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Elvis T Ajuh, Zongsong Wu, Emma Kraus, Fabian H Weissbach, Tobias Bethge, Rainer Gosert, Nicole Fischer, Hans H Hirsch
Human polyomavirus (HPyV) DNA genomes contain three regions denoted early viral gene region (EVGR) encoding the regulatory T-antigens and one microRNA, late viral gene region (LVGR) encoding the structural Vp capsid proteins, and non-coding control region (NCCR). The NCCR harbours the origin of viral genome replication and bi-directional promoter/enhancer functions governing EVGR- and LVGR-expression on opposite DNA strands. Despite principle similarities, HPyV-NCCRs differ in length, sequence, and architecture...
January 17, 2018: Journal of Virology
F C Koyama, C M Lopes Ramos, F Ledesma, V A F Alves, J M Fernandes, B B Vailati, G P São Julião, A Habr-Gama, J Gama-Rodrigues, R O Perez, A A Camargo
BACKGROUND: Neoadjuvant chemoradiotherapy (CRT) is one of the preferred initial treatment strategies for locally advanced rectal cancer. Responses are variable, and most patients still require surgery. The aim of this study was to identify molecular mechanisms determining poor response to CRT. METHODS: Global gene expression and pathway enrichment were assessed in pretreatment biopsies from patients with non-metastatic cT2-4 N0-2 rectal cancer within 7 cm of the anal verge...
January 2018: British Journal of Surgery
Juanli Yang, Tienian Zhu, Ruijing Zhao, Dongmei Gao, Yujie Cui, Kun Wang, Yanli Guo
BACKGROUND Although downregulation of caveolin-1 (Cav-1), which is a key constituent of membrane caveolae and a regulator of cellular processes, is associated with colorectal cancer (CRC), its involvement in the disease progression is largely unknown. This study aimed to explore the role of Cav-1 in CRC and the associated mechanisms. MATERIAL AND METHODS Fresh tissues from patients with CRC and human CRC SW480 cells were used to evaluate Cav-1 and Ki-67 expression using immunohistochemistry and Western blotting...
January 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Dan Yang, Zhong-Quan Cheng, Liu Yang, Bo Hou, Jing Yang, Xiao-Nian Li, Cheng-Ting Zi, Fa-Wu Dong, Zheng-Hua Liu, Jun Zhou, Zhong-Tao Ding, Jiang-Miao Hu
Investigation of the 95% EtOH extract of stems of Dendrobium findlayanum afforded four new seco-dendrobines, findlayines A-D (1-4); two known dendrobines, dendrobine (5) and 2-hydroxydendrobine (6); and four new phenolic compounds, dendrofindlaphenols A-C (7, 9, and 10) and 6″-de-O-methyldendrofindlaphenol A (8). Compounds 1 and 2 are the first seco-dendrobines possessing a seven-membered lactam moiety, with 3 and 4 derived from the oxidative cleavage of the C-2-C-3 bond of dendrobine. The structures were established using spectroscopic methods and by comparison with literature data...
January 17, 2018: Journal of Natural Products
Adileh Ayati, Rezvan Esmaeili, Setareh Moghimi, Tayebeh Oghabi Bakhshaiesh, Zahra Eslami-S, Keivan Majidzadeh-A, Maliheh Safavi, Saeed Emami, Alireza Foroumadi
A series of 4-amino-5-cinnamoylthiazoles 3a-p were designed and synthesized as chalcone-like anticancer agents. The synthesized derivatives 3a-p were evaluated for their in vitro antiproliferative activities against three different human cancer cell lines including MCF-7, HepG2 and SW480. Most of compounds could significantly prevent proliferation of tested cell lines. In particular, the pyrrolidine derivative 3e namely (E)-1-(4-amino-2-(pyrrolidin-1-yl)thiazol-5-yl)-3-(2,4-dichlorophenyl)prop-2-en-1-one showed promising activity, especially against HepG2 cells (IC50 = 10...
January 6, 2018: European Journal of Medicinal Chemistry
Suyong Lin, Shaoqin Chen, Zhihua Chen, Qibao Dai, Chunlin Ke
PURPOSE: The purpose of this study was to investigate the relation between X-ray irradiation and epithelial-mesenchymal transition (EMT), as well as the potential mechanisms of X-ray-induced EMT in SW480 colorectal cancer (CRC) cells. METHODS: It is well known that EMT plays a critical role in invasive and metastatic of colorectal cancer progression. However, the possible role of X-ray irradiation on EMT in colorectal cancer is widely disputed and its potential mechanisms are unclear...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Dongfang Kong, Deyong Zhang, Xianqun Chu, Jing Wang
Nowadays 5-fluorouracil (5-FU)-based adjuvant chemotherapy is widely used for treating colon carcinoma. However, 5-FU resistance in the treatment of colon carcinoma has become more common and thereby new therapeutic strategies and new adjuvant drugs still need to be explored. Two 5-FU-resistant colon cancer cell lines, HCT116 and SW480, were used to investigate the effects of Schizandrin A (SchA), 5-FU, or their combination on cell viability and apoptosis. Besides, the role of miR-195 was studied to further clarify the specific function of SchA...
January 11, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Takehiro Shimada, Masashi Tsuruta, Hirotoshi Hasegawa, Koji Okabayashi, Kohei Shigeta, Takashi Ishida, Yusuke Asada, Hirofumi Suzumura, Kaoru Koishikawa, Shingo Akimoto, Yuko Kitagawa
Heat shock protein 27 (Hsp27) is a chaperone protein of low molecular weight that is produced in response to various stresses and has a cytoprotective function. In the present study we found that there is a strong correlation between sensitivity to 5-fluorouracil (5-FU) and the expression of Hsp27 in colorectal cancer. Apatorsen is an antisense oligonucleotide that targets Hsp27 and has various antitumor effects in some types of cancer, such as bladder and prostate. Although several clinical studies are currently studying apatorsen in many malignancies, to date no promising results have been reported for colorectal cancer...
January 3, 2018: Oncology Reports
Yun-Peng Sun, Li-Li Zhu, Jin-Song Liu, Yang Yu, Zhong-Yu Zhou, Gang Wang, Guo-Kai Wang
Five new limonoids, swieteliacates A-E (1-5) and a tirucallane-type triterpenoid, swietesenin (6), together with four known compounds (7-10) were isolated from fruit of Swietenia macrophylla. Their structures were determined by spectroscopic analyses. The new compounds were tested in vitro for their cytotoxic effects against five human cancer cell lines. Compound 2 exhibited moderate cytotoxic activities against SW480 and HL-60 cancer cell lines with IC50 values of 30.6 and 32.9μM, respectively.
January 8, 2018: Fitoterapia
Xin-Yue Gu, Xiao-Fei Shen, Lun Wang, Zhou-Wei Wu, Fu Li, Bin Chen, Guo-Lin Zhang, Ming-Kui Wang
The investigation of the fruits of Solanum nigrum led to the isolation of four previously undescribed steroidal alkaloids, named solanine A, 7α-OH khasianine, 7α-OH solamargine and 7α-OH solasonine, together with six known ones. The structures of the isolated compounds were elucidated unambiguously by spectroscopic data analyses and chemical methods. Solanine A represents an unusual steroidal alkaloid with an unprecedented 6/5/6/5/5/6 hexacyclic ring system, and its structure was confirmed by X-ray single crystal diffraction analysis...
January 4, 2018: Phytochemistry
Hun Min Song, Gwang Hun Park, Su Bin Park, Hyun-Seok Kim, Ho-Jun Son, Yurry Um, Jin Boo Jeong
Viticis Fructus (VF) as the dried fruit from Vitex rotundifolia L. used as a traditional medicine for treating inflammation, headache, migraine, chronic bronchitis, eye pain, and gastrointestinal infections has been reported to have antiproliferative effects against various cancer cells, including breast, lung and colorectal cancer cells. However, the molecular mechanisms by which VF mediates the inhibitory effect of the proliferation of cancer cells have not been elucidated in detail. In this study, we investigated the molecular mechanism of VF on the down-regulation of cyclin D1 and CDK4 level associated with cancer cell proliferation...
January 3, 2018: American Journal of Chinese Medicine
Kailv Sun, Sheng Wang, Jun He, Yufeng Xie, Yang He, Zhenxin Wang, Lei Qin
The nuclear receptor coactivator 5 (NCOA5) displays both coactivator and corepressor functions. Previous studies showed that alteration of NCOA5 participates in carcinogenesis and progression. However, its roles in colorectal cancer (CRC) remain unknown. Herein, we demonstrated that expression of NCOA5 in human CRC tissues was notably higher than that in adjacent tissues, which significantly correlated with clinicopathological features such as length of tumor, regional lymph node staging and cancer staging...
December 8, 2017: Oncotarget
Zhang-Gui Ding, Jian-Hai Ding, Jiang-Yuan Zhao, Wei-Xun Chunyu, Ming-Gang Li, Shao-Jie Gu, Fei Wang, Meng-Liang Wen
A new phenylspirodrimane dimer, named stachartarin A (1), was isolated from cultures of the tin mine tailings-associated fungus Stachybotrys chartarum. Its structures were elucidated by means of spectroscopic methods. At the same time, the compound was tested for its cytotoxicity against HL-60, SMMC-7721, A-549, MCF-7, and SW480 cells.
December 26, 2017: Fitoterapia
Wangdi Liao, Guohua Li, Yu You, Hongping Wan, Qiong Wu, Chongwen Wang, Nonghua Lv
This study investigated the roles of Notch‑1 in colorectal carcinoma, to assess the mechanisms. The expression of Notch‑1 and its ligand-Jagged1 was detected in human colorectal carcinoma, colorectal adenoma, paracancerous tissue and normal colorectal tissue by immunohistochemistry. Colorectal carcinoma cell lines were utilized to confirm the expression of Notch‑1 in colorectal carcinoma cells. Lentiviral- encoding Notch‑1‑siRNA, as well as Notch‑1 inhibitor was employed to silence Notch‑1 expression and to inhibit Notch‑1 activity in HT29 cells, respectively...
December 29, 2017: Oncology Reports
Yunpeng Luan, Yanmei Li, Lina Zhu, Shuangqing Zheng, Dechang Mao, Zhuxue Chen, Yong Cao
Despite its favorable clinical efficacy, oxaliplatin‑based chemotherapy frequently results in treatment withdrawal and induces liver damage in colon cancer. Therefore, it is important to develop novel drugs, which can safely and effectively complement or replace the therapeutic effects of oxaliplatin. Codonopis bulleynana Forest ex Diels (cbFeD) has wide range of pharmacological effects, including anticancer effects. In the present study, the anticancer activity of cbFeD and its potential molecular mechanisms were investigated...
December 21, 2017: International Journal of Molecular Medicine
Wan-Qi Lv, Hai-Cheng Wang, Jing Peng, Yi-Xiang Wang, Jiu-Hui Jiang, Cui-Ying Li
Background: The low efficiency of clustered, regularly interspaced, palindromic repeats-associated Cas (CRISPR/Cas) system editing genes in vivo limits the application. A components of the extracellular matrix (ECM), the extra domain A positive fibronectin (EDA+FN), may be a target for CRISPR/Cas system for the pro-oncogenic effects. The exclusion of EDA exon would alter the microenvironment and inhibit tumor progression, even the frequency of gene editing is still limited. Results: The pro-oncogenic effects were confirmed by the exclusion of EDA exon from the fibronectin gene, as illustrated by the down-regulated proliferation, migration and invasion of CNE-2Z or SW480 cells (P<0...
December 1, 2017: Oncotarget
Thomas Sithara, B P Dhanya, Arun K B, Suresh Sini, Dan Mathew, Radhakrishnan Kokkuvayil Vasu, P Nisha
Zerumbone isolated from the rhizomes of Zingiber zerumbet was for the mechanisms by which it exhibits antiproliferative activity in colorectal cancer cells (SW480). The results indicated that the zerumbone suppressed cell growth and enhanced cell apoptosis. Exposure to zerumbone induced generation of reactive oxygen species, reduced the cellular antioxidant status, decreased mitochondrial membrane potential and activated caspase 3, caspase 8 and caspase 9 (p < 0.001). It was also found that there was a decrease in the expression of Bcl 2 and elevation of Bax (p <0...
December 25, 2017: Journal of Agricultural and Food Chemistry
Xiao-Lei Huo, Zhen Pei, Yong-Zhi Li, Ling-Na Han, Xiang-Yang Tian, Yi-Qiang Zhang
OBJECTIVE: To determine the expression level of long non-coding RNA (lncRNA) imprinted maternallyexpressed transcript (H19) in colorectal cancer tissues and its effect on proliferation of colorectal cancer SW620 cells. METHODS: Real-time quantitative PCR (qRT-PCR) was applied to detect the expression of H19 in 20 paired tumor tissues and adjacent normal tissues,and in normal NCM460 cells and colorectal cancer SW480,HCT116 and SW620 cells. The specific small interfering RNA for H19 (si-H19 group) or negative control sequence (si-NC group) were transfected into SW620 cells...
November 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
Zhi-Hua Jiang, Yan-Ping Liu, Miao He, Jin-Hua Zhao, Ting-Ting Wang, Xing-Yang Feng, Hao Yue, Ren-Bo An, Yan-Hui Fu
A new abietane diterpenoid, tripterregeline A (1), together with six known diterpenoids (2-7), were isolated from the roots of Tripterygium regelii. The structure of 1 was elucidated by extensive spectroscopic methods and the known compounds were identified by comparison with data reported in the literature. All isolated compounds were evaluated for their cytotoxicities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro. Compounds 1-7 showed significant inhibitory effects against various human cancer cell lines with IC50 values ranging from 0...
December 19, 2017: Natural Product Research
Xueqin Pang, Rui Li, Dongtao Shi, Xudong Pan, Chen Ma, Guangbo Zhang, Chuanyong Mu, Weichang Chen
Colon cancer is one of the most common malignant tumors in the human body, ranking second as a gastrointestinal tumor. It has a high incidence in Europe, America and China and more than 1 million new cases of colon cancer are reported worldwide each year. The incidence of colon cancer in China has increased from 12/0.1 million in the early 1970s to 56/0.1 million at present with an annual growth rate of 4.2%, which far exceeds the international level (2%). Rhotekin (RTKN) 2, a Rho-guanosine triphosphatase (GTPase) effector, has been reported to be anti-apoptotic...
December 2017: Oncology Letters
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