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https://www.readbyqxmd.com/read/28528506/exposure-of-marine-mussels-to-diclofenac-modulation-of-prostaglandin-biosynthesis
#1
Frédérique Courant, Lauren Arpin-Pont, Bénilde Bonnefille, Sébastien Vacher, Marina Picot-Groz, Elena Gomez, Hélène Fenet
Human pharmaceuticals, such as nonsteroidal anti-inflammatory drugs (NSAIDs), are an emerging threat to marine organisms. NSAIDs act through inhibition of cyclooxygenase (COX) conversion of arachidonic acid into prostaglandins. One experiment was carried out whereby marine mussels were exposed for 72 h to 1 and 100 μg/L diclofenac (DCF). A specific and sensitive method using liquid chromatography high-resolution tandem mass spectrometry was developed to quantify DCF in mussel tissues. The developed method could also clearly identify and quantify COX products, i...
May 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28522175/pharmacological-inhibition-of-soluble-epoxide-hydrolase-or-genetic-deletion-reduces-diclofenac-induced-gastric-ulcers
#2
Sumanta Kumar Goswami, Amelia Ann Rand, Debin Wan, Jun Yang, Bora Inceoglu, Melany Thomas, Christophe Morisseau, Guang-Yu Yang, Bruce D Hammock
AIMS: This research was conducted to evaluate the hypothesis that gastric ulcers caused by the NSAID diclofenac sodium (DCF) can be prevented by the soluble epoxide hydrolase inhibitor TPPU. MAIN METHODS: Mice were administered a single dose of 10, 30 or 100mg/kg of DCF. Once an ulcerative dose of DCF was chosen, mice were pretreated with TPPU for 7days at 0.1mg/kg to evaluate anti-ulcer effects of the sEH inhibitor on anatomy, histopathology, pH, inflammatory markers and epithelial apoptosis of stomachs...
May 15, 2017: Life Sciences
https://www.readbyqxmd.com/read/28511040/removal-of-selected-ppcps-edcs-and-antibiotic-resistance-genes-in-landfill-leachate-by-a-full-scale-constructed-wetlands-system
#3
Xinzhu Yi, Ngoc Han Tran, Tingru Yin, Yiliang He, Karina Yew-Hoong Gin
Landfill leachate could be a significant source of emerging contaminants (ECs) and antibiotic resistance genes (ARGs) into the environment. This study provides the first information on the occurrence of selected ECs and ARGs in raw leachate from 16-year old closed landfill site in Singapore. Among the investigated ECs, acetaminophen (ACT), bisphenol A (BPA), clofibric acid (CA), caffeine (CF), crotamiton (CTMT), diclofenac (DCF), N,N-diethyl-m-toluamide (DEET), gemfibrozil (GFZ), lincomycin (LIN), salicylic acid (SA), and sulfamethazine (SMZ) were the most frequently detected compounds in raw landfill leachate...
May 8, 2017: Water Research
https://www.readbyqxmd.com/read/28504156/poly-n-methacryloyl-glycine-nanocellulose-composites-as-ph-sensitive-systems-for-controlled-release-of-diclofenac
#4
Louise Saïdi, Carla Vilela, Helena Oliveira, Armando J D Silvestre, Carmen S R Freire
The present study reports the development of non-cytotoxic and pH-sensitive nanostructured membranes consisting of a polymer with amino acid pending moieties and bacterial nanocellulose (BC). The nanocomposites were prepared through a simple methodology under green reaction conditions. The obtained materials display good thermal stability (up to 200°C), viscoelastic (storage modulus>700MPa) and mechanical (Young's modulus=3.5-4.9GPa) properties, together with high water uptake capacity. The results of the in vitro MTT assay showed that the nanocomposites are non-cytotoxic to HaCaT cells for 72h...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28504001/n-acetylcysteine-potentiates-diclofenac-toxicity-in-saccharomyces-cerevisiae-stronger-potentiation-in-abc-transporter-mutant-strains
#5
Houssein Al-Attrache, Hala Chamieh, Monzer Hamzé, Isabelle Morel, Samir Taha, Ziad Abdel-Razzak
Diclofenac (DCF) adverse reactions involve diverse mechanisms in different models. We recently demonstrated that DCF-induced toxicity in HepaRG decreases as they express DCF-metabolizing enzymes. DCF metabolism promotes toxicity in Saccharomyces cerevisiae expressing heterologous cytochromes-P450. N-Acetylcysteine (NAC) is used to treat diverse medical conditions due to its multiple properties (antioxidant, metal chelator, thiol-disulfide disruption). The latter property accounts for its mucolytic effects and broadens its potential molecular targets to signal transduction proteins, ABC transporters and others...
May 15, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28500745/advanced-oxidation-protein-products-induce-hepatocyte-epithelial-mesenchymal-transition-via-a-ros-dependent-tgf-%C3%AE-smad-signaling-pathway
#6
Shibo Sun, Fang Xie, Qifan Zhang, Zhonglin Cui, Xinsheng Cheng, Feng Zhong, Kun He, Jie Zhou
Epithelial-mesenchymal transition (EMT) occurs during the progression of liver fibrosis in response to chronic liver injury. However, the molecular mechanism underlying the regulation of hepatocyte EMT remains unclear. The aim of this study was to determine whether advanced oxidation protein products (AOPP) had an effect on hepatocyte EMT. The human L02 hepatocyte cell line and hepatocytes from normal Sprague-Dawley rats were challenged with AOPP treatment in both in vitro and in vivo studies. The expression of cell and molecular markers of EMT in L02 hepatocytes were studied using Western blotting, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays...
May 13, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28498129/photodegradation-of-diclofenac-in-aqueous-solution-by-simulated-sunlight-irradiation-kinetics-thermodynamics-and-pathways
#7
N Zhang, J M Li, G G Liu, X L Chen, K Jiang
Diclofenac (DCF) is one of the most frequently detected pharmaceuticals in various water samples. This paper studied the effects of aquatic environmental factors (pH, temperature and dissolved organic matter) on photodegradation of DCF under simulated sunlight. The results demonstrate that degradation pathways proceed via pseudo first-order kinetics in all cases and the photodegradation of DCF by simulated sunlight. Thermodynamic study indicated that the photodegradation course is spontaneous, exothermic and irreversible...
May 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28494450/stachydrine-protects-against-pressure-overload-induced-cardiac-hypertrophy-by-suppressing-autophagy
#8
Tong-Tong Cao, Hui-Hua Chen, Zhiwei Dong, Yan-Wu Xu, Pei Zhao, Wei Guo, Hong-Chang Wei, Chen Zhang, Rong Lu
BACKGROUND: Autophagy is required for the maintenance of cardiomyocyte homeostasis. However, excessive autophagy plays a maladaptive role in pressure overload-induced heart failure. To identify mechanisms by which Stachydrine inhibits pressure overload-induced cardiac hypertrophy, we determined inhibitory activities against activation of NADPH oxidase, reactive oxygen species(ROS) production and excessive activation of autophagy. METHODS: Stachydrine was administered intragastrically to Wistar rats after Transverse aortic constriction(TAC) and H9c2 cells were treated with Stachydrine after Angiotension II stimulation...
May 11, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28494300/behaviour-of-the-main-nonsteroidal-anti-inflammatory-drugs-in-a-membrane-bioreactor-treating-urban-wastewater-at-high-hydraulic-and-sludge-retention-time
#9
D M González-Pérez, J I Pérez, M A Gómez
The behaviour and removal efficiency of ibuprofen (IBU), diclofenac (DCF), ketoprofen (KPF), and naproxen (NPX) during the real urban wastewater treatment by an experimental full-scale MBR working at high sludge and hydraulic retention time (SRT, HRT) were determined. The MBR worked in denitrification/nitrification conformation at 35.4h of HRT (Q=0.45m(3)/h), 37 d of SRT and a recirculation flow rate of 4Q. The experiments were made under steady-state conditions, reaching a biodegradable organic matter removal higher than 99...
April 26, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28479722/influence-of-capsaicin-on-inflammatory-cytokines-induced-by-lipopolysaccharide-in-myoblast-cells-under-in-vitro-environment
#10
Ke Shang, Touseef Amna, Musarat Amina, Nawal M Al-Musayeib, Salem S Al-Deyab, Inho Hwang
BACKGROUND: ellular damage initiated by reactive oxygen species (ROS) is the main cause of numerous severe diseases and therefore for this reason, the natural antioxidants have note worthy significance in human health. Capsaicin possesses noteworthy analgesic and anti-inflammatory properties. It also possesses healing effects for treatment of arthritis, diabetic neuropathy, gastric lesions, and cardiac excitability that is why it is incorporated in creams and gels. OBJECTIVE: The present study was carried out to estimate the in vitro antioxidant and ROS scavenging activities of capsaicin against muscle precursor cells...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28477257/control-of-emerging-contaminants-by-the-combination-of-electrochemical-processes-and-membrane-bioreactors
#11
Benny Marie B Ensano, Laura Borea, Vincenzo Naddeo, Mark Daniel G de Luna, Vincenzo Belgiorno
This study investigates the removal of selected pharmaceuticals, as recalcitrant organic compounds, from synthetic wastewater using an electro-membrane bioreactor (eMBR). Diclofenac (DCF), carbamazepine (CBZ), and amoxicillin (AMX) were selected as representative drugs from three different therapeutic groups such as anti-inflammatory, anti-epileptic, and antibiotic, respectively. An environmentally relevant concentration (10 μg/L) of each compound was spiked into the synthetic wastewater, and then, the impact of appending electric field on the control of membrane fouling and the removal of conventional contaminants and pharmaceutical micropollutants were assessed...
May 5, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28475971/novel-mononuclear-cu-ii-terpyridine-complexes-impact-of-fused-ring%C3%A2-thiophene-and-thiazole-head-groups-towards-dna-bsa-interaction-%C3%A2-cleavage-and-antiproliferative-activity-on-hepg2-and%C3%A2-triple%C3%A2-negative-cal-51%C3%A2-cell-line
#12
V M Manikandamathavan, M Thangaraj, T Weyhermuller, R P Parameswari, V Punitha, N Narasimha Murthy, Balachandran Unni Nair
Two mononuclear copper (II) terpyridine complexes namely, [Cu(Btptpy) (ClO4)](ClO4) 1, and [Cu(Bttpy) (ClO4)](ClO4) 2, (Btptpy (L1) = 4'-(Benzothiophene)-2,2':6',2″-terpyridine, Bttpy (L2) = 4'-(Benzylthiazolyl)-2,2':6',2″-terpyridine) have been synthesized and characterized. Single crystal X-ray diffraction shows that, both ligands belong to monoclinic crystal system with space group P21/c (L1) and P21/n (L2). Absorption spectral titration, DNA melting study, circular dichroism and viscosity measurement reveal that, complex 1 and 2 bind with DNA through intercalation...
April 15, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28474422/determination-of-antioxidant-potential-of-acacia-nilotica-leaf-extract-in-oxidative-stress-response-system-of-saccharomyces-cerevisiae
#13
Subhaswaraj Pattnaik, Sowmya Mani, Jobina Rajkumari, Sudharshan Sj, Madhu Dyavaiah, Siddhardha Busi
BACKGROUND: From ancient times, plants and plant derived products are used as folkloric medicines in a variety of health disorders owing to their tremendous therapeutic potential. The present study aims to determine the antioxidant efficacy of crude Acacia nilotica extract in oxidative stress response system of Saccharomyces cerevisiae as model organism. RESULTS: A. nilotica showed significant antioxidant activity with an IC50 value of 75.157 and 159.57 µg mL(-1) for DPPH radical and hydroxyl radical scavenging activity respectively at a concentration of 500 µg mL(-1) ...
May 5, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28473598/test-retest-variability-of-lesion-suv-and-lesion-sur-in-18-f-fdg-pet-an-analysis-of-data-from-two-prospective-multicenter-trials
#14
Frank Hofheinz, Ivayla Apostolova, Liane Oehme, Jörg Kotzerke, Jörg van den Hoff
Quantitative assessment of radiation- and chemotherapy response with (18)F-FDG (fluorodeoxyglucose) whole body PET has attracted increasing interest in recent years. In most published work the standardized uptake value (SUV) is utilized for this purpose. In the context of therapy response assessment the reliability of lesion SUVs, notably their test-retest stability, thus becomes of crucial importance. However, in a recent study substantial test-retest variability (TRV) of SUVs was demonstrated. The purpose of the present study was to investigate if the tumor-to-blood standard uptake ratio (SUR) can improve test-retest variability of tracer uptake...
May 4, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28472710/promoted-oxidation-of-diclofenac-with-ferrate-fe-vi-role-of-abts-as-the-electron-shuttle
#15
Huiyu Dong, Zhimin Qiang, Junfeng Lian, Jiuhui Qu
Reaction of Fe(VI) with 2,2'-azino-bis-(3-ethylbenzothiazoline -6-sulfonate) (ABTS) is widely adopted to determine aqueous ferrate (Fe(VI)) concentration based on ABTS(+) formation. Interestingly, this study found that the addition of ABTS could accelerate the oxidation of diclofenac (DCF) by Fe(VI) significantly. Observed first-order rate constant of DCF in the presence of 30μM ABTS was found to be 36.2 folds of that without ABTS, with values of 3.08 and 0.085min(-1), respectively. It was partly attributed to the formation of ABTS(+)...
April 25, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28468627/the-omega-3-polyunsaturated-fatty-acid-docosahexaenoic-acid-inhibits-proliferation-and-progression-of-non-small-cell-lung-cancer-cells-through-the-reactive-oxygen-species-mediated-inactivation-of-the-pi3k-akt-pathway
#16
Yuanqin Yin, Chengguang Sui, Fandong Meng, Ping Ma, Youhong Jiang
BACKGROUND: Docosahexaenoic acid(DHA) inhibits tumor growth and progression in various cancers, including lung cancer. However, the mechanisms involved remain unclear. The aim of this study was to identify the mechanism of DHA in inhibiting progression of non-small cell lung cancer (NSCLC) in vitro. METHODS: The proliferation of A549 was tested by MTT, and cell apoptosis was analysed using flow cytometer. The migration and invasion were examined respectively by wound healing assay and Transwell invasion assay...
May 3, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28467633/a-method-for-rapid-and-sensitive-negative-staining-of-proteins-in-sds-page-using-2-7-dichlorofluorescein
#17
Yuan Zhang, Dongdong Yu, Jiangbo Dai, Xu Wang, Zhongxin Zhu, Yewei Huang, Jia Sun, Zhiwei Chen, Litai Jin, Yang Wang
We report here a rapid and sensitive technique for negative visualization of protein in 1-D and 2-D SDS-PAGE by using 2', 7'-Dichlorofluorescein (DCF), which appeared as transparent and colorless bands in an opaque gel matrix background. For DCF stain, down to 0.1-0.2 ng protein could be easily visualized within 7 min by only 2 steps, and the staining is 4-fold more sensitive than that of Eosin Y (EY) negative stain and glutaraldehyde (GA) silver stain, and 8-fold more sensitive than that of the commonly used Imidazole-zinc (IZ) negative stain...
May 3, 2017: Proteomics
https://www.readbyqxmd.com/read/28461775/dust-particles-induced-intracellular-ca-2-signaling-and-reactive-oxygen-species-in-lung-fibroblast-cell-line-mrc5
#18
Dong Un Lee, Min Jeong Ji, Jung Yun Kang, Sun Young Kyung, Jeong Hee Hong
Epidemiologic interest in particulate matter (PM) is growing particularly because of its impact of respiratory health. It has been elucidated that PM evoked inflammatory signal in pulmonary epithelia. However, it has not been established Ca(2+) signaling mechanisms involved in acute PM-derived signaling in pulmonary fibroblasts. In the present study, we explored dust particles PM modulated intracellular Ca(2+) signaling and sought to provide a therapeutic strategy by antagonizing PM-induced intracellular Ca(2+) signaling in human lung fibroblasts MRC5 cells...
May 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28456771/anti-inflammatory-effects-of-simvastatin-in-nonsteroidal-anti-inflammatory-drugs-induced-small-bowel-injury
#19
E K Kim, J H Cho, A R Jeong, E J Kim, D K Park, K A Kwon, J W Chung, K O Kim, J H Kim, J H Kim, Y J Kim
Small bowel injury can occur as the result of a multifaceted process that includes increased acid secretion, generation of reactive oxygen species, and cyclooxygenase inhibition. However, no effective medication for small bowel ulceration is available. Simvastatin is an important lipid-lowering agent with anti-inflammatory activity. We aimed to validate the effects of simvastatin in vitro and in vivo. In presence or absence of simvastatin, IEC-6 small bowel cell line with 50 ng/ml of tumor nectosis factor α (TNF-α) was investigated by western blotting, qRT-PCR, and DCF-DA assay...
February 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28456064/effects-of-diclofenac-on-the-expression-of-nrf2-and-its-downstream-target-genes-in-mosquito-fish-gambusia-affinis
#20
Shuang Bao, Xiangping Nie, Ruikang Ou, Chao Wang, Peijia Ku, Kaibing Li
Diclofenac (DCF) is one of widely used non-steroidal anti-inflammatory drugs. Recently, this drug has been universally detected in aquatic environment. However, its potential adverse effects and oxidative stress toxic mechanisms on fish remain unclear. In the present study, we first cloned the crucial partial sequences of some key oxidative stress related genes, which include NF-E2-related factor 2 (Nrf2), NAD(P)H: quinoneoxidoreductase (NQO1), glutamate-cysteine ligase catalytic subunit (GCLC), Cu-Zn superoxide dismutase (SOD2), catalase (CAT), alpha-glutathione S-transferase (GSTA), and UDP-glucuronosyltransferases (UGT) in mosquito fish (Gambusia affinis)...
April 20, 2017: Aquatic Toxicology
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