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https://www.readbyqxmd.com/read/28539002/from-in-vivo-to-in-vitro-the-medical-device-testing-paradigm-shift
#1
Dayna Kerecman Myers, Alan M Goldberg, Albrecht Poth, Michael F Wolf, Joseph Carraway, James McKim, Kelly P Coleman, Richard Hutchinson, Ronald Brown, Harald F Krug, Anthony Bahinski, Thomas Hartung
Amid growing efforts to advance the replacement, reduction, and refinement of the use of animals in research, there is a growing recognition that in vitro testing of medical devices can be more effective, both in terms of cost and time, and also more reliable than in vivo testing. Although the technological landscape has evolved rapidly in support of these concepts, regulatory acceptance of alternative testing methods has not kept pace. Despite the acceptance by regulators of some in vitro tests (cytotoxicity, gene toxicity, and some hemocompatibility assays), many toxicity tests still rely on animals (irritation, sensitization, acute toxicity, reproductive/developmental toxicity), even where other industrial sectors have already abandoned them...
May 25, 2017: ALTEX
https://www.readbyqxmd.com/read/28538671/engineering-exosomes-for-cancer-therapy
#2
REVIEW
Katie E Gilligan, Róisín M Dwyer
There remains an urgent need for novel therapeutic strategies to treat metastatic cancer, which results in over 8 million deaths annually worldwide. Following secretion, exosomes are naturally taken up by cells, and capable of the stable transfer of drugs, therapeutic microRNAs and proteins. As knowledge of the biogenesis, release and uptake of exosomes continues to evolve, and thus also has interest in these extracellular vesicles as potential tumor-targeted vehicles for cancer therapy. The ability to engineer exosome content and migratory itinerary holds tremendous promise...
May 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28538295/relationship-between-internal-load-indicators-and-changes-on-intermittent-performance-after-the-preseason-in-professional-soccer-players
#3
Miguel A Campos-Vazquez, Francisco J Toscano-Bendala, José C Mora-Ferrera, Luis J Suarez-Arrones
Campos-Vazquez, MA, Toscano-Bendala, FJ, Mora-Ferrera, JC, and Suarez-Arrones, LJ. Relationship between internal load indicators and changes on intermittent performance after the preseason in professional soccer players. J Strength Cond Res 31(6): 1477-1485, 2017-The aim of this study was to examine the effects of accumulated internal training load (ITL) during the preseason (4 weeks) on changes in the intermittent performance, in a professional soccer team. Twelve professionals soccer players (Mean ± SD age: 27...
June 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28538294/effects-of-different-combinations-of-strength-power-and-plyometric-training-on-the-physical-performance-of-elite-young-soccer-players
#4
Ronaldo Kobal, Irineu Loturco, Renato Barroso, Saulo Gil, Rogério Cuniyochi, Carlos Ugrinowitsch, Hamilton Roschel, Valmor Tricoli
Kobal, R, Loturco, I, Barroso, R, Gil, S, Cuniyochi, R, Ugrinowitsch, C, Roschel, H, and Tricoli, V. Effects of different combinations of strength, power, and plyometric training on the physical performance of elite young soccer players. J Strength Cond Res 31(6): 1468-1476, 2017-The combination of strength (ST) and plyometric training (PT) has been shown to be effective for improving sport-specific performance. However, there is no consensus about the most effective way to combine these methods in the same training session to produce greater improvements in neuromuscular performance of soccer players...
June 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28538191/assessment-of-hyperspectral-imaging-system-for-colour-measurement
#5
Damian K Chlebda, Anna Rogulska, Tomasz Łojewski
A novel technique of reflective spectroscopy, hyperspectral imaging (HSI), was used to develop a methodology for colour measurement, which is very important in the field of art conservation - especially in the analysis of documents. The major focus of this work is an examination of the repeatability and reproducibility of colour parameters calculated from the hyperspectral data. The study was performed with commercial colour standards and pen ink lines, corresponding to large and small-scale colour areas, respectively...
May 17, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28538186/an-engineered-virus-library-as-a-resource-for-the-spectrum-wide-exploration-of-virus-and-vector-diversity
#6
Wenli Zhang, Jun Fu, Jing Liu, Hailong Wang, Maren Schiwon, Sebastian Janz, Lukas Schaffarczyk, Lukas von der Goltz, Eric Ehrke-Schulz, Johannes Dörner, Manish Solanki, Philip Boehme, Thorsten Bergmann, Andre Lieber, Chris Lauber, Andreas Dahl, Andreas Petzold, Youming Zhang, A Francis Stewart, Anja Ehrhardt
Adenoviruses (Ads) are large human-pathogenic double-stranded DNA (dsDNA) viruses presenting an enormous natural diversity associated with a broad variety of diseases. However, only a small fraction of adenoviruses has been explored in basic virology and biomedical research, highlighting the need to develop robust and adaptable methodologies and resources. We developed a method for high-throughput direct cloning and engineering of adenoviral genomes from different sources utilizing advanced linear-linear homologous recombination (LLHR) and linear-circular homologous recombination (LCHR)...
May 23, 2017: Cell Reports
https://www.readbyqxmd.com/read/28537997/where-are-we-heading-with-fluid-responsiveness-research
#7
Simon T Vistisen, Peter Juhl-Olsen
PURPOSE OF REVIEW: Fluid responsiveness prediction is not always possible with well established dynamic approaches such as passive leg raising or pulse pressure variation in the ICU. The purpose of the present review is to summarize emerging alternative techniques for fluid responsiveness prediction in adult critically ill patients and discuss their methodology and applicability. In addition, the future role of fluid responsiveness prediction in the ICU is discussed. RECENT FINDINGS: Several new dynamic techniques have been investigated and they all contribute to the applicability of fluid responsiveness techniques...
May 22, 2017: Current Opinion in Critical Care
https://www.readbyqxmd.com/read/28535648/-effect-of-%C3%AE-40p53-isoform-on-enhancing-the-pro-apoptotic-function-of-p53-in-tumor-cells
#8
B S Wang, H W Zhao, L X Qiao, J Q Shan, Q S Hou, D X Chen, H L Guo
Objective: To investigate the effect of Δ40p53, an alternative spliced isoform of p53 lacking the N-ter minus, on the pro-apoptotic function of p53. Methods: The wild-type p53 was ectopically expressed in HCT116-p53(-/-) (endogenous Δ40p53 expression), HCT116-p53(+ /+) (wild-type p53) and H1299 (p53-null) cells by adenoviral delivery, while Δ40p53 plasmid were transfected into these cells to overexpress Δ40p53. The levels of Δ40p53 and p53 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) and quantitative PCR...
May 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28535455/the-effectiveness-of-telemedicine-delivered-opioid-agonist-therapy-in-a-supervised-clinical-setting
#9
Joseph K Eibl, Graham Gauthier, David Pellegrini, Jeffery Daiter, Michael Varenbut, John C Hogenbirk, David C Marsh
OBJECTIVE: Opioid use disorder has been declared a public health crisis across North America and opioid agonist therapy (OAT) is the standard of care for these patients. Despite the increasing adoption of telemedicine as a delivery method for OAT, its effectiveness has not yet been evaluated against traditional in-person treatment. This study compared treatment outcomes for in-person versus telemedicine-delivered OAT. METHODS: We conducted a non-randomized cohort comparison study using an administrative database for patients who commenced OAT between 2011 and 2012 across 58 clinic sites in the province of Ontario, Canada...
May 17, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28535451/a-novel-carbohydrate-labeling-method-utilizing-transfer-hydrogenation-mediated-reductive-amination
#10
Zsuzsanna Kovács, Gábor Papp, Henrietta Horváth, Ferenc Joó, András Guttman
One of the most frequently used high-resolution glycan analysis methods in the biopharmaceutical and biomedical fields is capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. Glycans are usually labeled by reductive amination with a charged fluorophore containing a primary amine, which reacts with the aldehyde group at the reducing end of the glycan structures. In this reaction, first a Schiff base is formed that is reduced to form a stable conjugate by a hydrogenation reagent, such as sodium cyanoborohydride...
May 13, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28535332/interventions-for-treating-anxiety-after-stroke
#11
REVIEW
Peter Knapp, C Alexia Campbell Burton, John Holmes, Jenni Murray, David Gillespie, C Elizabeth Lightbody, Caroline L Watkins, Ho-Yan Y Chun, Sharon R Lewis
BACKGROUND: Approximately 20% of stroke patients experience clinically significant levels of anxiety at some point after stroke. Physicians can treat these patients with antidepressants or other anxiety-reducing drugs, or both, or they can provide psychological therapy. This review looks at available evidence for these interventions. This is an update of the review first published in October 2011. OBJECTIVES: The primary objective was to assess the effectiveness of pharmaceutical, psychological, complementary, or alternative therapeutic interventions in treating stroke patients with anxiety disorders or symptoms...
May 23, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28534678/second-harmonic-correlation-spectroscopy-theory-and-principles-for-determining-surface-binding-kinetics
#12
Krystal L Sly, John C Conboy
A novel application of second harmonic correlation spectroscopy (SHCS) for the direct determination of molecular adsorption and desorption kinetics to a surface is discussed in detail. The surface-specific nature of second harmonic generation (SHG) provides an efficient means to determine the kinetic rates of adsorption and desorption of molecular species to an interface without interference from bulk diffusion, which is a significant limitation of fluorescence correlation spectroscopy (FCS). The underlying principles of SHCS for the determination of surface binding kinetics are presented, including the role of optical coherence and optical heterodyne mixing...
June 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28534474/influence-of-nanoscale-topology-on-bactericidal-efficiency-of-black-silicon-surfaces
#13
Denver P Linklater, Huu Khuong Duy Nguyen, Chris M Bhadra, Saulius Juodkazis, Elena P Ivanova
The nanostructuring of materials to create bactericidal and antibiofouling surfaces presents an exciting alternative to common methods of preventing bacterial adhesion. The fabrication of synthetic bactericidal surfaces has been inspired by the anti-wetting and anti-biofouling properties of insect wings, and other topologies found in nature. Black silicon is one such synthetic surfaces which has established bactericidal properties. In this study we show that time-dependent plasma etching of silicon wafers using 15, 30, and 45 min etching intervals, is able to produce different surface geometries with linearly increasing heights of approximately 280, 430, and 610 nm, respectively...
May 23, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28534417/clinical-and-cost-effectiveness-of-home-based-cardiac-rehabilitation-compared-to-conventional-centre-based-cardiac-rehabilitation-results-of-the-fit-home-study
#14
Jos J Kraal, M Elske Van den Akker-Van Marle, Ameen Abu-Hanna, Wim Stut, Niels Peek, Hareld Mc Kemps
Aim Although cardiac rehabilitation improves physical fitness after a cardiac event, many eligible patients do not participate in cardiac rehabilitation and the beneficial effects of cardiac rehabilitation are often not maintained over time. Home-based training with telemonitoring guidance could improve participation rates and enhance long-term effectiveness. Methods and results We randomised 90 low-to-moderate cardiac risk patients entering cardiac rehabilitation to three months of either home-based training with telemonitoring guidance or centre-based training...
January 1, 2017: European Journal of Preventive Cardiology
https://www.readbyqxmd.com/read/28534314/rapid-molecular-sexing-of-three-spined-sticklebacks-gasterosteus-aculeatus-l-based-on-large-y-chromosomal-insertions
#15
Theo C M Bakker, Thomas Giger, Joachim G Frommen, Carlo R Largiadèr
There is a need for rapid and reliable molecular sexing of three-spined sticklebacks, Gasterosteus aculeatus, the supermodel species for evolutionary biology. A DNA region at the 5' end of the sex-linked microsatellite Gac4202 was sequenced for the X chromosome of six females and the Y chromosome of five males from three populations. The Y chromosome contained two large insertions, which did not recombine with the phenotype of sex in a cross of 322 individuals. Genetic variation (SNPs and indels) within the insertions was smaller than on flanking DNA sequences...
May 22, 2017: Journal of Applied Genetics
https://www.readbyqxmd.com/read/28534059/cryo-mesh-a-simple-alternative-cryopreservation-protocol
#16
B Funnekotter, E Bunn, R L Mancera
BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species. Methods like the cryo-plate have proven beneficial in simplifying the cryopreservation procedure. OBJECTIVE: This study assessed the practicality of a stainless steel mesh strip (cryo-mesh) for cryopreserving shoot tips from Anigozanthos viridis. MATERIALS AND METHODS: Shoot tips of A. viridis (Kangaroo Paw) were precultured on 0...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28533990/anomalous-origin-of-right-coronary-artery-originating-from-the-pulmonary-trunk-arcapa-an-incidental-finding-in-a-patient-presenting-with-chest-pain
#17
Pragathi Balakrishna, Michael Illovsky, Youssef M Al-Saghir, Abdul M Minhas
Anomalous origin of the right coronary artery originating from the pulmonary trunk (ARCAPA) is a rare congenital coronary anomaly with an estimated prevalence of 0.002%. Most patients are asymptomatic and the anomaly is detected incidentally during evaluation for other problems. Occasionally, ARCAPA may lead to myocardial ischemia and/or sudden cardiac arrest. We present a case of a 55-year-old female with a history of hypertension who presented to the emergency department with intermittent chest discomfort for three days...
April 17, 2017: Curēus
https://www.readbyqxmd.com/read/28532968/off-loading-wheelchair-cushion-provides-best-case-reduction-in-tissue-deformation-as-indicated-by-mri
#18
Evan Call, Thomas Hetzel, Chad McLean, Joshua N Burton, Craig Oberg
Off-loading or the Orthotic approach to wheelchair seating has been used successfully to provide seating that optimizes tissue protection at the ischial tuberosities (ITs), sacrum and greater trochanters. Recent publications indicate the significance of preventing tissue compression to reduce ulcer formation. Comparative Magnetic Resonance Imaging (MRI) of individuals seated on two cushion types provides direct evidence of tissue unloading resulting in the reduction in tissue compression. Measurement of tissue compression in MRI images provides the cumulative impact of compression and shear resulting in ultimate tissue thickness documented here...
May 18, 2017: Journal of Tissue Viability
https://www.readbyqxmd.com/read/28532915/endoscopic-third-ventriculostomy-outcome-analysis-of-an-anterior-entry-point
#19
Mohammed Aref, Amanda Martyniuk, Siddharth Nath, Alex Koziarz, Jetan Badhiwala, Almunder Algird, Forough Farrokhyar, Saleh A Almenawer, Kesava Reddy
BACKGROUND: Endoscopic third ventriculostomy (ETV) is a safe and effective treatment for hydrocephalus. An entry point located 4 cm anterior to the coronal suture, 3 cm anterior to Kocher's point, and approximately 9 cm from the pupil at the mid-pupillary line, has been used successfully for the past 20 years in our center. We aimed to evaluate this alternative anterior entry point routinely used for ETV, with or without concurrent endoscopic biopsy. METHODS: Patients undergoing this proposed entry point were examined to evaluate its safety and efficacy...
May 19, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28532839/dry-ice-is-a-reliable-substrate-for-the-distribution-of-frozen-mouse-spermatozoa-a-multi-centric-study
#20
Marcello Raspa, Mo Guan, Renata Paoletti, Lluis Montoliu, Abdel Ayadi, Susan Marschall, Martin Fray, Ferdinando Scavizzi
Disseminating mouse stocks as frozen materials offers both ethical and logistical advantages over live animal shipment, minimizing the welfare issues and avoiding some of the complex custom regulations that are associated with live animal transportation. Embryo freezing in liquid nitrogen (LN2) at -196 °C has traditionally been the method of choice for archiving mouse lines. However, spermatozoa freezing is emerging as a more convenient alternative due to the application of innovative cryopreservation and recovery protocols...
July 1, 2017: Theriogenology
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