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Yasufumi Matsumura, Johann D D Pitout, Ryota Gomi, Tomonari Matsuda, Taro Noguchi, Masaki Yamamoto, Gisele Peirano, Rebekah DeVinney, Patricia A Bradford, Mary R Motyl, Michio Tanaka, Miki Nagao, Shunji Takakura, Satoshi Ichiyama
The Escherichia coli sequence type (ST) 131 C2/H30Rx clade with the blaCTX-M-15 gene had been most responsible for the global dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. ST131 C1/H30R with blaCTX-M-27 emerged among ESBL-producing E. coli in Japan during the late 2000s. To investigate the possible expansion of a single clade, we performed whole-genome sequencing for 43 Japan and 10 global ST131 isolates with blaCTX-M-27 (n = 16), blaCTX-M-14 (n = 16), blaCTX-M-15 (n = 13), and others (n = 8)...
November 2016: Emerging Infectious Diseases
Richard Aschbacher, Elisabetta Pagani, Massimo Confalonieri, Claudio Farina, Paolo Fazii, Francesco Luzzaro, Pier Giorgio Montanera, Aurora Piazza, Laura Pagani
BACKGROUND: Rates of colonization and infection with multidrug-resistant (MDR) bacteria are increasing worldwide, in both acute care hospitals and long-term care facilities (LTCFs). Italy has one of the highest prevalence of MDR bacteria in European countries, especially with regard to methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase (ESBL) or carbapenemase producing Enterobacteriaceae (CPE). METHOD: Review of studies on colonization by MDR bacteria from Italian LTCFs, risk factors for colonization and molecular characteristics of surveillance and clinical isolates, compared with other European countries...
2016: Antimicrobial Resistance and Infection Control
Dimitri M Drekonja, Michael A Kuskowski, Ruth Anway, Brian D Johnston, James R Johnson
Background.  Antimicrobial resistance among Escherichia coli is increasing, driven largely by the global emergence of sequence type 131 (ST131). However, the clinical significance of ST131 status is unknown. Among veterans, we assessed whether ST131 causes more severe, persistent, or recurrence-prone infections than non-ST131 E. coli. Methods.  Isolates were assessed by polymerase chain reaction for membership in ST131 and relevant subclones thereof (H30R and H30Rx) and by broth microdilution for susceptibility to 11 antibiotics...
September 2016: Open Forum Infectious Diseases
James R Johnson, Gregg Davis, Connie Clabots, Brian D Johnston, Stephen Porter, Chitrita DebRoy, William Pomputius, Peter T Ender, Michael Cooperstock, Billie Savvas Slater, Ritu Banerjee, Sybille Miller, Dagmara Kisiela, Evgeni V Sokurenko, Maliha Aziz, Lance B Price
Background.  Within-household sharing of strains from the resistance-associated H30R1 and H30Rx subclones of Escherichia coli sequence type 131 (ST131) has been inferred based on conventional typing data, but it has been assessed minimally using whole genome sequence (WGS) analysis. Methods.  Thirty-three clinical and fecal isolates of ST131-H30R1 and ST131-H30Rx, from 20 humans and pets in 6 households, underwent WGS analysis for comparison with 52 published ST131 genomes. Phylogenetic relationships were inferred using a bootstrapped maximum likelihood tree based on core genome sequence polymorphisms...
September 2016: Open Forum Infectious Diseases
Youjin Cheong, Young Jin Kim, Heeyoon Kang, Samjin Choi, Hee Joo Lee
We introduce a label-free spectroscopic method to classify subtypes of quinolone-nonsusceptible Escherichia coli (E. coli) isolates obtained from human blood cultures. Raman spectroscopy with a 30-nm gold-deposited, surface-enhanced Raman scattering (SERS) substrate was used to evaluate three multilocus sequencing typing (MLST)-predefined groups including E. coli ATCC25922, E. coli ST131:O75, and E. coli ST1193:O25b. Although there was a coffee-ring effect, the ring zone was selected at the ideal position to screen E...
October 2, 2016: Microscopy Research and Technique
Eyitayo O Adenipekun, Charlene R Jackson, Hazem Ramadan, Bamidele A Iwalokun, Kolawole S Oyedeji, Jonathan G Frye, John B Barrett, Lari M Hiott, Tiffanie A Woodley, Afolabi Oluwadun
INTRODUCTION: The emergence of multidrug resistance (MDR; resistance to ≥ 2 more antimicrobials) in Escherichia coli is of concern due to complications encountered in treatment. METHODOLOGY: In this study, prevalence, antimicrobial resistance, and genetic characteristics of MDR community isolates of E. coli from Lagos, Nigeria were determined. Urine and stool samples were obtained from outpatients attending Lagos State hospitals and from animal handlers in abattoirs, poultries, and open markets, from December 2012 to July 2013...
September 30, 2016: Journal of Infection in Developing Countries
Kin Chan, Clifford Shone, J Richard Hesp
PURPOSE: The focus of this study was to characterise the effect of clinically relevant stress inducing conditions on the production and composition of proinflammatory outer membrane vesicles (OMVs) produced from ST131 ExPEC clinical isolates. EXPERIMENTAL DESIGN: A label-free method (SINQ) was used to identify changes in the respective OMV proteomes following exposure of the ExPEC strains to antibiotics and low iron. Nanoparticle tracking analysis was used to quantify changes in abundance and size of OMVs produced by the gentamicin resistant (GenR) and sensitive (GenS) ExPEC strains...
September 26, 2016: Proteomics. Clinical Applications
Daria Van Tyne, Joseph B Ciolino, Jay Wang, Marlene L Durand, Michael S Gilmore
Importance: Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are highly antibiotic resistant, and primary ocular infection by ESBL E coli has rarely been reported. A novel mutation conferring phagocytosis resistance would position a strain well to infect the cornea. Observations: A woman with recurrent keratitis presented with a corneal ulcer, which was culture positive for ESBL E coli. Resistant to nearly all other antimicrobials, the infection was treated with amikacin and polymyxin B-trimethoprim, and the ulcer resolved over 3 weeks...
September 15, 2016: JAMA Ophthalmology
Alan McNally, Yaara Oren, Darren Kelly, Ben Pascoe, Steven Dunn, Tristan Sreecharan, Minna Vehkala, Niko Välimäki, Michael B Prentice, Amgad Ashour, Oren Avram, Tal Pupko, Ulrich Dobrindt, Ivan Literak, Sebastian Guenther, Katharina Schaufler, Lothar H Wieler, Zong Zhiyong, Samuel K Sheppard, James O McInerney, Jukka Corander
The use of whole-genome phylogenetic analysis has revolutionized our understanding of the evolution and spread of many important bacterial pathogens due to the high resolution view it provides. However, the majority of such analyses do not consider the potential role of accessory genes when inferring evolutionary trajectories. Moreover, the recently discovered importance of the switching of gene regulatory elements suggests that an exhaustive analysis, combining information from core and accessory genes with regulatory elements could provide unparalleled detail of the evolution of a bacterial population...
September 2016: PLoS Genetics
Ya Wang, Wai-U Lo, Raymond Wai-Man Lai, Cindy Wing-Sze Tse, Rodney Allan Lee, Wei-Kwang Luk, Vincent Chi-Chung Cheng, Tak-Lun Que, Kin-Hung Chow, Pak-Leung Ho
OBJECTIVES: To characterize blaIMP-4-carrying plasmids originating from inpatients in Hong Kong. METHODS: Sixteen blaIMP-4-carrying plasmids identified among Enterobacteriaceae (nine Escherichia coli, four Klebsiella pneumoniae, two Citrobacter freundii and one Enterobacter cloacae) recovered from 15 patients were characterized. The isolates, collected during January 2010 to December 2013, were retrospectively investigated by plasmid sequencing, molecular and fitness studies...
September 8, 2016: Journal of Antimicrobial Chemotherapy
Nicole Stoesser, Anna E Sheppard, Gisele Peirano, Robert Sebra, Tarah Lynch, Luke Anson, Andrew Kasarskis, Mary R Motyl, Derrick W Crook, Johann D Pitout
OXA-48-like enzymes have emerged as important extended-spectrum β-lactamases/carbapenemases in E. coli ST131. We report the structure of the first fully sequenced blaOXA-163 plasmid, and of two other blaOXA-48 plasmids in this lineage. blaOXA-163 was located on a 71kb IncN plasmid with other resistance genes. blaOXA-48 was present on IncL/M plasmids, genetically similar to other blaOXA-48 plasmid sequences, and consistent with inter-species/inter-lineage spread. The presence of blaOXA-48-like genes on epidemic plasmids in ST131 is of concern...
September 6, 2016: Antimicrobial Agents and Chemotherapy
Christa Ewers, Stephan Göttig, Maria Bülte, Sophie Fiedler, Manuela Tietgen, Ursula Leidner, Carsten Heydel, Rolf Bauerfeind, Torsten Semmler
Sequence type 131 (ST131) is one of the predominant Escherichia coli lineages among extraintestinal pathogenic E. coli (ExPEC) that causes a variety of diseases in humans and animals and frequently shows multidrug resistance. Here, we report the first genome sequence of an ST131-ExPEC strain from poultry carrying the plasmid-encoded colistin resistance gene mcr-1.
2016: Genome Announcements
Ágnes Sonnevend, Akela Ghazawi, Manaf Alqahtani, Atef Shibl, Wafa Jamal, Rayhan Hashmey, Tibor Pal
OBJECTIVES: Searching for the presence of the mcr-1 gene in colistin resistant Enterobacteriaceae in countries of the Arabian Peninsula. METHODS: Seventy-five independent, colistin resistant Enterobacteriaceae strains isolated from clinical cases in Bahrain, Kuwait, Oman, Saudi Arabia and the United Arab Emirates were tested by PCR for the mcr-1 gene. mcr-1 positive strains were genotyped, and their antibiotic susceptibility was established. The mcr-1 containing plasmids were mobilized into Escherichia coli K-12 and their sequence was determined...
September 2016: International Journal of Infectious Diseases: IJID
Agnė Giedraitienė, Astra Vitkauskienė, Alvydas Pavilonis, Vaiva Patamsytė, Nathalie Genel, Dominique Decre, Guillaume Arlet
BACKGROUND: Dissemination of multidrug-resistant Escherichia coli is closely associated with the worldwide spread of a single clone ST131, which is the main cause of urinary tract and bloodstream infections in patients from nursing homes and immunocompromised patients. The aim of our study was to determine the prevalence of ST131 clone and the replicons involved in the spread of blaCTX-M genes among O25b-ST131 CTX-M-producing E. coli isolates in Lithuania. METHODS: The strains included in this study were screened for CTX-M β-lactamase-encoding genes, phylogenetic groups and ST131 clone by PCR...
August 26, 2016: Infectious Diseases
Laura Fernandes Gonçalves, Paulo de Oliveira Martins-Júnior, Ana Beatriz Fabrício de Melo, Rafaella Christina Rocha Moreira da Silva, Vicente de Paulo Martins, André Pitondo-Silva, Tatiana Amabile de Campos
The aim of this work was to analyse extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains isolated from outpatients with signs of cystitis in Hospital Universitário de Brasília (Brasília, Brazil) during the period July 2013 to April 2014. E. coli isolated from urine culture were identified and their antibiotic susceptibility profile was determined by VITEK 2. ESBL-producing strains identified were submitted to PCR for Clermont phylotyping, CTX-M group typing and virulence determinant detection, and clonal relationships were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR...
September 2016: Journal of Global Antimicrobial Resistance
Rachel Park, Thomas F O'Brien, Susan S Huang, Meghan A Baker, Deborah S Yokoe, Martin Kulldorff, Craig Barrett, Jamie Swift, John Stelling
BACKGROUND: While antimicrobial resistance threatens the prevention, treatment, and control of infectious diseases, systematic analysis of routine microbiology laboratory test results worldwide can alert new threats and promote timely response. This study explores statistical algorithms for recognizing geographic clustering of multi-resistant microbes within a healthcare network and monitoring the dissemination of new strains over time. METHODS: Escherichia coli antimicrobial susceptibility data from a three-year period stored in WHONET were analyzed across ten facilities in a healthcare network utilizing SaTScan's spatial multinomial model with two models for defining geographic proximity...
November 2016: Expert Review of Anti-infective Therapy
Juraj Bosák, Lenka Micenková, Magda Doležalová, David Šmajs
Interactions of colicins U and Y with the OmpA (Outer membrane protein A) receptor molecule were studied using site-directed mutagenesis and colicin binding assay. A systematic mutagenesis of the colicin-susceptible OmpA sequence from Escherichia coli (OmpAEC) to the colicin-resistant OmpA sequence from Serratia marcescens (OmpASM) was performed in regions corresponding to extracellular OmpA loops 1-4. Susceptibility to colicins U and Y was significantly affected by the OmpA mutation in loop 1. As with functional analysis, a decrease in binding capacity of His-tagged colicin U was found for recombinant OmpA with a mutated segment in loop 1 compared to control OmpAEC...
August 2, 2016: International Journal of Medical Microbiology: IJMM
Irene Merino, Evelyn Shaw, Juan Pablo Horcajada, Emilia Cercenado, Beatriz Mirelis, M Angeles Pallarés, Juliá Gómez, M Xercavins, Luis Martínez-Martínez, Marina De Cueto, Rafael Cantón, Patricia Ruiz-Garbajosa
OBJECTIVES: The objective of this study was to assess the prevalence and molecular epidemiology of ESBL-producing Escherichia coli causing healthcare-associated (HCA) and community-associated (CA) bacteraemia of urinary origin (BUO) in Spain. METHODS: An observational cohort study was conducted at eight hospitals from different Spanish geographical areas (2010-11). BUO episodes (n = 425) were classified as HCA (n = 215) and CA (n = 210), and one blood isolate per episode was collected...
August 2016: Journal of Antimicrobial Chemotherapy
Xiaoqiang Liu, Kamoltip Thungrat, Dawn M Boothe
OBJECTIVE: The aim of this study was to explore the occurrence and molecular characterization of extended-spectrum β-lactamases (ESBL), plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemases among ESBL-producing multidrug resistant (MDR) Escherichia coli from dogs and cats in the United States. METHODS: Of 2443 E.coli isolated from dogs and cats collected between August 2009 and January 2013, 68 isolates were confirmed as ESBL-producing MDR ones. PCR and sequencing were performed to identify β-lactamases and plasmid-mediated quinolone resistance (PMQR) genes, and shed light on the virulence gene profiles, phylogenetic groups and ST types...
2016: Frontiers in Microbiology
Fusun Can, Özlem Kurt-Azap, Pelin İspir, Elif Nurtop, Ceren Seref, İlayda Loçlar, Ozge Nur Aktaş, Yelda Ceren Orhan, Onder Ergonul
In this study, risk factors for ST131 H30 and H30-Rx subclones among urinary tract infections (UTIs) caused by multidrug-resistant (MDR) Escherichia coli were described. Urine samples were collected from consecutive outpatients registered to the outpatient clinics of Başkent University Hospital (Ankara, Turkey) with complaints of acute cystitis in 2011. A total of 107 MDR E. coli isolates were included in the study. Of the 107 isolates studied, 26 (24.3%) were typed as ST131 clone. Extended-spectrum β-lactamase (ESBL)-producers accounted for 59 (55...
March 2016: Journal of Global Antimicrobial Resistance
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