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https://www.readbyqxmd.com/read/28543032/influence-of-different-wild-garlic-allium-ursinum-extracts-on-the-gastrointestinal-system-spasmolytic-antimicrobial-and-antioxidant-properties
#1
Dragana R Pavlović, Milica Veljković, Nikola M Stojanović, Marija Gočmanac-Ignjatović, Tatjana Mihailov-Krstev, Suzana Branković, Dušan Sokolović, Mirjana Marčetić, Niko Radulović, Mirjana Radenković
OBJECTIVES: As there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. METHODS: Wild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay...
May 23, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28537028/enhanced-larvicidal-antibacterial-and-photocatalytic-efficacy-of-tio2-nanohybrids-green-synthesized-using-the-aqueous-leaf-extract-of-parthenium-hysterophorus
#2
Keerthika Thandapani, Manikandan Kathiravan, Elangovan Namasivayam, Indira Arulselvi Padiksan, Geetha Natesan, Manish Tiwari, Benelli Giovanni, Venkatachalam Perumal
Titanium dioxide nanoparticles are emerging as a biocompatible nanomaterial with multipurpose bioactivities. In this study, titanium dioxide (TiO2) nanoparticles were effectively synthesized using the aqueous leaf extracts of Parthenium hysterophorus prepared by microwave irradiation. TiO2 nanoparticles were fabricated by treating the P. hysterophorus leaf extracts with the TiO4 solution. Biologically active compounds such as alcohols, phenols, alkanes, and fluoroalkanes were involved in bioreduction of TiO4 into TiO2...
May 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28536926/structural-changes-of-gut-microbiota-in-parkinson-s-disease-and-its-correlation-with-clinical-features
#3
Wei Li, Xiaoli Wu, Xu Hu, Tao Wang, Shan Liang, Yunfeng Duan, Feng Jin, Bin Qin
The aim of this study was to compare the structure of gut microbiota in Parkinson's disease (PD) patients and healthy controls; and to explore correlations between gut microbiota and PD clinical features. We analyzed fecal bacterial composition of 24 PD patients and 14 healthy volunteers by using 16S rRNA sequencing. There were significant differences between PD and healthy controls, as well as among different PD stages. The putative cellulose degrading bacteria from the genera Blautia (P=0.018), Faecalibacterium (P=0...
May 20, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28535686/development-and-characterization-of-a-stable-adhesive-bond-between-a-poly-dimethylsiloxane-catheter-material-and-a-bacterial-biofilm-resistant-acrylate-polymer-coating
#4
Bonnie J Tyler, Andrew Hook, Andreas Pelster, Paul Williams, Morgan Alexander, Heinrich F Arlinghaus
Catheter associated urinary tract infections are the most common health related infections worldwide, contributing significantly to patient morbidity and mortality and increased health care costs. To reduce the incidence of these infections, new materials that resist bacterial biofilm formation are needed. A composite catheter material, consisting of bulk poly(dimethylsiloxane) (PDMS) coated with a novel bacterial biofilm resistant polyacrylate [ethylene glycol dicyclopentenyl ether acrylate (EGDPEA)-co-di(ethyleneglycol) methyl ether methacrylate (DEGMA)], has been proposed...
May 23, 2017: Biointerphases
https://www.readbyqxmd.com/read/28534309/environmental-monitoring-and-assessment-of-antibacterial-metabolite-producing-actinobacteria-screened-from-marine-sediments-in-south-coastal-regions-of-karnataka-india
#5
Sinosh Skariyachan, Shruthi Garka, Sushmitha Puttaswamy, Shobitha Shanbhogue, Raksha Devaraju, Rajeswari Narayanappa
Assessment of the therapeutic potential of secondary metabolite producing microorganisms from the marine coastal areas imparts scope and application in the field of environmental monitoring. The present study aims to screen metabolites with antibacterial potential from actionbacteria associated with marine sediments collected from south coastal regions of Karnataka, India. The actinobacteria were isolated and characterized from marine sediments by standard protocol. The metabolites were extracted, and antibacterial potential was analyzed against eight hospital associated bacteria...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28523199/de-novo-pten-mutation-in-a-young-boy-with-cutaneous-vasculitis
#6
Angela Mauro, Ebun Omoyinmi, Neil James Sebire, Angela Barnicoat, Paul Brogan
Phosphatase and tensin homolog (PTEN) is the protein encoded by the PTEN gene (10q23.3). PTEN mutations are related to a variety of rare diseases referred to collectively as PTEN hamartoma tumor syndromes (PHTS), which include Cowden Syndrome, Bannayan-Riley-Ruvalcaba syndrome, Proteus Syndrome, and Proteus-like syndrome. These diseases are associated with an increased risk of malignancy and for this reason an accurate and early diagnosis is essential in order to institute cancer surveillance. PTEN is a regulator of growth and homeostasis in immune system cells, although there are limited data describing immune dysregulation caused by PTEN mutations...
2017: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/28516867/the-link-between-proteus-mirabilis-environmental-factors-and-autoantibodies-in-rheumatoid-arthritis
#7
REVIEW
Taha Rashid, Alan Ebringer, Clyde Wilson
Rheumatoid arthritis (RA) is a relatively common and potentially disabling immune-mediated inflammatory systemic disease, predominantly affecting women and characterised by multiple small joint arthritis. Extensive data supports the roles of genetic, environmental and microbial factors in the triggering and development of this disease. Proteus mirabilis is considered as the main microbial culprit in the causation of RA. The evidence for the role of these microbes in RA and their links with commonly associated autoantibodies such as rheumatoid factors and anti-citrullinated peptide antibodies have been elucidated together with their relations with some of the non-microbial environmental factors which have been implicated in the aetiopathogenesis of RA...
May 8, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/28510991/development-of-a-panel-of-recombinase-polymerase-amplification-assays-for-detection-of-common-bacterial-urinary-tract-infection-pathogens
#8
Balakrishnan Raja, Heather J Goux, Archana Marapadaga, Sri Rajagopalan, Katerina Kourentzi, Richard C Willson
AIMS: To develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (RPA) assays for detection of common bacterial urinary tract infection (UTI) pathogens. METHODS AND RESULTS: The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Enterococcus faecalis. All five RPAs required reaction times of under 12 minutes to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with high concentrations of non-target bacterial genomic DNA...
May 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28508939/-the-worlds-of-gods-in-medicine
#9
REVIEW
A Karenberg
BACKGROUND: A number of designations for diseases, medicines and human body structures derive from classical mythology. To date, these eponyms have not been systematically investigated. OBJECTIVES: This paper provides an overview of this fringe component of medical vocabulary, looks at the history of several terms and formulates hypotheses as to why such creative etymologies have come into being. MATERIALS AND METHODS: In addition to relevant texts on ancient mythology, a variety of medical textbooks from the early modern period were analyzed...
May 16, 2017: Zeitschrift Für Rheumatologie
https://www.readbyqxmd.com/read/28506940/cool-ami-eu-pilot-trial-a-multicentre-prospective-randomised-controlled-trial-to-assess-cooling-as-an-adjunctive-therapy-to-percutaneous-intervention-in-patients-with-acute-myocardial-infarction
#10
Marko Noc, David Erlinge, Aleksandar N Neskovic, Srdjan Kafedzic, Béla Merkely, Endre Zima, Misa Fister, Milovan Petrović, Milenko Čanković, Gábor Veress, Peep Laanmets, Teele Pern, Vladan Vukcevic, Vladimir Dedovic, Beata Średniawa, Andrzej Świątkowski, Thomas R Keeble, John R Davies, Alexandra-Maria Warenits, Göran Olivecrona, Jan Zbigniew Peruga, Michal Ciszewski, Ivan Horvath, Istvan Edes, Gergely Gyorgy Nagy, Daniel Aradi, Michael Holzer
AIMS: We aimed to investigate the rapid induction of therapeutic hypothermia using the ZOLL Proteus Intravascular Temperature Management System in patients with anterior ST-elevation myocardial infarction (STEMI) without cardiac arrest. METHODS AND RESULTS: A total of 50 patients were randomised; 22 patients (88%; 95% confidence interval [CI]: 69-97%) in the hypothermia group and 23 patients (92%; 95% CI: 74-99) in the control group completed cardiac magnetic resonance imaging at four to six days and 30-day follow-up...
May 17, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28503342/phenotypic-detection-and-antibiogram-of-%C3%AE-lactamase-producing-proteus-species-in-a-tertiary-care-hospital-india
#11
N Pal, S Hooja, R Sharma, R K Maheshwari
BACKGROUND: Proteus species cause a variety of community- and hospital-acquired illnesses. Synthesis of β-lactamases is the predominant mechanism for resistance to β-lactam antibiotics. Among the β-lactamases, extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases are the most common. AIM: The objective of this study was to determine the occurrence of ESBL and AmpC β-lactamases in Proteus species among various clinical isolates at a tertiary care hospital, India...
September 2016: Annals of Medical and Health Sciences Research
https://www.readbyqxmd.com/read/28502730/overgrowth-syndromes-caused-by-somatic-variants-in-the-phosphatidylinositol-3-kinase-akt-mammalian-target-of-rapamycin-pathway
#12
REVIEW
Gozde Akgumus, Fengqi Chang, Marilyn M Li
Somatic variants have been well described in tumorigenesis; however, they are only recently appreciated in other human disorders, such as mosaic overgrowth syndromes. Although overgrowth is a manifestation in many genetic syndromes, not all overgrowth syndromes are inherited. Mosaic somatic variants have been lately described in several overgrowth disorders, such as Proteus syndrome, CLOVES (congenital, lipomatous, overgrowth, vascular malformations, epidermal nevi, and spinal/skeletal anomalies and/or scoliosis) syndrome, megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome, and megalencephaly-capillary malformation-polymicrogyria syndrome...
May 11, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28502725/molecular-diagnosis-of-mosaic-overgrowth-syndromes-using-a-custom-designed-next-generation-sequencing-panel
#13
Fengqi Chang, Liu Liu, Erica Fang, Guangcheng Zhang, Tiansheng Chen, Kajia Cao, Yanchun Li, Marilyn M Li
Recent studies have discovered a group of overgrowth syndromes, such as congenital lipomatous overgrowth with vascular, epidermal, and skeletal anomalies (CLOVES) syndrome, Proteus syndrome, and megalencephaly-capillary malformation-polymicrogyria (MCAP) syndrome, are caused by somatic activating variants in the genes involved in the phosphatidylinositol 3-kinase/AKT/mechanistic target of rapamycin pathway. Because of the low-abundance nature of these pathogenic variants, Sanger sequencing often yields negative results...
May 11, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28498641/quaternary-ammonium-salts-substituted-by-5-phenyl-1-3-4-oxadiazole-2-thiol-as-novel-antibacterial-agents-with-low-cytotoxicity
#14
Chun-Hua Wang, Xian-Rui Xie, Wen-Shuai Liu, Gui-Ge Hou, Ju-Feng Sun, Feng Zhao, Wei Cong, Hong-Juan Li, Wen-Yu Xin
Twenty-one novel 5-phenyl-1,3,4-oxadiazole-2-thiol (POT) substituted N-hydroxyethyl quaternary ammonium salts (6a-g, 7a-g, 8a-g) were prepared and characterized by FTIR, NMR and elemental analysis. Compounds 6a, 6c and 8a were confirmed by X-ray single-crystal diffraction. They display the unsurpassed antibacterial activity against S. aureus, α-H-tococcus, E. coli, P. aeruginosa, Proteus vulgaris, Canidia Albicans, especially 6g, 7g, 8g with dodecyl group. Compounds 8a-d with N,N-dihydroxyethyl and POT groups display unsurpassed antibacterial activity and nontoxicity...
May 12, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28497048/antimicrobial-resistance-patterns-in-women-with-positive-urine-culture-does-menopausal-status-make-a-significant-difference
#15
Pawel Miotla, Katarzyna Romanek-Piva, Michal Bogusiewicz, Ewa Markut-Miotla, Aneta Adamiak, Andrzej Wróbel, Małgorzata Zebrowska, Sara Wawrysiuk, Katarzyna Mendyk, Ewa Rechberger, Agata Jakubczak, Tomasz Rechberger
Aim. Urinary tract infection (UTI) is considered one of the most common bacterial infections in women. The aim of this study was to investigate the types of uropathogens present, as well as the degree of antimicrobial drug resistance seen among premenopausal (n = 2748) and postmenopausal (n = 1705) women with uncomplicated UTI. Methods. Urinary samples (n = 4453) collected from women with UTI were analyzed in terms of uropathogens present. These were considered as positive if bacterial growth was ≥10(5) colony forming units (CFUs)/mL...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28493774/urinary-catheter-related-visits-to-the-emergency-department-and-implications-for-community-services
#16
Tiziana Ansell, Danielle Harari
Thousands of patients live with urinary catheters and the potential complications deriving from long-term use. Disjointed community services may result in patients attending the emergency department (ED) to manage catheter complications. AIM: to conduct a service review of catheterised patients attending the ED of a large London hospital; to describe incidence, reasons for attendance and cost to inform future planning for out-of-hospital care. METHOD: a catheter collaborative, consisting of multidisciplinary health professionals and patients, formulated survey questions...
May 11, 2017: British Journal of Nursing: BJN
https://www.readbyqxmd.com/read/28491218/bacterial-agents-of-the-discharging-middle-ear-among-children-seen-at-the-university-of-nigeria-teaching-hospital-enugu
#17
Gideon Chukwudalu Ilechukwu, Chioma Azuka Ilechukwu, Agozie Chukwunedum Ubesie, Ijeoma Okoroafor, Basil Chukwuemeka Ezeanolue, Ngozi Chinyelu Ojinnaka
INTRODUCTION: Discharging middle ear continues to be one of the commonest problems seen in the developing world. There is an ever growing need to carry out studies periodically to determine the common bacterial agents responsible for discharging otitis media and their antibiotic sensitivity especially in set-ups characterized with minimal laboratory services. The study sought to determine the common bacterial agents causing discharging middle ear among children presenting at the University of Nigeria Teaching Hospital, Enugu and their sensitivity to the commonly available antibiotics...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28490063/characterisation-of-a-thiamine-diphosphate-dependent-alpha-keto-acid-decarboxylase-from-proteus-mirabilis-jn458
#18
Biying Wang, Yajun Bai, Taiping Fan, Xiaohui Zheng, Yujie Cai
Alpha-keto acid decarboxylases can convert keto acids to their corresponding aldehydes, which are often volatile aroma compounds. The gene encoding α-keto acid decarboxylase in Proteus mirabilis JN458 was cloned, and the enzyme overexpressed in Escherichia coli BL21 (DE3), purified in high yield, and characterised. The molecular weight is 62.291kDa by MALDI-TOF MS, and optimum activity at pH 6.0 and 40-50°C. The enzyme is a typical decarboxylase, dependent on thiamine diphosphate and Mg(2+) as cofactors. For the decarboxylation reaction, the enzyme displayed a broad substrate range...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28485708/molecular-study-of-carbapenemase-genes-in-clinical-isolates-of-enterobacteriaceae-resistant-to-carbapenems-and-determining-their-clonal-relationship-using-pulsed-field-gel-electrophoresis
#19
Fereshteh Shahcheraghi, Mohammad Mehdi Aslani, Hassan Mahmoudi, Zahra Karimitabar, Hamid Solgi, Abbas Bahador, Mohammad Yousef Alikhani
PURPOSE: Enterobacteriaceae is a large family of Gram-negative bacteria that are considered as normal gut flora. They are the most common human pathogens. The main objective of this study was to investigate the carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenem antibiotics and determine their clonal relationship using pulsed-field gel electrophoresis (PFGE). METHODOLOGY: In the present study, bacteria were isolated and identified via conventional biochemical tests and API 20NE...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28484348/the-most-frequent-isolates-from-outpatients-with-urinary-tract-infection
#20
Sabina Mahmutovic Vranic, Nadira Zatric, Velma Rebic, Mufida Aljicevic, Amila Abdulzaimovic
OBJECTIVES: The main objective of the study was to identify the most frequent isolates from female outpatients with urinary tract infection. MATERIAL AND METHODS: The retrospective study was performed at the Department of Microbiology, Parasitology and Virology Faculty of Medicine, University of Sarajevo in period of 2015. The most frequent isolates were determined in 405 first urine samples from female outpatients of Hrasno community in Sarajevo, B&H. Identification of isolates was performed by the standard microbiological testing and the standard methods of descriptive statistics as well...
March 2017: Materia Socio-medica
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