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Danielle Ahn, Hernán Peñaloza, Zheng Wang, Matthew Wickersham, Dane Parker, Purvi Patel, Antonius Koller, Emily I Chen, Susan M Bueno, Anne-Catrin Uhlemann, Alice Prince
Adaptive changes in the genome of a locally predominant clinical isolate of the multidrug-resistant Klebsiella pneumoniae ST258 (KP35) were identified and help to explain the selection of this strain as a successful pulmonary pathogen. The acquisition of 4 new ortholog groups, including an arginine transporter, enabled KP35 to outcompete related ST258 strains lacking these genes. KP35 infection elicited a monocytic response, dominated by Ly6C(hi) monocytic myeloid-derived suppressor cells that lacked phagocytic capabilities, expressed IL-10, arginase, and antiinflammatory surface markers...
October 20, 2016: JCI Insight
Tolani F Olonisakin, Huihua Li, Zeyu Xiong, Elizabeth J K Kochman, Minting Yu, Yanyan Qu, Mei Hulver, Jay K Kolls, Claudette St Croix, Yohei Doi, Minh-Hong Nguyen, Robert M Q Shanks, Rama K Mallampalli, Valerian E Kagan, Anuradha Ray, Roy L Silverstein, Prabir Ray, Janet S Lee
Klebsiella pneumoniae remains an important cause of intrapulmonary infection and invasive disease worldwide. Klebsiella pneumoniae can evade serum killing and phagocytosis primarily through the expression of a polysaccharide capsule, but its pathogenicity is also influenced by host factors. We examined whether CD36, a scavenger receptor that recognizes pathogen and modified-self ligands, is a host determinant of Klebsiella pneumoniae pathogenicity. Despite differences in serum sensitivity and virulence of three distinct Klebsiella pneumoniae (hypermucoviscous K1, research K2, carbapenemase-producing ST258) strains utilized, the absence of CD36 significantly increased host susceptibility to acute intrapulmonary infection by K...
September 28, 2016: Journal of Infectious Diseases
A Sorlózano-Puerto, D Esteva-Fernández, J Oteo-Iglesias, J M Navarro-Marí, J Gutiérrez-Fernández
OBJECTIVE: We describe the characterization of a new isolated in Spain of Klebsiella pneumoniae ST258 producing KPC-3, carbapenems non-susceptible, recovered from a sample of urine from a patient with urinary tract infection and no history of carbapenems exposure. METHODS: After the isolation, identification of K. pneumoniae was performed by biochemical tests and mass spectrometry. The carbapenems susceptibility testing was performed by microdilution and E-test in cation-adjusted Mueller-Hinton...
September 2016: Archivos Españoles de Urología
Viola Conte, Monica Monaco, Tommaso Giani, Fortunato D'Ancona, Maria Luisa Moro, Fabio Arena, Marco Maria D'Andrea, Gian Maria Rossolini, Annalisa Pantosti
OBJECTIVES: The spread of carbapenem-resistant Enterobacteriaceae (CRE) represents one of the most worrisome problems for clinical medicine worldwide. In Italy, the Antibiotic-Resistance-Istituto Superiore di Sanità surveillance network, in collaboration with the Committee for Antimicrobial Agents of the Italian Society of Clinical Microbiologists, promoted a study to investigate the carbapenem-resistance mechanisms, clonal relatedness and capsular typing of a recent collection of carbapenem-resistant Klebsiella pneumoniae (CR-KP)...
September 1, 2016: Journal of Antimicrobial Chemotherapy
Inés Bado, Claudia Gutiérrez, Virginia García-Fulgueiras, Nicolás F Cordeiro, Lucía Araújo Pirez, Verónica Seija, Cristina Bazet, Gloria Rieppi, Rafael Vignoli
The objectives of this study were (i) to determine the extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae (ESBL-EcKp) clones circulating in an intensive care unit (ICU) in Uruguay between August 2010 and July 2011, (ii) to characterise the ESBL and plasmid-mediated quinolone resistance (PMQR) genes of the studied isolates and (iii) to determine the virulotype of the clinical isolates. Clinical and gut-colonising ESBL-EcKp from ICU patients were studied. Bacterial identification and antibiotic susceptibility determination were performed using a VITEK(®)2 system...
September 2016: Journal of Global Antimicrobial Resistance
Chang-Ro Lee, Jung Hun Lee, Kwang Seung Park, Young Bae Kim, Byeong Chul Jeong, Sang Hee Lee
The emergence of carbapenem-resistant Gram-negative pathogens poses a serious threat to public health worldwide. In particular, the increasing prevalence of carbapenem-resistant Klebsiella pneumoniae is a major source of concern. K. pneumoniae carbapenemases (KPCs) and carbapenemases of the oxacillinase-48 (OXA-48) type have been reported worldwide. New Delhi metallo-β-lactamase (NDM) carbapenemases were originally identified in Sweden in 2008 and have spread worldwide rapidly. In this review, we summarize the epidemiology of K...
2016: Frontiers in Microbiology
Peter Damborg, Arshnee Moodley, Bent Aalbæk, Gianpiero Ventrella, Teresa Pires Dos Santos, Luca Guardabassi
BACKGROUND: Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged globally in companion animals in the last decade. In Europe, the multidrug-resistant sequence type (ST)71 is widespread, but recently other clones have appeared. The objective of this study was to examine genotypic diversity and antimicrobial resistance of clinical MRSP isolates obtained from dogs, including dogs sampled on multiple occasions, in Denmark over a six-year period. For that purpose a total of 46 clinical MRSP isolates obtained from 36 dogs between 2009 and 2014 were subjected to antimicrobial susceptibility testing, multilocus-sequence typing (MLST) and SCCmec typing...
2016: BMC Veterinary Research
Costas C Papagiannitsis, Vincenzo Di Pilato, Tommaso Giani, Panagiota Giakkoupi, Eleonora Riccobono, Giulia Landini, Vivi Miriagou, Alkiviadis C Vatopoulos, Gian Maria Rossolini
OBJECTIVES: Global dissemination of KPC-type carbapenemases is mainly associated with the spread of high-risk clones of Klebsiella pneumoniae and of KPC-encoding plasmids. In this study, we explored the population structure of KPC-encoding plasmids from the recent epidemics of KPC-producing K. pneumoniae (KPC-Kp) in Greece and Italy, the two major European endemic settings. METHODS: Thirty-four non-replicate clinical strains of KPC-Kp representative of the early phases (2008-11) of the Greek (n = 22) and Italian (n = 12) epidemics were studied...
October 2016: Journal of Antimicrobial Chemotherapy
Qinglan Guo, Adam D Tomich, Christi L McElheny, Vaughn S Cooper, Nicole Stoesser, Minggui Wang, Nicolas Sluis-Cremer, Yohei Doi
OBJECTIVES: The objectives of this study were to elucidate the genetic context of a novel plasmid-mediated fosA variant, fosA6, conferring fosfomycin resistance and to characterize the kinetic properties of FosA6. METHODS: The genome of fosfomycin-resistant Escherichia coli strain YD786 was sequenced. Homologues of FosA6 were identified through BLAST searches. FosA6 and FosA(ST258) were purified and characterized using a steady-state kinetic approach. Inhibition of FosA activity was examined with sodium phosphonoformate...
September 2016: Journal of Antimicrobial Chemotherapy
Martin Kaase, Sven Schimanski, Reinhold Schiller, Bettina Beyreiß, Alexander Thürmer, Jörg Steinmann, Volkhard A Kempf, Christina Hess, Ingo Sobottka, Ines Fenner, Stefan Ziesing, Irene Burckhardt, Lutz von Müller, Axel Hamprecht, Ina Tammer, Nina Wantia, Karsten Becker, Thomas Holzmann, Martina Furitsch, Gabriele Volmer, Sören G Gatermann
Aim of this study was to determine the incidence and molecular epidemiology of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in Germany. E. coli and K. pneumoniae isolates from clinical samples which were non-susceptible to carbapenems were collected in laboratories serving 20 hospitals throughout Germany from November 2013 to April 2014. The isolates were tested for the presence of carbapenemases by PCR and phenotypic methods and typed by multilocus sequence typing. Risk factors including a previous hospitalization abroad were analysed...
September 2016: International Journal of Medical Microbiology: IJMM
Laura Villa, Claudia Feudi, Daniela Fortini, Michele Iacono, Celestino Bonura, Andrea Endimiani, Caterina Mammina, Alessandra Carattoli
Klebsiella pneumoniaesequence type (ST) 307, carryingblaKPC-3,blaCTX-M-15,blaOXA-1,aac(6')-Ib-cr, andqnrB1 genes, is replacing the predominant hyperepidemic ST258 clone in Italy. Whole-genome and complete plasmid sequencing of one ST307 strain was performed and new features were identified.
2016: Genome Announcements
Latania K Logan, Andrea M Hujer, Steven H Marshall, T Nicholas Domitrovic, Susan D Rudin, Xiaotian Zheng, Nadia K Qureshi, Mary K Hayden, Felicia A Scaggs, Anand Karadkhele, Robert A Bonomo
Multidrug-resistant (MDR) Enterobacteriaceae infections are increasing in U.S. children; however, there is a paucity of multicentered analyses of antibiotic resistance genes responsible for MDR phenotypes among pediatric Enterobacteriaceae isolates. In this study, 225 isolates phenotypically identified as extended-spectrum β-lactamase (ESBL) or carbapenemase producers, recovered from children ages 0 to 18 years hospitalized between January 2011 and April 2015 at three Chicago area hospitals, were analyzed...
June 2016: Antimicrobial Agents and Chemotherapy
M J D Dautzenberg, M R Haverkate, M J M Bonten, M C J Bootsma
OBJECTIVES: Observational studies have suggested that Escherichia coli sequence type (ST) 131 and Klebsiella pneumoniae ST258 have hyperendemic properties. This would be obvious from continuously high incidence and/or prevalence of carriage or infection with these bacteria in specific patient populations. Hyperendemicity could result from increased transmissibility, longer duration of infectiousness, and/or higher pathogenic potential as compared with other lineages of the same species...
2016: BMJ Open
Sorabh Dhar, Emily T Martin, Paul R Lephart, John P McRoberts, Teena Chopra, Timothy T Burger, Ruthy Tal-Jasper, Kayoko Hayakawa, Hadas Ofer-Friedman, Tsilia Lazarovitch, Ronit Zaidenstein, Federico Perez, Robert A Bonomo, Keith S Kaye, Dror Marchaim
A "high risk" clone of carbapenem-resistant Klebsiella pneumoniae (CRKP) identified by multilocus sequence typing (MLST) as sequence type (ST) 258 has disseminated worldwide. As the molecular epidemiology of the CRE pandemic continues to evolve, the clinical impact of non-ST258 strains is less well defined. We conducted an epidemiological investigation of CRKP based on strains MLST. Among 68 CRKP patients, 61 were ST258 and 7 belonged to non-ST258. Klebsiella pneumoniae ST258 strains were significantly associated with bla KPC production and with resistance to an increased number of antimicrobials...
January 2016: Open Forum Infectious Diseases
Ana María Ocampo, Carlos Andrés Vargas, Patricia María Sierra, Astrid Vanessa Cienfuegos, Judy Natalia Jiménez
INTRODUCTION: Resistance to carbapenems is considered to represent a serious threat to public health at the global level, since these antibiotics are one of the last therapeutic options for the treatment of multidrug-resistant bacteria. Molecular characterization of outbreaks due to resistant bacteria provides information that can be used in the design of infection control strategies. OBJECTIVE: To describe the molecular characteristics of an outbreak of carbapenem-resistant Klebsiella pneumoniae that occurred in a tertiary care hospital in Medellín in 2010-2011...
December 2015: Biomédica: Revista del Instituto Nacional de Salud
Angeliki Mavroidi, Maria Katsiari, Sofia Likousi, Eleftheria Palla, Zoi Roussou, Charikleia Nikolaou, Asimina Maguina, Evangelia D Platsouka
The emergence of colistin resistance may further contribute to treatment failure of infection caused by multidrug-resistant (MDR) Klebsiella pneumoniae. The colistin resistance rates were determined and colistin-resistant carbapenemase-producing K. pneumoniae (COL-R CP-Kp) were characterized over an 18-month period in a Greek hospital. Out of 135 carbapenemase producers, 19 isolates (14%) were categorized as resistant to colistin. Phenotypic and molecular characterization of the COL-R CP-Kp isolates revealed that all were MDR blaKPC producers and, excluding one isolate of MLST ST383, belonged to the international clonal lineage ST258...
July 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Fabio Arena, Lucia Henrici De Angelis, Antonio Cannatelli, Vincenzo Di Pilato, Marina Amorese, Marco Maria D'Andrea, Tommaso Giani, Gian Maria Rossolini
Using aGalleria mellonellaanimal model, we compared the virulence of two sequence type 258 (ST258) KPC-producingKlebsiella pneumoniaestrains, which were representative of the two clades of this clonal lineage, with that of isogenic colistin-resistantmgrBmutants. With both strains, themgrBmutants did not exhibit modification in virulence. In theG. mellonellamodel, the clade 1 strain (capsular typecps-1[wzi29, producing KPC-2]) was significantly more virulent than the clade 2 strain (capsular typecps-2[wzi154, producing KPC-3])...
April 2016: Antimicrobial Agents and Chemotherapy
E Bathoorn, C Tsioutis, J M da Silva Voorham, E V Scoulica, E Ioannidou, K Zhou, J W Rossen, A Gikas, A W Friedrich, H Grundmann
OBJECTIVES: KPC-2-producing Klebsiella pneumoniae (KPC-KP) ST258 has been rapidly expanding and is often associated with serious nosocomial infections. Last-line antibiotics such as colistin and tigecycline often remain the only treatment option. We describe here the evolving genetic background of KPC-KP isolates in Crete, Greece. METHODS: We tested the antibiotic susceptibility of 34 clinical isolates from patients hospitalized in 2010 and 2013-14. Whole-genome sequences of these isolates were analysed for acquired resistance genes and gene mutations...
May 2016: Journal of Antimicrobial Chemotherapy
Scott D Kobayashi, Adeline R Porter, David W Dorward, Amanda J Brinkworth, Liang Chen, Barry N Kreiswirth, Frank R DeLeo
Carbapenem-resistant Klebsiella pneumoniae strains classified as multilocus sequence type 258 (ST258) are among the most widespread multidrug-resistant hospital-acquired pathogens. Treatment of infections caused by these organisms is difficult, and mortality is high. The basis for the success of ST258, outside of antibiotic resistance, remains incompletely determined. Here we tested the hypothesis that ST258K. pneumoniae has enhanced capacity to circumvent killing by human neutrophils, the primary cellular defense against bacterial infections...
May 15, 2016: Journal of Infectious Diseases
P L Ho, Y Y Cheung, Y Wang, W U Lo, E L Y Lai, K H Chow, V C C Cheng
Carbapenem-resistant Enterobacteriaceae represents a major public health issue. This study investigated the clonality and resistance mechanisms of 92 carbapenem-resistant E. coli (n = 21) and K. pneumoniae (n = 71) isolates collected consecutively from clinical specimens and patients at high risk of carriage between 2010 and 2012 in a healthcare region in Hong Kong. Combined disk tests (CDTs) and the Carba NP test were used for phenotypic detection of carbapenemases. PCR assays were used to detect carbapenemase genes...
March 2016: European Journal of Clinical Microbiology & Infectious Diseases
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