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https://www.readbyqxmd.com/read/28651331/il-1%C3%AE-up-regulates-star-and-progesterone-production-through-the-erk1-2-and-p38-mediated-creb-signaling-pathways-in-human-granulosa-lutein-cells
#1
Xuan Dang, Qinling Zhu, Yaqiong He, Yuan Wang, Yao Lu, Xiaoxue Li, Jia Qi, Hasiximuke Wu, Yun Sun
The pro-inflammatory cytokine interleukin-1β (IL-1β) may be involved in several ovulation-associated events, such as proteases synthesis, prostaglandin production and steroidogenesis in granulosa cells. However, the exact effect of IL-1β on progesterone synthesis in granulosa cells and the underlying mechanism remain unclear. By using cultured granulosa-lutein cells collected from women undergoing in vitro fertilization or intracytoplasmic sperm injection, we found that IL-1β up-regulated steroidogenic acute regulatory protein (StAR) expression and progesterone synthesis in granulosa-lutein cells, which was comparable with luteinizing hormone (LH) effect and could be abolished by an IL-1 receptor antagonist...
June 23, 2017: Endocrinology
https://www.readbyqxmd.com/read/28649090/mirna-143-mediates-the-proliferative-signaling-pathway-of-fsh-and-regulates-estradiol-production
#2
Li Zhang, XiaoXin Zhang, Xuejing Zhang, Yu Lu, Lei Li, Sheng Cui
MicroRNAs (MiRNAs) play important regulatory roles in many cellular processes. MiR-143 is highly enriched in the mouse ovary, but its roles and underlying mechanisms are not well understood. In the current study, we show that miR-143 is located in granulosa cells of primary, secondary and antral follicles. To explore the specific functions of miR-143, we transfected miR-143 inhibitor into primary cultured granulosa cells to study the loss of function of miR-143 and the results showed that miR-143 silencing significantly increased estradiol production and steroidogenesis-related gene expression...
July 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28645283/oocyte-polarized-light-microscopy-assay-of-specific-follicular-fluid-metabolites-and-gene-expression-in-cumulus-cells-as-different-approaches-to-predict-fertilization-efficiency-after-icsi
#3
Alberto Revelli, Stefano Canosa, Loredana Bergandi, Oleksii A Skorokhod, Valentina Biasoni, Andrea Carosso, Angela Bertagna, Milena Maule, Elisabetta Aldieri, Maria Diletta D'Eufemia, Francesca Evangelista, Nicola Colacurci, Chiara Benedetto
BACKGROUND: The complex relationship between oocyte morphology, specific follicular fluid metabolites, gene expression in cumulus granulosa cells, and oocyte competence toward fertilization and embryo development still needs further clarification. METHODS: Forty-six oocytes retrieved from the largest pre-ovulatory follicle of patients undergoing intra-cytoplasmic sperm injection (ICSI) were considered assessing: (a) oocyte morphological characteristics at polarized light microscopy (PLM), (b) specific follicular fluid (FF) metabolites previously suggested to influence oocyte competence (AMH, markers of redox status and of cytotoxicity), (c) transcription of AMH and AMH type II receptor genes in cumulus cells...
June 23, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28644873/circular-rna-expression-profiling-of-human-granulosa-cells-during-maternal-aging-reveals-novel-transcripts-associated-with-assisted-reproductive-technology-outcomes
#4
Jing Cheng, Jia Huang, Suzhen Yuan, Su Zhou, Wei Yan, Wei Shen, Yun Chen, Xi Xia, Aiyue Luo, Da Zhu, Shixuan Wang
Circular RNAs (circRNAs) are a unique class of endogenous RNAs which could be used as potential diagnostic and prognostic biomarkers of many diseases. Our study aimed to investigate circRNA profiles in human granulosa cells (GCs) during maternal aging and to uncover age-related circRNA variations that potentially reflect decreased oocyte competence. CircRNAs in GCs from in vitro fertilization (IVF) patients with young age (YA, ≤ 30 years) and advanced age (AA, ≥ 38 years) were profiled by microarray, and validated in 20 paired samples...
2017: PloS One
https://www.readbyqxmd.com/read/28643774/insufficient-antibody-validation-challenges-oestrogen-receptor-beta-research
#5
Sandra Andersson, Mårten Sundberg, Nusa Pristovsek, Ahmed Ibrahim, Philip Jonsson, Borbala Katona, Carl-Magnus Clausson, Agata Zieba, Margareta Ramström, Ola Söderberg, Cecilia Williams, Anna Asplund
The discovery of oestrogen receptor β (ERβ/ESR2) was a landmark discovery. Its reported expression and homology with breast cancer pharmacological target ERα (ESR1) raised hopes for improved endocrine therapies. After 20 years of intense research, this has not materialized. We here perform a rigorous validation of 13 anti-ERβ antibodies, using well-characterized controls and a panel of validation methods. We conclude that only one antibody, the rarely used monoclonal PPZ0506, specifically targets ERβ in immunohistochemistry...
June 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28637499/diagnostic-relevance-of-urinary-steroid-profiles-on-ovarian-granulosa-cell-tumors-two-case-reports
#6
Anita Bufa, Nelli Farkas, Zsolt Preisz, Viktória Poór, Csilla Páger, Sándor Szukits, Bálint Farkas, Péter Miklós Gőcze
BACKGROUND: Granulosa cell tumor of the ovary is the most frequent sex cord stromal tumor and represents 2 to 5% of all primary ovarian cancers. Ovarian granulosa cell tumor is a malignant tumor with slow progression and in some cases this tumor is hormonally active. The recurrence of granulosa cell tumor often happens after 5 years. CASE PRESENTATION: We describe two cases of postmenopausal women with adult-type granulosa cell tumors of the ovary. Patient 1 is a 49-year-old European woman with a recurrent tumor; patient 2 is a 55-year-old European woman without recurrence of tumor...
June 22, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/28635583/cigarette-smoke-is-associated-with-altered-expression-of-antioxidant-enzymes-in-granulosa-cells-from-women-undergoing-in-vitro-fertilization
#7
Maria Cristina Budani, Erminia Carletti, Gian Mario Tiboni
This study was undertaken to evaluate whether cigarette smoke is associated with changes in the expression of antioxidant enzymes in granulosa cells of women undergoing IVF treatments. For this aim, the expression of three antioxidant enzymes (SOD1, SOD2 and catalase) in non-smokers (n = 20) and smokers (n = 20) was analyzed. There was a statistically significant overexpression of SOD2 and catalase mRNA levels in smokers in comparison with non-smokers. Cigarette smoking was associated with a lower fertilization rate, implantation rate and pregnancy rate in comparison with non-smokers...
June 21, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28633436/membrane-androgen-receptor-zip9-induces-croaker-ovarian-cell-apoptosis-via-stimulatory-g-protein-alpha-subunit-and-mapkinase-signaling
#8
Aubrey Converse, Chenan Zhang, Peter Thomas
Recent studies show that androgen-induced apoptosis in Atlantic croaker primary granulosa/theca (G/T) cells and in human breast and prostate cancer cell lines is mediated by the novel membrane androgen receptor ZIP9 which belongs to the SLC39A zinc transporter family. However, the apoptotic signaling pathways remain unclear because ZIP9 activates an inhibitory G protein in human cancer cells, whereas recombinant croaker ZIP9 activates a stimulatory G protein (Gs) in transfected cancer cells. Here we investigated androgen-dependent apoptotic pathways in order to identify the signaling pathways regulated through wild-type croaker ZIP9 in ovarian follicle cells...
June 16, 2017: Endocrinology
https://www.readbyqxmd.com/read/28629378/microrna-130b-is-involved-in-bovine-granulosa-and-cumulus-cells-function-oocyte-maturation-and-blastocyst-formation
#9
Pritam Bala Sinha, Dawit Tesfaye, Franca Rings, Munir Hossien, Michael Hoelker, Eva Held, Christaine Neuhoff, Ernst Tholen, Karl Schellander, Dessie Salilew-Wondim
BACKGROUND: Oocyte maturation and preimplantation embryo development are controlled by array of genes that are post-transcriptionally regulated by microRNAs. With respect to this, previously, we identified altered expression of microRNA-130b (miR-130b) during oocyte maturation. Here, we aimed to investigate the role of miR-130b in bovine granulosa and cumulus cell function, oocyte maturation and preimplantation embryo development using gain- and loss-of- function approach. METHODS: For this study, the granulosa cells, cumulus cells and the oocytes were collected from ovaries obtained from slaughterhouse...
June 19, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28626751/feeder-cell-type-affects-the-growth-of-in-vitro-cultured-bovine-trophoblast-cells
#10
Islam M Saadeldin, Ahmed Abdelfattah-Hassan, Ayman Abdel-Aziz Swelum
Trophectoderm cells are the foremost embryonic cells to differentiate with prospective stem-cell properties. In the current study, we aimed at improving the current approach for trophoblast culture by using granulosa cells as feeders. Porcine granulosa cells (PGCs) compared to the conventional mouse embryonic fibroblasts (MEFs) were used to grow trophectoderm cells from hatched bovine blastocysts. Isolated trophectoderm cells were monitored and displayed characteristic epithelial/cuboidal morphology. The isolated trophectoderm cells expressed mRNA of homeobox protein (CDX2), cytokeratin-8 (KRT8), and interferon tau (IFNT)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28626555/live-imaging-of-follicle-stimulating-hormone-receptors-in-gonads-and-bones-using-near-infrared-ii-fluorophore
#11
Yi Feng, Shoujun Zhu, Alexander L Antaris, Hao Chen, Yuling Xiao, Xiaowei Lu, Linlin Jiang, Shuo Diao, Kuai Yu, Yan Wang, Sonia Herraiz, Jingying Yue, Xuechuan Hong, Guosong Hong, Zhen Cheng, Hongjie Dai, Aaron J Hsueh
In vivo imaging of hormone receptors provides the opportunity to visualize target tissues under hormonal control in live animals. Detecting longer-wavelength photons in the second near-infrared window (NIR-II, 1000-1700 nm) region affords reduced photon scattering in tissues accompanied by lower autofluorescence, leading to higher spatial resolution at up to centimeter tissue penetration depths. Here, we report the conjugation of a small molecular NIR-II fluorophore CH1055 to a follicle stimulating hormone (FSH-CH) for imaging ovaries and testes in live mice...
May 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28626448/the-common-follicle-stimulating-hormone-receptor-fshr-promoter-polymorphism-fshr-29g%C3%A2-%C3%A2-a-affects-androgen-production-in-normal-human-small-antral-follicles
#12
Tanni Borgbo, Hana Klučková, Milan Macek, Jana Chrudimska, Stine Gry Kristensen, Lise Lotte Hansen, Claus Yding Andersen
Follicle-stimulating hormone receptors (FSHRs) are almost exclusively expressed on granulosa cells, and FSH action is probably most clearly reflected in intrafollicular hormone milieu of antral follicles. Little is known about the possible effects of the common single nucleotide polymorphism (SNP) FSHR -29G > A (rs1394205) on hormonal conditions in humsan small antral follicles (hSAFs) obtained from women in the natural menstrual cycle. This study investigated the follicle fluid (FF) concentrations of anti-Müllerian hormone, estradiol, progesterone, androstenedione, and testosterone in hSAF in relation to the different genotypes of FSHR -29G > A...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28624767/effects-of-nrf1-on-steroidogenesis-and-apoptosis-in-goat-luteinized-granulosa-cells
#13
Guo-Min Zhang, Ming-Tian Deng, Zhi-Hai Lei, Yong-Jie Wan, Hai-Tao Nie, Zi-Yu Wang, Yi-Xuan Fan, Feng Wang, Yan-Li Zhang
During goat follicular development, abnormal expression of nuclear respiratory factor 1 (NRF1) in granulosa cells may drive follicular atresia with unknown regulatory mechanisms. In this study, we investigated the effects of NRF1 on steroidogenesis and cell apoptosis by overexpressing or silencing it in goat luteinized granulosa cells (LGCs). Results showed that knockdown of NRF1 expression significantly inhibited the expression of STAR and CYP19A1, which are involved in sex steroid hormones synthesis, and led to lower estrogen levels...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28624766/dhea-protects-mitochondria-against-dual-modes-of-apoptosis-and-necroptosis-in-human-granulosa-ho23-cells
#14
Kuan-Hao Tsui, Peng-Hui Wang, Li-Te Lin, Chia-Jung Li
Because ovarian granulosa cells are essential for oocyte maturation and development, we validated human granulosa HO23 cells to evaluate the ability of the DHEA to prevent cell death after starvation. The present study was aimed to investigate whether DHEA could protect against starvation-induced apoptosis and necroptosis in human oocyte granulosa HO23 cells. The starvation was induced by treatment of serum-free (SF) medium for 4 h in vitro Starvation-induced mitochondrial depolarization, cytochrome c release and caspase-3 activation were largely prevented by DHEA in HO23 cells...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28621049/lower-foxo3-mrna-expression-in-granulosa-cells-is-involved-in-unexplained-infertility
#15
Hikaru Yamamoto, Yoshiki Yamashita, Natsuho Saito, Atsushi Hayashi, Masami Hayashi, Yoshito Terai, Masahide Ohmichi
AIM: The aim of this study was to investigate whether FOXO1 and FOXO3 mRNA expression in granulosa cells is the cause of unexplained infertility. METHODS: Thirty-one patients aged <40 years (13 with unexplained infertility and 18 with male partner infertility as a control group) whose serum anti-Müllerian hormone level was >0.5 ng/μL were enrolled in the study. All patients underwent oocyte retrieval under a short protocol from June 2012 to October 2013...
June 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28613046/anti-mullerian-hormone-amh-determinations-in-the-pediatric-and-adolescent-endocrine-practice
#16
Amir Weintraub, Talia Eldar-Geva
Anti-Mullerian hormone (AMH), secreted by immature testicular Sertoli-cells, triggers the regression of male fetal Mullerian ducts. During puberty, AMH is downregulated by intratesticular testosterone. In females, AMH is secreted from granulosa cells of immature ovarian follicles from late prenatal life until menopause; serum concentration is 5-20 times lower in females than in males through lifetime. In boys, AMH determination is useful in the clinical setting as a marker of Sertoli cell function. Serum AMH is low in infants with hypogonadotrophic hypogonadism (and increases with FSH treatment), in patients with primary hypogonadism from early postnatal life and in Klinefelter syndrome from midpuberty...
June 2017: Pediatric Endocrinology Reviews: PER
https://www.readbyqxmd.com/read/28609289/adverse-eff-ects-of-polymeric-nanoparticle-poly-ethylene-glycol-block-polylactide-methyl-ether-peg-b-pla-on-steroid-hormone-secretion-by-porcine-granulosa-cells
#17
Sona Scsukova, Mlynarcikova A Bujnakova, A Kiss, E Rollerova
OBJECTIVES: Development of nanoparticles (NPs) for biomedical applications, including medical imaging and drug delivery, is currently undergoing a dramatic expansion. Diverse effects of different type NPs relating to mammalian reproductive tissues have been demonstrated. Th e objective of this study was to explore the in vitro effects of polymeric nanoparticle poly(ethylene glycol)-blockpolylactide methyl ether (PEG-b-PLA NPs) on functional state and viability of ovarian granulosa cells (GCs), which play an important role in maintaining ovarian function and female fertility...
April 25, 2017: Endocrine Regulations
https://www.readbyqxmd.com/read/28607932/the-protective-effect-of-n-acetylcysteine-on-ionizing-radiation-induced-ovarian-failure-and-loss-of-ovarian-reserve-in-female-mouse
#18
Wei Gao, Jin-Xiao Liang, Chi Ma, Jing-Yin Dong, Qiu Yan
Ionizing radiation may cause irreversible ovarian failure, which, therefore, calls for an effective radioprotective reagent. The aim of the present study was to evaluate the potential radioprotective effect of N-acetylcysteine (NAC) on ionizing radiation induced ovarian failure and loss of ovarian reserve in mice. Kun-Ming mice were either exposed to X-irradiation (4 Gy), once, and/or treated with NAC (300 mg/kg), once daily for 7 days before X-irradiation. We examined the serum circulating hormone levels and the development of ovarian follicles as well as apoptosis, cell proliferation, and oxidative stress 24 hours after X-irradiation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28607476/zinc-finger-gene-217-znf217-promoted-ovarian-hyperstimulation-syndrome-ohss-through-regulating-e2-synthesis-and-inhibiting-thrombospondin-1-tsp-1
#19
Junyu Zhai, Jiansheng Liu, Xiaoyue Cheng, Shang Li, Yan Hong, Kang Sun, Zi-Jiang Chen, Yanzhi Du, Weiping Li
Zinc finger gene 217 (ZNF217) is a candidate gene of polycystic ovary syndrome (PCOS) which is vulnerable to ovarian hyperstimulation syndrome (OHSS). However, the relationship between ZNF217 and OHSS is largely unknown. Our study demonstrated that ZNF217 was mainly distributed in the granulosa cells of rat ovary. Significantly higher expression of ovarian ZNF217 was detected in OHSS rats, being consistent with serum 17β-estradiol concentration and ovarian aromatase. Moreover, OHSS rats also showed decreased ovarian TSP-1 mRNA, an acknowledged VEGF signaling suppressor...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28606727/expression-patterns-of-p38%C3%AE-mapk-during-follicular-development-in-the-ovaries-of-neonatal-rats
#20
Shifu Hu, Meng Rao, Hui Lei, Yanqing Wu, Yingying Wang, Dandan Ke, Wei Xia, Changhong Zhu
The p38αMAPK signaling pathway plays a critical role in female reproduction, but an understanding of its expression in rats remains elusive. This study was carried out to investigate the temporal and spatial expression of p38αMAPK and p-p38αMAPK. Ovarian tissue samples were collected from 2-, 4-, 8-, 12-, 16-, 20- and 30-day-old female rats. Western blotting was used to examine the relative expression of p38αMAPK and p-p38αMAPK in ovarian tissue, and subcellular localization was examined using immunohistochemistry of the rat ovaries at different ages of postpartum...
June 10, 2017: Acta Histochemica
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