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https://www.readbyqxmd.com/read/28453638/etg-ets-in-serum-by-uhplc-ms-ms-in-suspected-sexual-assault-cases
#1
Solfrid Hegstad, Arne Helland, Cecilie Hagemann, Olav Spigset
A method including semi-automated extraction of ethyl glucuronide (EtG) and ethyl sulfate (EtS) from serum followed by ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS-MS) has been developed and validated. Sample preparation prior to UHPLC-MS-MS analysis consisted of protein precipitation and filtration through a phospholipid removal plate. Chromatography was achieved using an HSS T3 column and gradient elution with formic acid in water in combination with methanol. The mass spectrometer was monitored in the negative mode with multiple reaction monitoring...
April 27, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28453630/the-effects-of-cpg-densities-around-transcription-start-sites-on-sex-biased-gene-expression-in-poecilia-reticulata
#2
Katsunori Tamagawa, Takashi Makino, Masakado Kawata
Since most genes are shared between females and males, DNA methylation is assumed to play a crucial role in sex-biased gene expression. DNA methylation exclusively occurs at CpG dinucleotides, and therefore, we would expect that CpG density around transcription start sites (TSSs) relate to sex-biased gene expression. Here we investigated the relationship between CpG densities around TSSs and the ratio of gene expression levels between sexes in the guppy (Poecilia reticulata), which displays remarkable sexual dimorphisms...
April 26, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28453403/high-rate-of-hepatitis-b-virus-surface-antigenemia-among-people-living-with-hiv-aids-in-kakuri-kaduna-state-north-west-nigeria
#3
Cornelius Arome Omatola, Bernard Anyebe Onoja, Temitope Thomas
Globally, increased incidence of liver disease caused by hepatitis B virus (HBV) is responsible for high morbidity and mortality among human immunodeficiency virus (HIV)-infected individuals. This is because both viruses share common routes of transmission. We determined prevalence of HBV-HIV coinfection and the influence of some risk factors on concomitant infection among people living with HIV in a treatment center in Kakuri, Kaduna State. Two hundred consenting individuals with HIV infection participated in the study...
April 28, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28448483/trends-in-repeat-births-and-use-of-postpartum-contraception-among-teens-united-states-2004-2015
#4
Deborah L Dee, Karen Pazol, Shanna Cox, Ruben A Smith, Katherine Bower, Martha Kapaya, Amy Fasula, Ayanna Harrison, Charlan D Kroelinger, Denise D'Angelo, Leslie Harrison, Emilia H Koumans, Nikki Mayes, Wanda D Barfield, Lee Warner
Teen* childbearing (one or more live births before age 20 years) can have negative health, social, and economic consequences for mothers and their children (1). Repeat teen births (two or more live births before age 20 years) can constrain the mother's ability to take advantage of educational and workforce opportunities (2), and are more likely to be preterm or of low birthweight than first teen births (3). Despite the historic decline in the U.S. teen birth rate during 1991-2015, from 61.8 to 22.3 births per 1,000 females aged 15-19 years (4), many teens continue to have repeat births (3)...
April 28, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28447919/postpartum-dyspareunia-and-sexual-functioning-a-prospective-cohort-study
#5
Liesbet Lagaert, Steven Weyers, Helena Van Kerrebroeck, Els Elaut
OBJECTIVES: Sexual functioning is an important concern for women in the postpartum period. The aim of this research was to investigate the prevalence and determinants of dyspareunia and sexual dysfunction before and after childbirth. METHODS: Between November 2013 and April 2014, 109 women in their third trimester of pregnancy were enrolled in a prospective cohort study at Ghent University Hospital. Dyspareunia, sexual functioning and quality of life (QOL) were evaluated at enrolment and again 6 weeks and 6 months postpartum...
April 27, 2017: European Journal of Contraception & Reproductive Health Care
https://www.readbyqxmd.com/read/28447815/development-of-the-healthcare-triggering-questionnaire-in-adult-sexual-abuse-survivors
#6
Julie B Schnur, William F Chaplin, Kiran Khurshid, Jazmin N Mogavero, Rachel E Goldsmith, Young-Sun Lee, Leib Litman, Guy H Montgomery
OBJECTIVE: One in 4 women and 1 in 10 men in the United States are survivors of sexual abuse (SA). For these survivors, healthcare experiences may trigger memories, thoughts, feelings or sensations related to this past abuse. Such triggering can be associated with negative responses to healthcare (e.g., anxiety, avoidance). However, to date, no healthcare triggering assessment tool exists. Therefore, the study goal was to describe the prevalence of healthcare triggering, to develop a brief Healthcare Triggering Questionnaire (HTQ), and to examine its initial validity...
April 27, 2017: Psychological Trauma: Theory, Research, Practice and Policy
https://www.readbyqxmd.com/read/28447367/sex-hormones-function-as-sex-attractant-pheromones-in-house-mice-and-brown-rats
#7
Stephen Takacs, Regine Gries, Gerhard J Gries
Sex hormones of mammals control the expression of sexual characteristics and bodily functions. The male hormone testosterone and the female hormones progesterone and estradiol are known to occur in urine markings of mice. Herein, we show that all three hormones are also present in urine of brown rats, and that they are effective sexual communication signals (pheromones) that elicit attraction behavior of prospective mates in both brown rats and house mice. When added as lures to trap boxes in field experiments, synthetic testosterone, e...
April 26, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28447195/salivary-mir-16-mir-191-and-mir-223-intuitive-indicators-of-dominant-ovarian-follicles-in-buffaloes
#8
Prashant Singh, Naresh Golla, Pankaj Singh, Vijay Simha Baddela, Subhash Chand, Rubina Kumari Baithalu, Dheer Singh, Suneel Kumar Onteru
Estrus or sexual receptivity determination is utmost important for efficient breeding programs for female buffaloes. Prominent estrus behavioral symptoms are the result of several molecular and neuroendocrine events involving the ovary and the brain. Expression of estrus behavior is poor in buffaloes during the summer season. Hence, the discovery of biomarkers specific to the estrus stage or its related ovarian events, like the presence of dominant ovarian follicle, is helpful for developing an easy estrus determination method...
April 26, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28445684/patient-staffing-and-health-centre-factors-associated-with-annual-testing-for-sexually-transmissible-infections-in-remote-primary-health-centres
#9
Belinda Hengel, Handan Wand, James Ward, Alice Rumbold, Linda Garton, Debbie Taylor-Thomson, Bronwyn Silver, Skye McGregor, Amalie Dyda, Jacqueline Mein, Janet Knox, Lisa Maher, John Kaldor, Rebecca Guy
Background: In high-incidence Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) settings, annual re-testing is an important public health strategy. Using baseline laboratory data (2009-10) from a cluster randomised trial in 67 remote Aboriginal communities, the extent of re-testing was determined, along with the associated patient, staffing and health centre factors. Methods: Annual testing was defined as re-testing in 9-15 months (guideline recommendation) and a broader time period of 5-15 months following an initial negative CT/NG test...
April 27, 2017: Sexual Health
https://www.readbyqxmd.com/read/28445139/pannexin1-knockout-and-blockade-reduces-ischemic-stroke-injury-in-female-but-not-in-male-mice
#10
Moises Freitas-Andrade, John F Bechberger, Brian A MacVicar, Victor Viau, Christian C Naus
The membrane channel Pannexin 1 (Panx1) mediates apoptotic and inflammatory signaling cascades in injured neurons, responses previously shown to be sexually dimorphic under ischemic conditions. We tested the hypothesis that Panx1 plays an underlying role in mediating sex differences in stroke outcome responses. Middle-aged, 8-9 month old male and female wild type and Panx1 KO mice were subjected to permanent middle cerebral artery (MCA) occlusion, and infarct size and astrocyte and microglia activation were assessed 4 days later...
April 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28444386/coevolution-with-pollinating-resin-midges-led-to-resin-filled-nurseries-in-the-androecia-gynoecia-and-tepals-of-kadsura-schisandraceae
#11
Shi-Xiao Luo, Ting-Ting Liu, Fei Cui, Zi-Yin Yang, Xiao-Ying Hu, Susanne S Renner
Background and Aims: Resin is a defence against herbivores and a floral reward in a few African and South American species whose bee pollinators collect it for nest construction. Here we describe a new role for floral resin from the Asian genus Kadsura (Schisandraceae). Kadsura tepals tightly cover a globe formed by carpels (in females) or near-fused stamens with fleshy connectives (in male flowers of most, but not all species). Methods: We carried out field observations at four sites in China and used pollinator behavioural assays, chemical analyses and time-calibrated insect and plant phylogenies to investigate the specificity of the interactions and their relationship to floral structure...
April 21, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28444197/understanding-the-linkages-between-social-safety-nets-and-childhood-violence-a-review-of-the-evidence-from-low-and-middle-income-countries
#12
Amber Peterman, Anastasia Naomi Neijhoft, Sarah Cook, Tia M Palermo
As many as one billion children experience violence every year, and household- and community-level poverty are among the risk factors for child protection violations. Social safety nets (SSNs) are a main policy tool to address poverty and vulnerability, and there is substantial evidence demonstrating positive effects on children's health and human capital. This paper reviews evidence and develops a framework to understand linkages between non-contributory SSNs and the experience of childhood emotional, physical and sexual violence in low- and middle-income countries...
April 22, 2017: Health Policy and Planning
https://www.readbyqxmd.com/read/28444173/tac1-signaling-is-required-for-sexual-maturation-and-responsiveness-of-gnrh-neurons-to-kisspeptin-in-the-male-mouse
#13
Caroline A Maguire, Yong Bhum Song, Min Wu, Silvia León, Rona S Carroll, Meenakshi Alreja, Ursula B Kaiser, Víctor M Navarro
The tachykinins substance P (SP) and neurokinin A (NKA) (Tac1) have emerged as novel regulators of kisspeptin/GnRH release. Recently, we documented that SP modulates reproductive function in the female mouse. Here, we extended this characterization to the male mouse. Tac1-/- male mice showed delayed puberty onset. They also presented significantly decreased expression levels of Pdyn (dynorphin) and Nos1 (nitric oxide synthase) in the mediobasal hypothalamus and elevated Gnrh1 levels. Unexpectedly, the response of Tac1-/- mice to central kisspeptin or senktide (neurokinin B receptor -NK3R- agonist) administration was significantly decreased compared with controls, despite the preserved ability of GnRH neurons to stimulate LH release as demonstrated by central NMDA administration, suggesting a deficit at the GnRH neuron level...
April 21, 2017: Endocrinology
https://www.readbyqxmd.com/read/28443629/differences-in-peripheral-sensory-input-to-the-olfactory-bulb-between-male-and-female-mice
#14
Marley D Kass, Lindsey A Czarnecki, Andrew H Moberly, John P McGann
Female mammals generally have a superior sense of smell than males, but the biological basis of this difference is unknown. Here, we demonstrate sexually dimorphic neural coding of odorants by olfactory sensory neurons (OSNs), primary sensory neurons that physically contact odor molecules in the nose and provide the initial sensory input to the brain's olfactory bulb. We performed in vivo optical neurophysiology to visualize odorant-evoked OSN synaptic output into olfactory bub glomeruli in unmanipulated (gonad-intact) adult mice from both sexes, and found that in females odorant presentation evoked more rapid OSN signaling over a broader range of OSNs than in males...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28443134/characterization-of-x-chromosome-gene-expression-in-bovine-blastocysts-derived-by-in-vitro-fertilization-and-somatic-cell-nuclear-transfer
#15
Byungkuk Min, Jung Sun Park, Kyuheum Jeon, Yong-Kook Kang
To better understand X-chromosome reactivation (XCR) during early development, we analyzed transcriptomic data obtained from bovine male and female blastocysts derived by in-vitro fertilization (IVF) or somatic-cell nuclear transfer (SCNT). We found that X-linked genes were upregulated by almost two-fold in female compared with male IVF blastocysts. The upregulation of X-linked genes in female IVFs indicated a transcriptional dimorphism between the sexes, because the mean autosomal gene expression levels were relatively constant, regardless of sex...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28442935/new-developments-in-the-treatment-of-hypoactive-sexual-desire-disorder-a-focus-on-flibanserin
#16
REVIEW
Christopher J Jayne, Michael J Heard, Sarah Zubair, Dustie L Johnson
The objective of the authors is to highlight the historical complexities for the diagnosis and treatment of hypoactive sexual desire disorder (HSDD) with a focus on Flibanserin. A systematic review of the medical literature published in PubMed using the search terms HSDD and Flibanserin was conducted. Each author reviewed the results of the systematic review for articles to include in this study. HSDD is defined as a persistent or recurrent deficiency or absence of sexual fantasies and desire for sexual activity causing marked distress or interpersonal difficulty that is not better accounted for by another diagnosis...
2017: International Journal of Women's Health
https://www.readbyqxmd.com/read/28442805/proton-pump-inhibitors-are-associated-with-a-reduced-likelihood-for-sexually-transmitted-diseases-in-women-in-the-emergency-department
#17
Johnathan Michael Sheele, Nathan Morris, Donald Byars, Frank Counselman
BACKGROUND: Proton pump inhibitors (PPIs) have been shown in cell culture to kill Trichomonas vaginalis (TV) at lower half maximal inhibitory concentration values than metronidazole (Flagyl), the most common medication used to treat the infection. However, there have been no previous clinical investigations to determine if PPIs are associated with reduced risk for TV. MATERIALS AND METHODS: We examined the records of female patients who received testing in the emergency department for TV, Neisseria gonorrhoea (GC), and Chlamydia trachomatis (CT) between 2010 and 2014 at two academic medical centers to determine if PPI and histamine type 2 receptor antagonist (H2RA) drugs were associated with TV and GC/CT infections...
January 2017: Indian Journal of Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28442802/study-of-prevalence-of-sexually-transmitted-infections-human-immunodeficiency-virus-and-condom-use-among-male-to-female-transgender-a-retrospective-analysis-from-a-tertiary-care-hospital-in-chennai
#18
Subhash Dasarathan, S Kalaivani
CONTEXT: A "Transgender" person is one who dresses as, desires to be, has undergone surgery to become or identifies with opposite sex. They have a higher risk of sexually transmitted infection (STI) due to a combination of biological and social reasons. They have risky sexual behaviors but low-risk perception. AIMS: The aim of this study is to determine the prevalence of STI/human immunodeficiency virus (HIV) in transgender (TG) and association with condom use. SETTINGS AND DESIGN: A retrospective study of 82 male-to-female TGs attending our sexually transmitted disease (STD) clinic from 2011 to 2014 was undertaken...
January 2017: Indian Journal of Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28442798/knowledge-and-attitude-about-sexually-transmitted-infections-other-than-hiv-among-college-students
#19
Nagesh Tumkur Subbarao, A Akhilesh
BACKGROUND: Sexually transmitted infections (STIs) are the infections which are mainly transmitted through sexual intercourse. Young individuals in the age group of 16 to 24 years are considered to be at more risk for STIs compared to older adults. Young individuals are more likely to practice unprotected sex and have multiple sexual partners. If the STIs are not treated adequately, it can lead to various complications.Most of the people may be aware about HIV/AIDs because of the awareness created by media and the government programs, however knowledge about STIs other than HIV/AIDS is low in the developing countries...
January 2017: Indian Journal of Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28442797/pre-and-post-sexual-exposure-prophylaxis-of-hiv-an-update
#20
REVIEW
Yogesh S Marfatia, Sheethal K Jose, Reema R Baxi, Ruchi J Shah
Pitfalls in current HIV prevention strategies include late HIV testing, vulnerability among youth and females; lack of emphasis on treatment, low acceptance of circumcision, and nonavailability of protective vaccines. Continuing high-risk sexual behavior, forceful sex, coercive and nonconsensual sex, rape, and unprotected sexual activities make women the most vulnerable to acquisition of sexually transmitted infection/HIV and necessitates a more radical approach of prevention in high-risk individuals who do not have HIV...
January 2017: Indian Journal of Sexually Transmitted Diseases
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