Mitra Shokrollahi, Mia Stanic, Anisha Hundal, Janet N Y Chan, Defne Urman, Chris A Jordan, Anne Hakem, Roderic Espin, Jun Hao, Rehna Krishnan, Philipp G Maass, Brendan C Dickson, Manoor P Hande, Miquel A Pujana, Razqallah Hakem, Karim Mekhail
Current models suggest that DNA double-strand breaks (DSBs) can move to the nuclear periphery for repair. It is unclear to what extent human DSBs display such repositioning. Here we show that the human nuclear envelope localizes to DSBs in a manner depending on DNA damage response (DDR) kinases and cytoplasmic microtubules acetylated by α-tubulin acetyltransferase-1 (ATAT1). These factors collaborate with the linker of nucleoskeleton and cytoskeleton complex (LINC), nuclear pore complex (NPC) protein NUP153, nuclear lamina and kinesins KIF5B and KIF13B to generate DSB-capturing nuclear envelope tubules (dsbNETs)...
April 17, 2024: Nature Structural & Molecular Biology