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Akira Inoue, Tsunekazu Mizushima, Xin Wu, Daisuke Okuzaki, Nanami Kambara, Sho Ishikawa, Jiaqi Wang, Yamin Qian, Haruka Hirose, Yuhki Yokoyama, Ryo Ikeshima, Masayuki Hiraki, Norikatsu Miyoshi, Hidekazu Takahashi, Naotsugu Haraguchi, Taishi Hata, Chu Matsuda, Yuichiro Doki, Masaki Mori, Hirofumi Yamamoto
We previously demonstrated that miR-29b-3p is a hopeful microRNA (miRNA)-based therapies against colorectal cancer (CRC). In this study, we aimed to clarify a value of miR-29b-1-5p as a next generation treatment, especially for KRAS mutant CRC. RT-PCR assay showed that expression of miR-29b-3p was high and its partner strand, miR-29b-1-5p level was only negligible in clinical CRC samples. Mimic-miR-29b-1-5p significantly inhibited proliferation of KRAS mutant CRC cell lines DLD1 and SW480 and KRAS wild type HT29 cells...
March 15, 2018: Molecular Cancer Therapeutics
Nengyi Hou, Zhiyi Guo, Gaoping Zhao, Guiqing Jia, Bin Luo, Xiaogang Shen, Yifeng Bai
MicroRNA-21-3p (miR-21-3p), the passenger strand of pre-mir-21, has been found to be high-expressing in various cancers and to be associated with tumor malignancy, which is proposed as a novel focus in malignant tumors. Colorectal cancer (CRC), currently known as one of the most prevalent malignancy, is a leading cause of cancer death. This study aimed to investigate the key role of miR-21-3p in CRC by inhibiting its expression using transfection with miR-21-3p inhibitors into human CRC HCT116 cells. Results showed that the expression of miR-21-3p was higher than other CRC cells used in the study including Lovo, HT29, Colo320 and SW480 cells, inhibition of which suppressed the proliferation and induced cell cycle arrest in HCT116 cells...
March 14, 2018: Clinical and Experimental Pharmacology & Physiology
Yang Li, Yanhong Duo, Jiangang Bi, Xiaowei Zeng, Lin Mei, Shiyun Bao, Lisheng He, Aijun Shan, Yue Zhang, Xiaofang Yu
Introduction: MicroRNA-155 (miR-155) is an oncogenic microRNA, which is upregulated in many human cancers including colorectal cancer (CRC). Overexpression of miR-155 has been found to regulate several cancer-related pathways, and therefore, targeting miR-155 may be an effective strategy for cancer therapy. However, effective and safe delivery of anti-miR-155 to tumors remains challenging for the clinical applications of anti-miR-155-based therapeutics. Methods: In this study, we explored the expression of miR-155 and the transcription factor nuclear factor kappa B (NF-κB) in CRC tissues and cell lines, and the possible relationship between miR-155 and NF-κB...
2018: International Journal of Nanomedicine
Shanshan Feng, Qiaozhi Zhou, Bo Yang, Qianqian Li, Aiqin Liu, Yingying Zhao, Changqing Qiu, Jun Ge, Huihong Zhai
BACKGROUND: Calcyclin Binding Protein/(Siah-1 interacting protein) (CacyBP/SIP) acts as an oncogene in colorectal cancer. The nuclear accumulation of CacyBP/SIP has been linked to the proliferation of cancer cells. It has been reported that intracellular Ca2+ induces the nuclear translocation of CacyBP/SIP. However, the molecular mechanism of CacyBP/SIP nuclear translocation has yet to be elucidated. The purpose of this study was to test whether the Ca2+-dependent binding partner S100 protein is involved in CacyBP/SIP nuclear translocation in colon cancer SW480 cells...
2018: PloS One
Mu-Lin Liu, Qiao Zhang, Xiao Yuan, Long Jin, Li-Li Wang, Tao-Tao Fang, Wen-Bin Wang
AIM: To investigate the role of long noncoding RNA (lncRNA) RP4 in colorectal cancer. METHODS: Lentivirus-mediated lncRNA RP4 overexpression and knockdown were performed in the colorectal cancer cell line SW480. Cell proliferation, tumor growth, and early apoptosis were evaluated by a cell counting kit-8 assay, an in vivo xenograft tumor model, and annexin V/propidium iodide staining, respectively. Analysis of the lncRNA RP4 mechanism involved assessment of the association of its expression with miR-7-5p and the SH3GLB1 gene...
March 7, 2018: World Journal of Gastroenterology: WJG
Jian-Hai Ding, Zheng-Hui Li, Tao Feng, Ji-Kai Liu
A new tremulane sesquiterpenoid, named irlactam A (1), was isolated from cultures of the fungus Irpex lacteus. The new structure was elucidated by spectroscopic data analysis. The compound was tested for its cytotoxicities on HL-60, SMMC-7721, A-549, MCF-7, and SW480 cells and for its inhibitory activity against isozymes of 11β-hydroxysteroid dehydrogenases (11β-HSD).
March 9, 2018: Natural Product Research
Christoph A Riedl, Michaela Hejl, Matthias H M Klose, Alexander Roller, Michael A Jakupec, Wolfgang Kandioller, Bernhard K Keppler
A series of 11 novel ruthenium(ii) arene complexes [Ru(p-cym)(trzC^N )L]NO3 based on the cycloruthenated 1,2,3-triazole scaffold (trzC^N ) bearing different N- or S-donor leaving groups (L) were prepared. These complexes exhibited strongly diverging pH-dependent stability profiles, but consistently exerted antiproliferative effects in the low micromolar range in three cancer cell lines (A549, SW480, CH1/PA-1). The interaction with biomolecules was correlated to dissociation of the monodentate leaving group...
March 9, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Qiyue Zhang, Jingyuan Wang, Na Li, Zhentao Liu, Zuhua Chen, Zhongwu Li, Yumei Lai, Lin Shen, Jing Gao
This study was designed to investigate the significance of the effect of miR-34a on 5-fluorouracil (5-FU) sensitivity in vitro and in vivo . miR-34a expression in tumor tissues or serum was determined by quantitative polymerase chain reaction. CRC cell lines HCT116 and SW480 were used to evaluate cell viability, cell apoptosis, and the cell cycle using a cell proliferation assay, flow cytometry, and Western blotting, respectively. For the in vivo studies, xenografts derived from SW480 cells were established to assess the antitumor activity between miR-34a and 5-FU...
2018: American Journal of Cancer Research
De Qing Wu, Zi Feng Yang, Ke Jian Wang, Xing Yu Feng, Ze Jian Lv, Yong Li, Zhi Xiang Jian
Colorectal cancer (CRC) is one of the most common malignant cancers and the leading cause of cancer-related deaths in worldwide. Although the monoclonal antibody therapy is prescribed for CRC, the metastasis resistant to therapy is the major cause of death of patients with CRC, which indicating the urgent demands for new therapeutic targets discovery. Aquaporin 8 (AQP8) has been identified alter expressed in several cancers including breast cancer, lung cancer and prostatic carcinoma. Our study demonstrated the functional significance of AQP8 in CRC cells growth and metastasis...
2018: American Journal of Cancer Research
Aleksandra Kawczyk-Krupka, Zenon Czuba, Wojciech Latos, Katarzyna Wasilewska, Thomas Verwanger, Barbara Krammer, Aleksander Sieroń
BACKGROUND: Photodynamic therapy has apart from a direct cytotoxic effect also immunomodulatory properties. The aim of our study was to investigate how photodynamic therapy with 5-aminolevulinic acid (ALA-PDT) in sublethal doses influences the secretion of interleukins 6, 8 and 10 from colon cancer cells in vitro. METHODS: We used two human colon cancer cell lines SW480 and SW620 of different malignancies which were treated with a sublethal PDT protocol. Determination of interleukins was carried out using the Bio- Plex Assay Pro™ kit on the Bio- Plex Suspension Array System...
March 3, 2018: Photodiagnosis and Photodynamic Therapy
Somrudee Reabroi, Arthit Chairoungdua, Rungnapha Saeeng, Teerapich Kasemsuk, Witchuda Saengsawang, Weiming Zhu, Pawinee Piyachaturawat
Hyperactivation of Wnt/β-catenin signaling implicated in oncogenesis of colorectal cancer (CRC) is a potential molecular target for chemotherapy. An andrographolide analogue, 3A.1 (19-tert-butyldiphenylsilyl-8, 17-epoxy andrographolide) has previously been reported to be potently cytotoxic toward cancer cells by unknown molecular mechanisms. The present study explored the anti-cancer activity of analogue 3A.1 on Wnt/β-catenin signaling in colon cancer cells (HT29 cells) which were more sensitive to the others (HCT116 and SW480 cells)...
March 1, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Leila Amininejad, Benoit Charloteaux, Emilie Theatre, Claire Liefferinckx, Julia Dmitrieva, Pierre Hayard, Vincianne Muls, Jean-Marc Maisin, Michael Schapira, Jean-Michel Ghislain, Pierre Closset, Mehdi Talib, Marc Abramowicz, Yukihide Momozawa, Valerie Deffontaine, François Crins, Myriam Mni, Latifa Karim, Nadine Cambisano, Sandra Ornemese, Alessandro Zucchi, Charlotte Minsart, Jacques Deviere, Jean-Pierre Hugot, Martine De Vos, Edouard Louis, Severine Vermeire, Andre Van Gossum, Wouter Coppieters, Jean-Claude Twizere, Michel Georges, Denis Franchimont
BACKGROUND & AIMS: A few rare monogenic primary immunodeficiencies (PID) are characterized by chronic intestinal inflammation that resembles Crohn's disease (CD). We investigated whether 23 genes associated with 10 of these monogenic disorders contain common, low-frequency or rare variants that increase risk for CD. METHODS: Common and low frequency variants in 1 Mb loci centered on the candidate genes were analyzed using meta-data corresponding to genotypes of approximately 17,000 patients with CD or without CD (controls) in Europe...
February 28, 2018: Gastroenterology
Xiang-Yi Yang, Qiao-Rui Liu, Li-Ming Wu, Xu-Lei Zheng, Cong Ma, Ri-Su Na
OBJECTIVE: In order to investigate the effect of secretagogin (SCGN) on colorectal cancer (CRC) cells apoptosis, invasion and migration in vitro. METHODS: Expression of SCGN in CRC tissues and the paired adjacent non-tumorous tissues (n = 36) and four human CRC cell lines (HT29, HCT116, SW480 and SW620) were detected. SW480 cells were transfected with the SCGN overexpression plasmid (eGFP-SCGN), si-SCGN-773, and the corresponding negative controls (NCs). Then, cell-cycle distribution, cell apoptosis, migration, invasion and expression of apoptosis- and metastasis-related proteins were detected...
February 27, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Ajda Coker Gurkan, Elif Damla Arisan, Pinar Obakan Yerlikaya, Halime Ilhan, Narcin Palavan Unsal
PURPOSE: One of the recently developed polyamine (PA) analogues, N1 ,N11 -diethylnorspermine (DENSpm), has been found to act as an apoptotic inducer in melanoma, breast, prostate and colon cancer cells. Also, its potential to induce autophagy has been established. Unfolded protein responses and starvation of amino acids are known to trigger autophagy. As yet, however, the molecular mechanism underlying PA deficiency-induced autophagy is not fully clarified. Here, we aimed to determine the apoptotic effect of DENSpm after autophagy inhibition by 3-methyladenine (3-MA) or siRNA-mediated Beclin-1 silencing in colon cancer cells...
February 28, 2018: Cellular Oncology (Dordrecht)
Binita Das, Beth K Neilsen, Kurt W Fisher, Drew Gehring, Youcai Hu, Deanna J Volle, Hyun Seok Kim, Jamie L McCall, David L Kelly, John B MacMillan, Michael A White, Robert E Lewis
AMPK is a serine threonine kinase composed of a heterotrimer of a catalytic, kinase-containing α and regulatory β and γ subunits. Here we show that individual AMPK subunit expression and requirement for survival varies across colon cancer cell lines. While AMPKα1 expression is relatively consistent across colon cancer cell lines, AMPKα1 depletion does not induce cell death. Conversely, AMPKα2 is expressed at variable levels in colon cancer cells. In high expressing SW480 and moderate expressing HCT116 colon cancer cells, siRNA-mediated depletion induces cell death...
February 28, 2018: Scientific Reports
Hanae Morio, Yuchen Sun, Manami Harada, Hideyuki Ide, Osamu Shimozato, Xujia Zhou, Kenjirou Higashi, Ryuzaburo Yuki, Naoto Yamaguchi, Josefina Piñón Hofbauer, Christina Guttmann-Gruber, Naohiko Anzai, Hidetaka Akita, Kan Chiba, Tomomi Furihata
Cancer-type organic anion transporting polypeptide 1B3 (Ct-OATP1B3) mRNA is a variant isoform of the liver-type OATP1B3. Because Ct-OATP1B3 mRNA shows an excellent cancer-specific expression profile in colorectal cancer (CRC), and that its expression levels are associated with CRC prognosis, it holds the potential to become a useful CRC detection and diagnosis biomarker. While the potential is currently justified only at the tissue level, if existence of Ct-OATP1B3 mRNA in CRC-derived extracellular vesicles (EVs) is validated, the findings could enhance its translational potential as a CRC detection and diagnosis biomarker...
2018: Biological & Pharmaceutical Bulletin
Ping He, Ke Li, Shi-Bao Li, Ting-Ting Hu, Ming Guan, Fen-Yong Sun, Wei-Wei Liu
A-kinase anchor protein 12 (AKAP12; also known as Gravin) functions as a tumor suppressor in several human primary cancers. However, the potential correlation between histone deacetylase 3 (HDAC3) and AKAP12 and the underlying mechanisms remain unclear. Thus, in this study, in an aim to shed light into this matter, the expression levels of HDAC3 and AKAP12 in 96 colorectal cancer (CRC) and adjacent non-cancerous tissues, as well as in SW480 cells were examined by immunohistochemical, RT-qPCR and western blot analyses...
February 23, 2018: International Journal of Oncology
Weiqin Jiang, Xingchen Zhang, Qinjie Chu, Sen Lu, Linfu Zhou, Xingang Lu, Chen Liu, Lingfeng Mao, Chuyu Ye, Michael P Timko, Longjiang Fan, Haixing Ju
Circular RNAs (circRNAs) have been reported that can be used as biomarkers for colorectal cancers (CRC) and other types of tumors. However, a limited number of studies have been performed investigating the potential role of circRNAs in tumor metastasis. Here, we examined the circRNAs in two CRC cell lines (a primary tumor cell SW480 and its metastasis cell SW620), and found a large set of circRNA (2,919 ncDECs) with significantly differential expression patterns relative to normal cells (NCM460). In addition, we uncovered a set of 623 pmDECs that differ between the primary CRC cells and its metastasis cells...
2018: Frontiers in Genetics
Jie Zhang, Xiao-Yan Li, Ping Hu, Yuan-Sheng Ding
Previous study indicates that long noncoding RNA NORAD could serve as a competing endogenous RNA to pancreatic cancer metastasis. However, its role in colorectal cancer requires to be investigated. In the present study, we found that the expression of NORAD was significantly upregulated in colorectal cancer (CRC) tissues. Furthermore, the expression of NORAD was positively related with CRC metastasis and patients' poor prognosis. Knockdown of NORAD markedly inhibited CRC cell proliferation, migration and invasion but induced cell apoptosis in vitro...
February 22, 2018: Oncology Research
Ji Kui Peng, Shi Qiang Shen, Ju Wang, Hong Wei Jiang, Yong Qiang Wang
Hypoxia is a common characteristic of solid tumors. Previous studies have reported that the tumor invasion-associated factor, AMPK-related protein kinase 5 (ARK5), is associated with a poor prognosis in colon cancer. However, whether or not ARK5 is involved in hypoxia is unclear. The aim of present study was to investigate the association between the expression of ARK5 and that of hypoxia-inducible factor 1-α (HIF1-α). Samples from 60 patients with colon cancer were collected and immunohistochemistry was used to detect the expression of ARK5 and HIF1-α within them...
March 2018: Oncology Letters
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