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https://www.readbyqxmd.com/read/27921341/unexpected-effect-of-the-vehicle-grain-ethanol-of-homeopathic-fsh-on-the-in-vitro-survival-and-development-of-isolated-ovine-preantral-follicles
#1
Lartiza F Lima, Rebeca M P Rocha, Ana Beatriz G Duarte, Ivina R Brito, Gerlane M Silva, Giovanna Q Rodrigues, Diana C S Nunes-Pinheiro, Antônia D Sales, Arlindo A Moura, Matthew B Wheeler, Ana Paula R Rodrigues, Cláudio C Campello, José Ricardo Figueiredo
The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic - FSH 6cH and allopathic FSH - rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α-MEM(+) supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p < ...
December 5, 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27921109/dna-and-bsa-damage-inhibitory-activities-and-anti-acetylcholinesterase-anti-porcine-%C3%AE-amylase-and-antioxidant-properties-of-dolichos-lablab-beans
#2
Hosam M Habib, Serah W Theuri, Ehab Kheadr, Fedah E Mohamed
The underutilized Kenyan variety of Dolichos lablab bean seeds serve as a good source of natural antioxidants, which can probably be effective in reducing the risk of occurrence of several diseases. This study was undertaken for the first time to address the limited knowledge regarding the antioxidant activities of lablab beans. Moreover, their DNA damage inhibitory activity, bovine serum albumin (BSA) damage inhibitory activity, and the inhibition of acetylcholinesterase and porcine α-amylase were also investigated...
December 6, 2016: Food & Function
https://www.readbyqxmd.com/read/27921033/new-tools-to-study-contact-activation
#3
Steffen Rosén
The recent availability of a sensitive chromogenic method approach for determination of FXIa activity has been explored for designing sensitive methods for FXIIa and kallikrein, both using FXa formation as the read-out. For both enzymes the assay range 1-10 nmol/L provides a resolution of about 0.8 absorbance units with a total assay time of about 20 min. For studies on activation kinetics, subsampling and extensive dilution can be performed in MES-bovine serum albumin (BSA) buffer pH 5.7 for quenching of enzyme activity and with ensuing determination of FXa generation in a chromogenic FXIa method...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27920798/evolutionary-and-functional-features-of-copy-number-variation-in-the-cattle-genome
#4
Brittney N Keel, Amanda K Lindholm-Perry, Warren M Snelling
Genomic structural variations are an important source of genetic diversity. Copy number variations (CNVs), gains and losses of large regions of genomic sequence between individuals of a species, have been associated with a wide variety of phenotypic traits. However, in cattle, as well as many other species, relatively little is understood about CNV, including frequency of CNVs in the genome, sizes, and locations, chromosomal properties, and evolutionary processes acting to shape CNV. In this work, we focused on copy number variation in the bovine genome, with the aim to detect CNVs in Bos taurus coding sequence and explore potential evolutionary mechanisms shaping these CNV...
2016: Frontiers in Genetics
https://www.readbyqxmd.com/read/27920212/inhibition-of-antigen-specific-and-non-specific-stimulation-of-bovine-t-and-b-cells-by-lymphostatin-from-attaching-and-effacing-escherichia-coli
#5
Robin L Cassady-Cain, Elizabeth A Blackburn, Charlotte R Bell, Elizaveta Elshina, Jayne C Hope, Mark P Stevens
Enterohaemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) are enteric bacterial pathogens of worldwide importance. Most EPEC and non-O157 EHEC strains express lymphostatin (also known as LifA), a chromosomally-encoded 365 kDa protein. We previously demonstrated that lymphostatin is a putative glycosyltransferase that is important in intestinal colonisation of cattle by EHEC serogroup O5, O111 and O26 strains. However, the nature and consequences of the interaction between lymphostatin and immune cells from the bovine host are ill-defined...
December 5, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27919889/study-of-intracellular-staphylococcus-aureus-control-by-virulent-bacteriophage-within-mac-t-bovine-mammary-epithelial-cells
#6
Lili Zhang, Lichang Sun, Ruicheng Wei, Qiang Gao, Tao He, Cunfa Xu, Xianjin Liu, Ran Wang
Bacteriophages (phages) are known to effectively kill extracellular multiplying bacteria. The present study demonstrated that phages penetrated bovine mammary epithelial cells and cleared intracellular Staphylococcus aureus, in a time-dependent manner. In particular, phage vB_SauM_JS25 reached the nucleus within the 3 h post-incubation. The phages had an endocytotic efficiency of 12%. This ability to kill intracellular host bacteria suggests the utility of phage-based therapies, and may protect from recurrent infection and treatment failure...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27919607/a-novel-technique-for-bifurcated-bovine-plus-omniflow-aortic-graft-reconstruction
#7
P W Stather, A Q Howard
No abstract text is available yet for this article.
December 2, 2016: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/27919552/decoding-the-ecological-function-of-accessory-genome
#8
Michelle Qiu Carter
Shiga toxin-producing Escherichia coli O157:H7 primarily resides in cattle asymptomatically, and can be transmitted to humans through food. A study by Lupolova et al. applied a machine-learning approach to complex pan-genome information and predicted that only a small subset of bovine isolates have the potential to cause diseases in humans.
December 2, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27918933/tyrosine-fluorescence-probing-of-conformational-changes-in-tryptophan-lacking-domain-of-albumins
#9
N G Zhdanova, E G Maksimov, A M Arutyunyan, V V Fadeev, E A Shirshin
We addressed the possibility of using tyrosine (Tyr) fluorescence for monitoring conformational changes of proteins which are undetectable via tryptophan (Trp) fluorescence. The model objects, human (HSA) and bovine (BSA) serum albumins, contain one and two Trp residues, respectively, while Tyr is more uniformly distributed over their structure. The results of the investigation of albumins interaction with ethanol using intrinsic Trp and Tyr steady-state and time-resolved picosecond fluorescence indicated the presence of an intermediate at 10% (v/v) of ethanol in solution, that was supported by the results of extrinsic fluorescence measurements with the Nile Red dye...
November 25, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27918877/a-longitudinal-examination-of-peritraumatic-emotional-responses-and-their-association-with-posttraumatic-stress-disorder-and-major-depressive-disorder-among-veterans
#10
Eden Engel-Rebitzer, Michelle J Bovin, Shimrit K Black, Raymond C Rosen, Terence M Keane, Brian P Marx
Research has revealed a significant association between several peritraumatic emotional responses and posttraumatic stress disorder (PTSD). Preliminary research has also linked peritraumatic emotional responses with a diagnosis of major depressive disorder (MDD). The majority of this research has been cross-sectional, thereby making it difficult to determine the extent to which the various peritraumatic emotional responses may increase risk for, or serve as a premorbid marker of, PTSD and MDD. This study examined the longitudinal role of peritraumatic emotional responses on the subsequent development of PTSD and MDD in a sample of United States military veterans...
December 5, 2016: Journal of Trauma & Dissociation
https://www.readbyqxmd.com/read/27917821/optimizing-cell-sourcing-for-clinical-translation-of-tissue-engineered-ears
#11
Kerry A Morrison, Benjamin P Cohen, Ope Asanbe, Xue Dong, Alice Harper, Lawrence J Bonassar, Jason A Spector
: Background . Currently, the major impediment to clinical translation of our previously described platform for the fabrication of high fidelity, patient-specific tissue engineered ears is the development of a clinically optimal cell sourcing strategy. A limited autologous auricular chondrocyte (AuC) supply in conjunction with rapid chondrocyte de-differentiation during in vitro expansion currently makes clinical translation more challenging. Mesenchymal stem cells (MSCs) offer significant promise due to their inherent chondrogenic potential, and large availability through minimally invasive procedures...
December 5, 2016: Biofabrication
https://www.readbyqxmd.com/read/27917567/effects-of-rinsing-with-arginine-bicarbonate-and-urea-solutions-on-initial-enamel-lesions-in-situ
#12
Yang Yu, Xiaoling Wang, Chunling Ge, Bing Wang, Chuoyue Cheng, Ye-Hua Gan
OBJECTIVE: The aim of this study was to investigate the effects of rinsing with arginine or urea solution on initial enamel lesions in situ. METHODS: Fourteen subjects wore mandibular removable partial dentures embedded with bovine enamel blocks with artificial enamel lesions were included. The experiment included four 4-week rinsing periods with a 10-day washout period between each rinsing period. In each rinsing period, the subjects rinsed after meal or snack using water, or 2% arginine bicarbonate, or 1% urea, or 0...
December 5, 2016: Oral Diseases
https://www.readbyqxmd.com/read/27917511/evidence-for-direct-effects-of-glyphosate-on-ovarian-function-glyphosate-influences-steroidogenesis-and-proliferation-of-bovine-granulosa-but-not-theca-cells-in-vitro
#13
Maria Chiara Perego, Luis F Schutz, Francesca Caloni, Cristina Cortinovis, Marco Albonico, Leon J Spicer
Glyphosate (GLY) is a common herbicide used worldwide but its effect on ovarian function in mammals is unknown. The aim of this study was to determine the potential endocrine disruptor effects of GLY on ovarian function evaluating cell proliferation, steroidogenesis and gene expression using bovine granulosa cells (GC) and theca cells as in vitro models. GC proliferation was impaired (P < 0.05) after exposure to GLY at 0.5, 1.7 and 5 μg ml(-1) . GC progesterone production was not affected (P ≥ 0...
December 5, 2016: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/27917323/development-and-evaluation-of-a-novel-vaccine-against-prevalent-invasive-multi-drug-resistant-strains-of-streptococcus-pneumoniae
#14
Rehab H Bahy, Hayam M Hamouda, Amal S Shahat, Aymen S Yassin, Magdy A Amin
Streptococcus pneumoniae is a pathogen that causes serious invasive infections, such as septicemia, meningitis and pneumonia in addition to mild upper respiratory tract infections. Protection from pneumococcal diseases is thought to be mediated mainly by serotype-specific antibodies to capsular antigens. Pneumococcal conjugate vaccine consists of sugars (polysaccharides) from the capsule of the bacterium S. pneumoniae that are conjugated to a carrier protein. Three pneumococcal conjugated vaccines, each directed against a group of serotypes, are registered in Egypt; however, local vaccine production is required to cover the most prevalent serotypes...
2016: PeerJ
https://www.readbyqxmd.com/read/27916784/a-pcr-rflp-assay-to-detect-and-type-cytolethal-distending-toxin-cdt-genes-in-campylobacter-hyointestinalis
#15
Noritoshi Hatanaka, Kazumasa Kamei, Srinuan Somroop, Sharda Prasad Awasthi, Masahiro Asakura, Naoaki Misawa, Atsushi Hinenoya, Shinji Yamasaki
Campylobacter hyointestinalis is considered as an emerging zoonotic pathogen. We have recently identified two types of cytolethal distending toxin (cdt) gene in C. hyointestinalis and designated them as Chcdt-I and Chcdt-II. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay that can differentiate Chcdt-I from Chcdt-II. When the PCR-RFLP assay was applied to 17 other Campylobacter strains and 25 non-Campylobacter strains, PCR products were not obtained irrespective of their cdt gene-possession, indicating that the specificity of the PCR-RFLP assay was 100%...
December 4, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27916667/development-and-validation-of-an-immunohistochemistry-procedure-for-the-detection-of-a-neurotropic-bovine-astrovirus
#16
Céline L Boujon, Senija Selimovic-Hamza, Ilias Bouzalas, Torsten Seuberlich
Members of the Astroviridae family are best known to cause diarrhea in different mammalian species. Lately, some strains have been associated with encephalitis in humans, minks and cattle. In this study, we developed an immunohistochemistry (IHC) procedure for the detection of a neurotropic bovine astrovirus (BoAstV-CH13/NeuroS1), which is associated with non-suppurative encephalitis in cattle. We expressed five recombinant antigens corresponding to different putative viral proteins of BoAstV-CH13/NeuroS1. Antigens were then used for the production of hyperimmune sera in rabbits...
January 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/27916572/high-resolution-melting-hrm-for-genotyping-bovine-ephemeral-fever-virus-befv
#17
Oran Erster, Rotem Stram, Shopia Menasherow, Marisol Rubistein-Giuni, Binyamin Sharir, Evgeni Kchinich, Yehuda Stram
In recent years there have been several major outbreaks of bovine ephemeral disease in the Middle East, including Israel. Such occurrences raise the need for quick identification of the viruses responsible for the outbreaks, in order to rapidly identify the entry of viruses that do not belong to the Middle-East BEFV lineage. This challenge was met by the development of a high-resolution method (HRM) assay. The assay is based on the viral G gene sequence and generation of an algorithm that calculates and evaluates the GC content of various fragments...
December 1, 2016: Virus Research
https://www.readbyqxmd.com/read/27916114/competitive-inhibition-assay-for-the-detection-of-progesterone-in-dairy-milk-using-a-fiber-optic-spr-biosensor
#18
D Daems, J Lu, F Delport, N Mariën, L Orbie, B Aernouts, I Adriaens, T Huybrechts, W Saeys, D Spasic, J Lammertyn
Analytical methods that are often used for the quantification of progesterone in bovine milk include immunoassays and chromatographic techniques. Depending on the selected method, the main disadvantages are the cost, time-to-result, labor intensity and usability as an automated at-line device. This paper reports for the first time on a robust and practical method to quantify small molecules, such as progesterone, in complex biological samples using an automated fiber optic surface plasmon resonance (FO-SPR) biosensor...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27915587/the-determination-of-testosterone-esters-and-estradiol-esters-in-bovine-and-porcine-blood-serum
#19
Martina Rejtharová, Libor Rejthar, Katarína Čačková
Monitoring of steroid esters in blood serum is desirable in order to detect possible illegal use of natural hormones as growth promoters. A method for determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate in bovine and porcine blood serum was developed. The procedure consists of protein precipitation and removal of phospholipids using a HybridSPE®-Phospholipid column followed by clean-up on a hydrophilic modified styrene polymer Supel(TM) -Select HLB column and LC-MS/MS measurement...
December 5, 2016: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/27915439/presence-of-ureaplasma-diversum-in-the-genital-tracts-of-female-dairy-cattle-in-mato-grosso-state-brazil
#20
Jaqueline B Azevedo, Gustavo S Silva, Priscylla S Rocha, Letícia C Pitchenin, Valéria Dutra, Luciano Nakazato, Anderson Castro Soares de Oliveira, Caroline A Pescador
Ureaplasma diversum infection in bovine females may result in various reproductive problems, including granular vulvovaginitis, abortion, weak calves, salpingitis, and spontaneous abortion. The presence of U. diversum in a dairy bovine population from midwestern Brazil has not been established. The aim of this study was to determine whether U. diversum was present in dairy cattle from midwestern Brazil using polymerase chain reaction (PCR). Vulvovaginal mucus was analyzed from 203 cows located in six municipalities in the north region of Mato Grosso State, Brazil...
December 3, 2016: Tropical Animal Health and Production
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