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Jason W Sahl, Mark Mayo, Erin P Price, Derek S Sarovich, Mirjam Kaestli, Talima Pearson, Charles H D Williamson, Roxanne Nottingham, Krystal Sheridan, David M Wagner, Bart J Currie, Paul Keim
The Burkholderia pseudomallei isolate MSHR1435 is a fully virulent environmental sequence type 131 (ST131) isolate that is epidemiologically associated with a 17.5-year chronic melioidosis infection. The completed genome will serve as a reference for studies of environmental ecology, virulence, and chronic B. pseudomallei infections.
March 15, 2018: Genome Announcements
Anna Lena Zogg, Katrin Zurfluh, Sarah Schmitt, Magdalena Nüesch-Inderbinen, Roger Stephan
Among 64 uropathogenic Escherichia coli (UPEC) isolated from 13 cats and 51 dogs, 35 were extended-spectrum beta-lactamase (ESBL) producers, and 29 were non-ESBL producers. Forty-six (71.9%) of the isolates were multidrug resistant (MDR). Among the ESBL producers, blaCTX-M-15 (n = 17/48.6% of the blaESBLs ), blaCTX-M-1 (n = 10/28.6%), blaCTX-M-55 (n = 4/11.4%), blaCTX-M-14 (n = 3/8.6%), and blaCTX-M-27 (n = 1/2.9%) were identified. The plasmid-mediated fluoroquinolone resistance genes aac(6')-Ib-cr, qnrB and the azithromycin resistance gene mph(A) were detected in 17 (26...
March 2018: Veterinary Microbiology
Yoshihiko Maeyama, Yui Taniguchi, Wataru Hayashi, Yusuke Ohsaki, Shunsuke Osaka, Shota Koide, Kiyoko Tamai, Yukiko Nagano, Yoshichika Arakawa, Noriyuki Nagano
In recent years, besides the widespread occurrence of extended-spectrum β-lactamase (ESBL)- and/or plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae in both healthcare and community settings of humans, the third-generation cephalosporin (3GC)-resistant microbes have also been reported from companion animals worldwide. Here, we characterized ESBL- and/or pAmpC-producing Enterobacteriaceae clinical isolates from companion animals. Among the 487 clinical isolates mainly from urine of dogs and cats between May and September 2016, 104 non-repetitive isolates were resistant to the 3GC, and they consisted of 81 of 381 (21...
March 2018: Veterinary Microbiology
Apostolos Liakopoulos, Gerrita van den Bunt, Yvon Geurts, Martin C J Bootsma, Mark Toleman, Daniela Ceccarelli, Wilfrid van Pelt, Dik J Mevius
Extended-spectrum cephalosporin-resistant (ESCR ) Enterobacteriaceae pose a serious infection control challenge for public health. The emergence of the ESCR phenotype is mostly facilitated by plasmid-mediated horizontal extended-spectrum β-lactamases (ESBLs) and AmpC gene transfer within Enterobacteriaceae . Current data regarding the plasmid contribution to this emergence within the Dutch human population is limited. Hence, the aim of this study was to gain insight into the role of plasmids in the dissemination of ESBL/AmpC genes inside Dutch households with preschool children and precisely delineate co-colonization...
2018: Frontiers in Microbiology
Ana Carolina C Campos, Nathália L Andrade, Mithila Ferdous, Monika A Chlebowicz, Carla C Santos, Julio C D Correal, Jerome R Lo Ten Foe, Ana Cláudia P Rosa, Paulo V Damasco, Alex W Friedrich, John W A Rossen
Urinary tract infections (UTIs) are often caused by Escherichia coli . Their increasing resistance to broad-spectrum antibiotics challenges the treatment of UTIs. Whereas, E. coli ST131 is often multidrug resistant (MDR), ST69 remains susceptible to antibiotics such as cephalosporins. Both STs are commonly linked to community and nosocomial infections. E. coli phylogenetic groups B2 and D are associated with virulence and resistance profiles making them more pathogenic. Little is known about the population structure of E...
2018: Frontiers in Microbiology
Aurélie Jayol, Camille Corlouer, Marisa Haenni, Mélanie Darty, Karine Maillard, Marine Desroches, Brigitte Lamy, Estelle Jumas-Bilak, Jean-Yves Madec, Jean-Winoc Decousser
Stenotrophomonas maltophilia (Sm) is an archetypal environmental opportunistic bacterium responsible for health care-associated infections. The role of animals in human Sm infections is unknown. This study aims to reveal the genetic and phylogenetic relationships between pathogenic strains of Sm, both animal and human, and identify a putative role for animals as a reservoir in human infection. We phenotypically and genotypically characterized 61 Sm strains responsible for animal infections (mainly respiratory tract infections in horses) from a French nationwide veterinary laboratory network...
February 27, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Michele Mutti, Ágnes Sonnevend, Tibor Pál, Sini Junttila, Heinz Ekker, Bence Galik, Attila Gyenesei, Gábor Nagy, Eszter Nagy, Valéria Szijártó
The sequence type 131 (ST131)- H 30 clone is responsible for a significant proportion of multidrug-resistant extraintestinal Escherichia coli infections. Recently, the C1-M27 clade of ST131- H 30, associated with bla CTX-M-27 , has emerged. The complete genome sequence of E. coli isolate 81009 belonging to this clone, previously used during the development of ST131-specific monoclonal antibodies, is reported here.
February 22, 2018: Genome Announcements
Sohinee Sarkar, Melanie L Hutton, Dimitrios Vagenas, Rinaldo Ruter, Stephanie Schüller, Dena Lyras, Mark A Schembri, Makrina Totsika
Background: Epidemiological studies point to the gut as a key reservoir of multidrug resistant Escherichia coli multilocus sequence type 131 (ST131), a globally dominant pathogenic clone causing urinary tract and bloodstream infections. Here we report a detailed investigation of its intestinal lifestyle. Methods: Clinical ST131 isolates and type 1 fimbriae null mutants were assessed for colonization of human intestinal epithelia and in mouse intestinal colonization models...
February 16, 2018: Journal of Infectious Diseases
Yacoub R Nairoukh, Azmi M Mahafzah, Amal Irshaid, Asem A Shehabi
Background: Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan. Methods: A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method...
2018: Open Microbiology Journal
Reina Yamaji, Julia Rubin, Erika Thys, Cindy R Friedman, Lee W Riley
BACKGROUND: Incidence of drug-resistant community-acquired urinary tract infections (CA-UTI) continues to increase worldwide. In 1999-2000, a single lineage of uropathogenic Escherichia coli (UPEC) ST69 caused 51% of trimethoprim-sulfamethoxazole-resistant UTI in a Northern California university community. We compared the clonal distribution of UPEC and its impact on antimicrobial resistance prevalence in the same community during two periods separated by 17 years. METHODS: We analyzed E...
February 7, 2018: Journal of Clinical Microbiology
Ana Isabel Vitas, Dixita Naik, Lara Pérez-Etayo, David González
The aim of this study was to determine the occurrence and patterns of resistance of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food products purchased in Navarra, northern Spain. A total of 174 samples of fish and chicken were analyzed from September 2015 to September 2016, including raw and ready-to-eat products: trout (n = 25), salmon (n = 28), panga (n = 13), chicken nuggets and chicken scalopes (n = 32), sushi (n = 31) and sliced cooked poultry (n = 45)...
February 1, 2018: International Journal of Food Microbiology
Eunyoung Lee, Yangsoon Lee
Escherichia coli can harbor genomic pks islands that code for a polyketide-peptide genotoxin known as colibactin. E. coli strains carrying pks islands trigger genetic instability. pks islands have been significantly associated with bacteremia. We investigated the molecular epidemiology of bacteremic E. coli isolates and the prevalence of bacteremia-causing E. coli carrying pks islands. A total of 146 E. coli isolates were collected at a tertiary-care hospital from January 2015 to December 2016. The phylogenetic groups were determined by multiplex PCR...
May 2018: Annals of Laboratory Medicine
Jessica Joerling, Stefanie A Barth, Karen Schlez, Hermann Willems, Werner Herbst, Christa Ewers
Swine dysentery (SD) is an economically important diarrheal disease in pigs caused by different strongly hemolytic Brachyspira (B.) species, such as B. hyodysenteriae, B. suanatina and B. hampsonii. Possible associations of epidemiologic data, such as multilocus sequence types (STs) to virulence gene profiles and antimicrobial susceptibility are rather scarce, particularly for B. hyodysenteriae isolates from Germany. In this study, B. hyodysenteriae (n = 116) isolated from diarrheic pigs between 1990 and 2016 in Germany were investigated for their STs, susceptibility to the major drugs used for treatment of SD (tiamulin and valnemulin) and genes that were previously linked with virulence and encode for hemolysins (tlyA, tlyB, tlyC, hlyA, BHWA1_RS02885, BHWA1_RS09085, BHWA1_RS04705, and BHWA1_RS02195), outer membrane proteins (OMPs) (bhlp16, bhlp17...
2018: PloS One
Chihiro Norizuki, Kumiko Kawamura, Jun-Ichi Wachino, Masahiro Suzuki, Noriyuki Nagano, Takaaki Kondo, Yoshichika Arakawa
We investigated the prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from Japanese pigs. A total of 345 pig fecal specimens were collected from 30 farms in Aichi prefecture, Japan, between June 2015 and April 2016, and 22 unique ESBL-producing E. coli were isolated from 16 samples of eight farms. The ESBL types included CTX-M-15 (54.5%), CTX-M-55 (27.2%), CTX-M-3 (0.9%), and CTX-M-14 (0.9%). The predominant plasmid replicon type was IncN, and they carried blaCTX-M-55...
December 26, 2017: Japanese Journal of Infectious Diseases
Miriam D Ismail, Ihsan Ali, Savannah Hatt, Elizabeth A Salzman, Anna W Cronenwett, Carl F Marrs, Alexander H Rickard, Betsy Foxman
OBJECTIVES: Test the hypothesis that urinary tract infections (UTI) caused by Escherichia coli of the ST131 lineage are more likely to recur than UTI cause by other E. coli lineages. METHODS: We used isolates from 221 young women with UTI caused by Escherichia coli participating in a randomized controlled trial. Participants were followed for 6 months or until recurrence. RESULTS: Sequence type was not associated with risk of recurrence. Isolates in the ST131 lineage were more resistant to quinolones (6% vs 1%) than other STs, but not trimethoprim sulfamethoxazole (15% vs 15%)...
December 16, 2017: Journal of Global Antimicrobial Resistance
Patrick N A Harris, Nouri L Ben Zakour, Leah W Roberts, Alexander M Wailan, Hosam M Zowawi, Paul A Tambyah, David C Lye, Roland Jureen, Tau H Lee, Mo Yin, Ezlyn Izharuddin, David Looke, Naomi Runnegar, Benjamin Rogers, Hasan Bhally, Amy Crowe, Mark A Schembri, Scott A Beatson, David L Paterson
Objectives: To characterize MDR Escherichia coli from bloodstream infections (BSIs) in Australia, New Zealand and Singapore. Methods: We collected third-generation cephalosporin-resistant (3GC-R) E. coli from blood cultures in patients enrolled in a randomized controlled trial from February 2014 to August 2015. WGS was used to characterize antibiotic resistance genes, MLST, plasmids and phylogenetic relationships. Antibiotic susceptibility was determined using disc diffusion and Etest...
December 14, 2017: Journal of Antimicrobial Chemotherapy
Magdalena T Nüesch-Inderbinen, Melinda Baschera, Katrin Zurfluh, Herbert Hächler, Hansjakob Nüesch, Roger Stephan
Objectives: The aim of this study was to assess the clonal structure, virulence potential and antibiotic susceptibility of uropathogenic Escherichia coli (UPEC) isolates causing community acquired urinary tract infection (CAUTI) in unselected primary care patients in Switzerland. Methods: We performed multilocus sequence typing, virulence factor determination, and phenotypic and genotypic antimicrobial resistance testing on 44 non-duplicate UPEC isolates. Results: Twenty-seven different sequence types (STs) were identified...
2017: Frontiers in Microbiology
Denise De Belder, Celeste Lucero, Melina Rapoport, Adriana Rosato, Diego Faccone, Alejandro Petroni, Fernando Pasteran, Ezequiel Albornoz, Alejandra Corso, Sonia A Gomez
The predominance of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae was caused by the spread of ST258 clone. In Latin America, KPC was reported in 2006, with the isolation of genetically unrelated K. pneumoniae in Colombia. Since then, the expansion of blaKPC in either K. pneumoniae ST258 or other Enterobacteriaceae (ETB) species was increasingly reported. In this study, we characterized 89 KPC-producing Escherichia coli, Klebsiella oxytoca, Serratia marcescens, and Citrobacter freundii that were received between 2010 and 2014...
December 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Øyvind Andreas Fladberg, Silje Bakken Jørgensen, Hege Vangstein Aamot
Background: Cephalosporin resistance in clinical E. coli isolates is increasing internationally. The increase has been caused by virulent and often multidrug-resistant clones, especially the extended spectrum β-lactamase (ESBL) producing E. coli clone O25b-ST131. Methods: In Norway, recommended empirical treatment of sepsis consists of gentamicin and penicillin combined, or a broad-spectrum cephalosporin. To investigate if increased gentamicin and cephalosporins resistance rates in our hospital could be caused by specific clones, we conducted a retrospective study on E...
2017: Antimicrobial Resistance and Infection Control
Jing Wu, Fangjun Lan, Yanfang Lu, Qingwen He, Bin Li
Objectives: Sequence type 1193 is emerging as a new, virulent and resistant lineage among fluoroquinolone resistant Escherichia coli (FQr E. coli ). In this study, we investigated the prevalence and molecular characteristics of this clone isolated from a Chinese university hospital. Methods: 73 phylogenetic group B2-FQr -non-ST131 isolates were collected from August 2014 and August 2015 at a Chinese university hospital. Isolates were screened for ST1193 by multilocus sequence typing. E. coli ST1193 then underwent lactose fermentation determination, susceptibility testing, virulence genotyping, PCR-based O typing, pulsed-field gel electrophoresis (PFGE) and FQr mechanism analysis...
2017: Frontiers in Microbiology
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