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https://www.readbyqxmd.com/read/28436987/genomic-consequences-of-aberrant-dna-repair-mechanisms-stratify-ovarian-cancer-histotypes
#1
Yi Kan Wang, Ali Bashashati, Michael S Anglesio, Dawn R Cochrane, Diljot S Grewal, Gavin Ha, Andrew McPherson, Hugo M Horlings, Janine Senz, Leah M Prentice, Anthony N Karnezis, Daniel Lai, Mohamed R Aniba, Allen W Zhang, Karey Shumansky, Celia Siu, Adrian Wan, Melissa K McConechy, Hector Li-Chang, Alicia Tone, Diane Provencher, Manon de Ladurantaye, Hubert Fleury, Aikou Okamoto, Satoshi Yanagida, Nozomu Yanaihara, Misato Saito, Andrew J Mungall, Richard Moore, Marco A Marra, C Blake Gilks, Anne-Marie Mes-Masson, Jessica N McAlpine, Samuel Aparicio, David G Huntsman, Sohrab P Shah
We studied the whole-genome point mutation and structural variation patterns of 133 tumors (59 high-grade serous (HGSC), 35 clear cell (CCOC), 29 endometrioid (ENOC), and 10 adult granulosa cell (GCT)) as a substrate for class discovery in ovarian cancer. Ab initio clustering of integrated point mutation and structural variation signatures identified seven subgroups both between and within histotypes. Prevalence of foldback inversions identified a prognostically significant HGSC group associated with inferior survival...
April 24, 2017: Nature Genetics
https://www.readbyqxmd.com/read/28432272/establishment-of-an-in-vitro-culture-model-of-theca-cells-from-hierarchical-follicles-in-ducks
#2
Xiang Gan, da Chen, Yan Deng, Junsong Yuan, Bo Kang, Jiamin Qiu, Wenqiang Sun, Chunchun Han, Jiwei Hu, Liang Li, Jiwen Wang
Theca cells, including theca interna cells and theca externa cells, are vital components of ovarian follicles. The aim of this study is to identify a reliable method for the in vitro culture of theca cells from duck ovarian hierarchical (F4-F2) follicles. We improved the method for cell separation by using trypsin to further remove granular cells, and we increased the concentration of fetal bovine serum used in in vitro culture to improve cytoactivity. Cell antibody immunofluorescence showed that all inoculated cells could be stained by the CYP17A1/19A1 antibody but not by the FSHR antibody, which could stain granulosa cells...
April 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28420520/the-association-of-enchondromatosis-with-malignant-transformed-chondrosarcoma-and-ovarian-juvenile-granulosa-cell-tumor-ollier-disease
#3
Andrea Burgetova, Zdenek Matejovsky, Michal Zikan, Jiri Slama, Pavel Dundr, Petr Skapa, Kamila Benkova, David Cibula, Daniela Fischerova
OBJECTIVE: Ovarian juvenile granulosa cell tumor has an interesting association with multiple enchondromatosis (Ollier disease and Maffucci syndrome) and should be considered a leading diagnosis when an ovarian mass is found in young patients with these conditions. Besides the association with nonskeletal malignancies, there is a high risk of malignant transformation of enchondroma in chondrosarcoma as was also the case in this instance. CASE REPORT: The report uses multiple images to document the representative and characteristic markers of multiple enchondromas in a 22-year-old patient with Ollier disease complicated by malignant transformation of chondrosarcoma and in whom the disease is associated with ovarian juvenile granulosa cell tumor of the right ovary...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28420480/comparative-analysis-of-granulosa-cell-gene-expression-in-association-with-oocyte-competence-in-fsh-stimulated-holstein-cows
#4
David A Landry, Chloé Fortin, Anne-Marie Bellefleur, Rémi Labrecque, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Marc-André Sirard
Ovarian stimulation with exogenous FSH followed by FSH withdrawal or 'coasting' is an effective means of increasing the number of oocytes obtainable for the in vitro production of cattle embryos. However, the quality of the oocytes thus obtained varies considerably from one cow to the next. The aim of the present study was to gain a better understanding of the follicular conditions associated with low oocyte developmental competence. Granulosa cells from 94 Holstein cows in a commercial embryo production facility were collected following ovarian stimulation and coasting...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28420163/beneficial-effects-of-melatonin-on-the-in-vitro-maturation-of-sheep-oocytes-and-its-relation-to-melatonin-receptors
#5
Xiuzhi Tian, Feng Wang, Lu Zhang, Changjiu He, Pengyun Ji, Jing Wang, Zhenzhen Zhang, Dongying Lv, Wusiman Abulizi, Xuguang Wang, Zhengxing Lian, Guoshi Liu
(1) Background: The binding sites of melatonin, as a multifunctional molecule, have been identified in human, porcine, and bovine samples. However, the binding sites and mechanisms of melatonin have not been reported in sheep; (2) Methods: Cumulus-oocyte complexes (COCs) were cultured in TCM-199 supplemented with melatonin at concentrations of 0, 10(-3), 10(-5), 10(-7), 10(-9), and 10(-11) M. Melatonin receptors (MT1 and MT2) were evaluated via immunofluorescence and Western blot. The effects of melatonin on cumulus cell expansion, nuclear maturation, embryo development, and related gene (GDF9, DNMT1, PTX3, HAS2, and EGFR) expression were investigated...
April 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28412504/endotoxin-induced-tlr4-signaling-downregulates-cyp19a1-expression-through-cebpb-in-buffalo-granulosa-cells
#6
Vengala Rao Yenuganti, Ravinder, Dheer Singh
Estrogen is essential for growth and development of ovarian follicles. Infections associated with E. coli or Endotoxin (LPS) suppress estradiol production by the downregulation of CYP19A1 expression. However, the molecular mechanism of its down regulation is not yet known. To elucidate the molecular mechanisms of LPS-mediated downregulation of CYP19A1 gene expression, we studied the effect of LPS and TLR4 signaling pathway inhibitor (OxPAPC, OxPAPC-Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine) on CYP19A1 expression, and expression of CEBPB and CEBPB binding on CYP19A1 proximal promoter (CYP19A1 PII) in buffalo granulosa cells in vitro...
April 13, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28410456/use-of-bone-morphogenetic-protein-15-polymorphisms-to-predict-ovarian-stimulation-outcomes-in-infertile-brazilian-women
#7
Carla Peluso, Cecilia Goldman, Viviane Cavalcanti, Guilherme Gastaldo, Camila Martins Trevisan, Denise Maria Christofolini, Caio Parente Barbosa, Bianca Bianco
AIMS: Polymorphisms in the gene encoding bone morphogenetic protein 15 (BMP15) can result in inhibited secretion or lowered bioactivity of the BMP15 protein. BMP15 levels are associated with follicle-stimulating hormone receptor (FSHR) action on granulosa cells, wherein FSHR increases the sensitivity of ovarian follicles to follicle-stimulating hormone (FSH). In this study we evaluated the BMP15 polymorphisms A905 > G/rs3897937, C901 > T/rs17003221, and C-9 > G/rs3810682 in infertile Brazilian women in terms of anti-Mullerian hormone (AMH), FSH, and estradiol serum levels, as well as controlled ovarian hyperstimulation response and assisted reproduction outcomes...
April 14, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28410121/nitric-oxide-signaling-during-meiotic-cell-cycle-regulation-in-mammalian-oocytes
#8
Meenakshi Tiwari, Shilpa Prasad, Ashutosh N Pandey, Karuppanan V Premkumar, Anima Tripathi, Anumegha Gupta, Doddalingaiah R Chetan, Pramod K Yadav, Tulsidas G Shrivastav, Shail K Chaube
  Nitric oxide (NO) acts as a major signal molecules and modulate physiology of mammalian oocytes. Ovarian follicles generate large amount of NO through nitric oxide synthase (NOS) pathway to maintain diplotene arrest in preovulatory oocytes. Removal of oocytes from follicular microenvironment or follicular rupture during ovulation disrupt the flow of NO from granulosa cells to the oocyte that results a transient decrease of oocyte cytoplasmic NO level. Decreased NO level reduces cyclic nucleotides level by inactivating guanylyl cyclases directly or indirectly...
June 1, 2017: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28409689/effects-of-methoxychlor-on-igf-i-signaling-pathway-in-rat-ovary
#9
O Ozden-Akkaya, K Altunbas, A Yagcı
Follicular development and other ovarian functions are regulated by growth factors that can be affected by exogenous agents. Methoxychlor (MXC) is an organochloride pesticide that causes female infertility. We investigated how MXC affects the distribution of developing ovarian follicles in adult rats after treatment between embryonic day (E) 18 and postnatal day (PND) 7. We also measured insulin-like growth factor-I (IGF-I) and its receptor, IGF-IR, expressions in ovarian follicles and investigated whether MXC changed the levels of IGF-I and IGF-IR in the ovary...
2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28407862/lipid-profiles-of-follicular-fluid-from-cows-submitted-to-ovarian-superstimulation
#10
P H Santos, P K Fontes, F F Franchi, M F G Nogueira, K R A Belaz, A Tata, M N Eberlin, M J Sudano, C M Barros, A C S Castilho
Ovarian superstimulation with exogenous gonadotropins has been extensively used to produce in vivo-derived embryos for embryo transfer in cattle. This process modifies the antral follicle microenvironment and affects oocyte and embryo quality as well the differentiation of granulosa cells. Lipids play significant roles in the cell, such as energy storage, cell structure, and fine-tuning of the physical properties and functions of biological membranes. The phospholipid (PL) contents as well as the effects of superstimulatory treatments on the PL profile of follicular fluid from cows, however, remain unknown...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28407051/igf1r-expression-in-ovarian-granulosa-cells-is-essential-for-steroidogenesis-follicle-survival-and-fertility-in-female-mice
#11
Sarah C Baumgarten, Marah Armouti, CheMyong Ko, Carlos Stocco
Folliculogenesis is a lengthy process that requires the proliferation and differentiation of granulosa cells (GCs) for preovulatory follicle formation. The most crucial endocrine factor involved in this process is follicle-stimulating hormone (FSH). Interestingly, previous in vitro studies indicated that FSH fails to stimulate GC proliferation in the absence of the insulin-like growth factor 1 receptor (IGF1R). To determine the role of the IGF1R in vivo, female mice with a conditional knockdown of the IGF1R in the GCs were produced and had undetectable levels of IGF1R mRNA and protein in the GCs...
April 12, 2017: Endocrinology
https://www.readbyqxmd.com/read/28406912/the-elusive-maestro-gene-its-human-reproductive-tissue-specific-expression-pattern
#12
Shlomit Kenigsberg, Patricia D A Lima, Leila Maghen, Brandon A Wyse, Chantal Lackan, Annie N Y Cheung, Benjamin K Tsang, Clifford L Librach
The encoded transcript of the Maestro-Male-specific Transcription in the developing Reproductive Organs (MRO) gene exhibits sexual dimorphic expression during murine gonadal development. The gene has no homology to any known gene and its expression pattern, protein function or structure are still unknown. Previously, studying gene expression in human ovarian cumulus cells, we found increased expression of MRO in lean-type Polycystic Ovarian Syndrome (PCOS) subjects, as compared to controls. In this study, we examined the MRO splice variants and protein expression pattern in various human tissues and cells...
2017: PloS One
https://www.readbyqxmd.com/read/28403963/the-wnt-%C3%AE-catenin-signaling-pathway-may-be-involved-in-granulosa-cell-apoptosis-from-patients-with-pcos-in-north-china
#13
X-Q Wu, Y-Q Wang, S-M Xu, J-F Liu, X-Y Bi, Z-Q Wang, J-P Zhang
INTRODUCTION: Investigate the expressions of WNTs in granulosa cells of PCOS in North China, and explore the possible mechanism. MATERIAL AND METHODS: Patients with PCOS (n=40) and controls without PCOS (n=20) were enrolled into the study. The levels of serum sex hormone were detected by chemiluminescent enzyme immunoassay. RT-qPCR was used to measure mRNA levels of WNT family members (WNT1, WNT3, WNT4, WNT5, sFRP4 and sFRP5). The levels of Bax and Bcl-2 were measured by enzyme-linked immunosorbent assay...
January 2017: J Gynecol Obstet Hum Reprod
https://www.readbyqxmd.com/read/28403200/identification-of-potential-biomarkers-in-donor-cows-for-in-vitro-embryo-production-by-granulosa-cell-transcriptomics
#14
Gianluca Mazzoni, Suraya M Salleh, Kristine Freude, Hanne S Pedersen, Lotte Stroebech, Henrik Callesen, Poul Hyttel, Haja N Kadarmideen
The Ovum Pick Up-In vitro Production (OPU-IVP) of embryos is an advanced reproductive technology used in cattle production but the complex biological mechanisms behind IVP outcomes are not fully understood. In this study we sequenced RNA of granulosa cells collected from Holstein cows at oocyte aspiration prior to IVP, to identify candidate genes and biological mechanisms for favourable IVP-related traits in donor cows where IVP was performed separately for each animal. We identified 56 genes significantly associated with IVP scores (BL rate, kinetic and morphology)...
2017: PloS One
https://www.readbyqxmd.com/read/28402544/angiotensin-1-7-in-human-follicular-fluid-correlates-with-oocyte-maturation
#15
Ines K Cavallo, Cynthia Dela Cruz, Marilene L Oliveira, Helen L Del Puerto, Júlia A Dias, Veronica N Lobach, Maíra Casalechi, Maria G Camargos, Adelina M Reis, Robson A Santos, Fernando M Reis
STUDY QUESTION: Do angiotensin (Ang)-(1-7) levels in human ovarian follicular fluid (FF) correlate with the number and proportion of mature oocytes obtained for IVF? SUMMARY ANSWER: The present study shows for the first time that Ang-(1-7) levels in human FF correlate with the proportion of mature oocytes collected upon ovarian stimulation for IVF. WHAT IS KNOWN ALREADY: Ang-(1-7) is an active peptide of the renin-angiotensin system that stimulates oocyte maturation in isolated rabbit and rat ovaries...
April 10, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28402007/interactions-of-bovine-oocytes-with-follicular-elements-with-respect-to-lipid-metabolism
#16
Ewelina Warzych, Piotr Pawlak, Marcin Pszczola, Adam Cieslak, Zofia E Madeja, Dorota Lechniak
Among many factors, lipid metabolism within the follicular environment emerges as an important indicator of oocyte quality. In the literature a crucial significance is described concerning follicular fluid (FF) composition as well as messenger RNA (mRNA) expression in follicular cells. The aim of this study was to describe the relationship between oocyte, FF and follicular cells with regard to lipid metabolism. The set of data originating from individual follicles comprised: lipid droplets (LD) number in oocytes (BODIPY staining), mRNA expression of seven genes in cumulus and granulosa cells (SCD, FADS2, ELOVL2, ELOVL5, GLUT1, GLUT3, GLUT8; real time polymerase chain reaction) and fatty acid (FA) composition in FF (gas chromatography)...
April 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28400616/granulosa-cell-endothelin-2-expression-is-fundamental-for-ovulatory-follicle-rupture
#17
Joseph A Cacioppo, Po-Ching Patrick Lin, Patrick R Hannon, Daniel R McDougle, Arnon Gal, CheMyong Ko
Ovulation is dependent upon numerous factors mediating follicular growth, vascularization, and ultimately oocyte release via follicle rupture. Endothelin-2 (EDN2) is a potent vasoconstrictor that is transiently produced prior to follicle rupture by granulosa cells of periovulatory follicles and induces ovarian contraction. To determine the role of Edn2 expression, surgical transplant and novel conditional knockout mice were super-ovulated and analyzed. Conditional knockout mice utilized a new iCre driven by the Esr2 promoter to selectively remove Edn2...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28400072/notch-signaling-pathway-promotes-the-development-of-ovine-ovarian-follicular-granulosa-cells
#18
Jiongjie Jing, Xiaolong Jiang, Jianwei Chen, Xiaolei Yao, Miaomiao Zhao, Pengfei Li, Yangyang Pan, Youshe Ren, Wenzhong Liu, Lihua Lyu
The Notch signaling pathway regulates cell proliferation, differentiation and apoptosis involved in development of the organs and tissues such as nervous system, cartilage, lungs, kidneys and prostate as well as the ovarian follicles. This study aimed to investigate the mRNA expression and localization of NOTCH2, as the key factor in Notch signaling pathway. This was determined by PCR, real-time PCR and immunohistochemistry. Additionally, the effects of inhibiting Notch signaling pathway with different concentrations (5μM, 10μM and 20μM) of N-[N-(3, 5-Difuorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT), an inhibitor of Notch signaling pathway, on ovine granulosa cells was determined in vitro by detecting estradiol production using enzyme linked immunosorbent assay and expressions of the genes related to the cell cycle and apoptosis using real-time polymerase chain reaction (PCR)...
March 28, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28397811/a-novel-action-of-follicle-stimulating-hormone-in-the-ovary-promotes-estradiol-production-without-inducing-excessive-follicular-growth-before-puberty
#19
Charlotte M François, Florence Petit, Frank Giton, Alain Gougeon, Célia Ravel, Solange Magre, Joëlle Cohen-Tannoudji, Céline J Guigon
In cyclic females, FSH stimulates ovarian estradiol (E2) production and follicular growth up to the terminal stage. A transient elevation in circulating FSH and E2 levels occurs shortly after birth. But what could be the action of FSH on the ovary during this period, and in particular how it stimulates ovarian steroidogenesis without supporting terminal follicular maturation is intriguing. By experimentally manipulating FSH levels, we demonstrate in mice that the mid-infantile elevation in FSH is mandatory for E2 production by the immature ovary, but that it does not stimulate follicle growth...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28394173/sheep-isolated-secondary-follicles-are-able-to-produce-metaphase-ii-oocytes-after-vitrification-and-long-term-in-vitro-growth
#20
Franciele Osmarini Lunardi, Francisco Leo Nascimento de Aguiar, Livia Brunetti Apolloni, Ana Beatriz Graça Duarte, Naiza Arcângela Ribeiro de Sá, Érica Suzanne Soares Leal, Antonia Debora Sales, Carlos Henrique Lobo, Cláudio Cabral Campello, Johan Smitz, Gary Allen Apgar, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues
The vitrification of preantral follicles followed by in vitro growth (IVG) could be valuable to produce fertilizable oocytes. However, the meiotic resumption rates of oocytes cultured from vitrified secondary follicles (SF) have been reported as suboptimal. This study aimed to verify two base media (alpha modification of minimum essential medium, α-MEM, and tissue culture medium 199, TCM199) on vitrified SF regarding different requirements during IVG. Sheep ovarian fragments were divided in six groups: (1) Fresh groups (Control α-MEM and TCM199): SF without vitrification; (2) Follicle-Vitrified (Follicle-Vit α-MEM and TCM199): SF vitrified after isolation; and (3) Tissue-Vitrified (Tissue-Vit α-MEM and TCM199): SF vitrified enclosed in ovarian fragments and, subsequently, isolated...
April 10, 2017: Biopreservation and Biobanking
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