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Hongge Jia, Yunzen Zhang, Vladimir Orbovic, Jin Xu, Frank White, Jeffrey Jones, Nian Wang
Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility, and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance...
December 9, 2016: Plant Biotechnology Journal
Victoria B Stockman, Lila Ghamsari, Gorka Lasso, Barry Honig, Sagi D Shapira, Harris H Wang
Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPR-Cas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible with next-generation sequencing preparation and quantification...
2016: PloS One
T Peter Kingham, Hoang C B Nguyen, Jian Zheng, Ioannis T Konstantinidis, Eran Sadot, Jinru Shia, Deborah Kuk, Steven Zhang, Leonard Saltz, Michael I D'Angelica, William R Jarnagin, Hani Goodarzi, Sohail F Tavazoie
BACKGROUND: Resection of colorectal liver metastasis (CRLM) can be curative. Predicting which patients may benefit from resection, however, remains challenging. Some microRNAs (miRNAs) become deregulated in cancers and contribute to cancer progression. We hypothesized that miRNA expression can serve as a prognostic marker of survival after CRLM resection. RESULTS: MiR-203 was significantly overexpressed in tumors of short-term survivors compared to long-term survivors...
December 7, 2016: Oncotarget
Deyang Chen, Zuhuang Chen, Qian He, James D Clarkson, Claudy R Serrao, Ajay K Yadav, Mark E Nowakowski, Zhen Fan, Long You, Xingsen Gao, Dechang Zeng, Lang Chen, Albina Y Borisevich, Sayeef Salahuddin, Jun-Ming Liu, Jeffrey Bokor
A wealth of fascinating phenomena have been discovered at the BiFeO3 domain walls, examples such as domain wall conductivity, photovoltaic effects, and magnetoelectric coupling. Thus, the ability to precisely control the domain structures and accurately study their switching behaviors is critical to realize the next generation of novel devices based on domain wall functionalities. In this work, the introduction of a dielectric layer leads to the tunability of the depolarization field both in the multilayers and superlattices, which provides a novel approach to control the domain patterns of BiFeO3 films...
December 9, 2016: Nano Letters
Timothée Menais, Stefano Mossa, Arnaud Buhot
Polymer translocation is a promising strategy for the next-generation DNA sequencing technologies. The use of biological and synthetic nano-pores, however, still suffers from serious drawbacks. In particular, the width of the membrane layer can accommodate several bases at the same time, making difficult accurate sequencing applications. More recently, the use of graphene membranes has paved the way to new sequencing capabilities, with the possibility to measure transverse currents, among other advances. The reduced thickness of these new membranes poses new questions on the effect of deformability and vibrations of the membrane on the translocation process, two features which are not taken into account in the well established theoretical frameworks...
December 9, 2016: Scientific Reports
Kateryna Bazaka, Ryan Destefani, Mohan V Jacob
This article presents low-temperature, one-step dry synthesis of optically transparent thermally-stable, biocompatible cis-β-ocimene-based thin films for applications as interlayer dielectric and encapsulating layer for flexible electronic devices, e.g. OLEDs. Morphological analysis of thin films shows uniform, very smooth (Rq < 1 nm) and defect-free moderately hydrophilic surfaces. The films are optically transparent, with a refractive index of ~1.58 at 600 nm, an optical band gap of ~2.85 eV, and dielectric constant of 3...
December 9, 2016: Scientific Reports
Inga Möller, Rajmohan Murali, Hansgeorg Müller, Thomas Wiesner, Louise A Jackett, Simone L Scholz, Ioana Cosgarea, Johannes Ap van de Nes, Antje Sucker, Uwe Hillen, Bastian Schilling, Annette Paschen, Heinz Kutzner, Arno Rütten, Martin Böckers, Richard A Scolyer, Dirk Schadendorf, Klaus G Griewank
Blue nevi are common melanocytic tumors arising in the dermal layer of the skin. Similar to uveal melanomas, blue nevi frequently harbor GNAQ and GNA11 mutations. Recently, recurrent CYSLTR2 and PLCB4 mutations were identified in uveal melanomas not harboring GNAQ or GNA11 mutations. All four genes (GNAQ, GNA11, CYSLTR2, and PLCB4) code for proteins involved in the same signaling pathway, which is activated by mutations in these genes. Given the related functional consequences of these mutations and the known genetic similarities between uveal melanoma and blue nevi, we analyzed a cohort of blue nevi to investigate whether CYSLTR2 and PLCB4 mutations occur in tumors lacking GNAQ or GNA11 mutations (as in uveal melanoma)...
December 9, 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
Christoph K Naber, Stylianos A Pyxaras, Hüseyin Ince, Peter Frambach, Antonio Colombo, Christian Butter, Fernando Gatto, Ulrich Hink, Georg Nickenig, Giuseppe Bruschi, Guus Brueren, Didier Tchétché, Peter Den Heijer, Wolfgang Schillinger, Smita Scholtz, Jan Van der Heyden, Thierry Lefèvre, Martine Gilard, Karl-Heinz Kuck, Joachim Schofer, Dimitar Divchev, Helmut Baumgartner, Federico Asch, Daniel Wagner, Azeem Latib, Federico De Marco, Stephan Kische
AIMS: Our aim was to assess the clinical outcomes of the Direct Flow Medical Transcatheter Aortic Valve System (DFM-TAVS), when used in routine clinical practice. METHODS AND RESULTS: This is a prospective, open-label, multicentre, post-market registry of patients treated with DFM-TAVS according to approved commercial indications. Echocardiographic and angiographic data were evaluated by an independent core laboratory and adverse events were adjudicated and classified according to VARC-2 criteria by an independent clinical events committee...
December 10, 2016: EuroIntervention
Birte Martin-Bertelsen, Anan Yaghmur, Henrik Franzyk, Sarah Justesen, Jacob J K Kirkensgaard, Camilla Foged
Synthetic analogues of the cell-wall lipid monomycoloyl glycerol (MMG) are promising as next-generation vaccine adjuvants. In the present study, the thermotropic phase behavior of an array of synthetic MMG analogues was examined by using simultaneous small- and wide-angle X-ray scattering under excess water conditions. The MMG analogues differed in the alkyl chain lengths and in the stereochemistry of the polar glycerol headgroup or of the lipid tails (native-like versus alternative compounds). All MMG analogues formed poorly hydrated lamellar phases at low temperatures and inverse hexagonal (H2) phases at higher temperatures prior to melting...
December 6, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Dabum Kim, Jeonghun Kim, Youngsang Ko, Kyubin Shim, Jung Ho Kim, Jungmok You
We developed a novel strategy for fabricating poly(3,4-ethylenedioxythiophene) (PEDOT) patterns on various substrates, including hydrogels, via sequential solution procedure without multistep chemical etching or lift-off processes. First, PEDOT nanothin films were prepared on a glass substrate by solution phase monomer casting and oxidative polymerization. As a second step, after UV-induced poly(ethylene glycol) (PEG) photolithography at the PEDOT/PEG interface through a photomask, the hydrogel was peeled away from the PEDOT-coated glass substrate to detach the UV-exposed PEDOT region, which left the UV nonexposed PEDOT region intact on the glass substrate, resulting in PEDOT patterns...
December 7, 2016: ACS Applied Materials & Interfaces
Fatemeh Sadat Minaye Hashemi, Bradlee R Birchansky, Stacey F Bent
Area selective atomic layer deposition has the potential to significantly improve current fabrication approaches by introducing a bottom-up process in which robust and conformal thin films are selectively deposited onto patterned substrates. In this paper, we demonstrate selective deposition of dielectrics on metal/dielectric patterns by protecting metal surfaces using alkanethiol blocking layers. We examine alkanethiol self-assembled monolayers (SAMs) with two different chain lengths deposited both in vapor and in solution and show that in both systems, thiols have the ability to block surfaces against dielectric deposition...
December 7, 2016: ACS Applied Materials & Interfaces
Mani Ulaganathan, Reinack Varghese Hansen, Nateisha Drayton, Hardik Hingorani, R Govindan Kutty, Hrishikesh Joshi, Sivaramapanicker Sreejith, Zheng Liu, Jinglei Yang, Yanli Zhao
Linear two-dimensional materials have recently attracted an intense interest for supercapacitors because of their potential uses as electrodes in next-generation wearable electronics. However, enhancing the electrochemical properties of these materials without complicated structural modifications remains a challenge. Herein, we present the preparation of a hybrid electrode system via polydiacetylene (PDA) cloaking on the surface of aligned multiwall carbon nanotubes (MWCNTs) through self-assembly based in situ photopolymerization...
December 7, 2016: ACS Applied Materials & Interfaces
Lilu Liu, Xingguo Qi, Qiang Ma, Xiaohui Rong, Yong-Sheng Hu, Zhibin Zhou, Hong Li, Xuejie Huang, Liquan Chen
A non-sintered method with toothpaste electrode for improving electrode ionic conductivity and reducing interface impedance is introduced in solid-state rechargeable batteries. At 70 °C, this novel solid-state battery can deliver a capacity of 80 mAh g(-1) in a voltage range of 2.5-3.8 V at 0.1C rate using layered oxide Na0.66Ni0.33Mn0.67O2, Na-β″-Al2O3 and sodium metal as cathode, electrolyte and anode, respectively. Moreover, the battery shows a superior stability and high reversibility, with a capacity retention of 90% after 10 000 cycles at 6C rate and a capacity of 79 mAh g(-1) is recovered when the current rate is returned to 0...
December 7, 2016: ACS Applied Materials & Interfaces
Zhiwen Jin, Dan He, Qing Zhou, Peng Mao, Liming Ding, Jizheng Wang
Organic-based photodetectors (PDs) have great potential applications in next-generation portable, low-cost, large-area displays and optical communications. However, for practical applications, they are facing big challenges due to their instabilities in ambient environments, especially under high temperatures. Robust materials and device architectures are highly demanded to overcome the problem. In this report, we employed a donor conjugated polymer PThTPTI and realized thin-film PDs which can stably operate in ambient air under temperatures as high as 300 °C...
December 7, 2016: ACS Applied Materials & Interfaces
A M Scaparro, V Miseikis, C Coletti, A Notargiacomo, M Pea, M De Seta, L Di Gaspare
Germanium is emerging as the substrate of choice for the growth of graphene in CMOS-compatible processes. For future application in next generation devices the accurate control over the properties of high-quality graphene synthesized on Ge surfaces, such as number of layers and domain size, is of paramount importance. Here we investigate the role of the process gas flows on the CVD growth of graphene on Ge(100). The quality and morphology of the deposited material is assessed by using μ-Raman spectroscopy, X-ray photoemission spectroscopy, scanning electron microscopy, and atomic force microscopy...
December 7, 2016: ACS Applied Materials & Interfaces
Yunsheng Fang, Ke Ding, Zhicong Wu, Hongting Chen, Wenbo Li, Sheng Zhao, Yanli Zhang, Lei Wang, Jun Zhou, Bin Hu
Replacing rigid metal oxides with flexible alternatives as a next-generation transparent conductor is important for flexible optoelectronic devices. Recently, nanowire networks have emerged as a new type of transparent conductor and have attracted wide attention because of their all-solution-based process manufacturing and excellent flexibility. However, the intrinsic percolation characteristics of the network determine that its fine pattern behavior is very different from that of continuous films, which is a critical issue for their practical application in high-resolution devices...
November 22, 2016: ACS Nano
Kyle E Giesler, Dennis C Liotta
The pharmacokinetic properties of tenofovir (TFV) and other charged nucleoside analogues are dramatically improved upon conjugation to a lipid prodrug. We previously prepared reduction-sensitive lipid conjugates of TFV that demonstrate superior antiviral activity compared to other lipid conjugates including the clinically approved formulation, tenofovir disoproxil fumarate (TDF). In continuation of that work, we have synthesized next-generation conjugates with reduced cytotoxicity that retain potent antiviral activity against HIV-1 and HBV with a therapeutic index >100000 for our most potent conjugate...
November 23, 2016: Journal of Medicinal Chemistry
Muhammad Safdar, Tam Do Minh, Niko Kinnunen, Janne Jänis
Manganese oxide (MnO2) has recently emerged as a promising alternate material for the fabrication of self-propelled micromotors. Platinum (Pt) has been traditionally used as a catalytic material for this purpose. However, the high cost associated with Pt restricts its widespread use toward practical applications where large amounts of material are required. MnO2 exists in different crystalline forms (polymorphs), which govern its catalytic behavior. In spite of this, the recent reports on MnO2 based micromotors have seldom reported on the polymorphic form involved...
November 30, 2016: ACS Applied Materials & Interfaces
Ting-Feng Yi, Yan-Mei Li, Shuang-Yuan Yang, Yan-Rong Zhu, Ying Xie
Layered Li-rich, Co-free, and Mn-based cathode material, Li1.17Ni0.25-xMn0.58MgxO2 (0 ≤ x ≤ 0.05), was successfully synthesized by a coprecipitation method. All prepared samples have typical Li-rich layered structure, and Mg has been doped in the Li1.17Ni0.25Mn0.58O2 material successfully and homogeneously. The morphology and the grain size of all material are not changed by Mg doping. All materials have a estimated size of about 200 nm with a narrow particle size distribution. The electrochemical property results show that Li1...
November 30, 2016: ACS Applied Materials & Interfaces
Michal Feingold-Zadok, David Chitayat, Karen Chong, Marie Injeyan, Patrick Shannon, Daphne Chapmann, Ron Maymon, Nir Pillar, Orit Reish
OBJECTIVE: We studied a series of patients with fetal akinesia deformation sequence (FADS)/arthrogryposis multiplex congenita (AMC), with nemaline bodies on muscle specimens, that revealed mutations in the NEB gene. METHOD: We pathologically assessed seven cases from three families, who presented with AMC/FADS. Targeted genetic analysis for Ashkenazi Jewish (AJ) mutation (in relevant patients) was followed by next generation sequencing and Multiplex Ligation-dependent Probe Amplification...
December 9, 2016: Prenatal Diagnosis
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