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Dendrobium officinale

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https://www.readbyqxmd.com/read/27877182/insights-from-the-cold-transcriptome-and-metabolome-of-dendrobium-officinale-global-reprogramming-of-metabolic-and-gene-regulation-networks-during-cold-acclimation
#1
Zhi-Gang Wu, Wu Jiang, Song-Lin Chen, Nitin Mantri, Zheng-Ming Tao, Cheng-Xi Jiang
Plant cold acclimation (CA) is a genetically complex phenomenon involving gene regulation and expression. Little is known about the cascading pattern of gene regulatroy network and the link between genes and metabolites during CA. Dendrobium officinale (DOKM) is an important medicinal and ornamental plant and hypersensitive to low temperature. Here, we used the large scale metabolomic and transcriptomic technologies to reveal the response to CA in DOKM seedlings based on the physiological profile analyses. Lowering temperature from 4 to -2°C resulted in significant increase (P < 0...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27814359/whole-transcriptome-selection-and-evaluation-of-internal-reference-genes-for-expression-analysis-in-protocorm-development-of-dendrobium-officinale-kimura-et-migo
#2
Hongqiang An, Qiankun Zhu, Wei Pei, Jing Fan, Yi Liang, Yihui Cui, Nan Lv, Wanjun Wang
Dendrobium officinale Kimu et Migo has increased many researchers' interest for its high medical and horticultural values and the molecular mechanism of its protocorm development remains unclear. In this study, 19 genes from 26 most stably expressed genes in whole transcriptome of protocorms and 5 housekeeping genes were used as candidate reference genes and screened with 4 application softwares (geNorm, NormFinder, BestKeeper and RefFinder). The results showed that a few reference genes could effectively normalize expression level of specific genes in protocorm development and the optimal top 2 reference genes were ASS and APH1L...
2016: PloS One
https://www.readbyqxmd.com/read/27802903/dendrobium-officinale-kimura-et-migo-attenuates-diabetic-cardiomyopathy-through-inhibiting-oxidative-stress-inflammation-and-fibrosis-in-streptozotocin-induced-mice
#3
Zhihao Zhang, Duoduo Zhang, Mengmeng Dou, Zhubo Li, Jie Zhang, Xiaoyan Zhao
Dendrobium officinale Kimura et Migo (Dendrobium catenatum Lindley), a prized traditional Chinese Medicine, has been used in China and Southeast Asian countries for centuries. The present study was aimed to investigate the effects and the possible mechanisms of the Dendrobium officinale extracts (DOE) on diabetic cardiomyopathy in mice. The diabetic model was induced by intraperitoneal injection of streptozotocin at the dose of 50mg/kg body weight for 5 consecutive days. After 8 weeks treatment of DOE, mice were sacrificed, blood sample and heart tissues were collected...
October 29, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27748908/cardioprotective-potential-of-dendrobium%C3%A2-officinale-kimura-et-migo-against-myocardial-ischemia-in-mice
#4
Meng-Meng Dou, Zhi-Hao Zhang, Zhu-Bo Li, Jie Zhang, Xiao-Yan Zhao
Dendrobium officinale Kimura et Migo has been used for thousands of years to promote body fluid production; however, little is currently known regarding its effects on the heart. The present study aimed to explore the cardioprotective potential of the water extract of Dendrobium officinale Kimura et Migo (DOE) on myocardial ischemia in mice. A mouse model of myocardial ischemia was induced following ligation of the left anterior descending coronary artery. Prior to the operation, mice were administered a vehicle or DOE for 2 weeks...
September 26, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27710025/identification-and-characterization-of-a-chloroplast-targeted-obg-gtpase-in-dendrobium-officinale
#5
Ji Chen, Feng Deng, Mengsheng Deng, Jincheng Han, Jianbin Chen, Li Wang, Shen Yan, Kai Tong, Fan Liu, Mengliang Tian
Bacterial homologous chloroplast-targeted Obg GTPases (ObgCs) belong to the plant-typical Obg group, which is involved in diverse physiological processes during chloroplast development. However, the evolutionarily conserved function of ObgC in plants remains elusive and requires further investigation. In this study, we identified DoObgC from an epiphytic plant Dendrobium officinale and demonstrated the characteristics of DoObgC. Sequence analysis indicated that DoObgC is highly conserved with other plant ObgCs, which contain the chloroplast transit peptide (cTP), Obg fold, G domain, and OCT regions...
October 6, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27429889/element-tracing-of-mineral-matters-in-dendrobium-officinale-using-icp-ms-and-multivariate-analysis
#6
Nannan Zhu, Shen Han, Chunning Yang, Jixu Qu, Zhirong Sun, Wenjie Liu, Xiaomin Zhang
Rare studies have been performed to trace the mineral elements in Dendrobium officinale. In this study, we aim to trace the mineral elements in D. officinale collected from ten geographical locations in China. ICP-MS system was used for simultaneous determination of mineral elements. Principal component analysis was performed using the obtained data in the quantification of mineral contents. Cluster analysis was performed using the Ward's method. Several of essential microelments were detected in D. officinale, including ferrum (Fe), manganese (Mn), zinc (Zn), chromium (Cr), nickel (Ni) and vanadium (V)...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27356377/-correlation-between-functional-groups-and-radical-scavenging-activities-of-acidic-polysaccharides-from-dendrobium
#7
Ying Liao, Wen-yu Yuan, Wen-ke Zheng, Ao-xue Luo, Yi-jun Fan
OBJECTIVE: To compare the radical scavenging activity of five different acidic polysaccharides, and to find the correlation with the functional groups. METHODS: Alkali extraction method and Stepwise ethanol precipitation method were used to extract and concentrate the five Dendrobium polysaccharides, and to determine the contents of sulfuric acid and uronic acid of each kind of acidic polysaccharides, and the scavenging activity to ABTS+ radical and hydroxyl radical...
November 2015: Zhong Yao Cai, Zhongyaocai, Journal of Chinese Medicinal Materials
https://www.readbyqxmd.com/read/27339052/two-strategies-by-epiphytic-orchids-for-maintaining-water-balance-thick-cuticles-in-leaves-and-water-storage-in-pseudobulbs
#8
Shi-Jian Yang, Mei Sun, Qiu-Yun Yang, Ren-Yi Ma, Jiao-Lin Zhang, Shi-Bao Zhang
Epiphytes are an important component of tropical and subtropical flora, and serve vital ecological functions in forest hydrology and nutrient fluxes. However, they often encounter water deficits because there is no direct contact between their roots and the soil. The strategies employed by epiphytes for maintaining water balance in relatively water-limited habitats are not completely understood. In the present study, we investigated the anatomical traits, water loss rates, and physiology of leaves and pseudobulbs of four Dendrobium species with different pseudobulb morphologies to understand the roles of leaf and pseudobulb in maintaining water balance of epiphytic orchids...
2016: AoB Plants
https://www.readbyqxmd.com/read/27248989/purification-characterization-and-biological-activity-of-polysaccharides-from-dendrobium-officinale
#9
Kaiwei Huang, Yunrong Li, Shengchang Tao, Gang Wei, Yuechun Huang, Dongfeng Chen, Chengfeng Wu
Polysaccharide (DOPA) from the stem of D. officinale, as well as two fractions (DOPA-1 and DOPA-2) of it, were isolated and purified by DEAE cellulose-52 and Sephacryl S-300 chromatography, and their structural characteristics and bioactivities were investigated. The average molecular weights of DOPA-1 and DOPA-2 were 394 kDa and 362 kDa, respectively. They were mainly composed of d-mannose, d-glucose, and had a backbone consisting of 1,4-linked β-d-Manp and 1,4-linked β-d-Glcp with O-acetyl groups. Bioactivity studies indicated that both DOPA and its purified fractions (DOPA-1 and DOPA-2) could activate splenocytes and macrophages...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27225227/intestinal-immunomodulating-activity-and-structural-characterization-of-a-new-polysaccharide-from-stems-of-dendrobium-officinale
#10
Song-Zi Xie, Bing Liu, Dan-Dan Zhang, Xue-Qiang Zha, Li-Hua Pan, Jian-Ping Luo
A homogeneous polysaccharide fraction (DOP-W3-b) with a high intestinal immunomodulating activity was obtained from the stems of Dendrobium officinale through a bioactivity-guided sequential isolation procedure based on the screening of Peyer's patch-mediated immunomodulating activity. Oral administration experiments of mice showed that DOP-W3-b could effectively regulate intestinal mucosal immune activity by changing intestinal mucosal structures, promoting the secretions of cytokines from Peyer's patches (PPs) and mesenteric lymph nodes (MLNs), and increasing the production of secretory immunoglobulin A (sIgA) in the lamina propria...
June 15, 2016: Food & Function
https://www.readbyqxmd.com/read/27173310/characterization-of-the-alkaline-neutral-invertase-gene-in-dendrobium-officinale-and-its-relationship-with-polysaccharide-accumulation
#11
F Gao, X F Cao, J P Si, Z Y Chen, C L Duan
Dendrobium officinale is one of the most well-known traditional Chinese medicines, and polysaccharide is its main active ingredient. Many studies have investigated the synthesis and accumulation mechanisms of polysaccharide, but until recently, little was known about the molecular mechanism of how polysaccharide is synthesized because no related genes have been cloned. In this study, we cloned an alkaline/neutral invertase gene from D. officinale (DoNI) by the rapid amplification of cDNA ends (RACE) method...
2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27131730/chemical-properties-and-antioxidant-activity-of-a-water-soluble-polysaccharide-from-dendrobium-officinale
#12
Qiu-Lian Luo, Zhuan-Hui Tang, Xue-Feng Zhang, Yong-Hong Zhong, Su-Zhi Yao, Li-Sheng Wang, Cui-Wu Lin, Xuan Luo
In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography. The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da. Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6...
August 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27034693/dendrobium-officinale-prevents-early-complications-in-streptozotocin-induced-diabetic-rats
#13
Shao-Zhen Hou, Chu-Yan Liang, Hua-Zhen Liu, Dong-Mei Zhu, Ya-Yun Wu, Jian Liang, Ya Zhao, Jian-Ru Guo, Song Huang, Xiao-Ping Lai
Background. Dendrobium officinale (DO) Kimura et Migo is a precious Chinese herb that is considered beneficial for health due to its antioxidant and antidiabetes properties, and so on. In this research, we try to determine the preventive effect of DO on the early complications of STZ-induced diabetic rats. Methods. Type 1 diabetic rats were produced with a single intraperitoneal injection of STZ (50 mg/kg). DO (1 g/kg/day) was then orally administered for 5 weeks. Blood glucose, TC, TG, BUN, CREA, and GSH-PX levels were determined, and electroretinographic activity and hypoalgesia were investigated...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/26983198/-study-on-suitable-harvest-time-of-dendrobium-officinale-in-yunnan-province
#14
Shan-bao Zhang, Ke-jun Zhou, Zhen Zhang, Rui-rui Lu, Xian Li, Xiao-hua Li
In order to determine the suitable harvest time of Dendrobium officinale from different regions in Yunnan province, the drying rate, mannose and glucose peak area ratio, extract, contents of polysaccharide and mannose of D. officinale samples collected from six producing areas in Ynnnan province were determined. The results indicate that drying rate and the contents of polysaccharide and mannose arrived the peak from January to April, extract reached a higher content from September to December, and mannose and glucose peak area ratio from October to February of the coming met the requirment of the Chinese Pharmacopoeia...
September 2015: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/26904032/transcriptome-analysis-of-dendrobium-officinale-and-its-application-to-the-identification-of-genes-associated-with-polysaccharide-synthesis
#15
Jianxia Zhang, Chunmei He, Kunlin Wu, Jaime A Teixeira da Silva, Songjun Zeng, Xinhua Zhang, Zhenming Yu, Haoqiang Xia, Jun Duan
Dendrobium officinale is one of the most important Chinese medicinal herbs. Polysaccharides are one of the main active ingredients of D. officinale. To identify the genes that maybe related to polysaccharides synthesis, two cDNA libraries were prepared from juvenile and adult D. officinale, and were named Dendrobium-1 and Dendrobium-2, respectively. Illumina sequencing for Dendrobium-1 generated 102 million high quality reads that were assembled into 93,881 unigenes with an average sequence length of 790 base pairs...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/26858106/natpipe-an-integrative-pipeline-for-systematical-discovery-of-natural-antisense-transcripts-nats-and-phase-distributed-nat-sirnas-from-de-novo-assembled-transcriptomes
#16
Dongliang Yu, Yijun Meng, Ziwei Zuo, Jie Xue, Huizhong Wang
Nat-siRNAs (small interfering RNAs originated from natural antisense transcripts) are a class of functional small RNA (sRNA) species discovered in both plants and animals. These siRNAs are highly enriched within the annealed regions of the NAT (natural antisense transcript) pairs. To date, great research efforts have been taken for systematical identification of the NATs in various organisms. However, developing a freely available and easy-to-use program for NAT prediction is strongly demanded by researchers...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26697665/investigation-of-original-materials-of-chinese-medicine-shihu-and-tiepishihu
#17
Xiao-hua Jin, Lu-qi Huang
There are two types of Chinese medicine, i.e., "Shihu" and "Tiepi shihu", using the stems of species of Dendrobium (Orchidaceae) as original materials. In order to understand species of Dendrobium using in Chinese medicine, taxonomic investigation and revision were performed. Our results indicated that about sixty species of sect. Dendrobium, sect. Formosae, and Sect. Stachyobium, are used as raw materials of "Shihu". Seven species of Dendrobium moniliforme complex and four species in Dendrobium officinale complex were recognized...
July 2015: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/26592697/structural-characterization-and-immunomodulating-activity-of-polysaccharide-from-dendrobium-officinale
#18
Tao-Bin He, Yan-Ping Huang, Liu Yang, Ti-Ti Liu, Wan-Ying Gong, Xuan-Jun Wang, Jun Sheng, Jiang-Miao Hu
A neutral heteropolysaccharide (DOP-1-1) consisted by mannose and glucose (5.9:1) with an average molecular weight at about 1.78×10(5) Da was purified from Dendrobium officinale. Based on Fourier transform infrared spectrum (FT-IR) and nuclear magnetic resonance (NMR) spectra, it suggested that partial structure of DOP-1-1 is an O-acetylated glucomannan with β-d configuration in pyranose sugar forms. The immunomodulatory activity of DOP-1-1 was evaluated by secretion level of cytokine (interleukin (IL)-1β and IL-10) and tumor necrosis factor (TNF)-α in vitro...
February 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/26539215/similarity-evaluation-of-different-origins-and-species-of-dendrobiums-by-gc-ms-and-ftir-analysis-of-polysaccharides
#19
Nai-Dong Chen, Nai-Fu Chen, Jun Li, Cai-Yun Cao, Jin-Mei Wang, He-Ping Huang
GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species...
2015: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/26329540/study-on-dendrobium-officinale-o-acetyl-glucomannan-dendronan-7-improving-effects-on-colonic-health-of-mice
#20
Guan-ya Zhang, Shao-ping Nie, Xiao-jun Huang, Jie-lun Hu, Steve W Cui, Ming-yong Xie, Glyn O Phillips
This research was aimed to study the effect of Dendrobium officinale polysaccharide (Dendronan) on colonic health. Mice were fed Dendronan at doses of 40, 80, and 160 mg/kg body weight for 0, 10, 20, and 30 days, respectively. Results showed that Dendronan, which has a special structure formed by mannose and glucose, rich in O-acetyl groups, exhibited improving effects on colonic and fecal parameters of Balb/c mice. After Dendronan feeding, the content of short-chain fatty acids (SCFAs), colon length and index, and fecal moisture were increased, whereas colonic pH was decreased and defecation time was shortened...
March 30, 2016: Journal of Agricultural and Food Chemistry
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