Danielle Baptista, Zeinab Tahmasebi Birgani, Helene Widowski, Fiona Passanha, Vasili Stylianidis, Kèvin Knoops, Eva Gubbins, Cinta Iriondo, Kari-Pekka Skarp, Robbert J Rottier, Tim G Wolfs, Clemens van Blitterswijk, Vanessa LaPointe, Pamela Habibović, Niki L Reynaert, Stefan Giselbrecht, Roman Truckenmüller
The culture of lung organoids relies on drops of basement membrane matrices. This comes with limitations, for example, concerning the microscopic monitoring and imaging of the organoids in the drops. Also, the culture technique is not easily compatible with micromanipulations of the organoids. In this study, we investigated the feasibility of the culture of human bronchial organoids in defined x-, y- and z-positions in a polymer film-based microwell array platform. The circular microwells have thin round/U-bottoms...
April 2023: Materials today. Bio