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Dendrobium officinale

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https://www.readbyqxmd.com/read/29762539/study-on-the-polar-extracts-of-dendrobium-nobile-d-officinale-d-loddigesii-and-flickingeria-fimbriata-metabolite-identification-content-evaluation-and-bioactivity-assay
#1
Huiping Chen, Xuewen Li, Yongli Xu, Kakei Lo, Huizhen Zheng, Haiyan Hu, Jun Wang, Yongcheng Lin
The polar extract of the Dendrobium species or F. fimbriata (a substitute of Dendrobium ), between the fat-soluble extract and polysaccharide has barely been researched. This report worked on the qualitative and quantitative studies of polar extracts from D. nobile , D. officinale , D. loddigesii , and F. fimbriata . Eight water-soluble metabolites containing a new diglucoside, flifimdioside A ( 1 ), and a rare imidazolium-type alkaloid, anosmine ( 4 ), were identified using chromatography as well as spectroscopic techniques...
May 15, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29726707/extraction-purification-and-antioxidant-activity-of-novel-polysaccharides-from-dendrobium-officinale-by-deep-eutectic-solvents
#2
Jing Liang, Yingjie Zeng, Hongfeng Wang, Wenyong Lou
Two novel polysaccharides DOP1-DES and DOP2-DES with molecular weight of 297,911 Da and 30,400 Da were extracted by using DESs from Dendrobium officinale for the first time, and purified with macroporous resin AB-8, Sephadex G75 and Sephadex G200 chromatography. Besides, the effect of extraction temperature, molar ratio of DES, reaction time and compound enzymes concentration on the extraction yield of polysaccharides were investigated. Monosaccharide composition analysis showed DOP1-DES and DOP2-DES were composed of D-glucose and D-mannose with the ratio of 2...
May 4, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29676118/-isolation-and-expression-analysis-of-a-hexose-transporter-gene-doht1-in-dendrobium-officinale
#3
Yi-Min Li, Ying Chen, A-Ping Liu, Na Zhang, Xiao-Bin Hei, Gang Zhang, Shun-Xing Guo
In this study, RACE technology was employed to isolate the full length cDNA of DoHT1 in Dendrobium officinale, followed by bioinformatics analysis of the sequence characteristics. And the expression pattern of the gene was also analyzed by quantitative PCR. The full length cDNA of DoHT1 was 1 586 bp in length, containing a 1 536 bp ORF, which encoded a 511-aa protein with molecular weight of 56.18 kD and isoelectric point of 9.08. The deduced DoHT1 protein had the major facilitator superfamily conserved domain (22-483), SUGAR₋TRANSPORT₋1 (139-164), and SUGAR₋TRANSPORT₋2 (338-355), typical for sugar transporter; DoHT1, without a single peptide had 11 transmembrane regions, and was predicted to locate in the plasma membrane; DoHT1 had high identities (54...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676117/-geographical-origin-discriminant-of-dendrobium-officinale-based-on-stable-isotope-ratios
#4
Jian Yang, Hao Wu, Chao-Geng Lv, Sheng Wang, Xiao-Bo Zhang, Guang Yang, Lan-Ping Guo, Lu-Qi Huang
The isotopic ratios of strontium isotope (Sr) and light elements (C/H/O/N) in Dendrobium officinale from different producing areas (Shaoguan, Guangdong; Yulin, Guangxi; Shibing, Guizhou; Wenshan, Yunnan and Zhejiang province) were determined with thermal ionization mass spectrometry (TI-MS) and isotope ratio mass spectrometry (IRMS).The differences of the stable isotope ratios in D. officinale were obtained by the variance analysis and the correlation analysis, and pattern recognition techniques with principal component analysis (PCA) was used to classify the geographical origins of D...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29651680/simultaneous-identification-and-determination-of-flavonoids-in-dendrobium-officinale
#5
Chunhua Zhou, Zhenshan Xie, Zhouxi Lei, Yuechun Huang, Gang Wei
BACKGROUND: The quality of material medicine resources has had a considerable impact on the development of the health industry, which has created a bottleneck for traditional Chinese medicine (TCM). Dendrobium officinale, which has been widely used for health prevention in TCM, has become a high-nutritive health food that is strongly recommended by many white-collar workers and people who pay more attention to their health. The aim of this study was to develop a method to authenticate and evaluate D...
April 12, 2018: Chemistry Central Journal
https://www.readbyqxmd.com/read/29614010/extraction-and-analysis-of-gigantol-from-dendrobium-officinale-with-response-surface-methodology
#6
Siyan Zheng, Yingpeng Zhu, Chunyan Jiao, Mengyao Shi, Lianping Wei, Yang Zhou, Qing Jin, Yongping Cai
In order to optimize the extraction of gigantol from Dendrobium officinale , the influence of methanol concentration, ultrasonic temperature, and liquid ratio on extraction efficiency was analysed by the response surface analysis method. The results show that the extraction rate reached a maximum when the methanol concentration was 92.98%, the solid-liquid ratio was 27.2 mL/g, and the extraction temperature was 41.41 °C. The content of gigantol of Dendrobium officinale in leaves was significantly higher than that in stems, reaching 4...
April 3, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29581723/optimum-extraction-characterization-and-antioxidant-activities-of-polysaccharides-from-flowers-of-dendrobium-devonianum
#7
Donghui Wang, Bei Fan, Yan Wang, Lijing Zhang, Fengzhong Wang
Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum . The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g. Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method...
2018: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/29555359/characterization-of-plant-polysaccharides-from-dendrobium-officinale-by-multiple-chromatographic-and-mass-spectrometric-techniques
#8
Huiying Ma, Keke Zhang, Qing Jiang, Diya Dai, Hongli Li, Wentao Bi, David Da Yong Chen
Plant polysaccharides have numerous medicinal functions. Due to the differences in their origins, regions of production, and cultivation conditions, the quality and the functions of polysaccharides can vary significantly. They are macromolecules with large molecular weight (MW) and complex structure, and pose great challenge for the analytical technology used. Taking Dendrobium officinale (DO) from various origins and locations as model samples. In this investigation, mechanochemical extraction method was used to successfully extract polysaccharides from DO using water as solvent, the process is simple, fast (40 s) and with high yield...
March 13, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29552825/-antihypertensive-effect-and-mechanism-of-dendrobium-officinale-flos-on-high-blood-pressure-rats-induced-by-high-glucose-and-high-fat-compound-alcohol
#9
Kai-Lun Liang, Ping Fang, Qiu-Qiu Shi, Jie Su, Bo Li, Su-Hong Chen, Gui-Yuan Lv
This study aimed to investigate the antihypertensive effect and possible mechanism of Dendrobium officinale flos on hypertensive rats induced by high glucose and high fat compound alcohol. The hypertensive models were successfully made by high-glucose and high-fat diet, with gradient drinking for 4 weeks, and then divided into model control group, valsartan (5.7 mg·kg⁻¹) positive control group and D. officinale flos groups (3,1 g·kg⁻¹). After 6 weeks of treatment, the blood pressure of rats was measured regularly...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29510516/metabolic-profiling-of-dendrobium-officinale-in-response-to-precursors-and-methyl-jasmonate
#10
Chunyan Jiao, Cheng Song, Siyan Zheng, Yingpeng Zhu, Qing Jin, Yongping Cai, Yi Lin
Alkaloids are the main active ingredients in the medicinal plant Dendrobium officinale . Based on the published genomic and transcriptomic data, a proposed terpenoid indole alkaloid (TIA) biosynthesis pathway may be present in D. officinale . In this study, protocorm-like bodies (PLBs) with a high-yielding production of alkaloids were obtained by the optimization of tryptophan, secologanin and methyl jasmonate (MeJA) treatment. The results showed that the total alkaloid content was 2.05 times greater than that of the control group when the PLBs were fed with 9 µM tryptophan, 6 µM secologanin and 100 µM MeJA after 36 days...
March 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29421053/therapeutic-roles-of-polysaccharides-from-dendrobium-officinaleon-colitis-and-its-underlying-mechanisms
#11
Jian Liang, Shuxian Chen, Jianhui Chen, Jizong Lin, Qingping Xiong, Yiqi Yang, Jun Yuan, Lian Zhou, Lian He, Shaozhen Hou, Shijie Li, Song Huang, Xiaoping Lai
Polysaccharide, as a promising candidate to meet the medication requirement of ulcerative colitis (UC), is increasingly attracting extensive interest. Dendrobium officinale has been widely used to treat gastrointestinal sickness in the clinical treatment of Traditional Chinese Medicine. However, it remains largely unknown whether polysaccharides (DOPS) from Dendrobium officinale can treat UC. The purpose of this paper is to confirm therapeutic action of DOPS to UC and explored its underlying mechanisms. We noted that DOPS could dramatically improve clinical signs and symptoms, decrease mortality, alleviate colonic pathological damage, and reestablish the balance of pro- and anti-inflammatory cytokines in DSS-induced acute UC mice...
April 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29243428/-relative-molecular-mass-and-monosaccharide-composition-of-polysaccharide-in-dendrobium-officinale-flowers
#12
Si-Jie Zhang, Zheng Qian, Jing-Jing Liu, Xin-Feng Zhang, Jin-Ping Si
In order to provide new information on polysaccharide in Dendrobium officinale flowers,the monosaccharide composition and relative molecular mass distribution of 11 families of flowers were investigated and analyzed by high performance gel filtration chromatography (HPGFC) and pre-column derivatization ultra performance liquid chromatography (UPLC) in this study. Then cluster analysis was carried out for the monosaccharide peak areas by utilizing SPSS 19.0 software. The results showed that the polysaccharides of all the above 11 hybrid families of D...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29234904/post-genomics-era-for-orchid-research
#13
REVIEW
Wen-Chieh Tsai, Anne Dievart, Chia-Chi Hsu, Yu-Yun Hsiao, Shang-Yi Chiou, Hsin Huang, Hong-Hwa Chen
Among 300,000 species in angiosperms, Orchidaceae containing 30,000 species is one of the largest families. Almost every habitats on earth have orchid plants successfully colonized, and it indicates that orchids are among the plants with significant ecological and evolutionary importance. So far, four orchid genomes have been sequenced, including Phalaenopsis equestris, Dendrobium catenatum, Dendrobium officinale, and Apostaceae shengen. Here, we review the current progress and the direction of orchid research in the post genomics era...
December 12, 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/29090564/-herbal-textual-research-on-relationship-between-chinese-medicine-shihu-dendrobium-spp-and-tiepi-shihu-d-catenatum
#14
Jin-Ping Si, Yuan Zhang, Yi-Bo Luo, Jing-Jing Liu, Zhong-Jian Liu
Dendrobium species on the ancient Chinese herbal texts were investigated in this paper, including their dscriptions of original species, producing areas and quality. Our results indicated that the major producing areas were Lu'an, Anhui province and Wenzhou, Taizhou, Zhejiang province. In addition, the sweet flavor, short, thin and solid stems were standing for good quality. Based on the stable producing areas and quality descriptions, D. catenatum (D. officinale) ("Tiepi Shihu") and D. houshanense were high quality medicinal Dendrobium species ("Shihu" ) in ancient China...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29090555/-protective-effects-of-five-different-types-of-dendrobium-on-ccl4-induced-liver-injury-in-mice
#15
Kai Wang, Dan-Juan Sui, Chang-Suo Wang, Li Yang, Zhen Ouyang, Nai-Fu Chen, Bang-Xing Han, Yuan Wei
This study aims to investigate the protective effect of Dendrobium huoshanense, D.officinale(Huoshan), D.officinale(Yunnan), D.moniliforme and D. henanense on CCl4-induced hepatic damage in mice. C57BL/6 mice were randomly divided into control group, model group, high-dose(7.5 g•kg⁻¹) and low-dose (1.25 g•kg⁻¹) groups of the five Dendrobium. Each group was intragastrically administered with drugs for 2 weeks. The control group was intraperitoneally injected with Olive oil solution, while the other groups were intraperitoneally given 0...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29074081/protective-roles-and-mechanisms-of-dendrobium-officinal-polysaccharides-on-secondary-liver-injury-in-acute-colitis
#16
Jian Liang, Shuxian Chen, Youdong Hu, Yiqi Yang, Jun Yuan, Yanfang Wu, Shijie Li, Jizhong Lin, Lian He, Shaozhen Hou, Lian Zhou, Song Huang
The purpose of this study was to investigate the protective roles and mechanisms of Dendrobium officinale polysaccharides (DOPS) on secondary liver injury in acute colitis. Firstly, the mice model of secondary liver injury in acute colitis was induced by 4% Dextran sodium sulfate (DSS). Inflammatory cell model was established by LPS-stimulated RAW264.7 cells. Then, the protective roles of DOPS were evaluated by both in vivo and in vitro experiment. The results showed that DOPS attenuated DSS-induced hepatic pathological damage, liver parameters, infiltration of macrophages, cytokines levels, MDA level and increased the antioxidant enzymes activities...
February 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29063360/new-phenanthrene-and-9-10-dihydrophenanthrene-derivatives-from-the-stems-of-dendrobium-officinale-with-their-cytotoxic-activities
#17
Gui-Yun Zhao, Bo-Wen Deng, Chong-Yu Zhang, Yi-Da Cui, Jia-Yi Bi, Guo-Gang Zhang
Two new phenanthrene and 9, 10-dihydrophenanthrene derivatives (1-2) with six known congeners (3-8) were isolated from the extraction of stems of Dendrobium officinale. Compounds 1 and 2 were based on carbon skeleton in which phenanthrene and 9, 10-dihydrophenanthrene moiety were linked with a phenylpropane unit through a dioxane bridge, respectively. Their structures were determined by comprehensive NMR spectroscopic data, the absolute configuration of new compounds were determined by comparing their experimental and calculated ECD for the first time...
January 2018: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28994532/-study-on-identification-of-dendrobium-officinale-and-related-species-by-bidirectional-pcr-amplification-of-mismatched-and-specific-alleles
#18
Xiao-Man Dong, Chao Jiang, Yuan Yuan, Liang-Ping Zha, Dai-Yin Peng, Yu-Yang Zhao
Based on rDNA ITS sequences of Dendrobium officinale and the other 69 species of Dendrobium, a pair of dismatched allele-specific diagnostic primers, TPSH-AS1F and TPSH-AS1R were designed to authenticate D. officinale from the other species. Thebidirectional PCR amplification were performed using the diagnostic primers with the total DNAs of the original plants or processing products as a template. When the annealing temperature was raised to 60 ℃, only the template DNA of D. officinale could be amplified whereas the diagnostic PCRs of the other Dendrobium species were all negative...
March 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28981454/hybrid-sequencing-of-full-length-cdna-transcripts-of-stems-and-leaves-in-dendrobium-officinale
#19
Liu He, Shuhua Fu, Zhichao Xu, Jun Yan, Jiang Xu, Hong Zhou, Jianguo Zhou, Xinlian Chen, Ying Li, Kin Fai Au, Hui Yao
Dendrobium officinale is an extremely valuable orchid used in traditional Chinese medicine, so sought after that it has a higher market value than gold. Although the expression profiles of some genes involved in the polysaccharide synthesis have previously been investigated, little research has been carried out on their alternatively spliced isoforms in D. officinale. In addition, information regarding the translocation of sugars from leaves to stems in D. officinale also remains limited. We analyzed the polysaccharide content of D...
October 5, 2017: Genes
https://www.readbyqxmd.com/read/28942180/de-novo-transcriptome-analysis-in-dendrobium-and-identification-of-critical-genes-associated-with-flowering
#20
Yue Chen, Qi Shen, Renan Lin, Zhuangliu Zhao, Chenjia Shen, Chongbo Sun
Artificial control of flowering time is pivotal for the ornamental value of orchids including the genus Dendrobium. Although various flowering pathways have been revealed in model plants, little information is available on the genetic regualtion of flowering in Dendrobium. To identify the critical genes associated with flowering, transcriptomes from four organs (leaf, root, stem and flower) of D. officinale were analyzed in our study. In total, 2645 flower-specific transcripts were identified. Functional annotation and classification suggested that several metabolic pathways, including four sugar-related pathways and two fatty acid-related pathways, were enriched...
October 2017: Plant Physiology and Biochemistry: PPB
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