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https://www.readbyqxmd.com/read/28806608/differential-effects-of-childhood-trauma-subtypes-on-fatigue-and-physical-functioning-in-chronic-fatigue-syndrome
#1
Maud De Venter, Jela Illegems, Rita Van Royen, Greta Moorkens, Bernard G C Sabbe, Filip Van Den Eede
OBJECTIVE: There is wide consensus that childhood trauma plays an important role in the aetiology of chronic fatigue syndrome (CFS). The current study examines the differential effects of childhood trauma subtypes on fatigue and physical functioning in individuals suffering from CFS. METHODS: Participants were 155 well-documented adult, predominantly female CFS patients receiving treatment at the outpatient treatment centre for CFS of the Antwerp University Hospital in Belgium...
July 19, 2017: Comprehensive Psychiatry
https://www.readbyqxmd.com/read/28805677/endocytosis-of-albumin-induces-matrix-metalloproteinase-9-by-activating-the-erk-signaling-pathway-in-renal-tubule-epithelial-cells
#2
Xiaoming Chen, Alyssa Cobbs, Jasmine George, Ashmeer Chima, Fidele Tuyishime, Xueying Zhao
Matrix metalloproteinase-9 (MMP-9) is dysregulated in chronic kidney diseases including diabetic nephropathy. This study was performed to examine the expression of MMP-9 in renal tubule epithelial cells (TECs) under diabetic conditions and its regulatory mechanisms. We characterized MMP-9 protein in diabetic animals and primary cultured rat TECs exposed to exogenous albumin and high glucose. We also used specific inhibitors to determine if internalization of albumin and/or extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation were required for MMP-9 secretion...
August 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28802993/microbial-community-structure-and-activity-in-trace-element-contaminated-soils-phytomanaged-by-gentle-remediation-options-gro
#3
M Touceda-González, Á Prieto-Fernández, G Renella, L Giagnoni, A Sessitsch, G Brader, J Kumpiene, I Dimitriou, J Eriksson, W Friesl-Hanl, R Galazka, J Janssen, M Mench, I Müller, S Neu, M Puschenreiter, G Siebielec, J Vangronsveld, P S Kidd
Gentle remediation options (GRO) are based on the combined use of plants, associated microorganisms and soil amendments, which can potentially restore soil functions and quality. We studied the effects of three GRO (aided-phytostabilisation, in situ stabilisation and phytoexclusion, and aided-phytoextraction) on the soil microbial biomass and respiration, the activities of hydrolase enzymes involved in the biogeochemical cycles of C, N, P, and S, and bacterial community structure of trace element contaminated soils (TECS) from six field trials across Europe...
August 10, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28801669/balance-between-estrogens-and-proinflammatory-cytokines-regulates-chemokine-production-involved-in-thymic-germinal-center-formation
#4
Nadine Dragin, Patrice Nancy, José Villegas, Régine Roussin, Rozen Le Panse, Sonia Berrih-Aknin
The early-onset form of Myasthenia Gravis (MG) is prevalent in women and associates with ectopic germinal centers (GCs) development and inflammation in the thymus. we aimed to investigate the contribution of estrogens in the molecular processes involved in thymic GCs formation. We examined expression of genes involved in anti-acetylcholine receptor (AChR) response in MG, MHC class II and α-AChR subunit as well as chemokines involved in GC development (CXCL13, CCL21and CXCL12). In resting conditions, estrogens have strong regulatory effects on thymic epithelial cells (TECs), inducing a decreased protein expression of the above molecules...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28800844/first-comparative-analysis-concerning-the-plasma-platelet-contamination-during-mnc-collection
#5
Hella Pfeiffer, Susanne Achenbach, Julian Strobel, Robert Zimmermann, Reinhold Eckstein, Erwin F Strasser
BACKGROUND AND OBJECTIVES: Monocytes can be cultured into dendritic cells with addition of autologous plasma, which is highly prone to platelet contamination due to the apheresis process. Since platelets affect the maturation process of monocytes into dendritic cells and might even lead to a diminished harvest of dendritic cells, it is very important to reduce the platelet contamination. A new collection device (Spectra Optia) was analyzed, compared to two established devices (COM.TEC, Cobe Spectra) and evaluated regarding the potential generation of source plasma...
July 13, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28777905/decompression-sickness-among-diving-fishermen-in-mexico-observational-retrospective-analysis-of-dcs-in-three-sea-cucumber-fishing-seasons
#6
Oswaldo Huchim-Lara, Walter Chin, Silvia Salas, Normando Rivera-Canul, Salvador Cordero-Romero, Juan Tec, Ellie Joo, Nina Mendez-Dominguez
The probabilities of decompression sickness (DCS) among diving fishermen are higher than in any other group of divers. Diving behavior of artisanal fishermen has been directed mainly to target high-value species. The aim of this study was to learn about the occurrence of DCS derived from sea cucumber harvesting in the Yucatán Peninsula, Mexico. We conducted a retrospective chart review of diving fishermen treated at a multiplace hyperbaric chamber in Tizimín, Mexico. In total, 233 recompression therapies were rendered to 166 diving fishermen from 2014 to 2016...
March 2017: Undersea & Hyperbaric Medicine: Journal of the Undersea and Hyperbaric Medical Society, Inc
https://www.readbyqxmd.com/read/28767893/numerical-simulations-to-assess-art-and-mart-performance-for-ionospheric-tomography-of-chapman-profiles
#7
Fabricio S Prol, Paulo O Camargo, Marcio T A H Muella
The incomplete geometrical coverage of the Global Navigation Satellite System (GNSS) makes the ionospheric tomographic system an ill-conditioned problem for ionospheric imaging. In order to detect the principal limitations of the ill-conditioned tomographic solutions, numerical simulations of the ionosphere are under constant investigation. In this paper, we show an investigation of the accuracy of Algebraic Reconstruction Technique (ART) and Multiplicative ART (MART) for performing tomographic reconstruction of Chapman profiles using a simulated optimum scenario of GNSS signals tracked by ground-based receivers...
July 31, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28763383/addressing-data-inequities-in-american-indian-communities-through-an-environmental-public-health-tracking-pilot-project
#8
Samantha Lucas-Pipkorn, Ashley Tuomi
National and state surveillance systems identify American Indians/Alaska Natives inconsistently and often inaccurately within their data sets. Consequently, communities may not be represented with data at the level of geography needed. Collecting and presenting environmental and health-related data to the public are done by state Tracking Networks. Great Lakes Inter-Tribal Epidemiology Center (GLITEC) conducted a groundbreaking 2014 Tribal Environmental Health Tracking pilot project that engaged tribes, an urban Indian community, 3 state health departments, a Tribal Epidemiology Center (TEC), and a federal agency...
September 2017: Journal of Public Health Management and Practice: JPHMP
https://www.readbyqxmd.com/read/28760969/factor-dependent-archaeal-transcription-termination
#9
Julie E Walker, Olivia Luyties, Thomas J Santangelo
RNA polymerase activity is regulated by nascent RNA sequences, DNA template sequences, and conserved transcription factors. Transcription factors promoting initiation and elongation have been characterized in each domain, but transcription termination factors have been identified only in bacteria and eukarya. Here we describe euryarchaeal termination activity (Eta), the first archaeal termination factor capable of disrupting the transcription elongation complex (TEC), detail the rate of and requirements for Eta-mediated transcription termination, and describe a role for Eta in transcription termination in vivo...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28756542/bioethanol-production-from-soybean-residue-via-separate-hydrolysis-and-fermentation
#10
Trung Hau Nguyen, Chae Hun Ra, In Yung Sunwoo, Pailin Sukwong, Gwi-Taek Jeong, Sung-Koo Kim
Bioethanol was produced using polysaccharide from soybean residue as biomass by separate hydrolysis and fermentation (SHF). This study focused on pretreatment, enzyme saccharification, and fermentation. Pretreatment to obtain monosaccharide was carried out with 20% (w/v) soybean residue slurry and 270 mmol/L H2SO4 at 121 °C for 60 min. More monosaccharide was obtained from enzymatic hydrolysis with a 16 U/mL mixture of commercial enzymes C-Tec 2 and Viscozyme L at 45 °C for 48 h. Ethanol fermentation with 20% (w/v) soybean residue hydrolysate was performed using wild-type and Saccharomyces cerevisiae KCCM 1129 adapted to high concentrations of galactose, using a flask and 5-L fermenter...
July 29, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28744941/removal-of-cell-activating-substances-using-dialyzers-with-various-permeability-profiles
#11
Agnieszka Latosinska, Michael Hulko, Rose Speidel, Harald Mischak, Markus Storr, Bernd Krause
Despite multiple efforts to target an improvement in clinical outcomes of patients with end-stage renal disease, several challenges must still be addressed. Dialysis patients are at a high risk for complications, as reflected by increasing mortality rates. The objective of this study is to assess the impact of the application of dialyzers with varying permeability profiles on the removal of cell-activating substances from the blood of hemodialysis (HD) patients. Dialysate samples were collected using Revaclear 400 (RC) and MCO-Ci400 (MCO-CI)...
July 26, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28739827/transcriptional-profiling-of-the-chicken-tracheal-response-to-virulent-mycoplasma-gallisepticum-strain-rlow
#12
J Beaudet, E R Tulman, K Pflaum, X Liao, G F Kutish, S M Szczepanek, L K Silbart, S J Geary
Mycoplasma gallisepticum, the primary etiologic agent of Chronic Respiratory Disease (CRD) in poultry, leads to prolonged recruitment and activation of inflammatory cells in the respiratory mucosa. This is consistent with the current model of immune dysregulation that ostensibly allows the organism to evade clearance mechanisms and establish chronic infection. To date, studies using qRT-PCR and microarrays have shown a significant transient up-regulation of cytokines and chemokines from tracheal epithelial cells (TECs) in vitro and tracheal tissue ex vivo in response to virulent strain Rlow, that contributes to the infiltration of inflammatory cells into the tracheal mucosa...
July 24, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28739013/influence-of-access-cavity-design-on-root-canal-detection-instrumentation-efficacy-and-fracture-resistance-assessed-in-maxillary-molars
#13
Gabriela Rover, Felipe Gonçalves Belladonna, Eduardo Antunes Bortoluzzi, Gustavo De-Deus, Emmanuel João Nogueira Leal Silva, Cleonice Silveira Teixeira
INTRODUCTION: The aim of this study was to assess the influence of contracted endodontic cavities (CECs) on root canal detection, instrumentation efficacy, and fracture resistance assessed in maxillary molars. Traditional endodontic cavities (TECs) were used as a reference for comparison. METHODS: Thirty extracted intact maxillary first molars were scanned with micro-computed tomographic imaging at a resolution of 21 μm, assigned to the CEC or TEC group (n = 15/group), and accessed accordingly...
July 21, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28730234/associated-lesions-of-peri-implant-mucosa-in-immediate-versus-delayed-loading-of-dental-implants
#14
Alexandru Andrei Iliescu, Sabina Andrada Zurac, Vasile Nicolae, Mihaela Georgiana Iliescu, Paula Perlea
Currently, immediate loading of dental implants is very attractive as a standard protocol for prosthetic restorations in edentulous patients. The aim of this study is to find out the intimate peri-implant mucosa response depending on timing of implant loading, immediate or delayed. Fifty-one screw implants Alpha Bio (Alpha-Bio Tec, Israel) were inserted in 42 partially edentulous patients according to standardized surgical techniques. At six months of loading, samples of peri-implant mucosa were harvested from 27 immediate loaded, respectively 24 delayed loaded implants, and subjected to microscopic examination...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28726483/understanding-the-context-of-novel-interventions-for-self-injurious-thoughts-and-behaviors-a-reply-to-nielsen-et-al
#15
Joseph C Franklin, Kathryn R Fox, Jessica D Ribeiro, Matthew K Nock
In this response to the commentary by Nielsen, Kirtley, & Townsend (in press), the authors note several points of agreement related to further testing of therapeutic evaluative conditioning (TEC) for self-injurious thoughts and behaviors (SITBs), navigating logistical and ethical issues surrounding mobile interventions, and integrating such interventions into traditional practice. They also note a common mission held by Nielsen et al., themselves, and the SITB research community more broadly: the large-scale reduction of SITBs...
August 2017: Journal of Consulting and Clinical Psychology
https://www.readbyqxmd.com/read/28726482/-great-powers-and-great-responsibilities-a-brief-comment-on-a-brief-mobile-app-reduces-nonsuicidal-and-suicidal-self-injury-evidence-from-three-randomized-controlled-trials-franklin-et-al-2016
#16
Emma Nielsen, Olivia J Kirtley, Ellen Townsend
Online and mobile mental health applications (apps) herald exciting new opportunities for the treatment and prevention of self-injurious thoughts and behaviors (SITBs). With such rapid technological advances, it is paramount that health care innovation not be achieved to the detriment of intervention quality. Franklin et al.'s (2016) therapeutic evaluative conditioning (TEC) app is a novel and timely addition to the mobile health landscape; uncommonly for such apps, it is evidence based. There are, however, several crucial challenges to be surmounted for TEC to be successful; arguably, interventions ought to build lasting skills that can be subsequently and consciously recruited to manage distress beyond the intervention period...
August 2017: Journal of Consulting and Clinical Psychology
https://www.readbyqxmd.com/read/28724101/assessment-of-the-effects-of-total-emission-control-policies-on-surface-water-quality-in-china-2004-to-2014
#17
Xia Liu, Jianfeng Feng, Yinhuan Qiao, Yuqiu Wang, Lin Zhu
Water quality deterioration is a problem throughout the globe, especially in China. The Chinese government established national total emission control (TEC) policies related to the nation's surface water quality over the past 10 yr to restore, protect, and promote healthy water quality. The objective of this study is to evaluate the effects of China's TEC policies by analyzing three water quality constituents (ammonium nitrogen [NH-N], dissolved oxygen [DO], and the permanganate index [COD]) at 73 monitoring stations, which account for most of the surface waters of eastern China...
May 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28723666/increased-cflip-expression-in-thymic-epithelial-tumors-blocks-autophagy-via-nf-%C3%AE%C2%BAb-signalling
#18
Djeda Belharazem, Albert Grass, Cornelia Paul, Mario Vitacolonna, Berthold Schalke, Ralf J Rieker, Daniel Körner, Philipp Jungebluth, Katja Simon-Keller, Peter Hohenberger, Eric M Roessner, Karsten Wiebe, Thomas Gräter, Thomas Kyriss, German Ott, Peter Geserick, Martin Leverkus, Philipp Ströbel, Alexander Marx
The anti-apoptotic cellular FLICE-like inhibitory protein cFLIP plays a pivotal role in normal tissues homoeostasis and the development of many tumors, but its role in normal thymus (NT), thymomas and thymic carcinomas (TC) is largely unknown.Expression, regulation and function of cFLIP were analyzed in biopsies of NT, thymomas, thymic squamous cell carcinomas (TSCC), thymic epithelial cells (TECs) derived thereof and in the TC line 1889c by qRT-PCR, western blot, shRNA techniques, and functional assays addressing survival, senescence and autophagy...
February 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28722272/development-of-a-thiol-ene-based-screening-platform-for-enzyme-immobilization-demonstrated-using-horseradish-peroxidase
#19
Christian Hoffmann, Manuel Pinelo, John M Woodley, Anders E Daugaard
Efficient immobilization of enzymes on support surfaces requires an exact match between the surface chemistry and the specific enzyme. A successful match would normally be identified through time consuming screening of conventional resins in multiple experiments testing individual immobilization strategies. In this study we present a versatile strategy that largely expands the number of possible surface functionalities for enzyme immobilization in a single, generic platform. The combination of many individual surface chemistries and thus immobilization methods in one modular system permits faster and more efficient screening, which we believe will result in a higher chance of discovery of optimal surface/enzyme interactions...
July 19, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28719051/the-binding-mechanism-between-azoles-and-fgcyp51b-sterol-14%C3%AE-demethylase-of-fusarium-graminearum
#20
Hengwei Qian, Meilin Duan, Xiaomei Sun, Mengyu Chi, Ying Zhao, Wenxing Liang, Juan Du, Jinguang Huang, Baodu Li
BACKGROUND: Fusarium graminearum is the main pathogen of Fusarium Head Blight (FHB), a worldwide plant disease and one of the major wheat diseases in China. The control of the FHB is mainly dependent on the application of DMIs fungicides. Fungal sterol 14α-demethylase enzymes (CYP51) are the main target for DMIs fungicides. In order to investigate the binding mechanism between azoles and CYP51B in F.graminearum, the molecular modeling study and biological evaluation were performed. RESULTS: Firstly, the homology model based on the crystal structure of Aspergillus fumigatus was built...
July 18, 2017: Pest Management Science
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