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https://www.readbyqxmd.com/read/29324785/phylogenetic-diversity-antimicrobial-susceptibility-and-virulence-gene-profiles-of-brachyspira-hyodysenteriae-isolates-from-pigs-in-germany
#1
Jessica Joerling, Stefanie A Barth, Karen Schlez, Hermann Willems, Werner Herbst, Christa Ewers
Swine dysentery (SD) is an economically important diarrheal disease in pigs caused by different strongly hemolytic Brachyspira (B.) species, such as B. hyodysenteriae, B. suanatina and B. hampsonii. Possible associations of epidemiologic data, such as multilocus sequence types (STs) to virulence gene profiles and antimicrobial susceptibility are rather scarce, particularly for B. hyodysenteriae isolates from Germany. In this study, B. hyodysenteriae (n = 116) isolated from diarrheic pigs between 1990 and 2016 in Germany were investigated for their STs, susceptibility to the major drugs used for treatment of SD (tiamulin and valnemulin) and genes that were previously linked with virulence and encode for hemolysins (tlyA, tlyB, tlyC, hlyA, BHWA1_RS02885, BHWA1_RS09085, BHWA1_RS04705, and BHWA1_RS02195), outer membrane proteins (OMPs) (bhlp16, bhlp17...
2018: PloS One
https://www.readbyqxmd.com/read/29279444/detection-of-escherichia-coli-producing-ctx-m-1-group-extended-spectrum-%C3%AE-lactamases-from-pigs-in-aichi-prefecture-japan-between-2015-and-2016
#2
Chihiro Norizuki, Kumiko Kawamura, Jun-Ichi Wachino, Masahiro Suzuki, Noriyuki Nagano, Takaaki Kondo, Yoshichika Arakawa
We investigated the prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from Japanese pigs. A total of 345 pig fecal specimens were collected from 30 farms in Aichi prefecture, Japan, between June 2015 and April 2016, and 22 unique ESBL-producing E. coli were isolated from 16 samples of eight farms. The ESBL types included CTX-M-15 (54.5%), CTX-M-55 (27.2%), CTX-M-3 (0.9%), and CTX-M-14 (0.9%). The predominant plasmid replicon type was IncN, and they carried blaCTX-M-55...
December 26, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29258889/association-of-escherichia-coli-st131-lineage-with-risk-of-urinary-tract-infection-recurrence-among-young-women
#3
Miriam D Ismail, Ihsan Ali, Savannah Hatt, Elizabeth A Salzman, Anna W Cronenwett, Carl F Marrs, Alexander H Rickard, Betsy Foxman
OBJECTIVES: Test the hypothesis that urinary tract infections (UTI) caused by Escherichia coli of the ST131 lineage are more likely to recur than UTI cause by other E. coli lineages. METHODS: We used isolates from 221 young women with UTI caused by Escherichia coli participating in a randomized controlled trial. Participants were followed for 6 months or until recurrence. RESULTS: Sequence type was not associated with risk of recurrence. Isolates in the ST131 lineage were more resistant to quinolones (6% vs 1%) than other STs, but not trimethoprim sulfamethoxazole (15% vs 15%)...
December 16, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29253152/whole-genome-analysis-of-cephalosporin-resistant-escherichia-coli-from-bloodstream-infections-in-australia-new-zealand-and-singapore-high-prevalence-of-cmy-2-producers-and-st131-carrying-blactx-m-15-and-blactx-m-27
#4
Patrick N A Harris, Nouri L Ben Zakour, Leah W Roberts, Alexander M Wailan, Hosam M Zowawi, Paul A Tambyah, David C Lye, Roland Jureen, Tau H Lee, Mo Yin, Ezlyn Izharuddin, David Looke, Naomi Runnegar, Benjamin Rogers, Hasan Bhally, Amy Crowe, Mark A Schembri, Scott A Beatson, David L Paterson
Objectives: To characterize MDR Escherichia coli from bloodstream infections (BSIs) in Australia, New Zealand and Singapore. Methods: We collected third-generation cephalosporin-resistant (3GC-R) E. coli from blood cultures in patients enrolled in a randomized controlled trial from February 2014 to August 2015. WGS was used to characterize antibiotic resistance genes, MLST, plasmids and phylogenetic relationships. Antibiotic susceptibility was determined using disc diffusion and Etest...
December 14, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29250044/clonal-diversity-virulence-potential-and-antimicrobial-resistance-of-escherichia-coli-causing-community-acquired-urinary-tract-infection-in-switzerland
#5
Magdalena T Nüesch-Inderbinen, Melinda Baschera, Katrin Zurfluh, Herbert Hächler, Hansjakob Nüesch, Roger Stephan
Objectives: The aim of this study was to assess the clonal structure, virulence potential and antibiotic susceptibility of uropathogenic Escherichia coli (UPEC) isolates causing community acquired urinary tract infection (CAUTI) in unselected primary care patients in Switzerland. Methods: We performed multilocus sequence typing, virulence factor determination, and phenotypic and genotypic antimicrobial resistance testing on 44 non-duplicate UPEC isolates. Results: Twenty-seven different sequence types (STs) were identified...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29236574/genetic-diversity-of-kpc-producing-escherichia-coli-klebsiella-oxytoca-serratia-marcescens-and-citrobacter-freundii-isolates-from-argentina
#6
Denise De Belder, Celeste Lucero, Melina Rapoport, Adriana Rosato, Diego Faccone, Alejandro Petroni, Fernando Pasteran, Ezequiel Albornoz, Alejandra Corso, Sonia A Gomez
The predominance of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae was caused by the spread of ST258 clone. In Latin America, KPC was reported in 2006, with the isolation of genetically unrelated K. pneumoniae in Colombia. Since then, the expansion of blaKPC in either K. pneumoniae ST258 or other Enterobacteriaceae (ETB) species was increasingly reported. In this study, we characterized 89 KPC-producing Escherichia coli, Klebsiella oxytoca, Serratia marcescens, and Citrobacter freundii that were received between 2010 and 2014...
December 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29214016/genotypic-characterization-of-gentamicin-and-cephalosporin-resistant-escherichia-coli-isolates-from-blood-cultures-in-a-norwegian-university-hospital-2011-2015
#7
Øyvind Andreas Fladberg, Silje Bakken Jørgensen, Hege Vangstein Aamot
Background: Cephalosporin resistance in clinical E. coli isolates is increasing internationally. The increase has been caused by virulent and often multidrug-resistant clones, especially the extended spectrum β-lactamase (ESBL) producing E. coli clone O25b-ST131. Methods: In Norway, recommended empirical treatment of sepsis consists of gentamicin and penicillin combined, or a broad-spectrum cephalosporin. To investigate if increased gentamicin and cephalosporins resistance rates in our hospital could be caused by specific clones, we conducted a retrospective study on E...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29209300/molecular-characteristics-of-st1193-clone-among-phylogenetic-group-b2-non-st131-fluoroquinolone-resistant-escherichia-coli
#8
Jing Wu, Fangjun Lan, Yanfang Lu, Qingwen He, Bin Li
Objectives: Sequence type 1193 is emerging as a new, virulent and resistant lineage among fluoroquinolone resistant Escherichia coli (FQrE. coli). In this study, we investigated the prevalence and molecular characteristics of this clone isolated from a Chinese university hospital. Methods: 73 phylogenetic group B2-FQr-non-ST131 isolates were collected from August 2014 and August 2015 at a Chinese university hospital. Isolates were screened for ST1193 by multilocus sequence typing. E. coli ST1193 then underwent lactose fermentation determination, susceptibility testing, virulence genotyping, PCR-based O typing, pulsed-field gel electrophoresis (PFGE) and FQr mechanism analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29190804/virulence-genes-and-subclone-status-as-markers-of-experimental-virulence-in-a-murine-sepsis-model-among-escherichia-coli-sequence-type-131-clinical-isolates-from-spain
#9
Irene Merino, Stephen B Porter, Brian D Johnston, Connie Clabots, Evelyn Shaw, Juan Pablo Horcajada, Rafael Cantón, Patricia Ruiz-Garbajosa, James R Johnson
OBJECTIVE: To assess experimental virulence among sequence type 131 (ST131) Escherichia coli bloodstream isolates in relation to virulence genotype and subclone. METHODS: We analysed 48 Spanish ST131 bloodstream isolates (2010) by PCR for ST131 subclone status (H30Rx, H30 non-Rx, or non-H30), virulence genes (VGs), and O-type. Then we compared these traits with virulence in a murine sepsis model, as measured by illness severity score (ISS) and rapid lethality (mean ISS ≥ 4)...
2017: PloS One
https://www.readbyqxmd.com/read/29180984/risk-of-transmission-of-antimicrobial-resistant-escherichia-coli-from-commercial-broiler-and-free-range-retail-chicken-in-india
#10
Arif Hussain, Sabiha Shaik, Amit Ranjan, Nishant Nandanwar, Sumeet K Tiwari, Mohammad Majid, Ramani Baddam, Insaf A Qureshi, Torsten Semmler, Lothar H Wieler, Mohammad A Islam, Dipshikha Chakravortty, Niyaz Ahmed
Multidrug-resistant Escherichia coli infections are a growing public health concern. This study analyzed the possibility of contamination of commercial poultry meat (broiler and free-range) with pathogenic and or multi-resistant E. coli in retail chain poultry meat markets in India. We analyzed 168 E. coli isolates from broiler and free-range retail poultry (meat/ceca) sampled over a wide geographical area, for their antimicrobial sensitivity, phylogenetic groupings, virulence determinants, extended-spectrum-β-lactamase (ESBL) genotypes, fingerprinting by Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and genetic relatedness to human pathogenic E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29167743/prevalence-and-characteristics-of-st131-clone-among-unselected-clinical-escherichia-coli-in-a-chinese-university-hospital
#11
Bin Li, Yanfang Lu, Fangjun Lan, Qingwen He, Chen Li, Yingping Cao
Background: Escherichia coli clinical sequence type 131 (ST131) has emerged as an extensively antimicrobial resistant E. coli clonal group in recent years throughout the world. The aim of this study was to investigate the prevalence and molecular characteristics of ST131 among unselected E. coli clinical isolates in a Chinese university hospital. Methods: Seven hundred consecutive E. coli isolates were collected at a Chinese university hospital between 2014 and 2015...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29142104/constraints-on-lateral-gene-transfer-in-promoting-fimbrial-usher-protein-diversity-and-function
#12
Christopher J Stubenrauch, Gordon Dougan, Trevor Lithgow, Eva Heinz
Fimbriae are long, adhesive structures widespread throughout members of the family Enterobacteriaceae. They are multimeric extrusions, which are moved out of the bacterial cell through an integral outer membrane protein called usher. The complex folding mechanics of the usher protein were recently revealed to be catalysed by the membrane-embedded translocation and assembly module (TAM). Here, we examine the diversity of usher proteins across a wide range of extraintestinal (ExPEC) and enteropathogenic (EPEC) Escherichia coli, and further focus on a so far undescribed chaperone-usher system, with this usher referred to as UshC...
November 2017: Open Biology
https://www.readbyqxmd.com/read/29136156/increase-in-antimicrobial-resistance-and-emergence-of-major-international-high-risk-clonal-lineages-in-dogs-and-cats-with-urinary-tract-infection-16-year-retrospective-study
#13
Cátia Marques, Adriana Belas, Andreia Franco, Catarina Aboim, Luís Telo Gama, Constança Pomba
Objectives: To evaluate temporal trends in antimicrobial resistance, over 16 years, in bacteria isolated from dogs and cats with urinary tract infection (UTI) and the clonal lineages of bacteria harbouring critical antimicrobial resistance mechanisms. Methods: Antimicrobial susceptibility testing was conducted for 948 bacteria isolated from dogs and cats with UTI (1999-2014). Resistance mechanisms were detected by PCR, namely ESBL/AmpC in third-generation cephalosporin (3GC)-resistant Escherichia coli and Proteus mirabilis, mecA in methicillin-resistant staphylococci, and aac(6')-Ieaph(2″)-Ia and aph(2″)-1d in high-level gentamicin-resistant (HLGR) enterococci...
November 9, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29116143/risk-factors-and-molecular-features-of-sequence-type-st-131-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-community-onset-bacteremia
#14
Hyunsoo Kim, Young Ah Kim, Yoon Soo Park, Min Hyuk Choi, Gyeong In Lee, Kyungwon Lee
Due to the spread of a single CTX-M-type extended-spectrum β-lactamase (ESBL) clone of sequence type (ST) 131, community-onset bacteremia caused by ESBL-producing Escherichia coli has increased dramatically. We evaluated the risk factors and molecular features of ESBL-producing E. coli ST131 clones isolated from Korean patients with community-onset bacteremia. We collected a total of 124 ESBL-producing E. coli isolates from blood in patients with community-onset bacteremia over a 2 year-period. Among 124 patients, the number of community-associated bacteremia cases was 57 (46%)...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29102775/blandm-1-producing-multidrug-resistant-escherichia-coli-isolated-from-a-companion-dog-in-china
#15
Lanqing Cui, Lei Lei, Yuan Lv, Rongmin Zhang, Xiaoyu Liu, Mei Li, Fan Zhang, Yang Wang
OBJECTIVES: This sudy aimed to characterize a blaNDM-1-producing Escherichia coli from a companion dog. METHODS: This strain was isolated from a surveillance study of carbapenem-resistant Gram-negative bacteria and the specie was identified by API 20E system and MALDI-TOF MS. MICs of various antimicrobial agents were determined by agar dilution. Multilocus sequence typing (MLST) and various carbapenemase genes including blaNDM, blaIMP, blaVIM, blaSME, blaIMI, blaKPC, blaGES, blaSPM, blaGIM, blaNMC, blaDIM, blaSIM, blaOXA-23, blaOXA-24, blaOXA-48, blaOXA-51 and blaOXA-58 were screened by PCR...
November 1, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29102123/convergence-of-plasmid-architectures-drives-emergence-of-multi-drug-resistance-in-a-clonally-diverse-escherichia-coli-population-from-a-veterinary-clinical-care-setting
#16
Sam Wagner, Nadejda Lupolova, David L Gally, Sally A Argyle
The purpose of this study was to determine the plasmid architecture and context of resistance genes in multi-drug resistant (MDR) Escherichia coli strains isolated from urinary tract infections in dogs. Illumina and single-molecule real-time (SMRT) sequencing were applied to assemble the complete genomes of E. coli strains associated with clinical urinary tract infections, which were either phenotypically MDR or drug susceptible. This revealed that multiple distinct families of plasmids were associated with building an MDR phenotype...
November 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29091192/modifications-in-the-pmrb-gene-are-the-primary-mechanism-for-the-development-of-chromosomally-encoded-resistance-to-polymyxins-in-uropathogenic-escherichia-coli
#17
Minh-Duy Phan, Nguyen Thi Khanh Nhu, Maud E S Achard, Brian M Forde, Kar Wai Hong, Teik Min Chong, Wai-Fong Yin, Kok-Gan Chan, Nicholas P West, Mark J Walker, David L Paterson, Scott A Beatson, Mark A Schembri
Objectives: Polymyxins remain one of the last-resort drugs to treat infections caused by MDR Gram-negative pathogens. Here, we determined the mechanisms by which chromosomally encoded resistance to colistin and polymyxin B can arise in the MDR uropathogenic Escherichia coli ST131 reference strain EC958. Methods: Two complementary approaches, saturated transposon mutagenesis and spontaneous mutation induction with high concentrations of colistin and polymyxin B, were employed to select for mutations associated with resistance to polymyxins...
October 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29066550/comparative-genomic-analysis-of-globally-dominant-st131-clone-with-other-epidemiologically-successful-extraintestinal-pathogenic-escherichia-coli-expec-lineages
#18
Sabiha Shaik, Amit Ranjan, Sumeet K Tiwari, Arif Hussain, Nishant Nandanwar, Narender Kumar, Savita Jadhav, Torsten Semmler, Ramani Baddam, Mohammed Aminul Islam, Munirul Alam, Lothar H Wieler, Haruo Watanabe, Niyaz Ahmed
Escherichia coli sequence type 131 (ST131), a pandemic clone responsible for the high incidence of extraintestinal pathogenic E. coli (ExPEC) infections, has been known widely for its contribution to the worldwide dissemination of multidrug resistance. Although other ExPEC-associated and extended-spectrum-β-lactamase (ESBL)-producing E. coli clones, such as ST38, ST405, and ST648 have been studied widely, no comparative genomic data with respect to other genotypes exist for ST131. In this study, comparative genomic analysis was performed for 99 ST131 E...
October 24, 2017: MBio
https://www.readbyqxmd.com/read/29061749/heteroresistance-to-piperacillin-tazobactam-in-clinical-isolates-of-escherichia-coli-st131
#19
Ángel Rodríguez-Villodres, José Manuel Ortiz de la Rosa, Rocío Álvarez-Marín, Jerónimo Pachón, Javier Aznar, José Antonio Lepe, Younes Smani
Heteroresistance to piperacillin/tazobactam has only been reported in Pseudomonas aeruginosa (1).….
October 23, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29040337/a-comparison-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-from-clinical-recreational-water-and-wastewater-samples-associated-in-time-and-location
#20
COMPARATIVE STUDY
Silje B Jørgensen, Arne V Søraas, Lotte S Arnesen, Truls M Leegaard, Arnfinn Sundsfjord, Pål A Jenum
Extended spectrum β-lactamase producing Escherichia coli (ESBL-EC) are excreted via effluents and sewage into the environment where they can re-contaminate humans and animals. The aim of this observational study was to detect and quantify ESBL-EC in recreational water and wastewater, and perform a genetic and phenotypic comparative analysis of the environmental strains with geographically associated human urinary ESBL-EC. Recreational fresh- and saltwater samples from four different beaches and wastewater samples from a nearby sewage plant were filtered and cultured on differential and ESBL-selective media...
2017: PloS One
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