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Christoph W Turck, Christian Webhofer, Markus Nussbaumer, Larysa Teplytska, Alon Chen, Giuseppina Maccarrone, Michaela D Filiou
PURPOSE: In this work, we discuss how in vivo (15) N metabolic labeling in combination with MS simultaneously provides information on protein expression and protein turnover. EXPERIMENTAL DESIGN: We metabolically labeled mice with the stable nitrogen isotope (15) N using a (15) N-enriched diet and analyzed unlabeled ((14) N) versus (15) N-labeled brain tissue with LC-MS/MS. We then compared the (14) N versus (15) N peptide isotopologue clusters of (14) N and (15) N-labeled dihydropyrimidinase-related (DPYSL) proteins...
December 2016: Proteomics. Clinical Applications
Rajesh Ponnusamy, Andrey A Lebedev, Steffen Pahlow, Bernhard Lohkamp
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins that are mainly involved in neuronal cell development. In humans, the CRMP family comprises five members. Here, crystal structures of human CRMP-4 in a truncated and a full-length version are presented. The latter was determined from two types of crystals, which were either twinned or partially disordered. The crystal disorder was coupled with translational NCS in ordered domains and manifested itself with a rather sophisticated modulation of intensities...
June 2014: Acta Crystallographica. Section D, Biological Crystallography
Fei Tan, Reema Wahdan-Alaswad, Shuang Yan, Carol J Thiele, Zhijie Li
Dihydropyrimidinase-like proteins (DPYSLs) are a family of proteins developmentally regulated during maturation of the nervous system. Recently, members of the DPYSL family have been reported to be involved in cancer with low expression of DPYSL1 correlating with poor clinical outcomes in non-small cell lung cancer and functioning as a metastasis suppressor. Neuroblastoma (NB) is a tumor derived from precursor cells of the sympathetic nervous system and is the most common solid tumor in childhood. So far the biological functions of DPYSLs in NB remain elusive...
December 2013: Cancer Science
Rii Morimura, Keisuke Nozawa, Hideomi Tanaka, Toshio Ohshima
Dpysls (CRMPs) that were initially identified as mediator proteins of Semaphorin3a (Sema3a) signaling are involved in neuronal polarity and axon elongation in cultured neurons. Previous studies have shown that knockdown of neuropilin1a, one of the sema3a receptors, exhibited ectopic primary motor neurons (PMNs) outside of the spinal cord in zebrafish. However, downstream molecules of sema3a signaling involved in the positioning of motor neurons are largely unknown. Here, we addressed the role of Dpysl2 (CRMP2) and Dpysl3 (CRMP4) in the positioning of PMNs in the zebrafish spinal cord...
December 2013: Developmental Neurobiology
Junko Kimura, Takuya Kudoh, Yoshio Miki, Kiyotsugu Yoshida
The p53 tumor suppressor gene, which is frequently mutated in a wide variety of tumors, plays an important role in maintaining genomic integrity. Following genotoxic insults, the protein level of p53 is increased, and p53 functions as a sequence-specific transcription factor that regulates the expression of downstream target genes required for cell cycle arrest, DNA repair or apoptosis. However, the mechanism for p53-inducible apoptosis remains largely unclear. To search novel downstream targets of p53 on apoptosis, we had carried out microarray analysis...
April 1, 2011: International Journal of Cancer. Journal International du Cancer
Balu Chakravarthy, Amal Rashid, Leslie Brown, Luc Tessier, John Kelly, Michel Ménard
Gap-43 (B-50, neuromodulin) is a presynaptic protein implicated in axonal growth, neuronal differentiation, plasticity, and regeneration. Its activities are regulated by its dynamic interactions with various neuronal proteins, including actin and brain spectrin. Recently we have shown that Gap-43 co-localizes with an axonal protein DPYSL-3 in primary cortical neurons. In the present study we provide evidence that Gap-43 co-localizes and potentially interacts with microtubule-associated protein MAP-2 in adult and fetal rat brain, as well as in primary neuronal cultures...
July 11, 2008: Biochemical and Biophysical Research Communications
H Ujike, A Sakai, K Nakata, Y Tanaka, T Kodaka, Y Okahisa, M Harano, T Inada, M Yamada, T Komiyama, T Hori, Y Sekine, N Iwata, I Sora, M Iyo, N Ozaki, S Kuroda
Dihydropyrimidinase-related protein 2 (DRP-2 or DPYSL-2)mediates the intracellular response to collapsin, a repulsive extracellular guidance cue or axonal outgrowth. DRP-2 is also referred to as collapsin response mediator protein 2 (CRMP-2). We have previously demonstrated that the DRP-2 gene is associated with susceptibility to schizophrenia, but not to bipolar disorders. In addition, a genetic association was observed with paranoid-type schizophrenia, but not with hebephrenic-type schizophrenia. It has been well documented that repeated abuse of methamphetamine (METH) for a long period frequently produces psychotic symptoms, such as auditory hallucinations and delusions that are hardly distinguishable from those of paranoid-type schizophrenia...
August 2006: Annals of the New York Academy of Sciences
Jennifer J Hill, Deborah A Callaghan, Wen Ding, John F Kelly, Balu R Chakravarthy
Okadaic acid (OA) is a widely used small-molecule phosphatase inhibitor that is thought to selectively inhibit protein phosphatase 2A (PP2A). Multiple studies have demonstrated that PP2A activity is compromised in the brains of Alzheimer's disease patients. Thus, we set out to determine changes in phosphorylation that occur upon OA treatment of neuronal cells. Utilizing isotope-coded affinity tags and mass spectrometry analysis, we determined the relative abundance of proteins in a phosphoprotein enriched fraction from control and OA-treated primary cortical neurons...
April 14, 2006: Biochemical and Biophysical Research Communications
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