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https://www.readbyqxmd.com/read/29353307/characterization-of-an-%C3%AE-agarase-from-thalassomonas-sp-ld5-and-its-hydrolysate
#1
Weibin Zhang, Jingnan Xu, Dan Liu, Huan Liu, Xinzhi Lu, Wengong Yu
It has been a long time since the first α-agarase was discovered. However, only two α-agarases have been cloned and partially characterized so far and the study of α-agarases has lagged far behind that of β-agarases. Here, we report an α-agarase, AgaD, cloned from marine bacterium Thalassomonas sp. LD5. Its cDNA consists of 4401 bp, encoding a protein of 1466 amino acids. Based on amino acid similarity, AgaD is classified into glycoside hydrolase (GH) family GH96. The recombinant enzyme gave a molecular weight of about 180 kDa on SDS-PAGE and 360 kDa on Native-PAGE indicating it acted as a dimer...
January 20, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29353270/screening-for-brain-involvement-in-infants-with-multifocal-cutaneous-infantile-hemangiomas
#2
Rivka Friedland, Dan Ben Amitai, Alex Zvulunov
BACKGROUND: Multifocal (≥5) infantile hemangiomas (IHs) are known as a risk factor for extracutaneous involvement. Liver is the most commonly involved organ, but involvement of other systems has also been reported. This study aims to describe the characteristic findings in a group of infants with multiple cutaneous hemangiomas, with emphasis on intracranial involvement. METHODS: A retrospective case series study was carried out in a pediatric dermatology unit of a tertiary pediatric medical center...
January 20, 2018: Dermatology: International Journal for Clinical and Investigative Dermatology
https://www.readbyqxmd.com/read/29353204/mir-152-inhibits-proliferation-of-human-endometrial-cancer-cells-via-inducing-g2-m-phase-arrest-by-suppressing-cdc25b-expression
#3
Dan Xie, Yijuan Liang, Yuanyuan Su, Yan An, Pengpeng Qu
microRNA-152 (miR-152) is a tumor suppressor that is down-regulated in many cancers including endometrial cancer (EC). However, the underlying mechanism of action of miR-152 in EC is unclear. The aim of the present study was to evaluate the role of miR-152 on proliferation of human endometrial cancer cells. Herein, we found that miR-152 overexpression and CDC25B knockdown inhibited proliferative ability and induced G2/M phase arrest in KLE and HEC-1B cells. CDC25B was a target of miR-152. In addition, CDC25B overexpression rescued miR-152-induced proliferation inhibition and G2/M phase arrest in human endometrial cancer cells...
January 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29353068/mitochondrial-atp-sensitive-potassium-channel-regulates-mitochondrial-dynamics-to-participate-in-neurodegeneration-of-parkinson-s-disease
#4
Kaige Peng, Jun Hu, Jingsong Xiao, Guorong Dan, Likui Yang, Feng Ye, Zhongmin Zou, Jia Cao, Yan Sai
Parkinson's disease (PD) is the second most common age-related neurodegenerative disease. Mitochondrial dysfunction has been the focus of the pathogenesis of PD. The mitochondrial ATP-sensitive potassium channel (mitoKATP) plays a significant role in mitochondrial physiology and has been extensively shown to protect against ischemic and brain reperfusion injury. However, there have long been controversies regarding its role in Parkinson's disease. We investigated the role of mitoKATP channels in rotenone-induced PD model in vivo and vitro and the interactions of mitoKATP channels, mitochondrial dynamics and PD...
January 15, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29352975/pinocembrin-from-penthorum-chinense-pursh-suppresses-hepatic-stellate-cells-activation-through-a-unified-sirt3-tgf-%C3%AE-smad-signaling-pathway
#5
Fayang Zhou, Anqi Wang, Dan Li, Yitao Wang, Ligen Lin
The inactivation of hepatic stellate cells (HSCs) has been verified to be an effective therapeutic strategy for treatment of liver fibrosis. Penthorum chinense Pursh has been widely used to protect liver in China; while, the role of P. chinense Pursh in treatment of liver fibrosis is still unexplored. In the current study, the aqueous extract of P. chinense Pursh (PCE) was found to suppress the expressions of fibrotic markers, including collagen I and α-smooth muscle actin (α-SMA), in human HSCs (LX-2); and its major active constituent, pinocembrin (PIN), was discovered to inhibit the expressions of fibrotic markers in LX-2 cells and rat HSCs (HSC-T6)...
January 15, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29352742/cambogin-suppresses-dextran-sulphate-sodium-induced-colitis-by-enhancing-treg-cell-stability-and-function
#6
Yue Lu, Na-Mi Kim, Yi-Wen Jiang, Hong Zhang, Dan Zheng, Fu-Xiang Zhu, Rui Liang, Bin Li, Hong-Xi Xu
BACKGROUND AND PURPOSE: Inflammatory bowel disease (IBD) is a chronic and relapsing inflammatory disorder of gastrointestinal tract, and the dysfunction of immune regulation plays a critical role in IBD. Reflecting the limited use of current drugs and therapies for IBD treatment, it is necessary to explore novel drugs or therapies for this disease. We investigated the effect of cambogin in a mouse model of dextran sulphate sodium (DSS)-induced colitis and whether cambogin attenuates inflammation via Treg-cell-mediated immune modulation...
January 20, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29352480/temporal-heterogeneity-increases-with-spatial-heterogeneity-in-ecological-communities
#7
Scott L Collins, Meghan L Avolio, Corinna Gries, Lauren M Hallett, Sally E Koerner, Kimberly J La Pierre, Andrew L Rypel, Eric R Sokol, Samuel B Fey, Dan F B Flynn, Sydney K Jones, Laura M Ladwig, Julie Ripplinger, Matt B Jones
Heterogeneity is increasingly recognized as a foundational characteristic of ecological systems. Under global change, understanding temporal community heterogeneity is necessary for predicting the stability of ecosystem functions and services. Indeed, spatial heterogeneity is commonly used in alternative stable state theory as a predictor of temporal heterogeneity and therefore an early indicator of regime shifts. To evaluate whether spatial heterogeneity in species composition is predictive of temporal heterogeneity in ecological communities, we analyzed 68 community datasets spanning freshwater and terrestrial systems where measures of species abundance were replicated over space and time...
January 20, 2018: Ecology
https://www.readbyqxmd.com/read/29352428/hnf4%C3%AE-is-involved-in-the-regulation-of-vertebrate-lc-pufa-biosynthesis-insights-into-the-regulatory-role-of-hnf4%C3%AE-on-expression-of-liver-fatty-acyl-desaturases-in-the-marine-teleost-siganus-canaliculatus
#8
Shuqi Wang, Junliang Chen, Danli Jiang, Qinghao Zhang, Cuihong You, Douglas R Tocher, Óscar Monroig, Yewei Dong, Yuanyou Li
Long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis is an important metabolic pathway in vertebrates, especially fish, considering they are the major source of n-3 LC-PUFA in the human diet. However, most fish have only limited capability for biosynthesis of LC-PUFA. The rabbitfish (Siganus canaliculatus) is able to synthesize LC-PUFA as it has all the key enzyme activities required including Δ6Δ5 Fads2, Δ4 Fads2, Elovl5, and Elovl4. We previously reported a direct interaction between the transcription factor Hnf4α and the promoter regions of Δ4 and Δ6Δ5 Fads2, which suggested that Hnf4α was involved in the transcriptional regulation of fads2 in rabbitfish...
January 19, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29352255/offline-stimulation-of-human-parietal-cortex-differently-affects-resting-eeg-microstates
#9
Pierpaolo Croce, Filippo Zappasodi, Paolo Capotosto
The interference effects of transcranial magnetic stimulation (TMS) on several electroencephalographic (EEG) measures in both temporal and frequency domains have been reported. We tested the hypothesis whether the offline external inhibitory interference, although focal, could result in a global reorganization of the functional brain state, as assessed by EEG microstates. In 16 healthy subjects, we inhibited five parietal areas and used a pseudo stimulation (Sham) at rest. The EEG microstates were extracted before and after each stimulation...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29352142/fbxl10-contributes-to-the-development-of-diffuse-large-b-cell-lymphoma-by-epigenetically-enhancing-erk1-2-signaling-pathway
#10
Xiujuan Zhao, Xing Wang, Qian Li, Wanbiao Chen, Na Zhang, Yu Kong, Junqiang Lv, Lei Cao, Dan Lin, Xi Wang, Guogang Xu, Xudong Wu
Epigenetic modifiers have emerged as critical factors governing the biology of different cancers. Herein we show that FBXL10 (also called KDM2B or JHDM1B), an important member of Polycomb repressive complexes, is overexpressed in human diffuse large B-cell lymphoma (DLBCL) tissues and the derived cell lines. Knocking down FBXL10 by specific short hairpin RNAs in DLBCL cells inhibits cell proliferation and induces apoptosis in vitro. Moreover, FBXL10 depletion in DLBCL cells abrogates tumor growth in mouse xenograft models...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29351253/the-role-of-sugar-transporter-genes-during-early-infection-by-root-knot-nematodes
#11
Dan Zhao, Yang You, Haiyan Fan, Xiaofeng Zhu, Yuanyuan Wang, Yuxi Duan, Yuanhu Xuan, Lijie Chen
Although pathogens such as nematodes are known to hijack nutrients from host plants, the mechanisms whereby nematodes obtain sugars from plants remain largely unknown. To determine the effects of nematode infection on host plant sugar allocation, soluble sugar (fructose, glucose, sucrose) content was investigated using high-performance liquid chromatography with refractive index detection and was found to increase significantly in tomato (Solanum lycopersicum, Sl) leaves and roots during early infection by root-knot nematodes (RKNs)...
January 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29351088/fraction-variant-beam-orientation-optimization-for-non-coplanar-imrt
#12
Daniel O'Connor, Victoria Yu, Dan Nguyen, Dan Ruan, Ke Sheng
Conventional beam orientation optimization (BOO) algorithms for IMRT assume that the same set of beam angles is used for all treatment fractions. In this paper we present a BOO formulation based on group sparsity that simultaneously optimizes non-coplanar beam angles for all fractions, yielding a fraction-variant (FV) treatment plan. Beam angles are selected by solving a multi-fraction fluence map optimization problem involving 500-700 candidate beams per fraction, with an additional group sparsity term that encourages most candidate beams to be inactive...
January 19, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29350956/negative-mass-instability-of-the-spin-and-motion-of-an-atomic-gas-driven-by-optical-cavity-backaction
#13
Jonathan Kohler, Justin A Gerber, Emma Dowd, Dan M Stamper-Kurn
We realize a spin-orbit interaction between the collective spin precession and center-of-mass motion of a trapped ultracold atomic gas, mediated by spin- and position-dependent dispersive coupling to a driven optical cavity. The collective spin, precessing near its highest-energy state in an applied magnetic field, can be approximated as a negative-mass harmonic oscillator. When the Larmor precession and mechanical motion are nearly resonant, cavity mediated coupling leads to a negative-mass instability, driving exponential growth of a correlated mode of the hybrid system...
January 5, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/29350525/a-microporous-luminescent-metal-organic-framework-for-a-sensitive-and-selective-fluorescence-sensing-of-toxic-mycotoxin-in-mouldy-sugarcane
#14
Dan Tian, Xiao-Jing Liu, Rui Feng, Jia-Li Xu, Jian Xu, Rong-Ying Chen, Ling Huang, Xian-He Bu
Food contamination by toxic mycotoxins not only causes a considerable loss in economy, but importantly poses a huge threat to human health through accidental ingestion. Hence, it is an ongoing and imperative need to develop a convenient, cost-effective method for the detection of the mycotoxin-infected agricultural commodities. To this end, we herein fabricated a novel metal-organic framework (MOF) derived composite material that displays a strong solid-state emission in the visible region, by attaching a frequently used fluorescent label, fluorescein isothiocyanate (FITC), via guest adsorption...
January 19, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29350191/a-prospective-gating-method-to-acquire-a-diverse-set-of-free-breathing-ct-images-for-model-based-4dct
#15
Dylan O'Connell, Dan Ruan, David H Thomas, Tai H Dou, John H Lewis, Anand P Santhanam, Percy P Lee, Daniel A Low
Breathing motion modeling requires observation of tissues at sufficiently distinct respiratory states for proper 4D characterization. This work proposes a method to improve sampling of the breathing cycle with limited imaging dose. We designed and tested a prospective free-breathing acquisition protocol with a simulation using datasets from 5 patients imaged with a model-based 4DCT technique. Each dataset contained 25 free-breathing fast helical CT scans with simultaneous breathing surrogate measurements...
January 19, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29349978/-correlation-of-medial-compartmental-joint-line-elevation-with-femorotibial-angle-correction-and-clinical-function-after-unicompartmental-arthroplasty
#16
Zhan-Feng Zhang, Dan Wang, Ji-Kang Min
OBJECTIVE: To study the correlation of postoperative femorotibial angle with medial compartmental joint line elevation after unicompartmental arthroplasty(UKA), as well as the correlation of joint line elevation with the clinical function by measuring radiological joint line. METHODS: A retrospective study of 56 patients from July 2012 to August 2015 was performed. The mean body mass index (BMI) was 23.5 (ranged, 18.3 to 30.1). The standing anteroposterior radiographs of these patients were assessed both pre-and post-operatively, and the knee function was evaluated according to HSS grading...
April 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29349799/simultaneous-lc-ms-ms-bioanalysis-of-etoposide-and-paclitaxel-in-mouse-tissues-and-plasma-after-oral-administration-of-self-microemulsifying-drug-delivery-systems
#17
Dan Li, Gang Zhao, Weipeng Ai, Gao Li, Luqin Si, Jiangeng Huang, Yunzhou Chen, Sanlan Wu
In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determine the anticancer drugs etoposide and paclitaxel in mouse plasma and tissues including liver, kidney, lung, heart, spleen and brain. The analytes were extracted from the matrices of interest by liquid-liquid extraction using DCM: MTBE (1:1, v/v). Chromatographic separation was achieved on an Ultimate XB-C18 column (100 mm × 2.1 mm, 3 μm) at 40 °C and the total run time was 4 min under a gradient elution...
January 19, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29349790/identification-of-the-absorbed-components-and-metabolites-of-modified-huo-luo-xiao-ling-dan-in-rat-plasma-by-uhplc-q-tof-ms-ms
#18
Nannan Wang, Xiaoning Zhao, Yiran Li, Congcong Cheng, Jiaxin Huai, Kaishun Bi, Ronghua Dai
To reveal the material basis of Huo Luo Xiao Ling Dan, a sensitive and selective ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method was developed to identify the absorbed components and metabolites in rat plasma after oral administration of HLXLD. The plasma samples were pretreated by liquid-liquid extraction and separated on a Shim-pack XR-ODS C18 column (75 mm × 3.0 mm, 2.2 μm) using a gradient elution program. With the optimized conditions and single sample injection of each positive or negative ion mode, a total of 109 compounds, including 78 prototype compounds and 31 metabolites were identified or tentatively characterized...
January 19, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29349494/light-scattering-on-pha-granules-protects-bacterial-cells-against-the-harmful-effects-of-uv-radiation
#19
Eva Slaninova, Petr Sedlacek, Filip Mravec, Lucie Mullerova, Ota Samek, Martin Koller, Ondrej Hesko, Dan Kucera, Ivana Marova, Stanislav Obruca
Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in microbial cells enhances their stress resistance and fitness when exposed to various stress factors. In this work, we studied the protective mechanism of PHA granules against UV irradiation employing Cupriavidus necator as a model bacterial strain. The PHA-accumulating wild type strain showed substantially higher UV radiation resistance than the PHA non-accumulating mutant...
January 19, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29348722/regulatory-effects-of-saponins-from-panax-japonicus-on-colonic-epithelial-tight-junctions-in-aging-rats
#20
Yaoyan Dun, Min Liu, Jing Chen, Danli Peng, Haixia Zhao, Zhiyong Zhou, Ting Wang, Chaoqi Liu, Yuhui Guo, Changcheng Zhang, Ding Yuan
Background: Saponins from Panax japonicus (SPJ) are the most abundant and main active components of P. japonicus, which replaces ginseng roots in treatment for many kinds of diseases in the minority ethnic group in China. Our previous studies have demonstrated that SPJ has the effects of anti-inflammation through the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways. The present study was designed to investigate whether SPJ can modulate intestinal tight junction barrier in aging rats and further to explore the potential mechanism...
January 2018: Journal of Ginseng Research
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