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Quorum sensing

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https://www.readbyqxmd.com/read/29334092/synthetic-cells-produce-a-quorum-sensing-chemical-signal-perceived-by-pseudomonas-aeruginosa
#1
Giordano Rampioni, Francesca D'Angelo, Marco Messina, Alessandro Zennaro, Yutetsu Kuruma, Daniela Tofani, Livia Leoni, Pasquale Stano
Recent developments in bottom-up synthetic biology (e.g., lipid vesicle technology integrated with cell-free protein expression systems) allow the generation of semi-synthetic minimal cells (in short, synthetic cells, SCs) endowed with some distinctive capacities of natural cells. In particular, such approaches provide technological tools and conceptual frameworks for the design and engineering of programmable SCs capable of communicating with natural cells by exchanging chemical signals. Here we describe the generation of giant vesicle-based SCs which, via gene expression, synthesize in their aqueous lumen an enzyme that in turn produces a chemical signal...
January 15, 2018: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29325130/emergent-heterogeneous-micro-environments-in-biofilms-substratum-surface-heterogeneity-and-bacterial-adhesion-force-sensing
#2
Yijin Ren, Can Wang, Zhi Chen, Elaine Allan, Henny C van der Mei, Henk J Busscher
Phenotypically-heterogeneous micro-environments emerge as biofilms mature across different environments. Phenotypic-heterogeneity in biofilm sub-populations not obeying quorum sensing-dictated, collective group-behavior, may be considered as a strategy allowing non-conformists to survive hostile conditions. Heterogeneous phenotype development has been amply studied with respect to gene expression and genotypic changes, but 'biofilm genes' responsible for pre-programmed development of heterogeneous micro-environments in biofilms have never been discovered...
January 9, 2018: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/29324344/anti-biofilm-effect-of-novel-thiazole-acid-analogs-against-pseudomonas-aeruginosa-through-iqs-pathways
#3
Shengrong Li, Siyu Chen, Jilin Fan, Zhen Cao, Weihao Ouyang, Ning Tong, Xin Hu, Jie Hu, Peishan Li, Zifeng Feng, Xi Huang, Yuying Li, Mingshan Xie, Ruikun He, Jingyi Jian, Biyuan Wu, Chen Xu, Weijian Wu, Jialiang Guo, Jing Lin, Pinghua Sun
IQS has been proven to be a new quorum sensing (QS) system against bacterial biofilm formation, which is activated in the common phosphate-limiting environment of infected tissues taking over the central las system. Up to now, numerous biofilm inhibitors which function by affecting traditional QS system have been reported. However, no compound has been reported to exert anti-biofilm activity through IQS system. Herein, various novel IQS derivatives were synthesized by the reaction of thiazole-4-carboxylic acid with different linear alcohols (R-OH) or amines (R-NH2)...
December 27, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29322563/microfluidics-based-lc-ms-mrm-approach-for-the-relative-quantification-of-burkholderia-cenocepacia-secreted-virulence-factors
#4
Sofie Depluverez, Simon Daled, Stijn De Waele, Sören Planckaert, Jolien Schoovaerts, Dieter Deforce, Bart Devreese
Burkholderia cenocepacia is an opportunistic pathogen that is commonly isolated from patients with cystic fibrosis (CF). Several virulence factors have been identified, including extracellular enzymes that are secreted by type II and type VI secretion systems. The activity of these secretion systems is modulated by quorum sensing. Apart from the classical acylhomoserine lactone quorum sensing, B. cenocepacia also uses the diffusible signal factor system (DSF) i.e. 2-undecenoic acid derivatives that are recognized by specific receptors resulting in changes in biofilm formation, motility and virulence...
January 11, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29322454/methods-for-measuring-the-production-of-quorum-sensing-signal-molecules
#5
Manuel Alcalde-Rico, José Luis Martínez
One relevant aspect for understanding the bottlenecks that modulate the spread of resistance among bacterial pathogens consists in the effect that the acquisition of resistance may have on the microbial physiology . Whereas studies on the effect of acquiring resistance of bacterial growth are frequently performed, more detailed analyses aiming to understand in depth the cross talk between resistance and virulence, including bacterial communication are less frequent. The bacterial quorum sensing system, is an important intraspecific and interspecific communication system highly relevant for many physiological processes, including virulence and bacterial/host interactions...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29322006/attenuation-of-quorum-sensing-dependent-virulence-factors-and-biofilm-formation-by-medicinal-plants-against-antibiotic-resistant-pseudomonas-aeruginosa
#6
P Sankar Ganesh, V Ravishankar Rai
Pseudomonas aeruginosa use small signaling molecules such as acyl homoserine lactones (AHLs), which play an important role in release virulence factors and toxin for further establishment of host infection. Thus, involving with the QS system would provide alternative ways of preventing the pathogenicity. In the present study, totally six medicinal plants (Terminalia bellerica, Celastrus paniculatus, Kingiodendron pinnatum, Schleichera oleosa, Melastoma malabathricum, Garcinia gummi-gutta) were screened for anti-QS activity using biomonitor strain of Chromobacterium violaceum CV12472...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29321722/distinct-virulent-network-between-healthcare-and-community-associated-staphylococcus-aureus-based-on-proteomic-analysis
#7
Lei He, Hongwei Meng, Qian Liu, Mo Hu, Yanan Wang, Xiaoying Chen, Xiaoyun Liu, Min Li
Background: Staphylococcus aureus (S. aureus or SA) is a leading cause of healthcare-associated (HA-) and community-associated (CA) infection. HA-SA isolates usually cause nosocomial pneumonia, bloodstream infections, catheter-related urinary tract infections, etc. On the other hand, CA-SA isolates usually cause highly fatal diseases, such as SSTIs as well as post influenza necrotic hemorrhagic pneumonia. The differences of the infection types are partially due to the unique characteristics between HA-SA and CA-SA isolates...
2018: Clinical Proteomics
https://www.readbyqxmd.com/read/29321600/fis-is-a-global-regulator-critical-for-modulation-of-virulence-factor-production-and-pathogenicity-of-dickeya-zeae
#8
Mingfa Lv, Yufan Chen, Lisheng Liao, Zhibin Liang, Zurong Shi, Yingxin Tang, Sixuan Ye, Jianuan Zhou, Lianhui Zhang
Dickeya zeae is the causal agent of rice foot rot disease, which has recently become a great threat to rice planting countries and regions. The pathogen produces a family of phytotoxins named zeamines that is critical for bacterial virulence, but little is known about the signaling pathways and regulatory mechanisms that govern zeamine production. In this study, we showed that a conserved transcriptional regulator Fis is involved in the regulation of zeamine production in D. zeae strain EC1. Deletion mutants were markedly attenuated in the virulence against rice seed germination...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320462/unsaturated-fatty-acids-affect-quorum-sensing-communication-system-and-inhibit-motility-and-biofilm-formation-of-acinetobacter-baumannii
#9
Marion Nicol, Stéphane Alexandre, Jean-Baptiste Luizet, Malena Skogman, Thierry Jouenne, Suzana P Salcedo, Emmanuelle Dé
The increasing threat of Acinetobacter baumannii as a nosocomial pathogen is mainly due to the occurrence of multidrug-resistant strains that are associated with the real problem of its eradication from hospital wards. The particular ability of this pathogen to form biofilms contributes to its persistence, increases antibiotic resistance, and promotes persistent/device-related infections. We previously demonstrated that virstatin, which is a small organic compound known to decrease virulence of Vibrio cholera via an inhibition of T4-pili expression, displayed very promising activity to prevent A...
January 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29316223/probiotic-lactobacillus-sp-inhibit-growth-biofilm-formation-and-gene-expression-of-caries-inducing-streptococcus-mutans
#10
Reham Wasfi, Ola A Abd El-Rahman, Mai M Zafer, Hossam M Ashour
Streptococcus mutans contributes significantly to dental caries, which arises from homoeostasic imbalance between host and microbiota. We hypothesized that Lactobacillus sp. inhibits growth, biofilm formation and gene expression of Streptococcus mutans. Antibacterial (agar diffusion method) and antibiofilm (crystal violet assay) characteristics of probiotic Lacobacillus sp. against Streptococcus mutans (ATCC 25175) were evaluated. We investigated whether Lacobacillus casei (ATCC 393), Lactobacillus reuteri (ATCC 23272), Lactobacillus plantarum (ATCC 14917) or Lactobacillus salivarius (ATCC 11741) inhibit expression of Streptococcus mutans genes involved in biofilm formation, quorum sensing or stress survival using quantitative real-time polymerase chain reaction (qPCR)...
January 8, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29314727/molecular-processes-underlying-synergistic-linuron-mineralization-in-a-triple-species-bacterial-consortium-biofilm-revealed-by-differential-transcriptomics
#11
Pieter Albers, Bram Weytjens, René De Mot, Kathleen Marchal, Dirk Springael
The proteobacteria Variovorax sp. WDL1, Comamonas testosteroni WDL7, and Hyphomicrobium sulfonivorans WDL6 compose a triple-species consortium that synergistically degrades and grows on the phenylurea herbicide linuron. To acquire a better insight into the interactions between the consortium members and the underlying molecular mechanisms, we compared the transcriptomes of the key biodegrading strains WDL7 and WDL1 grown as biofilms in either isolation or consortium conditions by differential RNAseq analysis...
January 3, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29314504/serine-threonine-kinase-ppka-coordinates-the-interplay-between-t6ss2-activation-and-quorum-sensing-in-the-marine-pathogen-vibrio-alginolyticus
#12
Zhen Yang, Xiaohui Zhou, Yue Ma, Mian Zhou, Matthew K Waldor, Yuanxing Zhang, Qiyao Wang
Type VI secretion systems (T6SS) are multiprotein secretion machines that can mediate killing of bacterial cells and thereby modify the composition of bacterial communities. The mechanisms that control the production of and secretion of these killing machines are incompletely understood, although quorum sensing (QS) and the PpkA kinase modulate T6SS activity in some organisms. Here we investigated control the T6S in the marine organism Vibrio alginolyticus EPGS, which encodes two T6SS systems (T6SS1 and T6SS2)...
January 4, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29308273/bacterial-bioluminescence-onset-and-quenching-a-dynamical-model-for-a-quorum-sensing-mediated-property
#13
Domenico Delle Side, Vincenzo Nassisi, Cecilia Pennetta, Pietro Alifano, Marco Di Salvo, Adelfia Talà, Aleksei Chechkin, Flavio Seno, Antonio Trovato
We present an effective dynamical model for the onset of bacterial bioluminescence, one of the most studied quorum sensing-mediated traits. Our model is built upon simple equations that describe the growth of the bacterial colony, the production and accumulation of autoinducer signal molecules, their sensing within bacterial cells, and the ensuing quorum activation mechanism that triggers bioluminescent emission. The model is directly tested to quantitatively reproduce the experimental distributions of photon emission times, previously measured for bacterial colonies of Vibrio jasicida, a luminescent bacterium belonging to the Harveyi clade, growing in a highly drying environment...
December 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29305177/cloning-expression-and-characterization-of-two-s-ribosylhomocysteine-lyases-from-lactobacillus-plantarum-ym-4-3-implication-of-conserved-and-divergent-roles-in-quorum-sensing
#14
Xiao-Dong Song, Chen-Jian Liu, Shi-Hao Huang, Xiao-Ran Li, En Yang, Yi-Yong Luo
Quorum sensing (QS) is a means of cell-to-cell communication that regulates, via small signalling molecules, expression of a series of genes and controls multicellular behaviour in many bacterial species. The enzyme S-ribosylhomocysteine lyase (LuxS) transforms S-ribosylhomocysteine (SRH) into 4, 5-dihydroxy-2, 3-pentanedione (DPD), the precursor of the interspecies QS signalling molecule autoinducer-2 (AI-2). In this study, two LuxS-coding genes, luxS1 and luxS2, with 70% sequence identity were isolated from Lactobacillus plantarum YM-4-3, and overexpressed in Escherichia coli BL21 (DE3), and the protein products were purified successfully...
January 2, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29303546/establishment-of-lcms-based-platform-for-discovery-of-quorum-sensing-inhibitors-signal-detection-in-pseudomonas-aeruginosa-pao1
#15
Manoj Kushwaha, Shreyans K Jain, Nisha Sharma, Vidushi Abrol, Sundeep Jaglan, Ram A Vishwakarma
Targeting the main three networking systems viz. Las, RhI and PQS via natural quenchers is a new ray of hope for combating the persistent behavior of Pseudomonas aeruginosa. In the bacterial chemical vocabulary pyocyanin, N-AHLs and rhamnolipids are the main keywords, which are responsible for the social and nomadic behavior of P. aeruginosa. In the present work LCMS-based real-time qualitative and quantitative analysis of pyocyanin, green phenazine, N-AHLs, and rhamnolipids were performed. The quantitative analysis indicates that the production of pyocyanin and NHSLs increases with time while the production of rhamnolipids discontinued after 16 Hrs...
January 5, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29301893/draft-genome-sequence-of-a-red-pigmented-janthinobacterium-sp-native-to-the-hudson-valley-watershed
#16
Kelsey O'Brien, Gabriel G Perron, Brooke A Jude
Water samples from the Hudson Valley watershed indicate that the area is host to many violacein-producing bacterial isolates. Here, we report the draft whole-genome sequence of Janthinobacterium sp. strain BJB412, an isolate lacking violacein production yet containing genes responsible for prodigiosin, biofilm production, and quorum sensing, like its purple-pigmented counterparts.
January 4, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29295912/role-of-heat-shock-proteases-in-quorum-sensing-mediated-regulation-of-biofilm-formation-by-vibrio-species
#17
Kyung-Jo Lee, You-Chul Jung, Soon-Jung Park, Kyu-Ho Lee
Capsular polysaccharide (CPS) is essential for the dispersal of biofilms formed by the pathogenic bacterium Vibrio vulnificus CPS production is induced by the quorum-sensing (QS) master regulator SmcR when biofilms mature. However, V. vulnificus biofilms formed under heat shock conditions did not exhibit the dispersion stage. Transcripts of the CPS gene cluster were at basal levels in the heat-exposed cell owing to reduced cellular levels of SmcR. At least two proteases induced by heat shock, ClpPA and Lon, were responsible for determining the instability of SmcR...
January 2, 2018: MBio
https://www.readbyqxmd.com/read/29290510/effect-of-nano-silver-nano-copper-deconex-and-benzalkonium-chloride-on-biofilm-formation-and-expression-of-transcription-regulatory-quorum-sensing-gene-rh1r-in-drug-resistance-pseudomonas-aeruginosa-burn-isolates
#18
Melika Gholamrezazadeh, Mohammad Reza Shakibaie, Fatemeh Monirzadeh, Shalaleh Masoumi, Zahra Hashemizadeh
BACKGROUND: Biofilm forming drug-resistant Pseudomonas aeruginosa are responsible for major death in burn center of different hospitals across the globe. OBJECTIVE: The aims of this study were to evaluate the effect of nano-silver (Ag), nano-copper (Cu), and two hospital disinfectants (deconex and benzalkonium chloride) on biofilm formation and expression of transcription regulatory quorum sensing gene rh1R in P. aeruginosa burn isolates. METHODS: 28 multidrug-resistant P...
December 28, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/29288454/detection-of-bacterial-quorum-sensing-molecules
#19
Elke Stein, Adam Schikora
Bacterial cells use the quorum sensing system to communicate with each other. The gram-negative species very often use N-acyl homoserine lactones for this purpose. One of the easiest ways to detect these molecules is the use of particular reporter strains, which possess different kinds of reporter genes under the control of AHL-responsive promoters. Here we present some of the possibilities available today, even for not specialized researchers.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29288300/biogenic-synthesis-of-silver-nanoparticles-using-piper-betle-aqueous-extract-and-evaluation-of-its-anti-quorum-sensing-and-antibiofilm-potential-against-uropathogens-with-cytotoxic-effects-an-in-vitro-and-in-vivo-approach
#20
Ramanathan Srinivasan, Loganathan Vigneshwari, Tamilselvam Rajavel, Ravindran Durgadevi, Arunachalam Kannappan, Krishnaswamy Balamurugan, Kasi Pandima Devi, Arumugam Veera Ravi
Urinary tract infections are the utmost common bacterial infections caused by Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli, and Serratia marcescens. These uropathogens resist the action of several antibiotics due to their ability to form biofilms. Most of these bacterial pathogens use the quorum sensing (QS) machinery to co-ordinate their cells and regulate several virulence factors and biofilm formation. On the other hand, the anti-quorum sensing (anti-QS) and antibiofilm potential of silver nanoparticles have been well reported against certain bacterial pathogens, but to the best of our knowledge, no report is available against the pathogenicity of uropathogens in particular S...
December 29, 2017: Environmental Science and Pollution Research International
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