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https://www.readbyqxmd.com/read/28337489/antibiotic-resistance-profiling-and-microbiota-of-the-upper-respiratory-tract-of-apparently-healthy-dogs-in-ibadan-south-west-nigeria
#1
Oluwafemi Babatunde Daodu, Elizabeth Adesola Amosun, Daniel Oladimeji Oluwayelu
BACKGROUND: Rearing of dogs and other pets has become increasingly popular in modern society. Bacterial flora resides within the nasal and oral cavities of dogs and when chanced, can be pathogenic. Certain similarities between humans and dogs portends dangerous behavioral habits that could lead to zoonotic disease transmission. This study was aimed at isolation, identification and antibiotic profiling of bacteria from nasal swabs of apparently healthy dogs. The zoonotic risk was also considered...
2017: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28330748/control-of-biofilm-forming-clinically-important-bacteria-by-green-synthesized-zno-nanoparticles-and-its-ecotoxicity-on-ceriodaphnia-cornuta
#2
Sekar Vijayakumar, Balasubramanian Malaikozhundan, Sathappan Shanthi, Baskaralingam Vaseeharan, Nooruddin Thajuddin
Zinc oxide nanoparticles were synthesized using the aqueous leaf extracts of Plectranthus barbatus (Plb-ZnO NPs) and characterized by UV-visible spectroscopy, XRD, FTIR. SEM and EDS and UV-visible spectra recorded the absorbance peak of Plb-ZnO NPs at 343 nm. SEM analyses showed the spherical shape of Plb-ZnO NPs with the particle size between 30 and 60 nm. Plb-ZnO NPs exhibited antibacterial and antibiofilm activity against Gram positive Bacillus subtilis at all tested concentrations. In contrast, Plb-ZnO NPs showed antibacterial and antibiofilm activity against Gram negative Vibrio parahaemolyticus and Proteus vulgaris at 100 μg/ml...
March 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28330466/the-nasopharyngeal-microbiota-of-beef-cattle-before-and-after-transport-to-a-feedlot
#3
Devin B Holman, Edouard Timsit, Samat Amat, D Wade Abbott, Andre G Buret, Trevor W Alexander
BACKGROUND: The nasopharyngeal (NP) microbiota plays an important role in bovine health, comprising a rich and diverse microbial community. The nasopharynx is also the niche for potentially pathogenic agents which are associated with bovine respiratory disease (BRD), a serious and costly illness in feedlot cattle. We used 14 beef heifers from a closed and disease-free herd to assess the dynamics of the NP microbiota of cattle that are transported to a feedlot. Cattle were sampled prior to transport to the feedlot (day 0) and at days 2, 7, and 14...
March 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28330285/screening-production-optimization-and-characterization-of-%C3%AE-glucosidase-using-microbes-from-shellfish-waste
#4
Samhita Mahapatra, A S Vickram, T B Sridharan, R Parameswari, M Ramesh Pathy
An extracellular β-glucosidase was isolated from Proteus mirabilis VIT117 found to be growing on prawn shells. The enzyme production was found to be enhanced (14.58 U/ml) when the culture was maintained at pH 9 and provided with sorbitol as carbon source, yeast extract as nitrogen source and incubated at 37 °C for approximately 72 h. Statistical methods like Plackett-Burman and RSM were also applied here to study the effects of different combinations of growth parameters for the bacteria, where the most significant parameters were found to be inoculum size, pH, yeast extract, incubation time and sorbitol...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330265/isolation-and-characterization-of-plant-synergistic-bacteria-capable-of-degrading-xenobiotics-from-oil-spillage-sites
#5
Indranil De, Sarika Gupta
Oil spillage sites primarily contain various types of hydrocarbons, such as linear chain, polycyclic, and aromatic compounds, posing several detrimental effects on plants. Results from our previous study showed an alteration of various metabolomic parameters, indirectly resulting in an observable decline of growth in the mung seedlings upon incubation with phenol, toluene, xylene, and hexane. This study evaluates the role of these compounds upon plant growth and focusses to mitigate the effect of the same, using some isolated plant synergistic bacteria...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330133/bioactive-metabolites-produced-by-streptomyces-cheonanensis-vuk-a-from-coringa-mangrove-sediments-isolation-structure-elucidation-and-bioactivity
#6
Ushakiranmayi Mangamuri, Vijayalakshmi Muvva, Sudhakar Poda, Krishna Naragani, Rajesh Kumar Munaganti, Bhujangarao Chitturi, Venkateswarlu Yenamandra
The strain VUK-A was isolated from a sediment sample of the Coringa mangrove ecosystem was identified as Streptomyces cheonanensis based on morphological, physiological, biochemical and molecular properties. Chemical investigation of the secondary metabolites of the strain Streptomyces cheonanensis VUK-A has led to the segregation of two bioactive compounds, namely 2-Methyl butyl propyl phthalate (1) and Diethyl phthalate (2) using column chromatography. The chemical structure of the active compounds was established on the basis of spectroscopic analysis, including (1)H NMR and (13)C NMR spectroscopies, FTIR and EIMS...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330100/molecular-characterization-and-amplified-ribosomal-dna-restriction-analysis-of-entomopathogenic-bacteria-associated-with-rhabditis-oscheius-spp
#7
Balakrishnan Geetha Sangeetha, Cheruvandasseri Arumughan Jayaprakas, Jinachandrannair Vijayakumari Siji, Moochattil Rajitha, Basheerkutty Shyni, Chellappan Mohandas
Bacterial strains associated with entomopathogenic nematodes (EPNs) Rhabditis (Oscheius) spp. were isolated from infected cadavers of Galleria mellonella. The obtained 18 isolates were subdivided into nine phylogenetically different genera based on comparative sequence analysis of their 16S rRNA genes. The isolates were affiliated to three different class namely γ-proteobacteria (Enterobacter, Proteus, Providencia, Pseudomonas, Stenotrophomonas), β-proteobacteria (Alcaligenes) and Bacilli (Bacillus, Enterococcus, Lysinibacillus)...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330077/diversity-and-antagonistic-potential-of-marine-microbes-collected-from-south-west-coast-of-india
#8
S Sinimol, A R Sarika, A Jayakumaran Nair
The diversity of some of the culturable microorganisms associated with marine flora and fauna collected off Vizhinjam and Mulloor coast of South India was evaluated and their bioactive production potential determined. From a total of 24 bacteria, 4 actinomycetes and 8 fungi isolated from diverse marine sources, five bacterial species-BLM3, BSP2, BCS1, BCS4 and BMA6 showed inhibitory activity against at least one of the tested pathogens viz., Klebsiella pneumonia KU1, Pseudomonas aeruginosa VL3, Salmonella enterica typhimurium MTCC 98, Escherichia coli MTCC 40, Micrococcus luteus MTCC 105, Staphylococcus simulans MTCC 3610, Proteus vulgaris MTCC 426, Vibrio fluvialis, Vibrio sp...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28304213/characterization-of-the-genetic-environment-of-the-blaveb-4-gene-associated-with-a-transposable-region-in-a-proteus-mirabilis-clinical-isolate
#9
Paula Espinal, Elisenda Miró, Laia Ramoneda, Manel Flores, Alba Rivera, Pere Coll, Ferran Navarro
Proteus mirabilis is the second most common cause of urinary tract infections and is also an important cause of nosocomial infections. TEM-type and CTX-M-type extended-spectrum β-lactamases (ESBLs) are the most widely distributed in this bacterial species, but minor ESBLs such as the VEB-type have also been identified. The aim of this study was to analyze the genetic environment of the blaVEB-4 gene found in a P. mirabilis clinical isolate recovered in Spain. P. mirabilis N2231 showed resistance to penicillins, cephalosporins, and aminoglycosides, remaining susceptible to imipenem, cefoxitin, β-lactamases inhibitors, and quinolones...
March 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28299769/the-effect-of-urinary-catheters-on-microbial-biofilms-and-catheter-associated-urinary-tract-infections
#10
Sahra Kirmusaoglu, Seyhun Yurdugül, Ahmet Metin, Suphi Vehid
PURPOSE: The aims of this study were to determine relationship between biofilm producer microorganisms attached to urinary catheters (UCs) and urinary catheter-associated urinary tract infections (CAUTIs), to determine the rate of CAUTI development and the relationship between CAUTI and catheterization period in catheterized patients. MATERIALS AND METHODS: Urinary catheters from 143 inpatients who were hospitalized in Abant Izzet Baysal University Hospital Urinary Service, and urine samples of these patients before and after catheterization of urinarycatheter were collected...
March 16, 2017: Urology Journal
https://www.readbyqxmd.com/read/28298454/in-vitro-activity-of-imipenem-against-carbapenemase-positive-enterobacteriaceae-smart-global-surveillance-program-2008-2014
#11
James A Karlowsky, Sibylle H Lob, Krystyna M Kazmierczak, Robert E Badal, Katherine Young, Mary R Motyl, Daniel F Sahm
The Study for Monitoring Antimicrobial Resistance Trends (SMART) global surveillance program collected 103,960 isolates of Enterobacteriaceae from 2008 to 2014. From this isolate collection all ertapenem-non-susceptible isolates (MIC, ≥1 μg/ml; n = 3,428) and 9,371 isolates of Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis with an ertapenem-susceptible extended-spectrum β-lactamase (ESBL)-positive phenotype were assessed for the presence of common carbapenemase genes using the Check-MDR CT101 microarray (Check-Points, Wageningen, the Netherlands) and published multiplex PCR assays...
March 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28291749/proteolysis-of-truncated-hemolysin-a-yields-a-stable-dimerization-interface
#12
Walter R P Novak, Basudeb Bhattacharyya, Daniel P Grilley, Todd M Weaver
Wild-type and variant forms of HpmA265 (truncated hemolysin A) from Proteus mirabilis reveal a right-handed, parallel β-helix capped and flanked by segments of antiparallel β-strands. The low-salt crystal structures form a dimeric structure via the implementation of on-edge main-chain hydrogen bonds donated by residues 243-263 of adjacent monomers. Surprisingly, in the high-salt structures of two variants, Y134A and Q125A-Y134A, a new dimeric interface is formed via main-chain hydrogen bonds donated by residues 203-215 of adjacent monomers, and a previously unobserved tetramer is formed...
March 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28285420/fosfomycin-and-comparator-activity-against-select-enterobacteriaceae-pseudomonas-and-enterococcus-urinary-tract-infection-isolates-from-the-united-states-in-2012
#13
Tiffany R Keepers, Marcela Gomez, Chris Celeri, Kevin M Krause, Donald Biek, Ian Critchley
INTRODUCTION: Fosfomycin is a broad-spectrum cell wall active agent that inhibits the MurA enzyme involved in peptidoglycan synthesis and is FDA-approved for treatment of uncomplicated urinary tract infections (UTIs) caused by Escherichia coli and Enterococcus faecalis in women. Data regarding the susceptibility of recent UTI isolates to fosfomycin are limited. METHODS: This study compared the fosfomycin susceptibility of 658 US UTI isolates with susceptibility to ciprofloxacin, levofloxacin, nitrofurantoin, and trimethoprim/sulfamethoxazole (SXT)...
March 11, 2017: Infectious Diseases and Therapy
https://www.readbyqxmd.com/read/28270849/prevalence-of-extended-spectrum-beta-lactamases-producing-microorganisms-in-patients-admitted-at-krrh-southwestern-uganda
#14
Baguma Andrew, Atek Kagirita, Joel Bazira
The emergence of extended-spectrum beta-lactamase- (ESBL-) producing pathogenic bacteria at Kabale Regional Referral Hospital (KRRH), located in southwestern Uganda, is of great concern: a phenomenon that worries clinicians and other healthcare workers due to the serious threat they pose to patients. This current study aimed at determining the phenotypic detection of ESBL-producing strains of E. coli, Klebsiella sp., and Proteus sp. isolated from clinical specimens and their prevalence in patients admitted at KRRH...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28264656/antimicrobial-susceptibilities-of-aerobic-and-facultative-gram-negative-bacilli-isolated-from-chinese-patients-with-urinary-tract-infections-between-2010-and-2014
#15
Qiwen Yang, Hui Zhang, Yao Wang, Zhipeng Xu, Ge Zhang, Xinxin Chen, Yingchun Xu, Bin Cao, Haishen Kong, Yuxing Ni, Yunsong Yu, Ziyong Sun, Bijie Hu, Wenxiang Huang, Yong Wang, Anhua Wu, Xianju Feng, Kang Liao, Yanping Luo, Zhidong Hu, Yunzhuo Chu, Juan Lu, Jianrong Su, Bingdong Gui, Qiong Duan, Shufang Zhang, Haifeng Shao, Robert E Badal
BACKGROUND: The objective of this study was to investigate the distribution and susceptibility of aerobic and facultative Gram-negative bacilli isolated from Chinese patients with UTIs collected within 48 h (community acquired, CA) or after 48 h (hospital acquired, HA) of hospital admission. METHODS: From 2010 to 2014, the minimum inhibitory concentrations (MICs) of 12 antibiotics for 4,332 aerobic and facultative Gram-negative bacilli, sampled in 21 hospitals in 16 cities, were determined by the broth microdilution method...
March 6, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28257904/in-vitro-activity-of-ceftaroline-and-comparator-agents-against-gram-positive-and-gram-negative-clinical-isolates-from-cancer-patients
#16
Kenneth V I Rolston, Mohamed A Jamal, Lior Nesher, Samuel A Shelburne, Issam Raad, Randall A Prince
Bacterial infections are common in cancer patients. Ceftaroline (CFT) is a broad-spectrum cephalosporin with activity against most Gram-positive organisms (GPOs) and many Gram-negative organisms. In this study, the in vitro activity of CFT was compared with vancomycin (VAN), daptomycin (DAP), linezolid (LZD), trimethoprim/sulphamethoxazole (SXT) and tigecycline (TIG) against bacteria (predominantly blood culture isolates) isolated from cancer patients in 2014 and 2015. CFT was active against methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant S...
February 28, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28247737/-experimental-modeling-of-acute-pyelonephritis
#17
M I Kogan, Y U L Naboka, I A Gudima, S K Bedjanyan
Acute pyelonephritis (AP) predominates among inflammatory infection kidney affections. In accordance to international classifications, AP is an upper urinary tract infection, subdivided into non-complicated (non-obstructive) and complicated (obstructive) forms. The clinical significance of AP presently is defined by the conditions frequent occurrence and its progression into chronic kidney disease. For a limited number of pathogens the involvement in AP is considered proven: representatives of the Enterobacteriaceae family (E...
August 2016: Urologii︠a︡
https://www.readbyqxmd.com/read/28245252/very-high-prevalence-of-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-in-bacteriemic-patients-hospitalized-in-teaching-hospitals-in-bamako-mali
#18
Samba Adama Sangare, Emilie Rondinaud, Naouale Maataoui, Almoustapha Issiaka Maiga, Ibrehima Guindo, Aminata Maiga, Namory Camara, Oumar Agaly Dicko, Sounkalo Dao, Souleymane Diallo, Flabou Bougoudogo, Antoine Andremont, Ibrahim Izetiegouma Maiga, Laurence Armand-Lefevre
The worldwide dissemination of extended-spectrum beta-lactamase producing Enterobacteriaceae, (ESBL-E) and their subset producing carbapenemases (CPE), is alarming. Limited data on the prevalence of such strains in infections from patients from Sub-Saharan Africa are currently available. We determined, here, the prevalence of ESBL-E/CPE in bacteriemic patients in two teaching hospitals from Bamako (Mali), which are at the top of the health care pyramid in the country. During one year, all Enterobacteriaceae isolated from bloodstream infections (E-BSI), were collected from patients hospitalized at the Point G University Teaching Hospital and the pediatric units of Gabriel Touré University Teaching Hospital...
2017: PloS One
https://www.readbyqxmd.com/read/28241439/synthesis-characterization-and-antibacterial-studies-of-n-benzothiazol-2-yl-4-chlorobenzenesulphonamide-and-its-neodymium-iii-and-thallium-iii-complexes
#19
Lawrence Nnamdi Obasi, Uchechukwu Susan Oruma, Ibrahim Abdulrazak Al-Swaidan, Ponnadurai Ramasami, Chigozie Julius Ezeorah, Alfred Ezinna Ochonogor
N-(Benzothiazol-2-yl)-4-chlorobenzenesulphonamide (NBTCS) was synthesized by condensation reaction of 4-chlorobenzenesulphonyl chloride and 2-aminobenzothiazole in acetone under reflux. Neodymium(III) and thallium(III) complexes of the ligand were also synthesized. Both ligand and metal complexes were characterized using UV-Vis, IR, ¹H- and (13)C-NMR spectroscopies, elemental analysis and molar conductance measurement. IR studies revealed that the ligand is tridentate and coordinates to the metal ions through nitrogen and oxygen atoms of the sulphonamide group and nitrogen atom attached to benzothiazole ring...
February 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28239963/characterization-of-complex-heterogeneous-lipid-a-samples-using-hplc-ms-ms-technique-ii-structural-elucidation-of-non-phosphorylated-lipid-a-by-negative-ion-mode-tandem-mass-spectrometry
#20
Viktor Sándor, Anikó Kilár, Ferenc Kilár, Béla Kocsis, Ágnes Dörnyei
Non-phosphorylated lipid A species confer reduced inflammatory potential for the bacteria. Knowledge on their chemical structure and presence in bacterial pathogens may contribute to the understanding of bacterial resistance and activation of the host innate immune system. In this study, we report the fragmentation pathways of negatively charged, non-phosphorylated lipid A species under low-energy collision-induced dissociation conditions of an electrospray ionization quadrupole time-of-flight instrument. Charge-promoted consecutive and competitive eliminations of the acyl chains and cross-ring cleavages of the sugar residues were observed...
August 2016: Journal of Mass Spectrometry: JMS
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