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Quorum sensing

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https://www.readbyqxmd.com/read/29150517/multi-omics-reveal-the-lifestyle-of-the-acidophilic-mineral-oxidizing-model-species-leptospirillum-ferriphilum-t
#1
Stephan Christel, Malte Herold, Sören Bellenberg, Mohamed El Hajjami, Antoine Buetti-Dinh, Igor V Pivkin, Wolfgang Sand, Paul Wilmes, Ansgar Poetsch, Mark Dopson
Leptospirillum ferriphilum plays a major role in acidic, metal rich environments where it represents one of the most prevalent iron oxidizers. These milieus include acid rock and mine drainage as well as biomining operations. Despite its perceived importance, no complete genome sequence of this model species' type strain is available, limiting the possibilities to investigate the strategies and adaptations Leptospirillum ferriphilum(T) applies to survive and compete in its niche. This study presents a complete, circular genome of Leptospirillum ferriphilum(T) DSM 14647 obtained by PacBio SMRT long read sequencing for use as a high quality reference...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150484/correction-for-bortolotti-et-al-pseudomonas-aeruginosa-quorum-sensing-molecule-n-3-oxododecanoyl-l-homoserine-lactone-induces-hla-g-expression-in-human-immune-cells
#2
Daria Bortolotti, Joel LeMaoult, Claudio Trapella, Dario Di Luca, Edgardo D Carosella, Roberta Rizzo
No abstract text is available yet for this article.
December 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29144601/anti-bacterial-anti-biofilm-and-anti-quorum-sensing-effects-of-thymus-daenensis-and-satureja-hortensis-essential-oils-against-staphylococcus-aureus-isolates
#3
Aram Sharifi, Abdolmajid Mohammadzadeh, Taghi Zahraei Salehi, Pezhman Mahmoodi
AIMS: The present study was conducted to investigate the effects of Thymus daenensis and Satureja hortensis essential oils (EOs) on the planktonic growth, biofilm formation and quorum sensing (QS) of some Staphylococcus aureus isolates (strong biofilm producers). METHODS AND RESULTS: MIC of the EOs, inhibition of biofilm formation as well as disruption of preformed S. aureus biofilms were assessed. The anti-biofilm activity of the EOs was determined using microtiter-plate test (MtP) and scanning electron microscope (SEM)...
November 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29144467/progress-in-and-promise-of-bacterial-quorum-sensing-research
#4
Marvin Whiteley, Stephen P Diggle, E Peter Greenberg
This Review highlights how we can build upon the relatively new and rapidly developing field of research into bacterial quorum sensing (QS). We now have a depth of knowledge about how bacteria use QS signals to communicate with each other and to coordinate their activities. In recent years there have been extraordinary advances in our understanding of the genetics, genomics, biochemistry, and signal diversity of QS. We are beginning to understand the connections between QS and bacterial sociality. This foundation places us at the beginning of a new era in which researchers will be able to work towards new medicines to treat devastating infectious diseases, and use bacteria to understand the biology of sociality...
November 15, 2017: Nature
https://www.readbyqxmd.com/read/29143357/influence-of-phosphatidylserine-and-phosphatidylethanolamine-on-farnesol-tolerance-in-candida-albicans
#5
Sahar Hasim, Elyse N Vaughn, Dallas Donohoe, Donna M Gordon, Susan Pfiffner, Todd B Reynolds
Candida albicans is the most common human fungal pathogen. The ability to undergo the morphological transition from yeast to hyphal growth is critical for its pathogenesis. Farnesol, a precursor in the isoprenoid/sterol pathway, is a quorum-sensing molecule produced by C. albicans that inhibits hyphal growth in this polymorphic fungus. Interestingly, C. albicans can tolerate farnesol concentrations that are toxic to other fungi. We hypothesized that changes in phospholipid composition are one of the factors contributing to farnesol tolerance in C...
November 16, 2017: Yeast
https://www.readbyqxmd.com/read/29140595/modulation-of-quorum-sensing-in-a-gram-positive-pathogen-by-linear-imprinted-copolymers-with-anti-infective-properties
#6
Anfal Motib, Antonio Guerreiro, Firas Al-Bayati, Elena Piletska, Irfan Manzoor, Sulman Shafeeq, Anagha Kadam, Oscar Kuipers, Luisa Hiller, Todd Cowen, Sergey Piletsky, Peter Andrew, Hasan Yesilkaya
Here we describe the development, characterization and biological testing of a new type of linear molecularly imprinted polymer (LMIP) designed to act as anti-infective by blocking the quorum sensing (QS) mechanism and so preventing virulence of the pathogen Streptococcus pneumoniae. The LMIP is prepared (polymerized) in presence of a template molecule, but unlike in traditional molecular imprinting approaches no cross-linker is used. This results in soluble low molecular weight polymer that can act as drug agent in vitro and in vivo...
November 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29140285/chitosan-cyclodextrin-tpp-nanoparticles-loaded-with-quercetin-as-novel-bacterial-quorum-sensing-inhibitors
#7
Hao Thanh Nguyen, Francisco M Goycoolea
The widespread emergence of antibiotic-resistant bacteria has highlighted the urgent need of alternative therapeutic approaches for human and animal health. Targeting virulence factors that are controlled by bacterial quorum sensing (QS), seems a promising approach. The aims of this study were to generate novel nanoparticles (NPs) composed of chitosan (CS), sulfo-butyl-ether-β-cyclodextrin (Captisol(®)) and/or pentasodium tripolyphosphate using ionotropic gelation technique, and to evaluate their potential capacity to arrest QS in bacteria...
November 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29135456/probiotics-and-their-use-in-inflammatory-bowel-disease
#8
Muhammad Amer, Muhammad Nadeem, Saeed Ur Rashid Nazir, Maira Fakhar, Fatima Abid, Qurat-Ul Ain, Elliyah Asif
Context • Crohn's disease and ulcerative colitis result in similar gastrointestinal (GI) symptoms, including pain, diarrhea, stools with mucus or blood, and ulceration or tissue damage within the alimentary canal. Gut microbiota play a crucial role in triggering, maintaining, and exacerbating inflammatory bowel disease (IBD). Probiotics might help to rebalance the gut flora in a positive way, shifting from pro- to anti-inflammatory. Objectives • The study intended to investigate the safety and use of probiotics and the biological effects of probiotic bacteria on IBD...
November 14, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/29135250/structure-activity-relationships-of-6-and-8-gingerol-analogs-as-anti-biofilm-agents
#9
Hyunsuk Choi, So-Young Ham, Eunji Cha, Yujin Shin, Han-Shin Kim, Jeong Kyu Bang, Sang-Hyun Son, Hee-Deung Park, Youngjoo Byun
Pseudomonas aeruginosa is a causative agent of chronic infections in immunocompromised patients. Disruption of quorum sensing circuits is an attractive strategy for treating diseases associated with P. aeruginosa infection. In this study, we designed and synthesized a series of gingerol analogs targeting LasR, a master regulator of quorum sensing networks in P. aeruginosa. Structure-activity relationship studies showed that a hydrogen-bonding interaction in the head section, stereochemistry and rotational rigidity in the middle section, and optimal alkyl chain length in the tail section are important factors for the enhancement of LasR-binding affinity and for the inhibition of biofilm formation...
November 14, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29132819/quorum-sensing-systems-as-targets-for-antivirulence-therapy
#10
REVIEW
Tom Defoirdt
The development of novel therapies to control diseases caused by antibiotic-resistant pathogens is one of the major challenges we are currently facing. Many important plant, animal, and human pathogens regulate virulence by quorum sensing, bacterial cell-to-cell communication with small signal molecules. Consequently, a significant research effort is being undertaken to identify and use quorum-sensing-interfering agents in order to control diseases caused by these pathogens. In this review, an overview of our current knowledge of quorum-sensing systems of Gram-negative model pathogens is presented as well as the link with virulence of these pathogens, and recent advances and challenges in the development of quorum-sensing-interfering therapies are discussed...
November 10, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/29130186/identification-of-staphylococcal-quorum-sensing-inhibitors-by-quantification-of-%C3%A3%C2%B5-hemolysin-with-high-performance-liquid-chromatography
#11
Cassandra L Quave, Alexander R Horswill
Quorum sensing plays a major role in regulation of virulence factor production by staphylococci. Chemical inhibitors that block this process and prevent the production of exotoxins and exoenzymes could have medical utility for infection prophylaxis and therapy. Here, we describe a high performance liquid chromatography method amenable to medium throughput screening for staphylococcal quorum sensing inhibitors by quantification of õ-hemolysin, a direct protein output of this system.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130185/identification-of-ai-2-quorum-sensing-inhibitors-in-vibrio-harveyi-through-structure-based-virtual-screening
#12
Tianyu Jiang, Peng Zhu, Lupei Du, Minyong Li
Quorum sensing (QS) is a cell-to-cell communication system that regulates gene expression as a result of the production and perception of signal molecules called autoinducers (AIs). AI-2 is a QS autoinducer produced by both Gram-negative and Gram-positive bacteria, in which it regulates intraspecies and interspecies communication. The identification of QS inhibitors is considered a promising strategy for the development of anti-virulence drugs with reduced selective pressure for resistance. Here we describe a high-throughput virtual screening approach to identify AI-2 quorum sensing inhibitors on the basis of Vibrio harveyi LuxPQ crystal structure...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130184/generation-of-high-sensitivity-monoclonal-antibodies-specific-for-homoserine-lactones
#13
Soumya Palliyil
A number of bacteria use a class of chemical compounds called acyl-homoserine lactones (AHLs) as quorum sensing (QS) signals to coordinate their behavior at the population level, including pathogens like Pseudomonas aeruginosa. Blocking QS using antibodies is an attractive strategy for infection control as this process takes a central role in P. aeruginosa infections. Here the methods involved in the generation of high sensitivity anti-QS monoclonal antibodies from an immunized sheep phage display antibody library are described...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130181/a-coculture-based-approach-for-screening-campaigns-aimed-at-identifying-novel-pseudomonas-aeruginosa-quorum-sensing-inhibitors
#14
Giordano Rampioni, Giulia Giallonardi, Francesca D'Angelo, Livia Leoni
Quorum sensing (QS) is recognized as a promising target for the identification of anti-virulence drugs hampering Pseudomonas aeruginosa adaptability to the host environment and pathogenicity. Consequently, a number of studies in the last decade focused on the identification of small molecules or proteins with anti-QS activity, mainly targeting the las QS system, which is based on N-3-oxododecanoyl-homoserine lactone (3OC12-HSL) as signal molecule. Different experimental approaches have been successfully used to identify QS blockers interfering with the activity/stability of the 3OC12-HSL receptor LasR, with the functionality of the 3OC12-HSL synthase LasI, or with the stability/bioavailability of the 3OC12-HSL signal molecule itself...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130180/qualitative-and-quantitative-determination-of-quorum-sensing-inhibition-in-vitro
#15
Tim Holm Jakobsen, Maria Alhede, Louise Dahl Hultqvist, Thomas Bjarnsholt, Michael Givskov
The formation of biofilms in conjunction with quorum sensing (QS) regulated expression of virulence by opportunistic pathogens contributes significantly to immune evasion and tolerance to a variety of antimicrobial treatments. The present protocol describes methods to determine the in vitro efficacy of potential QS inhibitors (QSIs). Work on Pseudomonas aeruginosa has shown that chemical blockage of QS is a promising new antimicrobial strategy. Several live bacterial reporter systems have been developed to screen extracts and pure compounds for QSI activity...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130179/differential-equations-models-to-study-quorum-sensing
#16
Judith Pérez-Velázquez, Burkhard A Hense
Mathematical models to study quorum sensing (QS) have become an important tool to explore all aspects of this type of bacterial communication. A wide spectrum of mathematical tools and methods such as dynamical systems, stochastics, and spatial models can be employed. In this chapter, we focus on giving an overview of models consisting of differential equations (DE), which can be used to describe changing quantities, for example, the dynamics of one or more signaling molecule in time and space, often in conjunction with bacterial growth dynamics...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130178/methods-to-study-quorum-sensing-dependent-virulence-and-movement-of-phytopathogens-in-planta
#17
Shulamit Manulis-Sasson, Laura Chalupowicz
Cell-to-cell communication mediated by the diffusible signal factor (DSF) is a common form of gene regulation and plays an important role in virulence of many plant pathogenic bacteria including Xanthomonas spp. Here we describe several approaches to study the involvement of DSF-dependent QS system of the plant pathogenic bacteria Xanthomonas campestris pv. pelargonii (Xhp) as an example of the Xanthomonas spp. The methods described include detection and measurement of DSF, movement in planta, colonization, and aggregate formation...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130177/animal-models-for-pseudomonas-aeruginosa-quorum-sensing-studies
#18
Damien Maura, Arunava Bandyopadhaya, Laurence G Rahme
Quorum sensing (QS) systems play global regulatory roles in bacterial virulence. They synchronize the expression of multiple virulence factors and they control and modulate bacterial antibiotic tolerance systems and host defense mechanisms. Therefore, it is important to obtain knowledge about QS modes of action and to test putative therapeutics that may interrupt QS actions in the context of infections. This chapter describes methods to study bacterial pathogenesis in murine acute and persistent/relapsing infection models, using the Gram-negative bacterial pathogen Pseudomonas aeruginosa as an example...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130176/assessing-pseudomonas-aeruginosa-autoinducer-effects-on-mammalian-epithelial-cells
#19
Jake Everett, Rebecca Gabrilska, Kendra P Rumbaugh, Elena Vikström
The human mucosal environment in the gut is rich with interactions between microbiota and mammalian epithelia. Microbes such as the Gram-negative bacterium Pseudomonas aeruginosa may use quorum sensing to communicate with other microorganisms and mammalian cells to alter gene expression. Here, we present methodologies to evaluate the effects of P. aeruginosa N-(3-oxo-dodecanoyl)-L-homoserine lactone (3O-C12-HSL) on Caco-2 cell monolayers. First, we describe a method for assessing barrier function and permeability of epithelial cells when exposed to 3O-C12-HSL by measuring transepithelial electrical resistance (TER) and paracellular flow using fluorescently labeled dextran...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130175/imaging-n-acyl-homoserine-lactone-quorum-sensing-in-vivo
#20
Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt
In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E...
2018: Methods in Molecular Biology
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