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https://www.readbyqxmd.com/read/29241296/evolving-aptamers-with-unnatural-base-pairs
#1
Michiko Kimoto, Ken-Ichiro Matsunaga, Ichiro Hirao
A novel technology, genetic alphabet expansion, has rapidly advanced through the successful creation of unnatural base pairs that function as a third base pair in replication. Recently, genetic alphabet expansion has been applied to some practical areas. Among them, the application to DNA aptamer generation is a good example of the broad utility of this technology. A hydrophobic unnatural base pair, Ds-Px, which exhibits high fidelity in replication as a third base pair, has been applied to an evolutionary engineering method called SELEX (Systematic Evolution of Ligands by EXponential enrichment) to generate DNA aptamers that bind to targets...
December 14, 2017: Current Protocols in Chemical Biology
https://www.readbyqxmd.com/read/29241087/development-of-a-fast-hplc-dad-method-for-simultaneous-quantitation-of-three-immunosuppressant-drugs-in-whole-blood-samples-using-intelligent-chemometrics-resolving-of-coeluting-peaks-in-the-presence-of-blood-interferences
#2
Maryam Vosough, Sadaf Mosleh Tehrani
The present study describes a fast high performance liquid chromatography-diode array detection analytical methodology for quantification of tacrolimus, everolimus and cyclosporine A in whole blood samples, with minimum sample preparation steps. A short isocratic chromatographic elution was coupled with second-order calibration using multivariate curve resolution to stablish a smart and green methodology. Due to presence of matrix effect, a sample-added calibration strategy was used for quantification purposes...
December 9, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29240819/use-of-thermal-analysis-coupled-with-differential-scanning-calorimetry-quadrupole-mass-spectrometry-and-infrared-spectroscopy-tg-dsc-qms-ftir-to-monitor-chemical-properties-and-thermal-stability-of-fulvic-and-humic-acids
#3
Patrycja Boguta, Zofia Sokołowska, Kamil Skic
Thermogravimetry-coupled with differential scanning calorimetry, quadrupole mass spectrometry, and Fourier-transform infrared spectroscopy (TG-DSC-QMS-FTIR)-was applied to monitor the thermal stability (in an N2 pyrolytic atmosphere) and chemical properties of natural polymers, fulvic (FA) and humic acids (HA), isolated from chemically different soils. Three temperature ranges, R1, 40-220°C; R2, 220-430°C; and R3, 430-650°C, were distinguished from the DSC data, related to the main thermal processes of different structures (including transformations without weight loss)...
2017: PloS One
https://www.readbyqxmd.com/read/29240665/airborne-or-fomite-transmission-for-norovirus-a-case-study-revisited
#4
Shenglan Xiao, Julian W Tang, Yuguo Li
Norovirus infection, a highly prevalent condition associated with a high rate of morbidity, comprises a significant health issue. Although norovirus transmission mainly occurs via the fecal-oral and vomit-oral routes, airborne transmission has been proposed in recent decades. This paper re-examines a previously described norovirus outbreak in a hotel restaurant wherein airborne transmission was originally inferred. Specifically, the original evidence that suggested airborne transmission was re-analyzed by exploring an alternative hypothesis: could this outbreak instead have occurred via fomite transmission? This re-analysis was based on whether fomite transmission could have yielded similar attack rate distribution patterns...
December 14, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29240542/phase-ii-study-of-everolimus-in-patients-with-thymoma-and-thymic-carcinoma-previously-treated-with-cisplatin-based-chemotherapy
#5
Paolo Andrea Zucali, Tommaso De Pas, Giovannella Palmieri, Adolfo Favaretto, Antonio Chella, Marcello Tiseo, Michele Caruso, Matteo Simonelli, Matteo Perrino, Fabio De Vincenzo, Francesca Toffalorio, Vincenzo Damiano, Giulia Pasello, Erika Garbella, Marco Ali, Fabio Conforti, Margaret Ottaviano, Angela Cioffi, Sabino De Placido, Laura Giordano, Monica Bertossi, Annarita Destro, Luca Di Tommaso, Armando Santoro
Purpose No effective salvage treatments are available for patients with advanced/recurrent thymoma (T) or thymic carcinoma (TC) who have progressed after platinum-based chemotherapy. This study evaluated the activity of everolimus in patients with advanced/recurrent T or TC previously treated with cisplatin-containing chemotherapy. Patients and Methods This was a single-arm, single-stage, open-label, multicenter, phase II trial. Patients received oral everolimus 10 mg/d until disease progression, unacceptable toxicity, or patient refusal...
December 14, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/29240397/functionalization-of-sio2-surfaces-for-si-monolayer-doping-with-minimal-carbon-contamination
#6
Maart van Dreunen, Gillian Collins, Colm Glynn, Colm O'Dwyer, Justin D Holmes
Monolayer Doping (MLD) involves the functionalization of semiconductor surfaces followed by an annealing step to diffuse the dopant into the substrate. We report an alternative doping method, oxide-MLD, where ultra-thin SiO2 overlayers are functionalized with phosphonic acids for doping Si. Similar peak carrier concentrations were achieved when compared to hydrosilylated surfaces (~2 × 1020 atoms/cm3). Oxide-MLD offers several advantages over conventional MLD such as ease of sample processing, superior ambient stability and minimal carbon contamination...
December 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29240071/-remote-results-of-open-surgical-and-endovascular-treatment-of-internal-carotid-artery-stenoses
#7
REVIEW
R A Vinogradov, V S Pykhteev, K A Lashevich
The article is a review containing a comparative analysis of the remote results in patients presenting with carotid artery atherosclerosis and treated by means of either stenting or carotid endarterectomy. More than ten international randomized studies have up to now been conducted. Some of them prove the advantage of using either carotid endarterectomy or stenting of carotid arteries, others show equivalence of both methods of treatment. Carotid endarterectomy is currently a preferred operation for carotid artery stenoses, with lower incidence of the development of postoperative strokes and restenoses in the remote period...
2017: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/29240057/-experience-with-using-cyanoacrylate-glue-in-endovascular-treatment-of-varicose-veins
#8
E V Shaĭdakov, A Zh Mel'tsova, O Ia Porembskaia, E A Kudinova, D É Korzhevskiĭ, O V Kirik, E G Sukhorukova
The method of cyanoacrylate-mediated obliteration of subcutaneous veins is known to be an alternative to thermal endovascular obliteration and eliminates the need for tumescent anaesthesia. This technique is based on glue-induced damage to the venous intima, followed by immune response according to the delayed-type hypersensitivity principle. The authors report herein their first experience with using cyanoacrylate-mediated embolization in treatment of patients presenting with varicose veins. The operation was carried out using the VenaSeal closure system (Medtronic)...
2017: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/29239987/body-composition-of-women-s-ice-hockey-players-comparison-of-estimates-using-skinfolds-and-idxa
#9
Kajsa Gilenstam, Christina A Geithner
The purpose of this study was to compare percent fat (% Fat) estimates from anthropometric equations using skinfolds (SKF) in women's ice hockey players to estimates obtained from Lunar iDXA. Data were collected on 19 elite female Swedish hockey players (mean age ± SD = 18.4 ± 2.4 y). Four skinfolds (SKF) (triceps, abdominal, suprailiac, and thigh) were measured within two hours of iDXA assessments. The % Fat estimates from iDXA and four anthropometric equations were compared using paired t-tests, and a one-way ANOVA was used to compare % Fat estimates from the anthropometric equations...
December 11, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29239982/postexercise-hypotension-and-heart-rate-variability-responses-subsequent-to-traditional-paired-set-and-superset-training-methods
#10
Gabriel A Paz, Eliseo Iglesias-Soler, Jeffrey M Willardson, Marianna de Freitas Maia, Humberto Miranda
The purpose of this study was to compare training volume, post-exercise hypotension (PEH) and heart rate variability (HRV) responses to different strength training methods. Thirteen trained men volunteered for this study. Three training methods were completed in a randomized design, which included: Traditional Set (TS) - three successive sets for the lying bench press (LBP), lat pulldown (LPD), incline 45° bench press (BP45), seated close-grip row (SCR), triceps extension (TE), and biceps curl (BC), with a 90 sec...
December 11, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29239965/videolaryngoscopy-for-physician-based-prehospital-emergency-intubation-a-prospective-randomized-multicenter-comparison-of-different-blade-types-using-a-p-advance-c-mac-system-and-kingvision
#11
Erol Cavus, Sebastian Janssen, Florian Reifferscheid, Amke Caliebe, Andreas Callies, Martin von der Heyden, Peer G Knacke, Volker Doerges
BACKGROUND: Videolaryngoscopy is a valuable technique for endotracheal intubation. When used in the perioperative period, different videolaryngoscopes vary both in terms of technical use and intubation success rates. However, in the prehospital environment, the relative performance of different videolaryngoscopic systems is less well studied. METHODS: We conducted this prospective, randomized, multicenter study at 4 German prehospital emergency medicine centers...
December 11, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/29239871/integrative-medicine-as-an-adjunct-to-orthopaedic-surgery
#12
James R Ficke, Nathan M Moroski, Steven D Ross, Ranjan Gupta
Patients often seek nontraditional forms of treatment, including alternative/complementary medical options, such as chiropractic care and acupuncture, to meet their personal needs. In the United States, interest has grown in methods to reduce pain and improve function through Ayurvedic medicine, which uses plant-based supplements, such as turmeric. Traditional allopathic medicine attempts to provide patients with evidence-based therapeutic regimens for their musculoskeletal conditions. Integrative medicine often is used to prevent and manage the sequelae associated with injuries and illnesses; however, competitive athletes and military personnel use complementary medicine for performance enhancement...
December 11, 2017: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/29239600/exonuclease-iii-assisted-upconversion-resonance-energy-transfer-in-a-wash-free-suspension-dna-assay
#13
Yinghui Chen, Hien Duong, Shihui Wen, Chao Mi, Yingzhu Zhou, Olga Shimoni, Stella M Valenzuela, Dayong Jin
Sensitivity is the key in optical detection of low-abundant analytes, such as circulating RNA or DNA. The enzyme Exonuclease III (Exo III) is a useful tool in this regard; its ability to recycle target DNA molecules results in markedly improved detection sensitivity. Lower limits of detection may be further achieved if the detection background of auto-fluorescence can be removed. Here we report an ultrasensitive and specific method to quantify trace amounts of DNA analytes in a wash-free suspension assay. In the presence of target DNA, the Exo III recycles the target DNA by selectively digesting the dye-tagged sequence-matched probe DNA strand only, so that the amount of free dye removed from the probe DNA is proportional to the number of target DNAs...
December 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29239554/cutl-an-alternative-to-kulldorff-s-scan-statistics-for-cluster-detection-with-a-specified-cut-off-level
#14
Barbara Więckowska, Justyna Marcinkowska
When searching for epidemiological clusters, an important tool can be to carry out one's own research with the incidence rate from the literature as the reference level. Values exceeding this level may indicate the presence of a cluster in that location. This paper presents a method of searching for clusters that have significantly higher incidence rates than those specified by the investigator. The proposed method uses the classic binomial exact test for one proportion and an algorithm that joins areas with potential clusters while reducing the number of multiple comparisons needed...
November 6, 2017: Geospatial Health
https://www.readbyqxmd.com/read/29239496/two-phase-designs-for-joint-quantitative-trait-dependent-and-genotype-dependent-sampling-in-post-gwas-regional-sequencing
#15
Osvaldo Espin-Garcia, Radu V Craiu, Shelley B Bull
We evaluate two-phase designs to follow-up findings from genome-wide association study (GWAS) when the cost of regional sequencing in the entire cohort is prohibitive. We develop novel expectation-maximization-based inference under a semiparametric maximum likelihood formulation tailored for post-GWAS inference. A GWAS-SNP (where SNP is single nucleotide polymorphism) serves as a surrogate covariate in inferring association between a sequence variant and a normally distributed quantitative trait (QT). We assess test validity and quantify efficiency and power of joint QT-SNP-dependent sampling and analysis under alternative sample allocations by simulations...
December 14, 2017: Genetic Epidemiology
https://www.readbyqxmd.com/read/29239068/estimating-chronic-hepatitis-c-prognosis-using-transient-elastography-based-liver-stiffness-a-systematic-review-and-meta-analysis
#16
Aysegul Erman, Abhinay Sathya, Austin Nam, Joanna M Bielecki, Jordan J Feld, Hla-Hla Thein, William W L Wong, Paul Grootendorst, Murray D Krahn
Chronic hepatitis C (CHC) is a leading cause of hepatic fibrosis and cirrhosis. The level of fibrosis is traditionally established by histology and prognosis is estimated using fibrosis progression rates (FPRs; annual probability of progressing across histological stages). However, newer non-invasive alternatives are quickly replacing biopsy. One alternative, transient elastography (TE), quantifies fibrosis by measuring liver stiffness (LSM). Given these developments, the purpose of this study was (1) to estimate prognosis in treatment-naïve CHC patients using TE-based liver stiffness progression rates (LSPR) as an alternative to FPRs and (2) to compare consistency between LSPRs and FPRs...
December 14, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29238853/detection-and-genotyping-of-hpv-in-urine-samples-from-chilean-women-attending-primary-health-care-centers
#17
Nicolás Vergara, Monserrat Balanda, Wilma Hidalgo, Héctor San Martín, Alexis Aceituno, Francisco Roldán, Tania Villalón, Melissa Hott, Gloria Espinoza, Andrea Quiero, María T Valenzuela, Eugenio Ramírez
Cervical cancer is the second most common malignant neoplasm in women worldwide representing approximately 10% of all types of cancers. Triage of women through cervical cytology has been an important strategy for the surveillance and control of new cases of cervical cancer. However, in many regions around the world cervical cytology has a low coverage compared to developed countries. The molecular detection of HPV is the most effective method to increase the screening sensitivity of women at risk of developing cervical cancer...
December 14, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29238555/residence-time-native-range-size-and-genome-size-predict-naturalization-among-angiosperms-introduced-to-australia
#18
John P Schmidt, John M Drake, Patrick Stephens
Although critical to progress in understanding (i) if, and (ii) at what rate, introduced plants will naturalize and potentially become invasive, establishing causal links between traits and invasion success is complicated by data gaps, phylogenetic nonindependence of species, the inability to control for differences between species in residence time and propagule pressure, and covariance among traits. Here, we focus on statistical relationships between genomic factors, life history traits, native range size, and naturalization status of angiosperms introduced to Australia...
December 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/29238483/the-influence-of-polishing-techniques-on-pre-polymerized-cad-cam-acrylic-resin-denture-bases
#19
Manal Rahma Alammari
Background: Lately, computer-aided design and computer-aided manufacturing (CAD/CAM) has broadly been successfully employed in dentistry. The CAD/CAM systems have recently become commercially available for fabrication of complete dentures, and are considered as an alternative technique to conventionally processed acrylic resin bases. However, they have not yet been fully investigated. Objective: The purpose of this study was to inspect the effects of mechanical polishing and chemical polishing on the surface roughness (Ra) and contact angle (wettability) of heat-cured, auto-cured and CAD/CAM denture base acrylic resins...
October 2017: Electronic Physician
https://www.readbyqxmd.com/read/29238257/validation-of-an-arm-crank-ergometer-test-for-use-in-sedentary-adults
#20
Alexandros Mitropoulos, Anil Gumber, Helen Crank, Markos Klonizakis
The maximal oxygen uptake (V̇O2peak) test is an approved pre-operative examination tool, in a clinical setting: Both V̇O2peak and anaerobic threshold indicate a patient's physiological tolerance for major surgery and post-operative mortality, with cycle ergometry being routinely used for V̇O2peak tests in clinical settings, in many European countries. Nevertheless, the opportunities to assess populations with restricted mobility of the lower limbs are limited, as alternative methods (such as an arm-crank test protocol) to assess V̇O2peak are yet to be established...
December 2017: Journal of Sports Science & Medicine
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