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Alice Luddi, Martina Gori, Camilla Marrocco, Angela Capaldo, Valentina Pavone, Laura Bianchi, Letizia Boschi, Giuseppe Morgante, Paola Piomboni, Vincenzo de Leo
OBJECTIVE: To study the molecular profile of metalloproteinases and their tissue inhibitors in granulosa and cumulus cells in a subset of fertile and infertile women. DESIGN: Molecular study with granulosa and cumulus cells. SETTING: University hospital. PATIENT(S): Forty-four women undergoing assisted reproductive techniques for female infertility factor, with partners having a normal spermiogram and 15 normally fertile women with male partner affected by severe oligoasthenoteratozoospermia or nonobstructive azoospermia...
May 2018: Fertility and Sterility
Ruoxi Jing, Lantao Gu, Jinqiu Li, Yanzhang Gong
Previous studies have shown that theca and granulosa cell layers in follicles do not play the same roles in mammals and birds, especially regarding the synthesis of estrogen. The functions of these two cell types have been well characterized in cattle, but they remain unclear in chickens. To clarify this issue, a comparison of small yellow follicles (SYFs) in chickens and cattle at different follicular development stages was done by weighted gene co-expression network analysis (WGCNA). The modules obtained from WGCNA were used for further identification of the key genes associated with CYP19A1 expression...
April 28, 2018: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
Emmi Rotgers, Anne Jørgensen, Humphrey Hung-Chang Yao
The reproductive endocrine systems are vastly different between male and female. This sexual dimorphism of endocrine milieu originates from sex-specific differentiation of the somatic cells in the gonads during fetal life. The majority of gonadal somatic cells arise from the adrenogonadal primordium. After separation of the adrenal and gonadal primordia, the gonadal somatic cells initiate sex-specific differentiation during gonadal sex determination with the specification of the supporting cell lineages: Sertoli cells in the testis vs...
May 15, 2018: Endocrine Reviews
C O Rosa, Lsr Marinho, Pra da Rosa, M P De Cesaro, P A Lunardelli, K C Silva-Santos, A C Basso, V Bordignon, M M Seneda
The aims of this study were to investigate whether the number of antral follicles (AF) in the ovaries of Nelore cows is influenced with the developmental competence of oocytes to reach the blastocyst stage and to quantify the mRNA abundance of genes associated with folliculogenesis and oogenesis in granulosa and cumulus cells. A total of 168 cows were distributed into two experimental groups according to the number of AF, low (≤31) and high AF (≥92), which were determined based on the mean number of AF (61...
May 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Li Meng, Sabrina Z Jan, Geert Hamer, Ans M van Pelt, Inge van der Stelt, Jaap Keijer, Katja J Teerds
There is general agreement that granulosa cell apoptosis is the cause of antral follicle attrition. Less clear is whether this pathway is also activated in case of preantral follicle degeneration, as several reports mention that the incidence of granulosa cell apoptosis in preantral follicles is negligible. Our objective is therefore to determine which cell-death pathways are involved in preantral and antral follicular degeneration.Atretic preantal and antral follicles were investigated using immunohistochemistry and laser-capture micro-dissection followed by qRT-PCR...
May 14, 2018: Biology of Reproduction
Ferit Kerim Kucukler, Umit Gorkem, Yasin Simsek, Ramazan Kocabas, Serdar Guler
Introduction: Thyroid dysfunction is among the most common autoimmune disorders in women of reproductive age. Previous studies have shown the association between autoimmune thyroid disease (AITD) and infertility. Anti-Müllerian hormone (AMH) is secreted by granulosa cells and is a useful marker for assessment of ovarian reserve. In the present study, we sought to evaluate the ovarian reserves of women with autoimmune thyroid disorder by measurement of AMH values. Material and methods: This prospective study included women with newly diagnosed AITD aged between 20 and 40 years...
April 2018: Archives of Medical Science: AMS
Guo-Liang Zhang, Jun-Lin Song, Yi Zhou, Rui-Qian Zhang, Shun-Feng Cheng, Xiao-Feng Sun, Guo-Qing Qin, Wei Shen, Lan Li
Zearalenone (ZEA), a natural contaminant found in feed, has been shown to have a negative impact on domestic animal reproduction, particularly in pigs. There are species-specific differences in the ZEA-induced toxicity pattern. Here, we investigated the different biological effects of ZEA exposure on porcine and mouse granulosa cells, using RNA-seq analysis. We treated murine and porcine granulosa cells with 10 μM and 30 μM ZEA during 72 h of culturing, in vitro. The results showed that 10 μM ZEA exposure significantly altered mitosis associated genes in porcine granulosa cells, while the same treatment significantly altered the steroidogenesis associated genes in mouse granulosa cells...
May 11, 2018: Toxicology and Applied Pharmacology
Sandro C Esteves, Gautam Khastgir, Jatin Shah, Kshitiz Murdia, Shweta Mittal Gupta, Durga G Rao, Soumyaroop Dash, Kundan Ingale, Milind Patil, Kunji Moideen, Priti Thakor, Pavitra Dewda
Progesterone elevation (PE) during the late follicular phase of controlled ovarian stimulation in fresh embryo transfer in vitro fertilization (IVF)/intracytoplasmic sperm injection cycles has been claimed to be associated with decreased pregnancy rates. However, the evidence is not unequivocal, and clinicians still have questions about the clinical validity of measuring P levels during the follicular phase of stimulated cycles. We reviewed the existing literature aimed at answering four relevant clinical questions, namely (i) Is gonadotropin type associated with PE during the follicular phase of stimulated cycles? (ii) Is PE on the day of human chorionic gonadotropin (hCG) associated with negative fresh embryo transfer IVF/intracytoplasmic sperm injection (ICSI) cycles outcomes in all patient subgroups? (iii) Which P thresholds are best to identify patients at risk of implantation failure due to PE in a fresh embryo transfer? and (iv) Should a freeze all policy be adopted in all the cycles with PE on the day of hCG? The existing evidence indicates that late follicular phase progesterone rise in gonadotropin releasing analog cycles is mainly caused by the supraphysiological stimulation of granulosa cells with exogenous follicle-stimulating hormone...
2018: Frontiers in Endocrinology
Xin-Yue Zhang, Hsun-Ming Chang, Elizabeth L Taylor, Peter C K Leung, Rui-Zhi Liu
Bone morphogenetic protein 6 (BMP6) is a critical regulator of follicular development that is expressed in mammalian oocytes and granulosa cells. Glial cell line-derived neurotrophic factor (GDNF) is an intraovarian neurotrophic factor that plays an essential role in regulating mammalian oocyte maturation. The aim of this study was to investigate the effect of BMP6 on the regulation of GDNF expression and the potential underlying mechanisms. We used an established immortalized human granulosa cell line (SVOG cells) and primary human granulosa-lutein cells as in vitro cell models...
May 9, 2018: Endocrinology
Saeid Nekoonam, Mohammad Naji, Keywan Mortezaee, Fardin Amidi
AR-mediated androgen signaling plays a key role in female reproductive system. Granulosa-lutein cells (GCs) are the main sites for expression of androgen receptor (AR). There is also a close relation between AKT signaling and AR. Here, we assayed the role for a synthetic AR ligand methyltrienolone (R1881) in expressions of AKTs and AR. Controlled ovarian hyperstimulation (COH) was performed in 20 normal women. Mural GCs were isolated by filtration method, cultured, and passaged. Then, the cells were starved for 48 h with 10% charcoal stripped FBS...
May 11, 2018: Journal of Cellular Biochemistry
Adelina M Reis, Kinulpe Honorato-Sampaio
The polycystic ovary (PCO) syndrome (PCOS) is the most common cause of anovulatory infertility in women and is associated with several clinical disorders. Despite the great amount of research in the area, mechanisms involved in the genesis of this syndrome remain poorly understood. In a recent issue of Clinical Science (vol. 132, issue 7, 759-776), Wang and colleagues, highlight the important role of overactivated C-type natriuretic peptide (CNP) and natriuretic peptide receptor 2 (CNP/NPR2) system in preventing oocyte maturation and ovulation in PCOS mice model induced by androgen...
May 16, 2018: Clinical Science (1979-)
Qin Zheng, Feifei Zhou, Xinyuan Cui, Mulin Liu, Yulin Li, Shuai Liu, Jichun Tan, Qiu Yan
BACKGROUND/AIMS: Polycystic ovary syndrome (PCOS), characterized by female infertility and metabolic abnormalities, is one of the most common endocrine disorders. The etiology of PCOS remains unknown. The comprehensive analysis of protein alterations in PCOS patients is meaningful for identifying diagnostic biomarkers of PCOS. Here, we explored the clinical value of serum proteins as novel biomarkers to detect PCOS with low progesterone level. METHODS: A total of 43 patients with PCOS and 30 healthy women were enrolled...
May 3, 2018: Cellular Physiology and Biochemistry
Xiaowei Nie, Wenjie Sheng, Daorong Hou, Qiang Liu, Ronggen Wang, Yong Tan
AIM OF THE STUDY: This study was designed to investigate the effect of different concentrations of Hyperin and Icariin (ICA)on proliferation and the secretion of estrogen (E2), and progesterone (P) in granulosa cells, and to explore the effect of Hyperin and Icariin on the expression of CYP17 and CYP19. MATERIALS AND METHODS: Rat ovary granulosa cells were cultured in vitro and treated with different concentrations of Hyperin and Icariin. The proliferation of ovarian granulosa cells was measured with the MTT assay...
May 2, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Ye Chen, Fenghua Liu, Yingqi Nong, Jianxing Ruan, Qingqing Guo, Ming Luo, Qianwen Huang
OBJECTIVES: To investigate the clinical efficacy of growth hormone (GH) in patients experiencing repeat implantation failure (RIF) and explore the possible mechanism. METHODS: Forty-two RIF patients undergoing in vitro fertilization-embryo transfer (IVF-ET) were enrolled in the present trial: 22 patients who received GH (treatment group) and 20 who did not receive GH (controls). RESULTS: The clinical pregnancy and live birth rates in the treatment group were significantly higher than those in the control group (both P<0...
May 4, 2018: Canadian Journal of Physiology and Pharmacology
Qiqi Li, Xing Du, Zengxiang Pan, Lifan Zhang, Qifa Li
The cytochrome P450 family 19 subfamily A member 1 (CYP19A1) gene, encodes aromatase, a key enzyme in estradiol (E2) synthesis, and is down-regulated during porcine follicular atresia. However, its role in and the mechanism of transcriptional repression in follicular atresia is largely unknown. In the present study, we show that the CYP19A1 gene stimulates E2 release and inhibits cell apoptosis in porcine granulosa cells (GCs). SMAD4, an anti-apoptotic moderator, was identified as a transcription factor of the porcine CYP19A1 gene and enhanced the expression and function of CYP19A1 in porcine GCs through direct binding to a SMAD4-binding element (SBE) within the promoter region of CYP19A1 gene...
April 30, 2018: Molecular and Cellular Endocrinology
Peng Han, Hilda Guerrero-Netro, Anthony Estienne, Christopher A Price
The theca cell layer of the ovarian follicle secretes growth factors that impact the function of granulosa cells. One such factor is fibroblast growth factor 18 (FGF18) that causes apoptosis of granulosa cells, however it is not known if FGF18 induces apoptosis also in theca cells. Addition of recombinant FGF18 to bovine theca cells in vitro inhibited steroidogenesis but, in contrast to previous data in granulosa cells, decreased the incidence of apoptosis. FGF18 activated typical FGF signaling pathways in theca cells, which was not previously observed in granulosa cells...
May 1, 2018: Molecular and Cellular Endocrinology
Lei Du, Tiantian Gu, Yang Zhang, Zhengyang Huang, Wenming Zhao, Guobin Chang, Qi Xu, Guohong Chen, Ningzhao Wu
1. The low reproductive performance of geese has seriously hampered the development of the industry. Reproductive performance, particularly the egg laying rate mainly depends on the development of the follicle. Previous studies have shown that follicle-stimulating hormone (FSH) plays an important role in the process of follicular development, but the exact underlying mechanism remains unclear. 2. This study showed that FSH-stimulated granulosa cell proliferation in a dose-dependent manner. The effect of FSH treatment on granulosa cell proliferation was greatest at a dose of 100 mIU/ml FSH for 24 h...
May 3, 2018: British Poultry Science
Hari Om Pande, Dawit Tesfaye, Michael Hoelker, Samuel Gebremedhn, Eva Held, Christiane Neuhoff, Ernst Tholen, Karl Schellander, Dessie Salilew Wondim
BACKGROUND: The granulosa cells are indispensable for follicular development and its function is orchestrated by several genes, which in turn posttranscriptionally regulated by microRNAs (miRNA). In our previous study, the miRRNA-424/503 cluster was found to be highly abundant in bovine granulosa cells (bGCs) of preovulatory dominant follicle compared to subordinate counterpart at day 19 of the bovine estrous cycle. Other study also indicated the involvement of miR-424/503 cluster in tumour cell resistance to apoptosis suggesting this miRNA cluster may involve in cell survival...
May 1, 2018: Journal of Ovarian Research
Jennifer N Stall, Robert H Young
Sixteen adult granulosa cell tumors which had conspicuous zones of cells with pale cytoplasm imparting a resemblance to thecoma are reported. The neoplasms occurred in patients from 38 to 86 yr of age, the majority being over 55 yr of age. Ten tumors were incidental findings, the remainder being associated with symptoms or signs related to an adnexal mass. All the tumors were unilateral, typically small, usually under 5 cm, with only 3 being larger. With 1 exception they were uniformly solid and were typically entirely or focally yellow on sectioning...
April 27, 2018: International Journal of Gynecological Pathology
Wenju Liu, Shujuan Wang, Jinxing Zhou, Xunsheng Pang, Like Wang
Melatonin is well known as a powerful free radical scavenger and exhibits the ability to prevent cell apoptosis. In the present study, we investigated the role of melatonin and its receptor MTNR1B in regulating the function of bovine granulosa cells (GCs) and hypothesized the involvement of MTNR1B in mediating the effect of melatonin on GCs. Our results showed that MTNR1B knockdown significantly promoted GCs apoptosis but did not affect the cell cycle. These results were further verified by increasing the expression of pro-apoptosis genes ( BAX and CASP3 ), decreasing expression of the anti-apoptosis genes ( BCL2 and BCL-XL ) and anti-oxidant genes ( SOD1 and GPX4 ) without affecting cell cycle factors ( CCND1 , CCNE1 and CDKN1A ) and TP53 ...
2018: PeerJ
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