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Brian D Johnston, Paul Thuras, James R Johnson
BACKGROUND: Escherichia coli sequence type 131 (ST131), with its multidrug-resistance-associated H30R1 and H30Rx clonal subsets, causes most antimicrobial-resistant E. coli infections in the U.S., especially among veterans. The activity of ceftolozane-tazobactam (C/T), a new beta-lactamase inhibitor agent, against ST131 strains, and E. coli isolates from veterans, is undefined. METHODS: We determined broth microdilution MICs for C/T and five comparators-piperacillin-tazobactam (TZP) levofloxacin (LVX), gentamicin (GEN), ceftazidime (CAZ), and meropenem (MEM)-for 595 clinical E...
2018: PloS One
Amit Ranjan, Julia Scholz, Torsten Semmler, Lothar H Wieler, Christa Ewers, Stefanie Müller, Derek J Pickard, Peter Schierack, Karsten Tedin, Niyaz Ahmed, Katharina Schaufler, Sebastian Guenther
Background: Extended spectrum beta lactamase (ESBL)-producing extraintestinal pathogenic Escherichia coli infections are of global interest because of their clinical and economic impact. The ESBL resistance genes disseminate through plasmids, and are found in successful global lineages such as ST131 and ST648. The carriage of plasmids has been suggested to result in a fitness burden, but recently it was shown that ESBL-plasmids enhanced virulence in pandemic ST131 and ST648 lineages without affecting their fitness...
2018: Gut Pathogens
Bradley W Frazee, Tarak Trivedi, Martha Montgomery, Danka-Florence Petrovic, Reina Yamaji, Lee Riley
STUDY OBJECTIVE: Community-onset urinary tract infections (UTIs) caused by extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, which are resistant to ceftriaxone and usually coresistant to fluoroquinolones, are increasing worldwide. We investigate and describe in detail UTIs caused by ESBL-producing Enterobacteriaceae in our emergency department (ED), and determine the proportion that occurred in patients without health care-associated risk factors and who received discordant initial antibiotic therapy...
July 3, 2018: Annals of Emergency Medicine
Tolbert Sonda, Happiness Kumburu, Marco van Zwetselaar, Michael Alifrangis, Blandina T Mmbaga, Frank M Aarestrup, Gibson Kibiki, Ole Lund
Background: Limited information regarding the clonality of circulating E. coli strains in tertiary care hospitals in low and middle-income countries is available. The purpose of this study was to determine the serotypes, antimicrobial resistance and virulence genes. Further, we carried out a phylogenetic tree reconstruction to determine relatedness of E. coli isolated from patients in a tertiary care hospital in Tanzania. Methods: E. coli isolates from inpatients admitted at Kilimanjaro Christian Medical Centre between August 2013 and August 2015 were fully genome-sequenced at KCMC hospital...
2018: Antimicrobial Resistance and Infection Control
Veronika Tchesnokova, Kim Riddell, Delia Scholes, James R Johnson, Evgeni V Sokurenko
Background: The pandemic spread of antibiotic resistance increases the likelihood of ineffective empirical therapy. The recently emerged fluoroquinolone-resistant Escherichia coli ST131-H30R subclone (H30) is a leading cause of multidrug-resistant urinary tract infection (UTI) and bloodstream infection worldwide. Objective: We studied the relative impact of H30 on the likelihood that bacteria isolated from urine of urgent care patients would be resistant to the empirically prescribed antibiotic regimen for UTI...
June 29, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Ji Young Park, Ki Wook Yun, Eun Hwa Choi, Hoan Jong Lee
Escherichia coli sequence type (ST) 131 has emerged as a higher virulent and multidrug-resistant pathogen worldwide. This study aimed to identify the prevalence and characteristics of E. coli ST131 isolated from Korean children with bacteremia at a single center for 16 years. We retrospectively reviewed culture-proven E. coli bacteremia cases of children aged ≤18 years between 2000 and 2015. E. coli isolates were analyzed using multilocus sequence typing, fimH typing, and CTX-M typing. Among 177 children with E...
June 29, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Cosmika Goswami, Stephen Fox, Matthew Holden, Martin Connor, Alistair Leanord, Thomas J Evans
Bacteraemia caused by Escherichia coli is a growing problem with a significant mortality. The factors that influence the acquisition and outcome of these infections are not clear. Here, we have linked detailed genetic data from the whole-genome sequencing of 162 bacteraemic isolates collected in Scotland, UK, in 2013-2015, with clinical data in order to delineate bacterial and host factors that influence the acquisition in hospital or the community, outcome and antibiotic resistance. We identified four major sequence types (STs) in these isolates: ST131, ST69, ST73 and ST95...
June 22, 2018: Microbial Genomics
Toyotaka Sato, Tsukasa Shiraishi, Yoshiki Hiyama, Hiroyuki Honda, Masaaki Shinagawa, Masaru Usui, Koji Kuronuma, Naoya Masumori, Satoshi Takahashi, Yutaka Tamura, Shin-Ichi Yokota
Colistin is a last-line drug for multidrug-resistant Gram-negative bacteria. We previously reported four plasmid-mediated colistin resistance ( mcr ) gene-negative colistin-resistant Escherichia coli clinical isolates, including the major pathogenic and fluoroquinolone-resistant strains O25b:H4-ST131- H 30Rx [isolates SRE34 and SRE44, minimum inhibitory concentration (MIC) for colistin = 16 mg/L] and non-x (SME296, MIC = 8 mg/L), and O18-ST416 (SME222, MIC = 4 mg/L). Herein, we investigated the colistin resistance mechanism, and identified novel amino acid substitutions or deletion in the PmrAB two-component system that activates eptA (encoding a phosphoethanolamine transferase) and arnT (encoding an undecaprenyl phosphate-alpha-4-amino-4-deoxy-l-arabinose arabinosyl transferase) in all colistin-resistant isolates...
June 18, 2018: Antimicrobial Agents and Chemotherapy
I-Fei Huang, Wei-Yang Lee, Jiun-Ling Wang, Chih-Hsin Hung, Hong-Hsiang Hu, Wan-Yu Hung, Yun-Ju Hung, Wen-Chi Chen, Ying-Tso Shen, Ming-Fang Cheng
BACKGROUND: The emergence of multidrug-resistant (MDR) Escherichia coli (E. coli), particularly E. coli sequence type ST131, is becoming a global concern. Commensal bacteria, an important reservoir of antibiotic resistance genes, facilitate the spread of such genes to pathogenic bacterial strains. The objective of the study is to investigate the fecal carriage of MDR E. coli and ST131 E. coli in community children in Southern Taiwan. METHODS: In this prospective study, stool samples from children aged 0-18 years were obtained within 3 days of hospitalization from October 2013 to September 2014...
June 15, 2018: BMC Gastroenterology
Sameer Ahmed Alsharapy, Betitera Yanat, Lorena Lopez-Cerero, Samar Saeed Nasher, Paula Díaz-De-Alba, Álvaro Pascual, José-Manuel Rodríguez-Martínez, Abdelaziz Touati
OBJECTIVE: The aim of this study was to characterize the O25b/ST131 clone in ciprofloxacin-resistant Escherichia coli isolates from Yemen. MATERIALS AND METHODS: A total of 41 ciprofloxacin-resistant E. coli strains were collected from clinical samples of inpatients and outpatients from Sana'a (Yemen) from January to December 2013. Antimicrobial susceptibility testing, polymerase chain reaction amplification, and sequencing were used for detection of plasmid-mediated quinolone resistance determinants, extended-spectrum beta-lactamases genes and mutations in the quinolone resistance-determining regions of the target genes gyrA and parC...
June 8, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Bertha Rusdi, Tanya Laird, Rebecca Abraham, Amanda Ash, Ian D Robertson, Shewli Mukerji, Geoffrey W Coombs, Sam Abraham, Mark A O'Dea
Camp dogs in indigenous communities in the Western Australian Kimberley Region, share the domestic environment with humans and have the potential to act as carriers of, and sentinels for, a wide range of zoonotic agents, including intestinal parasites and antimicrobial resistant bacteria. In this study, we investigated the carriage of extended-spectrum-cephalosporin-resistant (ESC-resistant) Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA) and species of hookworm and Giardia among camp dogs in remote Western Australian Aboriginal communities...
June 7, 2018: Scientific Reports
Jeremiah Seni, Giselle Peirano, Kenneth Okwong Okon, Yusuf Bara Jibrin, Alkali Mohammed, Stephen E Mshana, Rebekah DeVinney, Johann D D Pitout
Limited information is available regarding the population structure of extra-intestinal pathogenic Escherichia coli (ExPEC) in Africa. Antimicrobial resistance profiles, sequence types (STs) and fimH types were determined on 60 clinical ExPEC from Nigeria using a 7-single nucleotide polymorphism quantitative PCR and sequencing of certain genes. Different ST131 clades were identified with a multiplex PCR. The isolates were mostly obtained from urines (58.3%). Not-susceptibility rates were as follows: trimethoprim-sulfamethoxazole (98%), cefotaxime (68%), gentamicin (55%), ciprofloxacin (62%) and piperacillin-tazobactam (2%)...
April 14, 2018: Diagnostic Microbiology and Infectious Disease
Young Ah Kim, Kyungwon Lee, Jae Eun Chung
BACKGROUND: Escherichia coli (E. coli) is known to cause urinary tract infection (UTI) and meningitis in neonates, as well as existing as a commensal flora of the human gut. Extended-spectrum β-lactamase (ESBL)-producing E. coli has increased in the community with the spread of CTX-M type ESBL-producing sequence type 131 (ST131)-O25-H30Rx E. coli clone. The role of ESBL-producing E. coli in female genital tract infection has not been elucidated. The clinical and molecular features of E...
June 1, 2018: BMC Infectious Diseases
Marzie Moghanni, Kiarash Ghazvini, Hadi Farsiani, Mohammad Hasan Namaei, Mohammad Derakhshan, Masoud Yousefi, Alimohammad Maragheh, Saeid Amel Jamehdar
OBJECTIVES: The recent expansion of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli (E. coli) is a worldwide problem. The purpose of this study was to investigate the molecular characteristic of ESBL-producing E. coli strains in Mashhad, located in the northeast of Iran. METHODS: A total of 455 clinical isolates of E. coli were collected at three hospitals in Mashhad between April and September 2015. Antibiotic susceptibility was determined with the Kirby Bauer disk diffusion test...
May 25, 2018: Journal of Global Antimicrobial Resistance
Apostolos Liakopoulos, Jonathan Betts, Roberto La Ragione, Alieda van Essen-Zandbergen, Daniela Ceccarelli, Efthymia Petinaki, Christos K Koutinas, Dik J Mevius
Extended-spectrum cephalosporin- and/or carbapenem-resistant (ESCR and/or CarbR ) Enterobacteriaceae constitute a public health hazard because of limited treatment options and are endemic among humans in Greece. Recently, ESCR and CarbR Enterobacteriaceae have been increasingly isolated from companion animals, stressing their potential role as a reservoir for humans. However, the presence of ESCR bacteria in companion animals within Greek households has not been determined yet. Genes conferring the ESCR and CarbR phenotype were detected among canine isolates and their chromosomal or plasmid location was determined...
May 25, 2018: Journal of Medical Microbiology
Kumiko Kawamura, Kengo Hayashi, Nao Matsuo, Kazuki Kitaoka, Kouji Kimura, Jun-Ichi Wachino, Takaaki Kondo, Yoshitsugu Iinuma, Nobuo Murakami, Shuhei Fujimoto, Yoshichika Arakawa
We investigated the prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli among 258 residents of long-term care facilities (LTCFs) in Japan. Out of 258 fecal samples collected from nine LTCFs between November 2015 and March 2017, we recovered 59 ESBL-producing E. coli isolates. All isolates carried blaCTX-M genes, mainly blaCTX-M-27 (42.4%), blaCTX-M-14 (23.7%), and blaCTX-M-15 (18.6%). The isolates showed 7 serotypes (STs), including ST131 (n = 49, 83.1%) and ST38 (n = 4, 6...
May 23, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Luis M Guachalla, Katharina Ramoni, Cecilia Varga, Michele Mutti, Akela Ghazawi, Tibor Pál, Eszter Nagy, Ágnes Sonnevend, Gábor Nagy, Valéria Szijártó
Plasmid-encoded colistin resistance is emerging among extra-intestinal pathogenic Escherichia coli including the epidemic clone ST131-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of LPS conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody, ASN-4 specific to the O25b side-chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin neutralizing activity and therefore offer a treatment option against extremely drug-resistant ST131 isolates...
April 23, 2018: Antimicrobial Agents and Chemotherapy
Alejandra Aquino-Andrade, Jocelin Merida-Vieyra, Eduardo Arias de la Garza, Patricia Arzate-Barbosa, Agustín De Colsa Ranero
BACKGROUND: Carbapenemases-producing Enterobacteriaceae (CPE) are a worldwide public health emergency. In Mexico, reports of CPE are limited, particularly in the pediatric population. Here, we describe the clinical, epidemiological, and molecular characteristics of seven consecutive cases in a third-level pediatric hospital in Mexico City over a four-month period during 2016. RESULTS: The Enterobacteriaceae identified were three Escherichia coli strains (producing OXA-232, NDM-1 and KPC-2), two Klebsiella pneumoniae strains (producing KPC-2 and NDM-1), one Klebsiella oxytoca strain producing OXA-48 and one Enterobacter cloacae strain producing NDM-1...
April 19, 2018: BMC Microbiology
Anna Lena Zogg, Sabrina Simmen, Katrin Zurfluh, Roger Stephan, Sarah N Schmitt, Magdalena Nüesch-Inderbinen
Objectives: This study aimed to identify and characterize extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae among clinical samples of companion animals. Methods: A total of 346 non-duplicate Enterobacteriaceae isolates were collected between 2012 and 2016 from diseased cats ( n  = 115) and dogs ( n  = 231). The presence of bla ESBL , PMQR genes, and the azithromycin resistance gene mph(A) was confirmed by PCR and sequencing of bla genes...
2018: Frontiers in Veterinary Science
Rabaab Zahra, Saba Javeed, Bibi Malala, Dmitriy Babenko, Mark A Toleman
Objectives: To discover the Escherichia coli STs and associated resistance mechanisms in the community in Islamabad, Pakistan by analysis of E. coli isolates in sewage. Methods: One hundred and ten E. coli were isolated from sewage across the city of Islamabad without antibiotic bias and confirmed as E. coli by MALDI-TOF MS. Isolates were characterized by fumC/fimH (CH) typing and core-genome MLST. Resistance mechanisms, virulence genes, phylotypes and plasmid incompatibility types were determined in a subset of isolates by in silico analysis...
April 10, 2018: Journal of Antimicrobial Chemotherapy
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