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Fernanda S Nascimento, Yuping Wei-Pridgeon, Michael J Arrowood, Delynn Moss, Alexandre J da Silva, Eldin Talundzic, Yvonne Qvarnstrom
Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range.
November 2016: Journal of Microbiological Methods
Yaqiong Guo, Dawn M Roellig, Na Li, Kevin Tang, Michael Frace, Ynes Ortega, Michael J Arrowood, Yaoyu Feng, Yvonne Qvarnstrom, Lin Wang, Delynn M Moss, Longxian Zhang, Lihua Xiao
Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci. This new tool could be useful for case linkage and infection/contamination source tracking.
August 2016: Emerging Infectious Diseases
Mahmoud Agholi, Shahrbanou Naderi Shahabadi, Mohammad Hossein Motazedian, Gholam Reza Hatam
The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran...
June 2016: Korean Journal of Parasitology
Morgan E Milton, Scott W Nelson
Members of the phylum Apicomplexa are responsible for many devastating diseases including malaria (Plasmodium spp.), toxoplasmosis (Toxoplasma gondii), babesiosis (Babesia bovis), and cyclosporiasis (Cyclospora cayetanensis). Most Apicomplexans contain a unique and essential organelle called the apicoplast. Derived from an ancient chloroplast, the apicoplast replicates and maintains a 35 kilobase (kb) circular genome. Due to its essential nature within the parasite, drugs targeted to proteins involved in DNA replication and repair of the apicoplast should be potent and specific...
August 2016: Molecular and Biochemical Parasitology
Judit Plutzer, Panagiotis Karanis
Outbreak incidents raise the question of whether the less frequent aetiological agents of outbreaks are really less frequent in water. Alternatively, waterborne transmission could be relevant, but the lack of attention and rapid, sensitive methods to recover and detect the exogenous stages in water may keep them under-recognized. High quality information on the prevalence and detection of less frequent waterborne protozoa, such as Cyclospora cayetanensis, Toxoplasma gondii, Isospora belli, Balantidium coli, Blastocystis hominis, Entamoeba histolytica and other free-living amoebae (FLA), are not available...
September 15, 2016: Water Research
Shiyou Liu, Lin Wang, Huajun Zheng, Zhixiao Xu, Dawn M Roellig, Na Li, Michael A Frace, Kevin Tang, Michael J Arrowood, Delynn M Moss, Longxian Zhang, Yaoyu Feng, Lihua Xiao
BACKGROUND: Cyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. The investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. In this study we sequenced the genome of C. cayetanensis and inferred its metabolism and invasion components based on comparative genomic analysis. RESULTS: The genome organization, metabolic capabilities and potential invasion mechanism of C...
2016: BMC Genomics
M Mellado-Ferreiro, V Jarne-Betrán, M Arteaga-Mazuelas, M L Abínzano-Guillén
Chronic diarrhea caused by infection in immunocompetent patients is an infrequent condition in developed countries, although certain pathogens,generally parasites (Giardia lamblia, Isospora belli,Cryptosporidium, Cyclospora, Strongyloides, Ameba,Trichuris and Schistosoma) and some bacteria (Aeromonas,Plesiomonas, Campylobacter, Clostridium difficile, Salmonella or Mycobacterium tuberculosis)can cause persistent diarrhea.We present the case of a patient who showed Salmonella typhimurium in his stool culture and recovered following treatment with levofloxacin for 7 days...
2016: Anales del Sistema Sanitario de Navarra
Maysa A I Awadallah, Lobna M A Salem
AIM: This work aimed to study the role played by dogs in transmitting zoonotic enteric parasites to humans in Egypt and to analyze the risk factors associated with the occurrence of such infection in dogs. Serodiagnosis of anti-Toxocara immunoglobulin G (IgG) antibodies among human beings as well as analyzing risk factors predispose to Toxocara canis infection in human beings are another objectives of this study. MATERIALS AND METHODS: From June to December 2013, a total of 130 fecal samples from 4 dog populations (Military, nomadic and domiciled dogs from rural and high standard districts) and 150 stool samples of 6 occupational groups were examined for the presence of enteric parasitic infection...
August 2015: Veterinary World
Eman M Hussein, Amal A El-Moamly, Moushira A Mahmoud, Nayera S Ateek
A variable clinical picture of cyclosporiasis including gastrointestinal tract (GIT) symptomatic or asymptomatic beside extraintestinal consequences suggests a possibility of heterogenicity of Cyclospora cayetanensis. The present work aimed to explore the possibility of genetic variation of C. cayetanensis using high-resolution melting (HRM) curve of polymerase chain reaction (PCR) amplified 18S rRNA genes. DNAs extracted from the stool samples of 70 cyclosporiasis patients were amplified and scanned by PCR/HRM curve...
July 2016: Parasitology Research
Dinesh Bhandari, Sarmila Tandukar, Hiramani Parajuli, Pratigya Thapa, Prakash Chaudhary, Dhiraj Shrestha, Pradeep K Shah, Jatan B Sherchan, Jeevan B Sherchand
BACKGROUND: The intestinal coccidian protozoa Cyclospora cayetanensis has emerged as an important cause of parasitic diarrhea among children living in developing countries. This study aimed to determine the prevalence of Cyclospora among the school children of Kathmandu with reference to various associated risk factors. METHODOLOGY: A total of five hundred and seven stool samples from students between the age of 3-14 years, studying in 13 different schools in Kathmandu were collected during the study period (May-November, 2014) and processed at the Public Health Research Laboratory, Institute of Medicine, Kathmandu, Nepal...
December 2015: Tropical Medicine and Health
Rina Girard Kaminsky, Javier Lagos, Gabriela Raudales Santos, Samuel Urrutia
BACKGROUND: Document seasonality occurrence and epidemiologic characteristics of Cyclospora cayetanensis infections during a 10-year period from patients consulting at the University Hospital, Honduras. METHODS: Retrospective non interventional hospital-based study analyzed laboratory results from the period 2002 to 2011 of fresh and Ziehl-Nielsen carbolfuchsin stained routine stool samples received for parasitologic examination. Sporadically a sample with numerous oocysts was allowed to sporulate in 2...
2016: BMC Infectious Diseases
U Ghoshal, A Dey, P Ranjan, S Khanduja, V Agarwal, U C Ghoshal
PURPOSE: Enteric parasitic infestation is a major public health problem in developing countries. Parasites such as Cryptosporidium spp., Cyclospora spp., Cystoisospora spp. and Microsporidia may cause severe diarrhoea among immunocompromised patients. There is scanty data on their frequency among immunocompetent patients. Accordingly, we studied the frequency of enteric opportunistic parasites among immunocompetent patients with diarrhoea from northern India; we also performed genetic characterisation of Cryptosporidia and Microsporidia among them...
January 2016: Indian Journal of Medical Microbiology
Hui-Lan Wu
Wenyonella philiplevinei is one of the pathogens causing coccidiasis in ducks. The prevalence of W. philiplevinei infection in Linwu ducks (a Chinese local domesticated duck) was investigated in Linwu county (an area of Hunan province) of Hunan province, subtropical China from March 2014 to February 2015. Two hundred fifty-eight of 1800 (14.3 %) Linwu ducks were found to be infected with W. philiplevinei. To identify the species identity of the collected samples, a portion of the 18S rDNA was amplified from W...
March 2016: Tropical Animal Health and Production
Brisa Maria Fregonesi, Meire Nikaido Suzuki, Carolina Sampaio Machado, Karina Aparecida de Abreu Tonani, Ana Paula Morais Fernandes, Aline Aparecida Monroe, Maria Célia Cervi, Susana Segura-Muñoz
INTRODUCTION: Emergent and re-emergent waterborne protozoans have become a worldwide public health problem, especially among vulnerable groups. METHODS: This cross-sectional study evaluated 17 HIV-infected children and their families. RESULTS: A high (76.5%) percentage of parasite-infected children was observed, even among children with CD4+ T-cell counts of >200 cells/mm3. Giardia spp., Cryptosporidium spp. and Cyclospora spp. were observed in 41...
November 2015: Revista da Sociedade Brasileira de Medicina Tropical
Safaa M Eassa, Ekram W Abd El-Wahab, Sameh E Lotfi, Sanaa A El Masry, Hanan Z Shatat, Amira M Kotkat
Solid-waste management is associated with several health hazards, particularly parasitic infection. The objective of the study was to determine the association between risk factors and the occurrence of intestinal parasitic infections (potentially pathogenic) among municipal waste collectors in Alexandria, Egypt. A cross-sectional survey was conducted in the main municipality company in Alexandria. A total of 346 municipality solid-waste workers (MSWWs) was interviewed using an in-depth questionnaire. The type of parasitic infections among waste handlers was determined using formol-ether concentration and modified Ziehl-Neelsen technique...
April 2016: Journal of Parasitology
Yvonne Qvarnstrom, Yuping Wei-Pridgeon, Wen Li, Fernanda S Nascimento, Henry S Bishop, Barbara L Herwaldt, Delynn M Moss, Vishal Nayak, Ganesh Srinivasamoorthy, Mili Sheth, Michael J Arrowood
The parasite Cyclospora cayetanensis causes foodborne diarrheal illness. Here, we report draft genome sequences obtained from C. cayetanensis oocysts purified from a human stool sample. The genome assembly consists of 865 contigs with a total length of 44,563,857 bases. These sequences can facilitate the development of subtyping tools to aid outbreak investigations.
2015: Genome Announcements
Gordon L Nichols, Joanne Freedman, Kevin G Pollock, Caroline Rumble, Rachel M Chalmers, Peter Chiodini, Gillian Hawkins, Claire L Alexander, Gauri Godbole, Christopher Williams, Hilary A Kirkbride, Meghan Hamel, Jeremy I Hawker
Cyclospora cayetanensis was identified in 176 returned travellers from the Riviera Maya region of Mexico between 1 June and 22 September 2015; 79 in the United Kingdom (UK) and 97 in Canada. UK cases completed a food exposure questionnaire. This increase in reported Cyclospora cases highlights risks of gastrointestinal infections through travelling, limitations in Cyclospora surveillance and the need for improved hygiene in the production of food consumed in holiday resorts.
2015: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Yousry A Hawash, Laila Sh Dorgham, El-Amir M Amir, Osama F Sharaf
It has been hypothesized that chronic renal failure (CRF) predisposes patients to infection with intestinal protozoa. We tested this hypothesis with a matched case-control study to determine the prevalence of these protozoa and their diarrhea associated symptoms among 50 patients with CRF (cases) from Taif, western Saudi Arabia. Fifty diarrheal patients without CRF were recruited in the study as controls. Participants were interviewed by a structured questionnaire and stool samples were collected. Samples were thoroughly examined with microscopy and three coproantigens detection kits...
2015: Journal of Tropical Medicine
C W Hedberg, M T Osterholm
No abstract text is available yet for this article.
July 2016: Epidemiology and Infection
Ulku Karaman, Nilgun Daldal, Ali Ozer, Ozgur Enginyurt, Omer Erturk
BACKGROUND: Cyclospora species are rare among other Coccidia parasites and can cause recurrent gastroenteritis. Cyclospora spp. can infect reptiles, insects, rodents, and mammals. OBJECTIVES: The present study aimed to determine the epidemiology of Cyclospora spp. in Malatya province and its neighboring provinces. PATIENTS AND METHODS: Totally, 2281 stool samples taken from patients with digestive system complaints who referred to the polyclinics affiliated with Inonu University, Faculty of Medicine in Malatya Province and its neighboring provinces, in 2006, and whose stool specimens were submitted to the parasitology department were examined...
July 2015: Jundishapur Journal of Microbiology
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