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Yan-Hui Fu, Yan-Lei Ma, Gui Yan, Qing Wen, Shi Hu, Zhi-Hua Jiang, Jin-Ying Tang, Yan-Ping Liu
A phytochemical investigation on the stems and leaves of Clausena emarginata led to the isolation of a previously undescribed carbazole alkaloid, clausemargine A (1), together with 11 known analogues (2-12). The structure of 1 was elucidated by extensive spectroscopic methods and the known compounds were identified by comparisons with data reported in the literature. All known compounds (2-12) were isolated from C. emarginata for the first time. All isolated compounds were evaluated for their their antiproliferative activities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro...
May 17, 2018: Natural Product Research
Wen-Fang Yi, Duo-Zhi Chen, Xiao Ding, Xiao-Nian Li, Shun-Lin Li, Ying-Tong Di, Yu Zhang, Xiao-Jiang Hao
Four new indole alkaloids, melodinusines A-D (1, 2, 6, and 7), along with 26 known indole alkaloids, were isolated from Melodinus tenuicaudatus and Melodinus khasianus (Melodinus genus). Among them, 1 and 2 are aspidospermine-aspidospermine-type bisindole alkaloids while 7 is a novel melodinus-type alkaloid. Their structures were established on the basis of comprehensive spectroscopic data analysis and the structure of 7 was further confirmed by single-crystal X-ray diffraction analysis. Their cytotoxic activities against five human cancer cell lines, HL-60, SMMC-7721, A-549, MCF-7, and SW480 were also evaluated...
May 14, 2018: Fitoterapia
R Huang, J-Y Lin, Y-J Chi
OBJECTIVE: To investigate the effects of miR-519d on the 5-fluorouracil resistance in colorectal cancer cells and to explore the mechanism. MATERIALS AND METHODS: Colorectal cancer cells HCT116 and SW480 were transfected with miR519d-mimic or siCCND1 by transient transfection. The sensitivity of cells to 5-Fu was assayed by MTT assay. Dual luciferase assay was used to examine the effect of CCND1 on the sensitivity of cells to 5-Fu mediated by miR-519d. RESULTS: MiR-519d was overexpressed in colorectal cancer cells after transient transfection with miR-519d mimics...
May 2018: European Review for Medical and Pharmacological Sciences
Sheng-Yan Qian, Cai-Ling Yang, Afsar Khan, Rong-Xiang Chen, Ming-Song Wu, Li Tuo, Qian Wang, Jian-Guo Liu, Gui-Guang Cheng
Two new pyrazinoquinazoline alkaloids, epi-fiscalin D (1) and epi-fiscalin E (2), as well as three known analogues, norquinadoline A (3), quinadoline A (4), and fiscalin C (5), were isolated from ethyl acetate extract of the fermentation broth of Stentrophomonas maltophilia QB-77. The structures of new compounds were elucidated on the basis of extensive spectroscopic data analysis including UV, HRESIMS, and 1D and 2D NMR experiments. All the isolated compounds were tested for their in vitro cytotoxicity against five human cancer cell lines (SMMC-7721, MCF-7, HL-60, SW480, and A-549) and antibacterial activities against Bacillus subtilis, Escherichia coli, and Staphylococcus aureus...
May 17, 2018: Natural Product Research
Nandaraj Taye, Aftab Alam, Suvankar Ghorai, Deya Ghosh Chatterji, Apoorva Parulekar, Devraj Mogare, Snahlata Singh, Pallabi Sengupta, Subhrangsu Chatterjee, Manoj Kumar Bhat, Manas Kumar Santra, Prabhakar Budha Salunkhe, Susan Kling Finston, Samit Chattopadhyay
Reduced expression of Scaffold/Matrix Attachment Region Binding Protein 1 (SMAR1) is associated with various cancers resulting in poor prognosis of the diseases. However, the precise underlying mechanism elucidating the loss of SMAR1 requires ongoing study. Here, we show that SMAR1 is highly downregulated during aberrant Wnt3a signaling due to proteasomal degradation and predicted poor prognosis of colorectal cancer. However, substitution mutation (Arginine and Lysine to Alanine) in the D-box elements of SMAR1 viz...
April 20, 2018: Oncotarget
Kang He, Jinxi Wang, Juan Zou, Jichun Wu, Shaojie Huo, Jiang Du
Three new steroidal alkaloids with an unusual 3 α tigloylamide group, named sarchookloides A⁻C ( 1 ⁻ 3 ), were isolated along with four known compounds ( 4 ⁻ 7 ) from the roots of Sarcococca hookeriana . Their structures and relative configuration were elucidated on the basis of spectroscopic methods including MS, UV, IR, 1D, and 2D NMR data. The isolated compounds were evaluated for their cytotoxicity against five human cancer cell lines: Hela, A549, MCF-7, SW480, and CEM in vitro. All three amide substituted steroidal alkaloids exhibited significant cytotoxic activities with IC50 values of 1...
May 15, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Rui Zhang, Jian Xu, Jian Zhao, Jing H Bai
Recent research has shown that TOP2A plays an important role in the tumorigenesis of many malignancies, such as breast cancer, ovarian cancer, and prostate cancer. However, few studies have been conducted on TOP2A expression and functions in colon cancer. In the present study, we found that TOP2A expression was obviously elevated in colon cancer tissues compared to adjacent non-cancerous tissues. Depletion of TOP2A in HCT116 and SW480 colon cancer cells by transfection of specific small interfering RNA significantly suppressed proliferation and inhibited invasion of cells, even induced apoptosis as indicated by both MTT assay, Annexin V/propidium iodide staining, and Transwell assay...
May 15, 2018: Journal of Cellular Biochemistry
Dayong Ding, Changfeng Li, Tiancheng Zhao, Dandan Li, Lei Yang, Bin Zhang
This investigation was aimed at working out the combined role of lncRNA H19, miR-29b and Wnt signaling in the development of colorectal cancer (CRC). In the aggregate, 185 CRC tissues and corresponding para-carcinoma tissues were gathered. The human CRC cell lines (i.e. HT29, HCT116, SW480 and SW620) and normal colorectal mucosa cell line (NCM460) were also purchased. Si-H19, si-NC, miR-29b-3p mimics, miR-29b-3p inhibitor, si-PGRN and negative control (NC) were, respectively, transfected into the CRC cells...
May 10, 2018: Molecules and Cells
Chen Li, Xiaohui Du, Shaoyou Xia, Lin Chen
In the present study, the function of miR-150 and its downstream target iASPP in the growth and metastasis of colorectal cancer (CRC) cells was investigated. The expression of miR-150 and iASPP was first investigated in clinical CRC samples. Subsequently, the effects of miR-150 overexpression and iASPP inhibition on cell viability, cell cycle distribution, apoptosis, migration and invasion were detected with CCK-8, flow cytometry, scratch and Transwell assays. The interaction between miR-150 and iASPP was confirmed using a dual-luciferase assay...
April 30, 2018: Oncology Reports
Chongyao Bi, Baofei Jiang
The morbidity of colorectal cancer (CRC) increases annualy, which accounts to higher mortality worldwide. Therefore, it is important to study the pathogenesis of colon cancer. Ribophorin II (RPN2), part of the N-oligosaccharyltransferase complex, is highly expressed in CRC. In the present study, we investigated whether RPN2 can regulate apoptosis, migration and invasion by RNA interference in CRC and sought to clarify the molecular mechanism involved. Based on previous research, an abnormal high expression of RPN2 was observed in CRC tissues and cell lines by real-time (RT)-PCR, immunohistochemistry (IHC) and western blot analysis...
May 10, 2018: Oncology Reports
Han Zhang, Hong-Tao Zhu, Dong Wang, Chong-Ren Yang, Ying-Jun Zhang
Two new sphingofungins G (1) and H (2) with a five-membered lactone ring were identified from the solid-state fermentation of Aspergillus penicilliodes Speg., a dominant microorganism isolated previously from the post-fermentation of ripe Pu-er tea, together with six known metabolites. Their structures were elucidated by extensive spectroscopic methods. All of them were isolated from the mycelia culture of A. penicilliodes for the first time. The known compound, trypacidin, exhibited potent cytotoxities against five human cancer cell lines, e...
May 10, 2018: Natural Product Research
Wu Sun, Xiaojun Wang, Jialu Li, Chaoying You, Pan Lu, Huijin Feng, Yan Kong, Haiyang Zhang, Yanqing Liu, Ruihua Jiao, Xi Chen, Yi Ba
Colorectal cancer (CRC) is a very common metastatic tumor with active angiogenesis that requires active angiogenesis. Recently, increased microRNA-181a-5p (miR-181a) expression was found to be significantly associated with liver metastasis and poor outcome in CRC patients. In this study, the role of miR-181a in tumor angiogenesis was further investigated. Capillary tube formation assays were used to demonstrate the ability of miR-181a to promote tumor angiogenesis. Bioinformatics analyses identified SRC kinase signaling inhibitor 1 (SRCIN1) as a potential target of miR-181a...
April 19, 2018: Cell Death & Disease
Haiyi Feng, Ming Xu, Yunpeng Zhang, Bo Han, Jue Wang, Peng Sun
BACKGROUND: The ability to diagnose or treat colorectal cancer (CRC) at an early stage could lead to the improved survival. The purpose of this study was to identify differentially expressed microRNAs (miRNAs) involved in the pathogenesis of CRC. These miRNAs may serve as the biomarkers or potential therapeutic targets for CRC early detection or treatment. METHODS: The GSE39814 miRNA expression profile dataset was downloaded from the NCBI GEO database. MiRNAs were extracted from the exosome fractions of three normal fetal colon-derived cells and HCT116 and SW480 cell lines...
May 1, 2018: Clinical Laboratory
Yan-Ping Liu, Shi Hu, Qing Wen, Yan-Lei Ma, Zhi-Hua Jiang, Jin-Ying Tang, Yan-Hui Fu
Two novel γ-lactone derivatives, trigoheterophines A (1) and B (2), together with four known furan derivatives (3-6), were isolated from the stems and leaves of Trigonostemon heterophyllus. The structures of 1 and 2 were elucidated by extensive spectroscopic methods and the known compounds were identified by comparing with the data reported in literature. Among them, trigoheterophines A (1) and B (2) represent an unusual type of γ-lactone derivatives, possessing 21 carbon atoms on the carbon skeleton, and known compouds (3-6) are rare furan derivatives in the plant kingdom with diverse long-chain hydrocarbyl groups as substituents at C-4...
April 30, 2018: Bioorganic Chemistry
Zhao-Quan Wang, Xing-Yu Li, Dong-Bao Hu, Chun-Lin Long
Seven undescribed lavandulyl benzophenones garcimultiflorones K-Q, and fourteen known compounds were isolated from the CHCl3 soluble fraction of 95% EtOH extract of Garcinia multiflora branches. Their structures and absolute configurations were determined by spectroscopic techniques including NMR spectroscopy, MS analysis, and ECD calculations. Seven isolated compounds expect for garcimultiflorone L and garcimultiflorone O exhibited cytotoxic activities in vitro against five cancer cell lines (HL-60, A549, SMMC-7721, MCF-7, and SW480)...
May 5, 2018: Phytochemistry
Hong Peng, Qiang Su, Zhong-Chao Lin, Xiu-Hua Zhu, Ming-Sha Peng, Zhen-Bing Lv
Chitinase-3-like-1 protein (YKL-40), a member of the mammalian chitinase-like glycoproteins, serves a key role in the pathogenesis of rectal cancer. The present study examined the antitumor effect of theophylline, a pan-chitinase inhibitor, in rectal cancer in vitro and investigated the mechanism by which it acted. SW480 cell lines were treated with varying theophylline concentrations (10-2 , 10-3 , 10-4 and 10-5 mol/l). An MTT assay was used to observe cell proliferation and identify the optimal theophylline concentration...
May 2018: Oncology Letters
Hongpeng Zhao, Bo Liu, Jie Li
LIM and SH3 protein 1 (Lasp-1), a focal adhesion protein, serves a critical role in the regulation of cell proliferation and migration. The role of Lasp-1, as well as the intracellular signaling pathways involved in metastasis and progression of colorectal carcinoma, remains unclear. In the present study, the regulatory effect of Lasp-1 and the underlying molecular mechanism involved in migration and invasion of colorectal carcinoma were investigated. RNA interference and overexpression in SW480 cells were performed to elucidate the role of Lasp-1...
May 2018: Oncology Letters
Tao Li, Lei Zhang, Xinkai Huo
The inhibitory effect of aesculetin on the growth of colon cancer cell line SW480 through the Wnt/β-catenin signaling pathway was studied. The appropriate concentration of aesculetin was selected by cell counting kit-8 (CCK-8) assay, and the effect of aesculetin on the proliferation of SW480 cells was investigated by bromodeoxyuridine (BrdU) assay. The expression level of the messenger ribonucleic acid (mRNA) in β-catenin and Wnt signaling pathway target genes, c-Myc and cyclin D1 , was detected by reverse transcription-polymerase chain reaction (RT-PCR)...
May 2018: Oncology Letters
Yuanfei Li, Guoqiang Zhu, Huihong Zhai, Junmei Jia, Wenhui Yang, Xiaoqing Li, Lixin Liu
Epithelial-mesenchymal transition (EMT) is critical in the progression of numerous types of carcinoma, and endows invasive and metastatic properties upon cancer cells. The tumor microenvironment facilitates tumor metastasis to distant organs. Various signaling pathways contribute to this process. In the present study, SW480 colon adenocarcinoma cells were treated with transforming growth factor-β1 (TGF-β1; 10 ng/ml) and tumor necrosis factor-α (TNF-α; 20 ng/ml), alone or in combination, for 72 h, and EMT was assessed using immunofluorescence, western blot analysis and migration assays...
May 2018: Oncology Letters
Wenjing Wang, Xiao Wan, Junjun Liu, Jianping Wang, Hucheng Zhu, Chunmei Chen, Yonghui Zhang
A new sesquiterpenoid 9,10-diolhinokiic acid ( 1 ) and a new diterpenoid roussoellol C ( 2 ), together with 4 known compounds, were isolated from the extracts of laboratory cultures of marine-derived fungus Talaromyces purpurogenus . 9,10-diolhinokiic acid is the first thujopsene-type sesquiterpenoid containing a 9,10-diol moiety, and roussoellol C possesses a novel tetracyclic fusicoccane framework with an unexpected hydroxyl at C-4. These new structures were confirmed by spectroscopic data, chemical method, NMR data calculations and electronic circular dichroism (ECD) calculations...
May 2, 2018: Marine Drugs
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