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Female infertility

Ahmed M Eissa, Flavio S V Barros, Pavle Vrljicak, Jan J Brosens, Neil R Cameron
Novel approaches for culturing primary human cells in vitro are increasingly needed to study cell and tissue physiology and to grow replacement tissue for regenerative medicine. Conventional 2D monolayer cultures of endometrial epithelial and stromal cells fail to replicate the complex 3D architecture of tissue. A fully synthetic scaffold that mimics the microenvironment of the human endometrium can ultimately provide a robust platform for investigating tissue physiology and, hence, take significant steps towards tackling female infertility and IVF failure...
June 21, 2018: Biomacromolecules
Rocco Malivindi, Marta Santoro, Daniela De Rose, Salvatore Panza, Serena Gervasi, Vittoria Rago, Saveria Aquila
The farnesoid X receptor alpha (FXR) is a bile acid sensor activated by binding to endogenous bile acids including chenodeoxycholic acid (CDCA). Although, FXR is expressed in male reproductive tissue, the relevance of the receptor on reproduction is scarcely known. Here we demonstrated the FXR presence and its action on several human sperm features. Western blot and Immunofluorescence assays evidenced the FXR expression in human spermatozoa and the localization in the middle piece. CDCA increasing concentrations and GW4064, synthetic ligand of FXR, were used to study the FXR influence on sperm motility, survival, capacitation, acrosome reaction and on glucose as well as lipid metabolism...
June 19, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Momoko Kobayashi, Kaya Watanabe, Ritsuko Matsumura, Nozomi Anayama, Akio Miyamoto, Hitoshi Miyazaki, Koyomi Miyazaki, Takashi Shimizu, Makoto Akashi
Circadian dysfunction perturbs the female reproductive cycle. In particular, mice lacking the clock gene Bmal1 show severe infertility, implying that BMAL1 plays roles in ovulation and luteinization. Here, we examined temporal changes in clock gene expression in the ovary and oviduct before and during gonadotropin-induced follicular growth, ovulation, and luteinization in sexually immature mice. While the oviduct did not show a drastic change in clock gene expression, Bmal1 expression in the ovary was higher than that in control mice during the period from 4 to 16 hr after human chorionic gonadotropin (hCG) administration...
June 19, 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Reza Omani-Samani, Azadeh Ghaheri, Behnaz Navid, Mahdi Sepidarkish, Saman Maroufizadeh
BACKGROUND: Generalized anxiety disorder (GAD) is one of the most prevalent anxiety disorders among infertile patients. This study aimed to determine the prevalence of GAD and its associated factors among infertile patients in Tehran, Iran. METHODS: This cross-sectional study included 1146 infertile patients in a referral fertility center in Tehran, Iran between May and October 2017. GAD was measured using the Generalized Anxiety Disorder-7 (GAD-7) scale. The associations between GAD and demographic/fertility characteristics were estimated using simple and multiple logistic regression with odds ratio (OR) and 95% confidence interval (CI)...
June 19, 2018: Health and Quality of Life Outcomes
C Chen, X Xu, L Kong, P Li, F Zhou, S Zhao, X Xin, J Tan, X Zhang
Empty follicle syndrome (EFS) is a disorder associated with female infertility and presents as a complete failure to retrieve oocytes during ART cycles despite normal follicle development and careful aspiration. To date, only two EFS cases have been reported with homozygous missense mutations in the luteinizing hormone/chorionic gonadotropin receptor (LHCGR) gene, and both cases showed normal estradiol (E2) production during ovulation induction. The molecular genetic mechanisms of EFS remain unknown. Herein, we report two novel homozygous inactivating LHCGR mutations, c...
June 15, 2018: Human Reproduction
Junyan Sun, Ying Guo, Qiuwan Zhang, Shixia Bu, Boning Li, Qian Wang, Dongmei Lai
Psychological stress, which exerts detrimental effects on human reproduction, may compromise the meiotic competence of oocytes. Meiotic resumption, germinal vesicle breakdown (GVBD), is the first milestone to confer meiotic competence to oocytes. In the practice of assisted reproductive technology (ART), the timing for GVBD is associated with the rates of cleavage and blastocyst formation. However, whether chronic stress compromises oocyte competence by influencing GVBD and the underlying mechanisms are unclear...
June 18, 2018: Cell Cycle
Sabina Zacharieva, Ralitsa Robeva, Silvia Andonova, Radoslava Vazharova, Lubomir Balabanski, Maya Atanasoska, Draga Toncheva, Atanaska Elenkova, Alexey Savov
11β-Hydroxylase deficiency is the second most common enzyme disorder after 21-hydroxylase deficiency causing congenital adrenal hyperplasia (CAH11β ). In females, the clinical phenotype of CAH11β classic forms is associated with ambiguous genitalia, virilization and hypertension, while most common complaints in milder non-classic forms include hirsutism, acne, menstrual disturbances, and infertility. Herein, we present clinical and genetic characteristics of an adult woman with 11β-hydroxylase deficiency, hypertension and infertility; she has been followed up from her first pregnancy to her early menopause...
June 17, 2018: Gynecological Endocrinology
Asmaa Ali, Bhavesh V Mistry, Hala A Ahmed, Razan Abdulla, Hassan A Amer, Abdelbary Prince, Anas M Alazami, Fowzan S Alkuraya, Abdullah Assiri
DDB1- and CUL4-associated factor 17 (Dcaf17) is a member of DCAF family genes that encode substrate receptor proteins for Cullin-RING E3 ubiquitin ligases, which play critical roles in many cellular processes. To unravel the function of DCAF17, we performed expression profiling of Dcaf17 in different tissues of wild type mouse by qRT-PCR and generated Dcaf17 knockout mice by gene targeting. Expression profiling of Dcaf17 showed highest expression in testis. Analyses of Dcaf17 transcripts during post-natal development of testis at different ages displayed gradual increase in Dcaf17 mRNA levels with the age...
June 15, 2018: Scientific Reports
Xiaotong Wang, Haixiong Lin, Mingzhu Chen, Jian Wang, Yuanlin Jin
BACKGROUND: Although many patients try to seek acupuncture to improve in vitro fertilization (IVF) outcomes, evidence regarding its efficacy and acupoints characters are lacking. The aim of this protocol is to evaluate the effectiveness and safety, as well as the acupoints characteristics of acupuncture in the treatment of female undergoing IVF, by conducting a systematic review and data mining. METHODS: The following 6 databases will be searched from their inception to April 30, 2018: PubMed, Chinese National Knowledge Infrastructure, Wanfang, VIP database, Embase, and Cochrane Library...
June 2018: Medicine (Baltimore)
Yiping Wang, Xu Xiong, Lydia Bazzano, Emily W Harville
BACKGROUND: Although childhood cardiovascular risk can contribute to adult cardiovascular disease, and fertility and adult cardiovascular health are linked, the association between early-life cardiovascular risk and female infertility has not been studied. METHODS: A total of 1799 women participated in the Babies substudy of the Bogalusa Heart Study. Systolic blood pressure (SBP), diastolic blood pressure (DBP), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides, glucose, and insulin were age-standardized and examined as predictors of self-reported fertility difficulties using multivariable logistic regression with adjustment for confounders...
June 13, 2018: Pediatric Research
Richard Piet, Bruna Kalil, Tim McLennan, Robert Porteous, Katja Czieselsky, Allan E Herbison
A population of kisspeptin-GABA co-expressing neurons located in the rostral periventricular area of the third ventricle (RP3V) is believed to activate gonadotropin-releasing hormone (GnRH) neurons to generate the luteinizing hormone (LH) surge triggering ovulation. Selective optogenetic activation of RP3V kisspeptin (RP3VKISS ) neurons in female mice for >30s and ≥10Hz in either a continuous or bursting mode was found to reliably generate a delayed and long-lasting activation of GnRH neuron firing in brain slices...
June 13, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Zahra Shahraki, Fatemeh Davari Tanha, Mahsa Ghajarzadeh
BACKGROUND: Women suffering from infertility are at higher risk of experiencing psychological problems. Sexual function and sexual related quality of life is not considered as it should be. We designed this study to assess depression, sexual function and sexual quality of life in Iranian women with infertility. METHODS: Two hundred and sixty four individuals participated in the study (115 healthy controls, 78 with primary and 71 with secondary infertility). All participants were asked to fill a valid and reliable Persian versions of BDI (Beck depression inventory), FSFIS (Female Sexual Function Index) and sexual quality of life-Female (SQOL-F) questionnaires...
June 14, 2018: BMC Women's Health
Z Li, A Y Wang, M Bowman, K Hammarberg, C Farquhar, L Johnson, N Safi, E A Sullivan
STUDY QUESTION: What is the cumulative live birth rate following ICSI cycles compared with IVF cycles for couples with non-male factor infertility? SUMMARY ANSWER: ICSI resulted in a similar cumulative live birth rate compared with IVF for couples with non-male factor infertility. WHAT IS KNOWN ALREADY: The ICSI procedure was developed for couples with male factor infertility. There has been an increased use of ICSI regardless of the cause of infertility...
June 12, 2018: Human Reproduction
Can Dai, Liang Hu, Fei Gong, Yueqiu Tan, Sufen Cai, Shuoping Zhang, Jing Dai, Changfu Lu, Jing Chen, Yongzhe Chen, Guangxiu Lu, Juan Du, Ge Lin
PURPOSE: The oocyte-borne genetic causes leading to fertilization failure are largely unknown. We aimed to identify novel human pathogenic variants (PV) and genes causing fertilization failure. METHODS: We performed exome sequencing for a consanguineous family with a recessive inheritance pattern of female infertility characterized by oocytes with a thin zona pellucida (ZP) and fertilization failure in routine in vitro fertilization. Subsequent PV screening of ZP2 was performed in additional eight unrelated infertile women whose oocytes exhibited abnormal ZP and similar fertilization failure...
June 12, 2018: Genetics in Medicine: Official Journal of the American College of Medical Genetics
Mila W Reginatto, Bartira M Pizarro, Roberto A Antunes, Ana C A Mancebo, Luísa Hoffmann, Pâmela Fernandes, Patrícia Areas, Maria I Chiamolera, Rosane Silva, Maria do Carmo Borges de Souza, Enrrico Bloise, Tânia M Ortiga-Carvalho
Purpose: Calcitriol, or 1,25-hydroxycholecalciferol, is the active form of vitamin D. It binds and activates vitamin D receptor (VDR). Infertility and defective folliculogenesis have been observed in female vdr- knockout mice; however, whether VDR polymorphisms affect human ovarian responses to controlled ovarian stimulation (COS) remains unclear. We hypothesized that VDR polymorphisms are associated with infertility and COS responses. Thus, we evaluated the association between the TaqI, BsmI , and FokI VDR polymorphisms and ovarian responses in women undergoing COS...
2018: Frontiers in Endocrinology
Agustin L Lujan, Diego O Croci, Julián A Gambarte Tudela, Antonella D Losinno, Alejandro J Cagnoni, Karina V Mariño, María T Damiani, Gabriel A Rabinovich
Chlamydia trachomatis ( Ct ) constitutes the most prevalent sexually transmitted bacterium worldwide. Chlamydial infections can lead to severe clinical sequelae including pelvic inflammatory disease, ectopic pregnancy, and tubal infertility. As an obligate intracellular pathogen, Ct has evolved multiple strategies to promote adhesion and invasion of host cells, including those involving both bacterial and host glycans. Here, we show that galectin-1 (Gal1), an endogenous lectin widely expressed in female and male genital tracts, promotes Ct infection...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
Amanda J Adeleye, Lusine Aghajanova, Chia-Ning Kao, Marcelle I Cedars, Mark V Sauer
OBJECTIVE: To report differences in ovarian stimulation outcomes in women using a levonorgestrel-releasing intrauterine device (LNG-IUD). DESIGN: Retrospective cohort study. SETTING: University-based infertility practice. PATIENT(S): Female patients pursuing either social oocyte cryopreservation or oocyte donation. INTERVENTION(S): Chart review of all female patients presenting from January 1, 2012, to June 30, 2017, for social oocyte cryopreservation or oocyte donation...
June 6, 2018: Fertility and Sterility
Heng Wang, Yong Pu, Lei Luo, Yunsheng Li, Yunhai Zhang, Zubing Cao
Excessive secretion of androgens including androstenedione and testosterone in theca cells frequently causes female infertility in mammals. Melatonin is a potent inhibitor of androgen production in gonadal cells of several species in a membrane receptor-dependent manner. However, the function of melatonin in steroidogenesis of porcine theca cells remains unclear. Here we report that melatonin inhibits androgen biosynthesis independently of its membrane receptors in pigs. Using flow cytometry, immunofluorescence and RT-PCR we showed that the vast majority of cells isolated from the theca layer of antral follicles are indeed theca cells...
June 1, 2018: Theriogenology
Vijay Boggula, Israel Hanukoglu, Ron Sagiv, Yehoshua Enuka, Aaron Hanukoglu
Pseudohypoaldosteronism type 1 (PHA) is a syndrome of unresponsiveness to aldosterone. The severe form of this disease results from mutations in the genes that encode for the epithelial sodium channel subunits, SCNN1A, SCNN1B, and SCNN1G. A PHA patient under our care failed to conceive after many years and IVF trials. Our earlier studies had shown that ENaC is expressed in the female reproductive tract. We hypothesized that a defective ENaC expression may be responsible for the infertility of the patient. To test this hypothesis we examined ENaC expression in endometrial Pipelle biopsy samples from three healthy women and the PHA patient with an Arg508X mutation in the SCNN1A gene...
June 6, 2018: Journal of Steroid Biochemistry and Molecular Biology
Aditi P Chaudhari, Kaustubh Mazumdar, Pooja Deepak Mehta
Background: Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder among women of reproductive age. Symptoms include amenorrhea, hirsutism, infertility, obesity, acne vulgaris, and androgenic alopecia. PCOS is a stigmatizing condition that affects a woman's identity, mental health and quality of life (QOL). This aspect has not received adequate attention in India. Aims and Objectives: (1) To study the prevalence of anxiety and depression among women suffering from PCOS (2) To determine if symptoms of PCOS were associated with psychiatric morbidity, and (3) To determine the impact of psychiatric morbidity on the QOL...
May 2018: Indian Journal of Psychological Medicine
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