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https://www.readbyqxmd.com/read/28522265/screening-for-bioactive-natural-products-from-a-67-compound-library-of-glycyrrhiza-inflata
#1
Yan Lin, Yi Kuang, Kai Li, Shuang Wang, Wei Song, Xue Qiao, Gulnar Sabir, Min Ye
Licorice shows a variety of pharmacological activities. This work aims to discover bioactive natural products from one botanical source of licorice, Glycyrrhiza inflata. A total of 67 free phenolics were isolated to form a compound library. Based on the bioactivities of licorice, these compounds were screened using cell- or enzyme-based bioassay methods. A total of 11 compounds exhibited potent cytotoxic activities against three human cancer cell lines (HepG2, SW480 and MCF7), while showed little toxicity on human normal cell lines LO2 and HEK293T...
May 6, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28521288/hypermethylation-of-ndn-promotes-cell-proliferation-by-activating-the-wnt-signaling-pathway-in-colorectal-cancer
#2
Yu-Han Hu, Qing Chen, Yan-Xia Lu, Jian-Ming Zhang, Chun Lin, Fan Zhang, Wen-Juan Zhang, Xiao-Min Li, Wei Zhang, Xue-Nong Li
The progression of CRC is a multistep process involving several genetic changes or epigenetic modifications. NDN is a member of the MAGE family, encoding a protein that generally suppresses cell proliferation and acting as a transcriptional repressor. Immunohistochemical staining revealed that the expression of NDN was significantly down-regulated in CRC tissues compared with normal tissues and the down-regulation of NDN in CRC could reflect the hypermethylation of the NDN promoter. Treatment of the CRC cell line SW480 with the demethylating agent 5-Aza-CdR restored the NDN expression level...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28506831/cryab-promotes-the-invasion-and-metastasis-of-colorectal-cancer-via-epithelial-mesenchymal-transition
#3
Chuanbing Shi, Xiaojun Yang, Xiaodong Bu, Ning Hou, Pingsheng Chen
Alpha B-crystallin (CRYAB, HSPB5) is a protein that was first discovered in the lens of the eye. It is a member of the small heat-shock protein family (sHsps). CRYAB functions primarily as a molecular chaperone to prevent the aggregation and degradation of damaged unfolded proteins due to cellular damage resulting from heat shock, radiation, oxidative stress, and other insults, thereby promoting cell survival and preventing apoptosis. In recent years, the role of CRYAB in tumorigenesis, tumor invasion, and metastasis has received increasing attention...
May 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28504924/mrna-expression-of-nuclear-factor-of-activated-t-cells-cytoplasmic-2-nfatc2-and-peroxisome-proliferator-activated-receptor-gamma-pparg-transcription-factors-in-colorectal-carcinoma
#4
Venus Zafari, Shahryar Hashemzadeh, Mohammadali Hosseinpour Feizi, Nasser Pouladi, Leila Rostami Zadeh, Ebrahim Sakhinia
Transcription factors are involved in cell cycle and apoptosis regulation and thus have a key role in the carcinogenesis of different tumors. Nuclear factor of activated T-cells, cytoplasmic 2 (NFATc2) and peroxisome proliferator-activated receptor gamma (PPARG) transcription factors are important in the carcinogenesis of colorectal cancer (CRC). In this study, we examined whether the expression of NFATc2 and PPARG genes is significantly altered during the carcinogenesis of CRC. A total of 47 tumor samples and matched normal tissue margins were collected during surgery from patients with CRC...
May 15, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28504908/mammea-longifolia-planch-and-triana-fruit-extract-induces-cell-death-in-the-human-colon-cancer-cell-line-sw480-via-mitochondria-related-apoptosis-and-activation-of-p53
#5
Chunmei Li, Yoonhwa Jeong, Misook Kim
The methanol extract of Mammea longifolia Planch. and Triana (M. longifolia) fruit was studied for anticancer and apoptotic effects in the SW480 colon cancer cell line. The apoptotic and necrotic effects of M. longifolia were detected by 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide (MTT) and lactate dehydrogenase assays, respectively. One hundred μg/mL of the extract killed ∼82.4% of the cells; however, 2% of the death was related to necrosis. The morphological changes in M. longifolia-stimulated SW480 cells were observed directly by light microscopy...
May 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28503147/an-ethanolic-extract-of-ampelopsis-radix-exerts-anti-colorectal-cancer-effects-and-potently-inhibits-stat3-signaling-in-vitro
#6
Tao Su, Jing-Xuan Bai, Ying-Jie Chen, Xin-Ning Wang, Xiu-Qiong Fu, Ting Li, Hui Guo, Pei-Li Zhu, Yue Wang, Zhi-Ling Yu
Colorectal cancer (CRC) is a leading cause of cancer-related morbidity and mortality worldwide. Signal transducer and activator of transcription 3 (STAT3) signaling is constantly activated in CRC, and has been proposed as a pathogenic factor and a therapeutic target of CRC. Ampelopsis Radix (AR), a traditional Chinese medicinal herb, possesses low toxicity and has long been used clinically for the treatment of cancers including CRC. Some constituents of AR have been reported to exert anti-cancer properties by targeting STAT3...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28502297/-knock-down-of-cadherin-17-inhibits-proliferation-and-promote-apoptosis-in-noscapine-resistant-human-sw480-colon-cancer-cells
#7
Zheng Han, Xiaodong Huang, Meng Liu, Jie Tan, Qingxi Zhu, Yanfen Wang, Yishan Cai, Aifang Chen, Xia Tian
Objective To investigate the effects of cadherin 17 (CDH17) on the proliferation and apoptosis of noscapine-resistant human SW480 colon cancer cells. Methods The level of CDH17 in noscapine-resistant human SW480 colon cancer cells was knocked down by small interfering RNA (siRNA), and the silence was confirmed by Western blotting and real-time quantitative PCR. Transfected SW480 cells were treated with noscapine, and then the proliferation and cell viability of SW480 cells were measured by MTT assay and plate clone formation assay, respectively; the apoptosis of SW480 cells was detected by flow cytometry combined with annexinV-FITC/PI staining; the expressions of poly-ADP-ribose polymerase (PARP) and caspase-3 were determined by Western blotting...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28498834/expression-of-mysm1-is-associated-with-tumor-progression-in-colorectal-cancer
#8
Yongmin Li, Jingwen Li, He Liu, Yanlong Liu, Binbin Cui
Colorectal cancer, the third most common cancer in both men and women, has gradually increased in recent years. MYSM1has been investigated as a regulator of hematopoiesis and lymphocyte development in human. It has been reported that some tumor-related genes were modulated by MYSM1. However, its exact role in cancer development remains unclear. Herein, we aimed to examine the expression level of MYSM1 in tumor tissues and its correlation with clinicopathology and survivals of patients with colorectal cancer (CRC)...
2017: PloS One
https://www.readbyqxmd.com/read/28498393/upregulation-of-circadian-gene-hclock-contribution-to-metastasis-of-colorectal-cancer
#9
Yaping Wang, Ning Sun, Chao Lu, Yibing Bei, Ruizhe Qian, Luchun Hua
Recent studies have shown that disruption of the circadian rhythm was one of the endogenous factors contributing to tumorigenesis of various human malignancies, including colorectal cancer (CRC). However, the roles of circadian genes in the development of CRC are still unexplored. In this study, we investigated the expression pattern and the underlying mechanism of human Clock gene (hClock) in CRC progression. Multiple methods such as qRT-PCR, immunohistochemistry, and western blotting were performed to evaluate the expression pattern of the gene hClock, as well as to observe the changes of angiogenesis-related proteins and EMT-related proteins...
May 8, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28496335/knockdown-of-a-dis3l2-promoter-upstream-long-noncoding-rna-ac105461-1-enhances-colorectal-cancer-stem-cell-properties-in-vitro-by-down-regulating-dis3l2
#10
Wei Liu, Qiang Yu, Jun Ma, Yong Cheng, Hongbo Zhang, Wengguang Luo, Jie Yao, Hongyan Zhang
A large number of studies have identified plentiful long noncoding RNAs (lncRNAs) associated with the development of multiple cancers. Some lncRNAs have also been found to be strongly linked with stem cell properties such as pluripotency and differentiation. However, only in a few cases have cancer stem cell (CSC)-related lncRNAs been studied. Commonly, the expression and function of lncRNAs are associated with adjacent protein coding transcripts. In the present study, we found an lncRNA (AC105461.1), a promoter upstream transcript of DIS3 mitotic control homolog (Saccharomyces cerevisiae)-like 2 (DIS3L2), may be closely connected with "stem cell-like" properties...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28495508/the-effect-of-ala-pdt-under-normoxia-and-cobalt-chloride-cocl2-induced-hypoxia-on-adhesion-molecules-icam-1-vcam-1-secretion-by-colorectal-cancer-cells
#11
Aleksandra Kawczyk-Krupka, Zenon Czuba, Beata Kwiatek, Sebastian Kwiatek, Magdalena Krupka, Karolina Sieroń
BACKGROUND: The most fundamental problem in cancer biology research is to understand the mechanisms of cancer cell resistance to oncological therapies. Literature reports emphasize the important role of adhesion molecules: intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 (ICAM-1 and VCAM-1) in cancer progression and resistance to treatment. Photodynamic therapy (PDT) could become the component of a personalized approach to colorectal cancer, therefore we examined the effects of ALA (δ-aminolevulinic) acid PDT in normoxia and under cobalt chloride (CoCl2)-induced hypoxia on ICAM-1 and VCAM-1 secretion by colorectal cancer cells...
May 8, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28492554/the-novel-long-intergenic-noncoding-rna-ucc-promotes-colorectal-cancer-progression-by-sponging-mir-143
#12
Feng-Ting Huang, Wen-Ying Chen, Zhi-Qiang Gu, Yan-Yan Zhuang, Chu-Qiang Li, Ling-Yun Wang, Juan-Fei Peng, Zhe Zhu, Xin Luo, Yuan-Hua Li, He-Rui Yao, Shi-Neng Zhang
The human genome contains thousands of long intergenic noncoding RNAs (lincRNAs). However, the functional roles of these transcripts and the mechanisms responsible for their deregulation in colorectal cancer (CRC) remain elusive. A novel lincRNA termed upregulated in CRC (UCC) was found to be highly expressed in human CRC tissues and cell lines. UCC levels correlated with lymph node metastasis, Dukes' stage, and patient outcomes. In SW480 and SW620 cells, knockdown of UCC inhibited proliferation, invasion, and cell cycle progression and induced apoptosis in vitro...
May 11, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28492347/the-soy-derived-peptide-vglycin-inhibits-the-growth-of-colon-cancer-cells-in%C3%A2-vitro-and-in%C3%A2-vivo
#13
Chang Gao, Rui Sun, Ya-Rong Xie, An-Li Jiang, Mei Lin, Min Li, Zheng-Wang Chen, Ping Zhang, Honglin Jin, Jue-Ping Feng
Vglycin, a novel natural polypeptide isolated from pea seeds, possesses antidiabetic properties. Our previous studies have shown that Vglycin can induce the differentiation of human colon adenocarcinoma cells. We aimed to determine the anticancer activity of Vglycin against colon cancer cells and to elucidate related apoptosis-inducing mechanisms. Treatment with purified Vglycin significantly reduced growth, viability, and colony formation of CT-26, SW480, and NCL-H716 colon cancer cells in a dose-dependent manner while down-regulating the expression of proliferating cell nuclear antigen...
May 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28491151/methylation-of-diras1-promotes-colorectal-cancer-progression-and-may-serve-as-a-marker-for-poor-prognosis
#14
Ruipan Zheng, Dan Gao, Tao He, Meiying Zhang, Xiaomei Zhang, Enqiang Linghu, Lixin Wei, Mingzhou Guo
BACKGROUND: DIRAS1 is a new member of the Ras gene family. It was described as a potential tumor suppressor in human glioblastomas and esophageal cancer. The role of DIRAS1 in colorectal cancer remains unclear. METHODS: To explore the epigenetic changes and function of DIRAS1 in human colorectal cancer, we studied ten colorectal cancer cell lines and 146 primary colorectal cancer samples and 50 matched adjacent samples using semi-quantitative reverse transcription PCR, immunohistochemistry, methylation-specific PCR and bisulfite sequencing, western blot, flow cytometry, and transwell assays...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28472608/epigenetic-regulation-of-voltage-gated-potassium-ion-channel-molecule-kv1-3-in-mechanisms-of-colorectal-cancer
#15
Tao He, Can Wang, Meiying Zhang, Xiaomei Zhang, Shufang Zheng, Enqiang Linghu, Mingzhou Guo
Colorectal cancer (CRC) is among the leading causes of cancer-related death throughout the world. Aberrant expression of voltage-gated potassium ion channel molecule Kv1.3 has been reported in various cancers. To explore the expression and regulation of Kv1.3 in colorectal cancer, 7 colorectal cancer cell lines and 147 cases of primary colorectal cancer were involved in this study. Kv1.3 was expressed in LOVO and SW480 cells and loss of expression was found in RKO, DLD1, SW620, HCT116, and HT29 cells. Complete methylation was found in RKO, DLD1, SW620, HCT116, and HT29 cells, partial methylation was found in LOVO and SW480 cells...
March 2017: Discovery Medicine
https://www.readbyqxmd.com/read/28468595/sflt-1-inhibits-proliferation-migration-and-invasion-of-colorectal-cancer-sw480-cells-through-vascular-mimicry-formation-suppression
#16
Wang Jinjun, Wang Zhaowei, Li Qiang, Xue Zhijun, Zhang Juanzi, Li Lin, Jiang Guixi
To investigate the effects of soluble fms-like tyrosine kinase-1 on the vascular mimicry formation, proliferation, migration, and invasion of colorectal cancer SW480 cells. The recombinant plasmid pBLAST49-sFLT-1 or pBLAST49 control plasmid was transfected into SW480 cells to obtain hsFLT-1-SW480 or Ctrl-SW480 cells. The three-dimensional model culture, sulforhodamine B assay, scratch assay, and Transwell assay were performed to detect the vascular mimicry formation, proliferation, migration, and invasion of colorectal cancer SW480 cells, respectively...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28468585/suppression-subtractive-hybridization-identified-differentially-expressed-genes-in-colorectal-cancer-microrna-451a-as-a-novel-colorectal-cancer-related-gene
#17
Ke Xu, Yuan-Yuan Zhang, Bin Han, Yang Bai, Yao Xiong, Yi Song, Li-Ming Zhou
To investigate differentially expressed genes regulated by microRNA-451a in colorectal cancer. We detected expression of microRNA-451a in colorectal cancer samples and normal pericarcinous tissues from 68 colorectal cancer patients and the correlation between microRNA-451a and clinical features of these patients. Then, the expression of microRNA-451a in HCT116, SW620, HT29, SW480, and DLD cells was also measured. The suppression subtractive hybridization method was used with two HCT116 cell lines with overexpressing or underexpressing microRNA-451a, respectively...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28455135/sn38-conjugated-hyaluronic-acid-gold-nanoparticles-as-a-novel-system-against-metastatic-colon-cancer-cells
#18
Hosniyeh Hosseinzadeh, Fatemeh Atyabi, Behrang Shiri Varnamkhasti, Reza Hosseinzadeh, Seyed Nasser Ostad, Mohammad Hossein Ghahremani, Rassoul Dinarvand
Combination of chemotherapy and photothermal therapy has been proposed for better treatment of metastatic colon cancer. In this study SN38, a highly potent cytotoxic agent, was conjugated to negatively charged hyaluronic acid (HA), which was deposited on the surface of the positively charged gold nanoparticles via electrostatic interaction. The drug conjugation and its interaction with gold nanoparticles were verified by (1)H NMR and UV-vis spectroscopies, respectively. The prepared SN38-HA gold NPs are negatively charged spherical nanoparticles with an average size of 75±10nm...
April 25, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28454447/inhibitory-effect-and-mechanism-of-exogenous-mammalian-sterile-20-like-kinase-1-on-the-growth-of-human-colorectal-cancer
#19
Jian Wu, Xiaohong Yang, Hongfei Lu, Liqiao Liu, Baohua Xu, Shuangyan Zheng, Bo Yu, Kemin Jie, Fusheng Wan
The present study aimed to observe the inhibitory effect and preliminary mechanism of exogenous mammalian sterile 20-like kinase 1 (MST1) on the growth of colorectal cancer SW480 cells. The SW480 cells were randomly divided into the following groups: Control, empty enhanced green fluorescent protein (EGFP) plasmid (pEGFP-N1), MST1 EGFP plasmid (pEGFP-MST1), 20 µmol/l fluorouracil (5-FU) and pEGFP-MST1 + 5-FU. An MTS colorimetric assay was used to detect cell viability, Hoechst 33342 staining was used to observe cell apoptosis, and western blotting and immunohistochemistry were used to detect the levels of the proteins MST1, yes-associated protein (YAP), phospho-YAP1 (Ser127), p53 and p53 upregulated modulator of apoptosis (PUMA)...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454342/feasibility-and-antitumor-efficacy-in-vivo-of-simultaneously-targeting-glycolysis-glutaminolysis-and-fatty-acid-synthesis-using-lonidamine-6-diazo-5-oxo-l-norleucine-and-orlistat-in-colon-cancer
#20
Diana Cervantes-Madrid, Guadalupe Dominguez-Gomez, Aurora Gonzalez-Fierro, Enrique Perez-Cardenas, Lucia Taja-Chayeb, Catalina Trejo-Becerril, Alfonso Duenas-Gonzalez
The aim of the present study was to investigate in vivo the feasibility and efficacy of the combination of lonidamine (LND), 6-diazo-5-oxo-L-norleucine (DON) and orlistat to simultaneously target glycolysis, glutaminolysis and de novo synthesis of fatty acids, respectively. The doses of LND and DON used in humans were translated to mouse doses (77.7 mg/kg and 145.5 mg/kg, respectively) and orlistat was used at 240 mg/kg. Three schedules of LND, DON and orlistat at different doses were administered by intraperitoneal injection to BALB/c mice in a 21-day cycle (schedule 1: LND, 0...
March 2017: Oncology Letters
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