keyword
MENU ▼
Read by QxMD icon Read
search

hTERT

keyword
https://www.readbyqxmd.com/read/28544703/high-ubiquitin-specific-protease-44-expression-induces-dna-aneuploidy-and-provides-independent-prognostic-information-in-gastric-cancer
#1
Sho Nishimura, Eiji Oki, Koji Ando, Makoto Iimori, Yu Nakaji, Yuichiro Nakashima, Hiroshi Saeki, Yoshinao Oda, Yoshihiko Maehara
Chromosomal instability (CIN), characterized by aneuploidy, is a major molecular subtype of gastric cancer. The deubiquitinase USP44 is an important regulator of APC activation in the spindle checkpoint and leads to proper chromosome separation to prevent aneuploidy. Aberrant expression of USP44 leads CIN in cells; however, the correlation between USP44 and DNA aneuploidy in gastric cancer is largely unknown. We analyzed USP44 expression in 207 patients with gastric cancer by immunohistochemistry and found that the proportion of USP44 expression was higher in gastric cancer tumors (mean, 39...
May 23, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28535013/htert-c250t-promoter-mutation-and-telomere-length-as-a-molecular-markers-of-cancer-progression-in-patients-with-head-and-neck-cancer
#2
Wojciech Barczak, Wiktoria M Suchorska, Agnieszka Sobecka, Karolina Bednarowicz, Piotr Machczynski, Pawel Golusinski, Blazej Rubis, Michal M Masternak, Wojciech Golusinski
Squamous cell carcinoma of the head and neck (HNSCC) is the sixth leading cause of cancer worldwide, representing over half a million incidents every year. Cancer cells, including HNSCC, are characterized by increased telomerase activity. This enzymatic complex is active in ~90% of all cancer types and is responsible for the lengthening of telomeres. Highly recurrent point mutations in the human telomerase reverse transcriptase (hTERT) promoter have recently been reported in a number of human neoplasms. The aim of the present study was to analyze the prevalence of the hTERT promoter C250T mutation and telomere length in the blood leukocytes of 61 patients with HNSCC and 49 healthy individuals...
May 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28529064/analysis-of-post-transcriptional-regulation-during-cancer-progression-using-a-donor-derived-isogenic-model-of-tumorigenesis
#3
Laura S Bisogno, Jack D Keene
Post-transcriptional regulation of gene expression by RNA binding proteins (RBPs) and non-coding RNAs plays an important role in global gene expression. Many post-transcriptional regulators are misexpressed and misregulated in cancers, resulting in altered programs of protein biosynthesis that can drive tumor progression. While comparative studies of several RBPs and microRNAs expressed in various cancer types have been reported, a model system that can be used to quantify RBP regulation and functional outcomes during the initiation and early stages of tumorigenesis is lacking...
May 18, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28514770/mm-bmscs-induce-na%C3%A3-ve-cd4-t-lymphocytes-dysfunction-through-fibroblast-activation-protein-%C3%AE
#4
Xiaofei Wu, Yadan Wang, Jian Xu, Ting Luo, Jun Deng, Yu Hu
BACKGROUND: The tumor microenvironment plays a major role in multiple myelomas (MM). MM-BMSCs (bone marrow mesenchymal stromal cells) can support tumor growth and immune surveillance escape. On the other hand, fibroblast activation protein α, expressed by cancer stroma cells including BMSCs, has been shown to potentiate epithelial cancers growth and immune suppression. RESULTS: MM-BMSC inhibited proliferation of T cells (P = 0.0138), promoted senescence of T cells (P < 0...
April 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28513547/epigenetic-regulation-of-telomere-maintenance-for-therapeutic-interventions-in-gliomas
#5
REVIEW
Elisabeth Naderlinger, Klaus Holzmann
High-grade astrocytoma of WHO grade 4 termed glioblastoma multiforme (GBM) is a common human brain tumor with poor patient outcome. Astrocytoma demonstrates two known telomere maintenance mechanisms (TMMs) based on telomerase activity (TA) and on alternative lengthening of telomeres (ALT). ALT is associated with lower tumor grades and better outcome. In contrast to ALT, regulation of TA in tumors by direct mutation and epigenetic activation of the hTERT promoter is well established. Here, we summarize the genetic background of TMMs in non-malignant cells and in cancer, in addition to clinical and pathological features of gliomas...
May 17, 2017: Genes
https://www.readbyqxmd.com/read/28503498/evaluation-of-energy-balance-on-human-telomerase-reverse-transcriptase-htert-alternative-splicing-by-semi-quantitative-rt-pcr-in-human-umbilical-vein-endothelial-cells
#6
Mohaddeseh Behjati, Mohammad Hashemi, Mohammad Kazemi, Mansoor Salehi, Shaghayegh Haghjooy Javanmard
BACKGROUND: Decreased high-energy phosphate level is involved in endothelial cell injury and dysfunction. Reduced telomerase activity in endothelial cells in parallel with reduced energy levels might be due to altered direction of alternative splicing machine as a complication of depleted energy during the process of atherosclerosis. MATERIALS AND METHODS: Isolated human umbilical vein endothelial cells (HUVECs) were treated for 24 hours by oligomycine (OM) and 2-deoxy glucose (2-DG)...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28492540/nmi-inhibits-cancer-stem-cell-traits-by-downregulating-htert-in-breast-cancer
#7
Xu Feng, Xiangdong Xu, Xiangsheng Xiao, Kun Zou, Wendan Yu, Jiali Wu, Ranran Tang, Yue Gao, Jiaojiao Hao, Xinrui Zhao, Yina Liao, Yiming Chen, Wenlin Huang, Wei Guo, Lan Kang, Wuguo Deng
N-myc and STAT interactor (NMI) has been proved to bind to different transcription factors to regulate a variety of signaling mechanisms including DNA damage, cell cycle and epithelial-mesenchymal transition. However, the role of NMI in the regulation of cancer stem cells (CSCs) remains poorly understood. In this study, we investigated the regulation of NMI on CSCs traits in breast cancer and uncovered the underlying molecular mechanisms. We found that NMI was lowly expressed in breast cancer stem cells (BCSCs)-enriched populations...
May 11, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28492516/circulating-cell-free-dna-and-circulating-tumor-cells-as-prognostic-and-predictive-biomarkers-in-advanced-non-small-cell-lung-cancer-patients-treated-with-first-line-chemotherapy
#8
Simona Coco, Angela Alama, Irene Vanni, Vincenzo Fontana, Carlo Genova, Maria Giovanna Dal Bello, Anna Truini, Erika Rijavec, Federica Biello, Claudio Sini, Giovanni Burrafato, Claudia Maggioni, Giulia Barletta, Francesco Grossi
Cell-free DNA (cfDNA) and circulating tumor cells (CTCs) are promising prognostic and predictive biomarkers in non-small cell lung cancer (NSCLC). In this study, we examined the prognostic role of cfDNA and CTCs, in separate and joint analyses, in NSCLC patients receiving first line chemotherapy. Seventy-three patients with advanced NSCLC were enrolled in this study. CfDNA and CTC were analyzed at baseline and after two cycles of chemotherapy. Plasma cfDNA quantification was performed by quantitative PCR (qPCR) whereas CTCs were isolated by the ScreenCell Cyto (ScreenCell, Paris, France) device and enumerated according to malignant features...
May 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28485781/effects-of-htert-antisense-oligodeoxynucleotide-on-cell-apoptosis-and-expression-of-htert-and-bcl-2-mrna-in-keloid-fibroblasts
#9
Y Huang, L-X Lin, Q-X Bi, P Wang, X-M Wang, J Liu, Y-T Wang
OBJECTIVE: This study was purposed to investigate the effects of hTERT antisense oligodeoxynucleotide (ASODN) on cell apoptosis and expression of hTERT and bcl-2 mRNA in keloid fibroblasts and to explore its anti-keloid effect. MATERIALS AND METHODS: Primary cultures of dermal fibroblasts derived from 12 keloid samples were established, strains of fibroblasts at passages 3 to 4 were used in this study. After treated by hTERT ASODN the proliferation of the fibroblasts was measured by cell count and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay method, the apoptosis was analyzed by flow cytometry (FCM), and the expression of hTERT and bcl-2 mRNA were observed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR)...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28479277/selectivity-of-major-isoquinoline-alkaloids-from-chelidomium-majus-towards-telomeric-g-quadruplex-a-study-using-a-modified-transition-fret-t-fret-assay
#10
Sakineh Kazemi Noureini, Hosein Esmaeili, Farzane Abachi, Soraia Khiali, Barira Islam, Martyna Kuta, Ali A Saboury, Marcin Hoffmann, Jiri Sponer, Gary Parkinson, Shozeb Haider
BACKGROUND: Natural bioproducts are invaluable resources in drug discovery. Isoquinoline alkaloids of Chelidonium majus constitute a structurally diverse family of natural products that are of great interest, one of them being their selectivity for human telomeric G-quadruplex structure and telomerase inhibition. METHODS: The study focuses on the mechanism of telomerase inhibition by stabilization of telomeric G-quadruplex structures by berberine, chelerythrine, chelidonine, sanguinarine and papaverine...
May 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28472785/cdc5l-promotes-htert-expression-and-colorectal-tumor-growth
#11
Jia Li, Ningning Zhang, Rui Zhang, Longmei Sun, Wendan Yu, Wei Guo, Yingying Gao, Mei Li, Wei Liu, Pin Liang, Wuguo Deng, Xiaonan Cui
BACKGROUND/AIMS: Colorectal cancer (CRC) is the third leading cause of cancer-related death worldwide because the survival rate remains low. Cell division cycle 5-like (CDC5L) is highly expressed in some cancer cells, but the mechanism requires clarification. Human telomerase reverse transcriptase (hTERT) plays important roles in CRC. METHODS: This study aimed to identify a link between CDC5L and hTERT and to determine their effects on the signaling pathways, migration and prognosis of CRC cells...
May 4, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28460480/htert-promotes-gastric-intestinal-metaplasia-by-upregulating-cdx2-via-nf-%C3%AE%C2%BAb-signaling-pathway
#12
Bai-Jun Chen, Shuo Zeng, Rui Xie, Chang-Jiang Hu, Su-Ming Wang, Yu-Yun Wu, Yu-Feng Xiao, Shi-Ming Yang
BACKGROUND: hTERT has been reported involved in the proliferation and metastasis of gastric cancer, but the role of hTERT in gastric intestinal metaplasia, a premalignant lesion of the gastric mucosa was unknown. The aim of the present study was to investigate the role of hTERT in GIM and the effect of hTERT on CDX2 expression in gastric cells. RESULTS: Experiments showed that expression of hTERT was significantly higher in GIM than in normal gastric mucosa. Moreover, hTERT increased the KLF4 level via NF-κB during GIM...
April 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28455966/arginine-deiminase-expressed-in-vivo-driven-by-human-telomerase-reverse-transcriptase-promoter-displays-high-hepatoma-targeting-and-oncolytic-efficiency
#13
Hui Jiang, Song Guo, Dan Xiao, Xuzhao Bian, Jie Wang, Ying Wang, Huiting Zhou, Jun Cai, Zhongliang Zheng
Arginine starvation has the potential to selectively treat both primary tumor and (micro) metastatic tissue with very low side effects. Arginine deiminase (ADI; EC 3.5.3.6), an arginine-degrading enzyme, has been studied as a potential anti-tumor drug for the treatment of arginine-auxotrophic tumors. Though ADI-PEG20 (pegylated ADI by PEG 20,000) already passed the phase I/II clinical trials [1], it is just used as adjuvant therapy because of its low efficiency and less targeting. Then, this paper discussed the efficiency of arginine starvation mediated by ADI expressed in cytoplasm for liver cancers...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28454693/cytogenetic-features-of-human-trophoblast-cell-lines-swan-71-and-3a-sube
#14
Jill L Reiter, Holli M Drendel, Sujata Chakraborty, Megan M Schellinger, Men-Jean Lee, Gil Mor
Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability...
April 2017: Placenta
https://www.readbyqxmd.com/read/28454681/human-mesenchymal-stem-cells-with-enhanced-telomerase-activity-acquire-resistance-against-oxidative-stress-induced-genomic-damage
#15
Varvara Trachana, Spyros Petrakis, Zisis Fotiadis, Evangelia K Siska, Vasileios Balis, Efstathios S Gonos, Martha Kaloyianni, George Koliakos
BACKGROUND: Human mesenchymal stem cells (MSC) are important tools for several cell-based therapies. However, their use in such therapies requires in vitro expansion during which MSCs quickly reach replicative senescence. Replicative senescence has been linked to macromolecular damage, and especially oxidative stress-induced DNA damage. Recent studies on the other hand, have implicated telomerase in the cellular response to oxidative damage, suggesting that telomerase has a telomere-length independent function that promotes survival...
April 25, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28450567/circulating-tumor-dna-reflects-tumor-metabolism-rather-than-tumor-burden-in-chemotherapy-naive-patients-with-advanced-non-small-cell-lung-cancer-nsclc-an-18-f-fdg-pet-ct-study
#16
Silvia Morbelli, Angela Alama, Giulia Ferrarazzo, Simona Coco, Carlo Genova, Erica Rijavec, Francesca Bongioanni, Federica Biello, Maria Giovanna Dal Bello, Giulia Barletta, Michela Massollo, Irene Vanni, Roberta Piva, Alberto Nieri, Matteo Bauckneht, Gianmario Sambuceti, Francesco Grossi
We aimed to evaluate the relationship between Circulating tumor Cells (CTCs) and plasma Cell-free DNA (cfDNA) on one side and a comprehensive range of (18)F-fluorodeoxyglucose (FDG)-PET/CT-derived parameters on the other side in chemotherapy-naïve patients with advanced NSCLC. Methods: Among the seventy-nine patients included in the VeriStrat® trial (evaluating the role of pretreatment circulating tumor markers as predictors of prognosis), we selected patients submitted to FDG-PET/CT for clinical reasons just before the inclusion in the trial...
April 27, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28447256/establishment-of-a-protocol-to-extend-the-lifespan-of-human-hormone-secreting-pituitary-adenoma-cells
#17
Aurora Aiello, Maria Francesca Cassarino, Simona Nanni, Antonella Sesta, Francesco Ferraú, Claudio Grassi, Marco Losa, Francesco Trimarchi, Alfredo Pontecorvi, Salvatore Cannavò, Francesca Pecori Giraldi, Antonella Farsetti
PURPOSE: The aim of this study was to generate immortalized human anterior pituitary adenoma cells. Reliable cell models for the study of human pituitary adenomas are as yet lacking and studies performed so far used repeated passaging of freshly excised adenomas, with the attendant limitations due to limited survival in culture, early senescence, and poor reproducibility. METHODS & RESULTS: We devised a technique based upon repeated co-transfections of two retroviral vectors, one carrying the catalytic subunit of human telomerase, hTERT, the other SV40 large T antigen...
April 26, 2017: Endocrine
https://www.readbyqxmd.com/read/28443573/the-normal-and-tumour-tissue-mrna-expressions-of-telomerase-complex-genes-in-several-types-of-cancer
#18
Emel Çalışkan Can, M Can Atalay, Ece Miser Salihoğlu, Ülkü Yalçıntaş Arslan, H Bolkan Şimşek, Sevgi Yardım Akaydın
AIMS: The aim of this study is to investigate the changes in mRNA expression levels of telomerase related significant proteins in several types of cancer. METHODS: Human telomerase reverse transcriptase (hTERT), pontin, reptin and dyskerin expressions were measured in normal and tumour tissues obtained from 26 patients with colorectal, breast, and gastric cancers, using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) method. RESULTS: For all the patients, no significant difference was found in mRNA expressions of hTERT and dyskerin (p>0...
April 13, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28443470/a-multi-target-protein-of-htertr-fam96a-presents-significant-anticancer-potent-in-the-treatment-of-hepatocellular-carcinoma
#19
Meng-Yu Zhang, Jie-Ping Wang
The abilities to escape apoptosis induced by anticancer drugs are an essential factor of carcinogenesis and a hallmark of resistance to cancer therapy. In this study, we identified hTERTR-FAM96A (human telomerase reverse transcriptase-family with sequence similarity 96 member A) as a new efficient agent for apoptosome-activating and anti-tumor protein and investigated the potential tumor suppressor function in hepatocellular carcinoma. The hTERTR-FAM96A fusion protein was constructed by genetic engineering and its anticancer function of hTERTR-FAM96A was explored in vitro and in vivo by investigating the possible preclinical outcomes...
April 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28435520/genetically-transforming-human-osteoblasts-to-sarcoma-development-of-an-osteosarcoma-model
#20
Yi Yang, Rui Yang, Michael Roth, Sajida Piperdi, Wendong Zhang, Howard Dorfman, Pulivarthi Rao, Amy Park, Sandeep Tripathi, Carrie Freeman, Yunjia Zhang, Rebecca Sowers, Jeremy Rosenblum, David Geller, Bang Hoang, Jonathan Gill, Richard Gorlick
Osteosarcoma is the most common primary malignant bone tumor in children and young adults. Although histologically defined by the presence of malignant osteoid, the tumor possesses lineage multipotency suggesting it could be derived from a cell anywhere on the differentiation pathway between a mesenchymal stem cell (MSC) and a mature osteoblast. To determine if preosteoblasts (pOB) could be the cell of origin differentiated MSCs were transformed with defined genetic elements. MSCs and pOB differentiated from the same MSCs were serially transformed with the oncogenes hTERT, SV40 large T antigen and H-Ras...
January 2017: Genes & Cancer
keyword
keyword
30169
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"