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https://www.readbyqxmd.com/read/28650796/comparison-of-bovine-bone-autogenic-bone-mixture-versus-platelet-rich-fibrin-for-maxillary-sinus-grafting-histologic-and-histomorphologic-study
#1
Hakan Ocak, Nukhet Kutuk, Umut Demetoglu, Esra Balcıoglu, Saim Ozdamar, Alper Alkan
Numerous grafting materials have been used to augment the maxillary sinus floor for long-term stability and success for implant-supported prosthesis. To enhance bone formation, adjunctive blood-born growth factor sources have gained popularity during the recent years. The present study compared the use of platelet-rich fibrin (PRF) and bovine-autogenous bone mixture for maxillary sinus floor elevation. A split-face model was used to apply 2 different filling materials for maxillary sinus floor elevation in 22 healthy adult sheep...
June 2017: Journal of Oral Implantology
https://www.readbyqxmd.com/read/28650694/investigation-of-the-developmental-potential-and-developmental-kinetics-of-bovine-parthenogenetic-and-somatic-cell-nuclear-transfer-embryos-using-a-time-lapse-monitoring-system
#2
Min-Jee Park, Eun-Young Kim, Man-Jong Kang, Jun-Beom Lee, Chang-Jin Jeong, Se-Pill Park
A time-lapse monitoring system has predictive value for selecting good-quality embryos with the highest implantation potential. Using this new tool, we investigated the developmental potential and developmental kinetics of bovine parthenogenetic (PA) and two types of somatic cell nuclear transfer (NT) embryos. Bovine non-transgenic ear cells (bECs) or transgenic cells (bTGCs) were used as donor cells. The cleavage and blastocyst development rates did not significantly differ among the PA, NT-bEC, and NT-bTGC groups, and first cleavage occurred an average of 19...
June 26, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28650693/healthy-bovines-as-reservoirs-of-major-pathogenic-lineages-of-staphylococcus-aureus-in-portugal
#3
Teresa Conceição, Hermínia de Lencastre, Marta Aires-de-Sousa
The nosocomial prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Portugal is close to 50% and remains one of the highest in Europe. MRSA reservoirs in the animal setting in Portugal have been very poorly investigated, namely among animal husbandry. A total of 52 samples (nasal, inguinal region, and milk) were obtained from bovine animals and analyzed for the presence of S. aureus. The isolates were characterized by pulsed-field gel electrophoresis (PFGE), spa typing, SCCmec typing, and multilocus sequence typing and tested for antimicrobial susceptibility, presence of mecA and mecC genes, and virulence determinants...
June 26, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28649896/platelet-lysate-as-a-serum-replacement-for-skin-cell-culture-on-biomimetic-pcl-nanofibers
#4
Vera Sovkova, Karolina Vocetkova, Michala Rampichova, Andrea Mickova, Matej Buzgo, Vera Lukasova, Jana Dankova, Eva Filova, Alois Necas, Evzen Amler
Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells (fibroblasts, melanocytes, and keratinocytes) were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added...
June 26, 2017: Platelets
https://www.readbyqxmd.com/read/28649801/vaccinia-virus-detection-in-dairy-products-made-with-milk-from-experimentally-infected-cows
#5
T M L de Oliveira, M I M C Guedes, I S Rehfeld, A C D Matos, A V Rivetti Júnior, A F da Cunha, M M O P Cerqueira, J S Abrahão, Z I P Lobato
Vaccinia virus (VACV) is the agent of bovine vaccinia (BV), an emerging zoonosis that causes exanthematic lesions on the teats of dairy cows and on the hands of milkers. The virus has been detected in the milk of naturally infected cows. The objective of this study was to investigate and quantify VACV DNA as well as the presence of infectious virus particles in samples of cheese curd, cheese whey and pasteurized milk produced using milk from cows experimentally inoculated with VACV-GP2, a Brazilian isolate of VACV (VACV-BR)...
June 26, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28649731/protein-glycan-quinary-interactions-in-crowding-environment-unveiled-by-nmr-spectroscopy
#6
Ana Diniz, Jorge S Dias, Jesús Jiménez-Barbero, Filipa Marcelo, Eurico J Cabrita
Protein-glycan interactions as modulators for quinary structure in crowding environments were explored. The interaction between human galectin 3 (Gal-3) and distinct macromolecular crowders, such as bovine and human serum albumin (BSA and HSA), Ficoll 70 and PEG3350, was scrutinized. The molecular recognition event of the specific ligand, lactose, by Gal-3 in crowding conditions was evaluated. Gal-3 interactions were monitored by NMR analysing chemical shift perturbation (CSP) and line broadening of 1H15N-HSQC signals...
June 25, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28649382/does-sperm-quality-and-dna-integrity-differ-in-cryopreserved-semen-samples-from-young-adult-and-aged-nellore-bulls
#7
J T Carreira, J T Trevizan, I R Carvalho, B Kipper, L H Rodrigues, C Silva, S H V Perri, J R Drevet, M B Koivisto
BACKGROUND: In humans, it is now well documented that rising paternal age is correlated with decreased sperm DNA integrity and embryonic developmental failures. On the other side of the coin, it is also reported that very young fathers such as teenagers carry an increased risk of adverse birth outcomes. These observations suggest that, at least in humans, there is an age window for optimal sperm DNA integrity. In bovine, little is known about sperm DNA quality in young bulls and how it evolves with age...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28649069/altered-antibacterial-activity-of-curcumin-in-the-presence-of-serum-albumin-plasma-and-whole-blood
#8
Sin Yeang Teow, Syed A Ali
Antibacterial effect is one of the major therapeutic activities of plant-derived Curcumin. This work evaluated the effect of serum albumin, human plasma, and whole blood on the in vitro activity of Curcumin against eight clinical bacterial isolates by standard broth microdilution and plate-counting methods. Toxicological effects of Curcumin towards human red blood cells (RBCs) and peripheral blood mononuclear cells (PBMCs) were also investigated. Curcumin exhibited weak activity against gram-negative bacteria, except Escherichia coli and Shigella flexneri were susceptible and was most active against gram-positive bacteria: Staphylococcus aureus, Streptococcus pyogenes and Enterococcus faecalis...
March 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28648864/impact-of-plasma-protein-binding-on-cargo-release-by-thermosensitive-liposomes-probed-by-fluorescence-correlation-spectroscopy
#9
Judith J Mittag, Barbara Kneidl, Tobias Preiß, Martin Hossann, Gerhard Winter, Stefan Wuttke, Hanna Engelke, Joachim O Rädler
Thermosensitive liposomes (TSLs) whose phase-transition temperature (Tm) lies slightly above body temperature are ideal candidates for controlled drug release via local hyperthermia. Recent studies, however, have revealed disruptive shifts in the release temperature Tr in mouse plasma, which are attributed to undefined interactions with blood proteins. Here, we study the effects of four major plasma proteins - serum albumin (SA), transferrin (Tf), apolipoprotein A1 (ApoA1) and fibrinogen (Fib) - on the temperature-dependent release of fluorescein di-β-D-galactopyranoside (FDG) from TSLs...
June 22, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28648453/tolerance-of-pseudomonas-aeruginosa-in-in-vitro-biofilms-to-high-level-peracetic-acid-disinfection
#10
Ayorinde Akinbobola, Leighann Sherry, William G Mckay, Gordon Ramage, Craig Williams
BACKGROUND: Biofilm has been suggested as a cause of disinfection failures in flexible endoscopes where no lapses in the decontamination procedure can be identified. To test this theory, the activity of peracetic acid (PAA), one of the commonly used disinfectants in the reprocessing of flexible endoscopes, was evaluated against both planktonic and sessile communities of Pseudomonas aeruginosa. AIM: To investigate the ability of P. aeruginosa biofilm to survive high level PAA disinfection...
June 22, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28648041/-effects-of-hypoxia-on-the-phenotype-transformation-of-human-dermal-fibroblasts-to-myofibroblasts-and-the-mechanism
#11
B Zhao, F Han, W Zhang, X J Wang, J Zhang, F F Yang, J H Shi, L L Su, D H Hu
Objective: To investigate the effects of hypoxia on the phenotype transformation of human dermal fibroblasts to myofibroblasts and the mechanism. Methods: The third passage of healthy adult human dermal fibroblasts in logarithmic phase were cultured in DMEM medium containing 10% fetal bovine serum for the following five experiments. (1) In experiments 1, 2, and 3, cells were divided into normoxia group and hypoxia group according to the random number table, with 10 dishes in each group. Cells of normoxia group were cultured in incubator containing 21% oxygen, while those of hypoxia group with 1% oxygen...
June 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28648030/-effect-and-mechanism-of-intermittent-alkaline-stimulation-on-high-phosphorus-induced-calcification-in-vascular-smooth-muscle-cells-of-rats
#12
Y L Bai, J S Xu, T Tian, J X Zhang, L W Cui, H R Zhang, S L Zhang
Objective: To explore the effect and possible mechanisms of intermittent alkaline on rat vascular smooth muscle cells (VSMCs) calcification induced by high phosphorus. Methods: VSMCs were isolated from rat thoracic aorta and cultured in vitro. The fourth generation VSMCs were randomly divided into control group, high phosphorus+ pH7.4, high phosphorus+ pH7.5, high phosphorus+ pH7.6 and high phosphorus+ pH7.7 group with random number table. The control group was cultured in DMEM with 10% fetal bovine serum. Other groups were cultured in DMEM with 10 mmol/L β-glycerophosphate and alkalized by 7...
June 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28647637/transcervical-carotid-artery-stenting-using-a-prosthetic-arterial-conduit-case-series-of-a-novel-surgical-technique
#13
Nandakumar Menon, Ali Khalifeh, Charles B Drucker, Suny Sahajwani, Danon Garrido, Richa Kalsi, Brajesh K Lal, Shahab Toursavadkohi
We present a series of 4 patients with carotid restenosis following carotid endarterectomy (CEA) who underwent transcervical carotid artery stenting (CAS) using a novel prosthetic conduit technique. The patients were high risk for repeat CEA (short and obese necks) and had contraindications to transfemoral CAS (Bovine arch, prior dissection). Carotid artery stenting was thus performed via a transcervical approach with a polytetrafluoroethylene (PTFE) conduit anastomosed to the proximal common carotid artery (CCA)...
June 21, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28647625/thermoresponsive-poly-glycidyl-ether-brushes-on-gold-surface-engineering-parameters-and-their-implication-for-cell-sheet-fabrication
#14
Silke Heinen, José Luis Cuellar-Camacho, Marie Weinhart
Thermoresponsive polymer coatings, optimized for cell adhesion and thermally-triggered cell detachment, allow the fabrication of confluent cell sheets with intact extracellular matrix. However, rational design guidelines for such coatings are rare, since temperature-triggered cell adhesion and detachment from thermoresponsive surfaces are mechanistically not well understood. Herein, we investigated the impact of molecular weight (2, 9, 24 kDa), grafting density (0.04 - 1.4 chains nm(-2)), morphology, and roughness of well-characterized thermoresponsive poly(glycidyl ether) brushes on the cell response at 37 and 20°C...
June 21, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28647524/preparation-and-evaluation-of-bsa-based-hydrosol-nanoparticles-cross-linked-with-genipin-for-oral-administration-of-poorly-water-soluble-curcumin
#15
Narges Shahgholian, Ghadir Rajabzadeh, Bizhan Malaekeh-Nikouei
One of the most interesting functions of albumin is the ability to interact with bioactive compounds. This study describes preparation of protein-based nanoparticles (NPs) for the preparation of solid dispersion of curcumin (CN). Fabrication of hydrosol system of dispersed CN in bovine serum albumin (BSA) was approached, followed by cross-linking with glutaraldehyde (Gta). Response surface methodology (RSM) was used to investigate the influence of input factors (pH, CN content and organic phase ratio (r)), on the particle size and CN entrapment efficiency (EE)...
June 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28647334/test-characteristics-of-milk-amyloid-a-elisa-somatic-cell-count-and-bacteriological-culture-for-detection-of-intramammary-pathogens-that-cause-subclinical-mastitis
#16
S Jaeger, F Virchow, P R Torgerson, M Bischoff, B Biner, S Hartnack, S R Rüegg
Bovine mastitis is an important disease in the dairy industry, causing economic losses as a result of withheld milk and treatment costs. Several studies have suggested milk amyloid A (MAA) as a promising biomarker in the diagnosis of mastitis. In the absence of a gold standard for diagnosis of subclinical mastitis, we estimated the diagnostic test accuracy of a commercial MAA-ELISA, somatic cell count (SCC), and bacteriological culture using Bayesian latent class modeling. We divided intramammary infections into 2 classes: those caused by major pathogens (e...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28647331/combination-of-histidine-lysine-methionine-and-leucine-promotes-%C3%AE-casein-synthesis-via-the-mechanistic-target-of-rapamycin-signaling-pathway-in-bovine-mammary-epithelial-cells
#17
H N Gao, S G Zhao, N Zheng, Y D Zhang, S S Wang, X Q Zhou, J Q Wang
The ratio of different AA in the diets of cows is vital to improve milk protein yield. β-Casein is one of the important milk proteins with high nutritive value. However, the suitable ratio of essential amino acids (EAA) for the expression of β-casein in the immortalized bovine mammary epithelial cell line is not fully characterized. This study employed response surface methodology to determine the optimal ratio of His, Lys, Met, and Leu on β-casein expression level in vitro and clarified the effect of the 4 EAA on β-casein via the mechanistic target of rapamycin (mTOR) signaling pathway...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28647326/effect-of-prepartum-energy-balance-on-neutrophil-function-following-pegbovigrastim-treatment-in-periparturient-cows
#18
S McDougall, S J LeBlanc, A Heiser
Treatment with granulocyte colony-stimulating factor (G-CSF) increases polymorphonuclear cell (neutrophil) count and enhances neutrophil function in the periparturient cow. Prepartum undernutrition was hypothesized to reduce the effect of a commercially available recombinant bovine G-CSF product (pegbovigrastim) on neutrophil count and function. Hence this study was undertaken to test the effect of undernutrition for approximately 1 mo before calving on the innate immune response to pegbovigrastim. Cows (n = 99) on pasture were blocked by expected calving date and body condition score and randomly assigned in a 2 × 2 factorial design...
June 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28647319/in-vitro-stimulation-of-vitellogenin-expression-by-insulin-in-the-mud-crab-scylla-paramamosain-mediated-through-pi3k-akt-tor-pathway
#19
Xiaoshuai Huang, Biyun Feng, Huiyang Huang, Haihui Ye
Vitellogenin (vtg) synthesis, known as vitellogenesis, is one of most important processes in the ovarian development of oviparous animals. Recently, multiple insulin-like peptides (ILPs) have been reported in crustacean species due to the application of transcriptome sequencing. In this context, the present study reports that the addition of an exogenous ILP, bovine insulin, stimulates vtg (termed Sp-vtg) expression in hepatopancreatic explants from the mud crab, Scylla paramamosain, by in vitro experiments...
June 21, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28647287/microwave-thermal-ablation-effects-of-tissue-properties-variations-on-predictive-models-for-treatment-planning
#20
Vanni Lopresto, Rosanna Pinto, Laura Farina, Marta Cavagnaro
Microwave thermal ablation (MTA) therapy for cancer treatments relies on the absorption of electromagnetic energy at microwave frequencies to induce a very high and localized temperature increase, which causes an irreversible thermal damage in the target zone. Treatment planning in MTA is based on experimental observations of ablation zones in ex vivo tissue, while predicting the treatment outcomes could be greatly improved by reliable numerical models. In this work, a fully dynamical simulation model is exploited to look at effects of temperature-dependent variations in the dielectric and thermal properties of the targeted tissue on the prediction of the temperature increase and the extension of the thermally coagulated zone...
June 21, 2017: Medical Engineering & Physics
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