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https://www.readbyqxmd.com/read/28324859/bovine-serum-albumin-as-an-effective-sensitivity-enhancer-for-peptide-based-amperometric-biosensor-for-ultrasensitive-detection-of-prostate-specific-antigen
#1
Zhongxue Tang, Yuanyuan Fu, Zhanfang Ma
Bovine serum albumin (BSA) was firstly implemented as an effective sensitivity enhancer for a peptide-based amperometric biosensor for the ultrasensitive detection of prostate specific antigen (PSA). A porous and conductive substrate of chitosan-lead ferrocyanide-(poly(diallyldimethylammonium chloride)-graphene oxide) was in-situ generated on a glassy carbon electrode (GCE), in which Pb2[Fe(CN)6] served as a novel redox species with strong current signal at -0.46 V (vs. Ag/AgCl). Poly(diallyldimethylammonium chloride)-graphene oxide was applied to improve conductivity of the substrate...
March 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28323915/insulin-igf-1-and-gh-receptors-are-altered-in-an-adipose-tissue-depot-specific-manner-in-male-mice-with-modified-gh-action
#2
Rikke Hjortebjerg, Darlene E Berryman, Ross Comisford, Stuart J Frank, Edward O List, Mette Bjerre, Jan Frystyk, John J Kopchick
Growth hormone (GH) is an important determinant of glucose homeostasis and adipose tissue (AT) function. Using seven-month-old bovine GH transgenic (bGH) mice and GH receptor knockout (GHR-/-) mice, we examined whether changes in GH action affect glucose, insulin, and pyruvate tolerance and AT expression patterns of proteins involved in the interrelated signaling pathways of GH, insulin-like growth factor-1 (IGF-1), and insulin. Furthermore, we searched for AT depot-specific differences in control mice that may explain why GH effects depend on AT location...
February 27, 2017: Endocrinology
https://www.readbyqxmd.com/read/28323870/contribution-of-sortase-srta2-to-lactobacillus-casei-bl23-inhibition-of-staphylococcus-aureus-internalization-into-bovine-mammary-epithelial-cells
#3
Renata F S Souza, Julien Jardin, Chantal Cauty, Lucie Rault, Damien S Bouchard, Luis G Bermúdez-Humarán, Philippe Langella, Vicente Monedero, Núbia Seyffert, Vasco Azevedo, Yves Le Loir, Sergine Even
Probiotics have been considered as a promising strategy to prevent various diseases in both humans and animals. This approach has gained interest in recent years as a potential means to control bovine mastitis. In a previous study, we found that several L. casei strains, including BL23, were able to inhibit the internalization of S. aureus, a major etiologic agent of mastitis, into bovine mammary epithelial cells (bMEC). This antagonism required a direct contact between L. casei and bMEC or S. aureus, suggesting the inhibition relied on interactions between L...
2017: PloS One
https://www.readbyqxmd.com/read/28323836/single-nucleotide-variants-and-indels-identified-from-whole-genome-re-sequencing-of-guzerat-gyr-girolando-and-holstein-cattle-breeds
#4
Nedenia Bonvino Stafuzza, Adhemar Zerlotini, Francisco Pereira Lobo, Michel Eduardo Beleza Yamagishi, Tatiane Cristina Seleguim Chud, Alexandre Rodrigues Caetano, Danísio Prado Munari, Dorian J Garrick, Marco Antonio Machado, Marta Fonseca Martins, Maria Raquel Carvalho, John Bruce Cole, Marcos Vinicius Gualberto Barbosa da Silva
Whole-genome re-sequencing, alignment and annotation analyses were undertaken for 12 sires representing four important cattle breeds in Brazil: Guzerat (multi-purpose), Gyr, Girolando and Holstein (dairy production). A total of approximately 4.3 billion reads from an Illumina HiSeq 2000 sequencer generated for each animal 10.7 to 16.4-fold genome coverage. A total of 27,441,279 single nucleotide variations (SNVs) and 3,828,041 insertions/deletions (InDels) were detected in the samples, of which 2,557,670 SNVs and 883,219 InDels were novel...
2017: PloS One
https://www.readbyqxmd.com/read/28323417/construction-of-a-database-of-collision-cross-section-values-for-glycopeptides-glycans-and-peptides-determined-by-im-ms
#5
Rebecca S Glaskin, Kshitij Khatri, Qi Wang, Joseph Zaia, Catherine E Costello
An ion mobility quadrupole time-of-flight mass spectrometer was used to examine the gas-phase structures of a set of glycopeptides resulting from proteolytic digestion of the well-characterized glycoproteins bovine ribonuclease B, human transferrin, bovine fetuin and human α1-acid glycoprotein, the corresponding deglycosylated peptides and the glycans released by the endoglycosidase PNGase F. When closely related glycoforms did not occur naturally, exoglycosidases were used to achieve stepwise removal of individual saccharide units from the non-reducing termini of the multi-antennary structures...
March 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28319899/molecular-imprinting-sensor-based-on-quantum-weak-measurement
#6
Dongmei Li, Qinghua He, Yonghong He, Meiguo Xin, Yilong Zhang, Zhiyuan Shen
A new type of sensing protocol, based on a high precision metrology of quantum weak measurement, was first proposed for molecularly imprinted polymers (MIP) sensor. The feasibility, sensitivity and selectivity of weak measurement based MIP (WMMIP) sensor were experimentally demonstrated with bovine serum albumin (BSA). Weak measurement system exhibits high sensitivity to the optical phase shift corresponding to the refractive index change, which is induced by the specific capture of target protein molecules with its recognition sites...
March 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28319828/genistein-affects-proliferation-and-migration-of-bovine-oviductal-epithelial-cells
#7
Daniela C García, Pablo A Valdecantos, Dora C Miceli, Mariela Roldán-Olarte
Genistein is one of the most abundant isoflavones in soybean. This molecule induces cell cycle arrest and apoptosis in different normal and cancer cells. Genistein has been of considerable interest due to its adverse effects on bovine reproduction, altering estrous cycle, implantation and fetal development and producing subfertility or infertility. The objective of this work was to study the effects of genistein on the expression of selected genes involved in the regulation of cell cycle and apoptosis. Primary cultures of bovine oviductal epithelial cells (BOEC) were treated with different genistein concentrations (0...
March 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28319472/infective-endocarditis-in-children-an-update
#8
Garth Dixon, Georgi Christov
PURPOSE OF REVIEW: Infective endocarditis in children remains a clinical challenge. Here, we review the impact of the updated 2015 American Heart Association and European Society of Cardiology guidelines on management as well as the significance of the new predisposing factors, diagnostic and treatment options, and the impact of the 2007-2008 change in prophylaxis recommendations. RECENT FINDINGS: The new 2015 infective endocarditis guidelines introduced the endocarditis team, added the new imaging modalities of computer tomography and PET-computer tomography into the diagnostic criteria and endorsed the concept of safety of relatively early surgical treatment...
March 17, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28319364/a-new-spin-on-antibody-drug-conjugates-trastuzumab-fulvestrant-colloidal-drug-aggregates-target-her2-positive-cells
#9
Ahil N Ganesh, Christopher Mclaughlin, Da Duan, Brian K Shoichet, Molly S Shoichet
While the formation of colloidal aggregates leads to artifacts in early drug discovery, their composition makes them attrac-tive as nanoparticle formulations for targeted drug delivery as the entire nanoparticle is composed of drug. The typical tran-sient stability of colloidal aggregates has inhibited exploiting this property. To overcome this limitation, we investigated a series of proteins to stabilize colloidal aggregates of the chemotherapeutic, fulvestrant, including: bovine serum albumin, a generic human immunoglobulin G, and trastuzumab, a therapeutic anti-human epidermal growth factor receptor 2 anti-body...
March 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28319287/iatrogenic-thyrotoxicosis-and-the-role-of-therapeutic-plasma-exchange
#10
Kabeer K Shah, Michael M Mbughuni, Edwin A Burgstaler, Darci R Block, Jeffrey L Winters
Thyroid storm or severe thyrotoxicosis results from extreme thyroid hormone elevation. Therapy includes medical management to prevent hormone production, release, recycling, and peripheral conversion while stabilizing adrenergic tone. Thyroid dysfunction is the usual cause but it can be due to excessive thyroid hormone ingestion. Therapeutic plasma exchange (TPE) has been used to rapidly remove protein-bound thyroid hormone. American Society for Apheresis guidelines make a weak recommendation to perform TPE in selected patients in the treatment of thyrotoxicosis based on low quality evidence...
March 20, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/28319284/advanced-squamous-cell-carcinoma-of-the-axillae-mimicking-hidradenitis-suppurativa
#11
Clio Dessinioti, Mikaella Plaka, Chrisa Zisimou, Eleftheria Christofidou, Christina Antoniou, Alexander J Stratigos
A 56 year-old man presented to our Hidradenitis Clinic with an exophytic, bovine-red, ulcerated plaque in the left axillae (figure 1). He reported that he was diagnosed with hidradenitis suppurativa when this lesion first appeared 8 years ago, as a single small, occasionally purulent, nodule, that had enlarged considerably over the past 7 months. He had previously received oral and topical antibiotics with no improvement. This article is protected by copyright. All rights reserved.
March 20, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28318579/antimicrobial-susceptibility-and-characterization-of-extended-spectrum-%C3%AE-lactamases-in-escherichia-coli-isolated-from-bovine-mastitic-milk-in-south-korea-from-2012-to-2015
#12
Dong-Seob Tark, Dong Chan Moon, Hee Young Kang, Su-Ran Kim, Hyang-Mi Nam, Hee-Soo Lee, Suk-Chan Jung, Suk-Kyung Lim
In this study, we aimed to assess trends in antimicrobial resistance and to investigate the characteristics of extended-spectrum β-lactamase (ESBL)-producing isolates from bovine mastitic milk from 2012 to 2015. A total of 374 Escherichia coli isolates were analyzed (154 in 2012, 113 in 2013, 76 in 2014, and 31 in 2015). No consistent trends in antimicrobial resistance of E. coli isolates occurred during the 4-yr period. The most frequently observed resistance was tetracycline (23.3%), followed by streptomycin (17...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28318575/heritabilities-of-measured-and-mid-infrared-predicted-milk-fat-globule-size-milk-fat-and-protein-percentages-and-their-genetic-correlations
#13
A Fleming, F S Schenkel, A Koeck, F Malchiodi, R A Ali, M Corredig, B Mallard, M Sargolzaei, F Miglior
The objective of this study was to estimate the heritability of milk fat globule (MFG) size and mid-infrared (MIR) predicted MFG size in Holstein cattle. The genetic correlations between measured and predicted MFG size with milk fat and protein percentage were also investigated. Average MFG size was measured in 1,583 milk samples taken from 254 Holstein cows from 29 herds across Canada. Size was expressed as volume moment mean (D[4,3]) and surface moment mean (D[3,2]). Analyzed milk samples also had average MFG size predicted from their MIR spectral records...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28318066/three-dimensional-ultrashort-echo-time-cones-t1%C3%AF-3d-ute-cones-t1%C3%AF-imaging
#14
Ya-Jun Ma, Michael Carl, Hongda Shao, Anthony S Tadros, Eric Y Chang, Jiang Du
We report a novel three-dimensional (3D) ultrashort echo time (UTE) sequence employing Cones trajectory and T1ρ preparation (UTE-Cones-T1ρ ) for quantitative T1ρ assessment of short T2 tissues in the musculoskeletal system. A basic 3D UTE-Cones sequence was combined with a spin-locking preparation pulse for T1ρ contrast. A relatively short TR was used to decrease the scan time, which required T1 measurement and compensation using 3D UTE-Cones data acquisitions with variable TRs. Another strategy to reduce the total scan time was to acquire multiple Cones spokes (Nsp ) after each T1ρ preparation and fat saturation...
March 20, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28317992/synthetic-glycoconjugates-characterize-the-fine-specificity-of-brucella-a-and-m-monoclonal-antibodies
#15
Satadru Sekhar Mandal, N Vijaya Ganesh, Joanna M Sadowska, David R Bundle
The dominant cell wall antigen of Brucella bacteria is the O-polysaccharide component of the smooth lipopolysaccharide. Infection by various Brucella biovars causes abortions and infertility in a wide range of domestic and wild animals and debilitating disease in humans. Diagnosis relies on the detection of antibodies to the A and M antigens expressed in the O-polysaccharide. This molecule is a homopolymer of the rare monosaccharide, 4-formamido-4,6-dideoxy-d-mannopyranose (Rha4NFo). The A epitope is created by a uniform α1,2 linked internal polymeric sequence capped by a distinct tetrasaccharide sequence defining the M antigen...
March 20, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28317898/microrna-roles-in-signalling-during-lactation-an-insight-from-differential-expression-time-course-and-pathway-analyses-of-deep-sequence-data
#16
Duy N Do, Ran Li, Pier-Luc Dudemaine, Eveline M Ibeagha-Awemu
The study examined microRNA (miRNA) expression and regulatory patterns during an entire bovine lactation cycle. Total RNA from milk fat samples collected at the lactogenesis (LAC, day1 [D1] and D7), galactopoiesis (GAL, D30, D70, D130, D170 and D230) and involution (INV, D290 and when milk production dropped to 5 kg/day) stages from 9 cows was used for miRNA sequencing. A total of 475 known and 238 novel miRNAs were identified. Fifteen abundantly expressed miRNAs across lactation stages play regulatory roles in basic metabolic, cellular and immunological functions...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28317806/mycobacterium-lepraemurium-uses-tlr-6-and-mr-but-not-lipid-rafts-or-dc-sign-to-gain-access-into-mouse-macrophages
#17
Mayra Silva-Miranda, Patricia Arce-Paredes, Oscar Rojas-Espinosa
OBJECTIVE/BACKGROUND: Mycobacterium lepraemurium (MLM), the etiologic agent of murine leprosy, is an intracellular parasite of macrophages; the mechanism used by this bacterium to enter macrophages is not known. The fate of the MLM phagosome inside macrophages is also unknown. This study was conducted to investigate how MLM enters macrophages and to define the maturation process of MLM phagosome inside macrophages. MATERIALS AND METHODS: Peritoneal macrophages were incubated in the presence of mannan-bovine serum albumin (BSA), and antibodies to known macrophage receptors, including, anti-FcγRIII/RII (anti-CD16/32), anti-CD35 (anti-CR1), anti-TLR2, anti-TLR4, anti-TLR6, anti-CD14, and anti-dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN)...
January 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28317723/multi-spectroscopic-and-molecular-modeling-studies-of-bovine-serum-albumin-interaction-with-sodium-acetate-food-additive
#18
Hossein Mohammadzadeh-Aghdash, Jafar Ezzati Nazhad Dolatabadi, Parvin Dehghan, Vahid Panahi-Azar, Abolfazl Barzegar
Sodium acetate (SA) has been used as a highly effective protectant in food industry and the possible effect of this additive on the binding to albumin should be taken into consideration. Therefore, for the first time, the mechanism of SA interaction with bovine serum albumin (BSA) has been investigated by multi-spectroscopic and molecular modeling methods under physiological conditions. Stern-Volmer fluorescence quenching analysis showed an increase in the fluorescence intensity of BSA upon increasing the amounts of SA...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317153/pharmacokinetics-and-pulmonary-distribution-of-gamithromycin-after-intravenous-administration-in-foals
#19
S Berlin, T Randow, E Scheuch, M Grube, M Venner, W Siegmund
The long-acting azalide antibiotic gamithromycin is marketed for intramuscular treatment of bovine and swine infections. Off-label use in foals leads to severe local lesions likely caused by hyperosmolality of the injected solution. We provide evidence from a pharmacokinetic study in 10 warm-blooded healthy foals for intravenous bolus injection of gamithromycin diluted in distilled water to be a safe and well tolerated alternative. By intravenous dosing, markedly higher plasma exposure and better penetration into bronchoalveolar lavage cells but lower distribution into epithelial lining fluid are achieved as after intramuscular or subcutaneous administration...
March 19, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28316987/selective-removal-of-hemoglobin-from-blood-using-hierarchical-copper-shells-anchored-to-magnetic-nanoparticles
#20
Youxun Liu, Yaokun Wang, Mingyang Yan, Juan Huang
Hierarchical copper shells anchored on magnetic nanoparticles were designed and fabricated to selectively deplete hemoglobin from human blood by immobilized metal affinity chromatography. Briefly, CoFe2O4 nanoparticles coated with polyacrylic acid were first synthesized by a one-pot solvothermal method. Hierarchical copper shells were then deposited by immobilizing Cu(2+) on nanoparticles and subsequently by reducing between the solid CoFe2O4@COOH and copper solution with NaBH4. The resulting nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, and vibrating sample magnetometry...
2017: BioMed Research International
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