keyword
https://read.qxmd.com/read/38486755/designing-a-conjugate-vaccine-targeting-klebsiella-pneumoniae-st258-and-st11
#1
JOURNAL ARTICLE
Min Li, Mingkai Yu, Yigang Yuan, Danyang Li, Daijiao Ye, Min Zhao, Zihan Lin, Liuzhi Shi
Klebsiella pneumoniae ( K. pneumoniae ) is a common bacterium that can cause iatrogenic infection. Recently, the rise of antibiotic resistance among K. pneumoniae strains is one key factor associated with antibiotic treatment failure. Hencefore, there is an urgent need for effective K. pneumoniae vaccines. This study aimed to design a multi-epitope vaccine (MEV) candidate against K. pneumonia by utilizing an immunoinformatics method. In this study, we obtained 15 cytotoxic T lymphocyte epitopes, 10 helper T lymphocyte epitopes, 6 linear B-cell epitopes, and 2 conformational B-cell epitopes for further research...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38381174/carbapenem-resistant-klebsiella-pneumoniae-in-the-balkans-clonal-distribution-and-associated-resistance-determinants
#2
REVIEW
Maria Chatzidimitriou, Asimoula Kavvada, Dimitrios Kavvadas, Maria Anna Kyriazidi, Konstantinos Eleftheriadis, Sotirios Varlamis, Vassilis Papaliagkas, Stella Mitka
Carbapenems are considered to be among the last line antibiotics against extended-spectrum β-lactamase producing Enterobacterales. Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been frequently reported and its spread in Europe is indisputable and poses an enormous threat to hospitalized patients which is of growing concern. This review aims to record prevalence of CRKP in the Balkan region and to review the current knowledge about this life-threatening pathogen. In this review, we summarize data about clinical isolates of carbapenem-resistant K...
February 21, 2024: Acta Microbiologica et Immunologica Hungarica
https://read.qxmd.com/read/38319084/kpc-2-allelic-variants-in-klebsiella-pneumoniae-isolates-resistant-to-ceftazidime-avibactam-from-argentina-bla-kpc-80-bla-kpc-81-bla-kpc-96-and-bla-kpc-97
#3
JOURNAL ARTICLE
María Belén Sanz, Fernando Pasteran, Juan Manuel de Mendieta, Florencia Brunetti, Ezequiel Albornoz, Melina Rapoport, Celeste Lucero, Laura Errecalde, Maria Rosa Nuñez, Renata Monge, Magdalena Pennini, Pablo Power, Alejandra Corso, Sonia A Gomez
Ceftazidime-avibactam (CZA) therapy has significantly improved survival rates for patients infected by carbapenem-resistant bacteria, including KPC producers. However, resistance to CZA is a growing concern, attributed to multiple mechanisms. In this study, we characterized four clinical CZA-resistant Klebsiella pneumoniae isolates obtained between July 2019 and December 2020. These isolates expressed novel allelic variants of bla KPC-2 resulting from changes in hotspots of the mature protein, particularly in loops surrounding the active site of KPC...
February 6, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38313058/differential-mucosal-tropism-and-dissemination-of-classical-and-hypervirulent-klebsiella-pneumoniae-infection
#4
JOURNAL ARTICLE
Teck-Hui Teo, Nurul N Ayuni, Michelle Yin, Jun Hao Liew, Jason Q Chen, Natalia Kurepina, Ravisankar Rajarethinam, Barry N Kreiswirth, Liang Chen, Pablo Bifani
Klebsiella pneumoniae (Kp) infection is an important healthcare concern. The ST258 classical (c)Kp strain is dominant in hospital-acquired infections in North America and Europe, while ST23 hypervirulent (hv)Kp prevails in community-acquired infections in Asia. This study aimed to develop symptomatic mucosal infection models in mice that mirror natural infections in humans to gain a deeper understanding of Kp mucosal pathogenesis. We showed that cKp replicates in the nasal cavity instead of the lungs, and this early infection event is crucial for the establishment of chronic colonization in the cecum and colon...
February 16, 2024: IScience
https://read.qxmd.com/read/38247640/in-vitro-antimicrobial-activity-of-five-newly-approved-antibiotics-against-carbapenemase-producing-enterobacteria-a-pilot-study-in-bulgaria
#5
JOURNAL ARTICLE
Rumyana Markovska, Petya Stankova, Temenuga Stoeva, Emma Keuleyan, Kalina Mihova, Lyudmila Boyanova
To solve the problem with pan-drug resistant and extensively drug-resistant Gram-negative microbes, newly approved drugs such as ceftazidime/avibactam, cefiderocol, plazomicin, meropenem/vaborbactam, and eravacycline have been introduced in practice. The aim of the present study was to collect carbapenemase-producing clinical Enterobacterales isolates, to characterize their carbapenemase genes and clonal relatedness, and to detect their susceptibility to commonly used antimicrobials and the above-mentioned newly approved antibiotics...
January 15, 2024: Antibiotics
https://read.qxmd.com/read/38157139/activity-of-imipenem-relebactam-and-comparators-against-kpc-producing-klebsiella-pneumoniae-and-imipenem-resistant-pseudomonas-aeruginosa
#6
JOURNAL ARTICLE
Mercedes Delgado-Valverde, Inés Portillo-Calderón, Manuel Alcalde-Rico, M Carmen Conejo, Carmen Hidalgo, Carlos Del Toro Esperón, Álvaro Pascual
PURPOSE: Relebactam is a novel β-lactamase inhibitor, which, when combined with imipenem/cilastatin, is active against both class A and class C β-lactamases. To evaluate in vitro antimicrobial activity of imipenem/relebactam against a collection of recent clinical isolates of carbapenem-non-susceptible P. aeruginosa and K. pneumoniae ST258 and ST512 KPC producers belonging to different lineages from hospitals in Southern Spain. METHODS: Six hundred and seventy-eight isolates were tested: 265 K...
December 29, 2023: European Journal of Clinical Microbiology & Infectious Diseases
https://read.qxmd.com/read/38081547/global-genomic-profiling-of-klebsiella-pneumoniae-a-spatio-temporal-population-structure-analysis
#7
JOURNAL ARTICLE
Heng Heng, Xuemei Yang, Lianwei Ye, Yang Tang, Zhihao Guo, Jun Li, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Klebsiella pneumoniae (KP) has become the most important clinical bacterial pathogen following the emergence of hypervirulent and multidrug resistant variants. We used uniform Manifold Approximation and Projection (UMAP) to cluster genomes of 16,797 KP strains collected in over 100 countries during the period 1937-2021 based on core genome distance. A total of 60 high-density genetic clusters of strains representing the major epidemic strains were identified among these strains. Using UMAP bedding, the relationship between genetic cluster, KL types, ST types of the strains was clearly demonstrated, with some important ST types such as ST11 and ST258 being found to contain multiple clusters...
December 9, 2023: International Journal of Antimicrobial Agents
https://read.qxmd.com/read/38079200/subinhibitory-antibiotic-concentrations-promote-the-excision-of-a-genomic-island-carried-by-the-globally-spread-carbapenem-resistant-klebsiella-pneumoniae-sequence-type-258
#8
JOURNAL ARTICLE
Alejandro Piña-Iturbe, Guillermo Hoppe-Elsholz, Isidora D Suazo, Alexis M Kalergis, Susan M Bueno
The ICEKp258.2 genomic island (GI) has been proposed as an important factor for the emergence and success of the globally spread carbapenem-resistant Klebsiella pneumoniae sequence type (ST) 258. However, a characterization of this horizontally acquired element is lacking. Using bioinformatic and experimental approaches, we found that ICEKp258.2 is not confined to ST258 and ST512, but also carried by ST3795 strains and emergent invasive multidrug-resistant pathogens from ST1519. We also identified several ICEKp258...
December 2023: Microbial Genomics
https://read.qxmd.com/read/37997662/emergence-and-persistent-spread-of-carbapenemase-producing-klebsiella-pneumoniae-high-risk-clones-in-greek-hospitals-2013-to-2022
#9
JOURNAL ARTICLE
Kyriaki Tryfinopoulou, Marius Linkevicius, Olga Pappa, Erik Alm, Kleon Karadimas, Olov Svartström, Michalis Polemis, Kassiani Mellou, Antonis Maragkos, Alma Brolund, Inga Fröding, Sophia David, Alkiviadis Vatopoulos, Daniel Palm, Dominique L Monnet, Theoklis Zaoutis, Anke Kohlenberg
BackgroundPreliminary unpublished results of the survey of carbapenem- and/or colistin-resistant Enterobacterales (CCRE survey) showed the expansion of carbapenemase-producing Klebsiella pneumoniae (CPKP) sequence type (ST) 39 in 12 of 15 participating Greek hospitals in 2019.AimWe conducted a rapid survey to determine the extent of spread of CPKP high-risk clones in Greek hospitals in 2022 and compare the distribution of circulating CPKP clones in these hospitals since 2013.MethodsWe analysed whole genome sequences and epidemiological data of 310 K...
November 2023: Euro Surveillance
https://read.qxmd.com/read/37893766/the-genotyping-diversity-and-hemolytic-activity-of-cronobacter-spp-isolated-from-plant-based-food-products-in-poland
#10
JOURNAL ARTICLE
Monika Garbowska, Anna Berthold-Pluta, Lidia Stasiak-Różańska, Antoni Pluta, Stephen Forsythe, Ilona Stefańska
The present study aimed to determine the genotyping diversity and hemolytic properties of 24 strains of Cronobacter spp. (15 Cronobacter sakazakii , 6 Cronobacter malonaticus , 2 Cronobacter turicensis , and 1 Cronobacter condimenti ) isolated from commercial ready-to-eat leaf vegetables, sprouts, nuts, and dried fruits. The multilocus sequence typing (MLST) method was used to determine the sequence types (ST) and clonal complexes (CC) of these strains. The study demonstrated the high genotypic diversity of the Cronobacter genus bacteria isolated from plant-based foods...
October 23, 2023: Foods (Basel, Switzerland)
https://read.qxmd.com/read/37642647/three-klebsiella-pneumoniae-lineages-causing-bloodstream-infections-variably-dominated-within-a-greek-hospital-over-a-15-year-period
#11
JOURNAL ARTICLE
Ayorinde O Afolayan, Anastasia Rigatou, Hajo Grundmann, Angeliki Pantazatou, George Daikos, Sandra Reuter
Carbapenem-resistant Klebsiella pneumoniae (CRKP) has emerged as a major clinical and public health threat. The rapid dissemination of this pathogen is driven by several successful clones worldwide. We aimed to investigate the CRKP clonal lineages, their antibiotic resistance determinants and their potential transmissions in a tertiary care hospital located in Athens, Greece. Between 2003 and 2018, 392 CRKP isolates from bloodstream infections were recovered from hospitalized patients. Whole genome sequencing (WGS) was performed on the Illumina platform to characterize 209 of these isolates...
August 2023: Microbial Genomics
https://read.qxmd.com/read/37439675/distinct-origins-and-transmission-pathways-of-bla-kpc-enterobacterales-across-three-u-s-states
#12
JOURNAL ARTICLE
Zena Lapp, Rany Octaria, Sean M O'Malley, Tu Ngoc Nguyen, Hannah Wolford, Ryan Crawford, Christina Moore, Paula Snippes Vagnone, Diane Noel, Nadezhda Duffy, Ali Pirani, Linda S Thomas, Brittany Pattee, Claire Pearson, Sandra N Bulens, Sophie Hoffman, Marion Kainer, Melissa Anacker, James Meek, Isaac See, Kyle J Gontjes, Allison Chan, Ruth Lynfield, Meghan Maloney, Mary K Hayden, Evan Snitkin, Rachel B Slayton
Carbapenem-resistant Enterobacterales (CRE) are among the most concerning antibiotic resistance threats due to high rates of multidrug resistance, transmissibility in health care settings, and high mortality rates. We evaluated the potential for regional genomic surveillance to track the spread of bla KPC -carrying CRE (KPC-CRE) by using isolate collections from health care facilities in three U.S. states. Clinical isolates were collected from Connecticut (2017 to 2018), Minnesota (2012 to 2018), and Tennessee (2016 to 2017) through the U...
August 23, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37367193/genotypic-determination-of-extended-spectrum-%C3%AE-lactamases-and-carbapenemase-production-in-clinical-isolates-of-klebsiella-pneumoniae-in-southwest-nigeria
#13
JOURNAL ARTICLE
Gbolabo Odewale, Motunrayo Yemisi Jibola-Shittu, Olusola Ojurongbe, Rita Ayanbolade Olowe, Olugbenga Adekunle Olowe
INTRODUCTION: Klebsiella pneumoniae is a major pathogen implicated in healthcare-associated infections. Extended-spectrum β-lactamase (ESBL) and carbapenemase-producing K. pneumoniae isolates are a public health concern. This study investigated the existence of some ESBL and carbapenemase genes among clinical isolates of K. pneumoniae in Southwest Nigeria and additionally determined their circulating clones. MATERIALS AND METHODS: Various clinical samples from 420 patients from seven tertiary hospitals within Southwestern Nigeria were processed between February 2018 and July 2019...
June 20, 2023: Infectious Disease Reports
https://read.qxmd.com/read/37350993/new-bacteriophages-members-of-the-ackermannviridae-family-specific-for-klebsiella-pneumoniae-st258
#14
JOURNAL ARTICLE
Estefanía Tisalema-Guanopatín, Fausto Cabezas-Mera, Karla Nolivos-Rodríguez, Isabel Fierro, Lourdes Pazmiño, Daniel Garzon-Chavez, Alexis Debut, Karla Vizuete, Jorge Aníbal Reyes
BACKGROUND: Carbapenem-resistant Klebsiella pneumoniae , particularly isolates classified as sequence-type 258 (ST258), are multidrug-resistant strains that are strongly associated with poor-prognosis nosocomial infections, as current therapeutic options are limited and ineffective. In recent years, phage therapy has emerged as a promising treatment option for these scenarios. METHODOLOGY AND RESULTS: We report the isolation and characterization of three new phages against Klebsiella pneumoniae ST258 strains recovered from Machángara river wastewater...
June 1, 2023: PHAGE: therapy, applications, and research
https://read.qxmd.com/read/37289087/clinical-and-bacterial-characteristics-associated-with-glove-and-gown-contamination-by-carbapenem-resistant-klebsiella-pneumoniae-in-the-health-care-setting
#15
JOURNAL ARTICLE
Tracy H Hazen, Timileyin Adediran, Stephanie Hitchcock, Lyndsay M O'Hara, Lisa Pineles, Jane M Michalski, J Kristie Johnson, M Hong Nguyen, David P Calfee, Loren G Miller, Anthony D Harris, David A Rasko
Carbapenem-resistant Klebsiella pneumoniae (CRKp) is a pathogen of significant concern to public health, as it has become increasingly associated with difficult-to-treat community-acquired and hospital-associated infections. Transmission of K. pneumoniae between patients through interactions with shared health care personnel (HCP) has been described as a source of infection in health care settings. However, it is not known whether specific lineages or isolates of K. pneumoniae are associated with increased transmission...
June 8, 2023: Microbiology Spectrum
https://read.qxmd.com/read/37138637/genetic-diversity-and-antimicrobial-resistance-profiles-of-staphylococcus-pseudintermedius-associated-with-skin-and-soft-tissue-infections-in-companion-animals-in-lisbon-portugal
#16
JOURNAL ARTICLE
Catarina Morais, Sofia Santos Costa, Marta Leal, Bárbara Ramos, Mariana Andrade, Carolina Ferreira, Patrícia Abrantes, Constança Pomba, Isabel Couto
Staphylococcus pseudintermedius is the main bacterial pathogen of skin and soft-tissue infections (SSTIs) in companion animals. Antimicrobial resistance in this species is a growing public health concern. This study aims to characterize a collection of S. pseudintermedius causing SSTIs in companion animals, establishing the main clonal lineages and antimicrobial resistance traits. The collection corresponded to all S. pseudintermedius ( n  = 155) causing SSTIs in companion animals (dogs, cats and one rabbit) collected between 2014 and 2018 at two laboratories in Lisbon, Portugal...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/37024486/author-correction-ompk36-mediated-carbapenem-resistance-attenuates-st258-klebsiella-pneumoniae-in-vivo
#17
Joshua L C Wong, Maria Romano, Louise E Kerry, Hok-Sau Kwong, Wen-Wen Low, Stephen J Brett, Abigail Clements, Konstantinos Beis, Gad Frankel
No abstract text is available yet for this article.
April 6, 2023: Nature Communications
https://read.qxmd.com/read/36991368/the-distribution-characteristics-of-global-blaoxa-carrying-klebsiella-pneumoniae
#18
JOURNAL ARTICLE
Lingning Meng, Ziyao Liu, Chang Liu, Chuchu Li, Han Shen, Xiaoli Cao
OBJECTIVE: To analyze the distribution of blaOXA among global Klebsiella pneumoniae and the characteristics of blaOXA-carrying K. pneumoniae. MATERIALS AND METHODS: The genomes of global K. pneumoniae were downloaded from NCBI by Aspera software. After quality check, the distribution of blaOXA among the qualified genomes was investigated by annotation with the resistant determinant database. The phylogenetic tree was constructed for the blaOXA variants based on the single nucleotide polymorphism (SNP) to explore the evolutionary relationship between these variants...
March 29, 2023: BMC Infectious Diseases
https://read.qxmd.com/read/36986401/emergence-of-hyper-epidemic-clones-of-enterobacterales-clinical-isolates-co-producing-kpc-and-metallo-beta-lactamases-during-the-covid-19-pandemic
#19
JOURNAL ARTICLE
Diego Faccone, Sonia A Gomez, Juan Manuel de Mendieta, María Belén Sanz, Mariano Echegorry, Ezequiel Albornoz, Celeste Lucero, Paola Ceriana, Alejandra Menocal, Florencia Martino, Denise De Belder, Alejandra Corso, Fernando Pasterán
BACKGROUND: The global spread of carbapenemase-producing Enterobacterales has become an epidemiological risk for healthcare systems by limiting available antimicrobial treatments. The COVID-19 pandemic worsened this scenario, prompting the emergence of extremely resistant microorganisms. METHODS: Between March 2020 and September 2021, the NRL confirmed 82 clinical Enterobacterales isolates harboring a combination of bla KPC and MBL genes. Molecular typing was analyzed by PFGE and MLST...
March 18, 2023: Pathogens
https://read.qxmd.com/read/36978392/estimation-evaluation-and-characterization-of-carbapenem-resistance-burden-from-a-tertiary-care-hospital-pakistan
#20
JOURNAL ARTICLE
Aamir Jamal Gondal, Nakhshab Choudhry, Hina Bukhari, Zainab Rizvi, Shah Jahan, Nighat Yasmin
Carbapenem resistance has become major concern in healthcare settings globally; therefore, its monitoring is crucial for intervention efforts to halt resistance spread. During May 2019-April 2022, 2170 clinical strains were characterized for antimicrobial susceptibility, resistance genes, replicon and sequence types. Overall, 42.1% isolates were carbapenem-resistant, and significantly associated with Klebsiella pneumoniae ( K. pneumoniae ) ( p = 0.008) and Proteus species ( p = 0.043). Carbapenemases were detected in 82...
March 6, 2023: Antibiotics
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