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Hyaluronan

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https://www.readbyqxmd.com/read/29040808/independently-tuning-the-biochemical-and-mechanical-properties-of-3d-hyaluronan-based-hydrogels-with-oxime-and-diels-alder-chemistry-to-culture-breast-cancer-spheroids
#1
Alexander E G Baker, Roger Y Tam, Molly S Shoichet
In order to mimic native breast cancer cell growth in vitro as spheroids, a well-defined matrix that mimics the tumor microenvironment is required. Finding a biomimetic material for 3D cell culture, other than Matrigel, has challenged the field. Since hyaluronan is naturally abundant in the tumor microenvironment and can be chemically modified, we synthesized a hyalu-ronan hydrogel with independently tunable mechanical and chemical properties for 3D culture of breast cancer cells. By modifying HA with distinct bioorthogonal functional groups, its mechanical properties are controlled by chemical crosslinking via oxime liga-tion while its biochemical properties are controlled by grafting bioactive peptides via Diels-Alder chemistry...
October 17, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29035502/binding-mechanism-of-model-charged-dye-carboxyfluorescein-to-hyaluronan-polylysine-multilayers
#2
Vladimir Z Prokopovic, Anna S Vikulina, David Sustr, Elena M Shchukina, Dmitry G Shchukin, Dmitry V Volodkin
Biopolymer-based multilayers become more and more attractive due to the vast span of biological application they can be used for, e.g. implant coatings, cell culture supports, scaffolds. Multilayers have demonstrated superior capability to store enormous amount of small charged molecules such as drugs and release them in a controlled manner, however, the binding mechanism for drug loading into the multilayers is still poorly understood. Here we focus on this mechanism using model hyaluronan/polylysine (HA/PLL) multilayers and a model charged dye carboxyfluorescein (CF)...
October 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29025676/a-simple-and-efficient-feeder-free-culture-system-to-up-scale-ipscs-on-polymeric-material-surface-for-use-in-3d-bioprinting
#3
Chui-Wei Wong, You-Tzung Chen, Chung-Liang Chien, Tien-Yu Yu, Syang-Peng Rwei, Shan-Hui Hsu
The 3D bioprinting and cell/tissue printing techniques open new possibilities for future applications. To facilitate the 3D bioprinting process, a large amount of living cells are required. Induced pluripotent stem cells (iPSCs) represent a promising cell source for bioprinting. However, the maintenance and expansion of undifferentiated iPSCs are expensive and time consuming. Therefore, in this study a culture method to obtain a sufficient amount of healthy and undifferentiated iPSCs in a short-term period was established...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29025668/sustained-raloxifene-release-from-hyaluronan-alendronate-functionalized-titanium-nanotube-arrays-capable-of-enhancing-osseointegration-in-osteoporotic-rabbits
#4
Caiyun Mu, Yan Hu, Ling Huang, Xinkun Shen, Menghuan Li, Liqi Li, Hao Gu, Yonglin Yu, Zhining Xia, Kaiyong Cai
To enhance the localized bone remodeling at titanium-based implants under osteoporotic conditions, TiO2 nanotube arrays (TNT) were used as nanoreserviors for raloxifene (Ral) and then covered with the hybrid multilayered coating of chitosan and alendronate grafted hyaluronic acid (HA-Aln) via a spin-assisted layer-by-layer technique. The fabrication of this system (TNT/Ral/LBL-Aln) was characterized by field emission scanning electron microscopy (SEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS), respectively...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29024602/knockdown-of-cemip-suppresses-proliferation-and-induces-apoptosis-in-colorectal-cancer-cells-downregulation-of-grp78-and-attenuation-of-unfolded-protein-response
#5
Guodong Liang, Xuedong Fang, Yubo Yang, Yan Song
Cell migration inducing hyaluronan binding protein (CEMIP) has been suggested as a contributor in the carcinogenesis of colorectal cancer (CRC). Cancer cells can adapt to endoplasmic reticulum (ER) stress by initiating unfolded protein response (UPR). This study aimed at investigating whether CEMIP affected the UPR of CRC cells, with a focus on 78 kDa glucose-regulated protein (GRP78, a major ER chaperone). We found that knockdown of CEMIP inhibited cell proliferation and induced a G1 arrest in SW480 CRC cells...
October 12, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29024378/the-cyclocalyx-study-ovulatory-cycle-affects-circulating-compartments-of-the-endothelial-glycocalyx-in-blood
#6
Nikolai Hulde, Nina Rogenhofer, Florian Brettner, Nicole C Eckert, Isabella Götzfried, Thu Nguyen, Judith-I Pagel, Tobias Kammerer, Klaus F Hofmann-Kiefer, Gustav Schelling, Andreas Dendorfer, Markus Rehm, Christian J Thaler
PROBLEM: The endothelial glycocalyx (EGX) plays an important role in vascular integrity. Recently, increased levels of EGX components were detected in the circulating blood of healthy pregnant women and were related to the increased tendency to edema formation during gestation. However, the EGX has not yet been systematically studied in non-pregnant women during ovulatory cycles. METHOD OF STUDY: Serum levels of EGX components syndecan-1, heparan sulfate, and hyaluronan in healthy women (n = 16) at 3 phases of the ovulatory cycle (early follicular phase, at ovulation, and mid-luteal phase) were compared with a control group of healthy men (n = 10)...
October 11, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29017608/autologous-mesenchymal-stem-cells-or-meniscal-cells-what-is-the-best-cell-source-for-regenerative-meniscus-treatment-in-an-early-osteoarthritis-situation
#7
Johannes Zellner, Girish Pattappa, Matthias Koch, Siegmund Lang, Johannes Weber, Christian G Pfeifer, Michael B Mueller, Richard Kujat, Michael Nerlich, Peter Angele
BACKGROUND: Treatment of meniscus tears within the avascular region represents a significant challenge, particularly in a situation of early osteoarthritis. Cell-based tissue engineering approaches have shown promising results. However, studies have not found a consensus on the appropriate autologous cell source in a clinical situation, specifically in a challenging degenerative environment. The present study sought to evaluate the appropriate cell source for autologous meniscal repair in a demanding setting of early osteoarthritis...
October 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28993846/-hyaluronan-as-a%C3%A2-key-for-accelerated-wound-healing-in-human-3d-full-thickness-skin-models
#8
L Rüther, L Bolke, G R Schlippe, W A Voss
BACKGROUND: Wound healing can be divided into three phases: (1) exsudation phase, (2) granulation phase, (3) regeneration phase. In particular, the epithelization phase is of great importance in order to quickly reconstitute the natural skin barrier. The aim of the present study was to determine the reepithelization kinetics of untreated and 0.5% sodium hyaluronate (NHA) treated human 3D full thickness skin models. MATERIALS AND METHODS: The test protocol consisted of topically applying 10 µl of the test substance 0...
October 9, 2017: Der Hautarzt; Zeitschrift Für Dermatologie, Venerologie, und Verwandte Gebiete
https://www.readbyqxmd.com/read/28993511/cell-cycle-dependent-tumor-engraftment-and-migration-are-enabled-by-aurora-a
#9
Tony L H Chu, Marisa Connell, Lixin Zhou, Zhengcheng He, Jennifer R Won, Seyed M Rahavi, Helen Chen, Pooja Mohan, Oksana Nemirovsky, Abbas Fotovati, Miguel Angel Pujana, Gregor Sd Reid, Torsten O Nielsen, Nelly Pante, Christopher A Maxwell
Cell cycle progression and the acquisition of a migratory phenotype are hallmarks of human carcinoma cells that are perceived as independent processes but may be interconnected by molecular pathways that control microtubule nucleation at centrosomes. Here, cell cycle progression dramatically impacts the engraftment kinetics of 4T1-luciferase2 breast cancer cells in immunocompetent BALB/c or immunocompromised NOD-SCID gamma (NSG) mice. Multi-parameter imaging of wound closure assays was used to track cell cycle progression, cell migration, and associated phenotypes in epithelial cells or carcinoma cells expressing a fluorescence ubiquitin cell cycle indicator...
October 9, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28990250/galectin-3-is-persistently-increased-in-early-rheumatoid-arthritis-ra-and-associates-with-anti-ccp-seropositivity-and-mri-bony-lesions-while-early-fibrosis-markers-correlate-with-disease-activity
#10
Saida Farah Issa, Anne Friesgaard Christensen, Hanne M Lindegaard, Merete L Hetland, Kim Hørslev-Petersen, Kristian Stengaard-Pedersen, Bo Jannik Ejbjerg, Tine Lottenburger, Torkell Ellingsen, Jens Kristian Pedersen, Kirsten Junker, Anders Svendsen, Ulrik Tarp, Mikkel Østergaard, Peter Junker
Galectin-3 has been suggested as a pro-inflammatory mediator in animal arthritis and rheumatoid arthritis (RA). We aimed to study the serum level of galectin-3 in patients with newly diagnosed RA and associations with disease profile, Magnetic Resonance Imaging (MRI) findings and seromarkers of synovial matrix inflammation. One hundred and sixty DMARD naïve patients newly diagnosed with RA were included (CIMESTRA study). Clinical, serological and imaging data were recorded before treatment and at 6 weeks, 3 and 12 months...
October 9, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28987865/hyaluronan-synthase-3-promotes-plaque-inflammation-and-atheroprogression
#11
Susanne Homann, Maria Grandoch, Lena S Kiene, Yanina Podsvyadek, Kathrin Feldmann, Berit Rabausch, Nadine Nagy, Stefan Lehr, Inga Kretschmer, Alexander Oberhuber, Paul Bollyky, Jens W Fischer
OBJECTIVE: Hyaluronan (HA) is a prominent component of the provisional extracellular matrix (ECM) present in the neointima of atherosclerotic plaques. Here the role of HA synthase 3 (HAS3) in atheroprogression was studied. APPROACH AND RESULTS: It is demonstrated here that HAS isoenzymes 1, -2 and -3 are expressed in human atherosclerotic plaques of the carotid artery. In Apolipoprotein E (Apoe)-deficient mice Has3 expression is increased early during lesion formation when macrophages enter atherosclerotic plaques...
October 5, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28987501/composite-alginate-hyaluronan-sponges-for-the-delivery-of-tranexamic-acid-in-post-extractive-alveolar-wounds
#12
Ovidio Catanzano, Vittoria D'Esposito, Pietro Formisano, Joshua S Boateng, Fabiana Quaglia
The management of wounds in patients on anticoagulant therapy who require oral surgical procedures is problematic and often results in a non-satisfactory healing process. Here we report a method to prepare an advanced dressing able to avoid uncontrolled bleeding by occluding the post-extractive alveolar wounds, and simultaneously, capable of a fast release of tranexamic acid (TA). Composite alginate/hyaluronan (ALG/HA) sponge dressings loaded with TA were prepared by a straightforward internal gelation method followed by a freeze-drying step...
October 5, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28983581/bioinformatics-analysis-of-fibroblasts-exposed-to-tgf%C3%A2-%C3%AE-at-the-early-proliferation-phase-of-wound-repair
#13
Bobin Mi, Guohui Liu, Wu Zhou, Huijuan Lv, Kun Zha, Yi Liu, Qipeng Wu, Jing Liu
The aim of the current study was to identify gene signatures during the early proliferation stage of wound repair and the effect of TGF‑β on fibroblasts and reveal their potential mechanisms. The gene expression profiles of GSE79621 and GSE27165 were obtained from GEO database. Differentially expressed genes (DEGs) were identified using Morpheus and co‑expressed DEGs were selected using Venn Diagram. Gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs were performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) online tool...
September 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28981299/receptor-mediated-attachment-and-uptake-of-hyaluronan-conjugates-by-breast-cancer-cells
#14
Kush Shah, Andrew J Ditto, Douglas Cale Crowder, Jean H Overmeyer, Hossein Tavana, William A Maltese, Yang H Yun
Chemotherapy, a mainstay modality for cancer, is often hindered by systemic toxicity and side effects. With the emergence of nanomedicine, the development of drug therapy has shifted toward targeted therapy. Hyaluronan (HA) is an ideal molecule as a targeted delivery system because many carcinomas overexpress HA receptors. We have conjugated resveratrol, a natural polyphenol, and 2-methyl-1H-indol-3-yl)-1-(4-pyridinyl)-2-propen-1-one (MOMIPP), a chalcone, to HA with the goal of enhancing drug bioavailability and targeting triple negative breast cancers...
October 5, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28978412/layilin-is-critical-for-mediating-hyaluronan-35kda-induced-intestinal-epithelial-tight-junction-protein-zo-1-in-vitro-and-in-vivo
#15
Yeojung Kim, Gail A West, Greeshma Ray, Sean P Kessler, Aaron C Petrey, Claudio Fiocchi, Christine McDonald, Michelle S Longworth, Laura E Nagy, Carol A de la Motte
Tight junction proteins are critical in maintaining homeostatic intestinal permeability. Multiple intestinal inflammatory diseases are correlated with reduced expression of tight junction proteins. We have recently reported that oral treatment of mice with Hyaluronan 35kDa (HA35) increases colonic expression of tight junction protein zonula occludens-1 (ZO-1). Here, we investigate whether HA35 treatment enhances ZO-1 expression by direct interaction with intestinal epithelium in vitro and have identified the HA receptor responsible for HA35-mediated ZO-1 induction in colonic epithelium in vitro and in vivo...
October 1, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28974616/murine-cytomegalovirus-spreads-by-dendritic-cell-recirculation
#16
Helen E Farrell, Kimberley Bruce, Clara Lawler, Martha Oliveira, Rhonda Cardin, Nicholas Davis-Poynter, Philip G Stevenson
Herpesviruses have coevolved with their hosts over hundreds of millions of years and exploit fundamental features of their biology. Cytomegaloviruses (CMVs) colonize blood-borne myeloid cells, and it has been hypothesized that systemic dissemination arises from infected stem cells in bone marrow. However, poor CMV transfer by stem cell transplantation argues against this being the main reservoir. To identify alternative pathways for CMV spread, we tracked murine CMV (MCMV) colonization after mucosal entry. We show that following intranasal MCMV infection, lung CD11c(+) dendritic cells (DC) migrated sequentially to lymph nodes (LN), blood, and then salivary glands...
October 3, 2017: MBio
https://www.readbyqxmd.com/read/28972496/effects-of-exposure-to-radioactive-56mno2-powder-on-hyaluronan-synthase-2-in-the-lungs-of-rats
#17
Y Kairkhanova, A Saimova, D Uzbekov, N Chaizhunusova, N Fujimoto
Manganese-56 (56Mn) is among the predominant radioisotopes produced in soil by neutrons from atomic bombs. Previously, we examined the effects of the internal exposure of this radioisotope in Wistar rats and reported significant pathological changes in the lung and small intestine. In the present study, we focused on its effects on hyaluronan synthase 2 (Has2) gene expression that may related to pathological changes in the lung. Ten-week-old male Wistar rats were exposed to neutron-activated 56MnO2 powder, non-radioactive MnO2 powder or external 60Co γ-rays (2 Gy, whole body)...
September 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28965026/impact-of-a-timed-release-fsh-treatment-from-2-to-6-months-of-age-in-bulls-i-endocrine-and-testicular-development-of-beef-bulls
#18
B R Harstine, L H Cruppe, F M Abreu, A D Rodrigues, C Premanandan, J M DeJarnette, M L Day
In prepubertal males, FSH facilitates Sertoli cell proliferation and testis maturation. The study aimed to determine the effect of an exogenous FSH treatment on hormone secretion and testis development in Angus bulls. Bulls (n = 22) weaned at 53 ± 3.8 days of age were randomized into two treatment groups based on age and pedigree. Beginning at Day 59, bulls were injected im every 3.5 days with either 30 mg FSH (Folltropin-V; NIH-FSH-P1 units) in a 2% hyaluronan solution (FSH-HA, n = 11) or saline (control, n = 11) until Day 167...
September 21, 2017: Theriogenology
https://www.readbyqxmd.com/read/28965025/impact-of-a-timed-release-fsh-treatment-from-2-to-6-months-of-age-in-bulls-ii-endocrinology-puberty-attainment-and-mature-sperm-production-in-holstein-bulls
#19
B R Harstine, L H Cruppe, F M Abreu, A D Rodrigues, J M DeJarnette, M L Day
The use of genomic testing in the cattle industries has renewed an interest in hastening bull puberty. In prepubertal males, FSH facilitates Sertoli cell proliferation and testis maturation. The aim of this study was to determine the effect of prepubertal administration of a timed-release FSH (delivered in a hyaluronan solution) on hormone secretion, puberty attainment, and mature sperm production in Holstein bulls in an AI center. Bulls (n = 29) were randomly assigned to one of two treatment groups based on birth date and pedigree...
September 21, 2017: Theriogenology
https://www.readbyqxmd.com/read/28963863/hemocompatibility-of-hyaluronan-enhanced-linear-low-density-polyethylene-for-blood-contacting-applications
#20
Rachael Simon-Walker, John Cavicchia, David A Prawel, Lakshmi Prasad Dasi, Susan P James, Ketul C Popat
Despite their overall success, different blood-contacting medical devices such as heart valves, stents, and so forth, are still plagued with hemocompatibility issues which often result in the need for subsequent replacement and/or life-long anticoagulation therapy. Consequently, there is a significant interest in developing biomaterials that can address these issues. Polymeric-based materials have been proposed for use in many applications due to their ability to be finely tuned through manufacturing and surface modification to enhance hemocompatibility...
September 30, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
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