keyword
MENU ▼
Read by QxMD icon Read
search

alternative methods

keyword
https://www.readbyqxmd.com/read/28651382/assignment-of-sigma-factors-of-rna-polymerase-to-promoters-in-corynebacterium-glutamicum
#1
Hana Dostálová, Jiří Holátko, Tobias Busche, Lenka Rucká, Andrey Rapoport, Petr Halada, Jan Nešvera, Jörn Kalinowski, Miroslav Pátek
Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (σ(A)), the primary-like σ(B) and five alternative sigma factors (σ(C), σ(D), σ(E), σ(H) and σ(M)). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. In vitro transcription assays and measurements of promoter activity using the overexpression of a single sigma factor gene and the transcriptional fusion of the promoter to the gfpuv reporter gene enabled us to reliably define the sigma factor dependency of promoters...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28651276/white-paper-interventional-mri-current-status-and-potential-for-development-considering-economic-perspectives-part-1-general-application
#2
Jörg Barkhausen, Thomas Kahn, Gabriele A Krombach, Christiane K Kuhl, Joachim Lotz, David Maintz, Jens Ricke, Stefan O Schönberg, Thomas J Vogl, Frank K Wacker
Background MRI is attractive for the guiding and monitoring of interventional procedures due to its high intrinsic soft tissue contrast and the possibility to measure physiologic parameters like flow and cardiac function. Method The current status of interventional MRI for the clinical routine was analyzed. Results The effort needed for the development of MR-safe monitoring systems and instruments initially resulted in the application of interventional MRI only for procedures that could not be performed by other means...
July 2017: RöFo: Fortschritte Auf Dem Gebiete der Röntgenstrahlen und der Nuklearmedizin
https://www.readbyqxmd.com/read/28651199/aberration-corrected-stem-by-means-of-diffraction-gratings
#3
Martin Linck, Peter A Ercius, Jordan S Pierce, Benjamin J McMorran
In the past 15 years, the advent of aberration correction technology in electron microscopy has enabled materials analysis on the atomic scale. This is made possible by precise arrangements of multipole electrodes and magnetic solenoids to compensate the aberrations inherent to any focusing element of an electron microscope. Here, we describe an alternative method to correct for the spherical aberration of the objective lens in scanning transmission electron microscopy (STEM) using a passive, nanofabricated diffractive optical element...
June 12, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28651175/challenges-in-gc-ms-analysis-case-studies-on-phenibut-and-ethylphenidate
#4
Hui Zhi Shirley Lee, Mei Ching Ong, Jong Lee Wendy Lim, Tiong Whei Angeline Yap
The challenges associated with drug analysis using GC-MS such as thermal degradation, cyclisation or unwanted side reactions causing potential erroneous identification have become evident in view of the high surge in new drugs available in the market. Two case studies illustrated how alternative methods or modifications to existing techniques can help to circumvent the limitations. In the first case study, phenibut which is a GABA analogue, cyclises to 4-phenyl-2-pyrrolidinone under thermal conditions. The identification of phenibut was achieved through derivatisation and identification of its TMS derivative...
June 9, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28651094/in-vitro-susceptibility-of-brachyspira-hyodysenteriae-to-a-commercial-citrus-fruit-extract
#5
Pedro J G de Nova, Ana Carvajal, Miguel Prieto, Pedro Rubio
Brachyspira hyodysenteriae is the main etiological agent of swine dysentery (SD). Nowadays, treatment and control of SD is increasingly difficult due to the emergence of antimicrobial resistance together with the restrictions on the use of antibiotics in veterinary practice. The aim of this study was to evaluate, as an alternative in the control of this disease, the antimicrobial activity and the main mechanism of action of BIOCITRO, a citrus extract commercialized as raw material and used as feed additive, against B...
June 20, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28651073/hemophilia-a-inhibitor-treatment-the-promise-of-engineered-t-cell-therapy
#6
REVIEW
Kalpana Parvathaneni, Maha Abdeladhim, Kathleen P Pratt, David W Scott
Hemophilia A is a bleeding disorder caused by mutations in the gene encoding factor VIII (FVIII), a cofactor protein that is essential for normal blood clotting. Approximately, 1 in 3 patients with severe hemophilia A produce neutralizing antibodies (inhibitors) that block its biologic function in the clotting cascade. Current efforts to eliminate inhibitors consist of repeated FVIII injections under what is termed an "ITI" protocol (Immune Tolerance Induction). However, this method is extremely costly and approximately 30% of patients undergoing ITI do not achieve peripheral tolerance...
June 9, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28650991/distinguishing-functional-polymorphism-from-random-variation-in-the-sequences-of-10-000-hla-a-b-and-c-alleles
#7
James Robinson, Lisbeth A Guethlein, Nezih Cereb, Soo Young Yang, Paul J Norman, Steven G E Marsh, Peter Parham
HLA class I glycoproteins contain the functional sites that bind peptide antigens and engage lymphocyte receptors. Recently, clinical application of sequence-based HLA typing has uncovered an unprecedented number of novel HLA class I alleles. Here we define the nature and extent of the variation in 3,489 HLA-A, 4,356 HLA-B and 3,111 HLA-C alleles. This analysis required development of suites of methods, having general applicability, for comparing and analyzing large numbers of homologous sequences. At least three amino-acid substitutions are present at every position in the polymorphic α1 and α2 domains of HLA-A, -B and -C...
June 26, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28650961/mfpred-rapid-and-accurate-prediction-of-protein-peptide-recognition-multispecificity-using-self-consistent-mean-field-theory
#8
Aliza B Rubenstein, Manasi A Pethe, Sagar D Khare
Multispecificity-the ability of a single receptor protein molecule to interact with multiple substrates-is a hallmark of molecular recognition at protein-protein and protein-peptide interfaces, including enzyme-substrate complexes. The ability to perform structure-based prediction of multispecificity would aid in the identification of novel enzyme substrates, protein interaction partners, and enable design of novel enzymes targeted towards alternative substrates. The relatively slow speed of current biophysical, structure-based methods limits their use for prediction and, especially, design of multispecificity...
June 26, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28650814/multi-label-classification-by-semi-supervised-singular-value-decomposition
#9
Liping Jing, Chenyang Shen, Liu Yang, Jian Yu, Michael K Ng
Multi-label problems arise in various domains including automatic multimedia data categorization, and have generated significant interest in computer vision and machine learning community. However, existing methods do not adequately address two key challenges: exploiting correlations between labels and making up for the lack of labeled data or even missing labeled data. In this paper, we proposed to use a semi-supervised singular value decomposition to handle these two challenges. The proposed model takes advantage of the nuclear norm regularization on the singular value decomposition to effectively capture the label correlations...
June 26, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28650508/pblnb7o13-ln-tb-or-eu-a-new-type-of-layered-polyborate-with-multi-colour-light-emission-properties
#10
Dan Zhao, Fa-Xue Ma, Yun-Chang Fan, Lei Zhang, Rui-Juan Zhang, Pei-Gao Duan
Two new lead rare-earth polyborates, PbTbB7O13 and PbEuB7O13, have been successfully synthesized via a high temperature molten salt method. Single crystal X-ray diffraction analysis reveals that they are isostructural and feature a 2D layer structure that contains alternating layers of [B7O13]∞ and [Tb]∞. The [B7O13]∞ layer is constructed of BO3 and BO4 groups with the fundamental building block of B7O17 (3Δ4□: <Δ2□>Δ<Δ2□>). Solid solutions of PbTb1-xEuxB7O13 (x = 0-1) were prepared via a solid state reaction and the photoluminescence properties were studied...
June 26, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28650487/engineering-thiophene-based-nanoparticles-to-induce-phototransduction-in-live-cells-under-illumination
#11
M Zangoli, F Di Maria, E Zucchetti, C Bossio, M R Antognazza, G Lanzani, R Mazzaro, F Corticelli, M Baroncini, G Barbarella
We report that nanoparticles prepared from appropriately functionalized polythiophenes once administered to live cells can acquire phototransduction properties under illumination, becoming photoactive sites able to absorb visible light and convert it to an electrical signal through cell membrane polarization. Amine-reactive fluorescent nanoparticles with pendant N-succinimidyl-ester groups (NPs-NHS) are prepared from polythiophenes alternating unsubstituted and 3-(2,5-dioxopyrrolidin-1-yl-8-octanoate)-substituted thiophenes by a nanoprecipitation method...
June 26, 2017: Nanoscale
https://www.readbyqxmd.com/read/28650324/formulation-evaluation-and-release-rate-characteristics-of-medicated-jelly-of-vitamin-c
#12
Khalid A Ibrahim, Asma Nawaz, Shamim Mumtaz, Furqan Muhammad Iqbal, Ahmad Khan, Shahiquz Zaman, Nasser M Abd El-Salam, Naseem Ullah, Tajamul Husain, Syed Nisar Hussain Shah
Medicated jelly formulations are patient friendly dosage form for pediatric, geriatric and dysphagic patients. These formulations offer rapid dissolution and absorption of drugs through oral mucosa therefore show the early onset of action. The objective of the study was to develop and evaluate oral jelly formulations of vitamin C. Slurry method was adopted using glucose 103gm, sugar 67gm, gelatin 10gm and sorbitol 6.56gm. Preformulation studies were performed including the organoleptic profile, pH, and solubility of both drugs...
March 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28650155/probing-interface-defects-in-top-gated-mos2-transistors-with-impedance-spectroscopy
#13
Peng Zhao, Angelica Azcatl, Yuri Y Gomeniuk, Pavel Bolshakov, Michael Schmidt, Stephen J McDonnell, Christopher L Hinkle, Paul K Hurley, Robert M Wallace, Chadwin Young
The electronic properties of the HfO2/MoS2 interface were investigated using multi-frequency capacitance-voltage (C-V) and current-voltage characterization of top gated MoS2 metal oxide semiconductor field effect transistors. The analysis was performed on few layer (5 - 10) MoS2 MOSFETs fabricated using photolithographic patterning with 13 nm and 8 nm HfO2 gate oxide layers formed by atomic layer deposition after in-situ UV-O3 surface functionalization. The impedance response of the HfO2/MoS2 gate stack indicates the existence of specific defects at the interface, which exhibited either a frequency dependent distortion similar to conventional Si MOSFETs with unpassivated silicon dangling bonds, or a frequency dispersion over the entire voltage range corresponding to depletion of the HfO2/MoS2 surface, consistent with interface traps distributed over a range of energy levels...
June 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28650133/imaging-in-primary-hyperparathyroidism-focus-on-the-evidence-based-diagnostic-performance-of-different-methods
#14
Giorgio Treglia, Pierpaolo Trimboli, Martin Huellner, Luca Giovanella
BACKGROUND: Primary hyperparathyroidism (PHPT) is a common endocrine disorder usually due to hyperfunctioning parathyroid glands (HP). Surgical removal of HP is the main treatment in PHPT, particularly in symptomatic patients. The correct detection and localization of HP is challenging and crucial as it may guide surgical treatment in patients with PHPT. To date, different imaging methods have been used to detect and localize HP in patients with PHPT including radiology, nuclear medicine and hybrid techniques...
June 23, 2017: Minerva Endocrinologica
https://www.readbyqxmd.com/read/28650129/in-vitro-efficacy-and-in-silico-analysis-of-cefixime-ofloxacin-combination-for-salmonella-typhi-from-bloodstream-infection
#15
Yamuna Devi Bakthavatchalam, Thirumal Kumar D, Iftikhar Aslam Tayubi, Baby Abirami S, Priyanka Babu, Elakkiya Munusamy, Bhuvaneswari Thukkaram, Raji Ravi, George Priya Doss C, Balaji Veeraraghavan
AIMS: Recently, the cefixime-ofloxacin combination is approved by drug controller general of India (DCGI) to treat typhoid fever. We sought to evaluate the antimicrobial activity of cefixime-ofloxacin combination against S. Typhi. METHODS AND RESULTS: 283 non-duplicate S. Typhi isolates collected during 2012 to 2014 were included in this study. Minimum inhibitory concentration (MIC) of cefixime and ofloxacin was determined by using broth microdilution method. Combinational testing was performed by using checkerboard assay...
June 26, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28650108/a-matched-pair-analysis-of-multicenter-long-term-follow-up-after-split-liver-transplantation-with-extended-right-grafts
#16
M W Ross, M Cescon, R Angelico, E Andorno, G Rossi, A Pinna, L De Carlis, U Baccarani, U Cillo, M Colledan, V Mazzaferro, G Tisone, M Rossi, F Tuzzolino, D Pagano, S Gruttadauria, G Mazariegos, B Gridelli, M Spada
Split liver transplantation has been proposed as an alternative to whole liver transplantation to expand the donor pool, but studies comparing adult long-term outcomes between the two methods are conflicting and limited. This is the first Italian multicenter study that retrospectively analyzed 119 matched-pair recipients of whole and extended right grafts for long-term survival outcomes. In the overall population, whole liver recipients showed higher patient survival at one- (93% vs. 73%), five- (87% vs. 65%) and ten-year (83% vs...
June 26, 2017: Liver Transplantation
https://www.readbyqxmd.com/read/28649976/efficiency-of-analytical-and-sampling-based-uncertainty-propagation-in-intensity-modulated-proton-therapy
#17
N Wahl, P Hennig, H P Wieser, M Bangert
The sensitivity of intensity-modulated proton therapy (IMPT) treatment plans to uncertainties can be quantified and mitigated with robust/min-max and stochastic/probabilistic treatment analysis and optimization techniques. Those methods usually rely on sparse random, importance, or worst-case sampling. Inevitably, this imposes a trade-off between computational speed and accuracy of the uncertainty propagation. Here, we investigate analytical probabilistic modeling (APM) as an alternative for uncertainty propagation and minimization in IMPT that does not rely on scenario sampling...
June 26, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28649599/semi-automated-annotation-of-signal-events-in-clinical-eeg-data
#18
S Yang, S López, M Golmohammadi, I Obeid, J Picone
To be effective, state of the art machine learning technology needs large amounts of annotated data. There are numerous compelling applications in healthcare that can benefit from high performance automated decision support systems provided by deep learning technology, but they lack the comprehensive data resources required to apply sophisticated machine learning models. Further, for economic reasons, it is very difficult to justify the creation of large annotated corpora for these applications. Hence, automated annotation techniques become increasingly important...
December 2016: ... IEEE Signal Processing in Medicine and Biology Symposium. IEEE Signal Processing in Medicine and Biology Symposium
https://www.readbyqxmd.com/read/28649577/in%C3%A2-vivo-murine-matured-human-cd3-cells-as-a-preclinical-model-for-t-cell-based-immunotherapies
#19
Kevin G Haworth, Christina Ironside, Zachary K Norgaard, Willimark M Obenza, Jennifer E Adair, Hans-Peter Kiem
Adoptive cellular immunotherapy is a promising and powerful method for the treatment of a broad range of malignant and infectious diseases. Although the concept of cellular immunotherapy was originally proposed in the 1990s, it has not seen successful clinical application until recent years. Despite significant progress in creating engineered receptors against both malignant and viral epitopes, no efficient preclinical animal models exist for rapidly testing and directly comparing these engineered receptors...
September 15, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/28649431/meta-analysis-of-host-response-networks-identifies-a-common-core-in-tuberculosis
#20
Awanti Sambarey, Abhinandan Devaprasad, Priyanka Baloni, Madhulika Mishra, Abhilash Mohan, Priyanka Tyagi, Amit Singh, J S Akshata, Razia Sultana, Shashidhar Buggi, Nagasuma Chandra
Tuberculosis remains a major global health challenge worldwide, causing more than a million deaths annually. To determine newer methods for detecting and combating the disease, it is necessary to characterise global host responses to infection. Several high throughput omics studies have provided a rich resource including a list of several genes differentially regulated in tuberculosis. An integrated analysis of these studies is necessary to identify a unified response to the infection. Such data integration is met with several challenges owing to platform dependency, patient heterogeneity, and variability in the extent of infection, resulting in little overlap among different datasets...
2017: NPJ Systems Biology and Applications
keyword
keyword
30447
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"