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https://www.readbyqxmd.com/read/29689655/dengue-virus-infection-induces-chromatin-remodeling-at-locus-aael006536-in-the-midgut-of-aedes-aegypti
#1
Graciela Gleason-Rodríguez, Manuel Castillo-Méndez, Krystal Maya, José Ramos-Castañeda, Verónica Valverde-Garduño
OBJECTIVE: To identify and characterize Aedes aegypti's AAEL006536 gene proximal upstream cis-regulatory sequences activated by dengue virus infection. MATERIALS AND METHODS: A. aegypti Rockefeller strain mosquitoes were blood fed or infected with dengue virus 2. Open chromatinprofiling was then carried out in pools of midguts from each group of mosquitoes. RESULTS: The proximal upstream region does not contain open chromatin sites in the midguts of blood-fed mosquitoes as detected by FAIRE-qPCR...
January 2018: Salud Pública de México
https://www.readbyqxmd.com/read/29670144/chromobacterium-spp-mediate-their-anti-plasmodium-activity-through-secretion-of-the-histone-deacetylase-inhibitor-romidepsin
#2
Raúl G Saraiva, Callie R Huitt-Roehl, Abhai Tripathi, Yi-Qiang Cheng, Jürgen Bosch, Craig A Townsend, George Dimopoulos
The Chromobacterium sp. Panama bacterium has in vivo and in vitro anti-Plasmodium properties. To assess the nature of the Chromobacterium-produced anti-Plasmodium factors, chemical partition was conducted by bioassay-guided fractionation where different fractions were assayed for activity against asexual stages of P. falciparum. The isolated compounds were further partitioned by reversed-phase FPLC followed by size-exclusion chromatography; high resolution UPLC and ESI/MS data were then collected and revealed that the most active fraction contained a cyclic depsipeptide, which was identified as romidepsin...
April 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29664029/fly-ash-based-water-dispersible-powder-formulation-of-bacillus-thuringiensis-var-israelensis-development-laboratory-evaluation-against-mosquito-immatures
#3
Saravanan Tamilselvan, Arulsamy Mary Manonmani, Purushothaman Jambulingam
Background & objectives: Bacillus thuringiensis var. israelensis (Bti) formulations are presently being used for insect control. In this study, a water dispersible powder (WDP) formulation using fly ash (FA) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species. Methods: An indigenous isolate Bti (Vector Control Research Centre B17) was mass produced using a 100 l fermentor in soya-based medium...
December 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29663859/factors-contributing-to-and-strategies-to-combat-emerging-arboviruses
#4
David Michael Callender
Less than half a century ago infectious diseases appeared to be destined to be extinguished via as a culmination of medical triumphs. As focus turned towards combating non-communicable diseases, emerging and re-emerging diseases (EIDs) have bloomed from those ashes. Five epidemic mosquito-borne arboviruses (Yellow Fever virus (YFV), Dengue virus, West Nile virus, Chikungunya virus, and Zika virus) have emerged in the recent past. Arboviruses are of the utmost importance with respect to EIDs due to intensive growth of globalisation, arthropod urban fitness/adaption, and environmental changes...
April 17, 2018: Global Public Health
https://www.readbyqxmd.com/read/29657007/update-on-flea-and-tick-associated-diseases-of-cats
#5
Michael R Lappin
There are multiple vector-borne diseases in cats, the most prominent being those transmitted by mosquitos, sand flies, fleas, and ticks. Many of the agents vectored by fleas or ticks have been grown or amplified from blood or have induced antibodies in the serum of normal cats or those with clinical signs like fever and will be the focus of this manuscript. As high as 80% of fleas collected from cats contain at least one organism that could induce illness in cats or people. Anaplasma phagocytophilum, Bartonella spp...
April 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29656020/odorant-binding-protein-based-identification-of-natural-spatial-repellents-for-the-african-malaria-mosquito-anopheles-gambiae
#6
Thomas Kröber, Konstantinos Koussis, Martine Bourquin, Panagiota Tsitoura, Maria Konstantopoulou, Taiwo Sam Awolola, Francesca R Dani, Huili Qiao, Paolo Pelosi, Kostas Iatrou, Patrick M Guerin
There is increasing interest in the development of effective mosquito repellents of natural origin to reduce transmission of diseases such as malaria and yellow fever. To achieve this we have employed an in vitro competition assay involving odorant-binding proteins (OBPs) of the malaria mosquito, Anopheles gambiae, with a predominantly female expression bias to identify plant essential oils (EOs) containing bioactive compounds that target mosquito olfactory function. EOs and their fractions capable of binding to such OBPs displayed repellence against female mosquitoes in a laboratory repellence assay...
April 12, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29644025/co-breeding-association-of-aedes-albopictus-skuse-and-aedes-aegypti-linnaeus-diptera-culicidae-in-relation-to-location-and-container-size
#7
Nur Aida Hashim, Abu Hassan Ahmad, Anita Talib, Farida Athaillah, Kumara Thevan Krishnan
The occurrence of major outbreaks of dengue, and other vector borne diseases such as chikungunya and zika in tropical and subtropical regions has rendered control of the diseases a top-priority for many affected countries including Malaysia. Control of the mosquito vectors Aedes aegypti and Aedes albopictus through the reduction of breeding sites and the application of insecticides to kill immature forms and adults are the main control efforts to combat these diseases. The present study describes the association between Ae...
March 2018: Tropical Life Sciences Research
https://www.readbyqxmd.com/read/29642937/evaluation-of-a-simple-polytetrafluoroethylene-ptfe-based-membrane-for-blood-feeding-of-malaria-and-dengue-fever-vectors-in-the-laboratory
#8
Doreen J Siria, Elis P A Batista, Mercy A Opiyo, Elizangela F Melo, Robert D Sumaye, Halfan S Ngowo, Alvaro E Eiras, Fredros O Okumu
BACKGROUND: Controlled blood-feeding is essential for maintaining laboratory colonies of disease-transmitting mosquitoes and investigating pathogen transmission. We evaluated a low-cost artificial feeding (AF) method, as an alternative to direct human feeding (DHF), commonly used in mosquito laboratories. METHODS: We applied thinly-stretched pieces of polytetrafluoroethylene (PTFE) membranes cut from locally available seal tape (i.e. plumbers tape, commonly used for sealing pipe threads in gasworks or waterworks)...
April 11, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29626301/nucleoprotein-from-the-unique-human-infecting-orthobunyavirus-of-simbu-serogroup-oropouche-virus-forms-higher-order-oligomers-in-complex-with-nucleic-acids-in-vitro
#9
Juliana Londoño Murillo, Aline Diniz Cabral, Mabel Uehara, Viviam Moura da Silva, Juliete Vitorino Dos Santos, João Renato Carvalho Muniz, Leandro Farias Estrozi, Daphna Fenel, Wanius Garcia, Márcia Aparecida Sperança
Oropouche virus (OROV) is the unique known human pathogen belonging to serogroup Simbu of Orthobunyavirus genus and Bunyaviridae family. OROV is transmitted by wild mosquitoes species to sloths, rodents, monkeys and birds in sylvatic environment, and by midges (Culicoides paraensis and Culex quinquefasciatus) to man causing explosive outbreaks in urban locations. OROV infection causes dengue fever-like symptoms and in few cases, can cause clinical symptoms of aseptic meningitis. OROV contains a tripartite negative RNA genome encapsidated by the viral nucleocapsid protein (NP), which is essential for viral genome encapsidation, transcription and replication...
April 6, 2018: Amino Acids
https://www.readbyqxmd.com/read/29617987/presence-of-aedes-and-anopheles-mosquito-larvae-is-correlated-to-bacteria-found-in-domestic-water-storage-containers
#10
Louise K J Nilsson, Anil Sharma, Raj K Bhatnagar, Stefan Bertilsson, Olle Terenius
Water-storage containers are common in households where access to water is scarce, and often act as breeding sites for vector mosquitoes. Bacteria in these containers may be important for attracting or repelling ovipositing mosquitoes. We hypothesized that bacterial community composition in water-storage containers would represent either inhibitory or suitable environmental conditions for mosquito larvae. To investigate this, we characterized the bacterial community composition in water-storage containers and correlated these communities to Aedes and Anopheles larval densities...
March 29, 2018: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29617849/mosquito-saliva-the-hope-for-a-universal-arbovirus-vaccine
#11
Jessica E Manning, David M Morens, Shaden Kamhawi, Jesus G Valenzuela, Matthew Memoli
Arthropod-borne viruses (arboviruses) are taxonomically diverse causes of significant morbidity and mortality. In recent decades, important mosquito-borne viruses such as West Nile, chikungunya, dengue, and Zika have re-emerged and spread widely, in some cases pandemically, to cause serious public health emergencies. There are no licensed vaccines against most of these viruses, and vaccine development and use has been complicated by the number of different viruses to protect against, by subtype and strain variation, and by the inability to predict when and where outbreaks will occur...
March 29, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29615665/unbiased-strain-typing-of-arbovirus-directly-from-mosquitoes-using-nanopore-sequencing-a-field-forward-biosurveillance-protocol
#12
Joseph A Russell, Brittany Campos, Jennifer Stone, Erik M Blosser, Nathan Burkett-Cadena, Jonathan L Jacobs
The future of infectious disease surveillance and outbreak response is trending towards smaller hand-held solutions for point-of-need pathogen detection. Here, samples of Culex cedecei mosquitoes collected in Southern Florida, USA were tested for Venezuelan Equine Encephalitis Virus (VEEV), a previously-weaponized arthropod-borne RNA-virus capable of causing acute and fatal encephalitis in animal and human hosts. A single 20-mosquito pool tested positive for VEEV by quantitative reverse transcription polymerase chain reaction (RT-qPCR) on the Biomeme two3...
April 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29608170/multiplexed-isothermal-amplification-based-diagnostic-platform-to-detect-zika-chikungunya-and-dengue-1
#13
Ozlem Yaren, Barry W Alto, Kevin M Bradley, Patricia Moussatche, Lyudmyla Glushakova, Steven A Benner
Zika, dengue, and chikungunya viruses are transmitted by mosquitoes, causing diseases with similar patient symptoms. However, they have different downstream patient-to-patient transmission potentials, and require very different patient treatments. Thus, recent Zika outbreaks make it urgent to develop tools that rapidly discriminate these viruses in patients and trapped mosquitoes, to select the correct patient treatment, and to understand and manage their epidemiology in real time. Unfortunately, current diagnostic tests, including those receiving 2016 emergency use authorizations and fast-track status, detect viral RNA by reverse transcription polymerase chain reaction (RT-PCR), which requires instrumentation, trained users, and considerable sample preparation...
March 13, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29607356/standardization-of-in-vitro-assays-to-evaluate-the-activity-of-polyherbal-siddha-formulations-against-chikungunya-virus-infection
#14
Jaspreet Jain, Somnath Pai, Sujatha Sunil
Chikungunya, a viral fever caused by Aedes mosquito results in extreme morbidity in affected individuals and is a major public health concern in India. Currently, modern vaccines or formulations prescribed by physicians can only provide symptomatic relief for the pyretic and post pyretic phase of the disease. Siddha practitioners follows strict medical regimen based on traditional Indian knowledge/concepts to treat Chikungunya with considerable results. The current study was undertaken to standardize assays for the study of these siddha formulations and to check their efficacy and potential mode of action as antivirals for Chikungunya virus infection in in vitro system...
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29605533/a-comparative-analysis-of-corpora-allata-corpora-cardiaca-microrna-repertories-revealed-significant-changes-during-mosquito-metamorphosis
#15
Marcela Nouzova, Kayvan Etebari, Fernando G Noriega, Sassan Asgari
The corpora allata (CA) are a pair of endocrine glands with neural connections to the brain and close association with another neuroendocrine organ, the corpora cardiaca (CC). The CA from adult female Aedes aegypti mosquitoes synthesizes fluctuating levels of juvenile hormone (JH), which have been linked to the ovarian development and are influenced by nutritional signals. In this study, we investigated the potential involvement of microRNAs (miRNAs), a type of small non-coding RNAs, in the regulation of gene expression in CA-CC complexes during mosquito reproductive development, at stages with distinct JH biosynthesis patterns...
March 29, 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29590133/the-effect-of-pond-dyes-on-oviposition-and-survival-in-wild-uk-culex-mosquitoes
#16
Natali Ortiz-Perea, Rebecca Gander, Oliver Abbey, Amanda Callaghan
British Culex pipiens complex [Culex pipiens sensu lato) mosquito distribution, abundance, and potential for disease transmission are intimately linked to their environment. Pond and lake dyes that block light to restrict algal photosynthesis are a relatively new product assumed to be an environmentally friendly since they are based on food dyes. Their use in urban garden ponds raises questions linked to mosquito oviposition, since coloured water can be an attractant. Culex (mostly pipiens) is commonly found in UK gardens and is a potential vector of viruses including the West Nile Virus (WNV)...
2018: PloS One
https://www.readbyqxmd.com/read/29568248/sterile-insect-technique-and-wolbachia-symbiosis-as-potential-tools-for-the-control-of-the-invasive-species-drosophila-suzukii
#17
REVIEW
Katerina Nikolouli, Hervé Colinet, David Renault, Thomas Enriquez, Laurence Mouton, Patricia Gibert, Fabiana Sassu, Carlos Cáceres, Christian Stauffer, Rui Pereira, Kostas Bourtzis
Drosophila suzukii , a vinegar fly originated from Southeast Asia, has recently invaded western countries, and it has been recognized as an important threat of a wide variety of several commercial soft fruits. This review summarizes the current information about the biology and dispersal of D. suzukii and discusses the current status and prospects of control methods for the management of this pest. We highlight current knowledge and ongoing research on innovative environmental-friendly control methods with emphasis on the sterile insect technique (SIT) and the incompatible insect technique (IIT)...
2018: Journal of Pest Science
https://www.readbyqxmd.com/read/29566214/laboratory-and-field-evaluation-of-multiple-compound-attractants-to-culex-pipiens-pallens
#18
Jiaxin Tian, Jie Mao, Baoting Yu, Hatem Fouad, Hassan Ga'al, Guofeng Mao, Jianchu Mo
Efforts to develop mosquito attractants using vertebrate host volatiles have been well made under laboratory conditions but their attractiveness to mosquitoes in the wild still needs to be evaluated. In the present study, we evaluated the attraction of female Culex pipiens pallens Coquillett (Diptera: Culicidae) to 11 individual chemical compounds found in vertebrate host odors, and to synthetic blends, consisting of different combinations of the compounds. These tests were conducted under laboratory and field conditions using a Y-tube olfactometer and odor-baited traps, respectively...
March 16, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29540804/sexual-transmission-of-zika-virus-enhances-in-utero-transmission-in-a-mouse-model
#19
Nisha K Duggal, Erin M McDonald, Jana M Ritter, Aaron C Brault
Zika virus (ZIKV) is an emerging mosquito-borne virus that can cause ZIKV congenital syndrome when a pregnant woman is infected. Sexual transmission has also been described for ZIKV, though the relationship between sexual transmission and vertical transmission has not been investigated. Here, viral dissemination to the female reproductive tract and fetuses was assessed in immunodeficient (AG129) female mice that were exposed to ZIKV by subcutaneous (s.c.) inoculation, intravaginal (ivag.) inoculation, or sexual transmission from infected male AG129 mice...
March 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29536828/zika-virus-epidemiology-from-uganda-to-world-pandemic-an-update
#20
C Talero-Gutiérrez, A Rivera-Molina, C Pérez-Pavajeau, I Ossa-Ospina, C Santos-García, M C Rojas-Anaya, A de-la-Torre
Zika virus (ZIKV) infection is an emergent worldwide public health problem. Historically, 84 countries have reported vector-borne ZIKV transmission, 61 of which report on-going transmission. It is a Flavivirus transmitted through arthropods belonging to the Aedes genus. Since 2015, ZIKV infections have increased dramatically; with 1.3 million people infected during 2015 in Brazil alone. This paper's objective is to highlight the conjectural epidemiological points of the virus' dissemination. The digital archives Pubmed, MEDLINE, EMBASE and Cochrane were searched for papers that assessed aspects of ZIKV transmission and epidemiology...
March 14, 2018: Epidemiology and Infection
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