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https://www.readbyqxmd.com/read/29166538/antimicrobial-photodynamic-therapy-associated-with-conventional-endodontic-treatment-a-clinical-and-molecular-microbiological-study
#1
Caroline C da Silva, Sérgio P Chaves Júnior, Gabriela L D Pereira, Karla B F C da Fontes, Lívia A A Antunes, Helvécio C C Póvoa, Leonardo S Antunes, Natalia L P Póvoa Iorio
This study evaluated antimicrobial photodynamic therapy (aPDT) as an adjunct to endodontic treatment. Ten uniradicular teeth (control group (CG)= 4 (2 and test group (TG)= 6) with primary endodontic infections, from both genders, between 17 and 65 years old, were analyzed. Microbiological samples were collected before and after chemical-mechanical instrumentation (CMI), after aPDT (for the TG), and after the removal of the temporary restorations (second session). In TG, the aPDT was performed with 100 μg/mL methylene blue and irradiated with low power laser (InGaAIP, 660 nm; 100 mW; 40 sec) with a fiber-coupled optical laser...
November 22, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29165233/the-sec-system-protein-export-in-escherichia-coli
#2
Jennine M Crane, Linda L Randall
In Escherichia coli, proteins found in the periplasm or the outer membrane are exported from the cytoplasm by the general secretory, Sec, system before they acquire stably folded structure. This dynamic process involves intricate interactions among cytoplasmic and membrane proteins, both peripheral and integral, as well as lipids. In vivo, both ATP hydrolysis and proton motive force are required. Here, we review the Sec system from the inception of the field through early 2016, including biochemical, genetic, and structural data...
November 2017: EcoSal Plus
https://www.readbyqxmd.com/read/29163438/culture-strategies-for-isolation-of-fastidious-leptospira-serovar-hardjo-and-molecular-differentiation-of-genotypes-hardjobovis-and-hardjoprajitno
#3
Roberta T Chideroli, Daniela D Gonçalves, Suelen A Suphoronski, Alice F Alfieri, Amauri A Alfieri, Admilton G de Oliveira, Julio C de Freitas, Ulisses de Padua Pereira
The Leptospira serovar Hedjo belongs to the serogroup sejroe and this serovar is the most prevalent in bovine herds worldwide. The sejroe serogroup is the most frequently detected by serology in Brazilian cattle herds suggesting that it is due serovar Hardjo. In the molecular classification, this serovar has two genotypes: Hardjobovis and Hardjoprajitno. This serovar is as considered as fastidious pathogens, and their isolation is one of the bottlenecks in leptospirosis laboratories. In addition, its molecular characterization using genomic approaches is oftentimes not simple and time-consuming...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29160763/efficient-brain-uptake-of-piperine-and-its-pharmacokinetics-characterization-after-oral-administration
#4
Tianjing Ren, Qianwen Wang, Chenrui Li, Mengbi Yang, Zhong Zuo
1. Piperine, the major biological active component in black pepper has been associated with miscellaneous pharmacological effects, especially on central nervous system. To correlates with its neurological activity, a comprehensive pharmacokinetic profile of piperine in brain, plasma and cerebrospinal fluid after oral administration in rats was investigated in the current study. 2. It was noted that piperine could efficiently penetrate and homogeneously distribute into brain with similar pharmacokinetics profiles in each region...
November 21, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/29159827/family-based-tests-for-associating-haplotypes-with-general-phenotype-data-improving-the-fbat-haplotype-algorithm
#5
Julian Hecker, Xin Xu, F William Townes, Heide Loehlein Fier, Chris Corcoran, Nan Laird, Christoph Lange
For family-based association studies, Horvath et al. proposed an algorithm for the association analysis between haplotypes and arbitrary phenotypes when the phase of the haplotypes is unknown, that is, genotype data is given. Their approach to haplotype analysis maintains the original features of the TDT/FBAT-approach, that is, complete robustness against genetic confounding and misspecification of the phenotype. The algorithm has been implemented in the FBAT and PBAT software package and has been used in numerous substantive manuscripts...
November 21, 2017: Genetic Epidemiology
https://www.readbyqxmd.com/read/29158506/the-ikk-nf-%C3%AE%C2%BAb-signaling%C3%A2-pathway-requires-morgana-to-drive-breast-cancer-metastasis
#6
Federica Fusella, Laura Seclì, Elena Busso, Anna Krepelova, Enrico Moiso, Stefania Rocca, Laura Conti, Laura Annaratone, Cristina Rubinetto, Maurizia Mello-Grand, Vijay Singh, Giovanna Chiorino, Lorenzo Silengo, Fiorella Altruda, Emilia Turco, Alessandro Morotti, Salvatore Oliviero, Isabella Castellano, Federica Cavallo, Paolo Provero, Guido Tarone, Mara Brancaccio
NF-κB is a transcription factor involved in the regulation of multiple physiological and pathological cellular processes, including inflammation, cell survival, proliferation, and cancer cell metastasis. NF-κB is frequently hyperactivated in several cancers, including triple-negative breast cancer. Here we show that NF-κB activation in breast cancer cells depends on the presence of the CHORDC1 gene product Morgana, a previously unknown component of the IKK complex and essential for IκBα substrate recognition...
November 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/29155195/unreliability-of-the-duplex-scan-in-diagnosing-corporeal-venous-occlusive-disease-cvod-in-young-healthy-males-with-erectile-deficiency-ed
#7
Giorgio Cavallini, Carlo Maretti
OBJECTIVE: To define the role of cavernosal venous occlusive dysfunction (CVOD) as the only cause of erectile dysfunction (ED). MATERIALS AND METHODS: Patients meeting the CVOD criteria without any risk factors for organic ED were randomized into 2 groups; the end diastolic velocity (EDV), peak systolic velocity (PSV) and resistive index (RI) of their cavernosal arteries were assessed using color duplex Doppler ultrasound (CDDU) after intracavernous injection of Alprostadil 10 mcg (ICI)...
November 16, 2017: Urology
https://www.readbyqxmd.com/read/29155085/ir-spectroscopy-analysis-of-pancreatic-lipase-related-protein-2-interaction-with-phospholipids-2-discriminative-recognition-of-various-micellar-systems-and-characterization-of-plrp2-dppc-bile-salt-complexes
#8
Eduardo Mateos-Diaz, Priscila Sutto-Ortiz, Moulay Sahaka, Deborah Byrne, Hélène Gaussier, Frédéric Carrière
The interaction of pancreatic lipase-related protein 2 (PLRP2) with various micelles containing phospholipids was investigated using pHstat enzyme activity measurements, differential light scattering, size exclusion chromatography (SEC) and transmission IR spectroscopy. Various micelles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and lysophosphatidylcholine were prepared with either bile salts (sodium taurodeoxycholate or glycodeoxycholate) or Triton X-100, which are substrate-dispersing agents commonly used for measuring phospholipase activities...
November 16, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/29154875/optimization-of-pegylation-reaction-time-and-molar-ratio-of-rhg-csf-toward-increasing-bioactive-potency-of-monopegylated-protein
#9
Jamila Behi, RymHassiki, Nadia Ben Said, Balkiss Bouhaouala-Zahar, Mohamed Lamine Benkhoud
PEGylation is one of the strategies used for enhancing in vivo residence time of recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) and therefore reducing in dose frequency to better fit with patient comfort treatment. In this study, three methoxy polyethylene glycol propionaldehydes (mPEG- ALD) of 10, 20 and 30kDa MW were utilized to produce biologically active monoPEGylated rhG-CSF with enhanced molecular weight. PEGylation reactions were carried out at room temperature and pH 5.0 in the presence of cyanoborohydride and two mPEG-ALD: protein molar ratios (3:1 and 5:1)...
November 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29151916/early-changes-in-the-apparent-diffusion-coefficient-and-mmp-9-expression-of-a-cervical-carcinoma-u14-allograft-model-following-irradiation
#10
Yecai Huang, Jianming Huang, Mei Feng, Jing Ren, Kun Mi, Jia Cheng, Bing Song, Jinyi Lang
A cervical carcinoma allograft model was designed to assess the correlation between early changes in the apparent diffusion coefficient (ADC) values on diffusion-weighted magnetic resonance imaging (DW-MRI) and the expression of matrix metalloproteinase-9 (MMP-9) in tumors. BALB/c mice with U14 tumor allografts on the right rear flank were irradiated with a single 20 Gy dose. All tumor-bearing mice were subjected to DW-MRI, followed by calculation of the ADC values and characterization of the T1 and T2 relaxation time constants...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29151509/in-paroxysmal-atrial-fibrillation-patients-the-neutrophil-to-lymphocyte-ratio-is-related-to-thrombogenesis-and-more-closely-associated-with-left-atrial-appendage-contraction-than-with-the-left-atrial-body-function
#11
Yukihiro Fukuda, Mitsunori Okamoto, Shunsuke Tomomori, Hiroya Matsumura, Takehito Tokuyama, Yukiko Nakano, Yasuki Kihara
Objective The neutrophil-to-lymphocyte ratio (NLR) is an inflammation marker that can be used to detect atrial inflammatory changes, which may contribute to a reduced left atrial (LA) function and thrombosis. Our study aimed to determine whether or not the association of NLR with the LA appendage (LAA) function in relation to thrombogenesis differs from the association with the LA body function in paroxysmal atrial fibrillation (PAF) patients. Methods A total of 183 PAF patients were studied. The LA volume index, mitral flow velocity (A), and mitral annular motion velocity (A') were examined using transthoracic echocardiography...
November 20, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29151480/the-refinement-of-risk-stratification-for-atrial-thrombus-or-spontaneous-echo-contrast-in-nonvalvular-atrial-fibrillation
#12
Wenna Ning, Yang Li, Chunyan Ma, Liangxian Qiu, Bo Yu
As for nonvalvular atrial fibrillation (NVAF) patients with left atrial thrombus or spontaneous echo contrast (LAT/SEC), we evaluated the additional predictive value of serum uric acid (SUA) and Left atrial diameter (LAD) for CHADS2 and CHA2DS2-VASc, and explored the influence from the level of SUA and LAD to LAT/SEC in moderate risk group. Thus, we put forward the concept of a borderline high risk group to guide clinical anticoagulant therapy in patients with NVAF.A total of 284 NVAF patients without the history of anticoagulant prior to hospitalization were enrolled...
November 17, 2017: International Heart Journal
https://www.readbyqxmd.com/read/29151442/effects-of-short-lasting-supramaximal-intensity-exercise-on-diet-induced-increase-in-oxygen-uptake
#13
Katsunori Tsuji, Yuzhong Xu, Xin Liu, Izumi Tabata
This study was undertaken to quantify the additional increase in diet-induced oxygen uptake after exhaustive high-intensity intermittent exercise (HIIE), consisting of 6-7 bouts of 20-sec bicycle exercise (intensity: 170% V˙O2max) with a 10-sec rest between bouts. Using a metabolic chamber, the oxygen uptake of ten men was measured from 10:30 am to 07:00 am the next day on two separate days with or without HIIE, with lunch (12:00) and supper (18:00) (Diet experiment). On two other days, the oxygen uptake of six different subjects was measured from 10:30 to 16:00 with or without HIIE, but without meals (Fasting experiment)...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29151158/guidelines-to-reach-high-quality-purified-recombinant-proteins
#14
REVIEW
Carla Oliveira, Lucília Domingues
The final goal in recombinant protein production is to obtain high-quality pure protein samples. Indeed, the successful downstream application of a recombinant protein depends on its quality. Besides production, which is conditioned by the host, the quality of a recombinant protein product relies mainly on the purification procedure. Thus, the purification strategy must be carefully designed from the molecular level. On the other hand, the quality control of a protein sample must be performed to ensure its purity, homogeneity and structural conformity, in order to validate the recombinant production and purification process...
November 18, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29148804/a-nanobionic-light-emitting-plant
#15
Seon-Yeong Kwak, Juan Pablo Giraldo, Min Hao Wong, Volodymyr Koman, Tedrick Lew, Jon Ell, Mark Weidman, Rosalie Sinclair, Markita P Landry, William A Tisdale, Michael S Strano
The engineering of living plants for visible light emission and sustainable illumination is compelling because plants possess independent energy generation and storage mechanisms and autonomous self-repair. Herein, we demonstrate a plant nanobionic approach that enables exceptional luminosity and lifetime utilizing four chemically-interacting nanoparticles, including firefly luciferase conjugated silica (SNP-Luc), D-luciferin releasing poly(lactic-co-glycolic acid) (PLGA-LH2), coenzyme A functionalized chitosan (CS-CoA) and semiconductor nanocrystal phosphors for longer wavelength modulation...
November 17, 2017: Nano Letters
https://www.readbyqxmd.com/read/29147922/the-effect-of-head-to-head-competition-on-behavioural-thermoregulation-thermophysiological-strain-and-performance-during-exercise-in-the-heat
#16
Jo Corbett, Danny K White, Martin J Barwood, Christopher R D Wagstaff, Michael J Tipton, Terry McMorris, Joseph T Costello
BACKGROUND: It has been suggested that pacing is a thermoregulatory behaviour. We investigated the effect of competition on pacing, performance and thermophysiological strain during exercise in the heat and the psychological factors mediating competition effects. METHOD: Eighteen males (maximum oxygen uptake [V O 2max] 3.69 [0.44] L min(-1)) undertook a preliminary 20-km cool (wet-bulb globe temperature [WBGT] 12 °C) cycling time trial (TT) and three experimental 20-km trials (balanced order): (i) cool TT (CoolSolo); (ii) hot (WBGT 26 °C) TT (HotSolo); (iii) hot head-to-head competition (HotH2H)...
November 17, 2017: Sports Medicine
https://www.readbyqxmd.com/read/29146354/a-novel-calcium-binding-peptide-from-antarctic-krill-protein-hydrolysates-and-identification-of-binding-sites-of-calcium-peptide-complex
#17
Hu Hou, Shikai Wang, Xiao Zhu, Qiqi Li, Yan Fan, Dong Cheng, Bafang Li
Trypsin was used for preparing peptides with high calcium-binding capacity from Antarctic krill. Hydroxyapatite chromatography (HAC), size-exclusion chromatography (SEC), and reversed phase high performance liquid chromatography (RP-HPLC) were used to capture and purify calcium-binding peptides. The peptide sequence was determined to be VLGYIQIR (N- to C-terminal, MW = 960.58 Da), using LTQ Orbitrap XL. According to the results of FTIR and mass spectrometry, chelating site of calcium ions may possibly involve the carbonal or amino groups of Gln, Ile and Arg residues...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29145083/composition-and-functional-group-characterization-of-extracellular-polymeric-substances-eps-in-activated-sludge-the-impacts-of-polymerization-degree-of-proteinaceous-substrates
#18
Bin-Bin Wang, Xue-Ting Liu, Jian-Meng Chen, Dang-Cong Peng, Feng He
Characteristics of extracellular polymeric substances (EPS) in activated sludge strongly depend on wastewater substrates. Proteinaceous substrates (ProS) present in heterogeneous polymeric form are intrinsic and important parts of wastewater substrates for microorganisms in activated sludge systems. However, correlations between ProS and characteristics of EPS are scarce. This study systematically explored the impacts of monomeric (Mono-), low polymeric (LoP-) and high polymeric (HiP-) ProS on compositions and functional groups of EPS in activated sludge...
November 3, 2017: Water Research
https://www.readbyqxmd.com/read/29144630/is-circumferential-urethral-mobilisation-an-overdo-a-prospective-outcome-analysis-of-dorsal-onlay-and-dorso-lateral-onlay-bmgu-for-anterior-urethral-strictures
#19
Gaurav Prakash, Bhupendra Pal Singh, Rahul Janak Sinha, Ankur Jhanwar, Satyanarayan Sankhwar
INTRODUCTION: For dorsal onlay graft placement, unilateral urethral mobilization is less invasive than standard circumferential urethral mobilization. Apart from success in terms of patency of urethra, other issues like sexual function, overall quality of life and patient satisfaction remain important issues while comparing outcomes of urethroplasty. AIM: To prospectively compare the objective as well as subjective outcomes of two approaches. MATERIALS AND METHODS: Between July 2011 and January 2015, 136 adult males having anterior urethral stricture with urethral lumen ≥ 6 Fr...
November 19, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/29144402/characterization-of-non-derivatized-cellulose-samples-by-size-exclusion-chromatography-in-tetrabutylammonium-fluoride-dimethylsulfoxide-tbaf-dmso
#20
Jérémy Rebière, Antoine Rouilly, Vanessa Durrieu, Frédéric Violleau
This paper deals with the use of tetrabutylammonium fluoride/dimethylsulfoxide (TBAF/DMSO) to characterize the molar mass distribution of non-derivatized cellulosic samples by size exclusion chromatography (SEC). Different cellulose samples with various average degree of polymerization (DP) were first solubilized in this solvent system, with increasing TBAF rates, and then analyzed by SEC coupled to a refractive index detector (RID), using DMSO as mobile phase. The Molar Masses (MM) obtained by conventional calibration were then discussed and compared with suppliers' data and MM determined by viscosimetry measurements...
November 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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