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https://www.readbyqxmd.com/read/28820412/-to-the-state-of-the-fetoplacental-complex-in-women-with-the-threat-of-abortion
#1
L Rzaguliyeva, Z Osmanli, S Akperbekova, N Meybalizade
The goal is a comprehensive study of the morphofunctional state of the mother-placenta-fetus system in placental insufficiency and infection. 250 pregnant and puerperas for the period 2008-2014 were examined. Detection of bacterial flora was carried out in 35 pregnant women aged 20.4±0.8 years with the threat of abortion from an early gestation period and a prolonged persistent infection of the genitals, bacterial vaginosis. Methods are applied: culture, ion chromatography, gas-liquid chromatography with mass spectrometry...
July 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28820112/prevalence-study-of-plasmid-mediated-ampc-%C3%AE-lactamases-in-enterobacteriaceae-lacking-inducible-ampc-from-saudi-hospitals
#2
Baha Abdalhamid, Samar Albunayan, Alaa Shaikh, Nasreldin Elhadi, Reem Aljindan
PURPOSE: Enterobacteriaceae encoding plasmid-mediated AmpC (pAmpC) β-lactamases confer resistance to the third generation cephalosporins. pAmpC association with extended spectrum β-lactamases (ESBLs), plasmid-mediated quinolone resistance (PMQR) and aminoglycoside modifying enzymes (AMEs) is well documented. There are limited data regarding the epidemiology and clinical significance of pAmpC in Saudi Arabia. This study aimed to determine the prevalence of pAmpC and its coexistence with ESBLs, PMQR and AMEs in Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis isolates in Saudi hospitals from January to December 2015...
August 18, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28817637/genetic-diversity-of-the-o-antigens-of-proteus-species-and-the-development-of-a-suspension-array-for-molecular-serotyping
#3
Xiang Yu, Agnieszka Torzewska, Xinjie Zhang, Zhiqiu Yin, Dominika Drzewiecka, Hengchun Cao, Bin Liu, Yuriy A Knirel, Antoni Rozalski, Lei Wang
Proteus species are well-known opportunistic pathogens frequently associated with skin wound and urinary tract infections in humans and animals. O antigen diversity is important for bacteria to adapt to different hosts and environments, and has been used to identify serotypes of Proteus isolates. At present, 80 Proteus O-serotypes have been reported. Although the O antigen structures of most Proteus serotypes have been identified, the genetic features of these O antigens have not been well characterized. The O antigen gene clusters of Proteus species are located between the cpxA and secB genes...
2017: PloS One
https://www.readbyqxmd.com/read/28809953/monitoring-health-and-reproductive-status-of-olms-proteus-anguinus-by-ultrasound
#4
Susanne Holtze, Maja Lukač, Ivan Cizelj, Frank Mutschmann, Claudia Anita Szentiks, Dušan Jelić, Robert Hermes, Frank Göritz, Stanton Braude, Thomas Bernd Hildebrandt
The olm (Proteus anguinus) is a troglomorphic, neotenous amphibian with extraordinary life expectancy and unique adaptations that deserve further investigation. A low reproductive rate and habitat decline render it threatened by extinction. Establishing captive populations for maintenance and artificial breeding may one day become crucial to the species. Longitudinal, in-vivo assessment of inner organs is invaluable to our understanding of reproductive physiology, health, and behavior. Using ultrasound, we measured heart rate and assessed health and reproductive status of 13 captive olms at Zagreb Zoo...
2017: PloS One
https://www.readbyqxmd.com/read/28805537/high-prevalence-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-wild-fish-from-the-mediterranean-sea-in-algeria
#5
Soumia Brahmi, Abdelaziz Touati, Catherine Dunyach-Remy, Albert Sotto, Alix Pantel, Jean-Philippe Lavigne
AIM: We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among wild fish from the coast of Bejaia (Algeria) in the Mediterranean Sea. RESULTS: From March 2012 to August 2013, gut and gill samples of wild fish were screened for the presence of ESBL-producing Enterobacteriaceae. Strains were characterized with regard to antibiotic resistance, β-lactamase content, plasmid-mediated quinolone resistance, aminoglycoside resistance genes, and clonality (repetitive sequence-based polymerase chain reaction profiles and multilocus sequence typing)...
August 14, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28804662/fungal-bezoar-a-rare-cause-of-ureteral-obstruction
#6
Nabil Zeineddine, Wissam Mansour, Sandy El Bitar, Marco Campitelli, Neville Mobarakai
A 52-year-old male, with diabetes mellitus and alcoholic liver disease, presented to the Emergency Room for right flank pain of 3 days' duration, associated with dysuria. Physical examination revealed right flank tenderness with fever and hypotension; laboratory findings showed acute kidney injury and large blood and leucocytes in the urine. A CT abdomen and pelvis showed hydronephrosis of the right collecting system of a horseshoe kidney with air and hyperdense debris in the renal pelvis. Patient was treated for multisensitive Proteus mirabilis emphysematous pyelonephritis, and a right nephrostomy tube was inserted...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28797330/evolving-beta-lactamase-epidemiology-in-enterobacteriaceae-from-italian-nationwide-surveillance-october-2013-kpc-carbapenemase-spreading-among-outpatients
#7
Tommaso Giani, Alberto Antonelli, Mariasofia Caltagirone, Carola Mauri, Jessica Nicchi, Fabio Arena, Elisabetta Nucleo, Silvia Bracco, Annalisa Pantosti, Francesco Luzzaro, Laura Pagani, Gian Maria Rossolini
Extended-spectrum beta-lactamases (ESBLs), AmpC-type beta-lactamases (ACBLs) and carbapenemases are among the most important resistance mechanisms in Enterobacteriaceae. This study investigated the presence of these resistance mechanisms in consecutive non-replicate isolates of Escherichia coli (n = 2,352), Klebsiella pneumoniae (n = 697), and Proteus mirabilis (n = 275) from an Italian nationwide cross-sectional survey carried out in October 2013. Overall, 15.3% of isolates were non-susceptible to extended-spectrum cephalosporins but susceptible to carbapenems (ESCR-carbaS), while 4...
August 3, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28782586/breaking-the-mirror-l-amino-acid-deaminase-a-novel-stereoselective-biocatalyst
#8
REVIEW
Gianluca Molla, Roberta Melis, Loredano Pollegioni
Enantiomerically pure amino acids are of increasing interest for the fine chemical, agrochemicals and pharmaceutical industries. During past years l-amino acids have been produced from deracemization of dl-solution employing the stereoselective flavoenzyme d-amino acid oxidase. On the other hand, the isolation of corresponding d-isomer was hampered by the scarce availability of a suitable l-amino acid oxidase activity. On this side, l-amino acid deaminase (LAAD), only present in the Proteus bacteria, represents a suitable alternative...
August 3, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28759798/bacterial-species-and-mycotoxin-contamination-associated-with-locust-bean-melon-and-their-fermented-products-in-south-western-nigeria
#9
Bamidele S Adedeji, Obinna T Ezeokoli, Chibundu N Ezekiel, Adewale O Obadina, Yinka M Somorin, Michael Sulyok, Rasheed A Adeleke, Benedikt Warth, Cyril C Nwangburuka, Adebukola M Omemu, Olusola B Oyewole, Rudolf Krska
The microbiological safety of spontaneously fermented foods is not always guaranteed due to the undefined fermenting microbial consortium and processing materials. In this study, two commonly consumed traditional condiments (iru and ogiri) and their respective raw seeds (locust bean and melon) purchased from markets in south-western Nigeria were assessed for bacterial diversity and mycotoxin contamination using 16S rRNA gene sequencing and liquid chromatography tandem mass spectrometry (LC-MS/MS), respectively...
July 25, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28757095/a-novel-type-1-2-hybrid-incc-plasmid-carrying-fifteen-antimicrobial-resistance-genes-recovered-from-proteus-mirabilis-in-china
#10
Chang-Wei Lei, Ling-Han Kong, Su-Zhen Ma, Bi-Hui Liu, Yan-Peng Chen, An-Yun Zhang, Hong-Ning Wang
IncC plasmids are of great concern as vehicles of broad-spectrum cephalosporins and carbapenems resistance genes blaCMY and blaNDM. The aim of this study was to sequence and characterize a multidrug resistance (MDR) IncC plasmid (pPm14C18) recovered from Proteus mirabilis. pPm14C18 was identified in a CMY-2-producing P. mirabilis isolate from chicken in China in 2014, and could be transferred to Escherichia coli conferring an MDR phenotype. Whole genome sequencing confirmed pPm14C18 was a novel type 1/2 hybrid IncC plasmid 165,992bp in size, containing fifteen antimicrobial resistance genes...
July 27, 2017: Plasmid
https://www.readbyqxmd.com/read/28755874/antibiofilm-potential-of-synthetic-2-amino-5-chlorobenzophenone-schiff-bases-and-its-confirmation-through-fluorescence-microscopy
#11
Arshia, Anum Khalid Khan, Khalid Mohammed Khan, Ayaz Ahmed, Muhammad Taha, Shahnaz Perveen
Antibacterial/antibiofilm potential of microwave-assisted synthetic thirty-three 2-amino-5-chloro benzophenone Schiff bases have been carried out against four bacterial strains i.e. Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus and Streptococcus mutans. Among them compounds 5, 6, 8, 9, 14, 16, 22, 24, 26, and 30-32 showed antibiofilm activities against isolates at less than 100 μg/ml concentrations. These compounds showed enhanced antibiofilm activity against S. aureus as compared to cefixime used as control...
July 26, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28749575/comparing-pairwise-additive-and-many-body-generalized-born-models-for-acid-base-calculations-and-protein-design
#12
Francesco Villa, David Mignon, Savvas Polydorides, Thomas Simonson
Generalized Born (GB) solvent models are common in acid/base calculations and protein design. With GB, the interaction between a pair of solute atoms depends on the shape of the protein/solvent boundary and, therefore, the positions of all solute atoms, so that GB is a many-body potential. For compute-intensive applications, the model is often simplified further, by introducing a mean, native-like protein/solvent boundary, which removes the many-body property. We investigate a method for both acid/base calculations and protein design that uses Monte Carlo simulations in which side chains can explore rotamers, bind/release protons, or mutate...
July 27, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28748516/colorimetric-sensing-of-toxic-metal-and-antibacterial-studies-by-using-bioextract-synthesized-silver-nanoparticles
#13
A Kalam, A G Al-Sehemi, S Alrumman, G Du, M Pannipara, Mohammed Assiri, Hussain Almalki, Mahmoud F Moustafa
Here, we report the simple and cost effective colorimetric technique for the determination of toxic metals (Hg(2+)) in aqueous sample by using bioextract silver nanoparticles (AgNPs). The indigenous AgNPs were synthesised by green and ecologically friendly style using extract of fig (Ficus carica) leaf. The synthesized AgNPs were confirmed by UV-vis spectroscopy, FT-IR spectroscopy, and scanning electron microscopy methods. The synthesis of AgNPs was observed by its colour changing from light yellow to dark brownish...
July 26, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28748050/assessment-of-facultative-anaerobes-from-the-root-canals-of-deciduous-molars-an-in-vivo-study
#14
Manisha Chandwani, Shweta Chandak
Background. The current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars. Methods. The present research enrolled 60 children in the 6‒9-year age group based on clinical and radiographic findings. Under aseptic conditions, access cavities were prepared followed by collection of samples from infected root canals with the help of sterile paper points. The samples thus obtained were subjected for microbial assay. Results. It was found that Enterococcus faecalis was isolated in 30% of cases, Escherichia coli in 28...
2017: Journal of Dental Research, Dental Clinics, Dental Prospects
https://www.readbyqxmd.com/read/28747972/antimicrobial-property-of-lemongrass-cymbopogon-citratus-oil-against-pathogenic-bacteria-isolated-from-pet-turtles
#15
B C J De Silva, Won-Gi Jung, Sabrina Hossain, S H M P Wimalasena, H N K S Pathirana, Gang-Joon Heo
The usage of essential oils as antimicrobial agents is gaining attention. Besides, pet turtles were known to harbor a range of pathogenic bacteria while the turtle keeping is a growing trend worldwide.The current study examined the antimicrobial activity of lemon grass oil (LGO) against seven species of Gram negative bacteria namely; Aeromonas hydrophila, A. caviae, Citrobacter freundii, Salmonella enterica, Edwardsiella tarda, Pseudomonas aeruginosa, and Proteus mirabilis isolated from three popular species of pet turtles...
June 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/28743658/high-level-expression-and-characterization-of-solvent-tolerant-lipase
#16
Yu Wang, Dan Luo, Yunshi Zhao, Shufang Tian, Wenpeng Deng, Chunhua Li, Lixin Ma
In this study, the coding sequence of the lipase from Proteus sp. SW1 was optimized via codon optimization and subjected to expression in Pichia pastoris GS115. The maximum enzyme yield was 387 mg/L in the supernatants of the shake-flask culture. The purified recombinant lipase exhibited a specific activity of 130 U/mg toward p-nitrophenyl Laurate. Its optimum pH and temperature were 8.0 and 40°C, respectively. It was highly stable and even activated in water-miscible solvents, showing over 102% residual activity after 24 h incubation in ethanol, acetone, isopropanol and acetonitrile...
July 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28725623/isolation-and-characterization-of-antimicrobial-compound-from-the-stem-bark-of-the-traditionally-used-medicinal-plant-adenium-obesum
#17
Mohammed Sohail Akhtar, Mohammad Amzad Hossain, Sadri Abdullah Said
BACKGROUND: Medicinal plants constitute a natural reservoir for medicines worldwide. They serve mainstream therapeutics and are central in folklore medicine. In case of Adenium obesum (Lav, Apocynaceae), indigenous people of Oman use it for the treatment of venereal diseases, wounds, skin diseases, headaches, muscle pain as well as joint pain; yet, the active ingredients have not been classified. To screened the antioxidant and antimicrobial activities of an identified compound that we isolated from the highest active chloroform extract...
July 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28725151/chemical-constituents-of-helichrysum-italicum-roth-g-don-essential-oil-and-their-antimicrobial-activity-against-gram-positive-and-gram-negative-bacteria-filamentous-fungi-and-candida-albicans
#18
Bouzid Djihane, Nouioua Wafa, Soltani Elkhamssa, De Haro Juan Pedro, Angeles Esteban Maria, Zerroug Mohamed Mihoub
The aerial parts of Helichrysum italicum (Roth) G. Don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. The essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). The most oxygenated sesquiterpene compounds were α-Cedrene (13...
July 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28722010/a-shape-shifting-redox-foldase-contributes-to-proteus-mirabilis-copper-resistance
#19
Emily J Furlong, Alvin W Lo, Fabian Kurth, Lakshmanane Premkumar, Makrina Totsika, Maud E S Achard, Maria A Halili, Begoña Heras, Andrew E Whitten, Hassanul G Choudhury, Mark A Schembri, Jennifer L Martin
Copper resistance is a key virulence trait of the uropathogen Proteus mirabilis. Here we show that P. mirabilis ScsC (PmScsC) contributes to this defence mechanism by enabling swarming in the presence of copper. We also demonstrate that PmScsC is a thioredoxin-like disulfide isomerase but, unlike other characterized proteins in this family, it is trimeric. PmScsC trimerization and its active site cysteine are required for wild-type swarming activity in the presence of copper. Moreover, PmScsC exhibits unprecedented motion as a consequence of a shape-shifting motif linking the catalytic and trimerization domains...
July 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28712201/case-of-xanthogranulomatous-oophoritis
#20
Bushra Khan, Aliya Begum Aziz, Rashida Ahmed
Xanthogranulomatous inflammation is characterized by destruction of the tissues of the organ involved and replacement by chronic inflammatory cells such as lymphocytes, plasma cells, occasional neutrophils with or without multinucleated or Touton giant cells. Exact aetiology is not known but the theory of infection with organisms like Proteus, E coli, and Bacteroides fragilis is most popular. Xanthogranulomatous inflammation of the female genital tract is not common and usually involves the endometrium; however, xanthogranulomatous inflammation of the ovaries is a rare entity...
January 2017: Journal of Ayub Medical College, Abbottabad: JAMC
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