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https://www.readbyqxmd.com/read/29779063/development-of-sperm-vitrification-protocols-for-two-endangered-salmonid-species-the-adriatic-grayling-thymallus-thymallus-and-the-marble-trout-salmo-marmoratus
#1
Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA)...
May 19, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29776697/cinnamtannin-b-1-a-novel-antioxidant-for-sperm-in-red-deer
#2
F Sánchez-Rubio, M R Fernández-Santos, L Castro-Vázquez, O García-Álvarez, A Maroto-Morales, A J Soler, F Martínez-Pastor, J J Garde
Cinnamtannin B-1 (CNB-1) is a naturally occurring trimeric A-type proanthocyanidin contained in several plants such as cinnamon (Cinnamomum zeylanicum). It is considered to be a potent antioxidant. The protective effect of CNB-1 against oxidative stress was assessed in red deer epididymal sperm incubated at 37 °C. Cryopreserved sperm from six stags were thawed, pooled and extended to 400 × 106 sperm/ml in BGM (bovine gamete medium). After being aliquoted, the samples were supplemented with different concentrations of CNB-1 (0, 0...
May 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29773294/gastroesophageal-reflux-symptoms-and-nasal-symptoms-affect-the-severity-of-bronchitis-symptoms-in-patients-with-chronic-obstructive-pulmonary-disease
#3
Koichi Hasegawa, Susumu Sato, Kazuya Tanimura, Yoshinori Fuseya, Kiyoshi Uemasu, Yoko Hamakawa, Atsuyasu Sato, Michiaki Mishima, Shigeo Muro, Toyohiro Hirai
BACKGROUND: Cough and sputum production (symptoms of bronchitis) are common in chronic obstructive pulmonary disease (COPD). Extrapulmonary comorbidities, such as gastroesophageal reflux disease (GERD) and post-nasal drip, also cause bronchitis symptoms. The impact of extrapulmonary comorbidities on the severity of bronchitis symptoms in COPD is unknown. The aim of this study was to quantify bronchitis symptoms and assess the impact of GERD and nasal symptoms on the severity of bronchitis symptoms in COPD...
May 2018: Respiratory Investigation
https://www.readbyqxmd.com/read/29768696/the-isohydric-trap-a-proposed-feedback-between-water-shortage-stomatal-regulation-and-nutrient-acquisition-drives-differential-growth-and-survival-of-european-pines-under-climatic-dryness
#4
D Salazar-Tortosa, J Castro, P Villar-Salvador, B Viñegla, L Matías, A Michelsen, R Rubio de Casas, J I Querejeta
Climatic dryness imposes limitations on vascular plant growth by reducing stomatal conductance, thereby decreasing CO2 uptake and transpiration. Given that transpiration-driven water flow is required for nutrient uptake, climatic stress-induced nutrient deficit could be a key mechanism for decreased plant performance under prolonged drought. We propose the existence of an "isohydric trap", a dryness-induced detrimental feedback leading to nutrient deficit and stoichiometry imbalance in strict isohydric species...
May 16, 2018: Global Change Biology
https://www.readbyqxmd.com/read/29768446/inhibitory-effect-of-k-ions-and-influence-of-other-ions-and-osmolality-on-the-spermatozoa-motility-of-european-burbot-lota-lota-l
#5
Katarzyna Dziewulska, Malwina Pilarska
BACKGROUND: In fish with external fertilization, two main start-up mechanisms of the path that blocks or activates the spermatozoan motility apparatus are known. The main factor managing the path is osmolality or potassium ion. In burbot from the European and North American population, contradictory findings regarding the factors influencing the onset of spermatozoa motility were reported. The objective of the current study was to determine the effect of potassium and osmolality on the spermatozoa activation of European burbot, Lota lota (Actinopterygii, Gadiformes, Lotidae)...
2018: PloS One
https://www.readbyqxmd.com/read/29766456/prognostic-role-for-the-derived-neutrophil-to-lymphocyte-ratio-in-early-breast-cancer-a-geicam-9906-substudy
#6
A J Templeton, Á Rodríguez-Lescure, A Ruíz, E Alba, L Calvo, M Ruíz-Borrego, A Santaballa, C A Rodríguez, C Crespo, M Ramos, J M Gracia-Marco, A Lluch, I Álvarez, M I Casas, M Sánchez-Aragó, R Caballero, E Carrasco, E Amir, M Martin, A Ocaña
PURPOSE: Elevated markers of host inflammation, a hallmark of cancer, have been associated with worse outcomes in several solid tumors. Here, we explore the prognostic role of the derived neutrophil-to-lymphocyte ratio (dNLR), across different tumor subtypes, in patients with early breast cancer. PATIENTS AND METHODS: This was a retrospective analysis of 1246 patients with lymph node-positive, operable early breast cancer enrolled in the GEICAM/9906 trial, a multicenter randomized phase 3 study evaluating adjuvant chemotherapy...
May 15, 2018: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/29762343/team-approach-metastatic-disease-of-the-spine
#7
Antonia Matamalas, Claudia Valverde, Sergi Benavente, Lourdes Casas-Gomila, Cleofé Romagosa, Pilar González-Tartière, Ferran Pellisé
No abstract text is available yet for this article.
May 15, 2018: JBJS Reviews
https://www.readbyqxmd.com/read/29762169/change-in-sexual-risk-behaviour-after-six-months-of-pre-exposure-prophylaxis-use-results-from-the-amsterdam-prep-demonstration-project
#8
Elske Hoornenborg, Liza Coyer, Anna van Laarhoven, Roel Achterbergh, Henry de Vries, Maria Prins, Maarten Schim van der Loeff
OBJECTIVE: HIV pre-exposure prophylaxis (PrEP) use may lead to higher STI incidence via behavioral risk compensation. We examined changes in sexual behaviour between baseline and six months after PrEP initiation among men who have sex with men (MSM) and transgender women (TGW). DESIGN: Prospective, open-label demonstration study at a large sexually transmitted infections (STI) clinic in Amsterdam, the Netherlands. METHODS: Participants answered questions about sexual behaviour in the preceding three months, including number of anal sex partners and frequency of anal sex with and without condom by partner type and were tested for STI...
May 11, 2018: AIDS
https://www.readbyqxmd.com/read/29761888/idiopathic-sterile-pyogranuloma-in-three-domestic-cats
#9
A Giuliano, P Watson, L Owen, B Skelly, L Davison, J Dobson, F Costantino-Casas
Pyogranulomatous inflammation has been extensively described in cats, in particular in cases of feline infectious peritonitis and also associated with Mycobacteria, Actinomyces, Nocardia, Rhodococcus and fungal infections. Idiopathic sterile pyogranulomatous dermatitis has also been described. In this case series we describe the clinical presentation, histopathology and outcome of three cases of feline idiopathic sterile steroid-responsive pyogranuloma with different presentation and different locations of the lesion, but with the common feature of having a mass with no superficial skin involvement...
May 15, 2018: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29760242/cochrane-corner-text-messaging-to-improve-adherence-to-drugs-for-secondary-prevention-of-cardiovascular-disease
#10
EDITORIAL
Alma J Adler, Juan P Casas, Nicole Martin, Caroline Free, Pablo Perel
No abstract text is available yet for this article.
May 14, 2018: Heart: Official Journal of the British Cardiac Society
https://www.readbyqxmd.com/read/29759923/madelung-s-disease-in-a-patient-with-advanced-hepatocellular-carcinoma
#11
Diego Casas Deza, Jesús Gotor Delso, Vanesa Bernal Monterde
No abstract text is available yet for this article.
May 11, 2018: Gastroenterología y Hepatología
https://www.readbyqxmd.com/read/29756985/-consensus-document-about-the-nutritional-evaluation-and-management-of-eating-disorders-anorexia-nervosa-bulimia-nervosa-binge-eating-disorder-and-others-executive-abstract
#12
Carmen Gómez Candela, Samara Palma Milla, Alberto Miján-de-la-Torre, Pilar Rodríguez Ortega, Pilar Matía Martín, Viviana Loria Kohen, Rocío Campos Del Portillo, M ª Nuria Virgili Casas, Miguel Á Martínez Olmos, María Teresa Mories Álvarez, María José Castro Alija, Ángela Martín-Palmero
No abstract text is available yet for this article.
January 31, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29756838/magnetic-excitations-across-the-metal-insulator-transition-in-the-pyrochlore-iridate-eu_-2-ir_-2-o_-7
#13
Sae Hwan Chun, Bo Yuan, Diego Casa, Jungho Kim, Chang-Yong Kim, Zhaoming Tian, Yang Qiu, Satoru Nakatsuji, Young-June Kim
We report a resonant inelastic x-ray scattering study of the magnetic excitation spectrum in a highly insulating Eu_{2}Ir_{2}O_{7} single crystal that exhibits a metal-insulator transition at T_{MI}=111(7)  K. A propagating magnon mode with a 20 meV bandwidth and a 28 meV magnon gap is found in the excitation spectrum at 7 K, which is expected in the all-in-all-out magnetically ordered state. This magnetic excitation exhibits substantial softening as the temperature is raised towards T_{MI} and turns into a highly damped excitation in the paramagnetic phase...
April 27, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/29755676/genetic-heterogeneity-and-actionable-mutations-in-her2-positive-primary-breast-cancers-and-their-brain-metastases
#14
Leticia De Mattos-Arruda, Charlotte K Y Ng, Salvatore Piscuoglio, Maria Gonzalez-Cao, Raymond S Lim, Maria R De Filippo, Nicola Fusco, Anne M Schultheis, Carolina Ortiz, Santiago Viteri, Alexandra Arias, Gabriel S Macedo, Mafalda Oliveira, Patricia Gomez, Cristina Teixidó, Paolo Nuciforo, Vicente Peg, Cristina Saura, Santiago Ramon Y Cajal, Francesc Tresserra Casas, Britta Weigelt, Javier Cortes, Joan Seoane, Jorge S Reis-Filho
Brain metastases constitute a challenge in the management of patients with HER2-positive breast cancer treated with anti-HER2 systemic therapies. Here we sought to define the repertoire of mutations private to or enriched for in HER2-positive brain metastases. Massively parallel sequencing targeting all exons of 254 genes frequently mutated in breast cancers and/or related to DNA repair was used to characterize the spatial and temporal heterogeneity of HER2-positive breast cancers and their brain metastases in six patients...
April 17, 2018: Oncotarget
https://www.readbyqxmd.com/read/29754954/the-polycomb-dependent-epigenome-controls-%C3%AE-cell-dysfunction-dedifferentiation-and-diabetes
#15
Tess Tsai-Hsiu Lu, Steffen Heyne, Erez Dror, Eduard Casas, Laura Leonhardt, Thorina Boenke, Chih-Hsiang Yang, Sagar, Laura Arrigoni, Kevin Dalgaard, Raffaele Teperino, Lennart Enders, Madhan Selvaraj, Marius Ruf, Sunil J Raja, Huafeng Xie, Ulrike Boenisch, Stuart H Orkin, Francis C Lynn, Brad G Hoffman, Dominic Grün, Tanya Vavouri, Adelheid M Lempradl, J Andrew Pospisilik
To date, it remains largely unclear to what extent chromatin machinery contributes to the susceptibility and progression of complex diseases. Here, we combine deep epigenome mapping with single-cell transcriptomics to mine for evidence of chromatin dysregulation in type 2 diabetes. We find two chromatin-state signatures that track β cell dysfunction in mice and humans: ectopic activation of bivalent Polycomb-silenced domains and loss of expression at an epigenomically unique class of lineage-defining genes...
May 4, 2018: Cell Metabolism
https://www.readbyqxmd.com/read/29750270/peripheral-re-localization-of-constitutive-heterochromatin-advances-its-replication-timing-and-impairs-maintenance-of-silencing-marks
#16
Kathrin S Heinz, Corella S Casas-Delucchi, Timea Török, Dusan Cmarko, Alexander Rapp, Ivan Raska, M Cristina Cardoso
The replication of the genome is a highly organized process, both spatially and temporally. Although a lot is known on the composition of the basic replication machinery, how its activity is regulated is mostly unknown. Several chromatin properties have been proposed as regulators, but a potential role of the nuclear DNA position remains unclear. We made use of the prominent structure and well-defined heterochromatic landscape of mouse pericentric chromosome domains as a well-studied example of late replicating constitutive heterochromatin...
May 10, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29748539/sirt1-activation-with-neuroheal-is-neuroprotective-but-sirt2-inhibition-with-ak7-is-detrimental-for-disconnected-motoneurons
#17
David Romeo-Guitart, Tatiana Leiva-Rodríguez, María Espinosa-Alcantud, Núria Sima, Alejandro Vaquero, Helena Domínguez-Martín, Diego Ruano, Caty Casas
Sirtuin 1 (SIRT1) activity is neuroprotective, and we have recently demonstrated its role in the retrograde degenerative process in motoneurons (MNs) in the spinal cord of rats after peripheral nerve root avulsion (RA) injury. SIRT2 has been suggested to exert effects opposite those of SIRT1; however, its roles in neurodegeneration and neuron response after nerve injury remain unclear. Here we compared the neuroprotective potentials of SIRT1 activation and SIRT2 inhibition in a mouse model of hypoglossal nerve axotomy...
May 10, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29744899/selenium-and-vitamin-e-supplementation-enhances-the-antioxidant-status-of-spermatozoa-and-improves-semen-quality-in-male-dogs-with-lowered-fertility
#18
A Domosławska, S Zdunczyk, M Franczyk, M Kankofer, T Janowski
Studies showed a beneficial effect of supplementation with selenium (Se) and vitamin E on semen quality. The aim of the study was to investigate the effect of Se and vitamin E supplementation on the antioxidant status of spermatozoa and semen quality in dogs with lowered fertility. Ten dogs were supplemented daily with Se (6 μg/kg organic Se yeast) and vitamin E (5 mg/kg) per os for 60 days. Control group consisted of 10 males without the supplementation. Semen was collected on day 0, 30, 60 and 90. Sperm quality parameters were evaluated using CASA and a microscope...
May 9, 2018: Andrologia
https://www.readbyqxmd.com/read/29744747/the-dynamics-of-pheromone-gland-synthesis-and-release-a-paradigm-shift-for-understanding-sex-pheromone-quantity-in-female-moths
#19
Stephen P Foster, Karin G Anderson, Jérôme Casas
Moths are exemplars of chemical communication, especially with regard to specificity and the minute amounts they use. Yet, little is known about how females manage synthesis and storage of pheromone to maintain release rates attractive to conspecific males and why such small amounts are used. We developed, for the first time, a quantitative model, based on an extensive empirical data set, describing the dynamical relationship among synthesis, storage (titer) and release of pheromone over time in a moth (Heliothis virescens)...
May 10, 2018: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/29743313/ivig-promote-cross-tolerance-against-inflammatory-stimuli-in-vitro-and-in-vivo
#20
Ángeles Domínguez-Soto, Miriam Simón-Fuentes, Mateo de Las Casas-Engel, Víctor D Cuevas, María López-Bravo, Jorge Domínguez-Andrés, Paula Saz-Leal, David Sancho, Carlos Ardavín, Juliana Ochoa-Grullón, Silvia Sánchez-Ramón, Miguel A Vega, Angel L Corbí
IVIg is an approved therapy for immunodeficiency and for several autoimmune and inflammatory diseases. However, the molecular basis for the IVIg anti-inflammatory activity remains to be fully explained and cannot be extrapolated from studies on animal models of disease. We now report that IVIg impairs the generation of human monocyte-derived anti-inflammatory macrophages by inducing JNK activation and activin A production and limits proinflammatory macrophage differentiation by inhibiting GM-CSF-driven STAT5 activation...
May 9, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
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