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https://www.readbyqxmd.com/read/29034773/substrate-dependent-effects-of-molecular-targeted-anticancer-agents-on-activity-of-organic-anion-transporting-polypeptide-1b1
#1
Hiroyoshi Koide, Masayuki Tsujimoto, Ai Takeuchi, Miyu Tanaka, Yoko Ikegami, Mayu Tagami, Syoko Abe, Miki Hashimoto, Tetsuya Minegaki, Kohshi Nishiguchi
1. Organic anion-transporting polypeptide 1B1 (OATP1B1) plays an important role in the hepatic uptake of a broad range of substrate drugs. In vitro experiments show that molecular-targeted agents do not always have similar effects on OATP1B1 activity. 2. The purpose of this study was to clarify whether the effects of molecular-targeted agents on OATP1B1 are substrate-dependent. We used OATP1B1-transfected cells to compare the effects of molecular-targeted agents on OATP1B1-mediated uptake of fluorescein (FL), 2',7'-dichlorofluorescein (DCF), atorvastatin, SN-38, and valsartan...
October 16, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/29032429/dna-damage-and-oxidative-stress-induced-by-seizures-are-decreased-by-anticonvulsant-and-neuroprotective-effects-of-lobeline-a-candidate-to-treat-alcoholism
#2
Liana Dantas da Costa E Silva, Patrícia Pereira, Gabriela Gregory Regner, Fernanda Brião Menezes Boaretto, Cleonice Hoffmann, Pricila Pflüger, Lucas Lima da Silva, Luiza Reinhardt Steffens, Ana Moira Morás, Dinara Jaqueline Moura, Jaqueline Nascimento Picada
The alkaloid lobeline (Lob) has been studied due to its potential use in treatment of drug abuse. This study evaluates the possible anticonvulsant and neuroprotective activities of Lob to obtain new information on its properties that could confirm it as a candidate in the treatment of alcohol addiction. The anticonvulsant effect of Lob was evaluated using a pilocarpine-induced seizure model. In addition, possible neuroprotective effects were investigated measuring DNA damage using the comet assay, assessing free radical levels by dichlorofluorescein diacetate (DCF) oxidation, and measuring the antioxidant potential using the α, α-diphenyl-β-picrylhydrazyl (DPPH) scavenging assay, besides measuring superoxide dismutase (SOD) and catalase (CAT) enzyme activities in brain tissues...
October 14, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29026707/nadph-oxidase-mediates-%C3%AE-amyloid-peptide-induced-neuronal-death-in-mouse-cortical-cultures
#3
Kee-Oh Chay, Kyoung Young Nam Koong, Shinae Hwang, Jong-Keun Kim, Choon Sang Bae
β-Amyloid peptide (Aβ) is the main component of senile plaques in patients with Alzheimer's disease, and is known to be a main pathogenic factor of the disease. Recent evidence indicates that activation of NADPH oxidase (NOX) in microglia or astrocytes may be a source of Aβ-induced reactive oxygen species (ROS). We investigated the role of neuronal NOX in Aβ-induced neuronal death in mouse mixed cortical cultures. Cell death was assessed by measuring lactate dehydrogenase efflux to bathing media 24 or 48 hr after exposure to Aβ25-35, a fragment of Aβ with an equivalent neurotoxic effect...
September 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/28962525/from-the-cover-manganese-and-rotenone-induced-oxidative-stress-signatures-differ-in-ipsc-derived-human-dopamine-neurons
#4
M Diana Neely, Carrie Ann Davison, Michael Aschner, Aaron B Bowman
Parkinson's disease (PD) is the result of complex interactions between genetic and environmental factors. Two chemically distinct environmental stressors relevant to PD are the metal manganese and the pesticide rotenone. Both are thought to exert neurotoxicity at least in part via oxidative stress resulting from impaired mitochondrial activity. Identifying shared mechanism of action may reveal clues towards an understanding of the mechanisms underlying PD pathogenesis. Here we compare the effects of manganese and rotenone in human-induced pluripotent stem cells-derived postmitotic mesencephalic dopamine neurons by assessing several different oxidative stress endpoints...
October 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28948515/differential-molecular-targets-for-neuroprotective-effect-of-chlorogenic-acid-and-its-related-compounds-against-glutamate-induced-excitotoxicity-and-oxidative-stress-in-rat-cortical-neurons
#5
Olfa Rebai, Manel Belkhir, María Victoria Sanchez-Gomez, Carlos Matute, Sami Fattouch, Mohamed Amri
The present study has been designed to explore the molecular mechanism and signaling pathway targets of chlorogenic acid (CGA) and its main hydrolysates, caffeic (CA) and quinic acid in the protective effect against glutamate-excitotoxicity. For this purpose 8-DIV cortical neurons in primary culture were exposed to 50 μM L-glutamic acid plus 10 µM glycine, with or without 10-100 μM tested compounds. Chlorogenic acid and caffeic acid via their antioxidant properties inhibited cell death induced by glutamate in dose depended manner...
September 25, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28932096/an-analytic-model-for-throughput-optimal-distributed-coordination-function-to-dcf
#6
Emma Fitzgerald, Ulf Körner, Bjorn Landfeldt
TO-DCF, a new backoff scheme for 802.11, has the potential to significantly increase throughput in dense wireless LANs while also opportunistically favouring nodes with heavier traffic loads and/or better channel conditions. In this paper we present an analytical model to investigate the behaviour and performance of the TO-DCF protocol with regards to operating parameters such as the number of nodes, the contention window size and the backoff countdown probabilities. We then compare numerical results from an implementation of our model with simulations...
2017: Telecommunication Systems
https://www.readbyqxmd.com/read/28931831/hyperhomocysteinemia-alters-retinal-endothelial-cells-barrier-function-and-angiogenic-potential-via-activation-of-oxidative-stress
#7
Riyaz Mohamed, Isha Sharma, Ahmed S Ibrahim, Heba Saleh, Nehal M Elsherbiny, Sadanand Fulzele, Khaled Elmasry, Sylvia B Smith, Mohamed Al-Shabrawey, Amany Tawfik
Hyperhomocysteinemia (HHcy) is associated with several human visual disorders, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD). Breakdown of the blood-retinal barrier (BRB) is linked to vision loss in DR and AMD. Our previous work revealed that HHcy altered BRB in retinal endothelial cells in vivo. Here we hypothesize that homocysteine (Hcy) alters retinal endothelial cell barrier function and angiogenic potential via activation of oxidative stress. Human retinal endothelial cells (HRECs) treated with and without different concentrations of Hcy showed a reduction of tight junction protein expression, increased FITC dextran leakage, decreased transcellular electrical resistance and increased angiogenic potential...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28930695/metabolomics-assessment-of-the-effects-of-diclofenac-exposure-on-mytilus-galloprovincialis-potential-effects-on-osmoregulation-and-reproduction
#8
Bénilde Bonnefille, Elena Gomez, Mellis Alali, David Rosain, Hélène Fenet, Frédérique Courant
The presence of pharmaceutically active compounds in aquatic environments has become a major concern over the past 20years. Elucidation of their mode of action and effects in non-target organisms is thus now a major ecotoxicological challenge. Diclofenac (DCF) is among the pharmaceutical compounds of interest based on its inclusion in the European Union Water Framework Directive Watch List. In this study, our goal was to investigate the potential of a metabolomic approach to acquire information without any a priori hypothesis about diclofenac effects on marine mussels...
September 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28928512/determination-of-antioxidant-activity-of-hibiscus-sabdariffa-and-croton-caudatus-in-saccharomyces-cerevisiae-model-system
#9
Pattnaik Subhaswaraj, M Sowmya, V Bhavana, Madhu Dyavaiah, Busi Siddhardha
From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of Hibiscus sabdariffa and Croton caudatus were evaluated for free radical scavenging activity in Saccharomyces cerevisiae model system. H. sabdariffa and C. caudatus showed tremendous DPPH free radical scavenging potential with an IC50 value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28917867/nanocrystalline-cellulose-isolated-from-discarded-cigarette-filters
#10
Segun A Ogundare, Vashen Moodley, Werner E van Zyl
We report the isolation of nanocrystalline cellulose (NCC) produced from discarded cigarette filters (DCF). The DCF were processed into cellulose via ethanolic extraction, hypochlorite bleaching, alkaline deacetylation, and then converted into NCC by sulfuric acid hydrolysis. The morphological structures of the isolated NCC established with TEM showed that the nanocrystals were needle-like with a mean length of 143nm. FEGSEM showed the morphological transition of the micro-sized DCF to self-assembled NCC while EDX revealed the presence of Ti (as TiO2) in DCF, which was retained in the NCC...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28915605/hsp90-inhibitor-17-dmag-exerts-anticancer-effects-against-gastric-cancer-cells-principally-by-altering-oxidant-antioxidant-balance
#11
Jeong Goo Kim, Sang Chul Lee, Ok-Hee Kim, Kee-Hwan Kim, Kyo Young Song, Sang Kuon Lee, Byung Jo Choi, Wonjun Jeong, Say-June Kim
Heat shock protein 90 (HSP90) stabilizes numerous oncoproteins and, therefore, its inhibition has emerged as a promising antineoplastic strategy for diverse malignancies. In this study, we determined the therapeutic effects and mechanisms of action of a specific HSP90 inhibitor, 17-dimethylamino-ethylamino-17-demethoxygeldanamycin (17-DMAG), in gastric cancer cell lines (AGS, SNU-1, and KATO-III), patient-derived tissues, and a mouse xenograft model. 17-DMAG exerted anticancer effects against gastric cancer cells, manifested by significantly decreased proliferation rates (P < 0...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28894054/-surgical-treatment-for-synchronous-double-cancer-of-the-thoracic-esophagus-and-lung
#12
Tetsuya Tajima, Shigeo Haruki, Noriaki Takiguchi, Shinsuke Usui, Koji Ito, Akiyo Matsumoto, Takuya Onuki, Masaharu Inagaki, Keiko Suzuki
We herein report 2 cases of radical operation for synchronous double cancer of the thoracic esophagus and each side of the lung. Case 1:A 71-year-old woman with synchronous double cancer of the thoracic esophagus (Mt, T3N2M0, Stage III) and right lung (M, T2aN0M0, Stage I B) underwent esophagectomy concomitantly with right middle lobectomy through right thoracotomy (single-stage operation) after 2 courses of systemic chemotherapy with docetaxel, cisplatin and 5-fluorouracil( DCF regimen). Case 2:A 72-year-old man with synchronous double cancer of the thoracic esophagus( MtLt, T3N2M0, Stage III) and left lung( U, T1aN0M0, Stage I A) underwent 2-stage operation after 2 courses of the DCF therapy...
September 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28889381/oxidative-stress-in-urothelial-carcinogenesis-measurements-of-protein-carbonylation-and-intracellular-production-of-reactive-oxygen-species
#13
Patcharawalai Whongsiri, Suchittra Phoyen, Chanchai Boonla
Oxidative stress contributes substantially to urothelial carcinogenesis. Its extent can be assessed by measurements of reactive species (mainly reactive oxygen species (ROS)), oxidatively modified damage products, and levels of various antioxidants. We presented herein the methods for the measurement of protein carbonyl content and intracellular production of ROS. Protein carbonyl is the most commonly used indicator of protein oxidation because it is early formed and relatively stable under oxidative stress...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28862068/novel-transparent-collagen-film-patch-derived-from-duck-s-feet-for-tympanic-membrane-perforation
#14
Soo Hyeon Kim, Ho Jun Lee, Ji-Chul Yoo, Hyun Jung Park, Ju Yeon Jeong, Ye Bin Seo, Md Tipu Sultan, Soon Hee Kim, Ok Joo Lee, Chan Hum Park
To increase healing rate of tympanic membrane (TM) perforations, patching procedure has been commonly conducted. Biocompatible, biodegradable patching materials which is not limited across cultures is needed. The authors evaluated the effectiveness of novel transparent duck's feet collagen film (DCF) patch in acute traumatic TM perforation. This procedure was compared with spontaneous healing and paper patching. Cell proliferation features were observed in paper and DCF patches. 48 TMs of 24 rats were used for animal experiment, perforations were made on each TMs, and divided into three groups according to treatment modality...
September 1, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28841909/docetaxel-cisplatin-and-5-fluorouracil-dcf-chemotherapy-in-the-treatment-of-metastatic-or-unresectable-locally-recurrent-anal-squamous-cell-carcinoma-a-phase-ii-study-of-french-interdisciplinary-gercor-and-ffcd-groups-epitopes-hpv02-study
#15
Stefano Kim, Marine Jary, Thierry André, Véronique Vendrely, Bruno Buecher, Eric François, François-Clément Bidard, Sarah Dumont, Emmanuelle Samalin, Didier Peiffert, Simon Pernot, Nabil Baba-Hamed, Farid El Hajbi, Olivier Bouché, Jérôme Desrame, Aurélie Parzy, Mustapha Zoubir, Christophe Louvet, Jean-Baptiste Bachet, Thierry Nguyen, Meher Ben Abdelghani, Denis Smith, Christelle De La Fouchardière, Thomas Aparicio, Jaafar Bennouna, Jean-Marc Gornet, Marion Jacquin, Franck Bonnetain, Christophe Borg
BACKGROUND: The squamous cell carcinoma of the anus (SCCA) is a rare disease, but its incidence is markedly increasing. About 15% of patients are diagnosed at metastatic stage, and more than 20% with a localized disease treated by chemoradiotherapy (CRT) will recur. In advanced SCCA, cisplatin and 5-fluorouracil (CF) combination is the standard option but complete response is a rare event and the prognosis remains poor with most disease progression occurring within the first 12 months...
August 25, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28835449/fluorescence-quenching-by-metal-centered-porphyrins-and-poryphyrin-enzymes
#16
Kenneth R Olson, Yan Gao, Faihaan Arif, Kanika Arora, Shivali Patel, Eric DeLeon, Karl D Straub
Fluorescence spectroscopy and microscopy have been used extensively to monitor biomolecules, especially reactive oxygen species (ROS) and, more recently, reactive sulfide (RSS) species. Nearly all fluorophores are either excited by or emit light between 450 and 550 nm, which is similar to the absorbance of heme proteins and metal-centered porphyrins. Here we examined the effects of catalase (Cat), reduced and oxidized hemoglobin (Hb and metHb), albumin (alb), manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP), iron protoporphyrin IX (hemin), and copper protoporphyrin IX (CuPPIX) on the fluorescence properties of fluorescein...
October 1, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28810950/clofibrate-attenuates-ros-production-by-lipid-overload-in-cultured-rat-hepatoma-cells
#17
Yufei Chen, Wei Li, Guqi Wang, Frank J Burczynski
PURPOSE: To investigate the effect of clofibrate on inducing liver fatty acid binding protein (FABP1) following a high-fat load in a hepatocyte cell culture model. METHODS: Rat hepatoma cells (CRL-1548) were treated with a fatty acid (FA) mixture consisting of oleate:palmitate (2:1) in the presence of 3% albumin. Cells were treated with 0, 0.5, 1, 2, or 3 mM FA for 24 and 48 hr, or further treated with 500 µM clofibrate (CLO) to induce FABP1 levels. Cytotoxicity was determined using the WST-1 assay...
2017: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28764332/pharmaceutical-removal-from-water-effluents-by-adsorption-on-activated-carbons-a-monte-carlo-simulation-study
#18
Daniel Bahamon, Lourdes F Vega
Adsorption on activated carbons of five pharmaceutical molecules (ibuprofen, diclofenac, naproxen, paracetamol, and amoxicillin) in aqueous mixtures has been investigated by molecular simulations using the Grand Canonical Monte Carlo (GCMC) method. A virtual nanoporous carbon model based on polyaromatic units with defects and polar-oxygenated sites was used for this purpose. The simulation results show excellent agreement with available experimental data. The adsorption capacities of the carbons for the five drugs were quite different and were linked, essentially, to their molecular dimensions and atom affinities...
August 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28758971/tricetin-induces-apoptosis-of-human-leukemic-hl-60-cells-through-a-reactive-oxygen-species-mediated-c-jun-n-terminal-kinase-activation-pathway
#19
Ming-Hsien Chien, Jyh-Ming Chow, Wei-Jiunn Lee, Hui-Yu Chen, Peng Tan, Yu-Ching Wen, Yung-Wei Lin, Pei-Ching Hsiao, Shun-Fa Yang
Tricetin is a dietary flavonoid with cytostatic properties and antimetastatic activities in various solid tumors. The anticancer effect of tricetin in nonsolid tumors remains unclear. Herein, the molecular mechanisms by which tricetin exerts its anticancer effects on acute myeloid leukemia (AML) cells were investigated. Results showed that tricetin inhibited cell viability in various types of AML cell lines. Tricetin induced morphological features of apoptosis such as chromatin condensation and phosphatidylserine (PS) externalization, and significantly activated proapoptotic signaling including caspase-8, -9, and -3 activation and poly(ADP-ribose) polymerase (PARP) cleavage in HL-60 AML cells...
July 31, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28750732/ni-functionalized-submicron-mesoporous-silica-particles-as-a-sorbent-for-metal-affinity-chromatography
#20
Dmitry A Kurdyukov, Ekaterina N Chernova, Yana V Russkikh, Daniil A Eurov, Vasily V Sokolov, Anna A Bykova, Vladimir V Shilovskikh, Olga A Keltsieva, Eugenii V Ubyivovk, Yuri A Anufrikov, Anna V Fedorova, Artem A Selyutin, Nicolay G Sukhodolov, Ekaterina P Podolskaya, Valery G Golubev
In this research, a novel IMAC sorbent with high specificity for chlorine-containing compounds was developed. Ni-functionalized monodisperse spherical mesoporous silica particles of 500±25nm diameter were synthesized and their metal affinity properties were studied with the use of diclofenac as the model substance. The particles were aggregatively stable in the pH range of 3-12. The sorbent demonstrated a high adsorption capacity (0.60±0.06μg of DCF per 1mg of the sorbent) and high adsorption/desorption rate (20 and 5min was enough for the sorbent saturation and desorption of DCF, correspondingly)...
July 15, 2017: Journal of Chromatography. A
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