keyword
MENU ▼
Read by QxMD icon Read
search

cAMP

keyword
https://www.readbyqxmd.com/read/28231640/mind-the-gap-mechanisms-regulating-the-endothelial-barrier
#1
REVIEW
Mariya Y Radeva, Jens Waschke
The endothelial barrier consists of intercellular contacts localized in the cleft between endothelial cells, which is covered by the glycocalyx in a sieve-like manner. Both types of barrier-forming junctions, i.e. the adherens junction (AJ) serving mechanical anchorage and mechanotransduction and the tight junction (TJ) sealing the intercellular space to limit paracelullar permeability, are tethered to the actin cytoskeleton. Under resting conditions, the endothelium thereby builds a selective layer controlling the exchange of fluid and solutes with the surrounding tissue...
February 23, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28231251/empirical-lessons-regarding-contraception-in-a-protracted-refugee-setting-a-descriptive-study-from-maela-camp-on-the-thai-myanmar-border-1996-2015
#2
Somjet Srikanok, Daniel M Parker, Amber L Parker, Tracey Lee, Aung Myat Min, Pranee Ontuwong, Saw Oo Tan, Supachai Sirinonthachai, Rose McGready
Conflict settings and refugee camps can be chaotic places, with large and rapid population movements, exacerbated public health problems, and ad hoc health services. Reproductive health care that includes family planning is of heightened importance in such settings, however, funding and resources tend to be constrained and geared towards acute health services such as trauma management and infectious disease containment. Here we report on the complexities and challenges of providing family planning in a post-emergency refugee setting, using the example of the largest refugee camp on the Thai-Myanmar border, in existence now for over 30 years...
2017: PloS One
https://www.readbyqxmd.com/read/28228966/objective-image-analysis-of-real-time-three-dimensional-intraoperative-ultrasound-for-intrinsic-brain-tumour-surgery
#3
Sophie J Camp, Vasileios Apostolopoulos, Vasileios Raptopoulos, Amrish Mehta, Kevin O'Neill, Mohammed Awad, Babar Vaqas, David Peterson, Federico Roncaroli, Dipankar Nandi
BACKGROUND: There is growing evidence that maximal surgical resection of primary intrinsic brain tumours is beneficial, both by improving progression free and overall survival and also by facilitating postoperative chemotherapy and radiotherapy. Hence, there has been an increase in the popularity of real-time intraoperative imaging in brain tumour surgery. The complex theatre arrangements, prohibitive cost and prolonged theatre time of intraoperative MRI have restricted its application...
2017: Journal of Therapeutic Ultrasound
https://www.readbyqxmd.com/read/28228615/levels-of-cyclic-amp-and-cyclic-gmp-in-porcine-oocyte-cumulus-complexes-and-cumulus-free-oocytes-derived-from-small-and-middle-follicles-during-the-first-24-hour-period-of-in-vitro-maturation
#4
Yuichi Okudaira, Takuya Wakai, Hiroaki Funahashi
The objective of this study was to compare the cAMP and cGMP levels in cumulus-oocyte complexes (COCs) derived from the middle follicles (MFs, 3-6 mm in diameter) and small follicles (SFs, 1-3 mm in diameter) of pre-pubertal gilts during the first 24-h period of maturation in vitro (IVM). Both cAMP and cGMP levels in MF- and SF-derived oocytes did not change during this period. Although the cAMP levels increased in the COCs at 10 and 20 h after the start of IVM, the levels of cAMP were significantly higher in MF-derived COCs than in SF-derived COCs at 20 h after the start of IVM...
February 23, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28228611/anti-diabetic-action-of-all-trans-retinoic-acid-and-the-orphan-g-protein-coupled-receptor-gprc5c-in-pancreatic-%C3%AE-cells
#5
Stefan Amisten, Israa Mohammad Al-Amily, Arvind Soni, Ross Hawkes, Patricio Atanes, Shanta Jean Persaud, Patrik Rorsman, Albert Salehi
Pancreatic islets express high levels of the orphan G-protein coupled receptor C5C (GPRC5C), the function of which remains to be established. Here we have examined the role of GPRC5C in the regulation of insulin secretion and β-cell survival and proliferation using human and mouse pancreatic islets. The expression of GPRC5C was analysed by RNA-sequencing, qPCR, western blotting and confocal microscopy. Insulin secretion and cell viability were determined by RIA and MTS assays, respectively. GPRC5C mRNA expression and protein level were reduced in the islets from type-2 diabetic donors...
February 22, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28228402/aliskiren-increases-aquaporin-2-expression-and-attenuates-lithium-induced-nephrogenic-diabetes-insipidus
#6
Yu Lin, Tiezheng Zhang, Pinning Feng, Miaojuan Qiu, Qiaojuan Liu, Suchun Li, Peili Zheng, Yonglun Kong, Moshe Levi, Chunling Li, Weidong Wang
The direct renin inhibitor aliskiren has been shown to retain and persist in medullary collecting ducts even after treatment was discontinued, suggesting a new mechanism of action for this drug. The purpose of the present study was to investigate whether aliskiren regulates renal aquaporin expression in the collecting ducts and improves urinary concentrating defect induced by lithium in mice. The mice were either fed with normal chow or LiCl diet (40mM/kg dry food/day for 4 days and 20mM/kg dry food/day for last 3 days) for seven days...
February 22, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28228354/effect-of-tamoxifen-on-spermatogenesis-and-testicular-steroidogenesis
#7
Rachna Verma, Amitabh Krishna
The aim of this study was to evaluate the effects of in vivo and in vitro treatments with selective estrogen receptor modulator (SERM), tamoxifen on testicular functions. The testis treated with tamoxifen, in vivo or in vitro, showed dose-dependent regressive changes in spermatogenesis. This study showed that the decreased estrogenic effect due to tamoxifen may be directly responsible for decreased testicular expression of aromatase, which in turn may be responsible for decreased synthesis of estradiol in the testis...
February 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28225778/gnb3-and-creb1-gene-polymorphisms-combined-with-negative-life-events-increase-susceptibility-to-major-depression-in-a-chinese-han-population
#8
Jingsong Ma, Lin Wang, Yanjie Yang, Zhengxue Qiao, Deyu Fang, Xiaohui Qiu, Xiuxian Yang, Xiongzhao Zhu, Jincai He, Hui Pan, Bo Ban, Yan Zhao, Hong Sui
BACKGROUND: Major depression (MD) is caused by a combination of genetic and environmental factors. In this study we investigated the interaction of variations in the G-protein beta 3 subunit (GNB3) and cAMP response element binding protein 1 (CREB1) genes with negative life events in the pathogenesis of MD. One GNB3 polymorphism (rs5443) and four CREB1 polymorphisms (rs2253206, rs2551941, rs6740584, rs11904814) were investigated based on known associations with MD. METHODS: 512 patients with MD and 513 control subjects were genotyped...
2017: PloS One
https://www.readbyqxmd.com/read/28225069/testicular-dnmt3-expression-and-global-dna-methylation-are-down-regulated-by-gonadotropin-releasing-hormones-in-the-ricefield-eel-monopterus-albus
#9
Yize Zhang, Xin Sun, Lihong Zhang, Weimin Zhang
In vertebrates, DNA methyltransferase 3 (Dnmt3) homologues are responsible for de novo DNA methylation and play important roles in germ cell development. In the present study, four dnmt3 genes, dnmt3aa, dnmt3ab, dnmt3ba and dnmt3bb.1, were identified in ricefield eels. Real-time quantitative PCR analysis showed that all four dnmt3 mRNAs were detected broadly in tissues examined, with testicular expression at relatively high levels. In the testis, immunostaining for all four Dnmt3 forms was mainly localized to spermatocytes, which also contained highly methylated DNA...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28223430/young-adult-and-usual-adult-body-mass-index-and-multiple-myeloma-risk-a-pooled-analysis-in-the-international-multiple-myeloma-consortium-immc
#10
Brenda M Birmann, Gabriella Andreotti, Anneclaire J De Roos, Nicola J Camp, Brian C H Chiu, John J Spinelli, Nikolaus Becker, Veronique Benhaim-Luzon, Parveen Bhatti, Paolo Boffetta, Paul Brennan, Elizabeth E Brown, Pierluigi Cocco, Laura Costas, Wendy Cozen, Silvia de Sanjosé, Lenka Foretová, Graham G Giles, Marc Maynadié, Kirsten B Moysich, Alexandra Nieters, Anthony Staines, Guido Tricot, Dennis Weisenburger, Yawei Zhang, Dalsu Baris, Mark P Purdue
BACKGROUND: Multiple myeloma (MM) risk increases with higher adult body mass index (BMI). Emerging evidence also supports an association of young adult BMI with MM. We undertook a pooled analysis of eight case-control studies to further evaluate anthropometric MM risk factors, including young adult BMI. METHODS: We conducted multivariable logistic regression analysis of usual adult anthropometric measures of 2,318 MM cases and 9,609 controls, and of young adult BMI (age 25 or 30 years) for 1,164 cases and 3,629 controls...
February 21, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28223412/identification-of-a-selective-small-molecule-inhibitor-of-type-1-adenylyl-cyclase-activity-with-analgesic-properties
#11
Tarsis F Brust, Doungkamol Alongkronrusmee, Monica Soto-Velasquez, Tanya A Baldwin, Zhishi Ye, Mingji Dai, Carmen W Dessauer, Richard M van Rijn, Val J Watts
Adenylyl cyclase 1 (AC1) belongs to a group of adenylyl cyclases (ACs) that are stimulated by calcium in a calmodulin-dependent manner. Studies with AC1 knockout mice suggest that inhibitors of AC1 may be useful for treating pain and opioid dependence. However, nonselective inhibition of AC isoforms could result in substantial adverse effects. We used chemical library screening to identify a selective AC1 inhibitor with a chromone core structure that may represent a new analgesic agent. After demonstrating that the compound (ST034307) inhibited Ca(2+)-stimulated adenosine 3',5'-monophosphate (cAMP) accumulation in human embryonic kidney (HEK) cells stably transfected with AC1 (HEK-AC1 cells), we confirmed selectivity for AC1 by testing against all isoforms of membrane-bound ACs...
February 21, 2017: Science Signaling
https://www.readbyqxmd.com/read/28223387/activity-of-sanguinarine-against-candida-albicans-biofilms
#12
Hua Zhong, Dan-Dan Hu, Gan-Hai Hu, Juan Su, Shuang Bi, Zhuo-Er Zhang, Zheng Wang, Ri-Li Zhang, Zheng Xu, Yuan-Ying Jiang, Yan Wang
C. albicans biofilms show resistance to many clinical antifungal agents, and play a considerable contributing role in the process of C. albicans infections. New antifungal agents against C. albicans biofilms are sorely needed. The aim of this study was to evaluate sanguinarine (SAN) for its activity against Candida albicans biofilms and explore the underlying mechanism. The MIC50 of SAN was 3.2 μg/mL. While ≥ 0.8 μg/mL SAN could suppress C. albicans biofilms. Further mechanism study revealed that ≥ 0...
February 21, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28223045/crystal-structures-reveal-a-new-and-novel-foxo1-binding-site-within-the-human-glucose-6-phosphatase-catalytic-subunit-1-gene-promoter
#13
Puja Singh, Eun Hee Han, James A Endrizzi, Richard M O'Brien, Young-In Chi
Human glucose-6-phosphatase plays a vital role in blood glucose homeostasis and holds promise as a therapeutic target for diabetes. Expression of its catalytic subunit gene 1 (G6PC1) is tightly regulated by metabolic-response transcription factors such as FoxO1 and CREB. Although at least three potential FoxO1 binding sites (insulin response elements, IREs) and one CREB binding site (cAMP response element, CRE) within the proximal region of the G6PC1 promoter have been identified, the interplay between FoxO1 and CREB and between FoxO1 bound at multiple IREs has not been well characterized...
February 18, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28222740/dibutyryl-camp-attenuates-pulmonary-fibrosis-by-blocking-myofibroblast-differentiation-via-pka-creb-cbp-signaling-in-rats-with-silicosis
#14
Yan Liu, Hong Xu, Yucong Geng, Dingjie Xu, Lijuan Zhang, Yi Yang, Zhongqiu Wei, Bonan Zhang, Shifeng Li, Xuemin Gao, Ruimin Wang, Xianghong Zhang, Darrell Brann, Fang Yang
BACKGROUND: Myofibroblasts play a major role in the synthesis of extracellular matrix (ECM) and the stimulation of these cells is thought to play an important role in the development of silicosis. The present study was undertaken to investigate the anti-fibrotic effects of dibutyryl-cAMP (db-cAMP) on rats induced by silica. METHODS: A HOPE MED 8050 exposure control apparatus was used to create the silicosis model. Rats were randomly divided into 4 groups: 1)controls for 16 w; 2)silicosis for 16 w; 3)db-cAMP pre-treatment; 4) db-cAMP post-treatment...
February 21, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28222489/characterization-of-a-novel-signal-transducer-element-intrinsic-to-class-iiia-b-adenylate-cyclases-and-guanylate-cyclases
#15
Miriam Ziegler, Jens Bassler, Stephanie Beltz, Anita Schultz, Andrei N Lupas, Joachim E Schultz
Adenylate cyclases (ACs) are signalling proteins that produce the second messenger cAMP. Class III ACs comprises four groups (class IIIa-d); of these, class IIIa and IIIb ACs have been identified in bacteria and eukaryotes. Many class IIIa ACs are anchored to membranes via hexahelical domains. In eukaryotic ACs, membrane anchors are well conserved, suggesting that this region possesses important functional characteristics that are as yet unknown. To address this question, we replaced the hexahelical membrane anchor of the mycobacterial AC Rv1625c by the hexahelical quorum-sensing receptor from Legionella, LqsS...
February 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28221775/electrostatic-interactions-as-mediators-in-the-allosteric-activation-of-pka-ri%C3%AE
#16
Emilia Pecora de Barros, Robert D Malmstrom, Kimya Nourbakhsh, Jason Cruz Del Rio, Alexandr P Kornev, Susan S Taylor, Rommie E Amaro
Close-range electrostatic interactions that form salt bridges are key components of protein stability. Here we investigate the role of these charged interactions in modulating the allosteric activation of Protein Kinase A (PKA) via computational and experimental mutational studies on a conserved basic patch located in the regulatory subunit's B/C helix. Molecular dynamics simulations evidenced the presence of an extended network of fluctuating salt bridges spanning the helix and connecting the two cAMP binding domains in its extremities...
February 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28219934/gja1-acts-downstream-of-acvr1-to-regulate-uterine-decidualization-via-hand2
#17
Hai-Fan Yu, Zhan-Peng Yue, Kai Wang, Zhan-Qing Yang, Hong-Liang Zhang, Shuang Geng, Bin Guo
Although Gja1 has been proved to play an important role in uterine decidualization, its regulatory mechanism remains largely unknown. Here, we showed that Gja1 was highly expressed in the decidual cells and promoted the proliferation of uterine stromal cells and expression of Prl8a2 and Prl3c1 which were two well-known differentiation markers for decidualization. Further analysis revealed that Gja1 might act downstream of Acvr1 and cAMP to regulate the differentiation of uterine stromal cells. Administration of cAMP analogue 8-Br-cAMP to Acvr1 siRNA-transfected stromal cells resulted in an obvious increase of Gja1 expression, whereas PKA inhibitor H89 impeded the induction of Gja1 elicited by Acvr1 overexpression, indicating that cAMP-PKA signal mediates the regulation of Acvr1 on Gja1 expression...
February 20, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28219599/aminophylline-effect-on-renal-ischemia-reperfusion-injury-in-mice
#18
K Seo, J W Choi, D-W Kim, D Han, S J Noh, H S Jung
BACKGROUND: Aminophylline increases the intracellular concentration of cAMP and exerts an anti-inflammatory effect. The aim of this study was to investigate the effect of aminophylline on renal ischemia-reperfusion (I/R) injury in mice. METHODS: Thirty C57BL/6 mice were divided into 3 groups. In the sham group (group S, n = 10), only right nephrectomy was performed. In the control group (group C, n = 10), after right nephrectomy, the mice were subjected to 30 minutes of left renal ischemia...
March 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28219521/butyrate-influences-intracellular-levels-of-adenine-and-adenine-derivatives-in-the-fungus-penicillium-restrictum
#19
Christoph Zutz, Yi Ming Chiang, Bettina Faehnrich, Markus Bacher, Roland Hellinger, Bernhard Kluger, Martin Wagner, Joseph Strauss, Kathrin Rychli
Butyrate, a small fatty acid, has an important role in the colon of ruminants and mammalians including the inhibition of inflammation and the regulation of cell proliferation. There is also growing evidence that butyrate is influencing the histone structure in mammalian cells by inhibition of histone deacetylation. Butyrate shows furthermore an antimicrobial activity against fungi, yeast and bacteria, which is linked to its toxicity at a high concentration. In fungi there are indications that butyrate induces the production of secondary metabolites potentially via inhibition of histone deacetylases...
April 2017: Microbiological Research
https://www.readbyqxmd.com/read/28219085/fibrinolysis-in-trauma-myth-reality-or-something-in-between
#20
Mark Walsh, Jacob Shreve, Scott Thomas, Ernest Moore, Hunter Moore, Daniel Hake, Tim Pohlman, Patrick Davis, Victoria Ploplis, Andres Piscoya, Julie Wegner, John Bryant, Anton Crepinsek, James Lantry, Forest Sheppard, Francis Castellino
The emphasis on fibrinolysis as an important contributor to trauma-induced coagulopathy (TIC) has led to a debate regarding the relative clinical significance of fibrinolysis in the setting of trauma. The debate has centered on two camps. The one camp defines fibrinolysis in trauma by standard coagulation tests as well as fibrin split products, D-dimers, and plasmin/antiplasmin levels. This camp favors a more liberal use of tranexamic acid and attributes more significance to hyperfibrinolysis in TIC. The other camp favors a definition of fibrinolysis based on the viscoelastic tests (VET), rotational thromboelastometry (ROTEM), and thromboelastography (TEG)...
February 20, 2017: Seminars in Thrombosis and Hemostasis
keyword
keyword
3014
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"