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Dendrobium officinale

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https://www.readbyqxmd.com/read/28942180/de-novo-transcriptome-analysis-in-dendrobium-and-identification-of-critical-genes-associated-with-flowering
#1
Yue Chen, Qi Shen, Renan Lin, Zhuangliu Zhao, Chenjia Shen, Chongbo Sun
Artificial control of flowering time is pivotal for the ornamental value of orchids including the genus Dendrobium. Although various flowering pathways have been revealed in model plants, little information is available on the genetic regualtion of flowering in Dendrobium. To identify the critical genes associated with flowering, transcriptomes from four organs (leaf, root, stem and flower) of D. officinale were analyzed in our study. In total, 2645 flower-specific transcripts were identified. Functional annotation and classification suggested that several metabolic pathways, including four sugar-related pathways and two fatty acid-related pathways, were enriched...
September 14, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28914810/an-nmr-based-metabolomic-approach-to-unravel-the-preventive-effect-of-water-soluble-extract-from-dendrobium-officinale-kimura-migo-on-streptozotocin-induced-diabetes-in-mice
#2
Hong Zheng, Linlin Pan, Pengtao Xu, Jianjun Zhu, Ruohan Wang, Wenzong Zhu, Yongsheng Hu, Hongchang Gao
Dendrobium officinale Kimura & Migo (D. officinale) is a precious herbal medicine. In this study, we investigated metabolic mechanism underlying the effect of D. officinale water extract (DOWE) on diabetes prevention in mice after streptozotocin (STZ) exposure using NMR-based metabolomics. Interestingly, we found a decrease in blood glucose and an increase in liver glycogen in mice pretreated with DOWE after STZ exposure. The DOWE pretreatment significantly increased citrate and glutamine in the serum as well as creatine, alanine, leucine, isoleucine, valine, glutamine, glutathione and taurine in the liver of STZ-treated mice...
September 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28911623/a-comparative-study-of-three-tissue-cultured-dendrobium-species-and-their-wild-correspondences-by-headspace-gas-chromatography-mass-spectrometry-combined-with-chemometric-methods
#3
Nai-Dong Chen, Tao You, Jun Li, Li-Tao Bai, Jing-Wen Hao, Xiao-Yuan Xu
Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn...
October 2016: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28902405/preparation-and-characterization-of-dendrobium-officinale-powders-through-superfine-grinding
#4
Qingran Meng, Haoran Fan, Feng Chen, Tiancun Xiao, Lianfu Zhang
BACKGROUND: Dendrobium officinale has been used in China for several thousand years as a health food, it has become one of the most expensive tea materials worldwide due to extremely scarce wild resources and increasing demand. Hence, it is very important to improve the depth and width of its application. In present work, the physico-chemical, surface chemistry, and thermal properties of micron range particles and coarse particles prepared by superfine grinding and shear pulverization were investigated...
September 13, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28835632/a-genome-wide-identification-of-the-wrky-family-genes-and-a-survey-of-potential-wrky-target-genes-in-dendrobium-officinale
#5
Chunmei He, Jaime A Teixeira da Silva, Jianwen Tan, Jianxia Zhang, Xiaoping Pan, Mingzhi Li, Jianping Luo, Jun Duan
The WRKY family, one of the largest families of transcription factors, plays important roles in the regulation of various biological processes, including growth, development and stress responses in plants. In the present study, 63 DoWRKY genes were identified from the Dendrobium officinale genome. These were classified into groups I, II, III and a non-group, each with 14, 28, 10 and 11 members, respectively. ABA-responsive, sulfur-responsive and low temperature-responsive elements were identified in the 1-k upstream regulatory region of DoWRKY genes...
August 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28822173/-breakthrough-in-key-science-and-technologies-in-dendrobium-catenatum-industry
#6
Ji-Ping Si, Qi Wang, Zhong-Jian Liu, Jing-Jing Liu, Yi-Bo Luo
In view of the significant difficulties of propagation, planting and simple product in Dendrobium catenatum(D. officinale)industry development, a series of research were carried out. Genome study showed that D. catenatum is a specie of diploid with 38 chromosomes and 28 910 protein-coding genes. It was identified that specific genes accumulated in different organs at the transcriptome level. We got an insight into the gene regulation mechanism of the loss of the endospermous seed, the wide ecological adaptability and the synthesis of polysaccharides, which provided a theoretical basis for genetic engineering breeding and development and utilization of active pharmaceutical ingredients...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28797234/succinyl-proteome-profiling-of-dendrobium-officinale-an-important-traditional-chinese-orchid-herb-revealed-involvement-of-succinylation-in-the-glycolysis-pathway
#7
Shangguo Feng, Kaili Jiao, Hong Guo, Mengyi Jiang, Juan Hao, Huizhong Wang, Chenjia Shen
BACKGROUND: Lysine succinylation is a ubiquitous and important protein post-translational modification in various eukaryotic and prokaryotic cells. However, its functions in Dendrobium officinale, an important traditional Chinese orchid herb with high polysaccharide contents, are largely unknown. RESULTS: In our study, LC-MS/MS was used to identify the peptides that were enriched by immune-purification with a high-efficiency succinyl-lysine antibody. In total, 314 lysine succinylation sites in 207 proteins were identified...
August 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28769988/chemical-differentiation-of-dendrobium-officinale-and-dendrobium-devonianum-by-using-hplc-fingerprints-hplc-esi-ms-and-hptlc-analyses
#8
Zi Ye, Jia-Rong Dai, Cheng-Gang Zhang, Ye Lu, Lei-Lei Wu, Amy G W Gong, Hong Xu, Karl W K Tsim, Zheng-Tao Wang
The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28755575/protective-effect-of-dendrobium-officinale-polysaccharides-on-h2o2-induced-injury-in-h9c2-cardiomyocytes
#9
Xiaoyan Zhao, Mengmeng Dou, Zhihao Zhang, Duoduo Zhang, Chengzhi Huang
The preliminary studies have shown that Dendrobium officinale possessed therapeutic effects on hypertension and atherosclerosis. Studies also reported that Dendrobium officinale polysaccharides showed antioxidant capabilities. However, little is known about its effects on myocardial cells under oxidative stress. The present study was designed to study the protective effect of Dendrobium officinale polysaccharides against H2O2-induced oxidative stress in H9c2 cells. MTT assay was carried out to determine the cell viability of H9c2 cells when pretreated with Dendrobium officinale polysaccharides...
October 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28736679/chloroplast-genome-sequence-annotation-of-i-dendrobium-nobile-i-asparagales-orchidaceae-an-endangered-medicinal-orchid-from-northeast-india
#10
Devendra Biswal, Ruchishree Konhar, Manish Debnath, Sriram Parameswaran, Durai Sundar, Pramod Tandon
Orchidaceae constitutes one of the largest families of angiosperms. Owing to the significance of orchids in plant biology, market needs and current sustainable technology levels, basic research on the biology of orchids and their applications in the orchid industry is increasing. Although chloroplast (cp) genomes continue to be evolutionarily informative, there is very limited information available on orchid chloroplast genomes in public repositories. Here, we report the complete cp genome sequence of Dendrobium nobile from Northeast India (Orchidaceae, Asparagales), bearing the GenBank accession number KX377961, which will provide valuable information for future research on orchid genomics and evolution, as well as the medicinal value of orchids...
May 19, 2017: PLoS Currents
https://www.readbyqxmd.com/read/28684298/polysaccharides-of-dendrobium-officinale-kimura-migo-protect-gastric-mucosal-cell-against-oxidative-damage-induced-apoptosis-in-vitro-and-in-vivo
#11
Qiang Zeng, Chun-Hay Ko, Wing-Sum Siu, Long-Fei Li, Xiao-Qiang Han, Liu Yang, Clara Bik-San Lau, Jiang-Miao Hu, Ping-Chung Leung
ETHNOPHARMACOLOGICAL RELEVANCE: Dendrobium officinale Kimura & Migo (DO) is a valuable Traditional Chinese Medicine to nourish stomach, in which polysaccharides are identified as active ingredients. However, limited scientific evidences have been reported on the gastroprotective efficacy of DO. The aim of the current study was to investigate the protective effects and underlying mechanism of polysaccharides from DO(DOP) on gastric mucosal injury. MATERIAL AND METHODS: For in vitro study, HFE145 cells were pretreated with DOP before induction of cell apoptosis by H2O2...
July 4, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28631817/application-of-high-resolution-melting-analysis-for-authenticity-testing-of-valuable-dendrobium-commercial-products
#12
Xiaoman Dong, Chao Jiang, Yuan Yuan, Daiyin Peng, Yuqin Luo, Yuyang Zhao, Luqi Huang
BACKGROUND: The accurate identification of botanical origin in commercial products is important to ensure food authenticity and safety for consumers. The Dendrobium species have long been commercialised as functional food supplements and herbal medicines in Asia. Three valuable Dendrobium species, namely Dendrobium officinale, D. huoshanense and D. moniliforme, are often mutually adulterated in trade products in pursuit of higher profit. RESULTS: In this paper, a rapid and reliable semi-quantitative method for identifying the botanical origin of Dendrobium products in terminal markets was developed using high-resolution melting (HRM) analysis with specific primer pairs to target the trnL-F region...
June 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28587963/a-polysaccharide-of-dendrobium-officinale-ameliorates-h2o2-induced-apoptosis-in-h9c2-cardiomyocytes-via-pi3k-akt-and-mapk-pathways
#13
Jing-Yi Zhang, Ying Guo, Jin-Ping Si, Xiao-Bo Sun, Gui-Bo Sun, Jing-Jing Liu
Dendrobium officinale is one valuable traditional Chinese medicine, which has skyscraping medicinal value. Polysaccharide is the main active ingredient in D. officinale; its antioxidant activity is a hot research topic nowadays. Oxidative stress plays an important role in the pathological progress of a variety of cardiovascular disease, as one of key factors of cardiomyocyte apoptosis. This research adopts a model of H2O2 induction-H9c2 cardiomyocytes apoptosis, aiming to study the effect of Dendrobium officinale Polysaccharide (DOP-GY) for cardiomyocyte apoptosis caused by oxidative stress and its possible mechanism...
November 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28528944/structure-analysis-of-a-novel-heteroxylan-from-the-stem-of-dendrobium-officinale-and-anti-angiogenesis-activities-of-its-sulfated-derivative
#14
Han Yue, Yanqiu Liu, Huanhuan Qu, Kan Ding
Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on. Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated. In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide. It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8...
October 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28488443/itraq-and-rna-seq-analyses-provide-new-insights-into-regulation-mechanism-of-symbiotic-germination-of-dendrobium-officinale-seeds-orchidaceae
#15
Juan Chen, Si Si Liu, Annegret Kohler, Bo Yan, Hong Mei Luo, Xiao Mei Chen, Shun Xing Guo
Mycorrhizal fungi colonize orchid seeds and induce germination. This so-called symbiotic germination is a critical developmental process in the lifecycle of all orchid species. However, the molecular changes that occur during orchid seed symbiotic germination remain largely unknown. To better understand the molecular mechanism of orchid seed germination, we performed a comparative transcriptomic and proteomic analysis of the Chinese traditional medicinal orchid Dendrobium officinale to explore the change in protein expression at the different developmental stages during asymbiotic and symbiotic germination and identify the key proteins that regulate the symbiotic germination of orchid seeds...
May 17, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28471373/identification-and-expression-profiling-of-the-auxin-response-factors-in-dendrobium-officinale-under-abiotic-stresses
#16
Zhehao Chen, Ye Yuan, Di Fu, Chenjia Shen, Yanjun Yang
Auxin response factor (ARF) proteins play roles in plant responses to diverse environmental stresses by binding specifically to the auxin response element in the promoters of target genes. Using our latest public Dendrobium transcriptomes, a comprehensive characterization and analysis of 14 DnARF genes were performed. Three selected DnARFs, including DnARF1, DnARF4, and DnARF6, were confirmed to be nuclear proteins according to their transient expression in epidermal cells of Nicotiana benthamiana leaves. Furthermore, the transcription activation abilities of DnARF1, DnARF4, and DnARF6 were tested in a yeast system...
May 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28446922/corrigendum-building-a-genetic-manipulation-tool-box-for-orchid-biology-identification-of-constitutive-promoters-and-application-of-crispr-cas9-in-the-orchid-dendrobium-officinale
#17
Ling Kui, Haitao Chen, Weixiong Zhang, Simei He, Zijun Xiong, Yesheng Zhang, Liang Yan, Chaofang Zhong, Fengmei He, Junwen Chen, Peng Zeng, Guanghui Zhang, Shengchao Yang, Yang Dong, Wen Wang, Jing Cai
[This corrects the article on p. 2036 in vol. 7, PMID: 28127299.].
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28386292/dendrobium-officinale-kimura-et-migo-a-review-on-its-ethnopharmacology-phytochemistry-pharmacology-and-industrialization
#18
REVIEW
Hanxiao Tang, Tianwen Zhao, Yunjie Sheng, Ting Zheng, Lingzhu Fu, Yongsheng Zhang
Ethnopharmacological Relevance. Dendrobii Officinalis Caulis, the stems of Dendrobium officinale Kimura et Migo, as a tonic herb in Chinese materia medica and health food in folk, has been utilized for the treatment of yin-deficiency diseases for decades. Methods. Information for analysis of Dendrobium officinale Kimura et Migo was obtained from libraries and Internet scientific databases such as PubMed, Web of Science, Google Scholar, ScienceDirect, Wiley InterScience, Ingenta, Embase, CNKI, and PubChem. Results...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28304124/distinguishing-the-chinese-materia-medica-tiepishihu-from-similar-dendrobium-species-of-the-same-genus-using-histological-and-microscopic-method
#19
Kun-Zi Yu, Hua Yan, Hai-Chuan Tai, Nan-Ping Zhang, Xian-Long Cheng, Zeng-Xi Guo, Shuang-Cheng Ma, Feng Wei
The Chinese Materia Medica, Tiepishihu, used as a tonic for over one thousand years, is a well-known precious medicine in China. According to the Chinese Pharmacopoeia, its source is the species Dendrobium officinale Kimura et Migo, which is distinguished from other species in Dendrobium genus. However, these species from the same genus are similar with Tiepishihu and caused confusion in the market. To find a quick and simple method to distinguish Tiepishihu from other similar species, histologic and microscopic methods were combined together to investigate the transverse section of stem of Tiepishihu and other similar species...
March 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28298629/identification-and-analysis-of-genes-associated-with-the-synthesis-of-bioactive-constituents-in-dendrobium-officinale-using-rna-seq
#20
Chenjia Shen, Hong Guo, Hailing Chen, Yujun Shi, Yijun Meng, Jiangjie Lu, Shangguo Feng, Huizhong Wang
Dendrobium officinale L. is an important traditional herb with high commercial value in China. Several bioactive constituents, including polysaccharides and alkaloids, reportedly make major contributions toward the excellent medicinal effect of D. officinale. In this study, the contents of polysaccharides and alkaloids in various organs of D. officinale were measured and compared. We took advantage of transcriptomes from four organs to explore biological mechanisms in the organ-specific distribution of active ingredients in D...
March 15, 2017: Scientific Reports
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