keyword
MENU ▼
Read by QxMD icon Read
search

St131

keyword
https://www.readbyqxmd.com/read/28811343/comparative-genomics-of-escherichia-coli-isolated-from-skin-and-soft-tissue-and-other-extraintestinal-infections
#1
Amit Ranjan, Sabiha Shaik, Nishant Nandanwar, Arif Hussain, Sumeet K Tiwari, Torsten Semmler, Savita Jadhav, Lothar H Wieler, Munirul Alam, Rita R Colwell, Niyaz Ahmed
Escherichia coli, an intestinal Gram-negative bacterium, has been shown to be associated with a variety of diseases in addition to intestinal infections, such as urinary tract infections (UTIs), meningitis in neonates, septicemia, skin and soft tissue infections (SSTIs), and colisepticemia. Thus, for nonintestinal infections, it is categorized as extraintestinal pathogenic E. coli (ExPEC). It is also an opportunistic pathogen, causing cross infections, notably as an agent of zoonotic diseases. However, comparative genomic data providing functional and genetic coordinates for ExPEC strains associated with these different types of infections have not proven conclusive...
August 15, 2017: MBio
https://www.readbyqxmd.com/read/28801381/fluoroquinolone-resistance-mechanisms-by-molecular-epidemiologic-study-of-ciprofloxacin-nonsusceptible-escherichia-coli-sequence-types-isolated-from-clinical-specimens-in-a-tertiary-care-university-hospital-in-korea-emergence-of-clone-st131-2006-2008
#2
Hyung-Seok Yang, Youngjin Kim, Sun Young Cho, You Sun Nam, Tae Sang Oh, Kyung-Sun Park, Hyun-Chul Kim, Hee Joo Lee
To investigate the sequence types (STs) and fluoroquinolone resistance related mutations among ciprofloxacin (CIP)-non-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from Korean patients from 2006-2008. The prevalence of fluoroquinolone resistance-determining region (QRDR) mutations in gyrA, gyrB, parC, and parE and plasmid-mediated quinolone resistance (PMQR) genes were also studied. Multilocus sequence typing (MLST) was performed to identify STs. The most common ST was ST131 (33/51, 64...
August 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28797324/novel-mcr-3-variant-encoding-mobile-colistin-resistance-in-an-st131-escherichia-coli-isolate-from-bloodstream-infection-denmark-2014
#3
Louise Roer, Frank Hansen, Marc Stegger, Ute Wolff Sönksen, Henrik Hasman, Anette M Hammerum
A novel variant of the plasmid-borne colistin resistance gene mcr-3 was detected on an IncHI2 plasmid in an ST131 CTX-M-55-producing Escherichia coli isolate from a Danish patient with bloodstream infection in 2014. The discovery of novel plasmid-borne genes conferring resistance to colistin is of special interest since colistin has reemerged as an important drug in the treatment of infections with multidrug-resistant Gram-negative bacteria.
August 3, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28774984/complete-genome-sequence-of-blactx-m-27-encoding-escherichia-coli-strain-h105-of-sequence-type-131-lineage-c1-h30r
#4
Hiren Ghosh, Boyke Bunk, Swapnil Doijad, Judith Schmiedel, Linda Falgenhauer, Cathrin Spröer, Can Imirzalioglu, Jörg Overmann, Trinad Chakraborty
Escherichia coli sequence type 131 (ST131) is the most frequent antimicrobial-resistant lineage of E. coli, propagating extended-spectrum β-lactamases (ESBL) worldwide. Recently, an alarming rate of increase in isolates of the sublineage C1/H30R-blaCTX-M-27 of ST131 in geographically distant countries was reported. Here, we present the complete genome sequence of the ST131 sublineage C1/H30R E. coli isolate harboring blaCTX-M-27 from Germany.
August 3, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28738847/longitudinal-genomic-surveillance-of-multidrug-resistant-escherichia-coli-carriage-in-a-long-term-care-facility-in-the-united-kingdom
#5
Hayley J Brodrick, Kathy E Raven, Teemu Kallonen, Dorota Jamrozy, Beth Blane, Nicholas M Brown, Veronique Martin, M Estée Török, Julian Parkhill, Sharon J Peacock
BACKGROUND: Residents of long-term care facilities (LTCF) may have high carriage rates of multidrug-resistant pathogens, but are not currently included in surveillance programmes for antimicrobial resistance or healthcare-associated infections. Here, we describe the value derived from a longitudinal epidemiological and genomic surveillance study of drug-resistant Escherichia coli in a LTCF in the United Kingdom (UK). METHODS: Forty-five of 90 (50%) residents were recruited and followed for six months in 2014...
July 25, 2017: Genome Medicine
https://www.readbyqxmd.com/read/28720578/systematic-longitudinal-survey-of-invasive-escherichia-coli-in-england-demonstrates-a-stable-population-structure-only-transiently-disturbed-by-the-emergence-of-st131
#6
Teemu Kallonen, Hayley J Brodrick, Simon R Harris, Jukka Corander, Nicholas M Brown, Veronique Martin, Sharon J Peacock, Julian Parkhill
Escherichia coli associated with urinary tract infections and bacteremia has been intensively investigated, including recent work focusing on the virulent, globally disseminated, multidrug-resistant lineage ST131. To contextualize ST131 within the broader E. coli population associated with disease, we used genomics to analyze a systematic 11-yr hospital-based survey of E. coli associated with bacteremia using isolates collected from across England by the British Society for Antimicrobial Chemotherapy and from the Cambridge University Hospitals NHS Foundation Trust...
July 18, 2017: Genome Research
https://www.readbyqxmd.com/read/28709901/multicenter-collaboration-study-on-the-%C3%AE-lactam-resistant-enterobacteriaceae-in-japan-the-65th-anniversary-public-interest-purpose-project-of-the-japanese-society-of-chemotherapy
#7
Yoshikazu Ishii, Kotaro Aoki, Kazuhiro Tateda, Hiroshi Kiyota
This study was conducted using the same method of the "1-day multicenter collaborative research for Clostridium difficile infectious". This surveillance study was performed by 38 participating medical facilities across Japan. Of the 268 fecal specimens collected in this study, 5 (1.9%) carbapenem-resistant Enterobacteriaceae (CRE) were judged carbapenem resistant by the criteria of the Infectious Disease Act, Japan (2 mg/L or more and 64 mg/L or more MICs for imipenem and cefmetazole, respectively). However, no isolate was shown to have a meropenem MIC of 2 mg/L or more...
September 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28700648/highly-diverse-and-antimicrobial-susceptible-escherichia-coli-display-a-na%C3%A3-ve-bacterial-population-in-fruit-bats-from-the-republic-of-congo
#8
Kathrin Nowak, Jakob Fahr, Natalie Weber, Antina Lübke-Becker, Torsten Semmler, Sabrina Weiss, Jean-Vivien Mombouli, Lothar H Wieler, Sebastian Guenther, Fabian H Leendertz, Christa Ewers
Bats are suspected to be a reservoir of several bacterial and viral pathogens relevant to animal and human health, but studies on Escherichia coli in these animals are sparse. We investigated the presence of E. coli in tissue samples (liver, lung and intestines) collected from 50 fruit bats of five different species (Eidolon helvum, Epomops franqueti, Hypsignathus monstrosus, Myonycteris torquata, Rousettus aegyptiacus) of two different areas in the Republic of Congo between 2009 and 2010. To assess E. coli pathotypes and phylogenetic relationships, we determined the presence of 59 virulence associated genes and multilocus sequence types (STs)...
2017: PloS One
https://www.readbyqxmd.com/read/28686503/characterization-of-carbapenem-resistant-escherichia-coli-isolates-through-the-whole-genome-sequencing-analysis
#9
Yanping Luo, Rong Luo, Hong Ding, Xiu Ren, Haipeng Luo, Ying Zhang, Liyan Ye, Shenghui Cui
Limited studies have characterized multiple carbapenem-resistant Escherichia coli isolates and compared their genetic characteristics through whole-genome sequencing (WGS) because of the scarcity of these isolates. In this study, we determined the prevalence of carbapenem resistance in 2,993 E. coli isolates collected from three Chinese hospitals. The carbapenem-resistant isolates were further characterized by WGS, and the molecular epidemiological characters and resistance mechanisms were explored through the publicly available platforms from the Center for Genomic Epidemiology...
July 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28684862/direct-and-convenient-measurement-of-plasmid-stability-in-lab-and-clinical-isolates-of-e-coli
#10
Siyi Chen, Mårten Larsson, Robert C Robinson, Swaine L Chen
Plasmids are important mobile elements in bacteria, contributing to evolution, virulence, and antibiotic resistance. Natural plasmids are generally large and maintained at low copy number and thus prone to be lost. Therefore, dedicated plasmid maintenance systems have evolved, leading to plasmid loss rates as low as 1 per 10(7) divisions. These low rates complicate studies of plasmid loss, as traditional techniques for measuring plasmid loss are laborious and not quantitative. To overcome these limitations, we leveraged a stringent negative selection system to develop a method for performing direct, quantitative measurements of plasmid loss in E...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28655557/rapid-identification-of-st131-escherichia-coli-by-a-novel-multiplex-real-time-allelic-discrimination-assay
#11
Patrice François, Eve-Julie Bonetti, Carolina Fankhauser, Damien Baud, Abdessalam Cherkaoui, Jacques Schrenzel, Stephan Harbarth
Escherichia coli sequence type 131 is increasingly described in severe hospital infections. We developed a rapid real-time allelic discrimination assay for the rapid identification of E. coli ST131 isolates. This rapid assay represents an affordable alternative to sequence-based strategies before completing characterization of potentially highly virulent isolates of E. coli.
June 26, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28638846/the-pandemic-h30-subclone-of-sequence-type-131-st131-as-the-leading-cause-of-multidrug-resistant-escherichia-coli-infections-in-the-united-states-2011-2012
#12
James R Johnson, Stephen Porter, Paul Thuras, Mariana Castanheira
BACKGROUND: Extraintestinal Escherichia coli infections are increasingly challenging due to emerging antimicrobial resistance, including resistance to extended-spectrum beta-lactams and fluoroquinolones. Sequence type 131 (ST131) is a leading contributor. METHODS: Three hundred sixty E. coli clinical isolates from across the United States (2011-2012), selected randomly from the SENTRY collection within 3 resistance categories (extended-spectrum cephalosporin [ECS]-reduced susceptibility [RS]; fluoroquinolone-resistant, ESC-susceptible; and fluoroquinolone-susceptible, ESC-susceptible) were typed for phylogroup, sequence type complex (STc), subsets thereof, virulence genotype, O type, and beta-lactamase genes...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28630184/occurrence-of-clinically-important-lineages-including-the-st131-c1-m27-subclone-among-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-wastewater
#13
Ryota Gomi, Tomonari Matsuda, Yasufumi Matsumura, Masaki Yamamoto, Michio Tanaka, Satoshi Ichiyama, Minoru Yoneda
Contamination of environmental waters by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBLEC) is of great concern. Wastewater treatment plants (WWTPs) and hospitals release large amounts of ESBLEC into the environment. In the present study, we isolated ESBLEC strains from wastewater collected from a WWTP and a hospital in Japan and performed whole-genome sequencing to characterize these strains. Genomic analysis of 54 strains (32 from the WWTP and 22 from hospital wastewater) revealed the occurrence of clinically important clonal groups with extraintestinal pathogenic E...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28592545/development-of-a-web-tool-for-escherichia-coli-subtyping-based-on-fimh-alleles
#14
Louise Roer, Veronika Tchesnokova, Rosa Allesøe, Mariya Muradova, Sujay Chattopadhyay, Johanne Ahrenfeldt, Martin C F Thomsen, Ole Lund, Frank Hansen, Anette M Hammerum, Evgeni Sokurenko, Henrik Hasman
The aim of this study was to construct a valid publicly available method for in silico fimH subtyping of Escherichia coli particularly suitable for differentiation of fine-resolution subgroups within clonal groups defined by standard multilocus sequence typing (MLST). FimTyper was constructed as a FASTA database containing all currently known fimH alleles. The software source code is publicly available at https://bitbucket.org/genomicepidemiology/fimtyper, the database is freely available at https://bitbucket...
August 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28584160/rapid-identification-of-different-escherichia-coli-sequence-type-131-clades
#15
Yasufumi Matsumura, Johann D D Pitout, Gisele Peirano, Rebekah DeVinney, Taro Noguchi, Masaki Yamamoto, Ryota Gomi, Tomonari Matsuda, Satoshi Nakano, Miki Nagao, Michio Tanaka, Satoshi Ichiyama
Escherichia coli sequence type 131 (ST131) is a pandemic clonal lineage that is responsible for the global increase in fluoroquinolone resistance and extended-spectrum-β-lactamase (ESBL) producers. The members of ST131 clade C, especially subclades C2 and C1-M27, are associated with ESBLs. We developed a multiplex conventional PCR assay with the ability to detect all ST131 clades (A, B, and C), as well as C subclades (C1-M27, C1-nM27 [C1-non-M27], and C2). To validate the assay, we used 80 ST131 global isolates that had been fully sequenced...
August 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28584139/genomic-analysis-of-multidrug-resistant-escherichia-coli-from-north-carolina-community-hospitals-ongoing-circulation-of-ctx-m-producing-st131-h30rx-and-st131-h30r1-strains
#16
Hajime Kanamori, Christian M Parobek, Jonathan J Juliano, James R Johnson, Brian D Johnston, Timothy J Johnson, David J Weber, William A Rutala, Deverick J Anderson
Escherichia coli sequence type 131 (ST131) predominates globally among multidrug-resistant (MDR) E. coli strains. We used whole-genome sequencing (WGS) to investigate 63 MDR E. coli isolates from 7 North Carolina community hospitals (2010 to 2015). Of these, 39 (62%) represented ST131, including 37 (95%) from the ST131-H30R subclone: 10 (27%) from its H30R1 subset and 27 (69%) from its H30Rx subset. ST131 core genomes differed by a median of 15 (range, 0 to 490) single-nucleotide variants (SNVs) overall versus only 7 within H30R1 (range, 3 to 12 SNVs) and 11 within H30Rx (range, 0 to 21)...
August 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28552831/extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-healthy-humans-in-mexico-including-subclone-st131-b2-o25-h4-h30-rx
#17
Gerardo Cortés-Cortés, Patricia Lozano-Zarain, Carmen Torres, Carla Andrea Alonso, Ana María Ríos-Torres, Miguel Castañeda, Liliana López-Pliego, Armando Navarro, Rosa Del Carmen Rocha-Gracia
OBJECTIVES: The resistance mechanisms, molecular type and plasmid content of cefotaxime-resistant Escherichia coli isolated from faecal samples of healthy volunteers in Puebla, Mexico, were characterised. METHODS AND RESULTS: Cefotaxime-resistant E. coli were recovered from 11 (18%) of 60 healthy volunteers. The isolates (one per sample) were characterised as multidrug-resistant and phenotypically extended-spectrum β-lactamase (ESBL)-producing strains. Genes encoding resistance to β-lactams (blaCTX-M-15, blaCTX-M-14a, blaCTX-M-14b, blaOXA-1, blaTEM-1b), quinolones [aac(6')-Ib-cr, qnrB19], aminoglycosides [aac(3')-II] and tetracycline [tet(A), tet(B)] were detected among the 11 ESBL-producing E...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28550370/molecular-epidemiology-of-escherichia-coli-sequence-type-131-and-its-h30-h30-rx-subclones-recovered-from-extra-intestinal-infections-first-report-of-oxa-48-producing-st131-clone-from-iran
#18
Z Hojabri, M Mirmohammadkhani, F Kamali, K Ghassemi, S Taghavipour, O Pajand
Multidrug-resistant (MDR) O25b-ST131 clone of Escherichia coli is well established as a significant cause of extra-intestinal infections worldwide. However, there have been no studies about the prevalence of ST131 and its H30/H30Rx subclones from Iran. The prevalence of ST131 was 29.8% among phylogroups B2, D, and F of E.coli isolates recovered from extra-intestinal infections. Fifty-seven (90.4%) and six (9.6%) of isolates belonged to serogroups O25b and O16 respectively, and exhibited high rates of MDR (98...
May 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28533233/epidemic-emergence-in-the-united-states-of-escherichia-coli-sequence-type-131-h30-st131-h30-2000-to-2009
#19
James R Johnson, Stephen Porter, Paul Thuras, Mariana Castanheira
The H30 subclone of Escherichia coli sequence type 131 (ST131-H30) has become the leading antimicrobial resistance E. coli lineage in the United States and often exhibits resistance to one or both of the two key antimicrobial classes for treating Gram-negative infections, extended-spectrum cephalosporins (ESCs) and fluoroquinolones (FQs). However, the timing of and reasons for its recent emergence are inadequately defined. Accordingly, from E. coli clinical isolates collected systematically across the United States by the SENTRY Antimicrobial Surveillance Program in 2000, 2003, 2006, and 2009, 234 isolates were selected randomly, stratified by year, within three resistance categories: (i) ESC-reduced susceptibility, regardless of FQ phenotype (ESC-RS); (ii) FQ resistance, ESC susceptible (FQ-R); and (iii) FQ susceptible, ESC susceptible (FQ-S)...
August 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28521000/the-ecology-of-extended-spectrum-%C3%AE-lactamases-esbls-in-the-developed-world
#20
Yohei Doi, Alina Iovleva, Robert A Bonomo
Background: Since the initial appearance in the 1980s, Enterobacteriaceae producing extended-spectrum β-lactamase (ESBL) have increased in prevalence and emerged as a major antimicrobial-resistant pathogen. The source of these antimicrobial-resistant bacteria in the developed world is an area of active investigation. Methods: A standard internet search was conducted with a focus on the epidemiology and potential sources of ESBL-producing Enterobacteriaceae in the developed world...
April 1, 2017: Journal of Travel Medicine
keyword
keyword
304
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"