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https://www.readbyqxmd.com/read/28823107/fluorescent-labeling-of-protein-using-blue-emitting-8-amino-bodipy-derivatives
#1
Dokyoung Kim, Donghee Ma, Muwoong Kim, Yuna Jung, Na Hee Kim, Chiho Lee, Seo Won Cho, Sungnam Park, Youngbuhm Huh, Junyang Jung, Kyo Han Ahn
8-Amino-BODIPY (boron-dipyrromethane) dyes show bright blue fluorescence. Disclosed here are synthesis and characterization of the photophysical properties of a series of functionalized 8-Amino-BODIPY (BP1-4) for protein labeling. The compact structure and solvent-insensitive absorption property of the dye are desirable features for protein labeling. For the model protein, bovine serum albumin (BSA), the labeling proceeds under mild condition via amide bond formation or thiol-ene conjugation with maintaining the bright blue fluorescence...
August 19, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28823085/chromaffin-cells-as-a-model-to-evaluate-mechanisms-of-cell-death-and-neuroprotective-compounds
#2
REVIEW
Cristobal de Los Rios, Maria F Cano-Abad, Mercedes Villarroya, Manuela G López
In this review, we show how chromaffin cells have contributed to evaluate neuroprotective compounds with diverse mechanisms of action. Chromaffin cells are considered paraneurons, as they share many common features with neurons: (i) they synthesize, store, and release neurotransmitters upon stimulation and (ii) they express voltage-dependent calcium, sodium, and potassium channels, in addition to a wide variety of receptors. All these characteristics, together with the fact that primary cultures from bovine adrenal glands or chromaffin cells from the tumor pheochromocytoma cell line PC12 are easy to culture, make them an ideal model to study neurotoxic mechanisms and neuroprotective drugs...
August 19, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28823007/excretory-secretory-products-from-two-fasciola-hepatica-isolates-induce-different-transcriptional-changes-and-il-10-release-in-lps-activated-bovine-boma-macrophages
#3
Piotr Bąska, Luke James Norbury, Anna Zawistowska-Deniziak, Marcin Wiśniewski, Kamil Januszkiewicz
Fasciola hepatica are trematodes that reside in the bile ducts of mammals. Infection causes US$3 billion in losses annually in animal production and is considered a zoonosis of growing importance. An under-represented area in F. hepatica research has been the examination of the different immunomodulatory abilities of various parasite isolates on the host immune system. In this paper, this issue was explored, with the bovine macrophage cell line "BOMA". The cells were matured by LPS treatment and stimulated with excretory/secretory antigens (ES) from two Fasciola hepatica isolates: a laboratory isolate "Weybridge" (Fh-WeyES) and a wild isolate (Fh-WildES)...
August 19, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28822848/tetracarboxy-phthalocyanines-from-excited-state-dynamics-to-photodynamic-inactivation-against-bovine-herpesvirus-type-1
#4
Leandro H Z Cocca, Taise M A Oliveira, Fernando Gotardo, Amanda V Teles, Ricardo Menegatti, Jonathas P Siqueira, Cleber R Mendonça, Luiz A M Bataus, Anderson O Ribeiro, Thalita F M Souza, Guilherme R L Souza, Pablo J Gonçalves, Leonardo De Boni
Herein we present the excited state dynamic of zinc and aluminum tetracarboxy-phthalocyanines (ZnPc and AlPc) and its application in the photodynamic inactivation (PDI) of Bovine herpesvirus type 1 (BoHV-1) in vitro. The excited state dynamic provides valuable data to describe the excited state properties of potential optical limiters and/or photosensitizers (PSs), such as: the excited state cross-sections, fluorescence lifetime and triplet state quantum yield. The excited state characterization was performed using three different Z-scan techniques: Single Pulse, White Light Continuum and Pulse Train...
August 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28822723/effect-of-religious-belief-on-selecting-of-graft-materials-used-in-oral-and-maxillofacial-surgery
#5
Zeynep Güngörmüş, Metin Güngörmüş
PURPOSE: Various graft materials, such as synthetic and biological products, are used routinely in maxillofacial surgery. These materials are usually derived from porcine, bovine, and human tissues; some religious beliefs forbid the dietary use of substances from certain animal sources. The aim of this study was to evaluate the effect of religious belief on selecting different graft types used in maxillofacial surgery. MATERIALS AND METHODS: In total, 203 participants were included in this survey...
July 25, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28822542/short-communication-a-study-of-lactobacillus-isolates-adherence-to-and-influence-on-membrane-integrity-of-human-caco-2-cells
#6
Neethu M Jose, Craig R Bunt, Arlene McDowell, Jasper Z S Chiu, Malik A Hussain
The selection criteria of ideal probiotic bacteria are complex and involve many factors. One key criterion is based on the ability of the probiotic bacteria to adhere to the epithelial lining of the gastrointestinal tract. The objective of this study was to evaluate and compare the adherence and influence on membrane integrity of 2 selected lactobacilli isolates-Lactobacillus rhamnosus MI13 (dairy food origin) and L. plantarum RC2 (bovine rumen origin)-to Caco-2 cells in the presence and absence of Escherichia coli...
August 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28822242/mucosal-immunity-of-the-postpartum-bovine-genital-tract
#7
REVIEW
Dinesh Dadarwal, Colin Palmer, Philip Griebel
In this review, mucosal immune defense mechanisms used to control infections in the bovine genital tract (vestibule, vagina, cervix, uterus and oviduct) during the postpartum period are reviewed. Knowledge gaps are highlighted to emphasize the need for further investigations. Physical barriers to the entry of microbes include vulvar sealing, vestibule-vaginal constriction, a narrow cervical opening and the mucosal epithelium along with the overlying mucus layer. Genital tract mucosal epithelial cells recognize damage-associated molecular patterns and pathogen-associated molecular patterns and respond by secreting antimicrobial peptides and cytokines to recruit and activate immune cells...
August 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822192/-taurine-affects-expression-of-icam-1-vcam-1-by-p38-pathway-in-hypoxic-endothelial-cells
#8
Xiao-Dan Zhang, Shu-Ya Zhao, Yun-Lin Li, Da-Ling Zhu, Ru Zhang, Jie-Jing Sheng, Shu-Jing Wang
To investigate the effect of taurine(Tau) on ICAM-1, VCAM-1 by p-p38 pathway in bovine pulmonary artery endothelial cells(PAECs) and explore its mechanism of action. Generation 4-12 cells in primary cultures of PAECs were used in experiments and divided into five groups: control group, hypoxia(hyp) group, inhibitor(SB203580) group, treatment(Tau) group, and treatment+inhibitor(SB+Tau) group. The concentration of Tau:100 mmol•L⁻¹; p38 inhibitor SB203580: 20 μmol•L⁻¹; and the treatment time was 12 h...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28821957/co-culture-of-chondrons-and-mesenchymal-stromal-cells-reduces-the-loss-of-collagen-vi-and-improves-extracellular-matrix-production
#9
H A Owida, T De Las Heras Ruiz, A Dhillon, Y Yang, N J Kuiper
Adult articular chondrocytes are surrounded by a pericellular matrix (PCM) to form a chondron. The PCM is rich in hyaluronan, proteoglycans, and collagen II, and it is the exclusive location of collagen VI in articular cartilage. Collagen VI anchors the chondrocyte to the PCM. It has been suggested that co-culture of chondrons with mesenchymal stromal cells (MSCs) might enhance extracellular matrix (ECM) production. This co-culture study investigates whether MSCs help to preserve the PCM and increase ECM production...
August 19, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28821698/schmallenberg-virus-infection-in-scottish-cattle
#10
(no author information available yet)
Schmallenberg virus infection on cattle farms in southern ScotlandOutbreaks of infectious bovine rhinotracheitisSalmonella Bovismorbificans infection in cattle and sheepGastric ulceration in pigsTrichomonosis in a sparrowhawk and a hen harrierThese are among matters discussed in the disease surveillance report for April 2017 from SAC Consulting: Veterinary Services (SAC C VS).
August 19, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28821452/pinocembrin-a-novel-histidine-decarboxylase-inhibitor-with-anti-allergic-potential-in-in-vitro
#11
Hamza Hanieh, Villianur Ibrahim Hairul Islam, Subramanian Saravanan, Muthiah Chellappandian, Kessavane Ragul, Arumugam Durga, Kaliyamoorthy Venugopal, Venugopal Senthilkumar, Palanisamy Senthilkumar, Krishnaraj Thirugnanasambantham
Pinocembrin (5, 7- dihydroxy flavanone) is the most abundant chiral flavonoid found in propolis, exhibiting antioxidant, antimicrobial and anti-inflammatory properties. However, the effect of Pinocembrin on allergic response is unexplored. Thus, current study aimed at investigating the effects of Pinocembrin on IgE-mediated allergic response in vitro. A special emphasis was directed toward histidine decarboxylase (HDC) and other pro-allergic and pro-inflammatory mediators. Preliminary studies, using a microbiological model of Klebsiella pneumoniae, provided first evidences that suggest Pinocembrin as a potential thermal stable inhibitor for HDC...
August 15, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28821058/exploration-of-permeability-and-antifouling-performance-on-modified-cellulose-acetate-ultrafiltration-membrane-with-cellulose-nanocrystals
#12
Jinling Lv, Guoquan Zhang, Hanmin Zhang, Fenglin Yang
Cellulose nanocrystals (CNCs) were introduced into cellulose diacetate (CDA) matrix via immerged phase-inversion process, aiming to improve the filtration and antifouling performance of CNCs/CDA blending membrane. The effects of CNCs on membrane morphologies, hydrophilicity, permeability and antifouling property were investigated. Results showed that the incorporation of CNCs into CDA membrane could effectively enhance the permeability and antifouling property of CNCs/CDA blending membrane by optimizing membrane microstructure and improving membrane hydrophilicity...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821047/structural-analysis-and-immunomodulatory-effect-of-polysaccharide-from-atractylodis-macrocephalae-koidz-on-bovine-lymphocytes
#13
Wei Xu, Ran Guan, Fushan Shi, Aifang Du, Songhua Hu
RAMP60, RAMP70, RAMP80 and RAMPtp extracted from the rhizome of Atractylodis macrocephalae Koidz. were chemically analyzed and tested for their lymphocyte stimulation. Among them, only RAMPtp was found to induce proliferation of lymphocytes from bovine supramammary lymph node (SMLN). It was found that RAMPtp was homogeneous polysaccharide with Mn of 1.867×10(3) by GPC analysis, composed of glucose, mannose, rhamnose, arabinose and galactose, with mass percentages of 60.67%, 14.99%, 10.61%, 8.83% and 4.90%, respectively by HPLC analysis, connected by 1, 3-linked β-D Galp and 1, 6-linked β-D Galp residues by NMR analysis and was amorphous in structure by SEM analysis...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820976/a-hydrophobic-organelle-probe-based-on-aggregation-induced-emission-nanosuspension-preparation-and-direct-use-for-endoplasmic-reticulum-imaging-in-living-cells
#14
Sichao Zheng, Cuihong Huang, Xuyan Zhao, Yong Zhang, Shuwen Liu, Qiuhua Zhu
Organic fluorophores have a wide range of biological uses and are usually needed to be prepared as water-soluble compounds or nanoparticles for applications in aqueous biosystems owing to their hydrophobic properties, which often is a complex, time-consuming and high-cost process. Here, the nanoparticle preparation of hydrophobic fluorophores and their application in cell imaging have been investigated. It was found: a) fetal bovine serum (FBS) shows an excellent dispersion effect on hydrophobic small-molecule organic compounds; b) a hydrophobic C6-unsubstituted tetrahydropyrimidine (Me-THP-Naph) can be prepared as nanosuspensions utilizing cell culture medium with 10% FBS and directly be used as a specific real-time imaging probe for the endoplasmic reticulum (ER), a dynamic organelle playing a crucial role in many cellular processes...
August 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28820424/observation-of-self-polarization-in-the-bsa-protected-au20-clusters
#15
Deepak Kumar Swain, Alongbar Narzary, Anil Singh, Amreesh Chandra, T Nagasawa, S Yamamoto, Masaya Mitsuishi, S Rath
The bovine serum albumin (BSA) protected gold clusters (atomicity ~ 20), prepared using a wet chemical route, show a strong dipolar radiative transition with gap energy of 1.93 eV due to the high oscillator strength as confirmed from the emission studies. Self-arrangement of the clusters having fixed atomicity yields a low dispersive dielectric and electric self-polarization nature. The electrical hysteresis loop measurements returned a remanent polarization as 0.05 µC/cm2 which can be correlated with dipolar orientation (activation energy ~ 45...
August 18, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28820136/protective-effect-of-val129-prp-against-bovine-spongiform-encephalopathy-but-not-variant-creutzfeldt-jakob-disease
#16
Natalia Fernández-Borges, Juan Carlos Espinosa, Alba Marín-Moreno, Patricia Aguilar-Calvo, Emmanuel A Asante, Tetsuyuki Kitamoto, Shirou Mohri, Olivier Andréoletti, Juan María Torres
Bovine spongiform encephalopathy (BSE) is the only known zoonotic prion that causes variant Creutzfeldt-Jakob disease (vCJD) in humans. The major risk determinant for this disease is the polymorphic codon 129 of the human prion protein (Hu-PrP), where either methionine (Met129) or valine (Val129) can be encoded. To date, all clinical and neuropathologically confirmed vCJD cases have been Met129 homozygous, with the exception of 1 recently reported Met/Val heterozygous case. Here, we found that transgenic mice homozygous for Val129 Hu-PrP show severely restricted propagation of the BSE prion strain, but this constraint can be partially overcome by adaptation of the BSE agent to the Met129 Hu-PrP...
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28819881/resveratrol-induces-apoptosis-and-inhibits-adipogenesis-by-stimulating-the-sirt1-ampk%C3%AE-foxo1-signalling-pathway-in-bovine-intramuscular-adipocytes
#17
Xiaomu Liu, Hongbo Zhao, Qing Jin, Wei You, Haijian Cheng, Yifan Liu, Enliang Song, Guifen Liu, Xiuwen Tan, Xianglun Zhang, Fachun Wan
Sirtuin type 1 (SIRTl) and AMP-activated protein kinase (AMPK) play important roles in regulating energy metabolism, cell proliferation and differentiation, ageing, apoptosis, and metabolism. The effect of 100, 200, and 400 μm Resveratrol (RES), an activator of SIRT1, on apoptosis of bovine intramuscular adipocytes was investigated by nuclear staining, flow cytometry, quantitative real-time polymerase chain reaction, and western blotting. Results show that RES inhibited adipogenesis, decreased cell viability, and increased apoptotic rates in a dose-dependent way...
August 17, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28819879/lower-extremity-bypass-using-bovine-carotid-artery-graft-artegraft-an-analysis-of-124-cases-with-long-term-results
#18
Philip Lindsey, Angela Echeverria, Mathew Cheung, Elias Kfoury, Carlos F Bechara, Peter H Lin
INTRODUCTION: Although biological grafts have been utilized as a vascular conduit in leg bypass for many years, reports of a bovine carotid artery graft (BCAG) in lower extremity revascularization have been scarce. This study analyzed the outcome of lower leg bypass using BCAG. METHODS: A retrospective review of a prospectively collected database of all patients undergoing lower extremity bypass using BCAG from 2002 to 2017 was performed. Clinical outcomes including graft patency and limb salvage were evaluated...
August 17, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28819221/functional-validation-of-gpihbp1-and-identification-of-a-functional-mutation-in-gpihbp1-for-milk-fat-traits-in-dairy-cattle
#19
Jie Yang, Xuan Liu, Dan Wang, Chao Ning, Haifei Wang, Qin Zhang, Li Jiang
In a previous genome-wide association study (GWAS) on milk production traits in a Chinese Holstein population, we revealed that GPIHBP1 is a novel promising candidate gene for milk fat content traits. In this study, we performed over-expression and RNAi experiments on GPIHBP1 in bovine primary mammary epithelial cells. The results showed that the expression of several important milk fat-related genes (LPL, CD36, VLDLR, ACACA and FASN) increased or decreased when the expression of GPIHBP1 was up- or down-regulated...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818893/complete-genome-sequence-of-a-hobi-like-virus-isolated-from-a-nelore-cow-with-gastroenteric-disease-in-the-state-of-s%C3%A3-o-paulo-brazil
#20
Adriana Cortez, João Pessoa Araújo, Eduardo Furtado Flores, Márcio Garcia Ribeiro, Jane Megid, Antonio Carlos Paes, José Paes de Oliveira Filho, Leila Sabrina Ullmann, Camila Dantas Malossi, Marcos Bryan Heinemann
The Hobi-like virus presents antigenic and molecular differences in relation to bovine virus diarrhea virus 1 and 2. The description of the complete genome of the Hobi-like virus SV757/15, isolated from a Nelore cow with gastroenteric disease in Brazil, will help in understanding the evolution and diversity of pestiviruses.
August 17, 2017: Genome Announcements
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