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https://www.readbyqxmd.com/read/29155477/oxidized-low-density-lipoprotein-suppresses-mouse-granulosa-cell-differentiation-through-disruption-of-the-hypoxia-inducible-factor-1-pathway
#1
Xiaoliang Chen, Yanhong Liu, Yanhong Shan, Xingyi Jin, Qingyang Shi, Chunshu Jia
Obesity predisposes women to reproductive disorders. One symptom of obesity in women is higher levels of oxidized Low-density lipoprotein (oxLDL) in serum and preovulatory follicles. The present study was designed to test the hypothesis that oxLDL might impair follicle differentiation and luteinization. Given that Hypoxia-inducible factor 1 (HIF1) plays crucial roles in supporting follicle differentiation and luteinization in mammals, we focused on oxLDL-mediated events that may affect the HIF1 pathway. We report that exposure to oxLDL diminished the expression of HIF1α and its target genes and suppressed the differentiation of mouse luteinized granulosa cells following induction by human Chorionic gonadotophin (hCG) under hypoxic conditions (1% oxygen)...
November 20, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29154873/medaka-igf1-identifies-somatic-cells-and-meiotic-germ-cells-of-both-sexes
#2
Cancan Yuan, Kerang Chen, Yefei Zhu, Yongming Yuan, Mingyou Li
Insulin-like growth factors (IGFs) play significant roles in regulating cell proliferation, differentiation and reproduction in diverse organisms. Here, we report the identification of medaka igf1 and the expression pattern of igf1 RNA in adult medaka gonads. Using RT-PCR, igf1 RNA was easily detected in several somatic organs and adult gonads of both sexes. Using chromogenic in situ hybridization, igf1 RNA in the ovary was found in stage I-IV oocytes and in the somatic cells throughout oogenesis. In the testis, igf1 RNA was present in meiotic products and the somatic cells surrounding male germ cells throughout spermatogenesis...
November 14, 2017: Gene
https://www.readbyqxmd.com/read/29151191/silica-nanoparticle-exposure-inducing-granulosa-cell-apoptosis-and-follicular-atresia-in-female-balb-c-mice
#3
Jianhui Liu, Man Yang, Li Jing, Lihua Ren, Jialiu Wei, Jin Zhang, Feng Zhang, Junchao Duan, Xianqing Zhou, Zhiwei Sun
Given that the effects of ultrafine fractions (< 0.1 μm) on reproductive diseases are gaining attention, this study aimed to explore the influence of silica nanoparticle (SiNP)-induced female reproductive dysfunction. In this study, 80 female mice were randomly divided into four groups including a control group and three concentrations of SiNP groups (7, 21, 35 mg/kg). Mice were exposed to the vehicle control and silica nanoparticles by tracheal perfusion every 3 days a total of five times in 15 days...
November 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29150243/effects-of-n-carbamylglutamate-and-l-arginine-on-steroidogenesis-and-gene-expression-in-bovine-granulosa-cells
#4
T Feng, L F Schütz, B C Morrell, M C Perego, L J Spicer
Feeding N-carbamylglutamate (NCG) and arginine (ARG) improves reproductive measures in pigs and reduces systemic steroid levels in pregnant ewes. We hypothesized that the effects of NCG and ARG on reproduction were due to direct effects on the ovary. Thus, the objectives of this study were to investigate the effects of NCG and ARG on granulosa cell (GC) steroidogenesis, gene expression, and cell proliferation in vitro. GC were collected from small (1-5mm) bovine follicles and treated in vitro with NCG or ARG in serum-free medium for 24h to 48h...
November 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29146461/resveratrol-supports-and-alpha-naphthoflavone-disrupts-growth-of-human-ovarian-follicles-in-an-in-vitro-tissue-culture-model
#5
Jie Hao, Astrud R Tuck, Marcus O D Sjödin, Johan Lindberg, Anna Sand, Boel Niklasson, Maria Argyraki, Outi Hovatta, Pauliina Damdimopoulou
Infertility is a global health problem with an estimated incidence of 15%. Exposure to chemicals is a potential causal factor, and there is a lack of studies examining the effects on female germ cells. Here, we have studied the impact of different aryl hydrocarbon receptor (AHR) modulators on human ovarian follicles using a human ovarian tissue culture model. Expression of AHR was analyzed in tissue samples, and effects of the selected ligands resveratrol (RSVL), 6-formylindolo(3,2-b)carbazole (FICZ), and alpha-naphthoflavone (aNF) on AHR transactivation studied in a granulosa cell tumor line...
November 13, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29140884/ovarian-sex-cord-stromal-tumors-with-melanin-pigment-report-of-a-previously-undescribed-phenomenon
#6
Jennifer Taylor, W Glenn McCluggage
We report 2 ovarian sex cord-stromal tumors, a luteinized adult granulosa cell tumor and a cellular fibroma, with melanin pigment. These occurred in 44 and 61-yr-old patients, respectively. As far as we are aware, melanin pigment has not been described previously in an ovarian sex cord-stromal tumor, although it has been reported in a testicular Sertoli cell tumor. We review ovarian neoplasms containing melanin pigment.
November 14, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/29138526/stimulation-of-primordial-follicle-assembly-by-estradiol-17%C3%AE-requires-the-action-of-bone-morphogenetic-protein-2-bmp2
#7
Prabuddha Chakraborty, Shyamal K Roy
Primordial follicle (PF) pool determines the availability of follicles for ovulation in all mammals. Premature depletion of the PF reserve leads to subfertility or infertility. Bone morphogenetic protein 2 (BMP2) promotes PF formation by facilitating oocyte and granulosa cell development. Estradiol-17β (E2) upregulates PF formation in developing hamster ovaries. However, if BMP2 mediates E2 effect is not known. We hypothesize that E2 facilitates the effect of BMP2 on somatic to granulosa cell transition. BMP2 and E2 together significantly upregulated the percentage of PFs in hamster fetal ovaries in vitro compared with either of the treatments alone...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29132723/ovarian-sex-cord-stromal-tumours-and-their-mimics
#8
REVIEW
Robert H Young
Sex cord-stromal tumours of the ovary include many of the most morphologically intriguing ovarian neoplasms and albeit many of them are rare, they factor into the differential diagnosis more often than their frequency might suggest. The most common malignant form, the adult granulosa cell tumour, may grossly simulate various surface epithelial neoplasms. Microscopically, confusion with endometrioid carcinoma may occur because the cords and microfollicles of the granulosa cell tumour may be mimicked by endometrioid carcinoma and the latter may have pale nuclei with nuclear grooves...
November 10, 2017: Pathology
https://www.readbyqxmd.com/read/29132131/il-6-promotes-fsh-induced-vegf-expression-through-jak-stat3-signaling-pathway-in-bovine-granulosa-cells
#9
Meng Yang, Lei Wang, Xurong Wang, Xuezhi Wang, Zhiqiang Yang, Jianxi Li
BACKGROUND/AIMS: Vascular endothelial growth factor (VEGF) has been demonstrated to play a pivotal role in the regulation of angiogenesis in ovarian follicular development, particularly during the preovulatory period. Although numerous studies have shown that interleukin-6 (IL-6) is one of the major inducing factors that regulate the expression of VEGF in non-ovarian cells, whether it involved in regulating the expression of VEGF in normal ovarian granulosa cells is still unknown. The aim of this study was to elucidate the mechanisms underlying the effect of IL-6 on FSH-induced VEGF expression in bovine granulosa cells derived from large follicles...
November 13, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29132039/the-cxcl12-cxcr4-signaling-promotes-oocyte-maturation-by-regulating-cumulus-expansion-in-sheep
#10
Rui-Na Zhang, Bo Pang, Shang-Rong Xu, Peng-Cheng Wan, Song-Chang Guo, Han-Zhong Ji, Gong-Xue Jia, Lin-Yong Hu, Xin-Quan Zhao, Qi-En Yang
Gonadotropins and growth factors synergistically regulate folliculogenesis and oocyte development. C-X-C motif chemokine 12 (CXCL12) and its receptor CXCR4 are expressed in ovaries of sheep, cattle and other species, however, roles of this multifunctional signal axis in oocyte maturation are not defined. Using sheep as a model, we examined the expression patterns and functions of the CXCL12-CXCR4 axis during oocyte maturation. CXCL12 and CXCR4 mRNA and protein were present in oocytes and granulosa cells. Relative abundance of CXCR4 transcript was controlled by epidermal growth factor (EGF)...
November 4, 2017: Theriogenology
https://www.readbyqxmd.com/read/29131373/differentially-expressed-mirna-210-during-follicular-luteal-transition-regulates-pre-ovulatory-granulosa-cell-function-targeting-hras-and-efna3
#11
Astha Shukla, Sunita Dahiya, Suneel Kumar Onteru, Dheer Singh
Ovarian folliculogenesis, ovulation and luteinization are an important prerequisite for fertility performance in mammals. Spatial and temporal key factors and proteins for their regulation are well known. Recent advancement in the field of molecular biology led to the discovery of another class of gene regulators, microRNA (miRNA). Previous studies on profiling of miRNA in buffalo ovaries revealed that miRNA-210 (miR-210) is differently expressed in follicular-luteal transition. Therefore, the present study was planned to ascertain the role of miR-210 in buffalo granulosa cells...
November 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29130964/changes-in-the-proliferation-apoptosis-balance-in-the-bovine-ovary-a-key-early-event-in-follicular-persistence
#12
E Matías Belotti, Antonela F Stassi, Melisa M L Velázquez, Pablo U Díaz, Belkis E Marelli, Florencia Rey, Ulises S Notaro, Hugo H Ortega, Natalia R Salvetti
The objective of this work was to evaluate proliferation and apoptosis in the bovine ovary in a model of follicular persistence induced by low levels of progesterone to detect incipient changes during cystic ovarian disease development on the expected day of ovulation (day 0) and after 5, 10, and 15 days of follicular persistence. We analyzed cell proliferation by evaluating the expression of Ki-67 and apoptosis by evaluating caspase-3, BAX, and BCL2 expression. Proliferation was similar in the granulosa and theca cells of antral follicles in the P0 group (treated with progesterone up to the expected day of ovulation) and in the control group...
November 3, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29129645/incretins-modulate-progesterone-biosynthesis-by-regulating-bone-morphogenetic-protein-activity-in-rat-granulosa-cells
#13
Yuki Nishiyama, Toru Hasegawa, Shiho Fujita, Nahoko Iwata, Satoko Nagao, Takeshi Hosoya, Kenichi Inagaki, Jun Wada, Fumio Otsuka
The effects of incretins on ovarian steroidogenesis have not been clarified. In this study, we investigated the effects of incretins, including GIP and GLP-1, on ovarian steroidogenesis using rat primary granulosa cells. Treatment with incretins significantly suppressed progesterone synthesis in the presence of FSH, and the effect of GIP was more potent than that of GLP-1. In contrast, incretins had no significant effect on estrogen synthesis by rat granulosa cells. In accordance with the effects of incretins on steroidogenesis, GIP and GLP-1 suppressed the expression of progesterogenic factors and enzymes, including StAR, P450scc, 3βHSD, but not P450arom, and cellular cAMP synthesis induced by FSH...
November 9, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29126831/characterization-of-the-ovarian-preantral-follicle-populations-and-its-correlation-with-age-and-nutritional-status-in-brazilian-northeastern-donkeys-equus-assinus
#14
K R F Lopes, G L Lima, L G P Bezerra, R A Barreto-Junior, M F Oliveira, A R Silva
This study aimed to characterize the ovarian follicular population and to determine its correlations with the age and nutritional status of donkeys of the Northeastern Brazilian breed. A total of 10 females with a mean age of 5.1±3.2years were submitted to ovariectomy by videolaparoscopy to obtain the ovaries. In the laboratory, the ovaries were sectioned into 6-12 fragments of approximately 7mm in diameter, which were fixed in Carnoy, dehydrated in increasing concentrations of alcohol (85%, 95% and absolute), clarified using xylol and embedded in blocks of histological paraffin...
November 7, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29126830/transcriptome-studies-of-granulosa-cells-at-different-stages-of-ovarian-follicular-development-in-buffalo
#15
J Li, Z Li, S Liu, R Zia, A Liang, L Yang
The normal maturation and ovulation from ovarian follicles is important in ensuring conception and improving fertility of buffalo. The molecular regulation mechanism of buffalo follicles growth, however, remains unknown. This study analyzed the gene expression profiles associated with buffalo ovarian follicle growth. According to the analysis of RNA sequencing, 17,700 unigenes and 13,672 differentially expressed genes (DEGs) were detected. A total of 30 common DEGs were identified during four stages of follicle growth, and the expression patterns are basically synchronized, suggesting the products as a result of expressions of these genes may cooperate to regulate follicular development...
November 6, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29126263/notch-signaling-regulates-differentiation-and-steroidogenesis-in-female-mouse-ovarian-granulosa-cells
#16
Rexxi D Prasasya, Kelly E Mayo
The Notch pathway is a highly conserved juxtacrine signaling mechanism that is important for many cellular processes during development, including differentiation and proliferation. While Notch is important during ovarian follicle formation and early development, its functions during the gonadotropin-dependent stages of follicle development are largely unexplored. We observed positive regulation of Notch activity and expression of Notch ligands and receptors following activation of the LH-receptor in prepubertal mouse ovary...
November 8, 2017: Endocrinology
https://www.readbyqxmd.com/read/29126187/med12-regulates-ovarian-steroidogenesis-uterine-development-and-maternal-effects-in-the-mammalian-egg
#17
Xinye Wang, Priya Mittal, Carlos A Castro, Gabriel Rajkovic, Aleksandar Rajkovic
The transcriptional factor MED12 is part of the essential mediator transcriptional complex that acts as a transcriptional coactivator in all eukaryotes. Missense gain of function mutations in human MED12 are associated with uterine leiomyomas, yet the role of MED12 deficiency in tumorigenesis and reproductive biology has not been fully explored. We generated a Med12 reproductive conditional knockout mouse model to evaluate its role in uterine mesenchyme, granulosa cells, and oocytes. Mice heterozygous for Med12 deficiency in granulosa cells and uterus (Med12fl/+ Amhr2-Cre) were subfertile, while mice homozygous for Med12 deficiency in granulosa cells and uterus (Med12fl/fl Amhr2-Cre) were infertile...
November 8, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29125859/insulin-resistance-enhances-the-mitogen-activated-protein-kinase-signaling-pathway-in-ovarian-granulosa-cells
#18
Linghui Kong, Qien Wang, Jiewen Jin, Zou Xiang, Taoyu Chen, Shanmei Shen, Hongwei Wang, Qian Gao, Yong Wang
The ovary is the main regulator of female fertility. Granulosa cell dysfunction may be involved in various reproductive endocrine disorders. Here we investigated the effect of insulin resistance on the metabolism and function of ovarian granulosa cells, and dissected the functional status of the mitogen-activated protein kinase signaling pathway in these cells. Our data showed that dexamethasone-induced insulin resistance in mouse granulosa cells reduced insulin sensitivity, accompanied with an increase in phosphorylation of p44/42 mitogen-activated protein kinase...
2017: PloS One
https://www.readbyqxmd.com/read/29124467/spathaspora-piracicabensis-f-a-sp-nov-a-d-xylose-fermenting-yeast-species-isolated-from-rotting-wood-in-brazil
#19
Camila S Varize, Raquel M Cadete, Lucas D Lopes, Renata M Christofoleti-Furlan, Marc-André Lachance, Carlos A Rosa, Luiz C Basso
Two strains of a novel yeast species were isolated from rotting wood of an ornamental tree (purple quaresmeira, Tibouchina granulosa, Melastomataceae) in an Atlantic Rainforest area in Brazil. Analysis of the sequences of the internal transcribed spacer (ITS-5.8S) region and the D1/D2 domains of the large subunit rRNA gene showed that this species belongs to the Spathaspora clade, and is phylogenetically related to Spathaspora brasiliensis, Candida materiae and Sp. girioi. The novel species ferments D-xylose, producing ethanol, with amounts between 3...
November 9, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29117219/cellular-and-exosome-mediated-molecular-defense-mechanism-in-bovine-granulosa-cells-exposed-to-oxidative-stress
#20
Mohammed Saeed-Zidane, Lea Linden, Dessie Salilew-Wondim, Eva Held, Christiane Neuhoff, Ernst Tholen, Michael Hoelker, Karl Schellander, Dawit Tesfaye
Various environmental insults including diseases, heat and oxidative stress could lead to abnormal growth, functions and apoptosis in granulosa cells during ovarian follicle growth and oocyte maturation. Despite the fact that cells exposed to oxidative stress are responding transcriptionally, the potential release of transcripts associated with oxidative stress response into extracellular space through exosomes is not yet determined. Therefore, here we aimed to investigate the effect of oxidative stress in bovine granulosa cells in vitro on the cellular and exosome mediated defense mechanisms...
2017: PloS One
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