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https://www.readbyqxmd.com/read/28214340/molecular-tiling-on-the-surface-of-a-bacterial-spore-the-exosporium-of-the-bacillus-anthracis-cereus-thuringiensis-group
#1
Cassandra Terry, Shuo Jiang, David S Radford, Wan Qiang, Svetomir Tzokov, Anne Moir, Per A Bullough
Bacteria of the genera Bacillus and Clostridium form highly resistant spores, which in the case of some pathogens act as the infectious agents. An exosporium forms the outermost layer of some spores; it plays roles in protection, adhesion, dissemination, host targeting in pathogens, and germination control. The exosporium of the Bacillus cereus group, including the anthrax pathogen, contains a 2D-crystalline basal layer, overlaid by a hairy nap. BclA and related proteins form the hairy nap, and require ExsFA (BxpB) for their localisation on the basal layer...
February 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28213140/a-novel-sensor-for-determination-of-naproxen-based-on-change-in-localized-surface-plasmon-peak-of-functionalized-gold-nanoparticles
#2
Javad Khodaveisi, Ali Mohammad Haji Shabani, Shayessteh Dadfarnia, Dariush Saberi
A highly selective and sensitive colorimetric sensor for the determination of naproxen (NAP) based on the aggregation of the thiolated β-cyclodextrin (Tβ-CD) functionalized gold nanoparticles (Tβ-CD-Au NPs) in the presence of NAP and Zn(2+)is described. The hydrophobic end of NAP interacts with the immobilized Tβ-CD on the Au NPs and forms the complex of Tβ-CD:NAP while the Zn(2+) ions form a 1:2 complex of (NAP)2Zn with the carboxyl groups of NAP resulting in the aggregation of functionalized gold nanoparticles...
February 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28210707/assessing-the-safety-of-physician-directed-nurse-administered-propofol-sedation-in-low-risk-patients-undergoing-endoscopy-and-colonoscopy
#3
Dharshan Sathananthan, Edward Young, Garry Nind, Biju George, Angelie Ashby, Sharon Drummond, Kasia Redel, Neville Green, Rajvinder Singh
Background and study aims Physician-directed nurse-administered balanced propofol sedation (PhD NAPS) in patients undergoing endoscopy and/or colonoscopy is being increasingly utilized worldwide. However, this method of sedation is not universally employed in Australian hospitals due to concerns surrounding its safety. The aim of this study was to assess the safety of PhD NAPS in low-risk patients undergoing endoscopy and/or colonoscopy. Patients and methods This study was conducted at a single tertiary teaching hospital in Adelaide, Australia...
February 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28209525/stromal-vascular-fraction-shows-robust-wound-healing-through-high-chemotactic-and-epithelialization-property
#4
Dong-Sic Chae, Seongho Han, Mina Son, Sung-Whan Kim
BACKGROUND: Although human stromal vascular fraction (SVF) has been regarded as an attractive stem cell source, its therapeutic mechanism in wound healing has not been fully elucidated. AIMS: In this study, we investigated the molecular characteristics and therapeutic property of SVF for wound healing. METHODS: Microarray data showed that SVF cells are enriched with a higher level of wound healing or epithelium development-related genes and micro RNA...
February 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28198510/letter-partial-diallel-crosses-for-predicting-yield-of-semiexotic-maize-populations
#5
C M Silva, J B Miranda Filho, U C Mendes, E F Reis
In Brazil, the total production of maize has increased continuously due to the advances in technology but also to the new frontiers for the maize crop, including the expansion of the second crop (safrinha; usually maize after soybean). Therefore, the exploitation of new sources of germplasm seems to be imperative to attend the actual and future demands for modern cultivars adapted to the greatly variable environments available to the maize crop. The present study was based on a partial diallel (intergroup) cross to provide quantitative information on the potential of two groups of varieties (NAP - exotics and HG - local) and their combining abilities to direct the synthesis of new populations to be used in hybrid crosses or as a base for breeding programs...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28197142/home-made-cost-effective-preservation-buffer-is-a-better-alternative-to-commercial-preservation-methods-for-microbiome-research
#6
Sebastian Menke, Mark A F Gillingham, Kerstin Wilhelm, Simone Sommer
The investigation of wildlife gastrointestinal microbiomes by next-generation sequencing approaches is a growing field in microbial ecology and conservation. Such studies often face difficulties in sample preservation if neither freezing facilities nor liquid nitrogen (LQN) are readily available. Thus, in order to prevent microbial community changes because of bacterial growth after sampling, preservation buffers need to be applied to samples. However, the amount of microbial community variation attributable to the different preservation treatments and potentially affecting biological interpretation is hardly known...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28194142/respiratory-ammonification-of-nitrate-coupled-to-anaerobic-oxidation-of-elemental-sulfur-in-deep-sea-autotrophic-thermophilic-bacteria
#7
Galina B Slobodkina, Andrey V Mardanov, Nikolai V Ravin, Anastasia A Frolova, Nikolay A Chernyh, Elizaveta A Bonch-Osmolovskaya, Alexander I Slobodkin
Respiratory ammonification of nitrate is the microbial process that determines the retention of nitrogen in an ecosystem. To date, sulfur-dependent dissimilatory nitrate reduction to ammonium has been demonstrated only with sulfide as an electron donor. We detected a novel pathway that couples the sulfur and nitrogen cycles. Thermophilic anaerobic bacteria Thermosulfurimonas dismutans and Dissulfuribacter thermophilus, isolated from deep-sea hydrothermal vents, grew autotrophically with elemental sulfur as an electron donor and nitrate as an electron acceptor producing sulfate and ammonium...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28189026/mass-spectrometry-based-in%C3%A2-vitro-assay-investigations-on-the-transformation-of-pharmaceutical-compounds-by-oxidative-enzymes
#8
Lara F Stadlmair, Thomas Letzel, Jörg E Drewes, Johanna Graßmann
The ubiquitous presence of trace organic chemicals in wastewater and surface water leads to a growing demand for novel removal technologies. The use of isolated enzymes has been shown to possess the capability for a targeted application but requires a clearer mechanistic understanding. In this study, the potential of peroxidase from horseradish (HRP) and laccase from Pleurotus ostreatus (LccPO) to transform selected trace organic chemicals was studied using mass spectrometry (MS)-based in vitro enzyme assays...
February 2, 2017: Chemosphere
https://www.readbyqxmd.com/read/28184398/in-situ-study-of-oxidation-states-of-platinum-nanoparticles-on-a-polymer-electrolyte-fuel-cell-electrode-by-near-ambient-pressure-hard-x-ray-photoelectron-spectroscopy
#9
Yasumasa Takagi, Heng Wang, Yohei Uemura, Takahiro Nakamura, Liwei Yu, Oki Sekizawa, Tomoya Uruga, Mizuki Tada, Gabor Samjeské, Yasuhiro Iwasawa, Toshihiko Yokoyama
We performed in situ hard X-ray photoelectron spectroscopy (HAXPES) measurements of the electronic states of platinum nanoparticles on the cathode electrocatalyst of a polymer electrolyte fuel cell (PEFC) using a near ambient pressure (NAP) HAXPES instrument having an 8 keV excitation source. We successfully observed in situ NAP-HAXPES spectra of the Pt/C cathode catalysts of PEFCs under working conditions involving water, not only for the Pt 3d states with large photoionization cross-sections in the hard X-ray regime but also for the Pt 4f states and the valence band with small photoionization cross-sections...
February 10, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28182742/interactive-effects-of-visuomotor-perturbation-and-an-afternoon-nap-on-performance-and-the-flow-experience
#10
Kosuke Kaida, Yoshihiro Itaguchi, Sunao Iwaki
The present study was designed (1) to clarify the relationship between the flow experience and improvements in visuomotor skills, (2) to examine the effects of rotating the axis of a computer mouse on visuomotor skills, and (3) to investigate the effects of sleep for improving visuomotor skills. Participants (N = 18) responded to Perturbation and nap (PER+Nap), No-perturbation and nap (NoPER+Nap) and Perturbation and rest (PER+Rest) conditions. In the PER+Nap condition, participants conducted a visuomotor tracking task using a computer mouse, which was accompanied by perturbation caused by rotating the axis of their mouse...
2017: PloS One
https://www.readbyqxmd.com/read/28181718/segmented-sleep-in-a-nonelectric-small-scale-agricultural-society-in-madagascar
#11
David R Samson, Melissa B Manus, Andrew D Krystal, Efe Fakir, James J Yu, Charles L Nunn
OBJECTIVES: We studied sleep in a rural population in Madagascar to (i) characterize sleep in an equatorial small-scale agricultural population without electricity, (ii) assess whether sleep is linked to noise levels in a dense population, and (iii) examine the effects of experimentally introduced artificial light on sleep timing. METHODS: Using actigraphy, sleep-wake patterns were analyzed for both daytime napping and nighttime wakefulness in 21 participants for a sum total of 292 days...
February 9, 2017: American Journal of Human Biology: the Official Journal of the Human Biology Council
https://www.readbyqxmd.com/read/28167162/disparate-response-to-microoxia-and-nitrogen-oxides-of-the-bradyrhizobium-japonicum-napedabc-nirk-and-norcbqd-denitrification-genes
#12
Emilio Bueno, Eloy F Robles, María J Torres, Tino Krell, Eulogio J Bedmar, María J Delgado, Socorro Mesa
Expression of the Bradyrhizobium japonicum napEDABC, nirK and norCBQD denitrification genes requires low oxygen (O2) tension and nitrate (NO3(-)), through a regulatory network comprised of two coordinated cascades, FixLJ-FixK2-NnrR and RegSR-NifA. To precisely understand how these signals are integrated in the FixLJ-FixK2-NnrR circuit, we analyzed β-Galactosidase activities from napE-lacZ, nirK-lacZ and norC-lacZ fusions and performed analyses of NapC and NorC levels as well as periplasmic nitrate reductase (Nap) activity, in B...
February 3, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28166989/dose-dependent-inhibition-of-uterine-contractility-by-nitric-oxide-a-potential-mechanism-underlying-persistent-breeding-induced-endometritis-in-the-mare
#13
Firdous A Khan, Tracey S Chenier, Coral L Murrant, Robert A Foster, Joanne Hewson, Elizabeth L Scholtz
Nitric oxide (NO) may have a role in persistent breeding-induced endometritis in mares through an inhibitory effect on uterine contractility. The objectives of this study were to test the effect of NO on spontaneous uterine contractility in-vitro and to evaluate whether this effect varied between the longitudinal and circular muscle layers of the uterus. Reproductive tracts were collected from eight euthanized non-pregnant mares (age 4-19 years; body weight 405-530 kg). Transrectal examination of the reproductive tract was performed before euthanasia to evaluate stage of the estrous cycle and presence of any apparent abnormality...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166628/more-than-honey-investigations-on-volatiles-from-monovarietal-honeys-by-using-new-analytical-and-sensory-approaches
#14
Barbara Siegmund, Katharina Urdl, Andrea Jurek, Erich Leitner
Eight monovarietal honeys from dandelion, fir tree, linden tree, chestnut tree, robinia, orange, lavender and rape were investigated with respect to their volatile compounds and their sensory properties. Analysis of the volatile compounds was performed by gas chromatographic tech-niques (one-dimensional GC-MS as well as comprehensive GC x GC-MS). For sensory eva-luation Napping® in combination with ultra flash profiling was applied using sensory experts. For dandelion honey, 34 volatiles compounds are described for the first time to be present in dandelion honey...
February 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28166562/annals-on-being-a-doctor-story-slam-the-valve-and-the-nap
#15
Steven Breecker
No abstract text is available yet for this article.
February 7, 2017: Annals of Internal Medicine
https://www.readbyqxmd.com/read/28148823/spatial-distribution-and-characterization-of-non-apical-progenitors-in-the-zebrafish-embryo-central-nervous-system
#16
Rebecca McIntosh, Joseph Norris, Jon D Clarke, Paula Alexandre
Studies of non-apical progenitors (NAPs) have been largely limited to the developing mammalian cortex. They are postulated to generate the increase in neuron numbers that underlie mammalian brain expansion. Recently, NAPs have also been reported in the retina and central nervous system of non-mammalian species; in the latter, however, they remain poorly characterized. Here, we characterize NAP location along the zebrafish central nervous system during embryonic development, and determine their cellular and molecular characteristics and renewal capacity...
February 2017: Open Biology
https://www.readbyqxmd.com/read/28140528/noncovalent-bonding-of-rgd-and-yigsr-to-an-electrospun-poly-%C3%AE%C2%B5-caprolactone-conduit-through-peptide-self-assembly-to-synergistically-promote-sciatic-nerve-regeneration-in-rats
#17
Lei Zhu, Kai Wang, Teng Ma, Liangliang Huang, Bing Xia, Shu Zhu, Yafeng Yang, Zhongyang Liu, Xin Quan, Kai Luo, Deling Kong, Jinghui Huang, Zhuojing Luo
The nerve conduit with biofunctionalities can regulate neurite outgrowth, as well as the migration, proliferation, and myelination activity of Schwann cells. In the present study, polycaprolactone (PCL) conduits are coated with Naphthalene-phenylalanine-phenylalanine-glycine-arginine-glycine-aspartic (Nap-FFGRGD) and Naphthalene-phenylalanine-phenylalanine-glycine-cysteine-aspartic-proline-glycine-tyrosine-isoleucine-glycine-serine-arginine (Nap-FFGCDPGYIGSR) by self-assembly. In vitro studies demonstrate that arginine-glycine-aspartic (RGD) and tyrosine-isoleucine-glycine-serine-arginine (YIGSR) are capable of synergistically enhancing the ability of PCL to support the adhesion and proliferation of Schwann cells, as well as increasing neurite outgrowth from dorsal root ganglions explants...
January 31, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28133993/a-healthy-diet-optimal-sleep-lifestyle-pattern-is-inversely-associated-with-liver-stiffness-and-insulin-resistance-in-patients-with-nonalcoholic-fatty-liver-disease
#18
Christina N Katsagoni, George V Papatheodoridis, Maria-Vasiliki Papageorgiou, Panagiota Ioannidou, Melanie Deutsch, Alexandra Alexopoulou, Nikolaos Papadopoulos, Elisabeth Fragopoulou, Meropi D Kontogianni
Several lifestyle habits have been described as risk factors for nonalcoholic fatty liver disease (NAFLD). Given that both healthy and unhealthy habits tend to cluster, the aim of this study was to identify lifestyle patterns and explore their potential associations with clinical characteristics of individuals with NAFLD. One hundred and thirty-six consecutive patients with ultrasound-proven NAFLD were included. Diet and physical activity level were assessed through appropriate questionnaires. Habitual night sleep hours and duration of midday naps were recorded...
November 8, 2016: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28133669/remarkable-influence-of-phane-effect-on-the-excited-state-properties-of-cofacially-oriented-coumarins
#19
Arindam Mukhopadhyay, Vijay Kumar Maka, Jarugu Narasimha Moorthy
A comprehensive investigation of the photophysics of a cofacially oriented bis-coumarin based on naphthalene, i.e., Cou-Nap, designed and synthesized to examine the influence of π-electronic communication between the two fluorophores, reveals exceptional excited-state properties. While the anticipated [2 + 2] photocycloaddition is not observed despite the fact that the two reactive coumarin units are at a distance of 3.8 Å, the fluorescence quantum yields and singlet lifetimes in different solvents are found to be remarkably higher when compared to those of the parent coumarin and a mono-coumarin model system, i...
January 30, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28132117/evaluation-of-antimicrobial-persistent-activity-of-alcohol-based-hand-antiseptics-against-bacterial-contamination
#20
R M López-Gigosos, E Mariscal-López, M Gutierrez-Bedmar, A García-Rodriguez, A Mariscal
The purpose of this study was to evaluate persistent activity of three alcohol-based antiseptics widely used in the clinical routine containing chlorhexidine, triclosan or mecetronium after hand disinfection. Four tests were used to evaluate the antimicrobial activity of antiseptics on: (i) resident microbiota; (ii) nutrient agar plates (NAP) subsequently inoculated with a test organism; and transient microbiota acquired by contact with dry hands (iii), or (iv) a wet inert surface that had been artificially contaminated...
January 28, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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