keyword
https://read.qxmd.com/read/37566362/psidium-guajava-l-phenolic-compound-reinforced-lamellar-scaffold-for-tracheal-tissue-engineering
#21
JOURNAL ARTICLE
Venâncio A Amaral, Juliana Ferreira de Souza, Thais F R Alves, José M de Oliveira Junior, Patrícia Severino, Norberto Aranha, Eliana B Souto, Marco V Chaud
The aim of this work was to develop a dense lamellar scaffold, as a biomimetic material with potential applications in the regeneration of tracheal tissue after surgical tumor resection. The scaffolds were produced by plastic compression technique, exploiting the use of total phenolic compounds (TPC) from Psidium guajava Linn as a potential cross-linking agent in a polymeric mixture based on collagen (COL), silk fibroin (SF), and polyethylene glycol 400 (PEG 400). Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) confirmed the chemical interactions between the polymers and the cross-linking of TPC between COL and SF...
August 11, 2023: Drug Delivery and Translational Research
https://read.qxmd.com/read/37476057/biomimetic-in-situ-tracheal-microvascularization-for-segmental-tracheal-reconstruction-in-one-step
#22
JOURNAL ARTICLE
Fei Sun, Zhiming Shen, Boyou Zhang, Yi Lu, Yibo Shan, Qiang Wu, Lei Yuan, Jianwei Zhu, Shu Pan, Zhihao Wang, Cong Wu, Guozhong Zhang, Wenlong Yang, Xiangyu Xu, Hongcan Shi
Formation of functional and perfusable vascular network is critical to ensure the long-term survival and functionality of the engineered tissue tracheae after transplantation. However, the greatest challenge in tracheal-replacement therapy is the promotion of tissue regeneration by rapid graft vascularization. Traditional prevascularization methods for tracheal grafts typically utilize omentum or muscle flap wrapping, which requires a second operation; vascularized segment tracheal orthotopic transplantation in one step remains difficult...
July 2023: Bioengineering & Translational Medicine
https://read.qxmd.com/read/37438368/regeneration-of-tracheal-neotissue-in-partially-decellularized-scaffolds
#23
JOURNAL ARTICLE
Zheng Hong Tan, Sayali Dharmadhikari, Lumei Liu, Jane Yu, Kimberly M Shontz, Jacob T Stack, Christopher K Breuer, Susan D Reynolds, Tendy Chiang
Extensive tracheal injury or disease can be life-threatening but there is currently no standard of care. Regenerative medicine offers a potential solution to long-segment tracheal defects through the creation of scaffolds that support the generation of healthy neotissue. We developed decellularized tracheal grafts (PDTG) by removing the cells of the epithelium and lamina propria while preserving donor cartilage. We previously demonstrated that PDTG support regeneration of host-derived neotissue. Here, we use a combination of microsurgical, immunofluorescent, and transcriptomic approaches to compare PDTG neotissue with the native airway and surgical controls...
July 12, 2023: NPJ Regenerative Medicine
https://read.qxmd.com/read/37371697/articular-cartilage-regeneration-by-hyaline-chondrocytes-a-case-study-in-equine-model-and-outcomes
#24
JOURNAL ARTICLE
Fernando Canonici, Cristiano Cocumelli, Antonella Cersini, Daniele Marcoccia, Alessia Zepparoni, Annalisa Altigeri, Daniela Caciolo, Cristina Roncoroni, Valentina Monteleone, Elisa Innocenzi, Cristian Alimonti, Paola Ghisellini, Cristina Rando, Eugenia Pechkova, Roberto Eggenhöffner, Maria Teresa Scicluna, Katia Barbaro
Cartilage injury defects in animals and humans result in the development of osteoarthritis and the progression of joint deterioration. Cell isolation from equine hyaline cartilage and evaluation of their ability to repair equine joint cartilage injuries establish a new experimental protocol for an alternative approach to osteochondral lesions treatment. Chondrocytes (CCs), isolated from the autologous cartilage of the trachea, grown in the laboratory, and subsequently arthroscopically implanted into the lesion site, were used to regenerate a chondral lesion of the carpal joint of a horse...
May 31, 2023: Biomedicines
https://read.qxmd.com/read/37365963/long-term-chondrocyte-retention-in-partially-decellularized-tracheal-grafts
#25
JOURNAL ARTICLE
Maxwell Bergman, Jacqueline Harwood, Lumei Liu, Kimberly M Shontz, Coreena Chan, Tendy Chiang
OBJECTIVE: Decellularized tracheal grafts possess the biological cues necessary for tissue regeneration. However, conventional decellularization approaches to target the removal of all cell populations including chondrocytes lead to a loss of mechanical support. We have created a partially decellularized tracheal graft (PDTG) that preserves donor chondrocytes and the mechanical properties of the trachea. In this study, we measured PDTG chondrocyte retention with a murine microsurgical model...
June 27, 2023: Otolaryngology—Head and Neck Surgery
https://read.qxmd.com/read/37346796/development-of-tissue-engineered-tracheal-scaffold-with-refined-mechanical-properties-and-vascularisation-for-tracheal-regeneration
#26
JOURNAL ARTICLE
Tehreem Khalid, Luis Soriano, Mark Lemoine, Sally-Ann Cryan, Fergal J O'Brien, Cian O'Leary
Introduction: Attempted tracheal replacement efforts thus far have had very little success. Major limiting factors have been the inability to efficiently re-vascularise and mimic the mechanical properties of native tissue. The major objective of this study was to optimise a previously developed collagen-hyaluronic acid scaffold (CHyA-B), which has shown to facilitate the growth of respiratory cells in distinct regions, as a potential tracheal replacement device. Methods: A biodegradable thermoplastic polymer was 3D-printed into different designs and underwent multi-modal mechanical assessment...
2023: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/37334440/quasi-dynamic-breathing-model-of-the-lung-incorporating-viscoelasticity-of-the-lung-tissue
#27
JOURNAL ARTICLE
Nitin Daphalapurkar, Jacob Riglin, Arvind Mohan, Jennifer Harris, John Bernardin
We advanced a novel model to calculate viscoelastic lung compliance and airflow resistance in presence of mucus, accounting for the quasi-linear viscoelastic stress-strain response of the parenchyma (alveoli) tissue. We adapted a continuum-based numerical modeling approach for the lung, integrating the fluid mechanics of the airflow within individual generations of the bronchi and alveoli. The model accounts for elasticity of the deformable bronchioles, resistance to airflow due to the presence of mucus within the bronchioles, and subsequent mucus flow...
June 18, 2023: International Journal for Numerical Methods in Biomedical Engineering
https://read.qxmd.com/read/37313949/optimization-of-chondrocyte-viability-in-partially-decellularized-tracheal-grafts
#28
JOURNAL ARTICLE
Maxwell Bergman, Jacqueline Harwood, Lumei Liu, Sayali Dharmadikhari, Kimberly M Shontz, Tendy Chiang
OBJECTIVE: Advancements in tissue-engineered tracheal replacement (TETR) show promise for the use of partially decellularized tracheal grafts (PDTG) to address critical gaps in airway management and reconstruction. In this study, aiming to leverage the immunoprivileged nature of cartilage to preserve tracheal biomechanics, we optimize PDTG for retention of native chondrocytes. STUDY DESIGN: Comparison in vivo murine study. SETTING: Research Institute affiliated with Tertiary Pediatric Hospital...
June 14, 2023: Otolaryngology—Head and Neck Surgery
https://read.qxmd.com/read/37285021/incompletely-decellularized-tracheal-matrix-scaffold-for-tissue-engineering
#29
JOURNAL ARTICLE
Yu Zhou, Jianhua Zhang, Mengqing Zang
BACKGROUND: Dense cartilaginous extracellular matrix makes decellularization and repopulation of tracheal cartilage difficult. However, the dense matrix isolates cartilaginous antigens from the recipient's immune system. Therefore, allorejection may be avoided by removing antigens from non-cartilaginous tissues. In this study, incompletely decellularized tracheal matrix scaffolds were developed for tracheal tissue engineering. METHODS: Brown Norway rat tracheae were decellularized with 4% sodium deoxycholate treatment...
May 31, 2023: Plastic and Reconstructive Surgery
https://read.qxmd.com/read/37268515/laryngotracheal-reconstruction-for-subglottic-and-tracheal-stenosis
#30
REVIEW
Mollie C Perryman, Shannon M Kraft, Hannah L Kavookjian
Laryngotracheal stenosis is the common endpoint for any process that results in the narrowing of the airway at the level of the glottis, subglottis, or trachea. Although endoscopic procedures are effective in opening the airway lumen, open resection and reconstruction can be necessary to reconstitute a functional airway. When resection and anastomosis are insufficient due to extensive length or location of the stenosis, autologous grafts can be used to expand the airway. Future directions in airway reconstruction include tissue engineering and allotransplantation...
August 2023: Otolaryngologic Clinics of North America
https://read.qxmd.com/read/37214293/pre-epithelialized-cryopreserved-tracheal-allograft-for-neo-trachea-flap-engineering
#31
JOURNAL ARTICLE
Ning Zeng, Youbai Chen, Yewen Wu, Mengqing Zang, Rene D Largo, Edward I Chang, Mark V Schaverien, Peirong Yu, Qixu Zhang
Background: Tracheal reconstruction presents a challenge because of the difficulty in maintaining the rigidity of the trachea to ensure an open lumen and in achieving an intact luminal lining that secretes mucus to protect against infection. Methods: On the basis of the finding that tracheal cartilage has immune privilege, researchers recently started subjecting tracheal allografts to "partial decellularization" (in which only the epithelium and its antigenicity are removed), rather than complete decellularization, to maintain the tracheal cartilage as an ideal scaffold for tracheal tissue engineering and reconstruction...
2023: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/37195529/engineering-large-airways
#32
JOURNAL ARTICLE
Tehreem Khalid, Cian O'Leary
A key issue facing trachea replacement attempts has been the discrepancy of the mechanical properties between the native tracheal tissue and that of the replacement construct; this difference is often one of the major causes for implant failure in vivo and within clinical efforts. The trachea is composed of distinct structural regions, with each component fulfilling a different role in maintaining overall tracheal stability. The trachea's horseshoe-shaped hyaline cartilage rings, smooth muscle and annular ligament collectively produce an anisotropic tissue that allows for longitudinal extensibility and lateral rigidity...
2023: Advances in Experimental Medicine and Biology
https://read.qxmd.com/read/37171579/comparison-of-the-biological-properties-between-3d-printed-and-decellularized-tracheal-grafts
#33
JOURNAL ARTICLE
Yao Wang, Jianfeng Li, Jun Qian, Yunhao Sun, Jianning Xu, Jian Sun
This study sought to characterize the differences between the 3D-printed and decellularized tracheal grafts, providing the basis for the synthesis of the more reasonable and effective tissue-engineered trachea. We compared the biomechanical properties and biocompatibility of the 3D-printed tracheal graft and decellularized tracheal graft in vitro and evaluated the biocompatibility, immune rejection and inflammation of the two materials through in vivo implantation experiments. Compared with the decellularized tracheal graft, the 3D-printed tracheal graft was associated with obviously higher biomechanical properties...
May 12, 2023: Bioprocess and Biosystems Engineering
https://read.qxmd.com/read/37171223/current-status-and-future-trends-of-reconstructing-a-vascularized-tissue-engineered-trachea
#34
REVIEW
Ziqing Shen, Tian Xia, Jun Zhao, Shu Pan
Alternative treatment of long tracheal defects remains one of the challenges faced by thoracic surgeons. Tissue engineering has shown great potential in addressing this regenerative medicine conundrum and the technology to make tracheal grafts using this technique is rapidly maturing, leading to unique therapeutic approaches. However, the clinical application of tissue-engineered tracheal implants is limited by insufficient revascularization. Among them, realizing the vascularization of a tissue-engineered trachea is the most challenging problem to overcome...
May 12, 2023: Connective Tissue Research
https://read.qxmd.com/read/37125371/novel-therapy-for-acquired-tracheomalacia-with-a-tissue-engineered-extraluminal-tracheal-splint-and-autologous-mesenchymal-derived-chondrocytes
#35
JOURNAL ARTICLE
Yaaziel Melgarejo-Ramírez, Juan C Ceballos-Cantú, Eric de J Montes-Olave, Bruno Hernández-Tapia, Víctor Rojas-Figueroa, Gabriela Ramírez-Arroyo, Fabiana Cortés-Acosta, Mario Dorantes-Pavón, Hugo Lecona-Butrón, Olga E Beltrán-Rodríguez-Cabo
Introduction  Acquired tracheomalacia (ATM) is characterized by a loss of structural strength of the tracheal framework, resulting in airway collapse during breathing. Near half of the patients undergoing prolonged invasive mechanical ventilation will suffer tracheal lesions. Treatment for ATM includes external splinting with rib grafts, prosthetic materials, and tracheal resection. Failure in the use of prosthetic materials has made reconsidering natural origin scaffolds and tissue engineering as a suitable alternative...
April 2023: International Archives of Otorhinolaryngology
https://read.qxmd.com/read/37036314/successful-early-neovascularization-in-composite-tracheal-grafts
#36
JOURNAL ARTICLE
Sarah C Nyirjesy, Jane Yu, Sayali Dharmadhikari, Lumei Liu, Maxwell Bergman, Zheng Hong Tan, Kyle K VanKoevering, Tendy Chiang
OBJECTIVE: Long-segment tracheal defects require tissue replacement for successful reconstruction. Rapid revascularization is imperative to maintain graft function. We previously showed that partially decellularized tracheal grafts (PDTG) and composite tracheal grafts (CTG; PDTG supported by a 3-dimensionally printed external splint) regenerate respiratory epithelium and may support the regeneration of endothelial cells (CD31+). However, the capability of graft endothelial cells to organize or contribute to tracheal revascularization remains unclear...
April 10, 2023: Otolaryngology—Head and Neck Surgery
https://read.qxmd.com/read/36980229/development-and-in-vitro-in-vivo-comparative-characterization-of-cryopreserved-and-decellularized-tracheal-grafts
#37
JOURNAL ARTICLE
Elena Stocco, Silvia Barbon, Marco Mammana, Diletta Trojan, Alice Bianchin, Francesca Favaretto, Martina Contran, Giovanni Zambello, Andrea Vogliardi, Marta Confalonieri, Silvia Todros, Piero G Pavan, Filippo Romanato, Maria Teresa Conconi, Veronica Macchi, Raffaele De Caro, Federico Rea, Andrea Porzionato
Tracheal reconstruction represents a challenge when primary anastomosis is not feasible. Within this scenario, the study aim was to develop a new pig-derived decellularized trachea (DecellT) to be compared with the cryopreserved counterpart (CryoT) for a close predictive analysis. Tracheal segments underwent decellularization by a physical + enzymatic + chemical method (12 cycles); in parallel, cryopreserved samples were also prepared. Once decellularized (histology/DNA quantification), the two groups were characterized for Alpha-Gal epitopes/structural proteins (immunohistochemistry/histology/biochemical assays/second harmonic generation microscopy)/ultrastructure (Scanning Electron Microscopy (SEM))/mechanical behaviour...
March 13, 2023: Cells
https://read.qxmd.com/read/36967724/decellularization-compromises-mechanical-and-structural-properties-of-the-native-trachea
#38
JOURNAL ARTICLE
Allison M Greaney, Abhay B Ramachandra, Yifan Yuan, Arina Korneva, Jay D Humphrey, Laura E Niklason
Tracheal replacement using tissue engineering technologies offers great potential to improve previously intractable clinical interventions, and interest in this area has increased in recent years. Many engineered airway constructs currently rely on decellularized native tracheas to serve as the scaffold for tissue repair. Yet, mechanical failure leading to airway narrowing and collapse remains a major cause of morbidity and mortality following clinical implantation of decellularized tracheal grafts. To understand better the factors contributing to mechanical failure in vivo , we characterized the histo-mechanical properties of tracheas following two different decellularization protocols, including one that has been used clinically...
March 2023: Biomater Biosyst
https://read.qxmd.com/read/36874984/preclinical-and-clinical-orthotopic-transplantation-of-decellularized-engineered-tracheal-scaffolds-a-systematic-literature-review
#39
REVIEW
Elena Stocco, Silvia Barbon, Marco Mammana, Giovanni Zambello, Martina Contran, Pier Paolo Parnigotto, Veronica Macchi, Maria Teresa Conconi, Federico Rea, Raffaele De Caro, Andrea Porzionato
Severe tracheal injuries that cannot be managed by mobilization and end-to-end anastomosis represent an unmet clinical need and an urgent challenge to face in surgical practice; within this scenario, decellularized scaffolds (eventually bioengineered) are currently a tempting option among tissue engineered substitutes. The success of a decellularized trachea is expression of a balanced approach in cells removal while preserving the extracellular matrix (ECM) architecture/mechanical properties. Revising the literature, many Authors report about different methods for acellular tracheal ECMs development; however, only few of them verified the devices effectiveness by an orthotopic implant in animal models of disease...
2023: Journal of Tissue Engineering
https://read.qxmd.com/read/36792845/toward-a-robust-swallowing-detection-for-an-implantable-active-artificial-larynx-a-survey
#40
REVIEW
Adrien Mialland, Ihab Atallah, Agnès Bonvilain
Total laryngectomy consists in the removal of the larynx and is intended as a curative treatment for laryngeal cancer, but it leaves the patient with no possibility to breathe, talk, and swallow normally anymore. A tracheostomy is created to restore breathing through the throat, but the aero-digestive tracts are permanently separated and the air no longer passes through the nasal tracts, which allowed filtration, warming, humidification, olfaction, and acceleration of the air for better tissue oxygenation. As for phonation restoration, various techniques allow the patient to talk again...
February 16, 2023: Medical & Biological Engineering & Computing
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