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https://www.readbyqxmd.com/read/29050153/-study-on-the-differentiation-of-rat-bone-marrow-mesenchymal-stem-cells-into-chondrocytes-induced-by-tgf-beta-3
#1
D B Liu, X S Han, W L Huang, J Deng, R F She
Objective: To explore the methods for the differentiation of rat bone marrow mesenchymal stem cells (BMMSCs) into chondrocytes induced by transforming growth factor (TGF)-beta 3 in vitro, and search for a reliable seed cell for the regeneration and repair of articular cartilage in osteoarthritis. Methods: SD rat femoral and tibial BMMSCs were cultured by the whole bone marrow adherent method, and then the purity was identified by flow cytometry. P3 generation cells were induced and differentiated into chondrocytes by the induction of differentiation medium containing TGF-beta 3, and cell chondrogenic differentiation ability at different induction time points was detected...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29048704/hydroxyapatite-decreases-cytotoxicity-of-a-glass-ionomer-cement-by-calcium-fluoride-uptake-in-vitro
#2
Casiano Del Angel-Mosqueda, Rene Hernandez-Delgadillo, Osvelia E Rodríguez-Luis, María T Ramírez-Rodríguez, Silvia Munguía-Moreno, Norma V Zavala-Alonso, Juan M Solís-Soto, María A A Nakagoshi-Cepeda, Rosa I Sánchez-Nájera, Sergio E Nakagoshi-Cepeda, Claudio Cabral-Romero
BACKGROUND: Glass ionomer cements (GICs) are widely used in dentistry because of their remineralizing and cariostatic potential induced by fluoride. In vitro studies have reported cell toxicity triggered by GICs; however, the influence of hydroxyapatite (HAp) must be considered. The aim of this study was to evaluate the effect of HAp in decreasing the cytotoxicity of the GIC 3M Vitrebond in vitro. METHODS: Samples of 3M Vitrebond (powder, liquid and light-cured) were incubated in Dulbecco's modified Eagle's medium-Ham's F12 (DMEM-F12) for 24 hours at 37°C...
October 14, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29048187/electronic-cigarette-user-plasma-nicotine-concentration-puff-topography-heart-rate-and-subjective-effects-influence-of-liquid-nicotine-concentration-and-user-experience
#3
Marzena Hiler, Alison Breland, Tory Spindle, Sarah Maloney, Thokozeni Lipato, Nareg Karaoghlanian, Alan Shihadeh, Alexa Lopez, Carolina Ramôa, Thomas Eissenberg
Electronic cigarette (ECIG) nicotine delivery and other effects may depend on liquid nicotine concentration and user experience. This study is the first to systematically examine the influence of ECIG liquid nicotine concentration and user experience on nicotine delivery, heart rate, puff topography, and subjective effects. Thirty-three ECIG-experienced individuals and 31 ECIG-naïve cigarette smokers completed 4 laboratory conditions consisting of 2, 10-puff bouts (30-sec interpuff interval) with a 3.3-V ECIG battery attached to a 1...
October 2017: Experimental and Clinical Psychopharmacology
https://www.readbyqxmd.com/read/29046797/gastrointestinal-tract-like-muscular-walls-in-ovarian-mature-cystic-teratomas-a-histopathological-study
#4
Susumu Matsukuma, Oh Takahashi, Yoshitaka Utsumi, Kosuke Miyai, Hiroaki Takeo
To elucidate the frequency and histopathological features of gastrointestinal (GI) tract-like muscular walls developing in ovarian mature cystic teratoma (MCT), the present study examined 149 MCTs surgically removed from 126 females, including 23 bilateral cases. GI tract-like muscular walls were identified in 9 (7.1%) cases, and were accompanied by mucosa in 5 cases, muscularis mucosae in 5 cases, serosa in 5 cases and all of these components in 3 cases. The mean size of the GI tract-like structures was 0...
October 2017: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29046788/a-rare-case-of-dedifferentiated-liposarcoma-of-the-sinonasal-cavity-a-case-report
#5
Masaru Miyazaki, Mikiko Aoki, Satoru Oba, Toshifumi Sakata, Takashi Nakagawa, Kazuki Nabeshima
Sarcoma is an uncommon histopathological presentation of sinonasal tumors, comprising ~15% of all cases; liposarcoma is particularly uncommon. An analysis of the available medical literature revealed no prior reports of dedifferentiated liposarcoma (DDLPS) of the sinonasal cavity. This case report presents a rare case of DDLPS of the sinonasal cavity. A 40-year old six-week pregnant female was admitted with a left nasal obstruction. Endoscopic evaluation of the left nasal cavity revealed a polypoid lesion. A computed tomography scan indicated a mass invading the left nasal cavity, maxillary sinus and anterior ethmoid sinus with focal destruction of the surrounding bone...
October 2017: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29046436/caenorhabditis-elegans-bub-3-and-san-1-mad3-spindle-assembly-checkpoint-components-are-required-for-genome-stability-in-response-to-treatment-with-ionizing-radiation
#6
Simone Bertolini, Bin Wang, Bettina Meier, Ye Hong, Anton Gartner
Relatively little is known about the crosstalk between the spindle assembly checkpoint and the DNA damage response, especially in multicellular organisms. We performed a Caenorhabditis elegans forward genetic screen to uncover new genes involved in the repair of DNA damage induced by ionizing radiation. We isolated a mutation, gt2000 which confers hypersensitivity to ionizing radiation and showed that gt2000 introduces a premature stop in bub-3 BUB-3 is a key component of the spindle assembly checkpoint. We provide evidence that BUB-3 acts during development and in the germline; irradiated bub-3(gt2000) larvae are developmentally retarded and form abnormal vulvae...
October 18, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29046399/dynamic-localization-of-a-yeast-development-specific-pp1-complex-during-prospore-membrane-formation-is-dependent-on-multiple-localization-signals-and-complex-formation
#7
Tsuyoshi S Nakamura, Yumi Numajiri, Yuuya Okumura, Junji Hidaka, Takayuki Tanaka, Ichiro Inoue, Yasuyuki Suda, Tetsuo Takahashi, Hideki Nakanishi, Xiao-Dong Gao, Aaron M Neiman, Hiroyuki Tachikawa
During the developmental process of sporulation in Saccharomyces cerevisiae, membrane structures called prospore membranes are formed de novo, expand, extend, round up, and finally become plasma membranes of the spores. GIP1 encodes a regulatory/targeting subunit of protein phosphatase type 1 that is required for sporulation. Gip1 recruits the catalytic subunit Glc7 to septin structures that form along the prospore membrane; however, the molecular basis of its localization and function is not fully understood...
October 18, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29046344/kinetochore-microtubule-interactions-in-chromosome-segregation-lessons-from-yeast-and-mammalian-cells
#8
REVIEW
Geethu Emily Thomas, Marira R Renjith, Tapas K Manna
Chromosome congression and segregation require robust yet dynamic attachment of the kinetochore with the spindle microtubules. Force generated at the kinetochore-microtubule interface plays a vital role to drive the attachment, as it is required to move chromosomes and to provide signal to sense correct attachments. To understand the mechanisms underlying these processes, it is critical to describe how the force is generated and how the molecules at the kinetochore-microtubule interface are organized and assembled to withstand the force and respond to it...
October 18, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29046339/a-checkpoint-independent-mechanism-delays-entry-into-mitosis-after-uv-irradiation
#9
Christiane Rothe, Gro Elise Rødland, Silje Anda, Vilte Stonyte, Erik Boye, Sandra Lopez-Aviles, Beáta Grallert
When cells are exposed to stress they delay entry into mitosis. The most extensively studied mechanism behind this delay is the DNA-damage-induced G2/M checkpoint. Here, we show the existence of an additional stress-response pathway which is independent of the classic ATR/Rad3-dependent checkpoint. This novel mechanism delays entry mitosis independent of the spindle assembly checkpoint and the mitotic kinases Fin1, Ark1 and Plo1. The pathway delays activation of the mitotic CDK, Cdc2, after UV irradiation. Furthermore, we demonstrate that translation of the mitotic cyclin Cdc13 is selectively downregulated after UV-irradiation and we propose that this downregulation of Cdc13 contributes to the delayed activation of Cdc2 and the delayed mitosis...
October 18, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29046055/topographic-distribution-pattern-of-morphologically-different-g-cells-in-the-murine-antral-mucosa
#10
Claudia Frick, Hanna Luisa Martin, Johanna Bruder, Kerstin Lang, Heinz Breer
Gastrin-secreting enteroendocrine cells (G cells) in the antrum play an important role in the regulation of gastric secretion, gastric motility and mucosal cell proliferation. Recently we have uncovered the existence of two subpopulations of G cells with pivotally different morphology and a distinct localization in the antral invaginations; the functional implications of the different G cell types are still elusive. In this study a transgenic mouse line in which EGFP is expressed under the control of a gastrin promoter was used to elucidate the distribution pattern of the two G cell types throughout the different regions of the antrum...
September 19, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29042532/three-dimensional-context-rather-than-nls-amino-acid-sequence-determines-importin-%C3%AE-subtype-specificity-for-rcc1
#11
Rajeshwer S Sankhala, Ravi K Lokareddy, Salma Begum, Ruth A Pumroy, Richard E Gillilan, Gino Cingolani
Active nuclear import of Ran exchange factor RCC1 is mediated by importin α3. This pathway is essential to generate a gradient of RanGTP on chromatin that directs nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. Here we identify the mechanisms of importin α3 selectivity for RCC1. We find this isoform binds RCC1 with one order of magnitude higher affinity than the generic importin α1, although the two isoforms share an identical NLS-binding groove. Importin α3 uses its greater conformational flexibility to wedge the RCC1 β-propeller flanking the NLS against its lateral surface, preventing steric clashes with its Armadillo-core...
October 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/29042479/retraction-eya1-controls-cell-polarity-spindle-orientation-cell-fate-and-notch-signaling-in-distal-embryonic-lung-epithelium-development-doi-10-1242-dev-058479
#12
Ahmed Hk El-Hashash, Gianluca Turcatel, Denise Al Alam, Sue Buckley, Hiroshi Tokumitsu, Saverio Bellusci, David Warburton
No abstract text is available yet for this article.
October 15, 2017: Development
https://www.readbyqxmd.com/read/29042221/development-of-photoaffinity-derivatives-of-the-antitumor-macrolide-aplyronine-a-a-ppi-inducer-between-actin-and-tubulin
#13
Masaki Kita, Kota Yamagishi, Kota Tsuchiya, Yu Seguchi, Hiroki Nakane, Hideo Kigoshi
The antitumor and actin-depolymerizing marine macrolide aplyronine A (ApA) synergistically binds to tubulin in association with actin, and prevents spindle formation and mitosis. While the crystal structure of the actin ApA complex was solved in 2006, its interaction with the tubulin heterodimer has not been clarified. To investigate the binding modes of ApA as a unique protein-protein interaction (PPI)-inducer between these two cytoskeletal proteins, we prepared its photoaffinity acetylene and fluorescent derivatives with the aid of molecular modeling studies for probe design...
October 4, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29040814/inhibiting-polo-like-kinase-1-plk1-enhances-radiosensitization-via-modulating-dna-repair-proteins-in-non-small-cell-lung-cancer
#14
Da Yao, Peigui Gu, Youyu Wang, Weibin Luo, Huiliang Chi, Jianjun Ge, Youhui Qian
To assure the faithful chromosome segregation, cells make use of the spindle assembly checkpoint (SAC), which can be activated in aneuploidy cancer cells. In this study, the efficacies of inhibiting Polo-like kinase 1 (PLK1) on the radiosensitization of non-small cell lung cancer (NSCLC) cells were studied. Clonogenic survival assay was performed to identify the effects of the PLK1 inhibitor on radiosensitivity within NSCLC cells. Mitotic catastrophe assessment was used to measure the cell death and γH2AX foci were utilized to assess the DNA double-strand breaks (DSB)...
October 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29040706/inhibition-of-survivin-expression-after-using-oxaliplatin-and-vinflunine-to-induce-cytogenetic-damage-in-vitro-in-lymphocytes-from-colon-cancer-patients-and-healthy-individuals
#15
Amal A A Alotaibi, Mojgan Najafzadeh, Justin D Davies, Adolf Baumgartner, Diana Anderson
Chemotherapy drugs usually inflict a lethal dose to tumour cells with the consequence that these cells are being killed by cell death. However, each round of chemotherapy also causes damage to normal somatic cells. The DNA cross-linking agent oxaliplatin (OXP), which causes DNA double-strand breaks, and vinflunine (VFN), which disrupts the mitotic spindle, are two of these chemotherapy drugs which were evaluated in vitro using peripheral lymphocytes from colorectal cancer patients and healthy individuals to determine any differential response...
October 4, 2017: Mutagenesis
https://www.readbyqxmd.com/read/29040603/mitosis-specific-acetylation-tunes-ran-effector-binding-for-chromosome-segregation
#16
Xiaoling Bao, Heng Liu, Xing Liu, Ke Ruan, Yonghui Zhang, Zhiyong Zhang, Qi Hu, Ying Liu, Saima Akram, Jiahai Zhang, Qingguo Gong, Wenwen Wang, Xiao Yuan, Jian Li, Lingli Zhao, Zhen Dou, Ruijun Tian, Xuebiao Yao, Jihui Wu, Yunyu Shi
Stable transmission of genetic information during cell division requires faithful mitotic spindle assembly and chromosome segregation. The Ran GTPase plays a key role in mitotic spindle assembly. However, how the generation of a chemical gradient of Ran-GTP at the spindle is coupled to mitotic post-translational modifications has never been characterized. Here, we solved the complex structure of Ran with the nucleotide release factor Mog1 and delineated a novel mitosis-specific acetylation-regulated Ran-Mog1 interaction during chromosome segregation...
October 6, 2017: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29034521/sleep-spindle-detection-using-multivariate-gaussian-mixture-models
#17
Chanakya Reddy Patti, Thomas Penzel, Dean Cvetkovic
In this research study we have developed a clustering-based automatic sleep spindle detection method that was evaluated on two different databases. The databases consisted of 20 all-night polysomnograph recordings. Past detection methods have been based on subject-independent and some subject-dependent parameters, such as fixed or variable thresholds to identify spindles. Using a multivariate Gaussian mixture model clustering technique, our algorithm was developed to use only subject-specific parameters to detect spindles...
October 16, 2017: Journal of Sleep Research
https://www.readbyqxmd.com/read/29033760/colonic-ganglioneuroma-a-rare-finding-during-colorectal-cancer-screening
#18
Emmanuel Ofori, Mel Ona, Daryl Ramai, Tiangui Huang, Philip Xiao, Madhavi Reddy
Ganglioneuromas are very rare clinical entities, and their occurrence in the large bowel lays further emphasis on their rarity. Ganglioneuromas are benign tumors of undifferentiated neural crest cells. Their clinical presentation is mostly asymptomatic, and if any symptoms are present at all, they are usually nonspecific, with excellent prognosis. We report an asymptomatic, 65-year-old male with a solitary ascending colonic polyp found on screening colonoscopy. Histology revealed benign polypoid spindle-cell proliferation as well as S100 reactivity, consistent with ganglioneuroma...
May 2017: Case Reports in Gastroenterology
https://www.readbyqxmd.com/read/29031741/further-evidences-for-sleep-instability-and-impaired-spindle-delta-dynamics-in-schizophrenia-a-whole-night-polysomnography-study-with-neuroloop-gain-and-sleep-cycle-analysis
#19
Arun Sasidharan, Sunil Kumar, Ajay Kumar Nair, Ammu Lukose, Vrinda Marigowda, John P John, Bindu M Kutty
OBJECTIVE: Sleep offers a unique window into the brain dysfunctions in schizophrenia. Many past sleep studies have reported abnormalities in both macro-sleep architecture (like increased awakenings) as well as micro-sleep-architecture (like spindle deficits) in patients with schizophrenia (PSZ). The present study attempts to replicate previous reports of macro- and micro-sleep-architectural abnormalities in schizophrenia. In addition, the study also examined sleep-stage changes and spindle-delta dynamics across sleep-cycles to provide further evidence in support of the dysfunctional thalamocortical mechanisms causing sleep instability and poor sleep maintenance associated with schizophrenia pathophysiology...
October 2017: Sleep Medicine
https://www.readbyqxmd.com/read/29031103/kaposi-s-sarcoma-herpesvirus-induced-endothelial-cell-reprogramming-supports-viral-persistence-and-contributes-to-kaposi-s-sarcoma-tumorigenesis
#20
REVIEW
Silvia Gramolelli, Päivi M Ojala
Kaposi's sarcoma (KS) is an endothelial tumor causally linked to Kaposi's sarcoma herpesvirus (KSHV) infection. At early stages of KS, inflammation and aberrant neoangiogenesis are predominant, while at late stages the disease is characterized by the proliferation of KSHV-infected spindle cells (SC). Since KSHV infection modifies the endothelial cell (EC) identity, the origin of SCs remains elusive. Yet, pieces of evidence indicate the lymphatic origin. KSHV-infected ECs display increased proliferative, angiogenic and migratory capacities which account for KS oncogenesis...
October 11, 2017: Current Opinion in Virology
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