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https://www.readbyqxmd.com/read/29351583/travelers-diarrhea-in-thailand-a-quantitative-analysis-using-taqman%C3%A2-array-card
#1
Paphavee Lertsethtakarn-Ketwalha, Sasikorn Silapong, Pimmada Sakpaisal, Oralak Serichantalergs, Nattaya Ruamsap, Woradee Lurchachaiwong, Sinn Anuras, James A Platts-Mills, Jie Liu, Eric R Houpt, Ladaporn Bodhidatta, Brett E Swierczewski, Carl J Mason
Background: Travelers' diarrhea (TD) is a common illness experienced by travelers from developed countries visiting developing countries. Recent questionnaire-based surveillance studies reported that approximately 6-16% of travelers experienced TD while visiting Thailand; however, a majority of TD information was limited mainly to US military populations. Methods: A TD surveillance study was conducted at Bumrungrad International Hospital in 2012-2014 in Bangkok, Thailand...
January 17, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29351480/mechanistically-different-effects-of-fat-and-sugar-on-insulin-resistance-hypertension-and-gut-microbiota-in-rats
#2
Sara Ramos-Romero, Mercè Hereu, Lidia Atienza, Josefina Casas, Olga Jáuregui, Susana Amézqueta, Gabriel Dasilva, Isabel Medina, Maria Rosa Nogués, Marta Romeu, Josep Lluís Torres
Insulin resistance (IR) and impaired glucose tolerance (IGT) are the first manifestations of diet-induced metabolic alterations leading to type-2 diabetes, while hypertension is the deadliest risk factor of cardiovascular disease. The roles of dietary fat and fructose in the development of IR, IGT and hypertension are controversial. We tested the long-term effects of an excess of fat or sucrose (fructose/glucose) on healthy male Wistar Kyoto (WKY) rats. Fat affects IR and IGT earlier than fructose through low-grade systemic inflammation evidenced by liver inflammatory infiltration, increased levels of plasma interleukin-6, prostaglandin E2 and reduced levels of protective short-chain fatty acids without triggering hypertension...
January 2, 2018: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29351316/dexmedetomidine-reduces-lipopolysaccharide-induced-neuroinflammation-sickness-behavior-and-anhedonia
#3
Ching-Hua Yeh, Liang-Po Hsieh, Ming-Chung Lin, Tsui-Shan Wei, Hui-Ching Lin, Chia-Cheng Chang, Chung-Hsi Hsing
BACKGROUND: Peripheral innate immune response may induce sickness behavior through activating microglia, excessive cytokines production, and neuroinflammation. Dexmedetomidine (Dex) has anti-inflammatory effect. We investigated the effects of Dex on lipopolysaccharide (LPS)-induced neuroinflammation and sickness behavior in mice. MATERIALS AND METHODS: BALB/c mice were intraperitoneally (i.p.) injected with Dex (50 ug/kg) or vehicle. One hour later, the mice were injected (i...
2018: PloS One
https://www.readbyqxmd.com/read/29351274/dna-polymerase-iv-primarily-operates-outside-of-dna-replication-forks-in-escherichia-coli
#4
Sarah S Henrikus, Elizabeth A Wood, John P McDonald, Michael M Cox, Roger Woodgate, Myron F Goodman, Antoine M van Oijen, Andrew Robinson
In Escherichia coli, damage to the chromosomal DNA induces the SOS response, setting in motion a series of different DNA repair and damage tolerance pathways. DNA polymerase IV (pol IV) is one of three specialised DNA polymerases called into action during the SOS response to help cells tolerate certain types of DNA damage. The canonical view in the field is that pol IV primarily acts at replisomes that have stalled on the damaged DNA template. However, the results of several studies indicate that pol IV also acts on other substrates, including single-stranded DNA gaps left behind replisomes that re-initiate replication downstream of a lesion, stalled transcription complexes and recombination intermediates...
January 19, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29351264/identification-of-cyclic-dipeptides-from-escherichia-coli-as-new-antimicrobial-agents-against-ralstonia-solanacearum
#5
Shihao Song, Shuna Fu, Xiuyun Sun, Peng Li, Ji'en Wu, Tingyan Dong, Fei He, Yinyue Deng
Ralstonia solanacearum is a causative agent of bacterial wilt in many important crops throughout the world. How to control bacterial wilt caused by R. solanacearum is a major problem in agriculture. In this study, we aim to isolate the biocontrol agents that have high efficacy in the control of bacterial wilt. Three new bacterial strains with high antimicrobial activity against R. solanacearum GMI1000 were isolated and identified. Our results demonstrated that these bacteria could remarkably inhibit the disease index of host plant infected by R...
January 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29351236/trends-in-resistance-to-extended-spectrum-cephalosporins-and-carbapenems-among-escherichia-coli-and-klebsiella-spp-isolates-in-a-district-in-western-india-during-2004-2014
#6
Ingvild Odsbu, Smita Khedkar, Frida Lind, Uday Khedkar, Sandeep S Nerkar, Nicola Orsini, Ashok J Tamhankar, Cecilia Stålsby Lundborg
Surveillance data on the level of resistant bacteria is needed to inform strategies to reduce the development and spread of antibiotic resistance. The aim of this study was to determine the non-susceptibility trends to extended-spectrum cephalosporins and carbapenems among Escherichia coli and Klebsiella spp. isolates from the district of Nashik in Western India during the period 2004-2014. Antibacterial susceptibility testing of clinical isolates was performed using Kirby-Bauer disc diffusion method to determine inhibitory zone diameters...
January 19, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29350905/ampulexins-a-new-family-of-peptides-in-venom-of-the-emerald-jewel-wasp-ampulex-compressa
#7
Eugene Moore, Ryan Arvidson, Christopher Banks, Jean Urenda, Elizabeth Duong, Haroun Mohammad, Michael E Adams
The parasitoid wasp Ampulex compressa injects venom directly into the brain and subesophageal ganglion of the cockroach Periplaneta americana, inducing a seven to ten day lethargy termed hypokinesia. Hypokinesia presents as a significant reduction in both escape response and spontaneous walking. We examined aminergic and peptidergic components of milked venom with HPLC and MALDI-TOF mass spectrometry. HPLC coupled with electrochemical detection confirmed presence of dopamine in milked venom, while mass spectrometry revealed that the venom gland and venom sac have distinct peptide profiles, with milked venom predominantly composed of venom sac peptides...
January 19, 2018: Biochemistry
https://www.readbyqxmd.com/read/29350898/identification-of-individual-bacterial-cells-through-the-intermolecular-interactions-with-peptide-functionalized-solid-state-pores
#8
Makusu Tsutsui, Masayoshi Tanaka, Takahiro Marui, Kazumichi Yokota, Takeshi Yoshida, Akihide Arima, Wataru Tonomura, Masateru Taniguchi, Takashi Washio, Mina Okochi, Tomoji Kawai
Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between the TLR5-derived recognition peptides and flagella induce ligand-specific perturbations in the translocation dynamics of Escherichia coli, which facilitated the discrimination between the wild-type and flagellin-deletion mutant (ΔfliC) by the resistive pulse patterns thereby demonstrating the sensing of bacteria at a single-cell level...
January 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29350441/ultrasensitive-detection-of-shiga-toxin-2-and-its-variants-in-shiga-toxin-producing-escherichia-coli-strains-by-a-time-resolved-fluorescence-immunoassay
#9
Tian Wen, Chao Huang, Yi Zhang, Xiaoyan Zeng, Wendong Liu, Zhenbang Jiao, Xiling Guo, Yongjun Jiao
A rapid and sensitive two-step time-resolved fluorescence immunoassay (TRFIA) was developed for the detection of Shiga toxin 2 (Stx2) and its variants in Shiga toxin-producing Escherichia coli (STEC) strains. In sandwich mode, a monoclonal antibody against Stx2 was coated on a microtiter plate as a capture antibody. A tracer antibody against Stx2 labeled with europium(III) (Eu3+ ) chelate was then used as a detector, followed by fluorescence measurements using time-resolved fluorescence. The sensitivity of Stx2 detection was 0...
January 19, 2018: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/29350317/a-study-on-the-role-that-quorum-sensing-play-in-antibiotic-resistant-plasmid-conjugative-transfer-in-escherichia-coli
#10
Yueheng Zhang, Qingping Ma, Bingmei Su, Rui Chen, Juan Lin, Zhifen Lin, Dali Wang, Yang Yu
Horizontal genes transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes (ARGs) in the environment. However, the mechanisms of HGT of ARGs under the influence of antibiotics in sub-MIC remain rarely explored. Moreover, given its collective nature, HGT was considered to be relative to quorum sensing (QS) system. To investigate whether QS has any impact on horizontal gene transfer of ARGs, experiments were conducted to determine the conjugative efficiency of plasmid RP4 on Escherichia coli (E...
January 19, 2018: Ecotoxicology
https://www.readbyqxmd.com/read/29350071/disclosing-respiratory-coinfections-a-broad-range-panel-assay-for-avian-respiratory-pathogens-on-a-nanofluidic-pcr-platform
#11
Guillaume Croville, Charlotte Foret, Pauline Heuillard, Alexis Senet, Mattias Delpont, Mohammed Mouahid, Mariette F Ducatez, Faouzi Kichou, Jean-Luc Guerin
Respiratory syndromes (RS) are among the most significant pathological conditions in food animals and are caused by complex coactions of pathogens and environmental factors. In poultry, low pathogenic avian Influenza A viruses, metapneumoviruses, infectious bronchitis virus, infectious laryngotracheitis virus, Mycoplasma spp. Escherichia coli and/or Ornithobacterium rhinotracheale (ORT) in turkeys, are considered as key co-infectious agents of respiratory syndromes. Aspergillus sp., Pasteurella multocida, Avibacterium paragallinarum or Chlamydia psittaci may also be involved in respiratory outbreaks...
January 19, 2018: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/29350038/efficient-mass-spectral-analysis-of-active-transporters-overexpressed-in-escherichia-coli
#12
Mamiyo Kawakami, Narinobu Juge, Yuri Kato, Hiroshi Omote, Yoshinori Moriyama, Takaaki Miyaji
Structural analysis of purified active membrane proteins can be performed by mass spectrometry (MS). However, no large-scale expression systems for active eukaryotic membrane proteins are available. Moreover, because membrane proteins cannot easily be digested by trypsin and ionized, they are difficult to analyze by MS. Here, we developed a method for mass spectral analysis of eukaryotic membrane proteins combined to an overexpression system in Escherichia coli. Vesicular glutamate transporter 2 (VGLUT2/SLC17A6) with a soluble α-helical protein and histidine tag on the N- and C-terminus, respectively, was overexpressed in E...
January 19, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29349940/etiology-of-invasive-bacterial-infections-in-immunocompetent-children-in-korea-2006-2010-a-retrospective-multicenter-study
#13
Kyuyol Rhie, Eun Hwa Choi, Eun Young Cho, Jina Lee, Jin Han Kang, Dong Soo Kim, Yae Jean Kim, Youngmin Ahn, Byung Wook Eun, Sung Hee Oh, Sung Ho Cha, Young Jin Hong, Kwang Nam Kim, Nam Hee Kim, Yun Kyung Kim, Jong Hyun Kim, Taekjin Lee, Hwang Min Kim, Kun Song Lee, Chun Soo Kim, Su Eun Park, Young Mi Kim, Chi Eun Oh, Sang Hyuk Ma, Dae Sun Jo, Young Youn Choi, Hoan Jong Lee
BACKGROUND: Invasive bacterial infections in apparently immunocompetent children were retrospectively analyzed to figure causative bacterial organisms in Korea. METHODS: A total of 947 cases from 25 university hospitals were identified from 2006 to 2010 as a continuance of a previous 10-year period study from 1996 to 2005. RESULTS: Escherichia coli (41.3%), Streptococcus agalactiae (27.7%), and Staphylococcus aureus (27.1%) were the most common pathogens in infants < 3 months of age...
February 5, 2018: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/29349625/enhancement-of-the-thermal-and-alkaline-ph-stability-of-escherichia-coli-lysine-decarboxylase-for-efficient-cadaverine-production
#14
Fengyu Kou, Jing Zhao, Jiao Liu, Cunmin Sun, Yanmei Guo, Zijian Tan, Feng Cheng, Zhimin Li, Ping Zheng, Jibin Sun
OBJECTIVE: To enhance the thermal and alkaline pH stability of the lysine decarboxylase from Escherichia coli (CadA) by engineering the decameric interface and explore its potential for industrial applications. RESULTS: The mutant T88S was designed for improved structural stability by computational analysis. The optimal pH and temperature of T88S were 7.0 and 55 °C (5.5 and 50 °C for wild-type). T88S showed higher thermostability with a 2.9-fold increase in the half-life at 70 °C (from 11 to 32 min) and increased melting temperature (from 76 to 78 °C)...
January 18, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29349568/class-iii-bacteriocin-helveticin-m-causes-sublethal-damage-on-target-cells-through-impairment-of-cell-wall-and-membrane
#15
Zhilan Sun, Xiaomeng Wang, Xinxiao Zhang, Haihong Wu, Ye Zou, Pengpeng Li, Chong Sun, Weimin Xu, Fang Liu, Daoying Wang
Helveticin-M, a novel Class III bacteriocin produced by Lactobacillus crispatus exhibited an antimicrobial activity against Staphylococcus aureus, S. saprophyticus, and Enterobacter cloacae. To understand how Helveticin-M injured target cells, Helveticin-M was cloned and heterologously expressed in Escherichia coli. Subsequently, the cell wall organization and cell membrane integrity of target cells were determined. The mechanism of cellular damage differed according to bacterial species. Based on morphology analysis, Helveticin-M disrupted the cell wall of Gram-positive bacteria and disorganized the outer membrane of Gram-negative bacteria, therefore, altering surface structure...
January 18, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29349492/ganoderma-lucidum-phosphoglucomutase-is-required-for-hyphal-growth-polysaccharide-production-and-cell-wall-integrity
#16
Yanru Hu, Mengjiao Li, Shengli Wang, Sining Yue, Liang Shi, Ang Ren, Mingwen Zhao
Phosphoglucomutase (pgm) is an important enzyme in carbohydrate metabolism that is located at the branching point between glycolysis and the Leloir pathway. pgm catalyzes the reversible conversion reaction between glucose-6-phosphate (Glc-6-P) and glucose-1-phosphate (Glc-1-P). The glpgm gene was cloned in Escherichia coli, and the recombinant pgm protein from Ganoderma lucidum was purified in this study. The activity of native pgm was also detected to demonstrate that this predicted gene was functional in G...
January 18, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29349039/engineering-escherichia-coli-for-the-production-of-terpene-mixture-enriched-in-caryophyllene-and-caryophyllene-alcohol-as-potential-aviation-fuel-compounds
#17
Weihua Wu, Fang Liu, Ryan W Davis
Recent studies have revealed that caryophyllene and its stereoisomers not only exhibit multiple biological activities but also have desired properties as renewable candidates for ground transportation and jet fuel applications. This study presents the first significant production of caryophyllene and caryolan-1-ol by an engineered E. coli with heterologous expression of mevalonate pathway genes with a caryophyllene synthase and a caryolan-1-ol synthase. By optimizing metabolic flux and fermentation parameters, the engineered strains yielded 449 mg/L of total terpene, including 406 mg/L sesquiterpene with 100 mg/L caryophyllene and 10 mg/L caryolan-1-ol...
June 2018: Metabolic Engineering Communications
https://www.readbyqxmd.com/read/29348819/recombinant-protein-of-haemonchus-contortus-small-gtpase-adp-ribosylation-factor-1-hcarf1-modulate-the-cell-mediated-immune-response-in-vitro
#18
Javaid Ali Gadahi, Muhammad Ehsan, Shuai Wang, Zhenchao Zhang, Ruofeng Yan, Xiaokai Song, Lixin Xu, Xiangrui Li
ADP-ribosylation factors (ARFs) are members of the Ras-related small GTPase family involved in the vesicular trafficking regulation. Immunomodulatory effects of these proteinson host cell arenot being addressed yet. H. contortus small GTPase ADP-ribosylation 1 gene (HcARF1) was cloned and recombinant protein of HcARF1 (rHcARF1) was successfully expressed in Escherichia coli. Binding activity of rHcARF1 to goat PBMCs was confirmed by immunofluorescence assay (IFA) and its immunomudulatory effects on cytokine secretion, cell proliferation, cell migration and nitric oxide production (NO) were observed by co-incubation of rHcARF1...
December 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29348586/analogues-of-disulfides-from-allium-stipitatum-demonstrate-potent-anti-tubercular-activities-through-drug-efflux-pump-and-biofilm-inhibition
#19
Cynthia A Danquah, Eleftheria Kakagianni, Proma Khondkar, Arundhati Maitra, Mukhlesur Rahman, Dimitrios Evangelopoulos, Timothy D McHugh, Paul Stapleton, John Malkinson, Sanjib Bhakta, Simon Gibbons
Disulfides from Allium stipitatum, commonly known as Persian shallot, were previously reported to possess antibacterial properties. Analogues of these compounds, produced by S-methylthiolation of appropriate thiols using S-methyl methanethiosulfonate, exhibited antimicrobial activity, with one compound inhibiting the growth of Mycobacterium tuberculosis at 17 µM (4 mg L-1) and other compounds inhibiting Escherichia coli and multi-drug-resistant (MDR) Staphylococcus aureus at concentrations ranging between 32-138 µM (8-32 mg L-1)...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29348433/speciation-of-common-gram-negative-pathogens-using-a-highly-multiplexed-high-resolution-melt-curve-assay
#20
Thomas Edwards, Shugo Sasaki, Christopher Williams, Glyn Hobbs, Nicholas A Feasey, Katie Evans, Emily R Adams
The identification of the bacterial species responsible for an infection remains an important step for the selection of antimicrobial therapy. Gram-negative bacteria are an important source of hospital and community acquired infections and frequently antimicrobial resistant. Speciation of bacteria is typically carried out by biochemical profiling of organisms isolated from clinical specimens, which is time consuming and delays the initiation of tailored treatment. Whilst molecular methods such as PCR have been used, they often struggle with the challenge of detecting and discriminating a wide range of targets...
January 18, 2018: Scientific Reports
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