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https://www.readbyqxmd.com/read/28435889/multidrug-resistance-salmonella-genomic-island-1-in-a-morganella-morganii-subsp-morganii-human-clinical-isolate-from-france
#1
Eliette Schultz, Olivier Barraud, Jean-Yves Madec, Marisa Haenni, Axel Cloeckaert, Marie-Cécile Ploy, Benoît Doublet
Salmonella genomic island 1 (SGI1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous Salmonella enterica serovars and also in Proteus mirabilis. A serious threat to public health was revealed in the recent description in P. mirabilis of a SGI1-derivative multidrug resistance island named PGI1 (Proteus genomic island 1) carrying extended-spectrum-β-lactamase (ESBL) and metallo-β-lactamase resistance genes, blaVEB-6 and blaNDM-1, respectively...
March 2017: MSphere
https://www.readbyqxmd.com/read/28434745/hot-topic-16s-rrna-gene-sequencing-reveals-the-microbiome-of-the-virgin-and-pregnant-bovine-uterus
#2
S G Moore, A C Ericsson, S E Poock, P Melendez, M C Lucy
We tested the hypothesis that the uterus of virgin heifers and pregnant cows possessed a resident microbiome by 16S rRNA gene sequencing of the virgin and pregnant bovine uterus. The endometrium of 10 virgin heifers in estrus and the amniotic fluid, placentome, intercotyledonary placenta, cervical lumen, and external cervix surface (control) of 5 pregnant cows were sampled using aseptic techniques. The DNA was extracted, the V4 hypervariable region of the 16S rRNA gene was amplified, and amplicons were sequenced using Illumina MiSeq technology (Illumina Inc...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28411141/formulation-of-carbapenems-loaded-gold-nanoparticles-to-combat-multi-antibiotic-bacterial-resistance-in-vitro-antibacterial-study
#3
Mohamed A Shaker, Mona I Shaaban
Despite the fact that carbapenems (powerful β-lactams antibiotics) were able to fight serious infectious diseases, nowadays the spread of carbapenems-resistant bacteria is considered the main challenge in antibacterial therapy. In this study, we focused on evaluating the surface conjugation of carbapenems (imipenem and meropenem) with gold nanoparticles as a delivering strategy to specifically and safely maximize their therapeutic efficacy while destroying the developing resistance of the pathogens. Different particle size formulae (35, 70 and 200nm) were prepared by citrate reduction method...
April 11, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28408060/bacterial-epidemiology-and-antimicrobial-resistance-profiles-of-urinary-specimens-of-the-elderly
#4
F Gravey, G Loggia, A de La Blanchardière, V Cattoir
OBJECTIVE: Although urinary tract infections are the second leading cause of infections among patients aged above 65 years, data on bacterial epidemiology of urinary specimens in these patients is scarce. Our aim was to describe the main bacterial species found at significant levels in urine specimens of the elderly and to determine their antimicrobial resistance profiles. METHODS: From October 2012 to October 2015, all urinary specimens (catheter-related or not) received at the laboratory of microbiology of the university hospital of Caen (France) were retrospectively studied...
April 10, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/28401466/antibacterial-effects-of-lactobacillus-isolates-of-curd-and-human-milk-origin-against-food-borne-and-human-pathogens
#5
Chetan Sharma, Brij Pal Singh, Nishchal Thakur, Sachin Gulati, Sanjolly Gupta, Santosh Kumar Mishra, Harsh Panwar
This study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. Identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific PCR and 16S rRNA-based sequencing. These strains belonged to five species, Lactobacillus casei, L. delbrueckii, L. fermentum, L. plantarum, and L. pentosus. Antibacterial activities of cell-free supernatants (CFSs) of all the Lactobacillus isolates were estimated through standard agar-well diffusion assay, against commonly occurring food-borne and clinically important human pathogens...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28401074/proteus-syndrome-with-arteriovenous-malformation
#6
Ali Asilian, Atefeh Sadat Kamali, Nabet Tajmir Riahi, Neda Adibi, Fatemeh Mokhtari
Proteus syndrome is a rare sporadic disorder that appears with localized macrosomia, congenital lipomatosis, and slow flow vascular malformations, connective tissue nevus, and epidermal nevus. There are usually some manifestations at birth. The vascular abnormalities that have been reported in Proteus syndrome are capillary and slow flow venous malformation. We report a case of a 10-year-old boy with confirmed Proteus syndrome characterized by high flow vascular malformation (arteriovenous [AV] malformation) unlike the usual vascular malformations seen in this syndrome...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28399332/determination-of-spoilage-microbiota-of-pacific-white-shrimp-during-ambient-and-cold-storage-using-next-generation-sequencing-and-culture-dependent-method
#7
Sheng-Ping Yang, Jing Xie, Yun-Fang Qian
This study was conducted to determine the initial and spoilage microbiota of Pacific white shrimp during ambient and cold storage using next-generation sequencing (NGS) and a culture-dependent method. The quality changes were also evaluated by sensory analysis and total volatile basic nitrogen (TVB-N) values. After 1 d of storage, the psychrotrophic bacteria were only 5.97 log CFU/g, accounting for 1.1% of the mesophilic bacteria counts (7.94 log CFU/g). The psychrotrophic bacteria counts exceeded the counts of mesophilic bacteria for shrimp stored at 4 °C after 6 d of storage, indicating that psychrotrophic bacteria became predominant...
April 11, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28395682/wound-infection-secondary-to-snakebite
#8
M Wagener, M Naidoo, C Aldous
BACKGROUND: Snakebites can produce severe local and systemic septic complications as well as being associated with significant overall morbidity and even mortality. OBJECTIVE: A prospective audit was undertaken to determine the bacterial causation of wound infection secondary to snakebite, and attempt to quantify the burden of disease. METHODS: The audit was undertaken at Ngwelezane Hospital, which provides both regional and tertiary services for north-eastern KwaZulu-Natal Province, South Africa, over a 4-month period...
March 29, 2017: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/28393548/hiv-and-hcv-infection-among-opiate-dependent-patients-and-methadone-doses-the-proteus-study
#9
Carlos Roncero, Daniel Fuster, Raul Felipe Palma-Álvarez, Laia Rodriguez-Cintas, Nieves Martinez-Luna, F Javier Álvarez
Human immunodeficiency virus (HIV) and hepatitis C virus (HCV) are prevalent infections in opiate-dependent patients. Opiate replacement treatment (ORT) with methadone or buprenorphine is associated with several important outcomes among patients with opiate dependence. However, little is known about outcomes in patients with HIV and/or HCV infections that are in ORT. Also, it is not well established whether the presence of HCV or HIV infection could be associated with higher methadone doses. This paper reanalyzes the database of PROTEUS study, using two principal variables: methadone dose and presence of HIV and/or HCV infection...
April 9, 2017: AIDS Care
https://www.readbyqxmd.com/read/28390962/in-vitro-activity-of-fosfomycin-trometamol-and-other-oral-antibiotics-against-multi-drug-resistant-uropathogens
#10
Maria Lina Mezzatesta, Giulia La Rosa, Gaetano Maugeri, Tiziana Zingali, Carla Caio, Andrea Novelli, Stefania Stefani
Clinical midstream and urinary catheter isolates (n=106) of extended-spectrum β-lactamase (ESBL)-positive Escherichia coli, carbapenemase-producing Klebsiella pneumoniae, Proteus mirabilis and meticillin-resistant Staphylococcus saprophyticus were tested against fosfomycin using the agar dilution method, the broth microdilution method and the gradient test described by the Clinical and Laboratory Standards Institute. Nitrofurantoin, co-trimoxazole, amoxicillin/clavulanic acid, cefuroxime, levofloxacin and ciprofloxacin were tested using the gradient test alone...
April 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28389893/toxicity-of-herbal-extracts-used-in-ethno-veterinary-medicine-and-green-encapsulated-zno-nanoparticles-against-aedes-aegypti-and-microbial-pathogens
#11
Balan Banumathi, Baskaralingam Vaseeharan, Ramachandran Ishwarya, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
Dengue and chikungunya are arboviral diseases mainly vectored by the mosquito Aedes aegypti. Presently, there is no treatment for these viral diseases and their prevention is still based on vector control measures. Nanopesticides fabricated using herbal extracts as reducing and capping agents currently represent an excellent platform for pest control. In this scenario, the present study assessed the acute toxicity of seven plants employed in ethno-veterinary medicine of southern India, as well as the green synthesis of zinc oxide nanoparticles, on third-instar larvae of A...
April 7, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28384626/complete-genome-sequence-of-proteus-mirabilis-phage-ppm_01-isolated-from-raw-sewage
#12
Ira Aryani Wirjon, Nyok-Sean Lau, Yahya Mat Arip
OBJECTIVES: Phage pPM_01 was previously isolated from a raw sewage treatment facility located in Batu Maung, Penang, Malaysia, and it was highly lytic against Proteus mirabilis, which causes urinary tract infections in humans. In this paper, we characterize the biology and complete genome sequence of the phage. METHODS AND RESULTS: Transmission electron microscopy revealed phage pPM_01 to be a siphovirus (the first reported virus to infect P. mirabilis), with its complete genome sequence successfully determined...
April 7, 2017: Intervirology
https://www.readbyqxmd.com/read/28384093/antimicrobial-susceptibility-monitoring-of-bacterial-pathogens-isolated-from-urinary-tract-infections-in-dogs-and-cats-across-europe-compath-results
#13
Hilde Moyaert, Ian Morrissey, Anno de Jong, Farid El Garch, Ulrich Klein, Carolin Ludwig, Julien Thiry, Myriam Youala
ComPath is a pan-European antimicrobial surveillance program collecting bacterial pathogens from dogs and cats not recently exposed to antimicrobials. We present minimum inhibitory concentration data obtained using Clinical and Laboratory Standards Institute methodology for 616 urinary tract infection (UTI) isolates collected between 2008 and 2010. In both dogs and cats, the most common pathogen was Escherichia coli (59.8% and 46.7%, respectively). Antimicrobial activity against E. coli in dogs and cats was similar with fluoroquinolone and trimethoprim/sulfamethoxazole susceptibility >90%...
April 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28379126/-community-acquired-urinary-tract-infections-in-older-adults
#14
Alberto Francisco Leoni, Aida Monterisi, Paula G Acuña
Background Community acquired urinary tract infections (caUTI) in adults are common ailments. Older adults are prone to infectious diseases, diagnosis can be difficult, their etiologic and antimicrobial resistance are poorly known. Objectives To evaluate the incidence discriminated by sex, symptoms, and to determine pathogens and their antimicrobial resistance rate. Methods A retrospective, descriptive and comparative study. We analyze uricultures among 2013 in the Hospital Nacional de Clinicas (Córdoba-Argentina)...
2017: Revista de la Facultad de Ciencias Médicas
https://www.readbyqxmd.com/read/28377345/-expression-of-proteus-mirabilis-polyphosphate-kinase-and-preparation-of-its-polyclonal-antibodies
#15
Liang Peng, Jing-Yi Ou, Jia-Yun Pan, Cong Deng, Jing-Hong Chen, Hong Cao
OBJECTIVE: To express and purify polyphosphate kinase (PPK) from Proteus mirabilis and prepare the polyclonal antibody against PPK. METHODS: The antigenicity and hydrophobicity of PPK were analyzed using software. The N-terminal conservative sequence containing 309 amino acids was selected as the target peptide, and its corresponding gene sequence with modification based on prokaryotic cells-preferred codon was synthesized and inserted into plasmid pET28b(+). The constructed recombinant plasmid was transformed into Escherichia coli BL21 (DE3) and induced with IPTG...
March 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28371761/bioremediation-of-storage-tank-bottom-sludge-by-using-a-two-stage-composting-system-effect-of-mixing-ratio-and-nutrients-addition
#16
Ali Koolivand, Mohammad Sadegh Rajaei, Mohammad Javad Ghanadzadeh, Reza Saeedi, Hamid Abtahi, Kazem Godini
The effect of mixing ratio and nutrients addition on the efficiency of a two-stage composting system in removal of total petroleum hydrocarbons (TPH) from storage tank bottom sludge (STBS) was investigated. The system consisted of ten windrow piles as primary composting (PC) followed by four in-vessel reactors as secondary composting (SC). Various initial C/N/P and mixing ratios of STBS to immature compost (IC) were examined in the PC and SC for 12 and 6weeks, respectively. The removal rates of TPH in the two-stage system (93...
March 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28368860/gallbladder-microbiota-variability-in-colombian-gallstones-patients
#17
Ariel Antonio Arteta, Hernan Carvajal-Restrepo, Miryan Margot Sánchez-Jiménez, Sergio Diaz-Rodriguez, Nora Cardona-Castro
INTRODUCTION: Gallbladder stones are a very frequently occurring condition. Despite bile bactericidal activity, many bacteria have been detected inside the gallbladder, and gallstones facilitate their presence. Between 3% and 5% of the patients with Salmonella spp. infection develop the carrier stage, with the bacteria persisting inside the gallbladder, shedding bacteria in their feces without signs of infection. The aim of this study was to isolate bacteria from Colombian patients with gallstones, using standard culturing methods, and to identify Salmonella spp...
March 31, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28367445/antimicrobial-activity-and-chemical-composition-of-kp%C3%A3-t%C3%A3-kp%C3%A3-t%C3%A3-a-starter-of-benin-traditional-beer-tchoukoutou
#18
Christine N'tcha, Haziz Sina, Adéchola Pierre Polycarpe Kayodé, Joachim D Gbenou, Lamine Baba-Moussa
The aim of this study was to investigate the antibacterial effect of the crude starter "kpètè-kpètè" and lactic acid bacteria used during the production of "tchoukoutou." To achieve this, a total of 11 lactic acid bacteria and 40 starter samples were collected from four communes. The samples were tested on 29 gram + and - strains by disk diffusion method. The minimum inhibitory and bactericidal concentrations of starter and lactic acid bacteria were determined by conventional methods. Organic acids, sugar, and volatile compounds were determined using the HPLC method...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28367029/biofilm-colonization-in-chronic-treatment-refractory-infections-presenting-with-discharging-sinuses-a-study-in-a-tertiary-care-hospital-of-eastern-india
#19
Asmita De, Hirak Jyoti Raj, Jayeeta Haldar, Poulami Mukherjee, Prasanta Kumar Maiti
INTRODUCTION: Treatment refractory chronic recurrent infections mean those chronic infections which recur by same causal agents with similar drug responsiveness after apparent relief following full course of recommended antimicrobial management. MATERIALS AND METHODS: Fifty different samples were collected from patients with chronic surgical site infections, laparoscopic port site infections, anal fistula, mesh hernioplasty, chronic dacryocystitis, chronic osteomyelitis, and chronic burn wounds...
April 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28365882/proteus-a-random-forest-classifier-to-predict-disorder-to-order-transitioning-binding-regions-in-intrinsically-disordered-proteins
#20
Sankar Basu, Fredrik Söderquist, Björn Wallner
The focus of the computational structural biology community has taken a dramatic shift over the past one-and-a-half decades from the classical protein structure prediction problem to the possible understanding of intrinsically disordered proteins (IDP) or proteins containing regions of disorder (IDPR). The current interest lies in the unraveling of a disorder-to-order transitioning code embedded in the amino acid sequences of IDPs/IDPRs. Disordered proteins are characterized by an enormous amount of structural plasticity which makes them promiscuous in binding to different partners, multi-functional in cellular activity and atypical in folding energy landscapes resembling partially folded molten globules...
April 1, 2017: Journal of Computer-aided Molecular Design
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