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Katharina Rathmann, Max Herke, Klaus Hurrelmann, Matthias Richter
OBJECTIVES: The aim of this study was to examine the associations between features of class climate and school wellbeing, based on self-rated health and reports of absence from school due to illness among adolescents in secondary schools, by using data from the German National Educational Panel Study (NEPS). METHODS: Data was obtained from the National Educational Panel Study (NEPS). The sample includes (n=7,348) seventh grade students in regular schools (Starting Cohort 3, Wave 3, 2012)...
March 14, 2018: Das Gesundheitswesen
Xiaolin Zhang, Penghui Zhai, Jianhui Huang, Xiang Zhao, Kuanhu Dong
Water use efficiency (WUE) is an important indicator of ecosystem functioning but how ecosystem WUE responds to climate change including precipitation and nitrogen (N) deposition increases is still unknown. To investigate such responses, an experiment with a randomized block design with water (spring snowfall or summer water addition) and nitrogen addition was conducted in a temperate steppe of northern China. We investigated net ecosystem CO2 production (NEP), gross ecosystem production (GEP) and evapotranspiration (ET) to calculate ecosystem WUE (WUEnep = NEP/ET or WUEgep = GEP/ET) under spring snow and summer water addition with or without N addition from 2011 to 2013...
2018: PloS One
Alyne Marem, Debora N Okamoto, Lilian C G Oliveira, Diego M Ruiz, Roberto A Paggi, Marcia Y Kondo, Iuri E Gouvea, Maria A Juliano, Rosana E de Castro, Luiz Juliano, Marcelo Y Icimoto
Nep (Natrialba magadii extracellular protease) is a halolysin-like peptidase secreted by the haloalkaliphilic archaeon Natrialba magadii. Many extracellular proteases have been characterized from archaea to bacteria as adapted to hypersaline environments retaining function and stability until 4.0 M NaCl. As observed in other secreted halolysins, this stability can be related to the presence of a C-terminal extension (CTE) sequence. In the present work, we compared the biochemical properties of recombinant Nep protease with the truncated form at the 134 amino acids CTE (Nep∆CTE), that was more active in 4 M NaCl than the non-truncated wild type enzyme...
March 7, 2018: International Journal of Biological Macromolecules
Jung Gi Lee, Jae Ha Ryu, Seon-Myung Kim, Moon-Young Park, San-Ho Kim, Young G Shin, Jong-Woo Sohn, Ha Hyung Kim, Zee-Yong Park, Jae Young Seong, Jae Il Kim
Exendin-4, a 39 amino acid peptide isolated from the saliva of the Gila monster, plays an important role in regulating glucose homeostasis, and is used clinically for the treatment of type 2 diabetes. Exendin-4 shares 53% sequence identity with the incretin hormone glucagon-like peptide 1 (GLP-1) but, unlike GLP-1, is highly resistant to proteolytic enzymes such as dipeptidyl peptidase IV (DPP-IV) and neutral endopeptidase 24.11 (NEP 24.11). Herein, we focused on the structure and function of the C-terminal Trp-cage of exendin-4, and suggest that it may be structurally required for resistance to proteolysis by NEP 24...
March 6, 2018: Biochemical Pharmacology
N-N An, J Shawn, J-P Peng, M-D Wu, L-G Huang
OBJECTIVE: Wilms' tumor (WT) is the most common malignant tumor in the children's urogenital system. MiR-190b was found to participate in the development and progression of several cancers. However, the molecular mechanism of miR-190b in WT is still unclear. PATIENTS AND METHODS: We detected the miR-190b in WT tissue samples compared to adjacent normal samples as well as in WT patients' blood sample compared to normal volunteers using qRT-PCR. With over-expression and knockdown of miR-190b in WT-derived cell line SK-NEP-1, we next studied cell proliferation, cell circle, apoptosis, invasion and migration abilities change caused by miR-190b ectopic expression...
February 2018: European Review for Medical and Pharmacological Sciences
Xiuyun Jiang, Huaicheng Li
Introduction: Wilms' tumor (WT), the most common childhood tumor, occurs in sporadic or familial forms. Recent findings reported that abnormal expression in microRNA (miRNA) suggests an important role of miRNAs during WT progress. MiRNAs are endogenous short-chain noncoding RNAs, which have been reported as key biomarkers for detecting tumor onset and progression. However, the functional role of miR-1180 in WT has remained unknown. Materials and methods: MTT and clonogenic survival assays were used to detect WT cell proliferation...
2018: OncoTargets and Therapy
A O Golovko, O N Koroleva, V L Drutsa
Influenza A virus nuclear export protein NEP (NS2, 14.4 kDa) plays a key role in various steps of the virus life cycle. Highly purified protein preparations are required for structural and functional studies. In this study, we designed a series of Escherichia coli plasmid constructs for highly efficient expression of the NEP gene under control of the constitutive trp promoter. An efficient method for extraction of NEP from inclusion bodies based on dodecyl sulfate treatment was developed. Preparations of purified NEP with either N- or C-terminal (His)6 -tag were obtained using Ni-NTA agarose affinity chromatography with yield of more than 20 mg per liter of culture...
December 2017: Biochemistry. Biokhimii︠a︡
Sérgio M de Almeida, Bin Tang, Clea E Ribeiro, Indianara Rotta, Florin Vaida, Mauro Piovesan, Meire S Batistela Fernandes, Scott Letendre, Michael Potter, Ronald J Ellis
OBJECTIVE-: Neprilysin (NEP) is the dominant Aβ peptide-degrading enzyme in the brain. HIV-1 subtype B Tat protein is known to interfere with NEP function, but whether this is true of HIV-1C Tat, which has a defective chemokine motif, is not known. This study aimed to analyze the impact of HIV subtype on NEP-mediated cleavage of Aβ by comparing CSF and serum levels of NEP between HIV+ (27 patients with HIV-1B and 26 with HIV-1C), healthy HIV- controls (n=13); and patients with Alzheimer's disease (AD,n=24)...
February 21, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
Juan A Delgado-Notario, Jesus E Velazquez-Perez, Yahya M Meziani, Kristel Fobelets
Plasma waves in gated 2-D systems can be used to efficiently detect THz electromagnetic radiation. Solid-state plasma wave-based sensors can be used as detectors in THz imaging systems. An experimental study of the sub-THz response of II-gate strained-Si Schottky-gated MODFETs (Modulation-doped Field-Effect Transistor) was performed. The response of the strained-Si MODFET has been characterized at two frequencies: 150 and 300 GHz: The DC drain-to-source voltage transducing the THz radiation (photovoltaic mode) of 250-nm gate length transistors exhibited a non-resonant response that agrees with theoretical models and physics-based simulations of the electrical response of the transistor...
February 10, 2018: Sensors
J P Cruz, F Alshammari, R F D Felicilda-Reynaldo
AIM: This study aimed to investigate the predictors of Saudi nursing students' attitudes towards the environment and sustainability in health care. BACKGROUND: With rising temperature and decreasing annual rainfall, Saudi Arabia is threatened by the harmful effects of climate change on its population. In response to these threats, the Ministry of Health adapted sustainable development and environmental preservation in their National E-Health strategy. INTRODUCTION: To implement these policies successfully, healthcare practitioners should be educated on how climate change could impact human health negatively...
February 9, 2018: International Nursing Review
Katharina Rathmann, Max G Herke, Klaus Hurrelmann, Matthias Richter
The aim of this study is to examine the impact of class-level class climate on school-aged children's life satisfaction. Data was derived from the German National Educational Panel Study (NEPS) using sixth grade school-aged children (n = 4,764, 483 classes). Class climate includes indicators of teachers' care and monitoring, demands, interaction, autonomy, as well as school-aged children's attitudes towards schoolwork at the class- and individual-level. Results showed that individual perceived class climate in terms of teachers' care and monitoring and autonomy was positively related to life satisfaction, whereas school-related demands were related to lower life satisfaction...
2018: PloS One
Ines Pagel-Langenickel
Natriuretic peptides (NP) are widely recognized as key regulators of blood pressure, water and salt homeostasis. In addition, they play a critical role in physiological cardiac growth and mediate a variety of biological effects including antiproliferative and anti-inflammatory effects in other organs and tissues. The cardiac release of NPs ANP and BNP represents an important compensatory mechanism during acute and chronic cardiac overload and during the pathogenesis of heart failure where their actions counteract the sustained activation of renin-angiotensin-aldosterone and other neurohormonal systems...
February 7, 2018: Advances in Experimental Medicine and Biology
Xu Wang, Yue Wang, Song Yu, Lu Ren
OBJECTIVE: To observe the effect of electroacupuncture (EA) on the levels of Aβ 1-40 and Aβ 1-42 and neprilysin (NEP) protein in the temporal cortex of SAMP 8 mice with Alzheimer's disease (AD), so as to explore its mechanism underlying improvement of AD based on the theory of "mutual assistance of kidney and brain" in traditional Chinese medicine. METHODS: Eighteen male SAMP 8 mice were randomly assigned to 3 groups: model, EA and medication (memantine, n=6/group), and 6 male homologous SAMR 1 mice were used as the normal control group...
January 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
Yong-Chul Kim, Anyela Marcela Castañeda, Chang-Soon Lee, Hyun-Seung Jin, Keun Seok Park, Jee Youn Moon
BACKGROUND AND OBJECTIVES: Lidocaine infusion therapy (LIT) is an effective treatment for relieving neuropathic pain (NeP). However, it remains unclear whether pain relief can be sustained through repeated lidocaine infusions. This study aimed to determine whether repeated intravenous administration of low-dose lidocaine could provide prolonged pain relief in patients with specific NeP conditions. METHODS: This is a prospective, randomized, double-blind, placebo-controlled, parallel study...
January 29, 2018: Regional Anesthesia and Pain Medicine
Mónica J B Amorim, Sijie Lin, Karsten Schlich, José M Navas, Andrea Brunelli, Nicole Neubauer, Klaus Vilsmeier, Anna L Costa, Andreas Gondikas, Tian Xia, Liliana Galbis, Elena Badetti, Antonio Marcomini, Danail Hristozov, Frank von der Kammer, Kerstin Hund-Rinke, Janeck J Scott-Fordsmand, André Nel, Wendel Wohlleben
Nanoenabled products (NEPs) have numerous outdoor uses in construction, transportation or consumer scenarios, and there is evidence that their fragments are released in the environment at low rates. We hypothesized that the lower surface availability of NEPs fragment reduced their environmental effects with respect to pristine nanomaterials. This hypothesis was explored by testing fragments generated by intentional micronisation ("the SUN approach"; Nowack et al. Meeting the Needs for Released Nanomaterials Required for Further Testing: The SUN Approach...
January 27, 2018: Environmental Science & Technology
Huang Huang Luo, Cheng Wu, Peng Hu, Yang Fang Wu, Dong Dong Zhang, Si Yan Liu, Guang Mei Jiang, Yao Xu, Yue Wu, Jing Jing Wang, Fei Fei Liu, Wei Wei, Bo Hu
C-type natriuretic peptide (CNP) is regarded as a local, paracrine hormone to regulate vascular tone and cell proliferation. Although several in vivo studies have documented that CNP exerts the inhibitory effects on mesangial cells (MCs) proliferation and collagen production, a limited number of studies exist about the resistance of CNP to MCs proliferation in vitro. Besides, whether its receptor signaling and neutral endopeptidase (NEP) are involved remains unclear. In the present study, human MCs were incubated in serum-containing medium in the absence or presence of CNP (0, 10 and 100 pM) for 24, 48 and 72 hours, respectively...
January 23, 2018: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
Shihui Fu, Ping Ping, Fengqi Wang, Leiming Luo
As a family of hormones with pleiotropic effects, natriuretic peptide (NP) system includes atrial NP (ANP), B-type NP (BNP), C-type NP (CNP), dendroaspis NP and urodilatin, with NP receptor-A (guanylate cyclase-A), NP receptor-B (guanylate cyclase-B) and NP receptor-C (clearance receptor). These peptides are genetically distinct, but structurally and functionally related for regulating circulatory homeostasis in vertebrates. In humans, ANP and BNP are encoded by NP precursor A (NPPA) and NPPB genes on chromosome 1, whereas CNP is encoded by NPPC on chromosome 2...
2018: Journal of Biological Engineering
Hee Jeong Yoon, Xiaowei Zhang, Min Gyeong Kang, Gyeong Jin Kim, Sun Young Shin, Sang Hong Baek, Bom Nae Lee, Su Jung Hong, Jun Tae Kim, Kwonho Hong, Hojae Bae
To overcome the drawbacks of conventional drug delivery system, nanoemulsion have been developed as an advanced form for improving the delivery of active ingredients. However, safety evaluation is crucial during the development stage before the commercialization. Therefore, the aim of this study was to evaluate the cytotoxicity of two types of newly developed nanoemulsions. Turmeric extract-loaded nanoemulsion powder-10.6 (TE-NEP-10.6, high content of artificial surfactant Tween 80), which forms the optimal nanoemulsion, and the TE-NEP-8...
January 17, 2018: International Journal of Molecular Sciences
Kenichi Nagata, Tatsuo Mano, Shigeo Murayama, Takaomi C Saido, Atsushi Iwata
Neprilysin (NEP), a membrane-bound metalloprotease, has been shown to play an essential role in the clearance of amyloid beta (Aβ) peptides. Previous studies have reported that NEP expression is downregulated in the normal aging brain as well as in the Alzheimer's disease (AD) brain, providing evidence that the downregulation of NEP expression contributes to the age-dependent deposition of Aβ-containing plaques, a pathological hallmark of AD. However, the mechanisms underlying the downregulation remain unclear...
January 12, 2018: Neuroscience Letters
Qing-Bo Yu, Tuan-Tuan Zhao, Lin-Shan Ye, Ling Cheng, Ying-Qian Wu, Chao Huang, Zhong-Nan Yang
In higher plant chloroplasts, the plastid-encoded RNA polymerase (PEP) consists of four catalytic subunits and numerous nuclear-encoded accessory proteins, including pTAC10, an S1-domain-containing protein. In this study, pTAC10 knockout lines were characterized. Two ptac10 mutants had an albino phenotype and severely impaired chloroplast development. The pTAC10 genomic sequence fused to a four-tandem MYC tag driven by its own promoter functionally complemented the ptac10-1 mutant phenotype. pTAC10 was present in both the chloroplast stroma and thylakoids...
January 12, 2018: Photosynthesis Research
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