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https://www.readbyqxmd.com/read/28215858/molecular-survey-of-dirofilaria-immitis-and-dirofilaria-repens-by-new-real-time-taqman-%C3%A2-pcr-assay-in-dogs-and-mosquitoes-diptera-culicidae-in-corsica-france
#1
Djamel Tahir, Fadi Bittar, Hélène Barré-Cardi, Doudou Sow, Mustapha Dahmani, Oleg Mediannikov, Didier Raoult, Bernard Davoust, Philippe Parola
Dirofilaria immitis and D. repens are filarioid nematodes of animals and humans, transmitted by the bite of infected mosquitoes. Domestic and wild canids are a major natural host and reservoir for these parasites. In this study, we designed a duplex real-time PCR protocol targeting the mitochondrial cytochrome c oxidase subunit I (COI) gene, detecting both D. immitis and D. repens using two primer pairs and two Dirofilaria-specific hydrolysable probes. The sensitivity and specificity of the primers and probes were tested in both experimental and naturally infected samples...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28212636/experimental-hut-evaluation-of-a-novel-long-lasting-non-pyrethroid-durable-wall-lining-for-control-of-pyrethroid-resistant-anopheles-gambiae-and-anopheles-funestus-in-tanzania
#2
Robert Malima, Basiliana Emidi, Louisa A Messenger, Richard M Oxborough, Bernard Batengana, Wema Sudi, Sophie Weston, George Mtove, Joseph P Mugasa, Franklin W Mosha, Mark W Rowland, William Kisinza
BACKGROUND: A novel, insecticide-treated, durable wall lining (ITWL), which mimics indoor residual spraying (IRS), has been developed to provide prolonged vector control when fixed to the inner walls of houses. PermaNet(®) ITWL is a polypropylene material containing non-pyrethroids (abamectin and fenpyroximate) which migrate gradually to the surface. METHODS: An experimental hut trial was conducted in an area of pyrethroid-resistant Anopheles gambiae s.l. and Anopheles funestus s...
February 17, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28207332/leaking-containers-success-and-failure-in-controlling-the-mosquito-aedes-aegypti-in-brazil
#3
Ilana Löwy
In 1958, the Pan American Health Organization declared that Brazil had successfully eradicated the mosquito Aedes aegypti, responsible for the transmission of yellow fever, dengue fever, chikungunya, and Zika virus. Yet in 2016 the Brazilian minister of health described the situation of dengue fever as "catastrophic." Discussing the recent epidemic of Zika virus, which amplified the crisis produced by the persistence of dengue fever, Brazil's president declared in January 2016 that "we are in the process of losing the war against the mosquito Aedes aegypti...
February 16, 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/28206869/acoustic-control-of-mosquito-larvae-in-artificial-drinking-water-containers
#4
Seth C Britch, Herbert Nyberg, Robert L Aldridge, Tom Swan, Kenneth J Linthicum
Emerging technology designed to kill mosquito larvae with sound waves may present a nonchemical and nonbiological alternative to reduce larval populations of key medically important mosquito species such as Aedes aegypti in containers or catchments of water. These devices could benefit integrated vector management programs facing public resistance to the use of chemical or biological larvicides in stored drinking water. In this study we investigate the efficacy of a Larvasonic SD-Mini Acoustic Larvicide device in reducing larval populations of Ae...
December 2016: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28199846/frequent-zika-virus-sexual-transmission-and-prolonged-viral-rna-shedding-in-an-immunodeficient-mouse-model
#5
Nisha K Duggal, Jana M Ritter, Samuel E Pestorius, Sherif R Zaki, Brent S Davis, Gwong-Jen J Chang, Richard A Bowen, Aaron C Brault
Circulation of Zika virus (ZIKV) was first identified in the Western hemisphere in late 2014. Primarily transmitted through mosquito bite, ZIKV can also be transmitted through sex and from mother to fetus, and maternal ZIKV infection has been associated with fetal malformations. We assessed immunodeficient AG129 mice for their capacity to shed ZIKV in semen and to infect female mice via sexual transmission. Infectious virus was detected in semen between 7 and 21 days post-inoculation, and ZIKV RNA was detected in semen through 58 days post-inoculation...
February 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28199323/a-large-scale-biorational-approach-using-bacillus-thuringiensis-israeliensis-strain-am65-52-for-managing-aedes-aegypti-populations-to-prevent-dengue-chikungunya-and-zika-transmission
#6
Catherine A Pruszynski, Lawrence J Hribar, Robert Mickle, Andrea L Leal
BACKGROUND: Aedes aegypti is a container-inhabiting mosquito and a vector of dengue, chikungunya, and Zika viruses. In 2009 several cases of autochthonous dengue transmission were reported in Key West, Florida, USA prompting a comprehensive response to control A. aegypti. In Key West, larvae of this mosquito develop in containers around human habitations which can be numerous and labor intensive to find and treat. Aerial applications of larvicide covering large areas in a short time can be an efficient and economical method to control A...
2017: PloS One
https://www.readbyqxmd.com/read/28199305/sterile-protection-against-human-malaria-by-chemoattenuated-pfspz-vaccine
#7
Benjamin Mordmüller, Güzin Surat, Heimo Lagler, Sumana Chakravarty, Andrew S Ishizuka, Albert Lalremruata, Markus Gmeiner, Joseph J Campo, Meral Esen, Adam J Ruben, Jana Held, Carlos Lamsfus Calle, Juliana B Mengue, Tamirat Gebru, Javier Ibáñez, Mihály Sulyok, Eric R James, Peter F Billingsley, K C Natasha, Anita Manoj, Tooba Murshedkar, Anusha Gunasekera, Abraham G Eappen, Tao Li, Richard E Stafford, Minglin Li, Phil L Felgner, Robert A Seder, Thomas L Richie, B Kim Lee Sim, Stephen L Hoffman, Peter G Kremsner
A highly protective malaria vaccine would greatly facilitate the prevention and elimination of malaria and containment of drug-resistant parasites. A high level (more than 90%) of protection against malaria in humans has previously been achieved only by immunization with radiation-attenuated Plasmodium falciparum (Pf) sporozoites (PfSPZ) inoculated by mosquitoes; by intravenous injection of aseptic, purified, radiation-attenuated, cryopreserved PfSPZ ('PfSPZ Vaccine'); or by infectious PfSPZ inoculated by mosquitoes to volunteers taking chloroquine or mefloquine (chemoprophylaxis with sporozoites)...
February 15, 2017: Nature
https://www.readbyqxmd.com/read/28196962/spatial-and-temporal-analysis-of-alphavirus-replication-and-assembly-in-mammalian-and-mosquito-cells
#8
Joyce Jose, Aaron B Taylor, Richard J Kuhn
Sindbis virus (SINV [genus Alphavirus, family Togaviridae]) is an enveloped, mosquito-borne virus. Alphaviruses cause cytolytic infections in mammalian cells while establishing noncytopathic, persistent infections in mosquito cells. Mosquito vector adaptation of alphaviruses is a major factor in the transmission of epidemic strains of alphaviruses. Though extensive studies have been performed on infected mammalian cells, the morphological and structural elements of alphavirus replication and assembly remain poorly understood in mosquito cells...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28196656/a-cdna-clone-launched-platform-for-high-yield-production-of-inactivated-zika-vaccine
#9
Yujiao Yang, Chao Shan, Jing Zou, Antonio E Muruato, Diniz Nunes Bruno, Barbosa de Almeida Medeiros Daniele, Pedro F C Vasconcelos, Shannan L Rossi, Scott C Weaver, Xuping Xie, Pei-Yong Shi
A purified inactivated vaccine (PIV) using the Zika virus (ZIKV) Puerto Rico strain PRVABC59 showed efficacy in monkeys, and is currently in a phase I clinical trial. High-yield manufacture of this PIV is essential for its development and vaccine access. Here we report an infectious cDNA clone-launched platform to maximize its yield. A single NS1 protein substitution (K265E) was identified to increase ZIKV replication on Vero cells (a cell line approved for vaccine production) for both Cambodian FSS13025 and Puerto Rico PRVABC59 strains...
February 6, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28192520/holding-back-the-tiger-successful-control-program-protects-australia-from-aedes-albopictus-expansion
#10
Mutizwa Odwell Muzari, Gregor Devine, Joseph Davis, Bruce Crunkhorn, Andrew van den Hurk, Peter Whelan, Richard Russell, James Walker, Peter Horne, Gerhard Ehlers, Scott Ritchie
BACKGROUND: The Asian tiger mosquito, Aedes albopictus, is an important vector of dengue, chikungunya and Zika viruses and is a highly invasive and aggressive biter. Established populations of this species were first recognised in Australia in 2005 when they were discovered on islands in the Torres Strait, between mainland Australia and Papua New Guinea. A control program was implemented with the original goal of eliminating Ae. albopictus from the Torres Strait. We describe the evolution of management strategies that provide a template for Ae...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28174057/sweet-waste-extract-uptake-by-a-mosquito-vector-survival-biting-fecundity-responses-and-potential-epidemiological-significance
#11
Hamady Dieng, Tomomitsu Satho, Fatimah Abang, Nur Khairatun Khadijah Binti Meli, Idris A Ghani, Cirilo Nolasco-Hipolito, Hafijah Hakim, Fumio Miake, Abu Hassan Ahmad, Sabina Noor, Wan Fatma Zuharah, Hamdan Ahmad, Abdul Hafiz A Majid, Ronald E Morales Vargas, Noppawan P Morales, Siriluck Attrapadung, Gabriel Tonga Noweg
In nature, adult mosquitoes typically utilize nectar as their main energy source, but they can switch to other as yet unidentified sugary fluids. Contemporary lifestyles, with their associated unwillingness to consume leftovers and improper disposal of waste, have resulted in the disposal of huge amounts of waste into the environment. Such refuse often contains unfinished food items, many of which contain sugar and some of which can collect water from rain and generate juices. Despite evidence that mosquitoes can feed on sugar-rich suspensions, semi-liquids, and decaying fruits, which can be abundant in garbage sites, the impacts of sweet waste fluids on dengue vectors are unknown...
February 4, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28159753/requirements-for-driving-anti-pathogen-effector-genes-into-populations-of-disease-vectors-by-homing
#12
Andrea Beaghton, Andrew Hammond, Tony Nolan, Andrea Crisanti, H Charles J Godfray, Austin Burt
There is a need for new interventions against the on-going burden of vector-borne diseases such as malaria and dengue. One suggestion has been to develop genes encoding effector molecules that block parasite development within the vector, and then use the nuclease-based homing reaction as a form of gene drive to spread those genes through target populations. If the effector gene reduces the fitness of the mosquito and does not contribute to the drive, then loss-of-function mutations in the effector will eventually replace functional copies, but protection may nonetheless persist sufficiently long to provide a public health benefit...
February 3, 2017: Genetics
https://www.readbyqxmd.com/read/28153792/mosquito-borne-viruses-circulating-in-kinshasa-democratic-republic-of-the-congo
#13
Kennedy Makola Mbanzulu, Roger Wumba, Jean-Pierre Kambala Mukendi, Josué Kikana Zanga, Fortunate Shija, Thierry Lengu Bobanga, Michel Ntetani Aloni, Gerald Misinzo
BACKGROUND: Diseases caused by mosquito-borne viruses are among the most important emerging diseases that threaten human and animal health, particularly in Africa. However, little attention has been paid to these diseases in the Democratic Republic of the Congo (DRC). The present cross-sectional study was undertaken between March and May 2014 to investigate the presence of mosquito-borne viruses in mosquitoes collected from five municipalities of Kinshasa, DRC. METHODS: Mosquitoes were collected using BG-Sentinel traps and battery-powered aspirators...
January 30, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28152011/dengue-virus-serotype-2-infection-alters-midgut-and-carcass-gene-expression-in-the-asian-tiger-mosquito-aedes-albopictus
#14
Hitoshi Tsujimoto, Kathryn A Hanley, Anitha Sundararajan, Nicholas P Devitt, Faye D Schilkey, Immo A Hansen
BACKGROUND: The Asian tiger mosquito, Aedes albopictus is currently an important vector for dengue, chikungunya and Zika virus, and its role in transmission of arthropod-borne viruses (arboviruses) may increase in the future due to its ability to colonize temperate regions. In contrast to Aedes aegypti, the dominant vector of dengue, chikungunya and Zika virus, genetic responses of Ae. albopictus upon infection with an arbovirus are not well characterized. Here we present a study of the changes in transcript expression in Ae...
2017: PloS One
https://www.readbyqxmd.com/read/28150899/superinfection-interference-between-dengue-2-and-dengue-4-viruses-in-aedes-aegypti-mosquitoes
#15
Ephantus J Muturi, Eva Buckner, Jeffrey Bara
OBJECTIVE: Dengue virus consists of four antigenically distinct serotypes (DENV 1-4) that are transmitted to humans by Aedes aegypti and Ae. albopictus mosquitoes. In many dengue-endemic regions, co-circulation of two or more DENV serotypes is fairly common increasing the likelihood for exposure of the two vectors to multiple serotypes. We used a model system of DENV-2 and DENV-4 to investigate how prior exposure of Aedes aegypti to one DENV serotype affects its susceptibility to another serotype...
February 2, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28143925/adp-ribosylhydrolase-activity-of-chikungunya-virus-macrodomain-is-critical-for-virus-replication-and-virulence
#16
Robert Lyle McPherson, Rachy Abraham, Easwaran Sreekumar, Shao-En Ong, Shang-Jung Cheng, Victoria K Baxter, Hans A V Kistemaker, Dmitri V Filippov, Diane E Griffin, Anthony K L Leung
Chikungunya virus (CHIKV), an Old World alphavirus, is transmitted to humans by infected mosquitoes and causes acute rash and arthritis, occasionally complicated by neurologic disease and chronic arthritis. One determinant of alphavirus virulence is nonstructural protein 3 (nsP3) that contains a highly conserved MacroD-type macrodomain at the N terminus, but the roles of nsP3 and the macrodomain in virulence have not been defined. Macrodomain is a conserved protein fold found in several plus-strand RNA viruses that binds to the small molecule ADP-ribose...
January 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28141497/characterization-of-the-bujaru-frijoles-and-tapara-antigenic-complexes-into-the-sandfly-fever-group-and-two-unclassified-phleboviruses-from-brazil
#17
Pedro F C Vasconcelos, Joaquim Pinto Nunes Neto, William Marciel de Souza, Gustavo Olszanski Acrani, Marilia Farignoli Romeiro, Marcílio Jorge Fumagalli, Carlos Luiz Vieira, Daniele Barbosa de Almeida Medeiros, Juliana Abreu de Lima, Clayton Pereira Silva de Lima, Jedson Ferreira Cardoso, Sueli Guerreiro Rodrigues, Luiz Tadeu de Moraes Figueiredo, Sandro Patroca da Silva, Robert Tesh, Márcio Roberto Teixeira Nunes, Pedro Fernando da Costa Vasconcelos
Phlebovirus genus includes the Sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids in the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated...
January 28, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28137230/proteases-in-mosquito-borne-diseases-new-avenues-in-drug-development
#18
A Pant, R Pasupureddy, V Pande, S Seshadri, R Dixit, K C Pandey
Mosquito borne diseases continue to propagate and cause millions of deaths annually. They are caused either by protozoan parasites such as Plasmodium, Toxoplasma or by flaviviruses including Dengue and Zika. Among the proteome of such parasitic organisms, proteases play essential roles in events such as host invasion, hemoglobin hydrolysis, replication and immune evasion. Plasmepsin V (PMV), an endoplasmic reticulum resident aspartic protease of Plasmodium spp., is involved in the export of ~400 proteins containing the conserved Plasmodium Export Element motif (PEXEL)...
30, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28118506/a-high-susceptibility-to-redox-imbalance-of-the-transmissible-stages-of-plasmodium-falciparum-revealed-with-a-luciferase-based-mature-gametocyte-assay
#19
Giulia Siciliano, T R Santha Kumar, Roberta Bona, Grazia Camarda, Maria Maddalena Calabretta, Luca Cevenini, Elisabeth Davioud-Charvet, Katja Becker, Andrea Cara, David A Fidock, Pietro Alano
The goal to prevent Plasmodium falciparum transmission from humans to mosquitoes requires the identification of targetable metabolic processes in the mature (stage V) gametocytes, the sexual stages circulating in the bloodstream. This task is complicated by the apparently low metabolism of these cells, which renders them refractory to most antimalarial inhibitors and constrains the development of specific and sensitive cell-based assays. Here we identify and functionally characterize the regulatory regions of the P...
January 24, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28116530/first-mass-development-of-aedes-albopictus-diptera-culicidae-its-surveillance-and-control-in-germany
#20
Norbert Becker, Stefanie Schön, Alexandra-Maria Klein, Ina Ferstl, Ali Kizgin, Egbert Tannich, Carola Kuhn, Björn Pluskota, Artur Jöst
The Asian tiger mosquito Aedes albopictus has undergone a dramatic expansion of its range in the last few decades. Since its first detection in 2007 in Germany at the motorway A5 coming from Italy via Switzerland to Germany, it has been continuously introduced by vehicles, most probably from Italy. After a hint from an alert gardener in an allotment garden area in Freiburg, Southwest Germany, in 2015, a surveillance programme was started focusing on the garden area and adjacent areas as well as most of the cemeteries as potential infestation areas...
January 23, 2017: Parasitology Research
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