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https://www.readbyqxmd.com/read/28525324/in-vitro-effect-of-leptin-on-anterior-pituitary-cells-lh-secretory-activity-during-early-pregnancy-in-pig
#1
G Siawrys, A Gajewska
Leptin modulates reproductive activity but its potential influence on LH secretion from anterior pituitary (AP) cells during implantation period in pigs (days 14-16 of pregnancy) remained unexplored. This study focused on determination whether leptin affects basal and GnRH-induced LH secretion and intracellular accumulation and whether leptin receptor (OB-Rb) mRNA is expressed in the AP gland during implantation in pigs. Four individual AP glands were developed into separate primary cultures. 2×105 cells/ml were preincubated (72 h) and next, for 3...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28524342/the-effect-of-vitamin-d-supplementation-on-hormonal-and-glycaemic-profile-of-patients-with-pcos-a-meta-analysis-of-randomised-trials
#2
Vasilios Pergialiotis, Nikoleta Karampetsou, Periklis Panagopoulos, Eftihios Trakakis, Nikolaos Papantoniou
BACKGROUND: Vitamin D deficiency is frequently manifested in women with polycystic ovarian syndrome (PCOS). To date, supplementation of deficient patients has not been correlated with the hormonal and metabolic status of these patients. PURPOSE: We aimed to investigate the impact of vitamin D supplementation on the hormonal and metabolic profile of PCOS women. MATERIALS AND METHODS: We searched Medline, Scopus, ClinicalTrials.gov and Cochrane Central Register databases for published randomised controlled trials...
May 19, 2017: International Journal of Clinical Practice
https://www.readbyqxmd.com/read/28522756/influence-of-sex-menstrual-cycle-and-oral-contraceptives-on-cerebrovascular-resistance-and-cardiorespiratory-function-during-valsalva-or-standing
#3
Syed Abidi, Misha Nili, Stephania Serna, Simon Kim, Christopher Hazlett, Heather Edgell
Women experience orthostatic intolerance more than men, and experience faintness more in the early follicular (i.e. low hormone (LH)) compared to the luteal (i.e. high hormone (HH)) phase of the menstrual cycle. Men (n=13, age: 25.8±1.8) and women in the LH (day 2-5; placebo) and HH) (day 18-24; high dose) phases of the menstrual cycle with (OC; n=14, age: 22.0±0.8) or without oral contraceptives (NOC; n=12, age: 21.8±0.5) underwent the Valsalva maneuver and a supine-sit-stand protocol. Blood pressure, normalized stroke volume (SVi), cardiac output index (Qi), heart rate (HR), end-tidal carbon dioxide (ET-CO2), and middle cerebral artery blood velocity (MCA) were measured...
May 18, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28522409/neonatal-exposure-to-17%C3%AE-ethynyl-estradiol-ee-disrupts-follicle-development-and-reproductive-hormone-profiles-in-female-rats
#4
Haolin Zhang, Kazuyoshi Taya, Kentaro Nagaoka, Midori Yoshida, Gen Watanabe
Toxic effects induced by exposure to endocrine-disrupting chemicals during fetal and neonatal periods can be irreversible and exert effects throughout an animal's entire life. Our previous study showed that neonatal exposure to 17α-ethynyl estradiol (EE) induced irregular estrous cycle in adults. To uncover the reason for the delayed effect after neonatal exposure to EE, reproductive parameters including ovarian weight, ovarian steroidogenesis, and hormonal profiles were investigated in developing female rats...
May 15, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28522354/corrigendum-to-differential-effects-of-the-persistent-ddt-metabolite-methylsulfonyl-dde-in-nonstimulated-and-lh-stimulated-neonatal-porcine-leydig-cells-toxicology-and-applied-pharmacology-267-2013-247-255
#5
Cesilie Granum Castellanos, Irene Beate Sørvik, Marte Bruu Tanum, Steven Verhaegen, Ingvar Brandt, Erik Ropstad
No abstract text is available yet for this article.
May 15, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28522293/direct-action-of-adiponectin-ameliorates-increased-androgen-synthesis-and-reduces-insulin-receptor-expression-in-the-polycystic-ovary
#6
Anusha Singh, Puran Bora, Amitabh Krishna
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder of reproductive age females, leading to infertility. Despite extensive research, the exact pathophysiology and treatment are lacking. The aim of the current study was to evaluate the direct role of adiponectin either alone or together with luteinizing hormone (LH) on sex hormone synthesis and changes in insulin receptor (IR) in the ovary of dehydroepiandrosterone (DHEA)-treated PCOS-mice. Immunohistochemical study showed decreased expression of adiponectin receptor (AdipoR1) in PCOS-ovary which correlated with increased synthesis of androgen and decreased expression of IR...
May 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28521851/regulation-of-interleukin-11-expression-in-ovulatory-follicles-of-the-rat-ovary
#7
You-Jee Jang, You-Jee Jang, Jae-Il Park, Jae-Il Park, Seong-Eun Jeong, Seong-Eun Jeong, You-Mi Seo, You-Mi Seo, Phuong T M Dam, Phuong T M Dam, Young-Woo Seo, Young-Woo Seo, Bum-Chae Choi, Bum-Chae Choi, Sang-Jin Song, Sang-Jin Song, Sang-Young Chun, Sang-Young Chun, Moon-Kyoung Cho, Moon-Kyoung Cho
The aim of the present study was to examine the regulation of interleukin (IL)-11 expression, as well as the role of IL-11, during ovulation in gonadotropin-primed immature rats. Injection of equine chorionic gonadotropin (eCG), followed by human CG (hCG) to induce superovulation stimulated expression of the Il11 gene in theca cells within 6h, as revealed by northern blot and in situ hybridisation analyses. Real-time reverse transcription-polymerase chain reaction analysis showed that the IL-11 receptor, α subunit gene was expressed in granulosa and theca cells and that injection of hCG had no effect on its expression...
May 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28520651/laparoscopic-management-of-hepatocellular-carcinoma-a-critical-reappraisal
#8
David Fuks, Luca Aldrighetti, Long R Jiao, Go Wakabayashi, Paolo Limongelli
Following the introduction of the first laparoscopic liver resection, after ∼25 years, a critical reappraisal seems to be warranted. Liver resection represents the first choice for curing early hepatocellular carcinoma (HCC) allowing a curative chance also in selected patients with intermediate stage tumors. The criteria for liver resectability by laparoscopy remains the same for open surgery, which is oncological criteria (absence of extrahepatic tumor location; completely resectable R0 resection), anatomic (resectability of involved segment with its own blood surely and biliary drainage; absence of vascular invasion of portal or hepatic veins) and technical (possibility to leave in place a residual volume ≥40%)...
May 17, 2017: Surgical Laparoscopy, Endoscopy & Percutaneous Techniques
https://www.readbyqxmd.com/read/28515683/a-population-of-projection-neurons-that-inhibits-the-lateral-horn-but-excites-the-antennal-lobe-through-chemical-synapses-in-drosophila
#9
Kazumichi Shimizu, Mark Stopfer
In the insect olfactory system, odor information is transferred from the antennal lobe (AL) to higher brain areas by projection neurons (PNs) in multiple AL tracts (ALTs). In several species, one of the ALTs, the mediolateral ALT (mlALT), contains some GABAergic PNs; in the Drosophila brain, the great majority of ventral PNs (vPNs) are GABAergic and project through this tract to the lateral horn (LH). Most excitatory PNs (ePNs), project through the medial ALT (mALT) to the mushroom body (MB) and the LH. Recent studies have shown that GABAergic vPNs play inhibitory roles at their axon terminals in the LH...
2017: Frontiers in Neural Circuits
https://www.readbyqxmd.com/read/28515223/associations-between-male-reproductive-characteristics-and-the-outcome-of-assisted-reproductive-technology-art
#10
Zhangshun Liu, Xiaohong Shi, Lihong Wang, Yan Yang, Qiang Fu, Minfang Tao
This study was designed to investigate the relationships between indicators of male body-mass index (BMI), age, reproductive hormone levels, semen parameters and the outcomes of assisted reproductive technology (ART). The clinical data were collected from 636 couples who underwent ART at the reproductive center involved in our study. Pearson's correlation or Spearman rank correlation was applied to establish the relevant correlation coefficients. The correlation between influence factors' and pregnancy outcomes was analyzed using the Logistic regression model...
May 17, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28511700/a-new-easy-to-make-pectin-honey-hydrogel-enhances-wound-healing-in-rats
#11
Gessica Giusto, Cristina Vercelli, Francesco Comino, Vittorio Caramello, Massimiliano Tursi, Marco Gandini
BACKGROUND: Honey, alone or in combination, has been used for wound healing since ancient times and has reemerged as a topic of interest in the last decade. Pectin has recently been investigated for its use in various biomedical applications such as drug delivery, skin protection, and scaffolding for cells. The aim of the present study was to develop and evaluate a pectin-honey hydrogel (PHH) as a wound healing membrane and to compare this dressing to liquid honey. METHODS: Thirty-six adult male Sprague-Dawley rats were anesthetized and a 2 × 2 cm excisional wound was created on the dorsum...
May 16, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28505106/human-chorionic-gonadotropin-the-pregnancy-hormone-and-more
#12
REVIEW
Charalampos Theofanakis, Petros Drakakis, Alexandros Besharat, Dimitrios Loutradis
To thoroughly review the uses of human chorionic gonadotropin (hCG) related to the process of reproduction and also assess new, non-traditional theories. Review of the international literature and research studies. hCG and its receptor, LH/CGR, are expressed in numerous sites of the reproductive tract, both in gonadal and extra-goanadal tissues, promoting oocyte maturation, fertilization, implantation and early embryo development. Moreover, hCG seems to have a potential role as an anti-rejection agent in solid organ transplantation...
May 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28504984/melding-a-high-risk-patient-for-continuous-flow-left-ventricular-assist-device-into-a-low-risk-patient
#13
Javier Amione-Guerra, Ana S Cruz-Solbes, Hilda Gonzalez Bonilla, Jerry D Estep, Ashrith Guha, Arvind Bhimaraj, Erik E Suarez, Brian A Bruckner, Guillermo Torre-Amione, Myung H Park, Barry H Trachtenberg
The model for end-stage liver disease (MELD) has been used as a predictor of mortality after left ventricular assist device (LVAD) placement. However, improvement or worsening of MELD and how those changes affect outcomes is unknown. We performed a retrospective analysis of 244 patients implanted with a continuous flow (CF) LVAD. Patients were dichotomized at admission into low- or high-risk categories using a cutoff of MELD ≥ 19, and they were reclassified at day of implant forming four groups: Group LL (remained low risk), LH (worsened to high risk), HH (remained high risk), and HL (improved to low risk)...
May 10, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28502859/the-significance-of-estradiol-metabolites-in-human-corpus-luteum-physiology
#14
REVIEW
Luigi Devoto, Soledad Henríquez, Paulina Kohen, Jerome F Strauss
The human corpus luteum (CL) is a temporary endocrine gland derived from the ovulated follicle. Its formation and limited lifespan is critical for steroid hormone production required to support menstrual cyclicity, endometrial receptivity for successful implantation, and the maintenance of early pregnancy. Endocrine and paracrine-autocrine molecular mechanisms associated with progesterone production throughout the luteal phase are critical for the development, maintenance, regression, and rescue by hCG which sustains CL function into early pregnancy...
May 11, 2017: Steroids
https://www.readbyqxmd.com/read/28501602/soluble-epoxide-hydrolase-inhibitory-activity-of-components-from-leonurus-japonicus
#15
Hyun Hee Leem, Ga Young Lee, Ji Sun Lee, Hanna Lee, Jang Hoon Kim, Young Ho Kim
One new compound, 10-methoxy-leonurine (1), and four known compounds (2-5) were purified by silica gel, C-18, and Sephadex LH-20 column chromatography from Leonurus japonicus. Their structures were elucidated using one-dimensional (1D)/two-dimensional (2D)-nuclear magnetic resonance (NMR), high-resolution (HR)-electrospray ionization (ESI) mass spectrometry (MS). The compounds were evaluated to determine their inhibition of the catalysis of soluble epoxide hydrolase (sEH). According to the results from in vitro analyses, compounds 1 and 2, which contain guanidine and flavonoid (3), were determined to be potential inhibitors of this enzyme...
May 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28501545/betamethasone-causes-intergenerational-reproductive-impairment-in-male-rats
#16
Cibele Dos Santos Borges, Taina Louise Pacheco, Katiussia Pinho da Silva, Fábio Henrique Fernandes, Mary Gregory, André Sampaio Pupo, Daisy Maria F Salvadori, Daniel G Cyr, Wilma De G Kempinas
Prenatal betamethasone (BM) exposure in rats negatively impacts sperm quality and male fertility. Studies have shown that BM can cause multi-generational effects on the pituitary-adrenal-axis of rats. The objective of this study was to assess the reproductive development and fertility of male rats (F2) whose fathers (F1) were exposed to BM (0.1mg/kg) on gestational days 12, 13, 18 and 19. In F2 rats, there was a significant reduction in body weights of the BM-treated group at PND 1 as well as delayed onset of puberty, and decreases in FSH levels, Leydig cell volume, sperm number and motility, seminal vesicle contractility and ejaculated volume...
May 10, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28500826/serum-kisspeptin-concentrations-are-negatively-correlated-with-body-mass-index-in-adolescents-with-anorexia-nervosa-and-amenorrhea
#17
Flora Bacopoulou, George I Lambrou, Maria-Evangelia Rodanaki, Evgenia Stergioti, Vasiliki Efthymiou, Efthymios Deligeoroglou, Sophia I Markantonis
OBJECTIVE: To measure, for the first time, serum kisspeptin concentrations in adolescent females with anorexia nervosa (AN) and associated amenorrhea, and investigate potential correlations of kisspeptin with anthropometric, bone and hormonal data. DESIGN: Setting: University Adolescent Medicine Center. PARTICIPANTS: Females aged 12-20 years with typical or atypical AN (based on DSM-5 diagnostic criteria) and controls. INTERVENTIONS: Measurement of body mass index (BMI), whole body/lumbar spine bone mineral density and serum concentrations of kisspeptin, follicle stimulating hormone, luteinizing hormone (LH), prolactin (PRL), thyroid stimulating hormone (TSH), free thyroxine, triiodothyronine, estradiol (E2), 17-hydroxyprogesterone...
January 2017: Hormones: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28500250/characterization-of-nmb-grp-and-their-receptors-brs3-nmbr-and-grpr-in-chickens
#18
Chunheng Mo, Long Huang, Lin Cui, Can Lv, Dongliang Lin, Liang Song, Guoqiang Zhu, Juan Li, Yajun Wang
The two structurally and functionally related peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) play critical roles in many physiological/pathological processes in mammals. However, the information regarding the expression and functionality of avian NMB, GRP, and their receptors is limited. Here, we characterized cNMB, cGRP and their receptors (cNMBR, cGRPR, cBRS3) in chickens. Our results showed that: 1) cNMBR and cGRPR expressed in CHO cells could be potently activated by cNMB and cGRP respectively, as monitored by cell-based luciferase reporter assays, indicating that cNMBR and cGRPR are cNMB- and cGRP-specific receptors; Strikingly, BRS3 of chickens (/spotted gars), which is orthologous to mouse bombesin receptor subtype-3 (BRS3), could be potently activated by GRP and NMB, demonstrating that both peptides are the endogenous ligands for chicken(/spotted gar) BRS3; 2) Quantitative real-time PCR revealed that cGRPR is widely expressed in chicken tissues with abundant expression in the ovary, pancreas, proventriculus, spinal cord and brain, whereas cNMB, cNMBR and cBRS3 are mainly expressed in the brain and testes; 3) Interestingly, qPCR, Western blot, and immuno-staining revealed that cGRP is predominantly expressed in the anterior pituitary and mainly localized to LH-cells, suggesting that cGRP is likely a novel pituitary hormone in chickens...
May 12, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/28498971/deletion-of-rhoa-in-progesterone-receptor-expressing-cells-leads-to-luteal-insufficiency-and-infertility-in-female-mice
#19
Ahmed E El Zowalaty, Rong Li, Yi Zheng, John P Lydon, Francesco J DeMayo, Xiaoqin Ye
RhoA is widely expressed throughout the female reproductive system. To assess its role in progesterone receptor (Pgr) expressing cells, we generated RhoA conditional knockout mice RhoAd/d (RhoAf/f-Pgr-Cre+/-). RhoAd/d females had comparable mating activity, serum LH, prolactin, and estradiol levels and ovulation with control but were infertile with progesterone insufficiency, indicating impaired steroidogenesis in RhoAd/d corpus luteum (CL). RhoA was highly expressed in wild type luteal cells and conditionally deleted in RhoAd/d CL...
May 11, 2017: Endocrinology
https://www.readbyqxmd.com/read/28495475/ameliorative-effects-of-achillea-millefolium-inflorescences-alcoholic-extract-against-nicotine-induced-reproductive-failure-in-rat
#20
M Hallaj Salahipour, Sh Hasanzadeh, H Malekinejad
BACKGROUND: Nicotine (Nic) is a major risk factor in the development of functional disorders of male reproductive system. Achillea millefolium; is highly regarded for medicinal activities, due to its antioxidant and anti-inflammatory properties. This study was carried out to evaluate whether Achillea millefolium (Achm) inflorescences alcoholic extract could serve as a protective agent in male reproductive male failures during Nic exposure in a rat model. METHODS: Twenty-five adult male Wistar rats were categorized into the five groups...
May 8, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
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