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Hsiao-Han Wang, Chen-Chieh Liao, Nan-Haw Chow, Lynn Ling-Huei Huang, Jih-Ing Chuang, Kuo-Chen Wei, Jyh-Wei Shin
Glioblastoma multiforme (GBM) is one of the most malignant and aggressive brain tumors with great amount of hyaluronan (HA) secretion and CD44 overexpression (HA receptor). CD44 has been suggested as a cancer stem cells (CSCs) marker. However, several clinical studies have indicated that CD44low glioma cell exhibit CSCs traits. Additionally, our previous study indicated that more CD44 expression was associated with a better prognosis in GBM patients. To determine whether CD44 is an appropriate marker of glioma stem cells (GSCs), we manipulated CD44 expression using intrinsic (CD44 knockdown, CD44kd) and extrinsic (HA supplement, HA+) methods...
2017: American Journal of Translational Research
Qi Gao, Guangbing Wei, Yunhua Wu, Na Yao, Cancan Zhou, Kai Wang, Kang Wang, Xuejun Sun, Xuqi Li
Although materials and modern surgical techniques have been developed to suppress postoperative adhesions, adhesion formation can still occur, and thus, a novel effective anti-adhesion drug is greatly needed. In the present study, we explored the efficacy of paeoniflorin treatment against postoperative peritoneal adhesions and examined the anti-oxidative stress and anti-inflammatory properties of PE. Forty-eight male Sprague-Dawley rats were randomly divided into 6 groups for the study: the sham, control, hyaluronan and three concentrations (10, 20 and 40 mg/kg/d) paeoniflorin groups...
November 7, 2017: Oncotarget
Natasha McRae, Leonard Forgan, Bryony McNeill, Alex Addinsall, Daniel McCulloch, Chris Van der Poel, Nicole Stupka
In Duchenne muscular dystrophy (DMD), a dysregulated extracellular matrix (ECM) directly exacerbates pathology. Glucocorticoids are beneficial therapeutics in DMD, and have pleiotropic effects on the composition and processing of ECM proteins in other biological contexts. The synthesis and remodelling of a transitional versican-rich matrix is necessary for myogenesis; whether glucocorticoids modulate this transitional matrix is not known. Here, versican expression and processing were examined in hindlimb and diaphragm muscles from mdx dystrophin-deficient mice and C57BL/10 wild type mice...
December 6, 2017: International Journal of Molecular Sciences
Jelena Rnjak-Kovacina, Fengying Tang, John M Whitelock, Megan S Lord
Proteoglycans and their glycosaminoglycans (GAG) are essential for life as they are responsible for orchestrating many essential functions in development and tissue homeostasis, including biophysical properties and roles in cell signaling and extracellular matrix assembly. In an attempt to capture these biological functions, a range of biomaterials are designed to incorporate off-the-shelf GAGs, typically isolated from animal sources, for tissue engineering, drug delivery, and regenerative medicine applications...
December 6, 2017: Advanced Healthcare Materials
Gabriel A Bó, Dragan R Rogan, Reuben J Mapletoft
A single dose protocol of FSH for superstimulation in cattle may improve compliance and superovulatory response. A single subcutaneous (sc) administration of pFSH was efficacious, but response depended on body condition and injection site; the adipose tissue pad behind the shoulder was most efficacious. Inconsistent results in Holsteins were partially overcome by sc administration of 75% of the total pFSH dose behind the shoulder on the first day followed by 25% 48 h later. An alternative would be to combine FSH with polymers that cause it to be released slowly over several days...
September 28, 2017: Theriogenology
Seong Uk Son, Jae-Woo Lim, Taejoon Kang, Juyeon Jung, Eun-Kyung Lim
We suggest a convenient nanoemulsion fabrication method to create hyaluronan (HA)-based nanohydrogels for effective transdermal delivery. First, hyaluronan-conjugated dodecylamine (HA-Do) HA-based polymers to load the lipophilic agents were synthesized with hyaluronan (HA) and dodecylamine (Do) by varying the substitution ratio of Do to HA. The synthetic yield of HA-Do was more than 80% (HA-Do (A): 82.7 ± 4.7%, HA-Do (B): 87.1 ± 3.9% and HA-Do (C): 81.4 ± 4.5%). Subsequently, nanohydrogels were fabricated using the nanoemulsion method...
December 4, 2017: Nanomaterials
Peng Li, Zhuangzhuang Cong, Yong Qiang, Lei Xiong, Li Tang, Yu Zhang, Haiwei Wu, Jun Yi, Hua Jing, Demin Li, Yi Shen
Lymph node metastasis (LNM) is one of the major causes of cancer‑associated morbidity and mortality in patients with lung cancer following radical pulmonary carcinoma resection. The present study aimed to investigate the relationship between the expression of collagen and calcium‑binding epidermal growth factor domain‑containing protein 1 (CCBE1) and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1) in tumor tissue with the clinical prognosis of lung cancer. The present study included 40 patients with lung cancer that underwent pulmonary carcinoma resection, including 10 patients with LNM, and 10 control patients who underwent pulmonary bullae resection...
November 29, 2017: Molecular Medicine Reports
Kiminori Sato
1. There is growing evidence to suggest that the cells in the maculae flavae are tissue stem cells of the human vocal fold and maculae flavae are a candidate for a stem cell niche. 2. The latest research shows that the cells in the human maculae flavae are involved in the metabolism of extracellular matrices that are essential for the viscoelasticity in the human vocal fold mucosa as a vibrating tissue, and considered to be important cells in the growth, development, and aging of the human vocal fold mucosa...
2017: Advances in Experimental Medicine and Biology
M Casale, P Vella, A Moffa, V Grimaldi, L Sabatino, V Rinaldi, M A Lopez, P Baptista, F Salvinelli
Rhinosinusitis is one of the most common inflammatory conditions of the nasal cavity and paranasal sinuses and is one of the most common causes of absence from work and for visits to the family doctor. The treatment strategy in both acute rhinosinusitis (ARS) and chronic rhinosinusitis (CRS) is to reduce the severity of the symptoms, minimize the duration of the disease and prevent complications. Topical therapy has become an important tool in otolaryngologists’ armamentarium for rhinosinusitis treatment. Recently, topical hyaluronic acid (HA), the major component of many extracellular matrices that promotes tissue healing, including activation and moderation of the inflammatory responses, cell proliferation, migration and angiogenesis, has been proposed for ARS and CRS adjuvant tool...
December 4, 2017: Journal of Biological Regulators and Homeostatic Agents
F Abbruzzese, F Basoli, M Costantini, S M Giannitelli, M Gori, P Mozetic, A Rainer, M Trombetta
Hyaluronic acid (HA) is a polyanionic natural polymer occurring as a linear polysaccharide composed of glucuronic acid and N-acetylglucosamine repeats. Hyaluronic acid has a wide range of applications with its excellent physicochemical properties such as biodegradability, biocompatibility, non-toxicity, non-immunogenicity and serves as an excellent tool in biomedical applications such as osteoarthritis surgery, ocular surgery, plastic surgery, tissue engineering and drug delivery. This work provides an overview on hyaluronic acid, its chemistry and biochemistry and its medical applications...
December 4, 2017: Journal of Biological Regulators and Homeostatic Agents
Fuat Kızılay, Barış Altay
Conventional semen analysis solely is not completely adequate to predict pregnancy outcomes. Therefore, advanced sperm function tests have been developed and introduced to clinical practice. These tests use different methods and techniques to evaluate different stages of fertilization steps. In this review, we reported some commonly used sperm function tests: sperm penetration assay, sperm-zona pellucida binding test (hemizona assay), acrosomal reaction test, hyaluronan binding test, hypo-osmotic swelling test, magnetic-activated cell sorting and zeta sperm selection...
December 2017: Turkish Journal of Urology
Marwa Qadri, Sara Almadani, Gregory D Jay, Khaled A Elsaid
Osteoarthritis (OA) is a low-grade chronic inflammatory joint disease. Innate immunity contributes to OA progression, mediated by TLR2 and TLR4. We evaluated the role of cluster determinant 44 (CD44), a transmembrane glycoprotein, in regulating TLR2-linked macrophage activation and resultant proinflammatory responses. TLR2 stimulation was performed on differentiated THP-1 macrophages in the presence or absence of a CD44-specific Ab or hyaluronan (HA). NF-κB nuclear translocation, IL-1 β and TNF-α gene expression, and protein concentrations were determined...
December 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
Philip L Sannes
No abstract text is available yet for this article.
December 2017: American Journal of Respiratory Cell and Molecular Biology
Matthew R Blackburn, Caitlin Hubbard, Volker Kiessling, Yunchen Bi, Brian Kloss, Lukas Tamm, Jochen Zimmer
Hyaluronan (HA) is an acidic high molecular weight cell surface polysaccharide ubiquitously expressed by vertebrates, some pathogenic bacteria, and even viruses. HA modulates many essential physiological processes and is implicated in numerous pathological conditions ranging from autoimmune diseases to cancer. In various pathogens, HA functions as a non-immunogenic surface polymer that reduces host immune responses. It is a linear polymer of strictly alternating glucuronic acid and N-acetylglucosamine units synthesized by HA synthase (HAS), a membrane-embedded family-2 glycosyltransferase...
November 27, 2017: Glycobiology
Zhengliang L Wu, Anthony Person, Matthew Anderson, Barbara Burroughs, Timothy Tatge, Kshitij Khatri, Yonglong Zou, Lianchun Wang, Todd Geders, Joseph Zaia, Robert Sackstein
Heparan sulfate (HS) is a polysaccharide fundamentally important for biologically activities. T/Tn antigens are universal carbohydrate cancer markers. Here, we report the specific imaging of these carbohydrates using a mesenchymal stem cell line and human umbilical vein endothelial cells (HUVEC). The staining specificities were demonstrated by comparing imaging of different glycans and validated by either removal of target glycans, which results in loss of signal, or installation of target glycans, which results in gain of signal...
November 25, 2017: Glycobiology
Ziba Razinia, Paola Castagnino, Tina Xu, Alexandra Vázquez-Salgado, Ellen Puré, Richard K Assoian
Information in the microenvironment guides complex cellular decisions such as whether or not to proliferate and migrate. The effects of soluble extracellular signals on these cellular functions are fairly well understood, but relatively little is known about how the extracellular matrix (ECM), and particularly the mechanical information in the ECM, guides these cellular decisions. Here, we show that CD44, a major receptor for the glycosaminoglycan ECM component hyaluronan, coordinates the motility and proliferative responses to ECM stiffening...
November 28, 2017: Scientific Reports
Nadine Nagy, Adi de la Zerda, Gernot Kaber, Pamela Y Johnson, Kenneth H Hu, Michael J Kratochvil, Koshika Yadava, Wenting Zhao, Yi Cui, Guadalupe Navarro, Justin P Annes, Thomas N Wight, Sarah C Heilshorn, Paul L Bollyky, Manish J Butte
We have identified a novel role for hyaluronan (HA), an extracellular matrix (ECM) polymer, in governing the mechanical properties of inflamed tissues. We recently reported that insulitis in type 1 diabetes (T1D) of mice and humans is preceded by intra-islet accumulation of HA, a highly hygroscopic polymer. Using the DORmO double transgenic (DO11.10 x RIPmOVA) mouse model of T1D, we asked whether autoimmune insulitis was associated with changes in the stiffness of islets. To measure islet stiffness, we used atomic force microscopy (AFM) and developed a novel "bed of nails"-like approach that uses quartz glass nanopillars to anchor islets, solving a long-standing problem of keeping tissue-scale objects immobilized while performing AFM...
November 28, 2017: Journal of Biological Chemistry
Antonietta Stellavato, Marcella La Noce, Luisana Corsuto, Anna Virginia Adriana Pirozzi, Mario De Rosa, Gianpaolo Papaccio, Chiara Schiraldi, Virginia Tirino
BACKGROUND/AIMS: Adipose-derived Stem Cells (ASCs) are used in Regenerative Medicine, including fat grafting, recovery from local tissue ischemia and scar remodeling. The aim of this study was to evaluate hyaluronan based gel effects on ASCs differentiation and proliferation. METHODS: Comparative analyses using high (H) and low (L) molecular weight hyaluronans (HA), hyaluronan hybrid cooperative complexes (HCCs), and high and medium cross-linked hyaluronan based dermal fillers were performed...
November 27, 2017: Cellular Physiology and Biochemistry
Liyang Shi, Yu Zhang, Dmitri Ossipov
In situ cross-linked hyaluronan (HA) hydrogels with different capacities for biomineralization were prepared and their enzymatic degradation was monitored. Covalent incorporation of bisphosphonates (BPs) into HA hydrogel results in the increased stiffness of the hydrogel in comparison with the unmodified HA hydrogel of the same cross-linking density. The rate of enzymatic degradation of HABP hydrogel was significantly lower than the rate of degradation of control HA hydrogel in vitro. This effect is observed only in the presence of calcium ions that strongly bind to the matrix-anchored BP groups and promote further mineralization of the matrix...
November 27, 2017: Biopolymers
Luiz F Ferrari, Eugen V Khomula, Dioneia Araldi, Jon D Levine
We studied, in male Sprague-Dawley rats, the role of the cognate hyaluronan (HA) receptor, CD44 signaling in the anti-hyperalgesia induced by high molecular weight hyaluronan (HMWH). Low molecular weight hyaluronan (LMWH) acts at both peptidergic and non-peptidergic nociceptors to induce mechanical hyperalgesia that is prevented by intrathecal oligodeoxynucleotide (ODN) antisense to CD44 mRNA, which also prevents hyperalgesia induced by a CD44 receptor agonist, A6. Ongoing LMWH and A6 hyperalgesia is reversed by HMWH...
November 23, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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