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https://www.readbyqxmd.com/read/29050430/extraction-and-physicochemical-characterization-of-broiler-gallus-gallus-domesticus-skin-gelatin-compared-to-commercial-bovine-gelatin
#1
E Aykin-Dinçer, A Koç, M Erbas
Gelatin was extracted from broiler (Gallus gallus domesticus) skins and analyzed to compare its physicochemical properties with those of commercial bovine gelatin. The average yield of broiler skin gelatin was 6.5% on a wet weight basis. Broiler skin gelatin had more α1-and α2-chains than β-chain and contained high molecular weight (γ-chain) polymers. Glycine was the dominant amino acid in broiler skin gelatin (20.26%), followed by proline (Pro) (15.12%) then hydroxyproline (Hyp) (11.36%). Compared to commercial bovine gelatin, broiler skin gelatin had less total imino acids (Pro and Hyp) but a higher (33...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29049912/globular-chitosan-treatment-of-bovine-jugular-veins-evidence-of-anticalcification-efficacy-in-the-subcutaneous-rat-model
#2
Anna A Krasilnikova, David S Sergeevichev, Vladislav V Fomenko, Alexander A Korobeynikov, Maria B Vasilyeva, Alexander S Yunoshev, Alexander M Karaskov, Evgeny A Pokushalov
Vascular xenografts are widely used in cardiovascular surgery as an alternative to autologous vessels and vascular allografts. Calcification is one of the main drawbacks of vascular grafts, especially among young patients and children. Among different anticalcification approaches, chitosan emerges as a highly promising candidate due to its versatility, natural origin, and biocompatibility. We investigated the anticalcification efficacy of globular chitosan ("Chitozol") as it demonstrated the improved rate of water solubility as compared with conventional linear macromolecules of chitosan...
September 14, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/29049705/follow-up-of-a-simple-method-for-aortic-valve-reconstruction-with-fixed-pericardium-in-children
#3
Amir-Reza Hosseinpour, Alejandro Adsuar-Gómez, Antonio González-Calle, Antonio Moruno-Tirado, Francisco García-Angleu, Israel Valverde, Felix Coserria-Sánchez, Begoña Manso-García
In 2013, we published a simple method for aortic valve reconstruction in children using fixed pericardium in this journal. This was not expected to be a durable solution but a temporizing measure in the absence of other solutions, in order to buy time for growth that would allow subsequent prosthetic valve insertion. As such, this was implemented in 3 patients with excellent immediate results, as reported in our original publication. We are now writing to provide longer term information. In 2 cases, a newborn and a 12-year-old boy, the valve calcified heavily at 4 years' follow-up...
July 21, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049662/flexible-mechanoprosthesis-made-from-woven-ultra-high-molecular-weight-polyethylene-fibres-proof-of-concept-in-a-chronic-sheep-model
#4
Amir Basir, Remco B Grobben, Maarten Jan Cramer, Joost A van Herwaarden, Aryan Vink, Gerard Pasterkamp, Jolanda Kluin, Paul F Gründeman
OBJECTIVES: Ultra-high-molecular-weight polyethylene (UHMWPE) fibres are flexible, have high tensile strength, and platelet and bacterial adhesion is low. Therefore, UHMWPE may overcome limitations of current mechanical valves and bioprostheses. In this study, the biocompatibility and functionality of prototype handmade stented valves from woven UHMWPE (U-valve) was assessed in a chronic sheep model with acetylsalicylic acid monotherapy. METHODS: Native pulmonary valves of 23 sheep were replaced by U-valves ( n  = 18) or Perimount bovine bioprostheses (reference group, n  = 5)...
July 26, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049564/a-new-technique-to-implant-a-transcatheter-inflatable-fully-repositionable-prosthesis-in-aortic-stenosis-with-severe-asymmetric-calcification
#5
Hasan Bushnaq, Christoph Raspé, Alper Öner, Seyrani Yücel, Hüseyin Ince, Sebastian-Patrick Sommer
OBJECTIVES: In contrast to stented transcatheter aortic valves, the Direct Flow Medical (DFM) valve is a stentless bovine aortic bioprosthesis mounted in a non-metallic inflatable frame. Hence, severe asymmetric annular calcification may result in residually elevated transaortic pressure gradients after DFM implantation. We present a novel intraprocedural dilatation (IDIL) technique for successful implantation of the DFM valve in the presence of complex annular calcification. METHODS: Between January 2014 and May 2015, 55 patients underwent DFM valve-based transcatheter aortic valve implantation at our institution...
August 1, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049314/evaluation-of-characteristic-of-human-turbinate-derived-mesenchymal-stem-cells-cultured-in-the-serum-free-media
#6
Se Hwan Hwang, WeonSun Lee, Sang Hi Park, Hee Jin Lee, Sun Hwa Park, Dong Chang Lee, Mi Hyun Lim, Sang A Back, Byeong Gon Yun, Jung Ho Jeun, Jung Yeon Lim, Jun Myung Kang, Sung Won Kim
We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM...
2017: PloS One
https://www.readbyqxmd.com/read/29048834/-preparation-and-performance-characterization-of-gold-nanoparticles-modified-chiral-capillary-electrochromatography-stationary-phase
#7
Lele Xiong, Ruijun Li, Yibing Ji
Gold nanoparticles (GNPs, 15 nm) were prepared and introduced to amino groups derived silica monolithic column. Bovine serum albumin (BSA) was immobilized via covalent modification method onto the carboxylic functionalized GNPs to afford chiral stationary phase (CSP) for enantioseparation. GNPs were well dispersed and successfully incorporated onto the columns with the contents as high as 17.18% by characterization method such as transmission electron microscopy (TEM), ultraviolet (UV)-visible absorption spectra and scanning electron microscopy (SEM)...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048801/-determination-of-thyreostats-in-bovine-urine-using-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#8
Rodziewicz Lech, MasŁOwiecka Jolanta, Sadowska Anna, Car Halina
Five thyreostats (TSs), namely tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil, were determined in bovine urine using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) in positive electrospray ionization mode. Extraction and clean-up were achieved using a ChemElut cartridge with tert-butyl methyl ether, without a derivatization step. Separation was achieved on an Acquity UPLC SS T3 column. The mobile phase was acetonitrile and water containing 0...
October 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048483/draft-genome-of-the-gayal-bos-frontalis
#9
Ming-Shan Wang, Yan Zeng, Xiao Wang, Wen-Hui Nie, Jin-Huan Wang, Wei-Ting Su, Newton O Otecko, Zi-Jun Xiong, Sheng Wang, Kai-Xing Qu, Shou-Qing Yan, Min-Min Yang, Wen Wang, Yang Dong, Dong-Dong Wu, Ya-Ping Zhang
Background: Gayal (Bos frontalis), also known as mithan or mithun, is a large endangered semi-domesticated bovine that has a limited geographical distribution in the hill-forests of China, Northeast India, Bangladesh, Myanmar, and Bhutan. Many questions about the gayal such as its origin, population history as well as genetic basis of local adaptation remain largely unresolved. De novo sequencing and assembly of the whole gayal genome provides an opportunity to address these issues. Findings: We report a high-depth sequencing, de novo assembly, and annotation of a female Chinese gayal genome...
October 5, 2017: GigaScience
https://www.readbyqxmd.com/read/29047008/pastoralists-knowledge-and-practices-towards-clinical-bovine-dermatophilosis-in-cattle-herds-of-north-central-nigeria-the-associated-factors-burden-and-economic-impact
#10
Nma Bida Alhaji, Tajudeen Opeyemi Isola
Dermatophilosis is a contagious disease of high economic importance. The study assessed knowledge/awareness and clinical burden of and mitigation measures practised against clinical bovine dermatophilosis in pastoral herds of North-Central Nigeria and associated socio-cultural factors that predisposed to its occurrence in herds. A cross-sectional survey was conducted between October 2015 and September 2016. Questionnaires were administered on pastoralists and clinical assessment of cattle with gross pathological lesions indicative of dermatophilosis conducted...
October 18, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29046740/draft-genome-sequence-and-characterization-of-commensal-escherichia-coli-strain-bg1-isolated-from-bovine-gastro-intestinal-tract
#11
Audrey Segura, Pauline Auffret, Christophe Klopp, Yolande Bertin, Evelyne Forano
Escherichia coli is the most abundant facultative anaerobic bacteria in the gastro-intestinal tract of mammals but can be responsible for intestinal infection due to acquisition of virulence factors. Genomes of pathogenic E. coli strains are widely described whereas those of bovine commensal E. coli strains are very scarce. Here, we report the genome sequence, annotation, and features of the commensal E. coli BG1 isolated from the gastro-intestinal tract of cattle. Whole genome sequencing analysis showed that BG1 has a chromosome of 4,782,107 bp coding for 4465 proteins and 97 RNAs...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/29046497/hypoxia-increases-glucose-transporter-1-expression-in-bovine-corpus-luteum-at-the-early-luteal-stage
#12
Ryo Nishimura, Hiroki Hasegawa, Masamichi Yamashita, Norihiko Ito, Yoshiharu Okamoto, Takashi Takeuchi, Tomoaki Kubo, Kosuke Iga, Koji Kimura, Mitsugu Hishinuma, Kiyoshi Okuda
A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells...
October 17, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29046443/prion-protein-devoid-of-the-octapeptide-repeat-region-delays-bse-pathogenesis-in-mice
#13
Hideyuki Hara, Hironori Miyata, Nandita Rani Das, Junji Chida, Tatenobu Yoshimochi, Keiji Uchiyama, Hitomi Watanabe, Gen Kondoh, Takashi Yokoyama, Suehiro Sakaguchi
Conformational conversion of the cellular isoform of prion protein PrP(C), into the abnormally folded, amyloidogenic isoform, PrP(Sc), is a key pathogenic event in prion diseases including Creutzfeldt-Jakob disease in humans and scrapie and bovine spongiform encephalopathy (BSE) in animals. We previously reported that the octapeptide repeat (OR) region could be dispensable for converting PrP(C) into PrP(Sc) after infection with RML prions. We demonstrated that mice transgenically expressing mouse PrP with deletion of the OR region on the PrP-knockout background, designated Tg(PrPΔOR)/Prnp(0/0) mice, did not reduce susceptibility to RML scrapie prions, with abundant accumulation of PrP(Sc)ΔOR in their brains...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29045929/deciphering-the-complexation-process-of-a-fluoroquinolone-antibiotic-levofloxacin-with-bovine-serum-albumin-in-the-presence-of-additives
#14
Amandeep Kaur, Imran Ahmd Khan, Parampaul Kaur Banipal, Tarlok Singh Banipal
The current work aims to explore the thermodynamic and conformational aspects for the binding of fluoroquinolone antibacterial drug, levofloxacin (LFC), with bovine serum albumin (BSA) using calorimetric, spectroscopic (UV-visible, fluorescence, circular dichroism, and (1)H NMR), dynamic light scattering (DLS) and computational methods (molecular docking). The binding of LFC with BSA at two sequential sites with higher affinity (~10(3)M(-1)) at the first site has been explored by calorimetry whereas the binding at a single site with affinity of the order of ~10(4)M(-1) has been observed from fluorescence spectroscopy...
October 9, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29045588/the-mycotoxin-metabolite-deepoxy-deoxynivalenol-increases-apoptosis-and-decreases-steroidogenesis-in-bovine-ovarian-theca-cells
#15
Hilda M Guerrero-Netro, Anthony Estienne, Younès Chorfi, Christopher A Price
The mycotoxin deoxynivalenol (DON) has been shown to inhibit ovarian granulosa cell function in cattle in vitro, but it is not known whether DON or its metabolite deepoxy-DON (DOM-1) affect theca cell function. The objectives of the present study were to determine the effects of DON and of DOM-1 on theca cell steroidogenesis and apoptosis, and to determine the main pathways through which they act. Bovine theca cells were cultured in a non-luteinizing serum-free culture system, and challenged with DON or DOM-1 for 4 d to measure steroidogenesis and apoptosis, for 1-8 h to measure immediate-early genes, and for 5-60 min to measure phosphorylation of intracellular signaling proteins...
October 13, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29044517/circfut10-reduces-proliferation-and-facilitates-differentiation-of-myoblasts-by-sponging-mir-133a
#16
Hui Li, Jiameng Yang, Xuefeng Wei, Chengchuang Song, Dong Dong, Yongzhen Huang, Xianyong Lan, Martin Plath, Chuzhao Lei, Yun Ma, Xinglei Qi, Yueyu Bai, Hong Chen
Circular RNAs (circRNAs) have been identified in various tissues and cell types from human, monkey, porcine and mouse. However, knowledge on circRNAs in bovine muscle development is limited. We downloaded and analyzed the circRNAs sequencing data of bovine skeletal muscle tissue, and further characterized the role of a candidate circRNA (circFUT10) in muscle development. Quantitative real-time PCR (qPCR) and western blot assays were used to confirm the expression of genes involved in myoblasts differentiation and proliferation...
October 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29044427/genomic-identification-expression-profiling-and-functional-characterization-of-catsper-channels-in-the-bovine
#17
Gillian P Johnson, Anne-Marie English, Sinead Cronin, David A Hoey, Kieran G Meade, Sean Fair
Cation channels of sperm (CatSper) are sperm-specific calcium channels with identified roles in the regulation of sperm function in humans, mice, and horses. We sought to employ a comparative genomics approach to identify conserved CATSPER genes in the bovine genome, and profile their expression in reproductive tissue. We hypothesized that CATSPER proteins expressed in bull testicular tissue mediates sperm hyperactivation and their rheotactic response in the reproductive tract of the cow. Bioinformatic analysis identified all four known CATSPER genes (CATSPER 1-4) in the bovine genome, and profiling by quantitative real-time polymerase chain reaction identified site-specific variation in messenger ribonucleic acid (mRNA) expression for all four genes along the reproductive tract of the bull...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29044423/cultured-bovine-embryo-biopsy-conserves-methylation-marks-from-original-embryo
#18
Noelia Fonseca Balvís, Soledad Garcia-Martinez, Serafín Pérez-Cerezales, Elena Ivanova, Isabel Gomez-Redondo, Meriem Hamdi, Dimitrios Rizos, Pilar Coy, Gavin Kelsey, Alfonso Gutierrez-Adan
A major limitation of embryo epigenotyping by chromatin immunoprecipitation analysis is the reduced amount of sample available from an embryo biopsy. We developed an in vitro system to expand trophectoderm cells from an embryo biopsy to overcome this limitation. This work analyzes whether expanded trophectoderm (EX) is representative of the trophectoderm (TE) methylation or adaptation to culture has altered its epigenome. We took a small biopsy from the trophectoderm (30-40 cells) of in vitro produced bovine-hatched blastocysts and cultured it on fibronectin-treated plates until we obtained ∼4 × 104 cells...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29044318/antibiotic-activity-of-plectranthus-ornatus-codd-a-traditional-medicinal-plant
#19
Fernanda R Nascimento, Kamylla R S Albuquerque, Marcos R Oliveira, Virginia R Pizziolo, Beatriz G Brasileiro, Gaspar Diaz, Marisa A N Diaz
The dichloromethane extract of Plectranthus ornatus Codd., a tradicional medicinal plant, showed antibiotic activity with minimum inhibitory concentration (MIC) values of 0.4 mg.mL-1 and 100 percent of biofilm inhibition against Staphylococcus aureus strains isolated from animals with mastitis infections. Based on these antibacterial activities, in addition to ethnopharmacological reports from healing men and farmers in Brazil, an herbal soap was produced from this active extract and was tested both in vitro and in vivo...
October 16, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29042926/anti-egfr-antibody-conjugated-silica-nanoparticles-as-probes-for-lung-cancer-detection
#20
Jun Wan, Wei Wu, Renquan Zhang, Shandong Liu, Yunlong Huang
A well-designed nanosystem [anti-epidermal growth factor receptor-MB-encapsulated thiol-terminated silica nanoparticles (EGFR/MB-SHSi) complexes] containing silica nanoparticles and near-infrared fluorescence dye (NIRF) methylene blue (MB) was established as a tumor-targeted probe for potential lung cancer detection. The anti-EGFR/MB-SHSi complexes exhibited desirable and homogenous particle size, high bovine serum albumin stability, low hemolytic activity, neutral surface charges and negligible cytotoxicity in vitro...
October 2017: Experimental and Therapeutic Medicine
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