) among the members of the ST258 clone, suggest that monoclonal antibody (MAb) therapy targeting the outer polysaccharide capsule of K...
April 3, 2018: MBio
https://www.readbyqxmd.com/read/29593660/genetic-platforms-of-bla-ctx-m-in-carbapenemase-producing-strains-of-k-pneumoniae-isolated-in-chile
#10
Sergio Carrasco-Anabalón, Alejandra Vera-Leiva, Mario Quezada-Aguiluz, María F Morales-Rivera, Celia A Lima, Jorge Fernández, Soledad Ulloa, Mariana Domínguez, Gerardo González-Rocha, Helia Bello-Toledo
Objective: To elucidate whether the genetic platforms of bla CTX-M contribute to the phenotypes of multi-drug-resistance (MDR) in the first carbapenemase-producing K. pneumoniae strains isolated in Chile. Method: Twenty-two carbapenemase-producing K. pneumoniae strains isolated from different Chilean patients and hospitals were studied. Their genetic relatedness was assessed by PFGE and MLST. The levels of antibiotic resistance were evaluated by determining the minimum inhibitory concentration of various antimicrobials...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29535199/antibody-mediated-killing-of-carbapenem-resistant-st258-klebsiella-pneumoniae-by-human-neutrophils
#11
Scott D Kobayashi, Adeline R Porter, Brett Freedman, Ruchi Pandey, Liang Chen, Barry N Kreiswirth, Frank R DeLeo
Carbapenem-resistant Klebsiella pneumoniae is a problem worldwide. A carbapenem-resistant K. pneumoniae lineage classified as multilocus sequence type 258 (ST258) is prominent in the health care setting in many regions of the world, including the United States. ST258 strains can be resistant to virtually all clinically useful antibiotics; treatment of infections caused by these organisms is difficult, and mortality is high. As a step toward promoting development of new therapeutics for ST258 infections, we tested the ability of rabbit antibodies specific for ST258 capsule polysaccharide to enhance human serum bactericidal activity and promote phagocytosis and killing of these bacteria by human neutrophils...
March 13, 2018: MBio
https://www.readbyqxmd.com/read/29507064/pneumonia-and-renal-replacement-therapy-are-risk-factors-for-ceftazidime-avibactam-treatment-failures-and-resistance-among-patients-with-carbapenem-resistant-enterobacteriaceae-infections
#12
Ryan K Shields, M Hong Nguyen, Liang Chen, Ellen G Press, Barry N Kreiswirth, Cornelius J Clancy
Ceftazidime-avibactam was used to treat 77 patients with carbapenem-resistant Enterobacteriaceae (CRE) infections at our center. Thirty- and 90-day survival rates were 81% and 69%, respectively; these rates were higher than those predicted by SAPS II and SOFA scores at the onset of infection. Clinical success was achieved for 55% of patients but differed by the site of infection. Success rates were lowest for pneumonia (36%) and higher for bacteremia (75%) and urinary tract infections (88%). By multivariate analysis, pneumonia ( P = 0...
May 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29486233/polymyxin-b-and-fosfomycin-thwart-kpc-producing-klebsiella-pneumoniae-in-the-hollow-fibre-infection-model
#13
Zackery P Bulman, Miao Zhao, Michael J Satlin, Liang Chen, Barry N Kreiswirth, Thomas J Walsh, Roger L Nation, Jian Li, Brian T Tsuji
Polymyxin B and fosfomycin are two 'old' antibiotics that consistently maintain activity against Klebsiella pneumoniae carbapenemase (KPC)-producing organisms based on in vitro susceptibility testing. However, each antibiotic's use in monotherapy has been associated with high rates of treatment failure. Therefore, our objective was to investigate the combinatorial pharmacodynamics of polymyxin B and fosfomycin against KPC-producing K. pneumoniae. Polymyxin B front-loading (3.33 mg/kg for 1 dose followed by 1...
February 24, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29431605/multifactorial-chromosomal-variants-regulate-polymyxin-resistance-in-extensively-drug-resistant-klebsiella-pneumoniae
#14
Miranda E Pitt, Alysha G Elliott, Minh Duc Cao, Devika Ganesamoorthy, Ilias Karaiskos, Helen Giamarellou, Cely S Abboud, Mark A T Blaskovich, Matthew A Cooper, Lachlan J M Coin
Extensively drug-resistant Klebsiella pneumoniae (XDR-KP) infections cause high mortality and are disseminating globally. Identifying the genetic basis underpinning resistance allows for rapid diagnosis and treatment. XDR isolates sourced from Greece and Brazil, including 19 polymyxin-resistant and five polymyxin-susceptible strains, were subjected to whole genome sequencing. Seventeen of the 19 polymyxin-resistant isolates harboured variations upstream or within mgrB. The most common mutation identified was an insertion at nucleotide position 75 in mgrB via an ISKpn26-like element in the ST258 lineage and ISKpn13 in one ST11 isolate...
February 12, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29424684/genome-analysis-of-clinical-multilocus-sequence-type-11-klebsiella-pneumoniae-from-china
#15
Ning Dong, Rong Zhang, Lizhang Liu, Ruichao Li, Dachuan Lin, Edward Wai-Chi Chan, Sheng Chen
The increasing prevalence of KPC-producing Klebsiella pneumoniae strains in clinical settings has been largely attributed to dissemination of organisms of specific multilocus sequence types, such as ST258 and ST11. Compared with the ST258 clone, which is prevalent in North America and Europe, ST11 is common in China but information regarding its genetic features remains scarce. In this study, we performed detailed genetic characterization of ST11 K. pneumoniae strains by analyzing whole-genome sequences of 58 clinical strains collected from diverse geographic locations in China...
February 8, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29263067/successive-emergence-of-ceftazidime-avibactam-resistance-through-distinct-genomic-adaptations-in-bla-kpc-2-harboring-klebsiella-pneumoniae-sequence-type-307-isolates
#16
Marla J Giddins, Nenad Macesic, Medini K Annavajhala, Stephania Stump, Sabrina Khan, Thomas H McConville, Monica Mehta, Angela Gomez-Simmonds, Anne-Catrin Uhlemann
Ceftazidime-avibactam (CAZ-AVI) is a promising novel treatment for infections caused by carbapenem-resistant Enterobacteriaceae (CRE). Despite improved treatment outcomes compared to those achieved with aminoglycoside- and colistin-based regimens, the rapid evolution of CAZ-AVI resistance during treatment has previously been reported in Klebsiella pneumoniae sequence type 258 (ST258) bla KPC-3 -harboring isolates. Here, we report the stepwise evolution and isolation of two phenotypically distinct CAZ-AVI-resistant Klebsiella pneumoniae isolates from a patient with pancreatitis...
March 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29236574/genetic-diversity-of-kpc-producing-escherichia-coli-klebsiella-oxytoca-serratia-marcescens-and-citrobacter-freundii-isolates-from-argentina
#17
Denise De Belder, Celeste Lucero, Melina Rapoport, Adriana Rosato, Diego Faccone, Alejandro Petroni, Fernando Pasteran, Ezequiel Albornoz, Alejandra Corso, Sonia A Gomez
The predominance of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae was caused by the spread of ST258 clone. In Latin America, KPC was reported in 2006, with the isolation of genetically unrelated K. pneumoniae in Colombia. Since then, the expansion of blaKPC in either K. pneumoniae ST258 or other Enterobacteriaceae (ETB) species was increasingly reported. In this study, we characterized 89 KPC-producing Escherichia coli, Klebsiella oxytoca, Serratia marcescens, and Citrobacter freundii that were received between 2010 and 2014...
December 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29176971/occurrence-of-extended-spectrum-%C3%AE-lactamases-kpc-type-and-mcr-1-2-producing-enterobacteriaceae-from-wells-river-water-and-wastewater-treatment-plants-in-oltrep%C3%A3-pavese-area-northern-italy
#18
Mariasofia Caltagirone, Elisabetta Nucleo, Melissa Spalla, Francesca Zara, Federica Novazzi, Vittoria M Marchetti, Aurora Piazza, Ibrahim Bitar, Marica De Cicco, Stefania Paolucci, Giorgio Pilla, Roberta Migliavacca, Laura Pagani
To evaluate the water compartment antibiotic-resistance contamination rates, 11 wells, five streams, and four treatment plants located in the Oltrepò Pavese area were screened for the presence of third generation cephalosporins resistant Gram-negative bacteria. Enterobacteriaceae were also characterized for the Extended-Spectrum-β-Lactamases (ESBLs), carbapenemases, and mcr-1 genes presence. From December 2014 to November 2015, 246 water samples were filtered, plated on Plate Count Agar, MacConkey Agar, and MacConkey Agar with cefotaxime...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29102103/frequency-antimicrobial-susceptibility-and-clonal-distribution-of-methicillin-resistant-staphylococcus-pseudintermedius-in-canine-clinical-samples-submitted-to-a-veterinary-diagnostic-laboratory-in-italy-a-3-year-retrospective-investigation
#19
G Ventrella, A Moodley, E Grandolfo, A Parisi, M Corrente, D Buonavoglia, L Guardabassi
In the last decade there has been a rapid global spread of methicillin-resistant Staphylococcus pseudintermedius (MRSP) clones displaying multidrug resistance in dogs. We investigated prevalence, antimicrobial susceptibility and clonal distribution of MRSP isolated from clinical canine samples between during 2011-2014. Following species identification by nuc PCR, MRSP were confirmed by the presence of mecA and characterized by antimicrobial susceptibility testing, Pulsed Field Gel Electrophoresis (PFGE), SCCmec typing, and Multi-Locus Sequence Typing (MLST) of a few isolates having distinct PFGE profiles...
November 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29097338/expansion-of-kpc-producing-klebsiella-pneumoniae-with-various-mgrb-mutations-giving-rise-to-colistin-resistance-the-role-of-isl3-on-plasmids
#20
Cesira Giordano, Simona Barnini, Constantinos Tsioutis, Monika A Chlebowicz, Effie V Scoulica, Achilleas Gikas, John W Rossen, Alexander W Friedrich, Erik Bathoorn
mcr-1 has been reported as the first plasmid-encoded gene conferring colistin resistance. In KPC-producing Klebsiella pneumoniae (KPC-KP), however, colistin resistance is rapidly emerging through other mechanisms. Resistance is frequently due to disruption of the mgrB gene by insertion sequences, e.g. ISL3. The aim of this study was to investigate the expansion of mgrB-mutated KPC-KP isolates. In addition, the localisation and targets of ISL3 sequences within the core and accessory genome of common KPC-KP lineages were identified...
February 2018: International Journal of Antimicrobial Agents
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