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https://www.readbyqxmd.com/read/28339458/rapid-surveillance-for-vector-presence-rsvp-development-of-a-novel-system-for-detecting-aedes-aegypti-and-aedes-albopictus
#1
Brian L Montgomery, Martin A Shivas, Sonja Hall-Mendelin, Jim Edwards, Nicholas A Hamilton, Cassie C Jansen, Jamie L McMahon, David Warrilow, Andrew F van den Hurk
BACKGROUND: The globally important Zika, dengue and chikungunya viruses are primarily transmitted by the invasive mosquitoes, Aedes aegypti and Aedes albopictus. In Australia, there is an increasing risk that these species may invade highly urbanized regions and trigger outbreaks. We describe the development of a Rapid Surveillance for Vector Presence (RSVP) system to expedite presence- absence surveys for both species. METHODOLOGY/PRINCIPAL FINDINGS: We developed a methodology that uses molecular assays to efficiently screen pooled ovitrap (egg trap) samples for traces of target species ribosomal RNA...
March 24, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28332400/oligonucleotide-sensor-based-on-selective-capture-of-upconversion-nanoparticles-triggered-by-target-induced-dna-inter-strand-ligand-reaction
#2
Diego Mendez-Gonzalez, Marco Laurenti, Alfonso Latorre, Álvaro Somoza, Ana Vazquez, Ana Isabel Negredo, Enrique Lopez-Cabarcos, Oscar Gómez Calderón, Sonia Melle, Jorge Rubio-Retama
We present a sensor that exploits the phenomenon of upconversion luminescence to detect the presence of specific sequences of small oligonucleotides like miRNAs among others. The sensor is based on NaYF4:Yb,Er@SiO2 nanoparticles functionalized with ssDNA that contain azide groups on the 3' ends. In the presence of a target sequence, inter-strand ligation is possible via click-reaction between one azide of the upconversion probe and a DBCO-ssDNA-biotin probe present in the solution. As result of this specific and selective process, biotin is covalently attached to the surface of the upconversion nanoparticles...
March 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28332087/boswellia-ovalifoliolata-burseraceae-essential-oil-as-an-eco-friendly-larvicide-toxicity-against-six-mosquito-vectors-of-public-health-importance-non-target-mosquito-fishes-backswimmers-and-water-bugs
#3
Giovanni Benelli, Mohan Rajeswary, Periasamy Vijayan, Sengamalai Senthilmurugan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Marimuthu Govindarajan
The use of synthetic pesticides to control vector populations is detrimental to human health and the environment and may lead to the development of resistant strains. Plants can be alternative sources of safer compounds effective on mosquito vectors. In this study, the mosquito larvicidal activity of Boswellia ovalifoliolata leaf essential oil (EO) was evaluated against Anopheles stephensi, Anopheles subpictus, Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Culex tritaeniorhynchus. GC-MS revealed that the B...
March 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28331047/ascension-island-a-survey-to-assess-the-presence-of-zika-virus-vectors
#4
G Bullivant, A F Martinou
BACKGROUND AND AIM: Following concerns regarding the spread of Zika virus, Joint Services Health Unit (Cyprus) were tasked to carry out a mosquito survey on the Ascension Island, South Atlantic. This was to determine if vectors of the virus such as Aedes aegypti and Aedes albopictus were introduced and established on the Island. METHODS: An extensive survey of residential areas and natural habitats was initiated in order to collect mosquito larvae and adults by methods such as larval sampling, adult trapping and human landing catches...
March 22, 2017: Journal of the Royal Army Medical Corps
https://www.readbyqxmd.com/read/28317911/chimeric-viruses-between-rocio-and-west-nile-the-role-for-rocio-prm-e-proteins-in-virulence-and-inhibition-of-interferon-%C3%AE-%C3%AE-signaling
#5
Alberto A Amarilla, Yin X Setoh, Parthiban Periasamy, Nias Y Peng, Gabor Pali, Luiz T Figueiredo, Alexander A Khromykh, Victor H Aquino
Mosquito-transmitted flavivirus Rocio (ROCV) was responsible for an outbreak of encephalitis in the Ribeira Valley, located in the south coast of Sao Paulo State, Brazil, in 1975-1976. ROCV also causes fatal encephalitis in adult mice. Seroprevalence studies in humans, horses and water buffaloes in different regions of Brazil have suggested that ROCV is still circulating in the country, indicating the risk of re-emergence of this virus. West Nile virus (WNV) is also a mosquito-transmitted encephalitic flavivirus, however, WNV strains circulating in Australia have not been associated with outbreaks of disease in humans and exhibit low virulence in adult mice...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316881/occurrence-of-concurrent-infections-with-multiple-serotypes-of-dengue-viruses-during-2013-2015-in-northern-kerala-india
#6
Manchala Nageswar Reddy, Ranjeet Dungdung, Lathika Valliyott, Rajendra Pilankatta
BACKGROUND: Dengue is a global human public health threat, causing severe morbidity and mortality. The occurrence of sequential infection by more than one serotype of dengue virus (DENV) is a major contributing factor for the induction of Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS), two major medical conditions caused by DENV infection. However, there is no specific drug or vaccine available against dengue infection. There are reports indicating the increased incidence of concurrent infection of dengue in several tropical and subtropical regions...
2017: PeerJ
https://www.readbyqxmd.com/read/28300212/effect-of-pesticides-on-microbial-communities-in-container-aquatic-habitats
#7
Ephantus J Muturi, Ravi Kiran Donthu, Christopher J Fields, Imelda K Moise, Chang-Hyun Kim
Container aquatic habitats support a specialized community of macroinvertebrates (e.g. mosquitoes) that feed on microbial communities associated with decaying organic matter. These aquatic habitats are often embedded within and around agricultural lands and are frequently exposed to pesticides. We used a microcosm approach to examine the single and combined effects of two herbicides (atrazine, glyphosate), and three insecticides (malathion, carbaryl, permethrin) on microbial communities of container aquatic habitats...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287375/variation-in-aedes-aegypti-mosquito-competence-for-zika-virus-transmission
#8
Christopher M Roundy, Sasha R Azar, Shannan L Rossi, Jing H Huang, Grace Leal, Ruimei Yun, Ildefonso Fernandez-Salas, Christopher J Vitek, Igor A D Paploski, Uriel Kitron, Guilherme S Ribeiro, Kathryn A Hanley, Scott C Weaver, Nikos Vasilakis
To test whether Zika virus has adapted for more efficient transmission by Aedes aegypti mosquitoes, leading to recent urban outbreaks, we fed mosquitoes from Brazil, the Dominican Republic, and the United States artificial blood meals containing 1 of 3 Zika virus strains (Senegal, Cambodia, Mexico) and monitored infection, dissemination, and virus in saliva. Contrary to our hypothesis, Cambodia and Mexica strains were less infectious than the Senegal strain. Only mosquitoes from the Dominican Republic transmitted the Cambodia and Mexica strains...
April 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28284628/japanese-encephalitis-virus-infection-diagnosis-and-control-in-domestic-animals
#9
REVIEW
Karen L Mansfield, Luis M Hernández-Triana, Ashley C Banyard, Anthony R Fooks, Nicholas Johnson
Japanese encephalitis virus (JEV) is a significant cause of neurological disease in humans throughout Asia causing an estimated 70,000 human cases each year with approximately 10,000 fatalities. The virus contains a positive sense RNA genome within a host-derived membrane and is classified within the family Flaviviridae. Like many flaviviruses, it is transmitted by mosquitoes, particularly those of the genus Culex in a natural cycle involving birds and some livestock species. Spill-over into domestic animals results in a spectrum of disease ranging from asymptomatic infection in some species to acute neurological signs in others...
March 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28284278/mutagen-resistance-and-mutation-restriction-of-st-louis-encephalitis-virus
#10
Sara B Griesemer, Laura D Kramer, Greta A Van Slyke, Janice D Pata, David W Gohara, Craig E Cameron, Alexander T Ciota
The error rate of the RNA-dependent RNA polymerase (RdRp) of RNA viruses is important in maintaining genetic diversity for viral adaptation and fitness. Numerous studies have shown that mutagen-resistant RNA virus variants display amino acid mutations in the RdRp and other replicase subunits, which in turn exhibit an altered fidelity phenotype affecting viral fitness, adaptability and pathogenicity. St. Louis encephalitis virus (SLEV), like its close relative West Nile virus, is a mosquito-borne flavivirus that has the ability to cause neuroinvasive disease in humans...
February 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28283817/a-novel-rhabdovirus-related-to-merida-virus-in-field-collected-mosquitoes-from-anatolia-and-thrace
#11
Koray Ergünay, Annika Brinkmann, Nadine Litzba, Filiz Günay, Sırrı Kar, Kerem Öter, Serra Örsten, Yasemen Sarıkaya, Bülent Alten, Andreas Nitsche, Yvonne-Marie Linton
Next-generation sequencing technologies have significantly facilitated the discovery of novel viruses, and metagenomic surveillance of arthropods has enabled exploration of the diversity of novel or known viral agents. We have identified a novel rhabdovirus that is genetically related to the recently described Merida virus via next-generation sequencing in a mosquito pool from Thrace. The complete viral genome contains 11,798 nucleotides with 83% genome-wide nucleotide sequence similarity to Merida virus. Five major putative open reading frames that follow the canonical rhabdovirus genome organization were identified...
March 10, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28279191/effectiveness-of-autodissemination-stations-containing-pyriproxyfen-in-reducing-immature-aedes-albopictus-populations
#12
Isik Unlu, Devi S Suman, Yi Wang, Kim Klingler, Ary Faraji, Randy Gaugler
BACKGROUND: Aedes albopictus, the Asian tiger mosquito, is an aggressive, highly anthropophilic, day-biting mosquito with an expanding geographic range. Suppression of Ae. albopictus is difficult because of the abundance and prevalence of larval habitats within peridomestic environments, particularly cryptic habitats such as corrugated extension spouts, fence post openings, discarded food containers, etc. Because of the challenges of eliminating or treating larval habitats of this species, we tested an autodissemination concept to contaminate these habitats with the insect growth regulator pyriproxyfen...
March 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28274805/expression-refolding-and-bio-structural-analysis-of-a-tetravalent-recombinant-dengue-envelope-domain-iii-protein-for-serological-diagnosis
#13
Maxime Combe, Xavier Lacoux, Jérôme Martinez, Odile Méjan, Françoise Luciani, Soizic Daniel
Dengue is a mosquito-borne disease caused by four genetically and serologically related viruses that affect several millions of people. Envelope domain III (EDIII) of the viral envelope protein contains dengue virus (DENV) type-specific and DENV complex-reactive antigenic sites. Here, we describe the expression in Escherichia coli, the refolding and bio-structural analysis of envelope domain III of the four dengue serotypes as a tetravalent dengue protein (EDIIIT2), generating an attractive diagnostic candidate...
March 6, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28273394/inhibition-of-type-i-interferon-induction-and-signalling-by-mosquito-borne-flaviviruses
#14
REVIEW
Stephanie L Cumberworth, Jordan J Clark, Alain Kohl, Claire L Donald
The Flavivirus genus (Flaviviridae family) contains a number of important human pathogens, including dengue and Zika viruses, which have the potential to cause severe disease. In order to efficiently establish a productive infection in mammalian cells, flaviviruses have developed key strategies to counteract host immune defences, including the type I interferon response. They employ different mechanisms to control interferon signal transduction and effector pathways and key research generated over the past couple of decades has uncovered new insights into their abilities to actively decrease interferon antiviral activity...
March 8, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28257813/reverse-osmosis-and-ultrafiltration-for-recovery-and-reuse-of-larval-rearing-water-in-anopheles-arabiensis-mass-production-effect-of-water-quality-on-larval-development-and-fitness-of-emerging-adults
#15
Wadaka Mamai, Rebecca Hood-Nowotny, Hamidou Maiga, Adel Barakat Ali, Nanwintoun S Bimbile-Somda, Diloma Dieudonné Soma, Hanano Yamada, Rosemary Susan Lees, Jeremie R L Gilles
BACKGROUND: Countries around the world are showing increased interest in applying the sterile insect technique against mosquito disease vectors. Many countries in which mosquitoes are endemic, and so where vector control using the sterile insect technique may be considered, are located in arid zones where water provision can be costly or unreliable. Water reuse provides an alternate form of water supply. In order to reduce the cost of mass rearing of Anopheles arabiensis mosquitoes, the possibility of recycling and reusing larval rearing water was explored...
March 1, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28257079/-looking-over-the-backyard-fence-householders-and-mosquito-control
#16
Samir Mainali, Ram Sharan Lamichhane, Kim Clark, Shelley Beatty, Maria Fatouros, Peter Neville, Jacques Oosthuizen
(1) Background: Vector-borne diseases are a significant public health problem in Western Australia. Mosquitoes are responsible for the transmission of a number of pathogens and may pose a serious nuisance problem. Prevention efforts in the State are multi-faceted and include physical, chemical, and cultural control methods for restricting mosquito breeding. This is less complex where breeding areas are located within public open spaces. In Australia's developed urban areas, breeding sites are, however, frequently located within private residential landholdings, where the scope of public health officials to act is constrained by law and practicality...
March 2, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28236746/discovery-of-a-novel-antiviral-agent-targeting-the-nonstructural-protein-4-nsp4-of-chikungunya-virus
#17
Yuji Wada, Yasuko Orba, Michihito Sasaki, Shintaro Kobayashi, Michael J Carr, Haruaki Nobori, Akihiko Sato, William W Hall, Hirofumi Sawa
Chikungunya fever (CHIKF) is caused by chikungunya virus (CHIKV) infection which is a re-emerging mosquito-borne zoonosis. At present, there are no approved therapeutics for CHIKF. Herein, we have investigated candidate compounds which can inhibit CHIKV infection. Screening of chemical compound libraries were performed and one candidate, a benzimidazole-related compound designated Compound-A was found to inhibit infection by several CHIKV strains and a Sindbis virus strain at nanomolar concentrations. To investigate the inhibitory mechanism of action, a Compound-A resistant CHIKV (res-CHIKV) was isolated and a key mutation associated with resistance was identified by reverse-genetic recombinant CHIKVs containing amino acid substitutions present in res-CHIKV...
February 22, 2017: Virology
https://www.readbyqxmd.com/read/28215858/molecular-survey-of-dirofilaria-immitis-and-dirofilaria-repens-by-new-real-time-taqman-%C3%A2-pcr-assay-in-dogs-and-mosquitoes-diptera-culicidae-in-corsica-france
#18
Djamel Tahir, Fadi Bittar, Hélène Barré-Cardi, Doudou Sow, Mustapha Dahmani, Oleg Mediannikov, Didier Raoult, Bernard Davoust, Philippe Parola
Dirofilaria immitis and D. repens are filarioid nematodes of animals and humans, transmitted by the bite of infected mosquitoes. Domestic and wild canids are a major natural host and reservoir for these parasites. In this study, we designed a duplex real-time PCR protocol targeting the mitochondrial cytochrome c oxidase subunit I (COI) gene, detecting both D. immitis and D. repens using two primer pairs and two Dirofilaria-specific hydrolysable probes. The sensitivity and specificity of the primers and probes were tested in both experimental and naturally infected samples...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28212636/experimental-hut-evaluation-of-a-novel-long-lasting-non-pyrethroid-durable-wall-lining-for-control-of-pyrethroid-resistant-anopheles-gambiae-and-anopheles-funestus-in-tanzania
#19
Robert Malima, Basiliana Emidi, Louisa A Messenger, Richard M Oxborough, Bernard Batengana, Wema Sudi, Sophie Weston, George Mtove, Joseph P Mugasa, Franklin W Mosha, Mark W Rowland, William Kisinza
BACKGROUND: A novel, insecticide-treated, durable wall lining (ITWL), which mimics indoor residual spraying (IRS), has been developed to provide prolonged vector control when fixed to the inner walls of houses. PermaNet(®) ITWL is a polypropylene material containing non-pyrethroids (abamectin and fenpyroximate) which migrate gradually to the surface. METHODS: An experimental hut trial was conducted in an area of pyrethroid-resistant Anopheles gambiae s.l. and Anopheles funestus s...
February 17, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28207332/leaking-containers-success-and-failure-in-controlling-the-mosquito-aedes-aegypti-in-brazil
#20
Ilana Löwy
In 1958, the Pan American Health Organization declared that Brazil had successfully eradicated the mosquito Aedes aegypti, responsible for the transmission of yellow fever, dengue fever, chikungunya, and Zika virus. Yet in 2016 the Brazilian minister of health described the situation of dengue fever as "catastrophic." Discussing the recent epidemic of Zika virus, which amplified the crisis produced by the persistence of dengue fever, Brazil's president declared in January 2016 that "we are in the process of losing the war against the mosquito Aedes aegypti...
April 2017: American Journal of Public Health
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